Life Sciences Commons^™

Analysis Of Clinically Important Compounds Using Electrophoretic Separation Techniques Coupled To Time-Of-Flight Mass Spectrometry, Zlatuse Durda Peterson

Theses and Dissertations

Capillary electrophoretic (CE) separations were successfully coupled to time-of-flight mass spectrometric (TOFMS) detection for the analysis of three families of biological compounds that act as mediators and/or indicators of disease, namely, catecholamines (dopamine, epinephrine, norepinephrine) and their O-methoxylated metabolites (3-methoxytyramine, norepinephrine, and normetanephrine), indolamines (serotonin, tryptophan, and 5-hydroxytryptophan), and angiotensin peptides. While electrophoretic separation techniques provided high separation efficiency, mass spectrometric detection afforded specificity unsurpassed by other types of detectors.

Both catecholamines and indolamines are present in body fluids at concentrations that make it possible for them to be determined by capillary zone electrophoresis coupled to TOFMS without employing any …

The Effect Of Wearing Mouthguards On Vo2, Ventilation, And Perceived Exertion At Two Different Exercise Intensities, Jeffrey Scott Hurst

Theses and Dissertations

Objective: To assess the effects of wearing a protective mouthguard during exercise on ventilation and oxygen consumption.
Design and Setting: All participants performed a graded maximal exercise test on a cycle ergometer to determine peak oxygen consumption (VO2peak). Each participant also performed 6 submaximal exercise tests while wearing one of two facemasks (nasal or non-nasal breathing) and one of three mouthguard conditions (no mouthguard, boil and bite, custom-fit). Steady-state VO2, rate of perceived exertion (RPE), and other ventilatory values were measured at 60% and 80% of VO2max during each submaximal exercise test. All 6 submaximal exercise tests were completed within …

Phylogeny Of The Polyneopterous Insects With Emphasis On Plecoptera: Molecular And Morpological Evidence, Matthew Dana Terry

Theses and Dissertations

Polyneoptera is an assemblage of eleven insect orders comprising the "orthopteroid" insects. It includes familiar insects such as grasshoppers, roaches, termites, earwigs and preying mantises; as well as the more obscure web-spinners, angel insects and ice-crawlers. We present a phylogenetic analysis of the polyneopteran orders based on 18S rDNA, 28S rDNA, Histone 3, and a coded morphology matrix for an extensive sampling of taxa. We investigate the use of congruence between separate datasets as an a priori measure of alignment quality. Our results support the paraphyly of Polyneoptera, the monophyly of Dictyoptera, sister taxon relationships between Embiidina + Phasmatodea and …

Effects Of A Tape Cast, Air-Stirrup, And An Air-Stirrup Applied Over A Taped Ankle On Dynamic Ankle Inversion, Justin K. Freeman

Theses and Dissertations

Objective: To compare the tape cast, and an Air-Stirrup/closed basketweave combination to the Air-Stirrup alone with respect to their effects on dynamic ankle inversion.

Design and Setting: This study used a repeated measures design with the independent variables being the exercise bout and the ankle support method (4 levels: no support, Air-Stirrup only, Air-Stirrup/closed basketweave, and tape cast). The 2 dependent variables were total inversion and maximum inversion velocity. Measurements were taken before and after an exercise bout, and all trials were conducted in the university's human performance laboratory.

Subjects: 16 subjects (11 male, 5 female, age 24.3 ± 1.8 …

Examination Of Adolescent Physical Activity And Overweight Levels , Maika Elizabeth Nelson

Theses and Dissertations

Research suggests declines in physical activity may occur after age 12. The purpose of this study was to determine the relationships between adolescent physical activity, BMI, and nutrition. Adolescents, ages 12-17 (N = 223), wore pedometers for 11 consecutive days and completed a nutrition survey, the Youth Adolescent Questionnaire. Activity levels did not differ among the ages of 12-17 year old adolescents, and boys were more active than girls. There was a low, but significant relationship between BMI and physical activity levels for both boys and girls. Energy intake was not related to BMI or physical activity levels in boys …

Phylogenetic Systematics And Evolution Within The Family Scincidae, Alison Swindle Whiting

Theses and Dissertations

Scincidae (skinks) comprise one of the largest families of lizards, and the more than 1300 species show great variation in body size and form, and are found worldwide in a diversity of habitats. The group presents many interesting questions ranging from the colonization of oceanic islands, to the evolution of limb loss, yet most of these and other questions remain understudied. The purpose of this dissertation is to use multiple mitochonidrial and nuclear DNA markers in connection with current cladistic methods to address evolutionary questions at many levels within Scincidae. In chapter one, a molecular phylogenetic study, based on six …

Determination Of Four Production Indicators In Llama (Lama Glama) Herds In Ayllu Pumasara, Saucari, Oruro, Magin Herrera Lopez

Theses and Dissertations

Camelid exploitation, despite its economic antiquity in our nation, recently has awakened greater interest in investigators. Currently, there are more than 2,500,000 llamas in the Altiplano, which is the highest number of camelids on the world. Production is organic, with the llama capital of Bolivia being the department of Oruro. This work's objective is to determine four production parameters: composition and structure of llama herds, percentage of births, cria [calf] mortality, and crias that reach 5 months old, in 3 communities of Ayllu Pumasa of Saucar’ province, department of Oruro. The llama-raising system is traditional and herds contain more than …

Effect Of Canihua (Chenopodium Pallidicaule Aellen), Wheat (Triticum Aestivum L.), And Soybean (Glycine Max M) Sprouts On The Feeding Of Guinea Pigs For Growth, Eddy Wilfredo Calle Ayma

Theses and Dissertations

This study occurred during July, August, and September in the Small Animals Investigation and Production Center (CIPAM), dependent of the Faculty of Agricultural, Livestock, and Veterinary Sciences of the Technical University of Oruro, of the Central Highland of Bolivia. In order to satisfy guinea pig nutrition requirements, which are 2800.00 kcal/kg of energy, 18.00% protein, 10.00% fiber, 1.00% calcium, and 0.60% phosphorus, the following ingredients were used in feed: 11.30% yellow corn, 30.00% milled wheat [Bromus catharticus], 14.00% soy cake, 32.00% wheat bran, 9.00% alfalfa flour, 1.80% bone flour, 1.00% conchilla, 0.50% common salt, and 0.40% methionine. The effect that …

Determination Of The Nutritional Level Of Calcium And Phosphorus In Llamas (Lama Glama) In Two Seasons In The Condoriri Agricultural Experiment Center, Gustavo Adolfo León Titichoca

Theses and Dissertations

The concentrations of calcium and phosphorus were determined in 12 llamas from the Condoriri Agricultural Experiment Center (Oruro Technical University) of Oruro, Bolivia during two seasons (wet and dry) and at three ages (newborn, juvenile, and adult). Samples of flesh were taken from the arm, leg, back, rib, and neck. Pasture samples (grassland and prairie) were used to determine the rate of biting of each animal studied. The blood samples were taken by extracting blood from a jugular vein. The minerals (Ca and P) in the flesh are highest in the wet season, 113.78 mg/100 g. The newborns were found …

Botanical And Chemical Composition Of Diets Selected By Alpacas (Lama Pacos) Pastured In The Ulla Ulla Wetlands, Lino Constancio López López

Theses and Dissertations

This study was done in the subtropical high Andes of Ulla Ulla. Its objectives were to determine the botanical composition, nutritional value, and preferred forages in the wetlands (hydromorphic and mesico) diet, by season (dry and wet), and by botanical family (12 families). Three-hundred sixty samples were collected from 6 alpacas with esophageal fistulations, and from them nutritional value and botanical composition were determined; the latter was compared with the botanical composition of the wetlands to determine preference. The results are highly significant (p<0.01) for botanical families, interaction between season and family, interaction of wetland type and family, and interaction of the season with wetland type and family. Species from the family Juncaceae were the most consumed with 27.78%, followed by moderate consumption of Cyperaceae (21.03%), Gramineae (15.02%), and Rosaceae (12.39%). Lower consumption occurred with Umbeliferaceae and Asteraceae with 6.61% and 5.64%, respectively, and the lowest consumption was of Plantaginaceae, Scrophulariaceae, minor plants, Gentianiaceae, unidentified plants, and Ranunculaceae with 2.86%, 2.50%, 2.17%, 2.08%, 1.03%, and 0.89%, respectively. The botanical families with the greatest preference value (PV) were Gramineae (PV=6.48), Scrophulariaceae (PV=8.75), Gentianiaceae (PV=7.55), and Ranunculaceae (PV=8.16). Proportional preference was observed in Juncaceae (PV=1.49), Cyperaceae (PV= -1.72), Rosaceae (PV=1.82), and Umbeliferaceae (PV= -0.82). The Asteraceae family was classified as a forced preference (PV= -6.29). The average nutritional value of the diet shows high values: 14.69% of raw protein, 14.58% ash, 85.42% organic matter, 21.43% raw fiber, and 31.00% neutral detergent fiber. It was concluded that alpacas efficiently select their diet for nutritional value, however, consumption is not appropriate, which results in negative live weight gain, especially in the dry season. On the other hand, the plants' preference values correspond to the lowest and one of the highest is a forced preference, according to the botanical composition of the wetland, which suggests that the preferred plants are repeatedly consumed in current continual pastures, and that alpacas are forced to consume unpalatable plants. This reflects on bad CANAPAS wetlands management in Ulla Ulla. Consequently, reversing this situation implies changes in pasture management and a reduction of the animal load.

Effect Of Bulbourethrectomy And Collection Frequency On Macro- And Microscopic Characteristics Of Llama (Lama Glama) Ejaculate, Víctor Efrain Gonzáles Vargas

Theses and Dissertations

This study occurred in the Rural Academic Unit-Tiahuanaco installations, of the Bolivian Catholic University-La Paz, Bolviia, with the objective of evaluating macro- and microscopic characteristics of sperm ejaculation from bulbourethrectomized llamas. Six q'ara-variety male llamas of 3, 4, and 5 years of age were used over 8 weeks during which they were fed with natural and cultivated pastures. Ejaculate was collected with an artificial vagina with stimulation (libido) of male llamas by female llamas, for macro- and microscopic evaluation (volume, pH, color, appearance, motility, concentration, and sperm vitality). The results obtained were: average volume of 0.55[+or-]0.36 ml, with a CV …

The Effects Of A One-Tesla Magnet On Human Fibroblast Cell Growth, Ashley Chaplin

Theses and Dissertations

The controversy regarding the ill effects of electromagnetic fields began in 1979 when Nancy Wertheimer and Ed Leeper claimed to have found a connection between childhood leukemia and power lines. In 1997, a group of researchers led by Martha Linet, M. D., attempted to provide evidence that there was no link between cancer and electromagnetic fields. The study showed that the risk of acute lymphoblastic leukemia did not escalate with increasing electromagnetic field levels in the children's homes. When compared to the Earth's static magnetic field of 0.5 Gauss, these fields were extremely small. Within the past decade, magnets have …

Evaluation Of Drip And Microasperation Irrigation Methods In Spinach (Spinaca Oleracea) And Swiss Lettuce (Valerianella Locusta) In Walipinis, Marina Roxana Luque Quispe

Theses and Dissertations

This study occurred at the Ventilla Ecologic farm, situated in the central Altiplano of the Murillo province, 26 km from La Paz. Its objectives were to evaluate two types of irrigation, by drip and by microasperation, in spinach and Swiss lettuce in Walipinis, and to conduct an economic analysis. The genetic material used was spinach (viroflay variety) and Swiss lettuce (large-leaved variety). Chapin irrigation belts and nebulizers were used, and two tensiometers were used to determine the soil moisture. Manual planting occurred on September 1, 2002, in all experimental areas. Seeds were dispersed in a continual flow with Swiss lettuce …

Evaluation Of Agronomic Performance Of Forage Grasses In Tiahuanaco, Justiniano Mamani Condori

Theses and Dissertations

Most families in the area of Tiahuanaco, Bolivia participate in livestock production, mainly cattle breeding. The primary feed sources for livestock in this area are native grasses, barley, and, to a smaller degree, oats. These grasses have low yield because of climate and soil conditions. This area does not have enough forage, especially during the dry season, and the animals are not regularly fed during the year. Consequently, animal production is low. This research was conducted during 2001 and 2002 at the Bolivian Catholic University campus, Farmer’s Academic Unit of Tiahuanaco, Ingavi Province, La Paz, Bolivia. Study site locations were …

Evaluation Of The Use Of Climatically Controlled Pankar-Huyus Modules For Cultivating Lettuce, Lucio Guillermo Rocabado Paco

Theses and Dissertations

Pankar-huyus are becoming an alternative solution to the Bolivian Altiplano's climate problems for agriculture and to help provide adequate nutrition for the inhabitants of this region. Pankar-huyus are small modules with mild ambient temperatures that cover roughly 4 m^2. They are built underground and are covered by agrofilm caps that, in this study, were partially opened by day and closed at night. This study evaluated the climactic parameters of minimum and maximum temperature, minimum and maximum relatively humidity inside the pankar-huyus, wind velocity outside and inside the pankar-huyus at 40 cm from the soil and at the level of the …

Regulation Of Fc Receptor Expression And Signaling On Murine Mast Cells, Hey Jin Chong

Theses and Dissertations

Mast cells have long been appreciated as the primary effector cells in allergy and asthma, and recently have been implicated in other inflammatory diseases. Using high density oligonucleotide probe arrays, we assessed genome-wide transcriptional profiles after FcεRI aggregation for 90 minutes, 5 hours and 24 hours. We describe novel gene regulation in response to FcεRI signaling, including altered expression of CD44, Pari, osteopontin, Nur77, E4BP4, and NDRG 1. In addition, the gene encoding FcεRI β was downregulated 5 hours after mast cell activation according to both microarray analysis and RPA, and western blotting confirmed the downregulation of the beta subunit …

Effects Of Emphysema And Chronic Hypoxemia On Skeletal Muscle Oxygen Supply And Demand, John D. Lowman Jr.

Theses and Dissertations

Skeletal muscle dysfunction in chronic obstructive pulmonary disease (COPD) is a condition in which peripheral skeletal muscle undergoes myopathic changes which impair muscle function, limit physical performance, and can lead to significant disability. While the etiology of the dysfunction is unknown, this study was conducted to test the hypothesis that chronic hypoxemia leads to alterations in oxygen transport and muscle function. A primary objective was to validate elastase-induced emphysema in rats as an animal model of skeletal muscle dysfunction in COPD.Arterial blood gases were used to determine the severity of hypoxemia and sodium dodecyl sulfate- polyacrylamide gel electrophoresis was used …

Surface Characteristics Of Bacillus Spores, Darlene Danette Sabio

Theses and Dissertations

Rapid isolation and identification of spores from various environmental samples is necessitated because anthrax spores can be used as biological weapons. The hydrophobic nature of spores may allow for their rapid concentration and partial purification from contaminating materials. In this study, spores from four taxonomic groups of Bacillaceae were isolated, purified and characterized for hydrophobicity by hexadecane partitioning, surface morphology by scanning electron microscopy, and steady-state fluorescence by spectroscopy. The morphology of spores was similar within taxonomic groups and dissimilar between groups. Spore hydrophobicity ranged from 0.3% to 65% and all spores had fluorescence emission peaks at 335 nm and …

An Accurate Vo2max Non-Exercise Regression Model For 18 To 65 Year Old Adults, Danielle I. Bradshaw

Theses and Dissertations

The purpose of this study was to develop a regression equation to predict VO2max based on non-exercise (N-EX) data. All participants (N = 100), aged 18-65 years old, successfully completed a maximal graded exercise test (GXT) to assess VO2max (mean ± SD; 39.96 mL∙kg-¹∙min&sup-1; ± 9.54 mL∙kg-¹∙min-¹). The N-EX data collected just before the maximal GXT included the participant's age, gender, body mass index (BMI), perceived functional ability (PFA) to walk, jog, or run given distances, and current physical activity (PA-R) level. Multiple linear regression generated the following N-EX prediction equation (R = .93, SEE = 3.45 mL∙kg-¹∙min-¹, %SEE = …

Analysis Of Weight-Related Advertisements And Nutrition Articles In Popular Women's Magazines, Jennifer Cardall Jacobson

Theses and Dissertations

Objective: The purpose of this study was to discover whether the weight-related messages and themes from food advertisements in women's magazines reinforce or contradict the messages communicated in nutrition-related articles focusing on weight control within the same magazines.

Design: Content analysis of 48 issues of weight-related content six popular women's magazines, 2001-2002. There were three magazines in each of two categories, "homemaking" and "health." All food and nutrition advertisements were analyzed (n=954), along with all nutrition articles (n=336). Advertisements and articles were identified as being either weight-related or non-weight-related. Type of food/product advertised, claims used to market the product, and …

Epidemiology Of Ustilago Bullata Berk. On Bromus Tectorum L. And Implication For Biological Control, Toupta Boguena

Theses and Dissertations

The seedling-infecting pathogen Ustilago bullata Berk. is a naturally occurring biological control agent for cheatgrass (Bromus tectorum L.). The effects of temperature and nutrients on pathogen teliospore germination behavior and the effects of temperature on host seed germination were examined. The effects of temperature on sporidial proliferation, host infection in a temperature-controlled environment and in a field setting for eight populations were investigated. The infection success of Ustilago bullata on Bromus tectorum in cultivated fields as a function of seeding date, inoculation method, inoculum density, supplemental watering, and litter was also investigated. Teliospores germinated faster on potato dextrose agar than …

Physical Activity And Changes In Abdominal Fat Over 18 Months: A Prospective Study Of Middle-Aged Women, Lance Eric Davidson

Theses and Dissertations

Objective: To investigate the extent to which changes in physical activity predict changes in abdominal fat in women over an 18-month period, while statistically controlling the effects of possible confounders, such as age, total body fat percent, and energy intake. Design: A prospective cohort design over 18 months. There was no intervention or treatment. Changes in objectively-measured physical activity were used to predict changes in abdominal fat over the study period. Subjects: 110 healthy, middle-aged women (mean: 41.3±3.3 yrs), primarily Caucasian, educated, and married. Measurements: An objective measure of physical activity (ACT) using CSA accelerometers, worn continuously for 7 consecutive …

An Analysis Of Microbial Contamination In Military Aviation Fuel Systems, Harold W. Graef

Theses and Dissertations

Military aviation fuel systems can be an ideal environment for microorganisms. Microbial growth in hydrocarbon fuel systems arises because of the impracticality of keeping fuel tanks sterile and the inevitable presence of water from condensation. Microbial contaminants in aviation fuel systems are a concern because of their potential to degrade the fuel, accelerate tank corrosion, and threaten flight safety. This research addresses the concern of using more environmentally friendly Fuel System Icing Inhibitors (FSII), which are also biocidal. Are significant levels of microorganisms growing in military aviation fuel systems, and if so, are there any common variables? Forty aviation fuel …

Stochastic Intra-Cellular Modeling, Thomas E. Hopkins

Theses and Dissertations

Air Force personnel may sometimes come into contact with potentially harmful chemicals while performing their duties. Of course the Air Force desires to keep any potential health risks to its members to a minimum. To this end the Air Force would like to identify which chemicals are toxic, their level of toxicity, and the processes by which these chemicals disrupt normal biological activities at the cellular level. The development of mathematical models can be of great benefit to toxicity studies. Because real world systems involve randomness, that is noise, and the desire is to create mathematical models to represent those …

Deterministic Intracellular Modeling, Jacqueline B. Young

Theses and Dissertations

The United States Air Force is interested in the potential side effects at the cellular level from exposure to mission-essential chemicals. Presently, Air Force toxicology studies are conducted to help shed light in identifying potential hazards to workers. However, it takes a considerable amount of money, resources, and time to obtain and analyze experimental results from toxicology studies. The necessity for innovative methods that enable researchers to more effectively generate and analyze data is apparent.

A System Dynamics Investigation Of Genetic Drift And Translocation In The Red-Cockaded Woodpecker Metapopulation, Jason E. Yates

Theses and Dissertations

The red-cockaded woodpecker (Picoides borealis) is classified under the Endangered Species Act of 1973 as an endangered species As such, the red-cockaded woodpecker (RCW) is afforded strict protection in an effort to return the RCW population to a self-sustainable level. Endemic to southeastern United States mature pine forests, the presence of the RCW impacts the operations of many Department of Defense (DoD) installations. A particular challenge in sustaining what are often small populations of RCW at these locations is the loss of genetic variation due to genetic drift. The optimal method for mitigating such loss is through the artificial immigration, …

Implementation And Validation Of A Real-Time Wireless Non-Invasive Physiological Monitoring In A High-G Environment, H. Aydin Akcivi

Theses and Dissertations

The overall purpose of this research is to develop a system capable of real-time personal positioning and physiological monitoring. The system will be composed of a shirt having non-invasive physiological sensors, Global Positioning System (GPS) receiver, wireless data transceiver, and real-time PC-based control station. The specific purpose of this research phase was to determine the performance capabilities of a modified LifeShirt™ alone (without GPS) in a high gravitational force environment with the data being sent wirelessly in real-time. The LifeShirt™ was modified with a real time wireless transmission system.

Influence Of Three Soil Types On The Physical, Chemical, And Organoleptic Characteristics Of Two Varieties Of Kidney Tomato Hydroponically Cultivated Under A Panqar Huyu System, Carlos Fernando Yepez Vallejo

Theses and Dissertations

One of the largest problems that face communities located high in the Andres Mountain range is the lack of crop diversity due to the climate characteristics of this zone. Because of this, the Benson Institute created a small subterranean greenhouse called a "panqar-huyu," which in Aymara means "flower garden," that allows cultivation of small and medium-sized crops. This study's objective is to modify and adapt this system to cultivate table (kidney) tomatoes, for which one was enlarged, a drip irrigation system was installed, channels were dug to drain groundwater from the interior, etc. This study was done in the community …

The Effect Of Aposematic Coloration On The Food Preference Of Aphelocoma Coerulescens, The Florida Scrub Jay, Mary Elizabeth Hill

Theses and Dissertations

Aposematic coloration, commonly observed in noxious organisms, serves as a warning to predators to avoid attacking specific prey. This coloration is conspicuous in nature, with one of the most common examples being a red and black pattern. The plant Abrus precatorius exhibits this warning signal in its seeds, which resemble lady beetles and contain the concentrated toxin abrin. Aphelocoma coerulescens, an endemic and federally threatened bird in the state of Florida, shares a similar distribution with Abrus precatorius, making interaction between the two species possible. Using an edible model of the Abrus seed, this potential interaction was tested in Martin …

Costs Of Feeding Guinea Pigs In Two Meat-Producing Populations In The Field And Experimental Station, Sara Taborga Galindo

Theses and Dissertations

The cost of feeding has been pointed out as the most trascendet investment inside a guinea-pig production. The present work carries out with the purpose to determine and to quantify feeding costs, total costs of production and profitability of Tamborada and MEJOCUY guinea-pig populations. In the stage of suckling period, breed and gestation, experimental station and field. The result of feeding cost was 0.05 and 0.03 $US in suckling period (14 days) for Tamborada and MEJOCUY population, in breeding (42 days) it was 0.14 $US in both populations and in gestation (67 days), it was 0.42 and 0.40 $US. In …