Life Sciences Commons^™

Examining The Effects Of Oral Contraceptive Use On Thermoregulation, Whitley C. Atkins

Graduate Theses and Dissertations

Purpose: The purpose of this investigation was to evaluate the effects of combined (estradiol and progestin) monophasic oral contraceptive pill (OCP) use on thermoregulation. Further, we sought to evaluate OCP use on acute rehydration post-exercise in the heat using recommended rehydration guidelines. Methods: Twelve healthy, aerobically trained (VO2peak = 47.8 ± 4.7 mL/kg/min), long term female oral contraceptive users completed a familiarization trial and two experimental days separated by ≥ 7 days. The two experimental trials were identical except for the hormone dosing phase. One trial was completed during the third week of active pill dosing (ACT) and one during …

Predictors Of Peak Elbow Valgus Torque In Collegiate Baseball Pitchers, Adam Richard Nebel

Graduate Theses and Dissertations

Context: The incidence of UCL tears in baseball is at an all-time high. ATs are in the position to identify those at risk and potentially prevent injury to the UCL. In baseball, research has associated elbow valgus torque as a potential predictor of injury risk. However, markerless analysis has not assessed possible predictors of injury to the UCL in baseball players. Objective: Identify the kinematic factors that influence peak elbow valgus torque through the sequence of a fastball pitch. Design: Cross-sectional study. Setting: Field study performed in university’s pitching development center. Participants: Division 1 collegiate baseball pitchers (N=21; 17RHP, 4LHP; …

Efficacy Of Multivitamin-Mineral Supplementation On Measures Of Anxiety, Depression, Self-Esteem, Dysregulation, And Perceived Stress In Young Adults, Courtney Gorden

Graduate Theses and Dissertations

Mental health in the United States is at an all-time low with 21% of adults 18+ suffering from depression or anxiety. When compared with other categories, emerging adults (18-25 years old) have the highest prevalence of mental health disorders at 30%. Young adults face an inordinate amount of stress given the COVID-19 pandemic, being in a unique developmental stage, experiencing new relationships, and transitioning from high school to their professional lives. Vitamins and minerals have roles in neurobiochemistry and have been investigated for effects on mental health with mixed results. Few studies factor in the unique emerging adult developmental stage …

Arkansas Fresh-Market Blackberries: Identifying Unique Attributes And Harvest Practices That Impact Marketability, Andrea Lea Myers

Graduate Theses and Dissertations

Fresh-market blackberries (Rubus subgenus Rubus) are sold worldwide and have attributes that appeal to consumers. The delicate-nature of the fruit requires hand harvesting, and minimal handling of the fruit postharvest. Objectives of this research on Arkansas fresh-market blackberries was to 1) identify the unique attributes 2) determine the best handling practices to increase postharvest quality, and 3) evaluate the potential of soft robotic gripper for harvesting. For the first objective, blackberry genotypes were harvested from the University of Arkansas System (UA System) Division of Agriculture Fruit Research Station in Clarksville, AR. Nineteen genotypes were harvested in 2020, eight genotypes were …

Effects Of Cognitive Style On Food Perception And Eating Behavior, Thadeus Lyndon Beekman

Graduate Theses and Dissertations

Within the fields of psychology, notably cultural psychology, the analytic-holistic cognitive style theory has been introduced, developed, fine-tuned, and validated across a wide range of situations, stimuli, and populations. This research, combined with recent applications of the analytic-holistic theory, suggests that the differences in analytic or holistic tendencies of individuals in food, sensory, and consumer tests can impact food perception and associated behaviors. This dissertation aimed to investigate the impact of analytic-holistic cognitive styles of consumers in food situations. The first objective to accomplish this goal was to conduct exploratory research to identify if and where the analytic-holistic theory may …

Teaching Programmable Microcontrollers To Novice Users In A College Of Agriculture: Effects On Attitude, Self-Efficacy, And Knowledge, Grant T. Hood

Graduate Theses and Dissertations

This thesis consists of two articles that examined an instructional treatment based on the use of Arduino UNO R3 programmable microcontrollers in a fundamentals of agriculture systems technology course at the University of Arkansas. The first article examined students’ breadboarding and programming self-efficacy and knowledge of Arduino. The treatment consisted of a three-class-period instructional treatment, starting with a pretest before instruction to measure students’ baseline interest, knowledge, and self-efficacy of breadboarding and programming Arduino. This was followed with a short 30-minute instructional video explaining basic Arduino programming and breadboarding. Next a hands-on laboratory activity requiring students to breadboard and program …

Managing Stress Tolerance On Warm-Season Putting Greens In The Transition Zone, Thomas Edward Walton

Graduate Theses and Dissertations

The use of warm-season putting greens in the transition zone has increased in recent years. Ultradwarf bermudagrass (Cynodon dactylon (L.) Pers x C. transvaalensis Burtt-Davy) is the most prevalent warm-season putting green selection in the transition zone, however, newly developed greens-type zoysiagrass (Zoysia spp.) cultivars represent another potential selection for golf courses. The two major limitations of warm-season grasses in the transition zone are a general lack of cold- and shade-tolerance. Protective covers are essential to protect ultradwarf bermudagrass putting greens in the winter months. Unfortunately, golf courses can still experience winterkill underneath protective covers. Two field trials were conducted …

The Role Of Ccaat Binding Factor In The Regulation Of Catalase Gene Expression In Candida Albicans, Zahra Al-Rumaih

Graduate Theses and Dissertations

Candida albicans is a fungal opportunistic human pathogen. Its infections range from surficial infections like skin rash to fatal systemic infections. Filamentation growth mode is associated with C. albicans virulence because it helps penetration of the host’s epithelial cells. The CCAAT-binding factor (CBF) is a conserved heterooligomeric transcription factor found in 30% of eukaryotes genes. In C. albicans it is composed of 4 major subunits, including Hap2, Hap3, Hap4, and Hap5. Hap2 and Hap5 are essential for DNA binding and function. Hap4 has 3 homologous subunits: Hap41 and Hap42 are putative subunits of CBP. Hap43 is the only Hap4 subunit …

Bumpers College Of Agricultural, Food And Life Sciences Professional Development Opportunities And Their Impact On Perceptions Of Career Preparedness, Jessica L. Wesson

Graduate Theses and Dissertations

A student’s undergraduate education is an imperative part of developing career skills that will prepare them to transition from academia to the professional world. Experience outside of the classroom and course material taught in a formal class experience are both important. Their experiences and classes, in college, allow them to develop skills and increase their chances of being successful in the workplace (Suvedi et al., 2016).

Chickering’s Seven Vectors of Student Development were used to help explain the process of student development. A quantitative survey was sent to DBCALFS faculty, staff, and administrators, and a different quantitative survey was sent …

Multi-Trophic Biodiversity Increases With Increasing Structural Complexity Of Forest Canopy, Ayanna St. Rose

Graduate Theses and Dissertations

Understanding the effects of forest canopy structural complexity on multi-trophic diversity is critical for conserving biodiversity and managing land sustainably. But multi-trophic diversity is often ignored when making decisions about land management due to lack of cost- and time-effective methods to evaluate it. Here, we explored a new method based on widely available remote sensing data to quantify canopy structural complexity and its relationships with multi-trophic biodiversity at landscape scale using 32 forested sites of the National Ecological Observatory Network. We investigated the influence of vertical and horizontal structural complexity of forest canopy on multi-trophic (primary producers, herbivores (beetles), omnivores …

Preparation Of Proteins And Peptides From Heat-Stabilized Defatted Rice Bran Via Solid State Fermentation And Investigating For Skin Health, Ali A. Bisly

Graduate Theses and Dissertations

Heat-stabilized defatted rice bran (HDRB) is an abundant and inexpensive agro-industrial by-product of rice milling and rice oil extraction process. Furthermore, HDRB is a potential source of proteins. However, the direct protein extraction has low yield of protein due to the impact of heat stabilization and oil extraction process which made the HDRB proteins bound and interact with other components. This research is based on using solid-state fermentation (SSF), which is a natural and cost-effective approach to facilitate protein extraction from HDRB using Bacillus subtilis (natto) Takahashi as a microorganism.

Response surface methodology (RSM) analysis was used with Box-Behnken design …

Genetic Control Of Prickles And Plant Height In Blackberry, Carmen Johns

Graduate Theses and Dissertations

The fresh-market blackberry (Rubus L. subgenus Rubus Watson) industry has been expanding for the past two decades. Blackberry market growth can be attributed to many factors including expanded production regions, improved production systems, and release of superior cultivars. Blackberry breeding is a time- and labor-intensive process which would benefit from implementation of molecular markers for highly heritable, important traits. Next generation sequencing, a high-quality reference genome, and software capable of analyzing this complex genome were applied in a genome-wide association study (GWAS) to reveal maker-trait associations related to prickles and plant height variation in fresh-market blackberry. In 2020 and 2021, …

Macronutrient Availability Shapes Host Response To Infection And Feeding Behavior, Weston Gerald Perrine

Graduate Theses and Dissertations

Macronutrients in the diet are vital to the physiological processes necessary for an organism to effectively clear a pathogen. Diet can be important to a host’s susceptibility to infection and severity of pathology, though results can vary across host-pathogen systems (Sen et al. 2016). Manipulating the ratio of specific macronutrients in the diet is an effective method to begin understanding how individual macronutrients, rather than food types, have on immune responses. Using an avian host-pathogen system, I explored the effects of dietary macronutrient composition, specifically lipid and protein content, on disease pathology and behavior of canaries (Serinus canaria) infected with …

Teaching For Career Success: An Agricultural Industry Perspective Of Preparedness Needs For Diverse Workforce Development, Madelaine Giebler

Graduate Theses and Dissertations

This research examined agricultural graduate preparedness needs through the lens of agricultural industry professionals. In the rapidly changing industry environment, continual review of agricultural curriculum needs provides relevant curriculum recommendations for academic programs. This study came at a time when agricultural curriculum was in need of review. Methodology for this study involved a qualitative mixed methods approach employing survey and interview responses. A total of 121 industry professionals, identified through the University of Arkansas Bumpers College Career Development Center, were contacted with the survey link. A total of eighteen individuals completed the surveys, and of those eighteen survey respondents, eight …

Variations In Consumer Rejection Thresholds Of Water Samples Including Mixed-Berries Flavors, Kathryn Mccullough

Graduate Theses and Dissertations

Functional flavored water has emerged as a major space in the beverage industry in recent years. However, no research has explored the point that consumers begin to reject a specific flavor concentration within a flavored water matrix (i.e., a consumer rejection threshold). The first part of this thesis aimed to determine the consumer rejection thresholds of mixed-berry flavors in both sweetened and unsweetened water samples and examine the effects of demographics, food neophobia status, and personality traits on the consumer rejection thresholds. The second part of this thesis aimed to distinguish and compare consumer rejection threshold methodologies with and without …

Rural Students On College Enrollment: Perceptions Of Influence Surrounding College Choice, Lucas M. Simmons

Graduate Theses and Dissertations

The purpose for conducting the study was to describe the community, personal experiences, and life events of individuals from rural Arkansas communities in developing their decisions to attend college and how the community impacted their successful enrollment at the University of Arkansas. Specifically, the study set out to explore how rural communities were perceived to influence college enrollment decisions and choices in degree selection. Although situated at one case study institution, the University of Arkansas, the findings have importance for all rural students and communities, and ultimately public policy and institutional behaviors. Therefore, the purpose for conducting the study was …

Characterization Of The Influence Of A Small Molecule Inhibitor On Ras-Related Proteins Interactions, Emilio Duverna

Graduate Theses and Dissertations

The Ras superfamily of small G proteins are involved in cell-signaling processes that, if not regulated, may lead to cell multiplication, apoptosis inhibition, and tumorigenesis. They function as molecular switches, which through GTP/GDP exchange cycle, switch on or off cellular activities. Overexpression and/or hyperactivity of these proteins have been linked to many diseases including various cancers. CDC42, a member of the Rho subfamily of the Ras superfamily of small G proteins, participates in the regulation of many cellular processes including cell adhesion, mitosis, and cytoskeletal rearrangements. CDC42 binds to and activates many effector proteins including CDC42-activated kinase (ACK). Abnormal activities …

Investigating Molecular Mechanisms Behind Bacterial Chondronecrosis With Osteomyelitis (Bco) Pathogenesis In Modern Broilers, Alison Ramser

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis (BCO), a leading cause of lameness in broiler chickens, is characterized by infection, inflammation, and bone attrition. There are currently no effective treatments and positive diagnosis is only possible through necropsy evaluations. Lameness is also a rising animal welfare and economic concern, making prevention and detection of BCO all the more critical. These challenges are exacerbated by a lack of mechanistic understanding of BCO’s etiology. The question I asked during my dissertation was how bacteria induce bone attrition in BCO pathology. My research has shown that mitochondrial dysfunction is characteristic of BCO conditions along with autophagy …

Reconstructing Bison And Mammoth Migration During The Late Pleistocene And Early Holocene Of Central Texas Using Strontium Isotopes, Joshua John Porter

Graduate Theses and Dissertations

During the Late Pleistocene (LP; past 130,000 years), over two-thirds of large mammal (>45kg) species went extinct globally. While the role of humans is hotly debated, the effect of these extinctions is growing clearer; the extinctions resulted in widespread and lasting faunal community reorganization. However, the impact of these extinctions on dietary and migratory behavior within faunal communities is unknown. Our study examines the impact of the megafaunal extinctions on the dietary and migratory behavior of surviving Bison individuals in Texas using carbon, oxygen, and strontium isotopes. Strontium isotopes are incorporated into mammalian enamel during their tooth development and …

Evaluation, Characterization, And Utilization Of Weed-Suppressive Sweetpotato Cultivars For Sustainable Weed Management, Isabel Schlegel Werle

Graduate Theses and Dissertations

Sweetpotato (Ipomoea batatas L.) is a low-technology, subsistence crop that enhances food and nutrition security particularly in developing nations. Weed interference with the crop can reduce sweetpotato storage root yields and product quality. Current weed management practices in sweetpotato include PRE or POST herbicides application, cultivation, mowing, or handweeding. Unlike row crops, herbicide options for sweetpotato are few; therefore, alternative weed control practices are needed. The overall objective of this research was to determine the weed suppressive ability of several sweetpotato cultivars. This research also provides information about cover crop use for weed suppression in sweetpotato production in Arkansas. Field …

Implementing An Exercise Referral Scheme For Promoting Physical Activity In University Mental Health Care, Bryce Daniels

Graduate Theses and Dissertations

Introduction. Mental health symptoms and conditions are prevalent and increasing on college campuses with anxiety and depression having the highest increases in prevalence. Many college students are also physically inactive which is problematic as physical activity benefits mental health. Exercise referral schemes (ERS) can promote physical activity, though no studies have investigated the implementation of an ERS within a college counseling center. Purpose. The purpose of this dissertation was to investigate the implementation of an ERS for promoting physical activity in university mental health care. Methods. Study 1 used a qualitative descriptive design of 14 certified counselors to explore perceptions …

Impact Of Genetic Variation And Timescale On Diatom Salinity Stress Response, Kala M. Downey

Graduate Theses and Dissertations

Natural environments are dynamic, and organisms must sense and respond to changing conditions. One common way organisms deal with stressful environments is through gene expression changes, allowing for stress acclimation and resistance which occurs over varying time spans in different species. The recent evolutionary history of populations could greatly influence their ability to respond successfully. An evolutionary history in disturbed or fluctuating conditions could promote increased resistance or a more rapid response to these environmental stressors. To understand the impact of genotypic variation and timescales on response and acclimation to salinity changes, we have been exploiting the abilities of euryhaline …

Evaluating The Effects Of Cardiorespiratory Fitness Level And Gender On Outcomes Of The Buffalo Concussion Bike Test, Katie Stephenson

Graduate Theses and Dissertations

Background: Accurate assessment of concussion is crucial for creating a treatment plan. The Buffalo Concussion Bike Test (BCBT) is a 30-minute graded exercise protocol for a stationary bicycle. Previously this assessment has been reportedly used to screen for exercise intolerance secondary to autonomic dysfunction; however, the original purpose of the assessment was to find a sub-symptom threshold for exercise rehabilitation during concussion recovery. Starting resistance for the BCBT protocol is based solely on body mass. Other factors such as cardiorespiratory fitness level and sex have been noted to effect outcomes on similar assessments and are not considered in the BCBT …

Diffusive Dynamics Of Biological Macromolecules In The Cytoplasm Of Live Bacteria, Asmaa Ali Sadoon

Graduate Theses and Dissertations

Bacteria are small organisms that play important roles in our bodies and in maintaining our surrounding environment. However, bacterial infections are considered as one of the health diseases that threatens the lives of humans. Resistance of bacteria to antibiotics has made treating bacterial infections more challenging. With the emergence of many developed optical techniques, it has become possible to study various biological mechanisms of molecules inside these small organisms with high resolution and precise localization. In this research, single particle tracking photoactivation localization microscopy (sptPALM) technique was used to investigate the dynamics of fluorescent histonelike nucleoid-structuring proteins (H-NS) in live …

Evaluation Of Benzobicyclon In Midsouth Rice (Oryza Sativa) Production Systems, Jake Patterson

Graduate Theses and Dissertations

Controlling weedy rice postemergence is challenging for rice producers in the United States because of the lack of herbicide options. Weedy rice is genetically similar to cultivated rice, thus making it difficult to control with mid-season postemergence herbicide applications without also damaging the crop. Hence, there is a need for a new effective postemergence weedy rice control herbicide. Findings from this research indicate that the use of benzobicyclon in current standard quizalofop- and imidazolinone-resistant rice herbicide programs provides tremendous utility for Midsouth rice producers. In both of these production systems, the addition of benzobicyclon to the respective standard herbicide programs …

Evaluation Of Fenclorim Safener For Use In Rice With Group 15 Herbicides, Tristen Heath Avent

Graduate Theses and Dissertations

The development of herbicide resistance and the lack of effective herbicides to control problematic weeds has caused Arkansas rice (Oryza sativa L.) production to pursue alternative sites of action. Currently, very long-chain fatty acid elongase inhibitors are not labeled for U.S. rice production but have been widely used for Asian rice production systems. Previous research has demonstrated the utility of acetochlor and pyroxasulfone to provide in-season weed control for Arkansas rice production, but variable crop tolerance has been observed. Additionally, acetochlor at 1,260 g ai ha-1 elicited less rice injury when seeds were treated with a herbicide safener seed treatment …

To The Window, To The Wall: Improving Environmental Monitoring Protocols, Sarah Jones

Graduate Theses and Dissertations

Detecting microorganisms on environmental surfaces via an environmental monitoring (EM) program is part of a preventive food safety culture. Environmental monitoring should 1) verify that food safety plans are reducing cross-contamination risk from surfaces to food, 2) pinpoint microbial niches, and 3) prevent the transmission of pathogens. Environmental monitoring programs utilize EM tools, such as sponges, to sample food contact and non-food contact surfaces. However, EM tool selection is determined by the individual food firm. This dissertation evaluated and characterized factors influencing EM program effectiveness in the food industry. Specifically, this dissertation focuses on the release of microorganisms from EM …

Development Of A Long-Read Sequencing Protocol To Assess The Precision And Efficacy Of Gene Editing For Duchenne Muscular Dystrophy, Landon Andrew Burcham

Graduate Theses and Dissertations

This work establishes a method for assessing on-target precision due to CRISPR-Cas9 gene editing, especially within the context of exon skipping therapy for Duchenne Muscular Dystrophy. The proposed method utilizes an Oxford nanopore long-read sequencing approach to sequence amplified regions of DNA that have been edited using CRISPR-Cas9. NIH3T3 and C2C12 cell lines were treated with a dual-guide CRISPR-Cas9 system, that targets and deletes exon 23 from the DMD gene in mouse samples. Deletion PCR revealed deletion of exon 23 in both DNA and cDNA samples. Additionally, sequencing using Oxford Nanopore revealed targeted exon 23 deletion as the most prevalent …

Using Thermal Units To Predict Biomass Accumulation And Total Nitrogen Uptake For Cover Crops In Arkansas, Mila Victório Pessotto

Graduate Theses and Dissertations

Including cover crops in agricultural production systems is an important management practice. Cover cropping can improve soil health, increase plant-available nitrogen (N), provide surface residue to prevent erosional soil loss, increase water infiltration, and increase weed suppression. Cover crops growth can be predicted using thermal days or growing degree days [GDD] similar to commodity crops such as corn (Zea mays L.) or rice (Oryza sativa). Growing degree day calculations are a well-known tool to predict crop growth stage or development stage and can be adapted for use in any plant species, including cover crops. Identifying and developing the relationship between …

The Impact Of Dietary Protein Supplementation As Part Of A Time Restricted Feeding Eating Pattern On Sleep, Mood, And Body Composition In Adults With Overweight Or Obesity, Rebecca L. Bowie

Graduate Theses and Dissertations

Obesity in the United States continues to increase, and effective, long-term weight loss strategies are limited. Time restricted feeding (TRF) is a dietary intervention that has potential to serve as an effective long-term weight loss strategy, however more data is needed to establish the effectiveness of a TRF eating pattern on obesity and its related health outcomes. To our knowledge, manipulation of macronutrient composition during TRF intervention and the effect of TRF on sleep and mood have not been studied. Therefore, the objective of this study was to determine the effect of protein supplementation during TRF on mood, sleep, and …