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Full-Text Articles in Life Sciences

Perceptions Of Available Funding Opportunities For West Tennessee’S Young And Beginning Row Crop Farmers, Logan Meeks Jan 2024

Perceptions Of Available Funding Opportunities For West Tennessee’S Young And Beginning Row Crop Farmers, Logan Meeks

Murray State Theses and Dissertations

Research quickly identified many different avenues of funding available to new and beginning traditional row-crop farmers in Tennessee, but questions surrounding local farmers’ knowledge of these opportunities are in question. Many programs specific to the New and Beginning Farmer demographic boasted the potential benefits of using these programs as well as the good work these programs are accomplishing. The current increase in cost of traditional row-crop funding continues to drive the need for more funding resources with New and Beginning Farmers. Providing resources of where farmers can find the best available funds for their operation will expand the level of …


Comparison Of Corticosterone Concentrations Between Serum And Fecal Samples As A Measure Of Stress In Male Broilers., Kayla Weinacht Jan 2023

Comparison Of Corticosterone Concentrations Between Serum And Fecal Samples As A Measure Of Stress In Male Broilers., Kayla Weinacht

Murray State Theses and Dissertations

Stress has long been a topic of interest in the poultry industry due to its potential negative effects on chicken health and production of both eggs and meat. With increased global growth in egg and broiler production, more attention has been paid to the stressors that affect chickens in modern poultry operations, particularly environmental temperature and stocking density. Improving quantitative measurement of stress is always needed in poultry welfare research. In most studies, blood serum is measured for corticosterone, one of the biomarker hormones associated with stress in chickens. Methods of collecting blood serum are by their nature disruptive to …


The Role Of Glis3 In Zebrafish Endocrine Development And Regeneration, Tasha Swenney Jan 2022

The Role Of Glis3 In Zebrafish Endocrine Development And Regeneration, Tasha Swenney

Murray State Theses and Dissertations

Maintenance of proper blood glucose homeostasis is governed by the endocrine cells of the pancreas. Dysregulation of blood glucose is characteristic of the disease, diabetes mellitus. Relatively little is known about the molecular mechanisms that underlie diabetes. The genes associated with more common forms of diabetes remain more enigmatic and likely have less direct roles in blood glucose homeostasis such as glucose sensing, insulin secretion, and β cell maintenance. GLIS3, a transcription factor involved in pancreatic development as well as the transcriptional regulation of insulin has been identified as a risk locus for type 1 and type 2 diabetes …


Effects Of Cannabinoid Supplementation On Weaning And Transportation Stress In Beef Calves, Savannah Kaitlyn Austin Jan 2022

Effects Of Cannabinoid Supplementation On Weaning And Transportation Stress In Beef Calves, Savannah Kaitlyn Austin

Murray State Theses and Dissertations

Transportation stress is a major factor increasing the risk of bovine respiratory disease (BRD) in young calves. Bovine respiratory disease is currently the most economically important disease affecting the cattle industry, costing producers millions of dollars annually. Cannabidiol (CBD) has shown therapeutic benefits in other species, making it a potential tool to use as a supplement to reduce transportation and weaning stress in calves. However, little information is available on cannabinoid bioavailability and disposition of bioactive residue in livestock tissues. The objectives of these studies were to evaluate effective dosage rates of a cannabinoid gel on beef cattle and to …


Effects Of Covid-19 Mandates On Student Engagement And Management Of Collegiate Equestrian Teams, Travis Fortune Jan 2022

Effects Of Covid-19 Mandates On Student Engagement And Management Of Collegiate Equestrian Teams, Travis Fortune

Murray State Theses and Dissertations

Events like a global pandemic can negatively impact how organizations and groups function. From a collegiate perspective, this could include limiting or eliminating participation in co-curricular activities that have positive impacts on student experiences. Students use these events to make new friends, socialize and generate a sense of belonging, and gain interactions outside the classroom. In March 2020, a number of mandated restrictions were put into place in the United States in response to COVID-19, and collegiate equestrian teams' ability to practice and show were negatively impacted. Even though some restrictions were beginning to ease as of Fall 2021, many …


Bottom-Up Effects Of Degradation Outweighs Stabilizing Potential Of Omnivores, Hannah Marie Moore Jan 2022

Bottom-Up Effects Of Degradation Outweighs Stabilizing Potential Of Omnivores, Hannah Marie Moore

Murray State Theses and Dissertations

Anthropogenic activities have led to degradation in streams throughout much of Western North America. In particular, cattle grazing has led to a loss of riparian vegetation resulting in higher water temperatures and an increase in nutrient runoff. The effects of degradation on food quality and quantity for aquatic consumers could have large implications for stream communities. Since omnivores feed at multiple trophic levels, they may be resilient to altered food webs, which may allow them to stabilize communities in degraded environments where resources have been reduced. To test the hypothesis that omnivores positively impact community stability in degraded conditions, I …


Return Of Floodplain Connectivity And Concurrent Macroinvertebrate Community Response Following Wetland Restoration In Western Kentucky, Christiane M. Soldo Ms. Jan 2022

Return Of Floodplain Connectivity And Concurrent Macroinvertebrate Community Response Following Wetland Restoration In Western Kentucky, Christiane M. Soldo Ms.

Murray State Theses and Dissertations

Wetlands improve the quality of our nation’s streams, rivers, and lakes, and they support a diverse assemblage of plant and animal species. The U.S. Department of Agriculture Natural Resources Conservation Service (USDA-NRCS) is responsible for administering the Wetland Reserve Program (WRP), a multi-billion-dollar effort to restore wetlands throughout the nation. Each year, WRP enrolls thousands of acres of private farmland into conservation easements with the goal of improving water quality and creating wildlife habitat. Hydrological modification structures, such as levee breaks, ditch plugs, or shallow water areas are constructed on easements to create wetlands by improving water retention and returning …


Fish Communities Respond To Hydrology And Elevation In Restored Western Kentucky Wetlands, Lucas Zuklic Jan 2022

Fish Communities Respond To Hydrology And Elevation In Restored Western Kentucky Wetlands, Lucas Zuklic

Murray State Theses and Dissertations

The Wetlands Reserve Program (WRP) is designed to restore wetland ecological function and wildlife benefits; however, these projects rarely monitor biological responses. The objectives of our study were to (1) identify environmental factors that were the most important in determining wetland fish community composition, (2) examine the influence of the environment on specific groups of fish (large-river fishes and Kentucky Species of Greatest Conservation Need (KY-SGCN) wetland fishes), and (3) compare fish community composition and diversity in wetlands of different condition (e.g., degraded, restored, and reference). From April 2019 to August 2020, monthly electrofishing surveys were performed in degraded …


Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg Jan 2021

Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg

Murray State Theses and Dissertations

The differences in energetic input between the sexes required to produce gametes translates to the differences in reproductive behavior and overall mating systems seen in a species. Females generally produce a few energetically and resource expensive eggs and typically choose a high-quality suitor to ensure she has high quality offspring. In contrast, males produce abundant energetically cheap sperm and attempt to fertilize as many eggs as possible in as many females as possible. Both sexes are trying to maximize their inclusive fitness, but the dichotomy of interests can lead to sexual conflict and perhaps extreme or unusual behaviors such as …


Characterizing The Host Usage And Development Of Ormia Lineifrons (Diptera: Tachinidae), Kyler J. Rogers Jan 2021

Characterizing The Host Usage And Development Of Ormia Lineifrons (Diptera: Tachinidae), Kyler J. Rogers

Murray State Theses and Dissertations

Insect communication systems are strongly driven by the evolution of signals or signal preferences. These systems rely on a signaler to truthfully emit signals for receivers to interpret. Often, these signals are conspicuously broadcasted. Conspicuous signals involved in animal communication are intended to attract mates, however, these signals are often exploited by eavesdroppers. Thus, many communication systems experience natural selection and sexual selection acting in opposite directions. New adaptations can arise in response to selective pressures, such as eavesdroppers, leading to co-evolving systems between eavesdroppers and hosts, for example. Understanding these systems can provide valuable insight into how unintended receivers …


Glial Granules And Germline Proteins In The Drosophila Brain, Samuel J. Tindell Jan 2021

Glial Granules And Germline Proteins In The Drosophila Brain, Samuel J. Tindell

Murray State Theses and Dissertations

My overarching goal was to elucidate the role of Tudor (Tud) protein in the Drosophila brain. Tud is an evolutionarily conserved molecular scaffold, containing 11 Tud domains which bind to symmetrically dimethylated arginine (sDMA) residues in a variety of proteins. Tud is an essential component of embryonic ribonucleoprotein complexes (RNPs) called germ granules that contain RNAs and proteins crucial to the differentiation and maintenance of germ cells, the specialized class of stem cells that ultimately give rise to the next generation of organism. In the absence of Tud, there is a complete abolishment of germ cells highlighting its important role …


Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett Jan 2021

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …


The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik Jan 2021

The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik

Murray State Theses and Dissertations

Hatchery Creek is a restored stream in Jamestown, KY that drains Wolf Creek National Fish Hatchery. The previous degraded channel of Hatchery Creek caused large sediment plumes in the Lower Cumberland River and was restored to decrease sediment loss and provide the opportunity for a self-sustaining trout population. I predicted that the increased amount of habitat would increase taxa richness, abundance and biomass of macroinvertebrates. Macroinvertebrates were monitored in three periods; the degraded period, a one-year recovery period, and a restored period using surber and kicknet samples in order to determine biomass, abundance, diversity, taxa richness, and macroinvertebrate biotic index. …


Dirofilaria Immitis Prevalence In Canis Latrans In Kentucky, Melanie Ann Brandon Jan 2021

Dirofilaria Immitis Prevalence In Canis Latrans In Kentucky, Melanie Ann Brandon

Murray State Theses and Dissertations

Dirofilaria immitis (canine heartworm) was found in forty-two (42) of the two hundred seventy-five (275) Canis latrans (coyote) necropsied in the state of Kentucky from November 27, 2019 through March 3, 2021. Thirty-five (35) of the positive cases were from western Kentucky region with the other seven spread across the state. With this group of coyotes, one hundred fifty-eight (158) were male and the other one hundred six-teen (116) were female. The estimated age ranged from a pup to senior dogs. A little over forty percent of the dogs were obtained through coyote/predator tournaments; the remaining were acquired from pest …


Work Ethic And Productivity: The Mediating Role Of Leadership, Everly Tapp Jan 2021

Work Ethic And Productivity: The Mediating Role Of Leadership, Everly Tapp

Murray State Theses and Dissertations

Employee work ethic and workplace productivity are vital for organizational success. Past research has shown leadership style to be influential on both, with the manager-employee relationship impacting employee’s attitude and output in the workplace. This study investigated the relationship between employee work ethic and workplace productivity, while also examining the impact of transformational, transactional, and laissez-faire leadership styles on agriculture equipment dealership employees. The leadership style of service managers, as measured by the Multifactor Leadership Questionnaire (MLQ) (5x-Short), was compared to the workplace productivity and work ethic scores of the service technicians they manage. Service technician workplace productivity data was …


Evaluating The Effects Of Residual Broiler Litter And Copper In Soil On Hemp (Cannabis Sativa L.) Fertility And Cannabinoid Production, Sarah E. Forden Jan 2021

Evaluating The Effects Of Residual Broiler Litter And Copper In Soil On Hemp (Cannabis Sativa L.) Fertility And Cannabinoid Production, Sarah E. Forden

Murray State Theses and Dissertations

This study encases two separate experiments observing hemp (Cannabis sativa L.) and soil fertility with the use of broiler litter and copper. The long-term broiler litter research plots were established in 1997 and the copper fertility research plots were not established until 2009. Both experiments use a randomized complete lock design with the broiler litter experiment composed of four replications and eight treatments at the following rates: 0, 1, 2, 3, 4, 5, 6, and 7 tons BL * ac-1. The copper experiment was designed with four replications and three treatments at the following rates: 0, 10, …


An Evaluation Of College Preparedness On Natural Selection Principles Following The Completion Of An Entry-Level Biology Course, Amy Bell Jan 2021

An Evaluation Of College Preparedness On Natural Selection Principles Following The Completion Of An Entry-Level Biology Course, Amy Bell

Murray State Theses and Dissertations

Abstract

Evolution education in secondary education has long been a topic of research. The level of knowledge and acceptance of students upon entering college has been studied using various methods; however, no study had provided the perception of preparedness from the student perspective nor had analyzed the individual Natural Selection principles. This study analyzed college freshmen (n=162) in an entry-level BIO 101 course. Participants were given the Conceptual Inventory of Natural Selection Instrument (CINSI) and perception survey questions upon completion of the course. The CINSI data was then analyzed for each of the four Natural Selection principles as well as …


Effectiveness Of The Two Plus Two Online Program In Agriscience Education: A Case Study Of Students’ Experiences And Persistence, Ronald M. Howell Jr Jan 2021

Effectiveness Of The Two Plus Two Online Program In Agriscience Education: A Case Study Of Students’ Experiences And Persistence, Ronald M. Howell Jr

Murray State Theses and Dissertations

Higher education is changing, and more community colleges find themselves helping to increase the number of students who receive a bachelor’s credential from a 4-year post-secondary institution. This qualitative study was designed to determine the effectiveness of the online two plus two Agriscience Education program at a university in North Carolina. Twelve current and former students provided feedback about their perspectives and lived experiences through semi-structured interviews.

Preliminary findings suggest that both current and former students find the two plus two program very cost-affordable, flexible, and convenient for transferring two years’ worth of credit and courses to earn a bachelor’s …


Impact Of Teacher Training Programs In Georgia On The Self-Efficacy Of Agricultural Education Teachers In Their First Year, Catrina Pollard, Catrina Pollard Jan 2020

Impact Of Teacher Training Programs In Georgia On The Self-Efficacy Of Agricultural Education Teachers In Their First Year, Catrina Pollard, Catrina Pollard

Murray State Theses and Dissertations

Teacher self-efficacy is directly tied to teacher longevity. The researcher conducted a causal comparative study to determine self-efficacy in three domains: classroom, FFA, SAE and compared them with demographic characteristics, along with where teachers obtained their teacher training/certification. The purpose of this study was to determine the relationship between agricultural teacher self-efficacy and the teacher preparation programs that they completed. This surveyed first-year teachers within the state of Georgia that teach agricultural education. The Beginning Agriculture Teacher Assessment determined that there was no connection between teacher self-efficacy and the teacher preparation program. The Beginning Agriculture Teacher Assessment did conclude that …


Population Demographics Of Silver Carp Hypophthalmichthys Molitrix In Kentucky Lake, Allison M. Lebeda Jan 2020

Population Demographics Of Silver Carp Hypophthalmichthys Molitrix In Kentucky Lake, Allison M. Lebeda

Murray State Theses and Dissertations

Invasive species continue to threaten aquatic ecosystems in the United States. Silver Carp Hypophthalmichthys molitrix have successfully infiltrated much of the Mississippi River Basin, including Kentucky Lake – a large reservoir located on the Tennessee River in Western Kentucky. Although Silver Carp have been present in Kentucky Lake for at least a decade, until recently, very little was known about the population, how often successful reproduction is occurring, or the environmental conditions that facilitate strong year-classes. Hence, it is difficult for managers to predict the potential impact of Silver Carp on native species. Silver Carp were collected from Kentucky Lake …


Incorporation Of Hempseed In The Broiler Chicken Diet, Mallorie Alexzandra Snider Jan 2020

Incorporation Of Hempseed In The Broiler Chicken Diet, Mallorie Alexzandra Snider

Murray State Theses and Dissertations

Hempseed has become a popular supplement alternative due to the confounding nutritional benefits it possesses; however, the legality of hempseed, and other hemp products, prevents the use in animal feeds. Particularly, broiler chickens that grow fairly quickly in a short amount of time. Evaluation of hempseed in the broiler chicken diet is needed to conclude if it is a possible replacement for other broiler nutrient sources, such as soybean meal (SBM). Thus, the objective of this study was to evaluate the effects of hempseed (HS) on the growth, feed conversion and carcass yield of broilers. This study consisted of 48 …


Effects Of The Asian Clam, Corbicula Fluminea, On Native Freshwater Mussels And Biofilms, Kiersten Youngquist Jan 2020

Effects Of The Asian Clam, Corbicula Fluminea, On Native Freshwater Mussels And Biofilms, Kiersten Youngquist

Murray State Theses and Dissertations

As native, freshwater mussels continue to decline in the United States, successful conservation and management plans are essential for their survival. Understanding the drivers of native mussel declines, how the ecological roles mussels play within aquatic systems may be affected by declines, and the conservation status of our mussel fauna in different watersheds is critical to inform management and conservation efforts. The Asian clam (Corbicula fluminea, Müller, 1774) is an invasive species found throughout the United States that can reach densities of greater than 1000/m². Given these high densities, Asian clams have been linked to declines in native mussels …


Cannabidiol In The Horse: Pharmacokinetics And Effects Of A Pelleted Supplement On Reactivity And Movement, Anna L. Draeger Jan 2020

Cannabidiol In The Horse: Pharmacokinetics And Effects Of A Pelleted Supplement On Reactivity And Movement, Anna L. Draeger

Murray State Theses and Dissertations

A multitude of claims exist regarding therapeutic benefits of cannabidiol (CBD) in human and animal medicine. Though supportive evidence of CBD as a nutraceutical option exists, lack of regulation means that product safety, consistency, and efficacy cannot be guaranteed. Trials for specific conditions and species are needed. The objective of this study was to evaluate CBD safety and use effects on reactivity and movement in the horse. Project 1 examined the bioavailability of a single 50 mg dose of an oil and pelleted CBD product. One of 2 Quarter Horse geldings received the oil product. The second received the pelleted …


Effect Of Organic Soil Amendment On Hemp, Mounica Talasila Jan 2020

Effect Of Organic Soil Amendment On Hemp, Mounica Talasila

Murray State Theses and Dissertations

The research study is about the impact of different levels of organic soil amendment on the levels of psychoactive compounds produced in industrial hemp. Inorganic or chemical pesticides or fertilizers are not as extensively used in hemp as in other crops. Apart from this, using an organic soil amendment would be an environmentally sustainable way. A randomized block design (RBD) model was used to study the impacts of the soil amendment. The soil amendment used is called “Rehab” a product of Organilock created from the remnants of animals like fish and chicken. This research design has 3 different soil amendment …


A Survey On Horse Use And Management In Horse Clubs In China: A Pilot Study, Zhen Li, Zhen Li Jan 2019

A Survey On Horse Use And Management In Horse Clubs In China: A Pilot Study, Zhen Li, Zhen Li

Murray State Theses and Dissertations

During the past three decades, significant development of the Chinese equine industry has resulted in a rapid increase in the number of horses in the country. At the same time, many horse clubs face shortages of trained management personnel or specialists in equine science and technology. The objective of this survey was to document and evaluate horse management and use in Chinese horse clubs. A 29-question survey was developed using SurveyMonkey© and tested on a small pilot group before being revised and released to a general pool of participants. The questionnaire link was distributed through WeChat to specific personnel in …


Trophic Interactions And Niche Partitioning Within A Post-White-Nose Syndrome Temperate Bat Community, Macy Kailing Jan 2018

Trophic Interactions And Niche Partitioning Within A Post-White-Nose Syndrome Temperate Bat Community, Macy Kailing

Murray State Theses and Dissertations

The decline of cave-dwelling bats since the introduction of white-nose syndrome (WNS) to North America changed the way communities interact. Disease-mediated competition at the community level can influence the ability of imperiled species to recover because of competitive exclusion. In western Kentucky, tri-colored bats, Perimyotis subflavus, which are susceptible to WNS severely declined following WNS occurrence. During that same period, evening bats, Nycticeius humeralis, which are not susceptible to WNS increased markedly. To investigate the influence of WNS on community structure, the diets of sympatric tri-colored and evening bats were assessed. Guano was collected from evening (n=37) and …


Influence Of Soil And Plant-Plant Interactions On Growth And Flowering Of Two Potentially Dominant Bunchgrasses In The Longleaf Pine Ecosystem, James C. Groover Jan 2018

Influence Of Soil And Plant-Plant Interactions On Growth And Flowering Of Two Potentially Dominant Bunchgrasses In The Longleaf Pine Ecosystem, James C. Groover

Murray State Theses and Dissertations

Neighborhood interactions and edaphic conditions can help predict the distribution of species and the composition and structure of plant communities. The longleaf pine ecosystem of the southeastern U.S. provides an ideal setting in which to study interactions among dominant members of the understory community. Bunchgrasses provide the structure and fuel that enable frequent fires to mediate succession and maintain the extremely diverse understory community characteristic of the imperiled longleaf pine ecosystem. I investigated responses to bunchgrass neighborhood composition by wiregrass (Aristida stricta Michx.) and little bluestem (Schizachyrium scoparium [Michx.] Nash), two competing and potentially dominant C …


The Effect Of Three Different Mulches On Weed Presence, Soil Characteristics, And Zinnia Growth, Anmar Muttaleb Jan 2018

The Effect Of Three Different Mulches On Weed Presence, Soil Characteristics, And Zinnia Growth, Anmar Muttaleb

Murray State Theses and Dissertations

Abstract

Organic and inorganic mulching helps to control weeds. Mulching helps cultivated plants to grow by inhibiting the growth of weeds, retaining soil moisture, and regulating the temperature of soil. The objective of this study was to determine the effects of different organic mulches on weed presence, soil characteristics, and growth of Zinnia elegans. The mulches used in studying Zinnia elegans were wheat straw, non-shredded Miscanthus (M. × giganteus), and shredded Miscanthus (M. × giganteus) mulch. A Randomized Complete Block Design (RCBD) was used in the study, with different quantitative methods were used to …


Effects Of Roundup On Behavior, Growth, And Mortality Of Larval Blue Dashers, Pachydiplax Longipennis, Kayleen K. Parker, Claire A. Fuller Jan 2018

Effects Of Roundup On Behavior, Growth, And Mortality Of Larval Blue Dashers, Pachydiplax Longipennis, Kayleen K. Parker, Claire A. Fuller

Murray State Theses and Dissertations

The objective of this study was to determine if Roundup (active ingredient: Glyphosate) causes negative effects on behavior, growth, and mortality of larval Pachydiplax longipennis. Larvae were captured from rainwater-filled mesocosms at Hancock Biological Station in Murray, KY. Larvae were exposed to one of four concentrations of Roundup (0mg/L, 2.5mg/L, 5mg/L, or 10mg/L). Daphnia consumption, seek refuge, and anti-predator trials were conducted at 7 and 14 days post-exposure. Growth and survival trials were carried out for eight weeks using different larvae. There were no significant differences among treatments for whether or not larvae ate offered Daphnia for Day 7 …


Effects Of Gli-Similar 3 Knockout Mutations On The Expression Of Insulin Transcription And Pancreatic Islet Development In Zebrafish, Dylan James Hammrich Jan 2018

Effects Of Gli-Similar 3 Knockout Mutations On The Expression Of Insulin Transcription And Pancreatic Islet Development In Zebrafish, Dylan James Hammrich

Murray State Theses and Dissertations

Blood glucose homeostasis is a critical component in the physiological health of vertebrates. Regulation of blood glucose levels is the responsibility of the endocrine pancreas, which excretes hormones to the bloodstream in response to changes in glucose concentrations. The hormones excreted from the pancreas include insulin, which is secreted form the β cells of the pancreas and signals uptake of excess glucose by the peripheral tissues, and glucagon, which is secreted from the α cells and signals release of glucose to the bloodstream through gluconeogenesis in the liver. The Krüppel-like zinc finger protein Gli-Similar 3 (Glis3) is a transcription factor …