Life Sciences Commons^™

Mitochondrial Mrna Translation Is Required For Maintenance Of Oxidative Capacity, David Lee

Graduate Theses and Dissertations

Oxidative metabolism is required to produce adequate energy to sustain human life. A primary example of deteriorating oxidative capacity is seen in the cardiac musculature during chronic heart failure. This suggests that by improving oxidative potential, chronic heart disease could be mitigated and one approach to accomplish this may be through targeting the mt-mRNA translation system. Purpose: This investigation’s purpose was to characterize disruptions in mt-mRNA translation machinery in multiple forms of cardiomyopathy and to determine if mitochondrial mRNA translation initiation factor (mtIF2) is necessary to maintain oxidative capacity in cardiomyocytes. Methods Using a combination of animal and cell culture …

An Evaluation Of Breeding, Agronomic, And Processing Methodologies Of Vegetable Soybean (Edamame) To Increase Domestic Production In The United States Market, David Octor Moseley

Graduate Theses and Dissertations

Edamame is a food-grade soybean (Glycine max (L.) Merrill) that is either harvested immature (R6 stage) or after plant maturity (R8 stage). At R6, the premium product will have crescent- shaped large green pods and gray pubescence. At R8, the seed will either have stayed green or will have turned yellow, black, or brown. Edamame is a healthy snack with a sweet flavor and firm texture. The edamame market is growing in the United States, creating a need for more adapted varieties. The genetic diversity is low among adapted large-seeded breeding lines. Finding diverse accessions will help develop larger and …

Soil Properties That Influence The Occurrence Of Hydrogen Sulfide Toxicity In Rice Fields, Julia Marie Fryer

Graduate Theses and Dissertations

Rice (Oryza sativa L.) producers face many challenges throughout each growing season. Hydrogen sulfide (H2S) toxicity is a physiological disorder where sulfate (SO42-) is excessively reduced to the toxic gas, H2S. This can reduce yield and, in severe cases, result in crop death. The main research objectives were to: i) understand chemical and physical characteristics in soils prone to H2S toxicity, ii) determine influential soil characteristics on the incidence of H2S toxicity, iii) determine ammonium sulfate ((NH4)2SO4) fertilizer additions influence on H2S toxicity, and iv) predict when and where H2S will occur. Three greenhouse experiments were conducted using Arkansas field …

Effect Of A Skating Unit On Fitness In Fifth Grade Students, Eric Lange

Graduate Theses and Dissertations

The purpose of this quantitative study investigates the effect of a skating unit on factors of cardiovascular fitness, static balance, explosive power, and agility in fifth graders. Methods: During a 6-weeks skating unit (twelve lesson), seventy-one students (ages 10-12; M age = 10.34) participated during regularly scheduled physical education classes. Three separate classes served as control group (C) (n=19; 8 male; 11 female) and two experimental groups: roller skating (RS) (n=26; 11 male; 15 female) and inline skating (IS) (n=26; 10 male; 16 female). Control group had no access to roller or inline skates during class time of study. Control …

State Dependent Function And Dynamics In Cerebral Cortical Networks, Leila Fakhraei

Graduate Theses and Dissertations

Cerebral cortex exhibits vigorous ongoing, internal neural activity even with no sensory input is present or the animal is minimally engaged in a task or behavior. This internal ongoing activity is not static; the ‘cortical state’ varies ranging from synchronous and highly correlated activity to asynchronous and weakly correlated neural activity. The main goal of the work presented here is to understand how changes in cortical states effect several aspects of cortical function and dynamics.

To meet this goal, we did three separate projects. First, we compared the predictability of neuronal network dynamics across cortical states in somatosensory cortex of …

Evaluation Of Dicamba Off-Target Movement And Subsequent Effects On Soybean Offspring, Gordon Travis Jones

Graduate Theses and Dissertations

Commercial launch of cotton with resistance to dicamba, glyphosate, and glufosinate occurred in 2015 and launch of soybean with resistance to dicamba and glyphosate occurred in early 2016. It is likely that non-dicamba-resistant soybean will be planted in close proximity to dicamba-resistant soybean and cotton. Therefore, experiments were conducted to examine the distance dicamba moves during an application using commercial application equipment, as well as the effect the drift events have upon soybean offspring. Additional experiments were designed to investigate the effect glyphosate addition to dicamba has upon soybean growth and yield as well as possible effects on offspring. Lastly, …

Combining Microdialysis And Electrophysiology In Cerebral Cortex To Delineate Functional Implications Of Acetylcholine Gradients, Tazima Nur

Graduate Theses and Dissertations

The neuronal network in cerebral cortex is a dynamic system that can undergo changes in collective neural activity as the organism changes its behavior. For example, during sleep and quiet restful awake state, many neurons tend to fire together in synchrony. In contrast, during alert awake states, firing patterns of neurons tend to be more asynchronous, firing more independently. These changes in population-level synchrony are defined as changes in cortical state. Response to sensory input is state-dependent, i.e., change in cortical state can impact the sensory information processing in cortex and introduce trial-to-trial variability in response to the same repeated …

Towards Quantifying Relevant Land Cover Change: A Case Study In The Central Flyway Of The Monarch Butterfly, Hanna Lenee Ford

Graduate Theses and Dissertations

Over the past 20 years notable decreases in monarch butterfly populations have led researchers to begin evaluating the landscape for changes and seeking out opportunities for enacting conservation programs to better support their survival. The monarch butterfly has recently come under consideration for listing under the Endangered Species Act which has created a need for a more informed view of the landscape through which the migrate and breed, the central United States. In this research three spatially-explicit models are explored using the most applicable datasets currently available to address pressing policy and land manager decisions regarding monarch butterfly and pollinator …

Nitrous Oxide Emissions From Rice Production On A Silt-Loam Soil In Arkansas, Casey Rector

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is a common crop grown in Arkansas under flooded-soil conditions. The saturated to nearly saturated soil makes rice production an ideal environment for the production of potent greenhouse gases, such as nitrous oxide (N2O). The objectives of this study were to i) evaluate the impact of water management practice (full-season-flood and intermittent-flood) and cultivar (pure-line and hybrid) on N2O fluxes, season-long N2O emissions, and global warming potential (GWP; 2016) and ii) evaluate the impact of tillage practice [conventional tillage and no-tillage (NT)] and type of urea fertilizer [ N-(n-butyl) thiosphosphoric triamide (NBPT)-coated and non-coated urea] on …

Antiproliferative Activity Of Carotenoids, Phenolic Compounds, And Volatile Terpenoids In Carrots (Daucus Carota L.), Brittany Frederick

Graduate Theses and Dissertations

Epidemiological studies have shown an association between high carrot consumption and low prevalence of cancer. This observation has been thought to be attributed to carrot carotenoids. Despite this, various intervention trials have displayed no changes in incidence or increased incidence of cancer with carotenoid supplementation. It is possible that carrot phenolics are responsible for this association, though this has not been widely accepted. Volatile terpenoids from carrots have not been studied in this regard. Therefore, the primary objective of this study was to compare the antiproliferative effects of carotenoids, phenolics, and volatile terpenoids extracted from carrots on Caco-2 colon cancer …

Evaluation Of The Efficacy Of A Candidate Turkey Dermatitis/Cellulitis Oil Emulsion Vaccine On Immune Response, Morbidity, And Mortality Under Laboratory And Commercial Conditions, Brittany Danielle Graham

Graduate Theses and Dissertations

Alpha-toxigenic Clostridium septicum (CS), the cause of turkey cellulitis, results in devastating mortality with high costs for the industry. Various vaccinations have been evaluated to prevent this disease with moderate success. Ability of a CS bacterin-toxoid, in conjunction with adjuvants such as aluminum hydroxide, mannoslyated chitosan, or a water-in-oil Seppic Montanide 71 R VG adjuvant (OE) to induce immunity was evaluated in a 7-week study (Experiment 1). Poults (20/group) were vaccinated day-of-hatch, boosted at 5 weeks-of-age and compared to unvaccinated controls. Antibody titers were determined by ELISA for all experiments. In experiment 1, initial vaccination with OE resulted in significantly …

Examining The Appropriate Recovery Interval Following Maximal Exertion For Baseline Computerized Neurocognitive Testing (Cnt), Samantha Mohler

Graduate Theses and Dissertations

Background: Computerized neurocognitive testing is part of the recommended multi-faceted approach to SRC assessment. Prior research has suggested that maximal exertion negatively effects CNT test scores. Purpose: To identify the appropriate timing of the administration of CNT following maximal exertion in healthy college-aged students. Study Design: Random cross-over, repeated measures design. Methods: Participants will be administered CNT on four different visits, with at least one week between administrations. A VO2 max treadmill test will be performed before CNT administration during three of the four trials. Following the VO2 max test, participants will rest for <2 minutes (immediate), 10-minutes, or 20-minutes before taking CNT. The fourth trial, without maximal exertion preceding CNT administration, will serve as the control. All trials will be randomly-counterbalanced to negate practice effects. RESULTS: There was a significant within-subjects effect for prescribed post-exertion recovery intervals on total symptom scores (Wilks λ = .62, F [3, 23] = 4.64, p = .01, η2= .38). Total symptom scores were significantly higher at the immediate (p < .002), 10-minutes (p = .018), and 20-minutes (p = .011) post-exertion recovery intervals compared to baseline. Additionally, a significantly positive within-subjects effect for prescribed post exertion recovery was observed for processing speed (p=.009, Wilks λ = .60, F [3, 27] = 5.9, η2 = .396). No significant effect was observed for visual memory (p = .07), verbal memory (p = .06), or reaction time (p = .40). CONCLUSION: Baseline symptom scores were negatively influenced processing speed was enhanced by maximal exertion. These changes continue to be elevated 20 minutes post-exertion. Moreover, cognitive performance was not significantly impaired following maximal exercise. To obtain more accurate baseline symptom scores, and allow processing speed composites to return to normal, sports medicine professionals should wait at least 20 minutes following maximal exertion before administering CNT.

Bayesian Network Modeling And Inference Of Gwas Catalog, Qiuping Pan

Graduate Theses and Dissertations

Genome-wide association studies (GWASs) have received an increasing attention to understand genotype-phenotype relationships. The Bayesian network has been proposed as a powerful tool for modeling single-nucleotide polymorphism (SNP)-trait associations due to its advantage in addressing the high computational complex and high dimensional problems. Most current works learn the interactions among genotypes and phenotypes from the raw genotype data. However, due to the privacy issue, genotype information is sensitive and should be handled by complying with specific restrictions. In this work, we aim to build Bayesian networks from publicly released GWAS statistics to explicitly reveal the conditional dependency between SNPs and …

The Effect Of Napping On Sleep Quality And Quantity In Healthy And Concussed Collegiate Athletes, Katie Stephenson-Brown

Graduate Theses and Dissertations

Clinicians are increasingly prescribing a regulated sleep schedule to athletes post injury; however, baseline sleep habits of collegiate athletes are unknown. Moreover, the connection between napping and sleep quality and quantity has not been studied in collegiate athletes. Therefore, the purpose of this study is to investigate napping behaviors and their effects on sleep quantity and quality in concussed and non-concussed collegiate athletes. In a sample of 233 non-concussed collegiate athletes, 74% (172/233) of participants reported napping. Napping was not significantly associated with sleep quantity (χ 2 (2) = .23, p = .64) or quality (χ 2 (1) = .42, …

Evaluation Of Fresh-Market Potential Of Arkansas-Grown Fruit: Blackberries, Peaches, Table Grapes, And Muscadine Grapes, Molly Felts

Graduate Theses and Dissertations

Fresh-market produce is a major component of commercial market sales. However, shelf-life of fresh-market produce is limited, so evaluating postharvest potential (time from harvest to consumption) is critical. Fresh-market fruit can be impacted by many factors that deteriorate the quality of the fruit. Understanding the postharvest physiology of fruit can lead to better handling and storage conditions for extended shelf life and enhanced quality for the consumer. This research project was a collaborative effort within the University of Arkansas System Division of Agriculture between the Food Science and Horticulture Departments to evaluate the fresh-market potential of Arkansas-grown fruit. Physiochemical and …

Seasonal Phenology, Distribution And Treatments For Polyphagotarsonemus Latus (Banks) On Primocane-Fruiting Blackberries (Rubus L. Subgenus Rubus) In Arkansas, Jessica Anne Lefors

Graduate Theses and Dissertations

Worldwide, blackberries (Rubus L. subgenus Rubus) are an economically important crop. In 2007, Polyphagotarsonemus latus (Banks) (broad mites), were first reported damaging primocane-fruiting blackberries in Fayetteville, Arkansas. Since this time, broad mite damage to blackberries and yield loss has been reported in many states and countries. Despite the increasing reports of this blackberry pest, little is known about their population dynamics, and few treatments are available. Growers with broad mite populations need a pest management program to minimize yield loss. Therefore, there was a need to develop sampling techniques, describe broad mite seasonal phenology in blackberry fields, and determine efficacy …

The Effect Of Cellular Dehydration On Glucose Regulation In Lean And Obese Females, Chunbo Yang

Graduate Theses and Dissertations

A chronically elevated vasopressin level has been associated with an impaired glucose regulation system in humans. A recent study in patients with diabetes suggested that low water intake is associated with impaired glucose regulation. The aim of this study was to examine the effect of hypertonic saline infusion on glucose regulation in healthy females. Thirty healthy, non-diabetic females performed a 2-h infusion followed by a 4-h oral glucose tolerance test (OGTT). Every subject participated in two trials separated by one month, in a counterbalanced order. Two trials were identical in the procedure, differing in the concentration of saline infusion, with …

Evaluation Of The Effects Of Formaldehyde On Growth Parameters Of Broiler Chicks, Paula Johnson

Graduate Theses and Dissertations

Formaldehyde has been used as a disinfectant in poultry hatching cabinets to aid in controlling key pathogenic organisms, such as Salmonella and Escherichia coli (E.coli). There is some evidence that prolonged exposure of chicks to formaldehyde can reduce tracheal ciliary function and thus reasons to believe that exposure to formaldehyde, in the absence of pathogen challenge, may reduce chick vitality. It has been found that elevated temperatures during incubation may adversely affect body weights of broiler chicks, as well as post-hatch environmental heat stress on performance in poultry. Post-hatch environmental heat stress has been shown to impact performance in poultry. …

Survey Of Hydration Knowledge And Behavior In Youth Mountain Bike Teams, Natasha Brand

Graduate Theses and Dissertations

Many youth sports organizations recognize the importance of educating on hydration practices and awareness, although according to recent research current hydration education is not correlating to effective practices. PURPOSE: The purpose of this study was to assess and describe the hydration knowledge and behavior of student-athletes participating in competitive junior high and high school mountain bike teams, in order to substantiate further research. METHODS: A total of 133 male and female participants, ranging from 11-18 years were surveyed on their hydration knowledge and behavior. All participants were apparently healthy and were active members of a National Interscholastic Cycling Association (NICA) …

The Effects Of Super-Dosing Phytase On Nursery And Grower Pigs, Joshua Paul Knapp

Graduate Theses and Dissertations

To determine the effects of super-dosing on pigs fed phosphorous and calcium adequate or deficient diets (Experiment 1); the optimum level of corn express phytase (CEP, Experiment 2); and the effects of xylanase/phytase on growth performance (Experiment 3) in pigs fed reduce nutrient diets, weaned pigs were blocked with initial body weight (BW) and allotted to dietary treatments. Treatments were: Positive control (PC), P/Ca adequate diets (NRC 2012); Negative Control (NC), low P (-0.15%) and Ca (-0.12%), +0, 1000, and 4000 FTU/kg of CEP in both PC and NC (Experiment 1); PC, P/Ca adequate diets; NC, decreased P (0.15%) and …

Characterization Of Bacteriorhodopsin And Halorhodopsin Reconstituted In Lipid Bilayer Membranes, Joel Domkam Kamwa

Graduate Theses and Dissertations

Motivated to produce electricity with photon activated ion pumps, the main purpose of this work was to characterize the photosynthetic membrane proteins bacteriorhodopsin (proton pump) and halorhodopsin (chloride pump). The proteins were re-suspended in lipid bilayers. For this work, an experimental set-up was built which included: chambers for lipid bilayer formation and characterization, lasers for ion pump activation, and an AxoPatch electrophysiology system for small photocurrent measurement. Lipid bilayer membranes were formed using mostly folding method: folding two monolayers together. The membranes were characterized by their resistance, capacitance, and generated photocurrent. Photocurrent was generated upon illumination of lipid-protein membranes with …

The Impact Of Upc Placement On The Biomechanical Injury Risk Factors Associated With Cashier Work, Marcus Payne

Graduate Theses and Dissertations

The purpose of this study was to investigate the impact of UPC placement on upper-body kinematics and muscle activity associated with cashier work. Seventeen female participants who had worked at least 1000 hours as a cashier and did not meet any exclusion criteria were recruited. Multi-sided UPC items, which included extra and/or larger barcodes, were compared to items with a traditional UPC placement. Two mock carts of eighteen items were scanned for each UPC type. Electromyography was applied bilaterally to the biceps brachii, middle deltoid, flexor digitorum superficialis, and upper trapezius muscles. Cumulative and peak muscle activity were calculated for …

Defending Wild Dogs: Population Dynamics And Disease In Endangered African Wild Dogs, Elizabeth Claire Arredondo

Graduate Theses and Dissertations

African wild dogs (Lycaon pictus) are endangered carnivores whose population is decreasing from habitat loss and fragmentation, interspecific competition, and disease. Survival rates are especially low in Kruger National Park (KNP), though it is unclear why. I estimated the abundance in KNP and survival rates over different time spans, six years and nine months, using public photographic survey data. In 2015, there were 298 (SE=12.1) individuals. Using a mark-recapture analysis in program R, I found that the survival rate between 2009-2015 was only 3.2%, and within the 9-month survey period (September 2014 – June 2015), monthly survival rates for the …

Animal Welfare Frames: How Social Media Messages Bridge The Gap Between The Protein Industry And Consumers, Olivia Norton

Graduate Theses and Dissertations

The two articles in this thesis used content analysis to analyze and compare animal welfare related website and Twitter content of the top five animal protein producing companies in the United States. In the first article, the animal welfare website content of Cargill, Tyson Foods Inc., Smithfield, JBS® and Sysco were analyzed for persuasive frames, common topics, and key terminology to describe their corporate positions on animal welfare. Sysco’s main page devoted to animal welfare dominated the word count with 1,045 words, while JBS®’s main animal welfare page used only 265 words to communicate the company’s views. The most commonly …

New Technologies For Evaluating Putting Green Surface Characteristics, Daniel Patrick O'Brien

Graduate Theses and Dissertations

Golf course putting greens require a high level of inputs predicated on timely, well informed decisions. Putting green quality is ultimately defined by performance of the turfgrass, and this performance encompasses both (i) the health and vitality of the turfgrass plants, and (ii) the ability of the turfgrass to exist as a playing surface, as it interacts with the golf ball. For golf course superintendents, accurately and efficiently assessing moisture levels and nutrient status are critical for guiding maintenance practices. This research sought to examine new ways for measuring each of these parameters, and compared them to ground-truth data and/or …

Shallow Aggregate Ebb-And-Flow System For Greenhouse Lettuce Production, Joseph Chidiac

Graduate Theses and Dissertations

A novel soilless technique for the production of lettuce was developed and evaluated for its viability for ornamental greenhouse growers adapting their ebb-and-flood irrigation benching systems to diversify into growing lettuce, without having to purchase the conventional nutrient film technique (NFT) or deep flow technique (DFT) hydroponic systems. The experimental design was a three by four factorial, with three treatments for aggregate depth (19, 38, and 57 mm) and four treatments for irrigation frequency (once every 1, 2, 4, or 8 hours), for a total of twelve treatment combinations. Each treatment was replicated three times in a randomized complete block …

Transcriptome-Based Gene Networks For Systems-Level Analysis Of Plant Gene Functions, Chirag Gupta

Graduate Theses and Dissertations

Present day genomic technologies are evolving at an unprecedented rate, allowing interrogation of

cellular activities with increasing breadth and depth. However, we know very little about how the

genome functions and what the identified genes do. The lack of functional annotations of genes

greatly limits the post-analytical interpretation of new high throughput genomic datasets. For plant

biologists, the problem is much severe. Less than 50% of all the identified genes in the model plant

Arabidopsis thaliana, and only about 20% of all genes in the crop model Oryza sativa have some

aspects of their functions assigned. Therefore, there is an …

In The Field The Women Saved The Crop: The Women’S Land Army Of World War Ii, Denna M. Clymer

Graduate Theses and Dissertations

The Women’s Land Army brought together rural and urban sectors of the United States in a climate of national and regional crisis. By the time the country was cast into war, the agricultural sector was already caught in a downward economic spiral that drove away laborers. With demand falling, and farms propped up only by experiments in subsidy and parity, when military and industrial jobs emerged in urban areas, farm laborers became scarce. At the same time the war created jobs for men outside of the agricultural sector, farm prices recovered and demand soared, forcing farmers to look to women …

Fall Risk In Sedentary, Recreationally Active, And Masters Athlete Older Adults, Felicia Elizabeth Squires

Graduate Theses and Dissertations

The purpose of this study was to examine whether different habitual activity levels may affect balance ability in older adults. Balance must be studied as poor balance increases likelihood of falls. This study examined activity levels in older adults and effect on balance ability. Fifty-nine older adults aged 66.5 ± 9.5 participated. Three groups were separated by physical activity level. First group had 13 masters athletes, older adults performing least 150 minutes of aerobic activity a week and competing in nationally sanctioned event within the last six months. Twenty-seven older adults comprised recreationally active group performing at least 150 minutes …

Identifying Genetic Factors Influencing Sperm Mobility Phenotype In Chicken Using Genome Wide Association Studies, Primordial Germ Cell Transplantation, And Rnaseq., Sohita Ojha

Graduate Theses and Dissertations

Sperm mobility is a major determinant of male fertility in chicken. In spite of low heritability of reproductive traits, sperm mobility has high heritability index which suggests presence of quantitative trait loci (QTLs) governing the trait. Our research focused on three objectives: i) to identify the QTLs affecting low mobility phenotype in chicken, ii) to understand the impact of Sertoli-cells and germ cells interactions in influencing the mobility phenotype and iii) to identify the genes and gene networks differentially expressed in male and female PGCs. To detect the QTLs, genome wide association studies (GWAS) was conducted which revealed the presence …