Life Sciences Commons^™

Aiding Semantic Memory Creation With Navigational Context, Thomas Benjamin Lyle Wasden

Theses and Dissertations

While we have traditionally understood the hippocampus to be involved in memory and navigation, it also appears that it has a role in language processing, creation and prediction. An obvious explanation for this is that language is impossible if linguistic signs cannot be remembered and retrieved. Because linguistic signs are definitionally biologically neutral or arbitrary, we must use the brain's apparatus for learning and storing information from the external world to store and retrieve them. Although plausible, this explanation fails to take into account the hippocampus' role in navigation as a contributing element in the processing, storage and retrieval of …

Families In The Sky: Investigating The Population Structure Of Pinus Longaeva, Samuel Arnold Decker

Theses and Dissertations

In the Western United States, the Great Basin is a geographic feature that is home to a variety of unique species, including Great Basin bristlecone pine (Pinus longaeva, PILO). P. longaeva is well known for the age of some of the trees, some of which are over 4000 years old. In this study 17 microsatellite markers (simple sequence repeat, SSR) are identified and used to genotype a selection of 480 individuals spread across 24 PILO populations and 60 individuals divided equally between 2 outgroup species, Pinus aristata and Pinus flexilis. One population of PILO, HMW, shows more genetic similarity with …

Comparative Sequence Analysis Elucidates The Evolutionary Patterns Of Yersinia Pestis In New Mexico Over Thirty-Two Years, M. Elizabeth Warren

Theses and Dissertations

Yersinia pestis, a gram-negative bacterium, is the causative agent of plague. Y. pestis is a zoonotic pathogen that occasionally infects humans, and is endemic in the western United States. History gives evidence of three main plague pandemics. The first, originating in Egypt in 541AD, is known as the Justinian plague. The second, perhaps most well-known, is thought to have emerged in 1347AD in China, and is called the Black Death. The third, and current plague pandemic, also emerged in China in 1855. In 1899, Y. pestis was established in California, and the plague in other parts of America evolved from …

The Influence Of Thc, Opioids, And Age On The Plasticity Of Excitatory Inputs To Ventral Tegmental Area Gaba Neurons, Isaac Brent Ostlund

Theses and Dissertations

Adults exhibit significant differences in drug vulnerability, learning ability, and emotive processing than adolescents. Long-lasting synaptic changes, including long-term depression (LTD), can endure many hours and are believed to be key to encoding memories and persistent cognitive changes such as addiction. The ventral tegmental area (VTA) is the primary source of midbrain dopamine (DA) and is regulated by local inhibitory GABA neurons. GABA regulation can decrease, resulting in dis-inhibition of DA neurons and increased feelings of reward, learning, or salience attachment to memories. Endocannabinoids (eCBs) are signaling molecules that often result in synaptic plasticity changes, and the eCB system has …

Local Adaptation Of Blue Penstemon: Molecular And Morphological Characterizations Of A Potential Restoration Species For The Northern Basin And Range And Snake River Plain, Jason Mark Stettler

Theses and Dissertations

Penstemon is one of North America's largest endemic genera with over 280 described species. These species are distributed throughout most of North America from the Arctic northern latitudes to tropics of Central America. The genus has historically been divided into six subgenera, but has recently been reorganized into two subgenera following some recent phylogenetic studies. I made a comprehensive assessment of the Penstemon genus' geographic distribution utilizing herbaria databases by ecoregion to discuss the general ecologic adaptations of each historic subgenera. I also assessed the Penstemon genus' bee pollinator diversity utilizing online databases of bee specimen collections associated with Penstemon …

Functional Facets Of Intergenic Hairpin Structures In Genus Caulobacter, Geetha Saarunya Clarke

Theses and Dissertations

DNA replication, recombination and repairs maintain bacterial genome stability. But these processes may also induce genome rearrangements leading to inter andintra chromosomal structural variations. Genus Caulobacter undergoes extensive genome rearrangements. Genomic studies in bacteria usually focus on the codingregions, but there is important information present in the intergenic DNA spaces inaddition to the regulatory elements involved in transcription. Recently, Ely published a new model for recombination in genus Caulobacter with simultaneousloss and gain of genes resulting from preferential recombination at non- homologous regions flanked by regions of homology. In my dissertation, I observedand catalogued hairpin structures at known sites of …

Defining Molecular And Morphological Phenotypes In Ash1l-Deficient Human Neurons, Foster D. Ritchie

Theses and Dissertations

Autism Spectrum Disorders (ASD) are highly heritable neurodevelopmental disorders characterized by social and verbal impairments as well as repetitive behaviors. Large human genetic studies showed that genes encoding chromatin and transcriptional regulatory proteins are among the most commonly mutated genes in ASD. Absent, Small, or Homeotic 1-like (ASH1L) is a histone methyltransferase that has previously been identified as a major genetic risk factor for ASD. ASH1L di-methylates Histone H3 on Lysine 36 (H3K36me2) and tri-methylates Histone H3 on Lysine 4 (H3K4me3), which mainly coincides with transcriptional activation. ASH1L is a Trithorax protein that is proposed to counteract the activity of …

Cryptic Cryptophytes: The Plasticity In How Cryptophytes Respond To Changes In Resource Availability, Rachel Ann-Marie Schomaker

Theses and Dissertations

Resource variability and availability often drives competition within ecosystems, which can lead to the diversification of organismal niches and physiological capabilities. In aquatic systems, common resources that photosynthetic organisms, such as algae, compete for are light and carbon. Both the spectral characteristics (color) and carbon concentrations of an aquatic system vary with time and space, which means that algae need to be able to respond to changes in resource availability to survive. Using a group of ubiquitous unicellular eukaryotes known as cryptophytes, we investigated both how cryptophytes respond to changes in the available light spectrum at a physiological and genetic …

Female Reproductive Toxicity Of Harmful Algal Blooms-Produced Toxin Microcystin, Yingzheng Wang

Theses and Dissertations

Harmful algal blooms (HABs) have become a global emerging environmental concern; however, its impact on women’s reproductive health is poorly understood. Microcystin-LR (MC-LR) is the most common and hazardous type of HAB toxin. Using both a chronic oral exposure mouse model and an acute exposure mouse model, I found that human-relevant exposure to MC-LR could compromise follicle ovulation via affecting follicle maturation. Both in vivo mouse exposure model and encapsulated in vitro follicle growth (eIVFG) model revealed that exposure to MC-LR did not affect follicle development, but significantly reduced the expression of some follicle maturation-related genes, such as Lhcgr and …

Molecular Architecture Of Cardiometabolic Responses To Regular Exercise, Jacob L. Barber

Theses and Dissertations

Background: To investigate the molecular mechanisms, determinants, and predictors of cardiometabolic responses to regular exercise, this dissertation had two primary aims: 1) Examine the molecular architecture of cardiometabolic responses to exercise training (unsupervised approach) and 2) Identify molecular signatures of responsiveness to regular exercise within clinically relevant lipoprotein traits (supervised approach).

Methodology: Multi-omics data (17,945 muscle transcripts, 4,979 proteins, 411 metabolites) from participants (n=647) who completed 20 weeks of endurance training as part of the HERITAGE Family Study was collected before and after training. We employed an unsupervised machine learning approach (Aim 1) to identify patterns of molecular responses to …

The Comparative Neuroethology Of Distributed Visual Systems: Visual Neuroanatomy And Visually-Mediated Behaviors Of The Chiton Acanthopleura Granulata And The Bay Scallop Argopecten Irradians, Daniel Ritter Chappell

Theses and Dissertations

Vision is usually thought to be inextricably linked to cephalization; however, certain species of invertebrates deviate from this canonical association by having noncephalized visual systems that are composed of numerous photoreceptive organs distributed across their body paired with centralized, decentralized, or distributed neural architectures. In contrast to cephalized visual systems, we know comparatively little about the visual processing and visually-mediated behaviors of animals with distributed visual systems which are crucial towards understanding how and why these animals see with so many eyes. In this dissertation, we investigate the visual neuroanatomy and visually-mediated behaviors of two different marine molluscs with distributed …

The Effects Of Exercise Training On Cholesterol Efflux Capacity In The Heritage Family Study, Joshua Adam Hawkins

Theses and Dissertations

Cholesterol efflux capacity (CEC) has been associated with cardiovascular disease risk independent of HDL-C levels. However, the effect of regular exercise on CEC is not yet fully understood. Therefore, we examined the effects of exercise training on CEC in the HERITAGE Family Study. In HERITAGE, subjects participated in an endurance training program that consisted of exercise on a cycle ergometer three times per week for 20 weeks. Global and non-ABCA1 CEC were measured at baseline and post-training in 542 subjects via a cell- based radiolabeled CEC assay. Neither global nor non-ABCA1 CEC significantly changed with training in the whole cohort. …

Comparative Assessment Of The Fate And Toxicity Of Chemically And Biologically Synthesized Silver Nanoparticles Toward Juvenile Clams, Mercenaria Mercenaria, Amar Yasser Al-Rshim

Theses and Dissertations

Engineered nanomaterials (ENMs) with unique nanoscale properties, including novel optical behavior and superparamagnetic, are continually being developed for biomedical and industrial applications. In specific biomedical applications, ENMs are surface-functionalized using polymers, proteins, and other stabilizing agents to facilitate their resistance to salt-induced aggregation. Since their colloidal stability in high ionic-strength matrices, functionalized ENMs are anticipated to be persistent aquatic contaminants. Despite their potential environmental significance, the persistence of surface-functionalized ENMs as individually stabilized nanoparticles in marine environments is largely unknown. Further, few studies have investigated the fundamental factors that influence ENMs uptake and fate/transport processes in ecologically susceptible aquatic biota, …

Evaluation Of The Perceived Benefit Of A Psychiatric Resource For Parents Of Children With 22q11.2 Deletion Syndrome, Kayla Blankenship

Theses and Dissertations

The objective of this study was to assess the perceived utility of an educational resource booklet addressing the psychiatric manifestations and mental health considerations within 22q11.2 deletion syndrome (22q11.2DS).

Seventy-three parents of children with 22q11.2DS completed online surveys before and after reading the booklet. The surveys assessed personality factors and feelings of empowerment, worry, self-stigma, and ability to tolerate uncertainty.

Participants reported that the booklet was easy to understand, provided better understanding of 22q11.2DS and mental illness, answered questions about mental illness associated with the condition, improved knowledge of strategies for protecting the mental health of children with the condition, …

Self Reported Cardiovascular Health And Health Behaviors In Women Veterans, Seth Byland

Theses and Dissertations

Objective: The purpose of this study is to describe self-reported cardiovascular health (CVH) and health behaviors in women veterans (WV). A cardiovascular score will be adapted from the existing American Heart Association CVH score. This score will be used to describe CVH in WV.

Methods: Data was collected from WV using an anonymous online survey. This survey asked questions about CVH, CVH behavior, and military specific questions. CVH scoring was determined by asking 6 questions that scored individuals for a total score from 0 to 12, with 12 being most ideal for CVH health. Scores were tallied and mean scores …

Exploring Genetic Counselors’ Experiences, Language, And Discussion Of Consanguinity In Clinical Practice: A Multinational Perspective, Romy Isabel Fawaz

Theses and Dissertations

Consanguinity, defined as the degree of relationship between closely related individuals, is a widespread historical practice that is not specific to any one religion, population, or region of the world. Genetic counselors regularly ask whether a reproductive couple is consanguineous as part of the review of family history and risk assessment. Couples who are in consanguineous relationship may be subject to negative attitudes and stigma, potentially due to cultural differences and norms at a population level and these attitudes may interplay with questions, answers, and discussions around consanguinity. We hypothesized that genetic counselors may experience some level of discomfort discussing …

Psychophysiological Biomarkers Of Concussion Recovery, Adam Todd Harrison

Theses and Dissertations

Each ach year a growing number of individuals report lingering deficits months-years following concussion. Persistent post-concussion symptoms (PPCS) can negatively impact day-today activities and if left untreated may manifest in severe neurological sequelae resulting in long-term cognitive impairment or advanced neurological degeneration (i.e., CTE). Current clinical diagnostic and prognostic assessments (e.g., symptom reports and neurocognitive testing) lack the sensitivity to quantify neurological function. Accordingly, there is a critical need to identify objective biomarkers specific to PPCS to improve an individual’s quality of life and prevent severe long-term neurological dysfunction.

Psychophysiological measurements (e.g., EEG derived event-related potentials, heart rate variability, and …

The Utilization Of Healthcare Chaplains By Genetic Counselors, Elizabeth G. Hollingsworth

Theses and Dissertations

Healthcare chaplains prioritize the spiritual and religious care a patient and their family may need during their healthcare experience. This study investigated the current utilization of healthcare chaplains by genetic counselors, as well as the ability, in time and skill, of the healthcare chaplains to see genetic counseling patients. Lastly, the study investigated if genetic counseling patients would accept a referral to meet with a healthcare chaplain. We hypothesized that genetic counselors are not utilizing healthcare chaplains, healthcare chaplains have the ability to see genetic counseling patients, and genetic counseling patients would consider meeting with a healthcare chaplain.

This study …

Describing The Experiences Of Canadian Genetic Counseling Students Studying In The United States, Madeline Emma Ladouceur

Theses and Dissertations

There are fewer and smaller genetic counseling master’s programs in Canada compared to the United States, which makes it challenging for prospective Canadian students to train in their home country. We investigated which factors influenced Canadian students to apply or not to apply to American genetic counseling training programs, as well as the experiences of those Canadians who attended a program in the United States. We predicted that Canadian students applied to American genetic counseling training programs primarily because of limited training opportunities in Canada and a competitive application process across North America. We used a mixed method, online survey …

Assessing Parental Satisfaction Of Current Fragile X Syndrome Resources Provided At Diagnosis, Megan Michalski

Theses and Dissertations

Diagnosis of a child with fragile X syndrome (FXS) is associated with parental anxiety. Existing literature recommends FXS resources, but there has not been a formal assessment of when parents learn about these resources, or their satisfaction with them. Studies have demonstrated negative feelings surrounding the diagnostic experience of FXS. This exploratory analysis assessed the gap in knowledge on parental satisfaction of the recommended resources from healthcare providers during the time of diagnosis. We predicted parents would not be satisfied with the way resources were presented to them. We discussed parental satisfaction or dissatisfaction, utility of the recommended resources, and …

Examining Parental Disclosure To Unaffected Siblings Of Children Diagnosed With Rett Syndrome, Rachel Nicely

Theses and Dissertations

Rett syndrome is a neurodevelopmental disorder that is inherited in an X-linked dominant manner due to pathogenic variants in the MECP2 gene that are almost always de novo. It is one of the most common causes of developmental delay and intellectual disability in females and occurs in about 1 in 10,000 females. While there is valuable literature surrounding the impact of a neurodevelopmental diagnosis on unaffected siblings, there are no articles about the impact of a disclosure of Rett syndrome to unaffected siblings and the needs of parents during this disclosure. This study aimed to examine the disclosure process …

Understanding The Genomic Influence And Virulence Capabilities Of Environmentally Isolated Vibrios, Shannon Elizabeth Pipes

Theses and Dissertations

The genus Vibrio consists of Gram-negative bacteria that possess a curved rod shape and are routinely isolated from estuarine and coastal salt water. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the major three species that contribute to human disease worldwide, and a variety of other Vibrio species contribute to persistent problems in the aquaculture and fishing industries. The CDC estimates that vibrios cause 80,000 cases of disease each year in the United States alone, however, this number is thought to be underestimated, since some disease is only self-limiting, meaning patients may not seek medical treatment and have …

From Gene Expression To Physiology: A Study Of Chronic Thermal Tolerance In The Mytilus Edulis Species Complex, Lindsey Cate Schwartz

Theses and Dissertations

Rising ocean temperatures are a severe and ever-present threat to marine life. With environmental temperature having such a large impact on organismal performance, understanding the mechanisms which contribute to the ability to survive at higher temperatures is a crucial research focus. Significant progress has been made in discovering these mechanisms on the cellular, biochemical, and physiological levels, but is it much less common for those to be examined together. Using the Mytilus edulisspecies complex as a model system, this dissertation takes a closer look at how prolonged exposure to sub-lethal high temperatures impacts marine organisms on multiple levels of …

Sexual Health Education For Children With Neurodevelopmental Disorders: Genetic Counselor And Pediatrician Perspectives, Mary Catherine Smith

Theses and Dissertations

Many barriers prevent individuals with disabilities from receiving appropriate sexual health education (SHE), including deficits in socialcommunication skills and lack of resources and trained educators. There are few guidelines specifically addressing how to accommodate for providing SHE to individuals with disabilities. Healthcare professionals who are trained in working with adolescents with disabilities, such as pediatric genetic counselors, developmental-behavioral pediatricians (DBP), and neurodevelopmental disabilities (NDD) subspecialty pediatricians, could fill this gap. The purpose of the current study was to assess what informational items pediatric genetic counselors, DBP, and NDD subspecialty pediatricians felt were most essential to include in SHE for children …

Infertility Education For Men With Cystic Fibrosis And Its Effects: Outlooks On Fertility And Birth Control, Allison Elizabeth Perez Szczepanski

Theses and Dissertations

Many men with Cystic Fibrosis (CF) are infertile; however, few studies have evaluated fertility outlook of men with CF since the advent of modulator therapy. As predicted survival and quality of life improve, family planning is an area of increased interest for the CF community. The current study surveyed men with CF to explore their outlook on fertility, family planning, and birth control use with the option to receive a home fertility test and follow-up interview with study staff.

Seventy-five men with CF completed the survey and 53 (71%) believed they were likely infertile. Most recalled learning about infertility between …

The Effects Of Ionizing Radiation On Domestic Dogs: Ancestry And Genetic Structure Of Free-Roaming Dog Populations In Chernobyl, Ukraine, Gabriella Jean Spatola

Theses and Dissertations

This thesis is concerned with the effects of ionizing radiation on domestic dogs (Canis lupus familiaris) which were commonly used as laboratory subjects during the era of atomic bomb testing (1950s - 1980s). Early laboratory studies on radiation effects in dogs provided important foundations of knowledge for research that has more recently shifted to natural populations exposed to ionizing radiation from nuclear accidents. A unique group of free-roaming dogs reside in an environment polluted by radioactive contamination deposited during the Chernobyl nuclear disaster of 1986, providing one such opportunity to study the effects of ionizing radiation on dogs …

Describing The Impact Of The Sales Of Antibiotics On The Environment And In Population Health, Andrés Gaviria-Figueroa

Theses and Dissertations

The role of antibiotics in human health is of significant importance in the advancement of the medical field. It has contributed to an improvement of the quality of life and the age expectancy of the population. Antibiotic resistance is an expected consequence of the use of antibiotics. Bacteria have developed biological mechanisms that would help them become resistant to antibiotics. The uncontrolled use of antibiotics is significantly contributing to the ability of bacteria to become resistant to those antibiotics. This study, using the model of the Socio-Ecological Coupling of Antibiotic Resistance, explains how social and environmental factors interact and impact …

Osteoarthritis And Cartilage Insult: Elucidation Of Molecular Interplay And Attempted Interventions, Brandon James Rose

Theses and Dissertations

Osteoarthritis (OA) is a common and incapacitating joint disease beginning with breakdown of articular cartilage and extending into subchondral bone. At present, the processes through which the disease occurs are poorly understood, and interventions are limited to pain relief and eventual joint replacement. OA is commonly associated with obesity and corresponding pathologies, and as OA is demonstrably not a product of passive erosion of cartilage over time or under increased loads there must needs be some other mechanistic link between the two conditions. We hypothesize that the production of ceramides, a hallmark of the insulin resistance syndrome underlying many obesity-related …

The Use Of Nucleotide Salvage Pathway Enzymes As Suitable Tumor Targets For Antibody-Based And Adoptive Cell Therapies, Edwin J. Velazquez

Theses and Dissertations

Despite the progress made in cancer research, cancer remains one of the leading causes of death worldwide. Although the development of new cancer treatments has improved cancer patients' survival rate, a significant number of patients experience refractory and recurrence events with serious side effects. It is known that the immune system actively participates in eliminating cancer. However, cancer cells can develop mechanisms to evade the immune system resulting in immunotolerance. Immunotherapy aids the patient's immune system's ability to recognize and eliminate cancer cells. During the last three decades, immunotherapy has gradually emerged as an effective and more specific approach to …

The Inhibition Of Valproic Acid-Induced Negative Neurodevelopmental Outcomes Through Nrf2-Mediated Redox Regulation, Ted Brenton Piorczynski

Theses and Dissertations

Valproic acid (VPA) is a commonly prescribed antiepileptic drug that causes negative neurodevelopmental outcomes, including neural tube defects (NTDs) and neurodevelopmental disorders, in fetuses exposed to it. While the exact mechanism by which VPA causes defects is unknown, research has shown that VPA increases the production of reactive oxygen species (ROS) that lead to redox dysregulation. We hypothesize that VPA causes negative neurodevelopmental outcomes through the disruption of redox-sensitive signaling pathways that are critical for proper embryonic development, and that protection from the VPA-induced redox disruption may decrease the prevalence of the observed defects. Time-bred mice were treated with 3H-1,2-dithiole-3-thione …