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Articles 4201 - 4230 of 5010

Full-Text Articles in Life Sciences

Soil Stable Carbon Isoptope Analysis Of Landscape Features At Aguateca, Guatemala, David R. Wright Mar 2006

Soil Stable Carbon Isoptope Analysis Of Landscape Features At Aguateca, Guatemala, David R. Wright

Theses and Dissertations

The ancient Maya of the Classic period (1700-1050 B.P.) relied on maize agriculture to support their populations. The agricultural systems they employed to produce that maize varied in form and degree of intensity, with more productive forms of agriculture needed in the Late Classic period (1350-1050 B.P.) to sustain the peaking population. It is likely that the ancient systems of production agriculture contributed to environmental degradation that in turn contributed to the pressures that culminated in the collapse of the civilization. In this study, stable carbon isotope ratios contained in the soil organic matter were used to further investigate ancient …

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The Role Of Pax3 In Neuronal Differentiation Of The Ophthalmic (Opv) Trigeminal Placode And Neural Tube During Chicken Embryonic Development, James R. Bradshaw Mar 2006

The Role Of Pax3 In Neuronal Differentiation Of The Ophthalmic (Opv) Trigeminal Placode And Neural Tube During Chicken Embryonic Development, James R. Bradshaw

Theses and Dissertations

Pax3 has been used as a valuable marker in research aimed at understanding tissue interactions involved in trigeminal ophthalmic (opV) placode development. While Pax3 expression coincides with opV neuron specification, the function of Pax3 in these cells has not previously been investigated. Splotch mutant mice (which lack Pax3) have a reduced trigeminal ganglion; however it is not clear whether this reduction is due to neural crest or placode cells. We have used electroporation in the chick model system to block or ectopically express Pax3 at key times in opV placode development. Using several markers of placode cell differentiation, we have …

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Membrane-Based Protein Preconcentration Microfluidic Devices, Yi Li Mar 2006

Membrane-Based Protein Preconcentration Microfluidic Devices, Yi Li

Theses and Dissertations

Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced …

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The Dogma Of The 30 Meter Riparian Buffer: The Case Of The Boreal Toad (Bufo Boreas Boreas), Michael C. Goates Mar 2006

The Dogma Of The 30 Meter Riparian Buffer: The Case Of The Boreal Toad (Bufo Boreas Boreas), Michael C. Goates

Theses and Dissertations

We tested the adequacy of standard 30 m riparian buffers for semi-aquatic vertebrate species, using the boreal toad (Bufo boreas boreas) as an example. We monitored toad populations in south-central Utah using radio telemetry during the summers of 2003 and 2004. We found 30 m buffers inadequate for protecting boreal toads and suggest this is likely true for other species as well. Managers must consider several factors when constructing buffers: (1) Buffer requirements may vary by time of year. While we located toads most often in wet habitats, toads commonly utilized upland habitats in late summer, occasionally at distances greater …

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An Exploration Of The Effects Of Genetic Drift On The Endangered Red-Cockaded Woodpecker, Ryan E. Nelson Mar 2006

An Exploration Of The Effects Of Genetic Drift On The Endangered Red-Cockaded Woodpecker, Ryan E. Nelson

Theses and Dissertations

The Red-cockaded Woodpecker (RCW) (Picoides borealis) is classified as an endangered species under the Endangered Species Act of 1973 due to habitat fragmentation and prescribed burn suppression. Given the endangerment of the RCW, the genetic variation of the small populations will decrease over time due to random genetic drift. The only method of battling the negative effects of random genetic drift is to move, or translocate, genetically diverse birds into the fragmented populations thereby increasing the genetic diversity of the small populations. The objective of this research effort was to explore the effects of random genetic drift on small RCW …

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Comparative Analysis Of Airborne Chemical Exposure To Air Force Small Arms Range Instructors, Eric J. Cameron Mar 2006

Comparative Analysis Of Airborne Chemical Exposure To Air Force Small Arms Range Instructors, Eric J. Cameron

Theses and Dissertations

This study examines airborne chemical exposure to Air Force small arms range instructors during M16 firing of lead and lead-free bullets. Historical range information collected from 63 active duty Air Force bases identified that two thirds of the Air Force military ranges within the Continental United States are currently firing lead-free ammunition. Average 8-hr TWA exposures for lead during M16 firing of leaded ammunition were 17% of the Occupational Exposure Limit (OEL). Task exposures, representing worst case conditions, were found to be 1.2 time the OEL. Two indoor and four outdoor firing ranges currently firing frangible lead-free ammunition were evaluated …

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Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr. Mar 2006

Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr.

Theses and Dissertations

Chlorinated hydrocarbons and their degradation products are among of the most common organic groundwater contaminates in the United States. These compounds attack the central nervous system in animals and can affect the photosynthesis of plants. These compounds are also resistant to degradation in the environment and, because of this, pose a risk to any ecosystem in which they are present. This study identified the dominant microbial species in a constructed treatment wetland at Wright-Patterson AFB, Dayton, Ohio using 16S rRNA gene sequence analysis. Samples were taken from three different depths and during each of the four seasons. These samples were …

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In Vitro Toxicity Of Aluminum Nanoparticles In Rat Alveolar Macrophages, Andrew J. Wagner Mar 2006

In Vitro Toxicity Of Aluminum Nanoparticles In Rat Alveolar Macrophages, Andrew J. Wagner

Theses and Dissertations

Nanomaterials, which are by definition in the 1 - 100 nanometer range, have numerous possible benefits to society, but currently there is a lack of data that characterizes these materials effects on human health and environment. In general nanomaterials are of interest to the Air Force because of their applications in electronics, sensors, munitions and energetic/reactive systems. Nanoparticles such as aluminum have been considered for enhancing propulsion in solid rocket fuel. To date, only a few studies have looked at the toxicological effects of direct exposure to nanoparticles, none with aluminum. It is important to increase the understanding of the …

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Measurement, Characterization, And Source Apportionment Of The Major Chemical Components Of Fine Particulate Material, Including Semi-Volatile Species, Brett D. Grover Feb 2006

Measurement, Characterization, And Source Apportionment Of The Major Chemical Components Of Fine Particulate Material, Including Semi-Volatile Species, Brett D. Grover

Theses and Dissertations

The promulgation of revised standards for atmospheric fine particles (PM2.5) by the US EPA has sparked renewed interest in the ability to accurately measure and characterize suspended atmospheric particulate matter. Semi-volatile material (SVM), consisting of ammonium nitrate and semi-volatile organic material (SVOM), is not accurately measured by EPA accepted methods such as the Federal reference method (FRM) or Tapered Element Oscillating Microbalance (TEOM). However, SVM is often a major fraction of urban aerosols. Recent advances in atmospheric sampling instrumentation allowed for the semi-continuous characterization of urban PM2.5, including SVM. The Filter Dynamic Measurement System (FDMS) was shown to measure total …

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Characterization, Functionalization And Applications Of Alkyl Monolayers On Silicon Surfaces, Guilin Jiang Feb 2006

Characterization, Functionalization And Applications Of Alkyl Monolayers On Silicon Surfaces, Guilin Jiang

Theses and Dissertations

Investigations were performed on the stability, mechanism of formation and an application of alkyl monolayers chemomechanically prepared on silicon surfaces. A new method of surface modification, laser-activation modification of surfaces (LAMS), and multivariate analyses of time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of LAMS spots were also reported. X-ray photoelectron spectroscopy (XPS) and other data show that alkyl monolayers prepared by scribing silicon under 1-iodoalkanes and 1-alkenes were stable over extended periods of time to air, water, a boiling acid and Al Ka X-rays. The stability is attributed to direct Si-C bonding in the monolayers. The observation that the oxygen …

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Characterization Of The Role Of Sox9 In Cartilage-Specific Gene Regulation, Mary Ann Genzer Jan 2006

Characterization Of The Role Of Sox9 In Cartilage-Specific Gene Regulation, Mary Ann Genzer

Theses and Dissertations

Although advances have been made toward understanding the complex mechanisms that regulate the process of DNA transcription, the specific mechanisms of activation for many individual genes remain unknown. In this study, we focus on the role the transcription factor SOX9 plays in activating cartilage-specific genes, specifically Col9a1 and Cartilage Link Protein (CRTL1). Previously, enhancers of these genes containing single SOX9 binding sites were shown to be activated through SOX9 binding. However, the hypothesis was made that in cartilage-specific genes dimeric SOX9, as opposed to monomeric SOX9, is necessary for activation. We identified a putative binding site adjacent to each of …

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Apparent Digestibility In Lamas (Lama Glama), Fed With Brazilian Grass And Wheat In The C.E.A.C., William Edson Velez Contacayo Jan 2006

Apparent Digestibility In Lamas (Lama Glama), Fed With Brazilian Grass And Wheat In The C.E.A.C., William Edson Velez Contacayo

Theses and Dissertations

Considering the activity of introducing genetic resources with a high potential for yield, and with a wide adaptation range, it is possible to strengthen the cattle production system, through the production of adequate forage in quality, and quantity. Brazilian grass was introduced to Bolivia in 1974 for demonstrating excellent forage characteristics, and great adaptation qualities to the different ecosystems of the Andes. The material was introduced through the Experimental Station of Patacamaya, with their material supplied through the Experimental Station of Obonuco (Colombia). The following study was conducted at the Agropecuary Experimental Center of Condori, located 49 km. north of …

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Effect Of Early Weaning On Piglet Weight Gain And Fertility In Sows (Sus Scrofa) In Carmen Pampa Of Nor Yungas - La Paz, Darwin Juan Luna Isita Jan 2006

Effect Of Early Weaning On Piglet Weight Gain And Fertility In Sows (Sus Scrofa) In Carmen Pampa Of Nor Yungas - La Paz, Darwin Juan Luna Isita

Theses and Dissertations

This study was carried out in swine production facilities of the School of Veterinary Medicine and Zootechnic Studies at the Rural Academic Unit of Carmen Pampa. The objective of this study was to evaluate the effects of weaning male and female piglets at different times on their weight gain and the fertility of the sows. Four three year old sows that had a total of 40 piglets (25 males and 15 females) were used. The piglets were distributed across the following treatments: T1 - weaning 9 piglets (5 males and 4 females) at 56 days, T2 - weaning 13 piglets …

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Agronomic Evaluation And Comparison Of The Yield Of Six Multiannual Forage Species, Under Dry Land Conditions In The Ingavi Province, Freddy Óscar Paye Paye Jan 2006

Agronomic Evaluation And Comparison Of The Yield Of Six Multiannual Forage Species, Under Dry Land Conditions In The Ingavi Province, Freddy Óscar Paye Paye

Theses and Dissertations

The agronomical evaluation and the comparative work of six multiannual forage species was conducted in the facilities of the Benson Institute, in the community of Contorno de Letanias, Ingavi province, La Paz. The objectives were to evaluate the agronomic behavior, as well as to evaluate the dry matter (DM) yield of six multiannual forage species (grasses) under local conditions, and to determine the partial cost of production of the studied species. The annual mean temperature was 9.14°C, and 517.6 mm of precipitation. The test was conducted under the random block design test, with six different forage species, and three repetitions. …

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Evaluation Of The Seed Production Of Tall Grasses Tolerant To Drought And Salinity In The Tamborada Region - Cochabamba, Magali Vallejos Arnez Jan 2006

Evaluation Of The Seed Production Of Tall Grasses Tolerant To Drought And Salinity In The Tamborada Region - Cochabamba, Magali Vallejos Arnez

Theses and Dissertations

This study was conducted on plots established by the Benson Institute in the Tamborada region of Cochabamba, Bolivia. The objective of the study was to evaluate the seed production of tall grasses that are tolerant to drought and salinity. A design of randomized blocks in divided plots with and without fertilizer was used. The results showed that the species with the greatest number of stems and inflorescence was Bromus inermis with 10.9 and 88.67 respectively. It was determined that fertilization has an effect on plant height. Furthermore, the species with the greatest seed yield was Festuca arundinacea with 30.4 kg/ha.

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Agronomic Growth Characteristics Of Two Varieties Of Baby Carrots (Daucus Carota L.) At Three Planting Densities In Walipini (Ingavi Province), María Eugenia Valdez Cuba Jan 2006

Agronomic Growth Characteristics Of Two Varieties Of Baby Carrots (Daucus Carota L.) At Three Planting Densities In Walipini (Ingavi Province), María Eugenia Valdez Cuba

Theses and Dissertations

Our objective is to study the agronomic growth characteristics of two varieties of baby carrots at three planting densities. The study site is in the area of Walipini, town of Lentanias, province of Ingavi, department of La Paz, Bolivia. In this investigation, the two varieties of baby carrots used were ‘Amsterdam Forcing 3-Minicor’ and ‘Thumbelina’ (Daucus carota var. sativus). Both varieties were planted at densities of 333 plants/m2, 166 plants/m2 and 111 plants/m2 with a planting interval of two days. Statistical differences were observed for the following variables: plant height, root length, root diameter, base diameter, leaf yield, and root …

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Role Of Mdm2 In Cell Growth Regulation, Rebecca Anne Frum Jan 2006

Role Of Mdm2 In Cell Growth Regulation, Rebecca Anne Frum

Theses and Dissertations

MDM2 has been shown to induce G0-Gl/S phase arrest. To determine the cell cycle step targeted by MDM2, flow cytometry was employed to detect induction of events during the G1-S phase transition in MDM2-arrested cells. MDM2 overexpression does not prevent expression of cyclin D, cyclin D-CDK mediated phosphorylation of Rb or cyclin E in normal, immortal or tumor-derived cells. However, MDM2 down-regulates cyclin A expression specifically in normal cells, which is associated with G1 arrest. The domain of MDM2 capable of this function is located within its N-terminal 58-109 amino acids. To down-regulate cyclin A, MDM2 requires a functional pl6/Brg …

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Novel Cancer Therapeutics, The Generation Of Ros, And Cell Survival, Clint Mitchell Jan 2006

Novel Cancer Therapeutics, The Generation Of Ros, And Cell Survival, Clint Mitchell

Theses and Dissertations

The impact of Ad.mda-7 on the survival of renal cell carcinoma lines (RCC), primary renal epithelial cells, glioblastoma multiforme lines (GBM), and primary rodent astrocytes is unknown. The present studies examine whether the GST fusion protein, GST-MDA-7, and the adenovirus, Ad.mda-7, altered the growth and survival of the A498 and UOK121N RCC lines or radiosensitized GBM, respectively. Due to previous findings that the RCC lines, but not primary renal epithelial cells, were resistant to type 5 adenoviral infection, we used purified GST-MDA-7 protein to show that GST-MDA-7, but not GST, caused a dose-dependent reduction in A498 and UOK121N proliferation but …

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Degenerate Oligonucleotide Primed - Polymerase Chain Reaction Evaluation And Optimization To Improve Downstream Forensic Str Analysis Of Low Quality/Low Quantity Dna, Lindsay Paige Thompson Jan 2006

Degenerate Oligonucleotide Primed - Polymerase Chain Reaction Evaluation And Optimization To Improve Downstream Forensic Str Analysis Of Low Quality/Low Quantity Dna, Lindsay Paige Thompson

Theses and Dissertations

When forensic biological samples yield low quality/low quantity DNA, thecurrent STR analysis methods do not generate acceptable profiles. Whole genomeamplification can be used to pre-amplify the entire genome for downstream analyses. A commercially available kit for DOP-PCR, a form of WGA, is currently being used in the clinical for downstream single locus targets. Forensic analyses utilize a multiplex amplification. This study determined that the "home brew" created by our lab performs the same as the commercially available kit. Future optimization studies of DOP-PCR can utilize this "home brew". Additionally, this research determined that a 10 second increase in electrokinetic injection …

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Acquisition And Retention Of Bacterial Spores (Bacillus Atrophaeus) By Eight Insect Species, Kieron Marie Torres Jan 2006

Acquisition And Retention Of Bacterial Spores (Bacillus Atrophaeus) By Eight Insect Species, Kieron Marie Torres

Theses and Dissertations

Acquisition and retention of spores of an anthrax surrogate, Bacillus atrophaeus Nakamura ("BG") were evaluated in eight insect species. Species included: house cricket (Acheta domesticus L.), German cockroach (Blatella germanica L.), common house fly (Musca domestics L.), blue bottle fly (Calliphora vomitoria L.), hairy rove beetle Creophilus maxillosus L.), yellow mealworm (Tenebrio molitor L.), common paper wasps (Polistes exclamans exclamans Viereck), red paper wasps (Polistes Carolina L.), red harvester ant (Pogonymyrmex barbatus Smith). Individual insects were offered BG-treated food and sacrificed at specified time intervals following one, two or three meals. Resulting samples were surface-washed five consecutive times then homogenized …

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Genetic Assessment Of Rare Blackbanded Sunfish (Enneacanthus Chaetodon) Populations In Virginia, Diana Marie Kercher Jan 2006

Genetic Assessment Of Rare Blackbanded Sunfish (Enneacanthus Chaetodon) Populations In Virginia, Diana Marie Kercher

Theses and Dissertations

Enneacanthus chaetodon, the blackbanded sunfish, has become increasingly rare throughout its distribution in the Eastern United States. In Virginia, E. chaetodon maintains an endangered status and individuals persist in six populations. Mitochondrial DNA (mtDNA) and microsatellite data were assessed to determine the genetic characters and gene diversity of the Virginia populations. The results of these analyses were then compared to five additional populations; four from New Jersey and one from North Carolina that were known to have relatively good fitness and were not impacted severely by habitat alteration. The results of this study are relevant to selection of proper management …

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Invasion Genetics Of The Blue Catfish (Ictalurus Furcatus) Range Expansion Into Large River Ecosystems Of The Chesapeake Bay Watershed, Colleen Beth Higgins Jan 2006

Invasion Genetics Of The Blue Catfish (Ictalurus Furcatus) Range Expansion Into Large River Ecosystems Of The Chesapeake Bay Watershed, Colleen Beth Higgins

Theses and Dissertations

The blue catfish, Ictalurus furcatus (Ictaluridae), is ranked among the most invasive, nonnative species of concern in the Chesapeake Bay watershed. This species, intentionally introduced to three major tributaries and a number of impoundments between 1974 and 1989 for sport fishing, has spread into three additional tributaries. Using samples from the introduced tributary populations as a baseline, we evaluated microsatellite genetic variation in light of demographic and ecological data to elucidate the potential sources of the invasive I. furcatus populations. In general, the populations surveyed in the Chesapeake Bay watershed were considerably more inbred (F ranged from 0.03 - 0.27) …

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Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier Jan 2006

Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier

Theses and Dissertations

Degenerate Oligonucleotide Primed-PCR (DOP-PCR) can potentially enhance analysis of low copy number DNA samples. Theoretically, this procedure replicates fragments of the genome that can then be used for downstream multiplex STR analysis. The objective of this study is to optimize DOP-PCR by examining ramplelongation times and cycle numbers in the non-specific amplification portion of DOP-PCR, and by modifying the degenerate primer. Additionally, other methods such as Multiple Displacement Amplification (MDA) and Low Copy Number PCR (LCN PCR) were examined for their ability to create accurate DNA profiles from low DNA input amounts. Increasing the ramplelongation times showed no effect on …

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Application Of Different Levels Of Byproducts From Quinua (Chenopodium Quinoa Willd.) In The Preparation Of Rations For Guinea Pigs (Cavia Porcellus L.) In The Growing And Fattening Stages, Grover Antonio Aduviri Paredes Jan 2006

Application Of Different Levels Of Byproducts From Quinua (Chenopodium Quinoa Willd.) In The Preparation Of Rations For Guinea Pigs (Cavia Porcellus L.) In The Growing And Fattening Stages, Grover Antonio Aduviri Paredes

Theses and Dissertations

From quinua crop residue it is possible to obtain byproducts that can be used to feed small animals, especially guinea pigs. These byproducts can become an alternative feed to ameliorate food deficiencies generally present in the Altiplano. This study compared the effect of consumed quinua meal rations on guinea pig body weight increase and food and economic conversion. This project was developed in two phases: pre-experimental and experimental. In the first phase, genetic material appropriate for the project and the initial rations for consumption was acquired. The second phase included the experimental design where the treatments were developed. The study …

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Apparent Digestibility In Vicunas Fed With Soft Straw And Alfalfa In The C.E.A.C., Felisa Capriles Apaza Jan 2006

Apparent Digestibility In Vicunas Fed With Soft Straw And Alfalfa In The C.E.A.C., Felisa Capriles Apaza

Theses and Dissertations

South American camelids are a source of fiber and meat for the inhabitants of the Andean region of Bolivia, Argentina, and Chile. When camelids are well fed they efficiently convert the Andean flora into meat with high protein content. Vicuñas have a great capacity to produce wool to be used as a high quality fiber. However, the yield of vicuña meat production is generally low. There have been several studies concerning nutritional forage sources for camelids, resulting in improved nutrition, decreased morbidity and mortality, and intensified production systems. Soft straw is a source of tough forage with low protein content. …

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Functional Consequences Of Matrix Metalloproteinase-1 Over-Expression In Human Gliomas, Emily Mullet Jan 2006

Functional Consequences Of Matrix Metalloproteinase-1 Over-Expression In Human Gliomas, Emily Mullet

Theses and Dissertations

Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western …

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Metabolic Profile And Nitrogen Balance In Llamas (Lama Glama) Fed With Jipi Quinua And Barley Hay, Viacha Municipality - Department Of La Paz, Meliton Ajata Avircata Jan 2006

Metabolic Profile And Nitrogen Balance In Llamas (Lama Glama) Fed With Jipi Quinua And Barley Hay, Viacha Municipality - Department Of La Paz, Meliton Ajata Avircata

Theses and Dissertations

This research was completed in Letanías, Viacha, La Paz, Bolivia, to determine the metabolic profile in blood plasma (total protein, glucose, urea, albumin, total lipids, creatinine, triglycerides, and cholesterol concentrations), live weight gain, and Nitrogen balance in adult llamas fed with 4 rations of barley and quinoa residue. Eight adult (4-5 year old) Q’ara type llamas were used in this study. Study llamas were trained to stay in metabolic cages with feces collection harnesses. Llamas were given one of the following feed rations: ration A (20% quinoa residue, 80% barley), ration B (40% quinoa residue, 60% barley), ration C (60% …

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Effect Of The Use Of Amaranth Leaf Flour In The Development Of A Diet For Guinea Pigs, Verónica Del Rocío Guerrero Carrera Jan 2006

Effect Of The Use Of Amaranth Leaf Flour In The Development Of A Diet For Guinea Pigs, Verónica Del Rocío Guerrero Carrera

Theses and Dissertations

This study was carried out in the San Clemente community. The objective was to analyze the effect of using amaranth leaf flour in the creation of a diet for guinea pigs. The field work was done over a period of 12 months and was done in two phases: Development of the diet: this was done by growing the amaranth, cutting it, and dehydrating it until flour was obtained. Then, it was mixed into the diet in accordance with the pre-determined formula along with the other respective materials. Handling of guinea pigs: This was done in the shed of the San …

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Effect Of Chemical Fertilization And Organic Amaranth (Amaranthus Caudatus L.) Var. Alegria Iniap Chaltura, Andrade Andrade Pablo Raúl Jan 2006

Effect Of Chemical Fertilization And Organic Amaranth (Amaranthus Caudatus L.) Var. Alegria Iniap Chaltura, Andrade Andrade Pablo Raúl

Theses and Dissertations

The present investigation was made in the Experimental Farm "La Pradera", located in the province of Imbabura, the parish of San José de Chaltura, to 1350 meters above sea level and an average temperature of 16, 4⁰ C. To analyze the effect of the chemical and organic fertilization in amaranth of variety INIAP "Alegría" was the primary target of the investigation. The field work was made in a lapse of 6 months. The design of complete blocks (DBCA) with 15 treatments was used at random and three repetitions by treatment. The experimental unit was 18 m². It was evaluated by …

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Modulation Of The Serotonin Reuptake Transporter In Raw264.7 Macrophages, Sienna Marie Arenas Malubay Jan 2006

Modulation Of The Serotonin Reuptake Transporter In Raw264.7 Macrophages, Sienna Marie Arenas Malubay

Theses and Dissertations

Serotonin (5-HT) plays an important role as both a neurotransmitter and animmune modulator. The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the …

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