Life Sciences Commons^™

Influence Of The Ccr2-V64i Polymorphism On Human Immunodeficiency Virus Type 1 Coreceptor Activity And On Chemokine Receptor Function Of Ccr2b, Ccr3, Ccr5, And Cxcr4, Benhur Lee, Benjamin J. Doranz, Shalini Rana, Yanji Yi, Mario Mellado, Jose M. R. Frade, Carlos Martinez-A., Stephen J. O'Brien, Michael Dean, Ronald G. Collman, Robert W. Doms

Biology Faculty Articles

The chemokine receptors CCR5 and CXCR4 are used by human immunodeficiency virus type 1 (HIV-1) in conjunction with CD4 to infect cells. In addition, some virus strains can use alternative chemokine receptors, including CCR2b and CCR3, for infection. A polymorphism in CCR2 (CCR2-V64I) is associated with a 2- to 4-year delay in the progression to AIDS. To investigate the mechanism of this protective effect, we studied the expression of CCR2b and CCR2b-V64I, their chemokine and HIV-1 coreceptor activities, and their effects on the expression and receptor activities of the major HIV-1 coreceptors. CCR2b and CCR2b-V64I were expressed at …

The Chickpea Book : A Technical Guide To Chickpea Production, Stephen Loss, Neil Brandon, K H M. Siddique

Bulletins 4000 -

The area of chickpea production in Australia has expanded rapidly in recent years especially in south-western Australia. This has been partly brought about by the keen interest of farmers and a concerted research effort and industry development by Agriculture Western Australia, The Centre for Legumes in Mediterranean Agriculture (CLIMA) and other institutions, in partnership with the Grains Research and Development Corporation and other industry funding bodies. Private consultants, grain traders and other industry groups have also contributed to the expansion of the industry.

Much of the local knowledge generated by these research and development projects has been published in various …

Escherichia Coli O157:H7 Requires Intimin For Enteropathogenicity In Calves, Evelyn A. Dean-Nystrom, Brad T. Bosworth, Harley W. Moon, Alison D. O’Brien

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Enterohemorrhagic Escherichia coli (EHEC) strains require intimin to induce attaching and effacing (A/E) lesions in newborn piglets. Infection of newborn calves with intimin-positive or intimin-negative EHEC O157: H7 demonstrated that intimin is needed for colonization, A/E lesions, and disease in cattle. These results suggest that experiments to determine if intimin-based vaccines reduce O157:H7 levels in cattle are warranted.

Is Soil Temperature Better Than Air Temperature For Predicting Winter Wheat Phenology?, Gregory S. Mcmaster, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In predicting wheat (Triticum aestivum L.) phenology, logic suggests that basing thermal unit accumulation on near-surface soil temperature should give a better representation of shoot apex thermal unit accumulation than air temperature until internode elongation raises the apex above the soil surface. A study was undertaken to determine if predictions of winter wheat phenology are improved when based on measured near-surface soil temperature rather than air temperature. Air temperature 1.5 m above the soil surface and soil temperature at crown depth (the position of the shoot apex before stem elongation) were collected for 23 site-years across the U.S. Central …

Biodegradation Of Atrazine By Agrobacterium Radiobacter J14a And Use Of This Strain In Bioremediation Of Contaminated Soil, J. K. Struthers, K. Jayachandran, T. B. Moorman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 μg of [¹⁴C-U-ring]atrazine ml^-1 in 72 h with a concurrent increase in the population size from 7.9 x 10⁵ to 5.0 x 10⁷ cells ml^-1. Under these conditions cells mineralized the [ethyl-¹⁴C]atrazine …

Residual Effects Of Crop Residues On Grain Production And Selected Soil Properties, James F. Power, Paul Koerner, John W. Doran, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Returning crop residue improves water conservation and storage, nutrient availability, and crop yields. We have little knowledge, however, of the residual impacts of crop residues on soil and crop production. We hypothesized that residual impacts of crop residues vary with the amount of residues used. A 10-yr study near Lincoln, NE, evaluated the residual effects of an earlier 8-yr study of various crop residue amounts on crop growth and selected soil properties. From 1978 through 1985, crop residues were returned at 0,50, 100, and 150% of the quantity produced by the previous crop (averaging 0 to =6 Mg ha^{-1 …}

Localization Of Sodium, Potassium-Adenosine Triphosphatase In The Cerebral Cortex Of Developing Rats, Maki Ujiie

Loma Linda University Electronic Theses, Dissertations & Projects

Active transport via Na⁺, K⁺-ATPase plays an important role in establishing and maintaining proper ionic gradient across all mammalian cell membranes. A strict regulation of Na⁺, and K⁺ ion levels is especially important in excitable cells such as neurons and myocytes. Inhibition of the enzyme is associated with several pathological conditions and has serious detrimental effects on cerebral cortical cells. Yet the enzyme appears to be limited in amount and distribution in newborn rats. In this study, Na_⁺, K⁺- ATPase expression and distribution in early postnatal rats were studied …

Mutational Analysis Of Area, A Transcriptional Activator Mediating Nitrogen Metabolite Repression In Aspergillus Nidulans And A Member Of The “Streetwise” Gata Family Of Transcription Factors, Richard A. Wilson, Herbert N. Arst Jr.

Fungal Molecular Plant-Microbe Interactions

Details a mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the “streetwise” GATA family of transcription factors.

Some Corrections To "The Beetles Of Northeastern North America", Ross H. Arnett Jr.

Insecta Mundi

The following are some of the corrections that have been submitted, as noted in the text, for "Beetles of Northeastern North America," by Norville M. Downie and Ross H. Arnett, Jr. 1996, Gainesville: American Insect Projects, 1721 pp. Most of the following was sent by R. Lundgren (in 1996) of Archer, Florida.

A New Tribe, Genus, And Species Of Limnichid Beetle, Woolridgeus Perforatus, From Malaysia (Coleoptera: Limnichidae: Wooldridgeini), Paul J. Spangler

Insecta Mundi

A new tribe is established in the Limnichidae, Limnichinae, for a new genus and species, Wooldridgeus perforatus, from Sabah, Malaysia. Two autapomorphic characters distinguish Wooldridgeini from the two other tribes of the subfamily Limnichinae; 1) the mesotarsus and metatarsus join their respective tibia ventrally (medially) and preapically instead of the usual apical only connection and 2) a large pore, presumed to be an opening of a prothoracic gland, is present on each hypomeron. Distinguishing characters are illustrated by scanning electron micrographs and line drawings. A key to the three tribes included in the Limnichinae is provided.

Taxonomy Of The Simulium Perflavum Species-Group (Diptera: Simuliidae), With Description Of A New Species From Brazil, Neusa Hamada, Peter H. Adler

Insecta Mundi

The larva, pupa, male, and female of Simulium trombetense n. sp. are described and illustrated. This species was collected in the Brazilian Amazon region in the states of Amapa, Amazonas, Para, and Roraima near the edges of the crystalline basement-rock formation of the Pre-Cambrian Guiana Shield. Keys for larvae, pupae, males, and females of species in the Simulium perflavum Species-Group are presented, as well as new observations on adult, pupal, and larval characters. Evidence is given to support the species status of S. maroniense Floch and Abonnenc, previously considered synonymous with S. rorotaense Floch and Abonnenc.

A Review Of Goerita (Trichoptera: Goeridae), With Description Of A New Species, Charles R. Parker

Insecta Mundi

Goerita Ross is restricted to the Appalachian Mountains and Allegheny Plateau of eastern North America. A new species is described, bringing the total in the genus to three. Keys to larvae and adults are presented, and the distributions of the species are recorded.

A Review Of Nearctic And Some Related Anthribidae (Coleoptera), Barry D. Valentine

Insecta Mundi

Taxonomy, synonymy, distribution, and biologies of Nearctic (and a few Neotropical and Palearctic) Anthribidae are reviewed, new keys are provided, and four new genera and eleven new species are described. Allandrus Leconte, 1876 (=Tropiderinus Reitter, 1916). Anthribus Geoffrey, 1762 (=Pseudobrachytarsus Pierce, 1930). Araecerus Schoenherr, 1823 (=Araeocorynus Jekel, 1855); Araecerus coffeae Fabricius, 1801 (=Tropideres (Rhaphitropis) mateui Cobos, 1954). Brachycorynus n. gen., type species Tropideres rectus Leconte, 1876; congeneric: Homocloeus distentus Frieser, 1983 from Cuba and Florida, and B. hirsutus n. sp. from Texas. Choragus major n. sp., Ohio, etc., striolatus n. sp., Ohio, and exophthalmus n. sp., …

Nebraska Bird Review (September 1998) 66(3), Whole Issue

Nebraska Bird Review

A Half Century of Winter Bird Surveys in Lincoln and Scottsbluff ... 74

Summer Field Report, June to July, 1998, Introduction ... 84

Species Accounts ... 86

Book Review: Baby Bird Portraits by George Miksch Sutton ... 100

1998 Midwinter Eagle Survey and Ten-Year Summary Tables ... 101

Central Nebraska Public Power Facilities Eagle Count ... 108

Nebraska Bird Count for 1997 Addendum ... 110

"A Half-Century Of Winter Bird Surveys At Lincoln And Scottsbluff, Nebraska", Paul A. Johnsgard

Nebraska Bird Review

Since 1900, the National Audubon Society has sponsored annual "Christmas bird counts" during the two-week period encompassing Christmas; and as a result, long-term data on winter bird populations have accumulated, especially for some locations. The first two such counts in Nebraska were made in 1909 and in 1912 in Lincoln. While similar counts were made in Omaha from 1909 to 1911, no further counts were conducted elsewhere in the state until the 1940's. Beginning in 1947 and continuing to the present, an unbroken series of counts were made in Lincoln, usually by members of the University Place Bird Club, the …

Cloning And Expression Of The Listeria Monocytogenes Scott A Ptsh And Ptsi Genes, Coding For Hpr And Enzyme I, Respectively, Of The Phosphotransferase System, Douglas P. Christensen, Andrew K. Benson, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) utilizes high-energy phosphate present in PEP to drive the uptake of several different carbohydrates in bacteria. In order to examine the role of the PTS in the physiology of Listeria monocytogenes, we identified the ptsH and ptsI genes encoding the HPr and enzyme I proteins, respectively, of the PTS. Nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to HPr and enzyme I proteins from other organisms. Purified L. monocytogenes HPr overexpressed in Escherichia coli was also capable of complementing an HPr defect in heterologous extracts of Staphylococcus aureus ptsH …

Identification Of The Gene Encoding The Alternative Sigma Factor ΣB From Listeria Monocytogenes And Its Role In Osmotolerance, Lynne A. Becker, Mehmet Sevket Cetin, Robert W. Hutkins, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Listeria monocytogenes is well known for its robust physiology, which permits growth at low temperatures under conditions of high osmolarity and low pH. Although studies have provided insight into the mechanisms used by L. monocytogenes to allay the physiological consequences of these adverse environments, little is known about how these responses are coordinated. In the studies presented here, we have cloned the sigB gene and several rsb genes from L. monocytogenes, encoding homologs of the alternative sigma factor σ^B and the RsbUVWX proteins, which govern transcription of a general stress regulon in the related bacterium Bacillus subtilis. …

Feeding Preferences Of A Generalist Salt-Marsh Crab: Relative Importance Of Multiple Plant Traits, Steven C. Pennings, Thomas H. Carefoot, Erin L. Siska, Margo E. Chase, Teresa A. Page

Ocean Engineering and Marine Sciences Faculty Publications

Few studies have evaluated the relative importance of multiple plant traits to herbivore diet choice, especially with an experimental approach. Moreover, although circumstantial evidence points to plant toughness and silica content as important deter- minents of diet choice, few studies have experimentally demonstrated that these factors actually deter feeding by herbivores. We examined feeding preferences of a generalist salt- marsh crab, Armases cinereum, for all the common angiosperms in its habitat. We took an experimental approach to evaluating the importance of toughness, secondary chemistry, silica, salt, and protein in determining feeding preferences. Consumption of plants by Armases in two experiments …

Viola Pratincola Greene, Annette Kessler

Specimens by Name

No abstract provided.

Hexapod Herald - Vol. 10, No. 7, August 31, 1998

Hexapod Herald and Other Entomology Department Newsletters

Faculty news

Staff news

Graduate student news

Publications

From Cheri's desk

Classes offered this semester

Calendar of events

State of Nebraska travel reimbursement requirements

Bats Of The Antillean Island Of Grenada: A New Zoogeographic Perspective, Hugh H. Genoways, Carleton J. Phillips, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

The island of Grenada is the southernmost of the Lesser Antilles, lying 130 km north of Trinidad and 135 km north of the Venezuelan mainland. It measures 34 km north to south and 19 km east to west and has an area of 312 square km. Grenada and the Grenadines northward to Bequia stand on the large submarine Grenada Bank. At 183 m depth, the bank is 179 km long. The Grenadines cover the bank to its northern end, but the bank extends for 39 km south of Grenada with no islands. During the last Ice Age, Grenada and the …

Broussonetia Papyrifera Vent., John E. Ebinger

Specimens by Name

No abstract provided.

Cropwatch No. 98-21, Aug. 28,1998, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field reports...........184

Planting wheat too early...........
Disease potential...........185
Hessian fly threat...........185
Fly-safe planting dates...........185

Anhydrous safety...........186

Soybean SCN aid...........186

Weeds in alfalfa...........187

Prairie seminars...........187

Corn tour set...........188

Developmental Regulation Of A Cyclin-Dependent Kinase Inhibitor Controls Postembryonic Cell Cycle Progression In Caenorhabditis Elegans, Yang Hong, Richard Roy, Victor Ambros

Dartmouth Scholarship

C. elegans cki-1 encodes a member of the CIP/KIP family of cyclin-dependent kinase inhibitors, and functions to link postembryonic developmental programs to cell cycle progression. The expression pattern of cki-1::GFP suggests that cki-1 is developmentally regulated in blast cells coincident with G1, and in differentiating cells. Ectopic expression of CKI-1 can prematurely arrest cells in G1, while reducing cki-1 activity by RNA-mediated interference (RNAi) causes extra larval cell divisions, suggesting a role for cki-1 in the developmental control of

INTRODUCTION

The proper development of a multicellular organism requires the precise orchestration of cell proliferation and differentiation. Despite considerable progress toward …

The A326s Mutant Of G(Iα1) As An Approximation Of The Receptor-Bound State, Bruce A. Posner, Mark B. Mixon, Mark A. Wall, Stephen R. Sprang, Alfred G. Gilman

Biological Sciences Faculty Publications

Agonist-bound heptahelical receptors activate heterotrimeric G proteins by catalyzing exchange of GDP for GTP on their α subunits. In search of an approximation of the receptor-α subunit complex, we have considered the properties of A326S G(iα1), a mutation discovered originally in G(sα) (Iiri, T., Herzmark, P., Nakamoto, J. M., Van Dop, C., and Bourne, H. R. (1994) Nature 371, 164-168) that mimics the effect of receptor on nucleotide exchange. The mutation accelerates dissociation of GDP from the α(i1)β₁γ₂ heterotrimer by 250-fold. Nevertheless, affinity of mutant G(iα1) for GTPγS is high in the presence of Mg²⁺, …

Strategies For Sustainable Economic Development, Duane Olsen

Cornhusker Economics

During a conference highlighting "sustainable Communities," Margaret Thomas presented a paper based upon a 1994 research project designed to explore economic development strategies that would increase local prosperity and also protect the environment and protect natural resources. She described the economy and ecology as two sides of the same coin and proposed reconsidering human systems and industrial systems in the light of these two natural laws: 1) resources are to be used no faster than they are replenished, and 2) matter is ultimately recycled for reuse in biological or physical systems.

Meeting Minutes, August 18th, 1998, National Smokejumper Association Executive Committee

National Smokejumper Association Meeting Minutes

Agenda: Minutes: copies of 14 Jul 98 executive committee meeting.; Treasurers Report: Video fund in depth.; Measures taken to balance the record of money taken in with deposited to the Video Fund account.; Membership: large increase over last month's figure due to addition of 1998 rookies and over 100 of the Triple Nickel jumpers from 1945.; Old Business: proposals on membership.; Collecting money through affiliate memberships.; Delay in discussion of the minutes of the Annual Meeting.; NSA returned to the original provider.; Video Committee update.; Use of some government equipment without authorization.; New Business: expanding merchandise sales.; Discussion on obtaining …

Corn And Soybean Basis Patterns For Selected Locations In South Dakota: 1998, Bashir Qasmi, Lisa A. Johnson

Economics Research Reports (1969 - 2017)

For successful marketing and merchandising in commodity markets, it is important to understand the relationship between cash and futures contract prices. The principal measure for relating cash and futures price is local cash basis or "Basis''.

El Nino Southern Oscillation-Related Ocean-Atmosphere Coupling In The Western Equatorial Pacific, D. A. Mayer, Robert H. Weisberg

Marine Science Faculty Publications

Using 43 years of Comprehensive Ocean-Atmosphere Data: Set and related data for the period 1950-1992, an examination is made into the regional dependence of ocean-atmosphere coupling in relation to the El Nino-Southern Oscillation (ENSO). The cross correlation between sea surface temperature (SST) and sea level pressure (SLP) anomalies over the global tropics shows two patterns of significant negative correlation consistent with a local hydrostatic response of SLP to SST: (1) the eastern Pacific, where the correlation is symmetric about and largest on the equator, and (2) the western Pacific, where symmetric regions of-negative correlation are found off the equator, separated …

Cropwatch No. 98-20, Aug. 14, 1998, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Wheat videoconference........176

Predicting last irrigation........177

Map weed problems........180

Dry Bean Field Day........181

Herbicide Use Guide........181

Field updates........181

Planting alfalfa........182

GDD........182

Crop water use........182