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Full-Text Articles in Life Sciences
Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard
Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard
Turkish Journal of Biology
Apoptosis induced by hydrogen peroxide (H_2O_2 ) was investigated in Chinese hamster ovarian (CHO) cells, and compared to apoptosis induced by cisplatin (25 µM), a well-established apoptosis-inducing agent. In preliminary experiments, CHO cells were treated with low concentrations of H_2O_2 (250-500 µM) continuously or for 3 hours, followed by washing and further incubation in complete growth medium for 1-10 days. Fluorescence microscopy of acridine orange-stained cells indicated morphological changes of apoptosis. Similar changes were observed in cisplatin-treated cells. DNA-laddering also revealed that DNA fragmentation occurred in both adherent and non-adherent cells by day 2. To prevent H_2O_2 -mediated apoptosis, the …
The Inhibition Effect Of Yoghurt Starter Culture Metabolites, Belma Aslim, Yavuz Beyatli, Kadi̇r Halkman
The Inhibition Effect Of Yoghurt Starter Culture Metabolites, Belma Aslim, Yavuz Beyatli, Kadi̇r Halkman
Turkish Journal of Biology
In this study effect of some separate and combined metabolic compounds produced by lactic acid were tested on food pathogenic and contaminant microorgnasms. For this study, five strains of each Lactobacillus bulgaricus and Streptococcus thermophilus were used. The inhibition effects of the metabolic products (lactic acid, hydrogen peroxide, acetaldehyde, diacetyl) produced by the stains on test bacteria ( Staphylococcus aureus, Escherichia coli) were determined. Also, a random combinations of the strains were prepared and the metabolic compounds produced by these cultures and their antimicrobial activities on tested bacteria were determined. By regarding the metabolic products mainly, the samples of lactic …