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Full-Text Articles in Life Sciences

Diet Analysis Of Gastrotricha, Mikhail Anfinson Oct 2020

Diet Analysis Of Gastrotricha, Mikhail Anfinson

S.U.R.E Posters

The phylum Gastrotricha is comprised of small aquatic organisms that live in between sand grains. It has been shown that meiofauna communities can play valuable roles in ecosystems and serve as a food source for animals higher up the trophic levels. However, little is known about their own feeding behavior which this study is concerned about. Over the summer developments were made in the methodical sections for diet diagnostic PCR for marine bacterial strains, unicellular marine eukaryotes, and investigating different types of immunofluorescence for marine bacteria. We also plan at looking at the number of sarcomeres within the pharynx of …


Nutrient Limitation In Freshwater Streams In The South Carolina Piedmont, Rachael Rowe Oct 2020

Nutrient Limitation In Freshwater Streams In The South Carolina Piedmont, Rachael Rowe

S.U.R.E Posters

Availability of nutrients, particularly nitrogen (N) and phosphorus (P), is a key driver of microbial growth and metabolism in freshwater ecosystems. Microbial biofilms composed of both autotrophs and heterotrophs play crucial roles in freshwater food webs. These stream communities may be limited by N, P, or both N and P. This project examined nutrient limitation of biofilm communities in two small, freshwater streams in the Catawba river basin within the Catawba Nation in South Carolina. Stream conditions were monitored and nutrient concentrations for ambient ammonium and phosphate were monitored. We quantified nutrient limitation, community respiration, gross primary production, and ash-free …


The Effect Of Semaphorin 3a On Chick Embryo Retinal Growth Cones, Allison T. Reed Oct 2020

The Effect Of Semaphorin 3a On Chick Embryo Retinal Growth Cones, Allison T. Reed

S.U.R.E Posters

During embryonic development, axons grow from the retina of the eye to the tectum of the brain which allows for visual information transfer. Axons travel to the tectum via axon pathfinding, which is influenced by axon guidance cues. Axon guidance molecules interact with the growing tips of retinal ganglion cell (RGC) axon, which are motile structures known as growth cones. Some inhibitory axon guidance molecules are known to cause a growth cone to collapse, where they cease growth and then retract, or turn away. One such inhibitory axon guidance molecule is Semaphorin 3A (Sema 3A). While Sema 3A’s importance is …


Testing The Allelic Strength Of Drosophila Melanogaster Mus109 Alleles, Erica V. Nestore Oct 2020

Testing The Allelic Strength Of Drosophila Melanogaster Mus109 Alleles, Erica V. Nestore

S.U.R.E Posters

Drosophila melanogaster, commonly referred to as fruit flies, possess a group of genes that when mutated can cause sensitivity to DNA damaging agents. These mutagen sensitive (mus) genes are likely involved in DNA repair, and one of these genes, mus109, was the focus of this study. To perform the experiment, complementation crosses were set up between the three mus109 alleles: mus109lS, mus109D1 and mus109D2. The wild-type DGRP-59 was used as the control. For each cross, twenty vials were scored. Brood one contained ten vials that were mock treated with distilled water, and …


Investigating Cytotoxicity And Defense Functions Of Bacteriophage Larva Genes In Host Mycobacterium Smegmatis, Dallas K. Nivens, Laela A. Walker Oct 2020

Investigating Cytotoxicity And Defense Functions Of Bacteriophage Larva Genes In Host Mycobacterium Smegmatis, Dallas K. Nivens, Laela A. Walker

S.U.R.E Posters

It is estimated that approximately 75% of gene functions within any given phage cluster remain unknown. Two phage traits that can be measured phenotypically were investigated: cytotoxicity, which causes host cell lysis, and superimmunity, which results in protection of the host from infection by similar phages. Five genes (35, 42, 46, 49, and 59), from a subcluster K5 bacteriophage named Larva, were amplified by the polymerase chain reaction (PCR) and assembled into a cloning plasmid (pExTra) using isothermal assembly. Initially, chemical transformation of Escherichia coli with the pExTra + gene insert, enabled amplification of the plasmids. Clonal PCR …


Recellularization Of Porcine Internal Thoracic Arteries As A Tissue Engineered Small-Diameter Vascular Graft Alternative, Carlos E. Escoto-Diaz, Jesse B. Kooistra, Nicholle E. Lewis, Holdyn C. Ferguson Oct 2020

Recellularization Of Porcine Internal Thoracic Arteries As A Tissue Engineered Small-Diameter Vascular Graft Alternative, Carlos E. Escoto-Diaz, Jesse B. Kooistra, Nicholle E. Lewis, Holdyn C. Ferguson

S.U.R.E Posters

Small-diameter vascular grafts are the leading treatment for myocardial infarctions resulting from atherosclerosed coronary vasculature. A potential alternative to using patient-derived grafts is the use of tissue engineered vessels. The removal of native cells from xenogenic vessels allows for recellularization with human cells types; however, the decellularization process depends on the use of cytotoxic reagents that must be removed prior to recellularization. The recellularization process must produce a functional graft that contains the multiple cell types found within arteries. We hypothesized that porcine internal thoracic arteries decellularized using a combination of detergents could be processed to support human endothelial and …


Exploring The Mechanisms Regulating Toroid Formation By Adipose-Derived Stem Cells Cultured On 3d Collagen Hydrogels, Mary-Catherine Cobb Oct 2020

Exploring The Mechanisms Regulating Toroid Formation By Adipose-Derived Stem Cells Cultured On 3d Collagen Hydrogels, Mary-Catherine Cobb

S.U.R.E Posters

Mesenchymal stem cells (MSCs) are a huge topic of interest to many researchers due to their therapeutic potential. MSCs can be harvested from various body tissues, including bone marrow, blood, and adipose tissue. Adipose-derived mesenchymal stem cells (ADSCs) are of particular therapeutic interest due to their great abundance and ease of accessibility relative to other types of MSCs. We have observed that ADSCs will migrate and self-organize into a toroid when applied to the top of a 3D type I collagen hydrogel. ADSCs fail to form toroids when embedded within collagen hydrogels, which raises the question of what specific environmental …


Generation Of Fluorescent Esophageal Adenocarcinoma Cells For Lineage Tracing Within Composite 3d Culture Models, Madeline G. Linker Oct 2020

Generation Of Fluorescent Esophageal Adenocarcinoma Cells For Lineage Tracing Within Composite 3d Culture Models, Madeline G. Linker

S.U.R.E Posters

While relatively rare, the aggressive nature and poor prognosis associated with esophageal carcinoma make it a particularly dangerous form of cancer. One approach to discovering esophageal cancer treatment options includes the development of composite 3D culture models. This method of research allows cellular interactions and responses toward interventions to be evaluated within a more complex system than 2D cultures. Additionally, a composite 3D model provides a higher throughput and is more cost-effective than in vivo animal studies. To analyze the interactions between the model’s cell types, fluorescent labeling allows each cell type to be distinguished from the others, facilitating independent …


Knox-Wise Family Papers - Accession 591, Knox-Wise Jan 1985

Knox-Wise Family Papers - Accession 591, Knox-Wise

Manuscript Collection

The Knox-Wise Family Papers includes a land grant issued to John Knox in 1768; diaries written by Dr. John Knox [1792-1859] covering the 1840s and 1850s; James N. Knox [1806-1880] covering 1859-1880; and William D. Knox [1847-1928] covering 1869-1928; indentures, deeds, receipts, court summonses and other papers of Hugh Knox [1757-1821], sheriff and justice of the peace in Chester County, South Carolina (ca. 1780s and 1790s); correspondence of James N. Knox, correspondence, and other professional papers of Dr. John Knox; correspondence, and other papers of William D. Knox, Superintendent of Education in Chester County from 1896-1928. Papers of various other …


York County Extension Homemakers' Council Records - Accession 326, Extension Homemakers, York County Jan 1980

York County Extension Homemakers' Council Records - Accession 326, Extension Homemakers, York County

Manuscript Collection

This collection is a valuable source on home demonstration work in York County in the twentieth century. The inclusive dates for a particular series may vary and for most series the records are incomplete. The collection contains records of the organization including historical information, constitutions, correspondence, minutes, reports, program notes, photographs, newsletters, and other records.


Fleur-De-Lis Garden Club Records - Accession 163, Garden Club Of Rock Hill, Fleur-De-Lis Jan 1978

Fleur-De-Lis Garden Club Records - Accession 163, Garden Club Of Rock Hill, Fleur-De-Lis

Manuscript Collection

The Fleur-de-Lis Garden Club of Rock Hill Records consist of constitutions, bylaws, yearbooks, correspondence, program notes, photographs, and newspaper clippings, relating to the activities and history of the women’s garden club.


Garden Club Council Of South Carolina Records - Accession 173, Garden Club Council, South Carolina Jan 1978

Garden Club Council Of South Carolina Records - Accession 173, Garden Club Council, South Carolina

Manuscript Collection

The Garden Club Council of South Carolina Records consist of yearbooks, bulletins and other publications produced by the Garden Club Council of South Carolina which was founded on April 22, 1930. The Garden Club of South Carolina was federated with the National Council of State Garden Clubs in 1932, becoming the 25th state to join the national organization.


Boulware Family Papers - Accession 102, Thomas Mccullough Boulware, James Richmond Boulware Jan 1977

Boulware Family Papers - Accession 102, Thomas Mccullough Boulware, James Richmond Boulware

Manuscript Collection

The Boulware Family Papers consist of photocopies of a plantation journal (302 pages) kept by Thomas McCullough Boulware I (1829-1889) and a genealogy of the Boulware family written by James Richmond Boulware II of Lakeland, Florida, in 1948. The journal concerns the Blackstocks Plantation in Chester County, South Carolina and subjects include the planting of crops and farming of land; family events, such as deaths, births and marriages; family travel and vacations; church affairs, including the local temperance league; land sales; free black and northerners in Chester County after the Civil War.


South Carolina School Food Service Association - Accession 82, Food Service Association, South Carolina Jan 1977

South Carolina School Food Service Association - Accession 82, Food Service Association, South Carolina

Manuscript Collection

The South Carolina Food Service Association Records consist of historical statements, constitutions and by-laws, annual, officer and committee reports, minutes of the executive board, correspondence, financial records, membership records, newsletters, handbooks, photographs, newspaper clippings and other records. There are records relating to the regional and national school food service associations and to the 1963 International Congress on Home Economics.


Mary Johnston Gates Papers - Accession 110, Mary Johnston Gates Jan 1977

Mary Johnston Gates Papers - Accession 110, Mary Johnston Gates

Manuscript Collection

The Mary Johnston Gates Papers consist of correspondence, minutes, histories, financial records, program notes, newspaper clippings, photographs, scrapbooks, and reference material, relating to the South Carolina Extension Homemakers’ Council (1930-1976), Bethel Home Demonstration Club (1941-1975), the National Extension Homemakers’ Council (1948-1976), the Associated Country Women of the World (1968-1976) and the South Carolina Agricultural County Extension Work (1928-1968) . There is also a 16mm film describing the dedication ceremonies of the Bethel Home Demonstration Club of Sumter, South Carolina, the first of its kind in the United States. Correspondents include Strom Thurmond, US Senator from South Carolina; Donald Russell, former …


Farmer's Almanack 1825, Robert B. Thomas Dec 1824

Farmer's Almanack 1825, Robert B. Thomas

Rare Books

The Farmer's Almanack contains astronomical calculations, a farmer's calendar, military fines, college vacations, table of simple interest, court calendar, agricultural information, Episcopalian holidays, poetry, anecdotes, weather observations, roads and innkeepers and other useful information.