Life Sciences Commons^™

Explorations, Vol. 1, No. 1, R. D. Blake, Katherine Carter, David Mackinnon Ebitz, Carole J. Bombard, Stephen A. Norton

Explorations — A Journal of Research

Welcome to the first issue of Explorations, A Journal of Research at the University of Maine at Orono.

Join us as we explore a representative selection of the pure and applied research of our faculty. At UMO, we believe that research can capture the imagination and invigorate the mind, as well as contribute directly to the quality of life of the citizens we serve.

In this first issue, we have selected four areas of research that span the disciplines of biological and environmental sciences and the arts. This is but a small part of the research conducted by the faculty …

Isolation Of A Collagenase Cdna Clone And Measurement Of Changing Collagenase Mrna Levels During Induction In Rabbit Synovial Fibroblasts., Robert H. Gross, Lynn A. Sheldon, Colin F. Fletcher, Constance E. Brinckerhoff

Dartmouth Scholarship

To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts, we have constructed a cDNA library using mRNAs isolated from cells induced with crystals of monosodium urate monohydrate. We have screened this library with cDNA probes made from induced and control mRNA populations. From among 30 clones that hybridized preferentially to the induced-cell probe, 4 contained collagenase sequences. The largest, a clone of 650 base pairs, was identified by its ability to hybrid select a mRNA that could be translated in a cell-free system into a product that was precipitable with monospecific …