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Effect Of Freezing On The Hydrocyanic Acid Potential Of Field-Grown Sorghum Tillers, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Effect Of Freezing On The Hydrocyanic Acid Potential Of Field-Grown Sorghum Tillers, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Department of Agronomy and Horticulture: Faculty Publications
Tissue disruption caused by freezing and thawing may contribute to rapid enzymatic breakdown of dhurrin [p-hydroxy-(S)mandelonitrile-β-D-glucoside], the cyanogenic glucoside of sorghum [Sorghum bicolor (L.) Moench], but published reports are not in agreement as to the effects of freezing on the hydrocyanic acid potential (HCN-p) of field-grown sorghum leaves. These effects were investigated in a series of experiments in the fan of 1983, a period during which weather conditions at Lincoln, NE, permitted repeated sampling of frozen and non-frozen leaves of field-grown sorghum tillers. Assays of these samples, both spectrophotometrically and with the cyanide electrode, indicated …
Influence Of Sample Treatment On Apparent Hydrocyanic Acid Potential Of Sorghum Leaf Tissue, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Influence Of Sample Treatment On Apparent Hydrocyanic Acid Potential Of Sorghum Leaf Tissue, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Department of Agronomy and Horticulture: Faculty Publications
When dhurrin [p-hydroxy-(S)-mandelonitrile-β-D-glucoside], the cyanogenic glucoside of sorghum [Sorghum bicolor (L.) Moench], is hydrolyzed by autoclaving, p-hydroxybenzaldehyde (p-HB) is released. The spectrophotometric determination of p-HB concentration in autoclaved sorghum leaf extracts provides a measure of the hydrocyanic acid potential (HCN-p) of leaf tissue. Extracts of field-grown sorghum leaves contained substances that interfered with this procedure, but ether extraction effectively separated p-HB from these interfering materials. We observed that when flag leaf tissue from field-grown sorghum was dried at 75°C and then autoclaved, HCN-p values were about three times as high …