Life Sciences Commons^™

Corals Of The South-West Indian Ocean Iv. The Hard Coral Family Faviidae Gregory, 1900 (Scleractinia: Faviina), Bernhard Riegl

Marine & Environmental Sciences Faculty Reports

This annotated check-list deals with the scleractinian coral family Faviidae in subtropical south-east Africa (the African mainland coast south of the Tropic of Capricorn). Material from the atoll Bassas da India in the Mozambique channel was included. Most species are of wide Indo-Pacific distribution. The faviid fauna of Mozambique was richer (28 species) than the South African fauna (21 species). The fauna of Bassas da India was similar to that on the African mainland coast with four additional species. South-east Africa has a moderately rich faviid fauna compared with other areas in the Indo-Pacific region. Field and laboratory characteristics are …

Bacillus Thuringiensis Hd-73 Spores Have Surface-Localized Cry1ac Toxin: Physiological And Pathogenic Consequences, Cheng Du, Kenneth Nickerson

Papers in Microbiology

Spores from Cry⁺ strains of Bacillus thuringiensis bound fluorescein isothiocyanate-labeled antibodies specific for the 65-kDa activated Cry 1Ac toxin, whereas spores from Bacillus cereus and Cry^- strains of B. thuringiensis did not. The Cry⁺ spores could be activated for germination by alkaline conditions (pH 10.3), whereas Cry^- spores could not. Once the surrounding exosporia had been removed or permeabilized, Cry⁺ spores were able to bind the toxin receptor(s) from insect gut brush border membrane vesicle preparations, and their germination rates were increased ca. threefold in the presence of brush border membrane vesicles. A model is …

Review Of Planting The Future: Developing An Agriculture That Sustains Land And Community Edited By Elizabeth A. R. Bird, Gordon L. Bultena, And John C. Gardner, Charles A. Francis

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Why should you care about agriculture? Planting the Future provides an eloquent description of the current state of this' most basic human endeavor so critical to survival. Based on a series of surveys and on-farm studies in the North Central and Western states, the book recognizes the bounty of our conventional agricultural industry. More importantly for the future, it details a series of critical problems in the environment, the distribution of economic benefits, and the social dislocation resulting from the consolidation of lands and heavy reliance on fossil fuels. Today's productivity and cheap food in the market have hidden expenses …

Evaluation Of Sampling Resolution In Coral Stable Isotope Records: A Case Study Using Records From New Caledonia And Tarawa, Terrence M. Quinn, Frederick W. Taylor, Thomas J. Crowley, Suzanne M. Link

Marine Science Faculty Publications

We have generated a 40‐year‐long, monthly stable isotope record from a Porites lutea coral collected offshore of Amedee, New Caledonia (22°S, 167°E) to investigate the relation between sampling resolution in coral isotope studies and retrieval of sea‐surface environmental information. We interpret the high correlation between our oxygen isotope record and a twenty‐year long sea‐surface temperature record at the monthly timescale (r=0.88) to indicate that our coral isotope record is an accurate monitor of environmental conditions offshore of Amedee. The character of the signal and the percent variance explained in the record at the annual band, at the quasi‐biennial oscillation band …

Fish & Wildlife News: October 1996

Fish and Wildlife News

Contents:
Putting Refuges on the Map: Service Celebrates National Wildlife Refuge Week
Statement by President Clinton on Signing the “Mollie Beattie Wilderness Area Act”
Service Mussels Forward with Ecosystem Approach to Fish and Wildlife Conservation
Service Helps Dedicate Illinois Wetland as 15th Wetland of International Importance
Service Proposes Youth Waterfowling Day; Approval of Bismuth-Tin Sho
Seven Departments Agree to Bolster Recreational Fisheries
Talented Toddler Takes Trout at Alaska “Kids Fishing Day”
Service Urges Boaters to “Pump and Dump”
Lions and Tigers and . . . Snakes–– O’Hare Exhibit Educates Travelers About Illegal Wildlife
Wolves Thriving in Yellowstone, No New Introductions …

Cloning Of Human Acetyl-Coa Carboxylase-Beta And Its Unique Features., Joohun Ha, Jung-Kee Lee, Kyung-Sup Kim, Lee A. Witters, Ki-Han Kim

Dartmouth Scholarship

Acetyl-CoA carboxylase, which has a molecular mass of 265 kDa (ACC-alpha), catalyzes the rate-limiting step in the biosynthesis of long-chain fatty acids. In this study we report the complete amino acid sequence and unique features of an isoform of ACC with a molecular mass of 275 kDa (ACC-beta), which is primarily expressed in heart and skeletal muscles. In these tissues, ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. ACC-beta contains an amino acid sequence at the N terminus which is about 200 amino acids long and may be uniquely related to the …

Louisiana Agriculture Magazine, Fall 1996, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

A Cytoplasmic Male Sterility-Associated Mitochondrial Protein Causes Pollen Disruption In Transgenic Tobacco, Shichuan He, Andre R. Abad, Stanton B. Gelvin, Sally Ann Mackenzie

Center for Plant Science Innovation: Faculty Publications

In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen development is a consequence of mitochondrial dysfunction. To provide definitive evidence that expression of an abnormal mitochondrial gene can interrupt pollen development, a CMS-associated mitochondrial DNA sequence from common bean, orf239, was introduced into the tobacco nuclear genome. Several transformants containing the orf239M gene constructs, with or without a mitochondrial targeting sequence, exhibited a semisterile or male-sterile phenotype. Expression of the gene fusions in transformed anthers was confirmed using RNA gel …

Expression And Characterization Of An Rna Capping Enzyme Encoded By Chlorella Virus Pbcv-1, C. Kiong Ho, James L. Van Etten, Stewart Shuman

Department of Plant Pathology: Faculty Publications

We report that the A103R protein of Chlorella virus PBCV-1 is an mRNA capping enzyme that catalyzes the transfer of GMP from GTP to the 5’ diphosphate end of RNA. This is a two-step reaction in which the enzyme first condenses with GTP to form a covalent enzyme-GMP intermediate and then transfers the GMP to an RNA acceptor to form a GpppN cap. Purified recombinant A103R is a 38-kDa monomer that lacks RNA (guanine-7-) methyltransferase activity. With respect to its size, amino acid sequence, and biochemical properties, A103R is more closely related to the yeast RNA guanylyltransferases than it is …

On The Portability Of Behavioral Vhdl-93, Krishnaprasad Thirunarayan, Robert Ewing

Kno.e.sis Publications

Goossens defined a structural operational semantics for a subset of VHDL-87 and proved that the parallelism present in VHDL is benign. We extend this work to include shared variables in VHDL-93 that changes the underlying semantic model. In the presence of shared variables, non-deterministic execution of VHDL-93 processes destroys the unique meaning property. We identify and characterize a class of portable VHDL-93 descriptions for which unique meaning property can be salvaged.

Competition And Facilitation: Contrasting Effects Of Artemisia Tridentata On Desert Vs Montane Pines, Ragan M. Callaway, Eh Delucia, Darrin Moore, R Nowak, W. H. Schlesinger, Brenda J. Moor

Biological Sciences Faculty Publications

Circumstantial evidence suggests that Artemisia tridentata may out-compete Pinus ponderosa and P. jefferyi for water at ecotones between shrub steppe and montane forest vegetation in the Great Basin. Other studies indicate that within the shrub steppe Artemisia may act as a nurse plant for a third species of pine, P. monophylla. We used field experiments to study these contrasting effects of Artemisia on P, ponderosa and P. monophylla within the contest of the distributional patterns in western Nevada of all three species on andesite, and on sites where hydrothermal activity has altered the andesite. At intermediate elevations in the Great …

Genetic And Phylogenetic Divergence Of Feline Immunodeficiency Virus In The Puma (Puma Concolor), Margaret A. Carpenter, Eric W. Brown, Melanie Culver, Warren E. Johnson, Jill Pecon-Slattery, Dulce Brousset, Stephen J. O'Brien

Biology Faculty Articles

Feline immunodeficiency virus (FIV) is a lentivirus which causes an AIDS-like disease in domestic cats (Felis catus). A number of other felid species, including the puma (Puma concolor), carry a virus closely related to domestic cat FIV. Serological testing revealed the presence of antibodies to FIV in 22% of 434 samples from throughout the geographic range of the puma. FIV-Pco pol gene sequences isolated from pumas revealed extensive sequence diversity, greater than has been documented in the domestic cat. The puma sequences formed two highly divergent groups, analogous to the clades which have been defined for …

Images 2.1 : An Integrated Model Of An Arid Grazing System, Z G. Yan, K M. Wang

Resource management technical reports

The name IMAGES stands for An Integrated Model of an Arid Grazing Ecological System. The model was initiated by Dr R. Hacker in 1987-8 as an activity to (1) evaluate alternative management strategies and (2) identify key ecological processes and research priorities in shrub rangelands of Western Australia. Version 1 of the model was published in Agriculture Systems in 1991 (Hacker et al. 1991) and here after will be referred to as IMAGES 1. IMAGES 1 is a vegetation model, capable of predicting the prob ility of recruitment and mortality of the desirable species in a given vegetation type under …

Modeling Interactions Of Browsing Predation, Infaunal Activity And Recruitment In Marine Soft-Sediment Habitats, Sara M. Lindsay, David S. Wethey, Sarah A. Woodin

Faculty Publications

In marine soft-sediment habitats, the sediment surface is altered by activities of sediment dwellers (infauna). Such biogenic disturbance can influence recruitment success if settling larvae and juveniles avoid disturbed sites or if juveniles die as a result of disturbance after settling. Because infauna commonly lose exposed body parts to browsing predators and disturb less sediment as a result, we developed a simulation model to examine the interactions between browsing predation, infaunal adult activity, and recruitment. Sediment disturbance in the model was based on data for the polychaete Abarenicola pacifica. We simulated the activity of two general types of predators: prey …

Fire Management Of Spinifex Pastures In The Coastal And West Pilbara, John Stretch

Agriculture reports

Spinifex dominated plant communities are a major natural pasture resource in the Pilbara. Spinifex is noted for its ridged, hard and generally sharp pointed leaf blades, although individual species vary in this respect. Triodia pungens and T. schinzii are often referred to as soft spinifex. Soft spinifex is useful as stock feed in its own right, whereas 'hard' spinifex species are rarely grazed by stock. Soft spinifex dominated pastures are relatively uncommon in eastern parts but important on the western coastal plains of the Pilbara (Figure 1). Information on spinifex management options available to the pastoralist or station manager is …

Modeling Nutrient And Plankton Processes In The California Coastal Transition Zone: 3. Lagrangian Drifters, J. R. Moisan, Eileen E. Hofmann

CCPO Publications

Two types of numerical Lagrangian drifter experiments were conducted, using a set of increasingly complex and sophisticated models, to investigate the processes associated with the plankton distributions in the California coastal transition zone (CTZ). The first experiment used a one-dimensional (1-D; vertical) time-dependent physical-bio-optical model, which contained a nine-component food web. Vertical velocities, along the track of simulated Lagrangian drifters, derived from a three-dimensional (3-D), primitive equation circulation model developed to simulate the flow observed within the CTZ; were used to parameterize the upwelling and downwelling processes. The second experiment used 880 simulated Lagrangian drifters from a 3-D primitive equation …

The Diet Of Oryzomys Palustris Based On Stomach Content Analysis, Shannon L. Wright

Biological Sciences Theses & Dissertations

Oryzomys palustris (Harlan) is unusual in that it is one of two carnivorous mammals in the Family Muridae in North America. A diet analysis of Oryzomys palustris. the marsh rice rat, was done based on stomach contents. This was accomplished by taking the animals from two study areas located on the Eastern Shore of Virginia. The animals were taken monthly for one year. Once caught, the animals were sacrificed and their stomach contents were identified to the lowest taxonomic level possible. This study showed that rice rats were omnivorous mammals, eating a variety of foods, including dicots, monocots, crabs, …

Brown-Headed Cowbird (Molothrus Ater) Range Expansion Into The Southeast: Effects On The Passerine Populations Using North American Breeding Bird Survey Data, Elizabeth Pruitt Henderson

Biological Sciences Theses & Dissertations

North American Breeding Bird Survey data that were collected from 1971-1992 in Virginia, North Carolina, south Carolina, Georgia, Florida, Alabama and Tennessee were used in this analysis of the impact of Brown-headed Cowbird range expansion on four warbler and four vireo host species.

Maps of mean cowbird abundances were produced using kriging for the periods 1971-1975, 1976-1980, 1981-1985, 1986-1990 and 1991-1992. These maps illustrated a complex invasion of the southeastern United States by Brown-headed Cowbird.

Linear regression analyses of host and cowbird abundances within physiographic strata, showed no species to be in decline or increasing in abundance over the entire …

Hemorrhagic Gastritis In Free-Living Rodents In Idaho, Patricia G. Wilber, Donald W. Duszynski, B. Van Horne

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Between February 1992 and March 1994, four species of rodent from the Snake River Birds of Prey Area near Boise, Idaho (USA) were necropsied. Hemorrhagic gastritis was observed in 16 of 131 Townsend’s ground squirrels (Spermophilus townsendii), one of 11 Ord’s kangaroo rats (Dipodomys ordii) and the one Great Basin pocket mouse (Perognathus parvus) evaluated. No lesions were observed in 14 white-footed deer mice (Peromyscus maniculatus). Tissue from one Townsend’s ground squirrel was negative for Helicobacter sp.-like bacteria.

Characterization Of P39, A Borrelia Burgdorferi Specific Protein, Jill M. Troyer

Theses and Dissertations in Biomedical Sciences

Borrelia burgdorferi, the causative agent of Lyme disease, expresses a species specific, 39 kiloDalton protein of unknown function called P39. This protein is highly immunogenic and the presence of anti-P39 antibodies in patient sera is used as an indicator of B. burgdorferi infection. P39 is also a candidate for vaccine developement due to conserved expression of P39 among B. burgdorferi isolates. Since little is presently known about this important molecule, the present study was designed to characterize P39 and was carried out in six separate investigations. First, infectious and noninfectious isolates of B. burgdorferi were established in vitro, and …

Secondary Electron Emission From Simple Metals: Comparative Studies For Al, Mg, And Be, M. Rosler

Scanning Microscopy

In the secondary electron emission (SEE) from solids, the role of different excitation processes is now as ever of special interest from both the theoretical and the experimental points of view. Depending on the primary energy, the relative importance of different excitation mechanisms related to conduction as well as core electrons will be discussed for different simple metals. So far, first principles results are available only for Al for primary energies up to 10 keV. Starting from a microscopic description of the SEE based on the transport equation formalism, calculations were performed for other nearly-free-electron metals (Mg, Be) up to …

Spiranthes Cernua (L.) Rich., William Davison

Specimens by Name

No abstract provided.

Proceedings Of The International Conference On Genetic Improvement Of Sorghum And Pearl Millet, Darrell Rosenow, Gary Peterson, John Mullet, Henry Nguyen, Gebisa Ejeta, John Axtell, David Andrews, Jeff Dahlberg, A. Bruce Maunder, Kay Porter, Mike Gilbert, John Witcombe, John Stenhouse, C. Tom Hash, Kanayo Nwanze, Sam Mukuru, Anand Kumar, Francisco Gomez, Aboubacar Toure, Ouendeba Botorou

INTSORMIL Impacts and Bulletins

In 1971, an international symposium, "Sorghum in the Seventies", organized by the All India Coordinated Sorghum Improvement Project with support from the Indian Council of Agricultural Research and the Rockefeller Foundation was held in Hyderabad, India. The symposium reviewed the current knowledge base of the scientific, production and nutritional aspects of sorghum as a crop and as a human food. In 1981, ICRISAT, INTSORMIL, and the Indian Council of Agricultural Research (ICAR) sponsored "Sorghum in the Eighties", an international symposium at ICRISAT Center in India, to review the achievements accomplished in sorghum research during the preceding 10 years. They reviewed …

Hexapod Herald - Vol. 8, No. 13, September 27, 1996

Hexapod Herald and Other Entomology Department Newsletters

Grants

Congratulations

Office news

Welcome

Calendar of events

Deadlines

Deposition Of Supercoiled Dna On Mica For Scanning Force Microscopy Imaging, B. Samori, I. Muzzalupo, G. Zuccheri

Scanning Microscopy

The deposition of DNA molecules on mica is driven and controlled by the ionic densities around DNA and close to the surface of the substrate. Dramatic improvements in the efficiency and reproducibility of DNA depositions were due to the introduction of divalent cations in the deposition solutions. The ionic distributions on DNA and on mica determine the mobility of adsorbed DNA molecules, thus letting them assume thermodynamically equilibrated conformations, or alternatively trapping them in non-equilibrated conformations upon adsorption.

With these prerequisites, mica does not seem like an inert substrate for DNA deposition for microscopy, and its properties greatly affect the …

Simultaneous Identification Of A Specific Gene Protein Product And Transcript Using Combined Immunocytochemistry And In Situ Hybridization With Non-Radioactive Probes, Gwen V. Childs

Scanning Microscopy

Simultaneous identification of messenger RNA (mRNA) and proteins in the same cells or tissues is a valuable tool to help the cell biologist evaluate the cell secretory cycle. Some cells may produce the mRNA and delay the production of the proteins. Alternatively, the proteins may be rapidly secreted. Other cells may produce both in sequence within the same time frame. Because of this difference, some cells can only be identified by their mRNA product. Others may have both products. This presentation describes a non-radioactive approach to the detection of both products with dual-peroxidase labeling protocols in use in this laboratory …

Electrical Characterization Of Submicrometer Silicon Devices By Cross-Sectional Contact Mode Atomic Force Microscopy, P. De Wolf, T. Trenkler, T. Clarysse, M. Caymax, W. Vandervorst, J. J. Snauwaert, L. Hellemans

Scanning Microscopy

Two contact mode atomic force microscopic (AFM) techniques under ambient conditions are presented for the electrical evaluation of cross sectioned silicon devices. In the first technique, a conductive AFM tip is used as a voltage probe to determine the local potential distribution on the cross section of a silicon device under operation. The electrical potential is measured simultaneously with the surface topography with nanometer resolution and mV accuracy, offering an easy way of correlating topographic and electrical features. A second method, nanometer spreading resistance profiling (nano-SRP), performs localized spreading resistance measurements to determine the spatial distribution of charge carriers in …

Fluorescence Imaging And Spectroscopy Of Biomaterials In Air And Liquid By Scanning Near-Field Optical/Atomic Force Microscopy, H. Muramatsu, N. Chiba, K. Nakajima, T. Ataka, M. Fujihira, J. Hitomi, T. Ushiki

Scanning Microscopy

We have developed scanning near-field optical/atomic force microscopy (SNOM/AFM). The SNOM/AFM uses a bent optical fiber simultaneously as a dynamic force AFM cantilever and a SNOM probe. Resonant frequency of the optical fiber cantilever is 15-40 kHz. Optical resolution of the SNOM/AFM images shows less than 50 nm. The SNOM/ AFM system contains photon counting system and polychrometer/intensified coupled charge devise (ICCD) system to observe fluorescence image and spectrograph of micro areas, respectively. Cultured cells were stained with fluorescein isothiocyanate (FITC)-labeled anti-keratin antibody or FITC-labeled phalloidin after treatment with Triton X-100. Fluorescence and topographic images were obtained in air and …

Nucleic Acid Detection By In Situ Molecular Immunogold Labeling Procedures, Marc Thiry

Scanning Microscopy

We have recently combined immunogold labeling procedures with molecular biology methods to pinpoint the precise locations of nucleic acids in biological material at the ultrastructural level. These new immunocytological approaches involve the incorporation of labeled nucleotides in the nucleic acids present at the surface of ultrathin sections prior to immunogold labeling. The antibodies used recognize a nucleoside analogue (bromodeoxyuridine) or a hapten (biotin) employed to label nucleotides. Examples of high-resolution detection include DNA or RNA present in different substructures of cell nuclei, and in particular, in adenovirus-induced intranuclear regions of HeLa cells. In addition to being highly sensitive and specific, …

Freeze-Dried Human Leukocytes Stabilized With Uranyl Acetate During Low Temperature Embedding Or With Oso4 Vapor After Embedding, L. Edelmann, A. Ruf

Scanning Microscopy

Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO₄ vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.