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VIMS Articles

2017

Chemical Analysis; Near Infrared Reflectance Spectroscopy; Oyster; Crassostrea Virginica; Glycogen Concentration; Freeze-Dry

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Full-Text Articles in Life Sciences

Glycogen Concentration In Freeze-Dried Tissues Of Eastern Oyster (Crassostrea Virginica) Using Near Infrared Reflectance Spectroscopy To Determine The Relationship Between Concentrations Of The Tissues Excised For Histological Sampling And The Remaining Tissues, Eric Guevelou, Joseph L. Matt, Standish K. Allen Jr. Jan 2017

Glycogen Concentration In Freeze-Dried Tissues Of Eastern Oyster (Crassostrea Virginica) Using Near Infrared Reflectance Spectroscopy To Determine The Relationship Between Concentrations Of The Tissues Excised For Histological Sampling And The Remaining Tissues, Eric Guevelou, Joseph L. Matt, Standish K. Allen Jr.

VIMS Articles

To improve the accuracy and reproducibility of the previous near infrared reflectance spectroscopy (NIRS) model for glycogen in the oyster species Crassostrea virginica, a new model using freeze-dried samples was developed. The NIRS glycogen calibration model was developed using 380 individual oyster samples collected between 2014 and 2016 from several locations in the Chesapeake Bay. Homogenized freeze-dried samples were scanned in the near infrared region between 1,000 and 2,500 nm. In parallel, glycogen concentration (GC), measured as percent dry weight, was determined using laboratory-based methods. The two sets of data allowed us to build a NIRS model based on freeze-dried …