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Progress In Scanning Electron Microscopy Of Frozen-Hydrated Biological Specimens, René Hermann, Martin Müller
Progress In Scanning Electron Microscopy Of Frozen-Hydrated Biological Specimens, René Hermann, Martin Müller
Scanning Microscopy
Modem scanning electron microscopy yields structural information down to 2 to 5 nm from thin, beam transparent biological specimens. This paper examines the possibilities of garnering this level of structural information from bulk, frozen-hydrated samples.
Freeze-fractured, frozen-hydrated yeast cells, frequently taken as a yardstick to monitor progress in low-temperature scanning electron microscopy, have been used to optimize both metal shadowing methods and observation parameters (e.g. accelerating voltage, electron beam irradiation of the specimen).
Uncoated frozen-hydrated yeast cells do not charge electrically at an accelerating voltage of 30 kV. Increasing charging effects are however observed with decreasing accelerating voltages. Very thin …