Life Sciences Commons^™

Increased Healthspan From Exercise, Nicholas Boros

Scholar Week 2016 - present

In this talk we discuss important factors for increased healthspan. Much of the discussion will focus on strength training and cardiovascular training. In particular we outline optimal ways to increase strength and VO₂ max, which are the largest contributors to increased healthspan. We will also explain what makes a strength training program “optimal” mathematically.

Summer 2024 Research Proposal: Swanberg Sanctuary Prairie Plant Community Characterization And Management Testing, Jillian Becksfort

Scholar Week 2016 - present

Prairie management is an involved process that is focused on maintaining existing grassland plant and animal communities. Invasive species and the spread of woody shrubs and trees presents a real threat to the existing prairie. Woody stems can affect resources such as water access and nutrient availability, which may cause changes to the prairie’s unique plant communities. Management techniques like burning and mowing are commonly used to prevent woody stems from causing changes to the prairie ecosystem. The Sanctuary was restored to natural prairie habitat in 2008 and a list of planted species is available. However, no plant surveys have …

Water Lily Leaf Beetle Ecology On Hourglass Lake In Big Lake, Alaska, Haley Lloyd, Grace Beatty

Scholar Week 2016 - present

The Water-Lily Leaf beetle, Galerucella nymphaeae, is a beetle commonly found on Western Pond Lily pads, Nuphar lutea, in Hourglass Lake. Hourglass Lake is located in Big Lake, Alaska. This area is surrounded by boreal forest and bogs and is situated roughly twenty miles North of Anchorage. Within this ecosystem, the Water-Lily Leaf Beetle and the Western Pond Lily are intrinsically linked, as the lily pads serve as a site of feeding and reproduction for the beetles. Dr. Derek Rosenberger of Olivet Nazarene University noticed an abundance of these beetles as he was kayaking through Hourglass Lake, and a project …

Comparing Pollinator Communities: Onu’S Swanberg Sanctuary V. Midewin National Tallgrass Prairie, Stephanie Clark

Scholar Week 2016 - present

Pollinators play a key role in both ecosystem diversity and agricultural productivity [1]. Declines for pollinators have been seen across the globe and have been linked to agriculture and urbanization [2]. Several species in Illinois have declined so severely, they are now endangered or of significant conservation concern [1]. Past ONU students have collected data on the bees in Northeast IL. However, there is no data for what species are at the Sanctuary. This study will provide baseline data for future studies and by comparing with Midewin, a mature restoration, we can gain an understanding of distinctiveness.

A Comparative Study Of Butterfly And Bumblebee Communities At The Onu Prairie And Perry Farm, Kathryn S. Bell

Scholar Week 2016 - present

The decline in many pollinator communities has been a topic of great concern for many years. The primary causes of these declines are habitat loss from urbanization and agriculture, pesticide usage, and climate change. Surveys of pollinator populations are important because they can help determine the health of the pollinator communities. No systematic surveys have assessed the pollinator populations at Olivet Nazarene University’s Prairie or at the LaGesse Prairie at Perry Farm Park in Bourbonnais Township, IL. Thus, little is known about the abundance or the species richness of bumble bees or butterflies in these areas. To address this lack …

School Of Stem Poster Session, Keith Schimmel

Scholar Week 2016 - present

Location: Reed 2^nd floor Tech Center and lobby

This poster session will include displays of work from the School of STEM - Engineering Senior Design and Freshman Design Projects along with Undergraduate Research.

Senior Design Projects

(1) Holland - Shear-Die [Nathan Marks, Nolan Paape, Seth Beyer]

(2) Aginno - Solar-Powered Fish Pond Aeration System [Hoai Do, Bella Lopez, Kendyl Clark, Megan Schroeder]

(3) Peddinghaus - Tube Conveyor [Carson Caldwell, Alisha Wright, Michael Rollberg, Rebecca Witvoet]

(4) American Institute of Chemical Engineers (AIChE) Student Design Problem [Marissa Anderson, Brady Chambers, Cam Steele]

(5) Kankakee Elks Country Club and Golf …

Analysis Of The Population Structure And Migration Habits Of The Northern Leopard Frog (Lithobates Pipiens) At Midewin National Tallgrass Prairie, Gretchen A. Brinkman

Scholar Week 2016 - present

Massive amphibian declines of recent years have pushed researchers to pursue population genetics surveys and to assess the status of these essential components of many ecosystems. The Northern Leopard Frog (Lithobates pipiens) has been continuously experiencing population declines across the continental United States due to a combination of habitat loss and environmental change. Midewin National Tallgrass Prairie houses a considerable portion of Northern Leopard Frog (L. pipiens) in Illinois, and within this location, two creeks provide ideal conditions to support these animals. Because this prairie restoration project is a relatively recent development, further assessments on the …

The Effect Of Education On Support For Tnr As A Method Of Feral Cat Management, Katie Bishop

Scholar Week 2016 - present

A feral cat is a cat that is untamed and not suitable for placement in a home. Through their naturally high rate of reproduction and lack of population management, feral cat populations have grown exponentially leading to overpopulation. Feral cats effect their communities through their impact on wildlife, financial burden, and health risk to both humans and other animals. Due to the impact feral cats place on their communities, multiple management methods have been suggested. Trap-Neuter-Return (TNR) is a humane, effective method of management; however, controversy surrounds the use of this method. Existing studies have determined that things like age, …

The Use Of Aloe-Based Silver Nanoparticles As A Possible Treatment For Contaminated Water, Lozetta Davis

Scholar Week 2016 - present

Water contamination is a world-wide issue that causes the death of millions yearly. This comes from regularly tainted water with water borne pathogens, which is typically seen in undeveloped areas. As antibiotics are used more, more resistance is being seen, and thus newer methods of microbial contamination need to be developed. Silver nanoparticles have shown success in microbial contamination on petri dishes, but not in a water system. It was hypothesized that if silver nanoparticles were added to bacteria contaminated water they would be able to reduce the amount of bacteria present. Silver nanoparticles that are synthesized from plants such …

How Demographic And Social Factors Correlate With The Perception Of Gluten And Gluten Sensitivity (Gs), Grace White

Scholar Week 2016 - present

Learning Objective: To determine which of the presented demographic and social factors correlate with GS or gluten misconceptions to discover potential contributors of GS.

Background: With an increase in GS cases, an exploration and understanding of how demographic and social factors such as gender, ethnicity, economic status, family/friend behavior, and social media correlate with GS and the perception of gluten was sought after.

Method: The researcher constructed a survey that was reviewed by 13 individuals for content, clarity, and internal consistency. The survey was sent out to the student body (2,658) at a small Midwestern university. The final …

The Impact Of Food Prices On College Students' Food Choices, Caroline Boehne

Scholar Week 2016 - present

Learning Outcome: Recognize the need for nutrition education among college students as it relates to health-conscious food purchases and the effect that price of nutrient-dense foods has on college students’ food purchases.

Introduction: Mulik & Haynes-Maslow (2019), noted purchasing healthy foods is often more expensive than unhealthy counterparts. The inability to afford nutrient-dense foods may lead to future health complications. Research results support the need for nutrition education for college students.

Methods: A quantitative online survey was administered to 2,658 college students, n=133. The survey combined Croker’s (2016) General Nutrition Knowledge Questionnaire to evaluate students’ understanding of nutrition and …

Impact Analysis On The Invasive Oriental Weather Loach (Misgurnus Anguillicaudatus) In The Grant Creek And Prairie Creek Watersheds At Midewin National Tallgrass Prairie, Adam Vanhaitsma

Scholar Week 2016 - present

Presentation Location: Weber Center, Room 101

Abstract

In 2014, the invasive Oriental weather loach was found in the Prairie Creek wetland at Midewin National Tallgrass Prairie. While little is known about the Oriental weather loach and its impact on freshwater ecosystems it has become a widespread invasive species. Being the first to investigate the impact of the Oriental weather loach on the Grant Creek and Prairie Creek watersheds at Midewin National Tallgrass Prairie, we set traps for specimens from early June till late July in both watersheds. Each loach that was caught was euthanized and dissected in order to understand …

Mediation Of The Uncoupled Enos Pathway Following Oxidative Stress Using Tetrahydrobiopterin And Nitric Oxide Donor Drugs To Restore Tetrahydrobiopterin Concentration, Brianna Munnich

Scholar Week 2016 - present

Presentation Location: Warming House, Olivet Nazarene University

Abstract

The eNOS pathway, found in the endothelium of blood vessels, is a key regulator of nitric oxide levels in the circulatory system. The pathway is controlled through several positive and negative feedback loops [2]. The cofactor tetrahydrobiopterin (BH4) is a major control point in this pathway and under conditions of stress can be reduced into the dihydrobiopterin (BH2) [2,6,7,8,9]. When the reduced form is predominant, the pathway produces reactive oxygen species (ROS) rather than nitric oxide, causing stress and damage to the vessels [6,7,8,9]. In this study, different treatments were studied …

Surveys Of Bees At Midewin National Tallgrass Prairie With Special Focus On The Possible Presence Of A Rusty Patched Bumble Bee Populations, Nicole Dede

Scholar Week 2016 - present

Native bees are one of the most diverse and important groups of pollinators in many ecosystems. However, pollinators are declining around the world and bumble bees (Bombus sp.) have been significantly affected. Prairie grasslands like those at Midewin National Tallgrass Prairie (Wilmington, IL) are likely prime locations for many species of bees due to rich and consistent floral resources. An important example is the rusty patched bumble bee, B. affinis, which was found at Midewin in 2018. B. affinis is federally listed as a critically endangered species that has experienced a 90% range reduction since 2000. This study is meant …

Changes To The Elevational Distribution Of Craugastor Podiciferus In A Costa Rican Cloud Forest, Tanner Senti, Dakotah Henn, Derek W. Rosenberger

Scholar Week 2016 - present

Presentation Location: Warming House, Olivet Nazarene University

Abstract

While a warming climate is responsible for many animal ranges shifting to higher latitudes and elevations, species will be affected in different ways. High elevation species in the tropics are particularly at risk due to shrinking habitat on mountain tops. Craugastor podiciferus is a cryptic species of flesh-bellied frog native to the Talamanca mountain ranges of Costa Rica and Panama. These frogs inhabit montane cloud forests at an elevational range of 1,090 - 2650 meters. However, they are poorly understood and little is known about the natural history of this species. …

Measuring The Decrement Times Of Volatile Anesthetics In Drosophila Melanogaster Using Gas Chromatography-Mass Spectrometry, Jason Tolley

Scholar Week 2016 - present

Presentation Location: Warming House, Olivet Nazarene University

Abstract

Background

Model organisms are widely used in research, especially in the context of complex situations. One model organism that has been widely used is the common fruit fly, Drosophila melanogaster (D. mel). D. mel are most commonly used in the context of genetics, but they have also been widely used in research focusing on general anesthetics. One value that has not been measured in D. mel, however, as it relates to general anesthetics, is the decrement times. This is what was the present research set out to determine. …

Reconstitution Efficiency On Elution Process, Brandon Winter, Landon Beachy, Samantha Stejskal, Zachary Rice

Scholar Week 2016 - present

Our group is presenting on the Reconstitution Efficiency on Elution Process for MilliporeSigma. The process that we are working with is the mixing of BAAP (Bovine Albumin Acetone Powder) into reverse osmosis water. This process is the first step of many in the making of medical test kits, of which include HIV/AID, strep throat, and pregnancy tests. Our initial task was to reduce the mixing time by 50%, however after further exploration of the problem, a more stable and consistent process turned into our primary goal and a significant reduction in time became a secondary goal. Initially, we were using …

Computational Chemistry - Ulk 101, Michaela Montpas

Scholar Week 2016 - present

Autophagy is a process that generates the necessary building components for cells by cytoplasmic breakdown of unnecessary materials (Martin, Celano, Solitro, Gunaydin, Scott, et. al., 2018). This is a survival technique for cells in times of stress, especially during periods of nutrient starvation. Cancer cells, unfortunately, benefit from this process due to their ability to flourish in nutrient-starved environments, becoming resistant to therapy. The primary protein in mammals responsible for this process is a serine/threonine kinase called ULK 1 (unc-51 like autophagy initiating kinase 1). As such, inhibitors of ULK 1 can be used in cancer therapies in order to …

Hexavalent Chromium: Elucidating Its Carcinogenic Mechanism, And Testing Potential Preventative Treatments, Ryan Himes, Timothy Mayotte

Scholar Week 2016 - present

Cr(VI) (hexavalent chromium) has recently been found in the drinking water of over 250 million Americans. It is a powerful oxidizing agent, and is known to cause cancer, although the specific mechanism has yet to be elucidated. There is no known preventative treatment for Cr(VI) exposure, and the US EPA is currently determining what concentration of Cr(VI) in drinking water can be safely tolerated. This study sought to test the hypothesis that Cr(VI) cytotoxicity can be prevented by various antioxidants. We tested this hypothesis by exposing human embryonic kidney and human intestinal epithelial cells to Cr(VI), with and without the …

Food Preferences Of Red-Headed Woodpeckers (Melanerpes Erythrocephalus) And The Relationship With Season Change, Crystal Sauder

Scholar Week 2016 - present

Red-headed woodpeckers (Melanerpes erythrocephalus) used to be easy to find out in the wild, but as humans encroached on their habitats, their populations started to become impacted. They have a habitat range from Southern Canada down to the Gulf Coast. In several states their numbers are threatened due to loss of habitat. Red-headed woodpeckers prefer to live in open woodlands with dead, or dying, trees to nest in. As humans take away the dead trees from the forests, red-headed woodpeckers are losing their nesting sites. Preservation of red-headed woodpecker habitats are needed to ensure the population starts to …

Dominance And Aggression Among Hummingbirds In The Cloud Forests Of The Talamanca Mountains, Costa Rica, Crystal Sauder, Aileen Scarim

Scholar Week 2016 - present

Hummingbirds (Trochilidae) are known for being aggressive and territorial. By knowing hummingbirds are not very social, nor appear to enjoy having interactions with others, we wanted to observe how multiple species of Trochilidae interact with one another. The study was completed to observe if males of different Trochilidae would be more aggressive at hummingbird feeders over females. Three different feeder locations were decided on, spread across the Savegre Valley region near the Quetzal Education Research Center (QERC). Symbols were agreed upon in order to shorthand the exact series of events that were happening while we observed the feeders. Species and …

Alpha Mangostin As A Chemoprotective Agent Via Activation Of The P53 Pathway For Breast Cancer, Vanessa Van Oost

Scholar Week 2016 - present

Breast carcinoma is the most frequently diagnosed cancer among women and causes over 400,000 deaths yearly worldwide. Current treatments such as chemotherapy are not selective for cancerous tissues but are destructive to normal tissues as well. This causes a range of side effects including pain, nausea, hair loss, weakness, and more. Inactivation of p53 is an almost universal mutation within human cancer cells. The ability to activate the p53 pathway which protects cells from tumor formation is lost in 50% of cancers. Due to the prevalence of this mutation, p53 is a uniquely valuable target for applied research. Alpha mangostin …

The Effects Of Habitat Composition On Red-Headed Woodpecker (Melanerpes Erythrocephalus L.) Population Density, Kimberly Zralka

Scholar Week 2016 - present

Declines in animal populations worldwide are of critical conservation concern. However, without an understanding of optimal habitat preference, it is often difficult to determine what factors are driving these losses. Red-headed woodpecker (Melanerpes erythrocephalus L.) populations have declined by over 70% in the last 50 years, and yet in some areas the birds seem to maintain stable populations. The aim of this study was to empirically test the effects of various habitat factors on red-headed woodpecker abundance. We were particularly interested in whether oak species (Quercus sp.) present in largely savanna environments (an endangered ecosystem in the Midwestern …

The Savegre: Completing The Case Study, Aggie R. Veld, Emilie R. Janes

Scholar Week 2016 - present

Since case studies garner the interest of students necessary for engagement in general biology classes (Himschoot, 2012), the purpose of this project was to create a case study based on the history of San Gerardo de Dota, Costa Rica and Leo and Zana Finkenbinder’s involvement in its transition to sustainability. Travel to Costa Rica occurred in the summer of 2015. Authors Aggie Veld and Emilie Janes presented the story of the case study and progress to date during Honors Week 2016. Since then, the case study was submitted to SUNY Buffalo’s National Center for Case Study Teaching in Science. Feedback …

Site-Directed Mutagenesis Of Malate Dehydrogenase: A Class Project, Bruce J. Heyen, Chesley Rowlett, Jon Zatorski, Ryan Burch, Emily Veach, Andy Gemmaka

Scholar Week 2016 - present

Malate dehydrogenase (MDH) is an important enzyme in an organism’s metabolic pathways. MDH is found in almost all living cells and catalyzes the conversion of malate to oxaloacetate which also involves nicotinamide dehydrogenase (NAD) as a coenzyme. A method to study how an enzyme operates is to alter one of its amino acids and compare the activity of the enzyme before and after the mutation. As a class project in Advanced Biochemistry during the spring semester of 2018, we are working as a team to propose and carry out a point-based mutation on MDH.

Effect Of An Arginine-To-Isoleucine Active Site Mutation On Escherichia Coli Malate Dehydrogenase Enzymatic Activity, Jon Zatorski, Bruce J. Heyen

Scholar Week 2016 - present

Citric acid cycle enzymes function in an environment with numerous substrate analogues and therefore contain active site residue organizations that confer high substrate specificity. Extensive research into the catalytic mechanism of Escherichia coli malate dehydrogenase (eMDH) has identified arginine81 as being crucial to catalysis. In this investigation, an engineered eMDH having an Ile81 rather than an Arg81 (R81I) was isolated using a hexahistadine (His6) tag. Enzymatic activity of the R81I mutant with respect to malate, lactate, and pyruvate was explored. The R81I mutant did show significant activity toward malate, but did not show significant activity toward lactate or pyruvate. Investigations …

Synthesis And Incorporation Of 1,2-Alkanolamine-Functionalized Lysine As A Non-Canonical Amino Acid Into Gfp, Chesley M. Rowlett

Scholar Week 2016 - present

Synthesis of specific post translational modifications in proteins can be difficult but achievable via genetic code expansion techniques. An attempt has been made to synthesize and incorporate D-cThrK into green fluorescent protein (GFP) at an amber mutation site in Escherichia coli via the coordination of pyrrolysyl- tRNA synthetase and its cognate tRNApyl. The incorporation of this non-canonical amino acid and potential chemical transformations following it allow the synthesis of proteins with post translational lysine modifications, making a variety of basic and biotechnological applications available.

The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee

Scholar Week 2016 - present

The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella Typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too …

Physical Activity Monitoring Through A Wearable Technological Device, Alissa Underhill

Scholar Week 2016 - present

PURPOSE: To assess the use of a wearable technological device for the increase in attainment of physical activity (PA) with the goal of preventing Type II Diabetes Mellitus (T2DM) through weight loss.

METHODS: Men and women over the age of 40, and at risk for prediabetes, were recruited. The study period was four months in length, with an initial four weeks of baseline PA testing, followed by 12 weeks of lifestyle intervention. Participants averaged 72 days of PA tracking with 46% of participants reaching the maximal number of days (77). RESULTS: Participants (N = 13) were aged …

Innovative Creation Of A Laboratory Exercise: Fluorescent Staining Of Primary Chicken Embryo Fibroblast Culture Using Mitotracker And Hoechst Stains., Dwight Ginn

Scholar Week 2016 - present

Primary chicken embryo fibroblast cell cultures have been grown in the Developmental Biology course laboratory for many years. With the acquisition of a fluorescent microscope in the Biological Sciences department, it was possible to create a new laboratory exercise incorporating fluorescent staining of these cells. During the fall 2016 semester, primary chicken embryo fibroblast cell cultures were generated from 14 day old chicken embryos, then grown in EMEM with 10% FBS. Living cells were first treated with MitoTracker Orange CMTMRos that stains the mitochondria fluorescent orange. Cells were fixed and permeabilized, then counterstained with Hoechst 33342 DNA stain which stains …