Life Sciences Commons^™

Effects Of Different Land-Use Types On The Space Use Of The Woodland Box Turtle, Sydney Grant

Graduate Theses

Human land use change has resulted in extensive habitat loss, degradation, and fragmentation, threatening biodiversity worldwide. The population of woodland box turtles (Terrapene carolina carolina) has declined because of land use changes throughout the temperate broadleaf forests of the eastern United States. However, the responses of woodland box turtles to land use change remain unclear, with inconsistent responses documented in the literature. To understand how woodland box turtles respond to different land cover types, this study estimated habitat selection and home range in a mosaic landscape in the Piedmont of South Carolina, U.S.A. Specifically, this study asked whether woodland box …

The Contribution Of Micrornas To Rybp Silencing In Glioblastoma Multiforme, Alex B. Lee

Graduate Theses

Glioblastoma multiforme (GBM) is a highly aggressive and invasive tumor of the central nervous system (CNS). Survival rates are abysmal, with only 7.2% of patients alive 5-years after diagnosis. Because of this, understanding epigenetic alterations that give GBM tumors their aggressive phenotypes is critical for the development of more targeted and effective therapies. These alterations frequently affect a group of proteins called the Polycomb group proteins, which play important oncogenic and tumor suppressive roles in cancer. One Polycomb protein, the RING1- and YY1-binding protein (RYBP), is downregulated in a majority of GBM patients, suggesting a strong tumor suppressive property. In …

Impacts Of Urbanization On Demographics And The Stress Level Of White-Tailed Deer In The Piedmont Of South Carolina, Cameron Bramhall

Graduate Theses

Population demographics and density studies are a common way of determining number of individuals in location. Understanding how many individuals and sex ratios can help estimate total populations for future potential management practices. For white-tailed deer (Odocoileus virginianus), commonly referred to as whitetail deer, like other cervids, having too many or too few individuals in an area can cause issues. Some of the issues that may arise with too many individuals include higher disease potential, and a general lack of resources leading to an increase in competition. As areas that were once habitat for whitetail deer become more and more …

Threads Of Connection: An Offering To Re-Tangle Humanity And Nature With The Patterns Of Our World, Emily Shelton

Graduate Theses

In our world there are patterns of self-similarity that serve as evidence of the interconnectedness between humankind and the rest of the natural world. They are reflected in our bodies, behaviors, and environments, both natural and manmade, and can be found throughout systems at every scale, micro through macro. These organic, linear motifs branch into smaller iterations that seem to shape our existence on this planet as we gravitate towards experiences that echo these patterns. During everyday acts like shopping in a grocery store or a crowd at a concert, we unconsciously participate in self-similar collective movements as we navigate …

The Management Of Non-Ige-Mediated Allergies In Human Milk-Fed Infants, Sarah Kelly Rowe

Graduate Theses

Background: Non-IgE (immunoglobulin E)-mediated food allergies (FA) present diagnostic and management challenges despite guidelines due to knowledge gaps amongst clinicians. Given that 60% of food protein-induced allergic proctocolitis (FPIAP) cases occur in breast-fed infants, maternal elimination diets are routinely prescribed to manage allergy symptoms. The implications of maternal dietary eliminations extend beyond maternal nutrition to consideration of infant nutrition and feeding skill acquisition.

Methods: A survey-based retrospective, cross-sectional study sought to identify dietary triggers and symptom management techniques practiced by (n=59) lactating mothers of infants with confirmed and suspected cases of non-IgE FA in the United States. Statistical significance was …

Effects Of Nonnative Plants On Space-Use In Eastern Box Turtles, Emily Ilene Crago

Graduate Theses

Introduction of nonnative plants outside their natural range has caused widespread reduction in the abundance and diversity of native plant species. Nonnative plants typically form dense, often monotypic, thickets that affect the ability of animals to find food, reproduce, avoid predation risk, and thermoregulate. By doing so, nonnative plants have the potential to displace resident animals from areas otherwise suitable as animal habitat. However, limited studies have investigated whether resident animals avoid areas dominated by nonnative plants. This study investigated impacts of nonnative plants on eastern box turtles (Terrapene carolina carolina), a species that has been declining throughout their range. …

Investigating The Phenotypic Effects Of Ring1- And Yy1-Binding Protein (Rybp) In Glioblastoma Multiforme, Ronald W. Bucher

Graduate Theses

Glioblastoma multiforme (GBM) is an aggressive form of brain cancer that has horrendous survival outcomes with the use of current therapies. Further study into its molecular mechanisms will inform development of new, more effective treatments. The Polycomb protein RING1- and YY1- Binding Protein (RYBP) has emerged as an important gene in multiple cancers. In complex with other Polycomb proteins, RYBP acts to repress regions of chromatin, though it also performs other functions independent of these complexes. RYBP has a tumor suppressive role in various cancers, but may act as an oncogene in others, demonstrating its context-specific effects. The role of …

Effects Of Titanium Dioxide Nanoparticle Injections On Tissue Function And Morphology In Goldfish (Carassius Auratus), Bryan Joel Patterson

Graduate Theses

Fish gills are sensitive to a variety of environmental factors because they regulate the exchange of oxygen and ions between the fish and the external environment. This study examined the effects of polyacrylic acid (PAA) coated titanium dioxide nanoparticle injections (TiO₂ NPs) on goldfish (Carassius auratus) over 14 days. Following the experimental period, we examined gill histological samples and looked for signs of an inflammatory response through blood glucose levels and relevant gene expression. TiO₂ injected fish developed a more robust ILCM (interlamellar cell mass) compared to the non-injected controls, which remained elevated over 14 days post injection. …

Investigations Into Prg-2 And Its Involvement In Developing Gallus Gallus Retinal Neurons, Jeffrey Parham

Graduate Theses

I am interested in the development of the nervous system, especially since during development nerves grow and extend, but in adults, they do not regenerate if damaged. We are specifically interested in the molecules that guide nerves to the correct target during their development. Lysophosphatidic acid (LPA) is a bioactive molecule that has been shown to play a role in neural development. LPA, through repeated studies, has been shown to stop neurons from growing by causing a physical change in a neuron’s growth cone (a structure used for navigation and growth). Recently, a novel set of genes, called PRGs, have …

Full Issue, Winthrop Mcnair Research Bulletin

The Winthrop McNair Research Bulletin

Winthrop McNair Research Bulletin Volume 5, Full Issue

The Relationship Between Nature Connectedness And Physical Activity Patterns In A Sample Of Collegiate Students, Faculty, And Staff, Destinee Waddy

The Winthrop McNair Research Bulletin

PURPOSE: A strong relationship exists between physical activity (PA) and nature connectedness (NC); the most physically active individuals may also be the most nature connected. Designing PA programs and modifying college campuses through the lens of biophilia can provide a more logical, evidence-based approach to improve overall health and wellness. The purpose of this study was to identify the relationship between physical activity and nature connectedness in a sample of collegiate faculty and students. METHODS: Participants completed two previously validated surveys: The International Physical Activity Questionnaire and the Connectedness to Nature Survey. Demographic data (age, race, campus affiliation) were also …

Locating Mutagen-Sensitivity Gene Mus109 In The Drosophila Melanogaster Genome Using Deficiency Mapping, Chandani Mitchell

The Winthrop McNair Research Bulletin

The complex processes involved in repairing damaged DNA are still being elucidated. Some genes that are known to have roles in the DNA repair process have been identified, such as the mutagen-sensitivity genes, or mus genes, in Drosophila melanogaster. However, the precise genomic location of some mus genes is still unknown, including mus109. It is known that mutations in mus109 cause chromosomal aberrations resulting in larval death, and previous research has mapped mus109 to a region of the X chromosome consisting of over 520,000 nucleotides and 41 genes. Therefore, this study aimed to locate mus109 using deficiency mapping. The mus109D2 …

Mindful Eating And Perceived Stress In College Students, Emily G. Garrett

Graduate Theses

Background: Evidence suggests that in college student populations, stress levels often manifest as maladaptive eating and/or functional gastrointestinal disorders. These conditions can degrade wellbeing and academic performance if not addressed.

Research aim/question(s): The purpose of this study was to examine the relationships between students’ perceived stress, their overall mindfulness, and mindful eating patterns. Gastrointestinal (GI) symptoms and bowel habits were also investigated to determine whether these factors were stress-related or influenced mindfulness behaviors.

Materials and Methods: An online survey was conducted during the 2020-2021 academic year. Demographic information, including smoking, alcohol, and drug use patterns, was gathered in addition to …

Is The Dewlap An Honest Signal Of Fighting Ability In The Male Green Anole (Anolis Carolinensis)?, Alexia Hughes

Graduate Theses

Signals are morphological or behavioral traits that an individual uses to influence the behavior or actions of another. These signals can be used in male-male competition, in which male secondary sexual traits act as a signal of his fighting ability. Animal signals are considered honest when the signal reliably indicates a specific trait or condition of the individual. The genus Anolis, comprised of over 400 species that occupy the tropics and the southeastern United States, utilize aggressive signaling prior to physical combat. Research on several tropical species of anole indicates that the size of their dewlap can act as an …

Mapping And Characterization Of Mutagen Sensitivity Genes In Drosophila Malanogaster, Alexis Nystrom

Graduate Theses

The information contained within DNA is vital to directing all biological processes. All organisms have repair mechanisms in place to repair DNA damage quickly and efficiently. Without these repair pathways, DNA can acquire harmful mutations that can compromise the survival of an organism. Studies of DNA repair in Drosophila melanogaster have focused on mutagen sensitive (mus) mutants, each of which contain a mutation that renders them incapable of performing DNA repair. Since a majority of these mus genes are unmapped, the goal of this project was to determine what genes in the Drosophila melanogaster genome are mus106 and mus108. Presence …

Relationship Between Disordered Eating Risk & Body Image Dissatisfaction Of Spirit Squad Members, Sarah Abdul Albandar

Graduate Theses

Background: Investigations into body image and disordered eating habits among aesthetic sports has shown that these athletes face pressures to be thin and to be a certain body type for their sport.

Research aim/question(s): This study sought to determine if disordered eating habits and body dissatisfaction were prevalent within this population, whether or not there is a relationship between body image and disordered eating, and to determine the current research gap on body image and disordered eating among cheerleaders and dancers.

Materials and Methods: Participants were 23 females including cheerleaders and dancers on the Spirit Squad as well as dance …

The Occurrence Of Microplastics Among Freshwater Fish Guilds In A South Carolina Reservoir, Chasity Rae Moore

Graduate Theses

No abstract provided.

Diet Analysis Of Gastrotricha, Mikhail Anfinson

S.U.R.E Posters

The phylum Gastrotricha is comprised of small aquatic organisms that live in between sand grains. It has been shown that meiofauna communities can play valuable roles in ecosystems and serve as a food source for animals higher up the trophic levels. However, little is known about their own feeding behavior which this study is concerned about. Over the summer developments were made in the methodical sections for diet diagnostic PCR for marine bacterial strains, unicellular marine eukaryotes, and investigating different types of immunofluorescence for marine bacteria. We also plan at looking at the number of sarcomeres within the pharynx of …

Nutrient Limitation In Freshwater Streams In The South Carolina Piedmont, Rachael Rowe

S.U.R.E Posters

Availability of nutrients, particularly nitrogen (N) and phosphorus (P), is a key driver of microbial growth and metabolism in freshwater ecosystems. Microbial biofilms composed of both autotrophs and heterotrophs play crucial roles in freshwater food webs. These stream communities may be limited by N, P, or both N and P. This project examined nutrient limitation of biofilm communities in two small, freshwater streams in the Catawba river basin within the Catawba Nation in South Carolina. Stream conditions were monitored and nutrient concentrations for ambient ammonium and phosphate were monitored. We quantified nutrient limitation, community respiration, gross primary production, and ash-free …

The Effect Of Semaphorin 3a On Chick Embryo Retinal Growth Cones, Allison T. Reed

S.U.R.E Posters

During embryonic development, axons grow from the retina of the eye to the tectum of the brain which allows for visual information transfer. Axons travel to the tectum via axon pathfinding, which is influenced by axon guidance cues. Axon guidance molecules interact with the growing tips of retinal ganglion cell (RGC) axon, which are motile structures known as growth cones. Some inhibitory axon guidance molecules are known to cause a growth cone to collapse, where they cease growth and then retract, or turn away. One such inhibitory axon guidance molecule is Semaphorin 3A (Sema 3A). While Sema 3A’s importance is …

Testing The Allelic Strength Of Drosophila Melanogaster Mus109 Alleles, Erica V. Nestore

S.U.R.E Posters

Drosophila melanogaster, commonly referred to as fruit flies, possess a group of genes that when mutated can cause sensitivity to DNA damaging agents. These mutagen sensitive (mus) genes are likely involved in DNA repair, and one of these genes, mus109, was the focus of this study. To perform the experiment, complementation crosses were set up between the three mus109 alleles: mus109^lS, mus109^D1 and mus109^D2. The wild-type DGRP-59 was used as the control. For each cross, twenty vials were scored. Brood one contained ten vials that were mock treated with distilled water, and …

Investigating Cytotoxicity And Defense Functions Of Bacteriophage Larva Genes In Host Mycobacterium Smegmatis, Dallas K. Nivens, Laela A. Walker

S.U.R.E Posters

It is estimated that approximately 75% of gene functions within any given phage cluster remain unknown. Two phage traits that can be measured phenotypically were investigated: cytotoxicity, which causes host cell lysis, and superimmunity, which results in protection of the host from infection by similar phages. Five genes (35, 42, 46, 49, and 59), from a subcluster K5 bacteriophage named Larva, were amplified by the polymerase chain reaction (PCR) and assembled into a cloning plasmid (pExTra) using isothermal assembly. Initially, chemical transformation of Escherichia coli with the pExTra + gene insert, enabled amplification of the plasmids. Clonal PCR …

Recellularization Of Porcine Internal Thoracic Arteries As A Tissue Engineered Small-Diameter Vascular Graft Alternative, Carlos E. Escoto-Diaz, Jesse B. Kooistra, Nicholle E. Lewis, Holdyn C. Ferguson

S.U.R.E Posters

Small-diameter vascular grafts are the leading treatment for myocardial infarctions resulting from atherosclerosed coronary vasculature. A potential alternative to using patient-derived grafts is the use of tissue engineered vessels. The removal of native cells from xenogenic vessels allows for recellularization with human cells types; however, the decellularization process depends on the use of cytotoxic reagents that must be removed prior to recellularization. The recellularization process must produce a functional graft that contains the multiple cell types found within arteries. We hypothesized that porcine internal thoracic arteries decellularized using a combination of detergents could be processed to support human endothelial and …

Exploring The Mechanisms Regulating Toroid Formation By Adipose-Derived Stem Cells Cultured On 3d Collagen Hydrogels, Mary-Catherine Cobb

S.U.R.E Posters

Mesenchymal stem cells (MSCs) are a huge topic of interest to many researchers due to their therapeutic potential. MSCs can be harvested from various body tissues, including bone marrow, blood, and adipose tissue. Adipose-derived mesenchymal stem cells (ADSCs) are of particular therapeutic interest due to their great abundance and ease of accessibility relative to other types of MSCs. We have observed that ADSCs will migrate and self-organize into a toroid when applied to the top of a 3D type I collagen hydrogel. ADSCs fail to form toroids when embedded within collagen hydrogels, which raises the question of what specific environmental …

Generation Of Fluorescent Esophageal Adenocarcinoma Cells For Lineage Tracing Within Composite 3d Culture Models, Madeline G. Linker

S.U.R.E Posters

While relatively rare, the aggressive nature and poor prognosis associated with esophageal carcinoma make it a particularly dangerous form of cancer. One approach to discovering esophageal cancer treatment options includes the development of composite 3D culture models. This method of research allows cellular interactions and responses toward interventions to be evaluated within a more complex system than 2D cultures. Additionally, a composite 3D model provides a higher throughput and is more cost-effective than in vivo animal studies. To analyze the interactions between the model’s cell types, fluorescent labeling allows each cell type to be distinguished from the others, facilitating independent …

Optimization Of Dna Extraction From Human Milk, Cassandra Z. Rutherford

Graduate Theses

Background: Techniques are emerging for determining the best and most cost-effective way to test for human milk adulteration. Currently, the literature is focused on the use of qPCR testing, a technique used to isolate and amplify pieces of DNA for analysis. However, no recommendation currently exists on the best DNA extraction kit to use to achieve optimal DNA yield or purity from human milk samples for downstream qPCR use. Thus, the objective of this study is to assess and compare two DNA extraction kits for use with human milk samples for future DNA-based analysis in the testing for bovine milk …

Effects Of Pine Litter Raking On Plant Community Composition And Soil Seed Banks In Longleaf Pine Savannas, Jordan Andrew Winter

Graduate Theses

Longleaf pine (Pinus palustris) savannas have been reduced to a small percentage of their original range in the southeastern United States. These savannas are fire-reliant and require frequent, low-intensity fires to maintain understory plant diversity. Currently, many landowners rake pine litter off the forest floor of longleaf pine savannas for subsequent sale in horticulture. Though raking is a common practice, little is known about the effects of raking on the understory plant community, the soil seed bank, or fire intensity. I conducted my research in two longleaf pine savanna sites where raking has occurred. At the McCain Forest Management Area …

Development Of A Crispr System To Mutate Lpar4 In Chick Retinal Ganglion Cells, Garrett Driscoll

Graduate Theses

Growth cones direct axon pathfinding during neural development by detecting environmental stimuli, known as axon guidance molecules. In vitro studies have shown inhibitory axon guidance molecules to cause growth cones to change morphology to what is called a collapsed growth cone. Lysophosphatidic acid (LPA) has been demonstrated to collapse growth cones in culture, and thus it may act as an inhibitory molecule. However, identification of what LPA receptor is responsible for this physiological response is unknown. To investigate which receptor elicits collapse, we focused on LPA receptor-4 (LPA4) by designing a CRISPR construct to mutate Lpar4 in chick retinal ganglion …

A Comparison Of Coyote Diets In Urban And Rural Habitats In The Piedmont Of South Carolina, Bethany Krug

Graduate Theses

With increasing rates of urban expansion, interactions between humans and wildlife become inevitable. These urban environments present novel situations to native species, frequently resulting in their displacement or extirpation. However, some species, often referred to as “urban adapters”, have thrived in these landscapes. Coyotes (Canis latrans) are a prime example of a species that has adapted to exploit urban habitats. Coyotes are omnivores with food choices ranging from small/medium mammals to invertebrates depending on habitat. With their recent range expansion into the Southeast, little is known of their behavioral ecology in the region, especially details relating to their diet. Macroscopic …

The Effect Of Staphylococcus Aureus Exposure On White-Tailed Deer Trabecular Bone Stiffness And Yield, Emily Brooke Long

Graduate Theses

Osteomyelitis is an infection of the bone or bone marrow caused by the infiltration of bacteria, resulting in destructive inflammation, bone necrosis and abnormal bone remolding. With a growing number of osteomyelitis diagnoses, many of which are linked to Staphylococcus aureus (S. aureus), it is imperative to understand the pathology of S. aureus in relation to bone to provide better diagnostics and patient care. While the cellular mechanisms of S. aureus and osteomyelitis have been studied, little information exists on the biomechanical effects of such infections. We postulated that exposure to S. aureus for 72 hours would significantly decrease both …