Life Sciences Commons^™

Influence Of Lipolytic Enzymes On The Fractional Composition Of Wheat Alcohol, Bobir S. Kayumov, Saida Sh. Khakimova, Khasan T. Khasanov

CHEMISTRY AND CHEMICAL ENGINEERING

The purpose of this work is to study the influence of lipolytic enzymes on the fermentation activity and fractional composition of alcohol by the yeast Saccharamyces cerevisea during alcoholic fermentation. Lipolytic enzymes, intensifying the fermentation process and increasing the yield of the target product, have a positive effect on the physiological activity of yeast cells. Hydrolysis of carboxylic acid esters also occurs due to the hydrolase activity of lipases. In the presence of exogenous lipases, hydrolysis of lipids occurs due to the hydrolase activity of lipases and the fractional composition of alcohol changes. The content of esters is reduced by …

Ty4 Retrotransposon Presence In Yeast Strains, Maria P. Walters, Eric T. Gillock

SACAD: John Heinrichs Scholarly and Creative Activity Days

Yeast is used in everyday life. It is important to understand the genetic components of yeast. In various laboratory yeast strains, there are findings of TY1, TY2, and TY3. TY1 through TY3 have been well studied, while TY4 and TY5 are retroelements that have yet to be thoroughly studied. It is known that TY4 is found at only one to three copies per haploid genome.

TY4 is a retrotransposon, which shares similarities with retroviruses, making it essential to study its presence in

yeast. This study aims to evaluate the prevalence of TY4 among lab strains, brewing strains, and baking strains …

Evaluating Microbial Contamination Of Bakeries, Wheat Flour In Ghaemshahr, Iran, Reyhaneh Gouran, Nazanin Khakipour

Journal of Bioresource Management

Although flour is regarded as a safe product from the perspective of microbiology due to its low water activity, it can be a suitable environment for the growth of microorganisms if suitable conditions are provided for their growth and reproduction. The objective of this study was to examine the microbial quality of 82 % and 78 % flours distributed in 5 bakeries in the city of Ghaemshahr. The evaluation of the results of the physicochemical tests on the samples revealed that sample No.5's moisture content (82 % of the flour sample from the bakery E) was significantly higher than other …

Comparison Of Fermentation Effects Of Six Kinds Of Probiotics On Rice Wine, Huang Zhi-Guo, Pu Ling-Ping, Ren Zhi-Qiang, Cheng Guo-Fu, Deng Jie

Food and Machinery

Objective: This study aimed to investigate the effects of 6 kinds of probiotics and their different adding ways on the production of rice wine. Methods: The probiotic rice wine was prepared by adding Lactobacillus rhamnosus BV-77, Lactobacillus fermentans TF-331, Lactobacillus saliva AP-32, Lactobacillus johnsoni MH-68, Lactobacillus acidophilus TYCA-06 and Lactobacillus casei CS-773. To compare the effects of saccharifying enzyme alone, saccharifying enzyme and yeast at the same time and adding methods （probiotic bran koji and probiotic powder） on alcohol content, reducing sugar, total acid and sensory quality of rice wine. Results: Yeast can promote the fermentation of probiotic rice wine …

Every Part Of Yeast Is The Best Part, Matthieu Baulez, Bruno Bertaud, Lisa Saibi

Proceedings of the Arkansas Nutrition Conference

Yeasts are single-cell, eukaryotic microorganisms classified in the fungi kingdom. They are generally around 10μm in size, have a nuclear membrane, a cell wall, and a cytoplasmic content. Yeasts are characterized as heterotrophs, which means they rely on organic material as sources of energy and nutrients. Not all yeasts are equal. There are about 60 genera and about 1,500 species of yeasts. Only a few are used commercially. Thanks to its exceptional fermentative capacities and nutritional properties, Saccharomyces cerevisiae is the main yeast used in food, beverage and baking as well as in animal nutrition. The main types of yeasts …

Evaluation Of Agrimune Liquid On Growth Performance Of Commercial Nursery Pigs, Andres F. Tolosa, Joel M. Derouchey, Jason C. Woodworth, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt

Kansas Agricultural Experiment Station Research Reports

A total of 1,188 pigs (L337 × 1050; PIC; initial BW of 11.1 lb) were used in a 40-d trial to determine the effect of a combination of mannan oligosaccharides, yeast culture, and beta-glucans (AgriMune Liquid; AFI-Agri Feed International, L.L.C., Rockwell, IA) on growth performance, morbidity, and mortality of pigs during the nursery period. Pigs were weaned at approximately 21 d of age and placed in pens (27 pigs per pen) based on initial BW. Pens were randomly assigned to 1 of 2 treatments in a randomized complete block design with 22 pens (replications) per treatment. Treatments consisted of the …

The Effect Of Live Yeast And Yeast Extracts On Growth Performance Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 340 weaned pigs (241 × 600, DNA; initially 11.2 lb BW) were used in a 45-d study to evaluate previous sow treatment (control vs. yeast additives) and nursery diets with or without added yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) on growth performance. At placement in the nursery, pigs were housed by pen based on previous sow treatment and randomly assigned to 1 of 2 dietary treatments with 5 pigs per pen and 17 replications per treatment. Treatments were arranged in a 2 × 2 factorial with main effects of sow treatment (control vs. yeast-based …

The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Sow And Litter Performance, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller, Brian Hotze

Kansas Agricultural Experiment Station Research Reports

A total of 80 sows (Line 241; DNA Genetics) across three farrowing groups were used in a study to evaluate the effect of feeding live yeast and yeast extracts to lactating sows on sow and litter performance. Sows were blocked by BW and parity on d 110 of gestation and allotted to 1 of 2 dietary treatments. Dietary treatments consisted of a standard corn-soybean meal lactation diet or a diet that contained yeast-based preand probiotics (0.10% Actisaf Sc 47 HR+ and 0.025% SafMannan; Phileo by Lesaffre, Milwaukee, WI). Diets were fed from d 110 of gestation until weaning (approximately d …

Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Nursery Pig Growth Performance And Fecal Consistency, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Hilda I. Calderón, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 360 weanling barrows (Line 200 × 400, DNA Genetics; initial BW 12.4 ± 0.05 lb) were used in a 42-d study to evaluate yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on growth performance and fecal dry matter (DM). Pens were assigned to 1 of 4 dietary treatments with 5 pigs per pen and 18 pens per treatment. Dietary treatments were arranged in a 2 × 2 factorial with main effects of live yeast-based pre- and probiotics (none vs. 0.10% ActiSaf Sc 47 HR+, 0.05% SafMannan, and …

Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Antimicrobial Susceptibilities Of Fecal Escherichia Coli In Nursery Pigs, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Qing Kang, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 360 weanling barrows (Line 200 × 400, DNA Genetics; initial BW 12.4 ± 0.05 lb) were used in a 42-d study to evaluate yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on antimicrobial resistance (AMR) patterns of fecalEscherichia coli. Pens were assigned to 1 of 4 dietary treatments with 5 pigs per pen and 18 pens per treatment. Dietary treatments were arranged in a 2 × 2 factorial with main effects of live yeast-based pre- and probiotics (none vs. 0.10% ActiSafSc 47 HR+, 0.05% SafMannan, …

The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Antimicrobial Susceptibility Of Fecal Escherichia Coli In Sows, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller, Brian Hotze

Kansas Agricultural Experiment Station Research Reports

A total of 27 sows (Line 241; DNA Genetics) were used in a study to evaluate the effect of feeding live yeast and yeast extracts to lactating sows on antimicrobial susceptibilities of fecalE. coli. Sows were blocked by BW and parity on d 110 of gestation and allotted to 1 of 2 dietary treatments. Dietary treatments consisted of a standard corn-soybean meal lactation diet or a diet that contained yeast-based pre- and probiotics (0.10% Actisaf Sc 47 HR+ and 0.025% SafMannan; Phileo by Lesaffre, Milwaukee, WI). Diets were fed from d 110 of gestation until weaning (approximately d …

The Effect Of Live Yeast And Yeast Extracts On Antimicrobial Susceptibilities Of Fecal Escherichia Coli Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, R. G. Amachawadi, V. Ishengoma, T. G. Nagaraja, M. D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 340 weaned pigs (Line 241 × 600, DNA; initially 11.2 lb BW) were used in a 45-d study to evaluate previous sow treatment (control vs. yeast additives) and nursery diets with or without added yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) on antimicrobial resistance (AMR) patterns of fecalEscherichia coli. At placement in the nursery, pigs were housed by pen based on sow treatment and randomly assigned to 1 of 2 dietary treatments with 5 pigs per pen and 17 pens per treatment. Treatments were arranged in a 2 × 2 factorial with main …

Innate Pathogenic Traits In Oral Yeasts, Ayat Ibrahiem Esmaeel Al-Laaeiby, Adnan A. Al-Mousawi, Inaam M.N. Alrubayae, Abdullah Al-Saadoon, Maysoon Almayahi

Karbala International Journal of Modern Science

The normal flora community plays a significant role in both healthy and patient individual’s bodies. It has improved its ability to change from beneficial to opportunistic organisms causing crucial infections in immunocompromised patients. This study was conducted to identify the community of yeast species, and to investigate potential virulence factors. Saliva samples were collected from healthy individuals, yeast species were isolated and identified using both traditional and advance molecular genetics technique. The results revealed that Candida albicans was the dominant isolate, while Meyerozyma caribbica was the least. The phylogenetic tree was constructed for 13 species using the Neighbour-Joining method, which …

Identification And Polar Lipid Composition Analysis Of Yeasts Isolated From Sufu Manufacture, Chen Si-Kai, Lin Sheng-Jie, Li Li

Food and Machinery

In order to characterize lipid metabolism of yeasts during sufu manufacture, 23 isolates from sufu pehtze and acidic tofu whey were identified. Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to analyse the lipid fingerprints of 7 strains of yeast, after the determination of lipase activities. It is suggested that lipid fingerprint mainly includes 2 kinds of sphingosine, 16 kinds of amide, 22 kinds of ester and 3 kinds of fatty acid. Kluyveromyces marxianus SP-1 and Candida (Pichia) norvegensis SP-5 show highest relative amount of 2-Amino-1,3,4-octadecanetrio and N, N-Dimethylsphingosine, which indicates potential for sphingolipids synthesis. Candida ethanolica ATW-1, Pichia …

How Cellular Metabolic State And The Chaperone Protein Hsp104 Interact To Affect The Spontaneous Formation Of The Yeast Prion [Ure3], Demyia Graham

Merge

No abstract provided.

Cereal Source And Microbial Consortia Of The Starter Culture Influence The Chemicalcomposition And Physicochemical Characteristics Of Boza, Mehli̇ka Borcakli, Tarik Öztürk, Erdem Yeşi̇lada

Turkish Journal of Agriculture and Forestry

Boza is a popular cereal-based traditional beverage particularly consumed during winter months in Balkan countries and Turkic societies. The present study aims to investigate the influence of different cereal sources and starter cultures used in the commercial production of boza on its chemical and microbiota compositions and physicochemical characteristics. Detailed experimentation has revealed that chemical compositions, i.e. carbohydrates (beta-glucans, starches), organic acids, vitamins, dietary fibers, proteins, fat, and minerals, along with physicochemical characteristics, are subject to significant differentiation depending upon the cereal types and microbial consortia of the starter culture used in boza production. These variations also influence the physicochemical …

Characterization Of The Beta1 Gene, Which Might Play A Role In Beta Vulgaris Subsp. Maritima Salt Tolerance, Özge Uysal, Çi̇ğdem Çakiroğlu, Ahmet Koç, Hüseyi̇n Çağlar Karakaya

Turkish Journal of Botany

Salinity stress has a negative impact on plant growth, which affects homeostasis and productivity. The uptake of nonessential salt ions changes the osmotic balance of the cell and causes dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. Sea beet (Beta vulgaris subsp. maritima) is a halophytic ancestor of cultivated sugar beet that displays salt stress tolerance. In this study, we screened a B. vulgaris subsp. maritima cDNA library in Saccharomyces cerevisiae strain Ab11c (ena1Δ, nha1/4Δ, nhx1Δ), which is deficient in sodium transport, to find sodium-detoxifying genes. We identified a cDNA construct, named BETA1, providing salt tolerance to yeast …

Screening Of Saccharomyces Cerevisiae Highly Producing Glutathione To Brew Cider With Healthcare Function, Huang Jinghan, Xu Jingran, Liu Aiping, Xu Xinbiao, Liu Shuangping, Mao Jian

Food and Machinery

The process of browning seriously influence the cider quality, but the glutathione producing by yeast can inhibit browning in the production and storage of cider. The glutathione (GSH) producing strain Saccharomyces cerevisiae Y18 was chosen as the starting strain, and after ultraviolet mutagenesis and diethyl sulfate mutagenesis, the strain highly producing GSH was screened by 96-well plates. Secondary screening was carried out by comparing fermenting power and GSH concentration after strain culture in shaker. The mutant strains Y18-20 GSH after screening by 96-well plates producing 46.8 mg/L GSH in the simulation of cider fermentation conditions, and it’s cell capacity of …

The Effect Of Live Saccharomyces Cerevisiae Yeast In The Diet Of Rams On The Digestibility Of Nutrients, Nitrogen And Mineral Retention, And Blood Serum Biochemical Parameters, Barbara Kowalik, Jacek Skomial, Renata Miltko, Malgorzata Majewska

Turkish Journal of Veterinary & Animal Sciences

The effect of live Saccharomyces cerevisiae yeast added to the diet of rams on the digestibility of carbohydrates and protein in the total tract, retention of nitrogen and mineral elements, and concentration of biochemical parameters in blood serum was examined. The experiment was carried out on 12 Corriedale rams allocated to 2 groups of 6 animals each. The control group was fed meadow hay (45.4%), concentrate (53.2%), and a vitamin?mineral mixture (1.4%), while the second group was fed the same diet supplemented with 2 g day-1 live S. cerevisiae. Adaptation to feeding lasted 21 days; the sampling period lasted 7 …

Evaluating The Effects Of Replacing Feed Grade Antibiotics With Yeast, Cinnamon, Or Zinc Oxide And Copper Sulfate On Nursery Pig Performance, A. Langemeier, J. Morton, S. Scotten, J. L. Nelssen

Kansas Agricultural Experiment Station Research Reports

A total of 288 weaned pigs (Line 200 × 400; DNA, Columbus, NE; initially 11.8 lb) were used in a 42-d study to compare the effects of feeding antibiotic alternatives (pharmacological trace minerals, copper and zinc, yeast, or essential oils), alone or in combination, on nursery pig performance in replacement to a common antimicrobial agent (carbadox, Mecadox®, Phibro Animal Health, Teaneck, NJ). Pigs were allotted to 1 of 9 dietary treatments in pens of 4 at weaning in a randomized complete block design with 8 replications per treatment. Dietary treatments were arranged with a negative control diet with no medication …

S. Cerevisiae $\Beta$-Glucan Reduced Viability Of Mouse Hepatoma Cells In Vitro, Artur Javmen, Ausra Nemeikaite-Ceniene, Saulius Grigiskis, Irena Jonauskiene, Mark Rudenkov, Darius Kacianauskas, Mykolas Mauricas

Turkish Journal of Biology

$\beta$-Glucan is a natural polymer, which is widely studied due to its multiple immunomodulatory properties. In addition, recent findings indicate potent antitumor properties of $\beta$-glucan. Saccharomyces cerevisiae, baker's yeast, is one of the commonly used sources of $\beta$-1,3-glucan. The aim of this work was to investigate S. cerevisiae $\beta$-glucan immunomodulatory activity against cancer cells.In our experiments, BALB/c mice were fed with insoluble whole $\beta$-glucan particles, and then their blood was collected for experiments. MH-22a hepatoma cells were treated with the blood of mice fed with $\beta$-glucan, and tumor cell viability was investigated after the treatment.The obtained results demonstrated that leukocytes …

A World Review Of Fungi, Yeasts, And Slime Molds In Caves, Karen J. Vanderwolf, David Malloch, Donald F. Mcalpine, Graham J. Forbes

International Journal of Speleology

We provide a review of fungi, yeasts, and slime molds that have been found in natural solution caves and mines worldwide. Such habitats provide frequent roost sites for bats, and in eastern North America the environmental conditions that support white-nose syndrome, a lethal fungal disease currently devastating bat populations. A list of 1029 species of fungi, slime moulds, and yeasts in 518 genera have been documented from caves and mines worldwide in 225 articles. Ascomycota dominate the cave environment. Most research has been conducted in temperate climates, especially in Europe. A mean of 17.9±24.4SD fungal species are reported per study. …

Anti-Oxidative Stress Activity Of Phikud Navakot Extract In Saccharomyces Cerevisiae, Nongluksna Sriubolmas, Uthai Sotanaphun, Duangdeun Meksuriyen, Suthep Wiyakrutta

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Analysis Of The Effects Of Chromatin Modifying Complexes On The Transcription Of Retrotransposon Ty2-917 In Saccharomyces Cerevisiae, Sezai̇ Türkel, Bükay Yeni̇ce

Turkish Journal of Biology

Transcription of the yeast retrotransposon Ty2-917 is regulated by a complex set of regulatory regions located both upstream and downstream of the transcription initiation site. In this study, it was found that certain chromatin modifying factors are essential for the transcriptional regulation of Ty2-917. Among the chromatin binding factors tested in this research, non-histone proteins Nhp6A/B have the largest effect on the transcription of Ty2-917. In Dnhp6A/B double mutant yeast strain transcription of Ty2 decreased 20-fold, indicating that these non-histone proteins are essential for the transcription of Ty2-917. Furthermore, it was also shown that the transcription of Ty2-917 decreased by …

Evidence For The Presence Of A Second Electron Donor For The Cytoplasmic Thioredoxins In The Yeast Saccharomyces Cerevisiae, Ahmet Koç, Hüseyi̇n Ç. Karakaya, Ercan S. Ünlü

Turkish Journal of Biology

In yeast, the cytoplasmic thioredoxin system is composed of NADPH, thioredoxin reductase-1 (TRR1) and 2 thioredoxin genes (TRX1, TRX2). In this study, using yeast knockout mutants for TRR1, TRX1 and TRX2 genes, the role of the thioredoxin system in methionine sulfoxide reduction was investigated. Cells lacking both TRX1 and TRX2 genes simultaneously were not able to reduce methionine sulfoxides to methionine; however, mutants missing the TRR1 gene were able to reduce methionine sulfoxides to methionine, which showed that electrons could be transferred from NADPH to thioredoxins in the absence of TRR1. Similar results were observed for 3-phosphoadenosine 5-phosphosulfate reduction in …

The Use Of Ram Horn Hydrolysate As A Supplement For Glycerol Production By Yeast, Esabi̇ Başaran Kurbanoğlu, Namudar İzzet Kurbanoğlu

Turkish Journal of Biology

This study describes the interesting potential of ram horns to be used as a supplement for glycerol production in batch fermentation. The use of ram horn hydrolysate (RHH) as a supplement for glycerol production was investigated using baker’s yeast, Saccharomyces cerevisiae. For this purpose, RHH was first produced. The production and chemical composition of the RHH was as described in our previous study. The effects of different concentrations (1 to 10% v/v) of RHH on the production of glycerol were investigated, and 8% RHH was found to be optimal. The content of glycerol in the culture broth containing RHH (6.3 …

Progress In Scanning Electron Microscopy Of Frozen-Hydrated Biological Specimens, René Hermann, Martin Müller

Scanning Microscopy

Modem scanning electron microscopy yields structural information down to 2 to 5 nm from thin, beam transparent biological specimens. This paper examines the possibilities of garnering this level of structural information from bulk, frozen-hydrated samples.

Freeze-fractured, frozen-hydrated yeast cells, frequently taken as a yardstick to monitor progress in low-temperature scanning electron microscopy, have been used to optimize both metal shadowing methods and observation parameters (e.g. accelerating voltage, electron beam irradiation of the specimen).

Uncoated frozen-hydrated yeast cells do not charge electrically at an accelerating voltage of 30 kV. Increasing charging effects are however observed with decreasing accelerating voltages. Very thin …

Cryo-Preparation And Planar Magnetron Sputtering For Low Temperature Scanning Electron Microscopy, T. Müller, P. Walther, C. Scheidegger, R. Reichelt, S. Müller, R. Guggenheim

Scanning Microscopy

Cryo-preparation is a reliable technique for the structural investigation of food products in low temperature scanning electron microscopy (SEM). Artifacts, such as, the segregation of water/non-water ingredients, occur during the freezing process by the crystallization of ice; they can be helpful for correct interpretation of visualized details, e.g., the detection of water containing compartments. The size of the segregation structures depends on water concentration and specimen thickness. The condensation of water vapor (ice contamination) is influenced by the specimen temperature and the partial pressure of the water inside the vacuum system. Furthermore, the evaporation (sublimation, etching) of specimen water can …

Intracellular Localization Of Heavy Metals In Yeast By X-Ray Microanalysis, Ljerka Kunst, Godfried M. Roomans

Scanning Electron Microscopy

The intracellular localization of heavy metals in yeast cells was studied by means of energy-dispersive X-ray microanalysis. The yeast, Saccharomyces cerevisiae, was pretreated with phosphate and then loaded with different metal ions, by suspending the cells in salt solutions (Ni, Zn, Cd, Pb, Al and Cr). For the analysis, the cells were embedded in gelatin, rapidly frozen, and thin cryosections were cut on a dry knife.

A considerable uptake of divalent cations by the yeast cells was found to occur. The cations were bound to the polyphosphate granules localized in or close to the cell vacuoles. Immediately after phosphate …

Use Of Peat Hydrolysate For Cultivation Of The Yeast, Candida Utilis, Jeffrey S. Denny, Allen G. Good

Journal of the Minnesota Academy of Science

No abstract provided.