Life Sciences Commons^™

Local And Systemic Inflammatory And Antibody Responses To Salmonella-Killed Vaccines In Egg-Type Pullets, Jossie Mair Santamaria Archbold

Graduate Theses and Dissertations

Salmonella infection in chickens is a significant and complex challenge to manage, affecting poultry health and food safety. Vaccination against Salmonella is a promising preventative approach to control flock infection and decrease the loads of this pathogen. Vaccination strategies against Salmonella often comprise the application of live attenuated and killed (inactivated) vaccines, including inactivated autogenous vaccines. Live attenuated Salmonella vaccines stimulate both cellular and humoral immunity but can potentially return to a virulent state; meanwhile, inactivated Salmonella vaccines trigger more of a humoral response and are safer since they are entirely rendered inactive. However, there is a lack of information …

Perceptions Of School-Based Agricultural Education Teachers Toward Promoting Agricultural Education Teaching As A Career, Emmanuel Kwadwo Sekyere

Graduate Theses and Dissertations

School-based agricultural education has experienced a scarcity of qualified teachers over the years. To reduce the prevalence of school-based agricultural teacher shortages, research has led to the adoption of several approaches in schools across the country. The purpose of this study was to examine the perceptions of school-based agricultural education teachers toward promoting agricultural teaching as a career. The theoretical framework for this research was based on the Theory of Planned Behavior. The research objectives were to determine the perceived importance of career promotion activities, the frequency of career promotion activities, the perceived job satisfaction of SBAE teachers, and the …

Turfgrass Soil Surfactants: Examining Active Ingredients, Application Strategies, And The Structure–Function Relationship In Sand-Based Putting Greens, Daniel O'Brien

Graduate Theses and Dissertations

Soil surfactants, commonly referred to as wetting agents, are a leading water management strategy for golf courses. Non-ionic block copolymers are the most common type of turfgrass soil surfactant. However, differences in the registration process and labeling requirements allow soil surfactants to enter the marketplace accompanied by far-less data compared to other products used on golf courses. This has led to considerable confusion among golf course superintendents. Three primary sources of confusion are: (i) the widely-used marketing terminology of “penetrants” and “retainers” to classify wetting agents; (ii) active ingredients which in some cases are not disclosed at all; and (iii) …

Bilateral Asymmetry In The First Metacarpal: The Influence Of Handedness In Humans And Non-Human Primates, Lucyna A. Bowland

Graduate Theses and Dissertations

Muscle attachment sites (entheses) are often used to infer soft tissue anatomy and reconstruct behaviors within skeletal populations, but there remains a significant debate about whether and to what degree muscle use is accurately reflected in bony morphology. One of the most difficult problems in establishing the relationship between variation in muscle use and entheseal development is the inability to exclude a variety of external factors thought to influence enthesis development, including age, sex, body mass, and activity patterns. One way around this is to take advantage of the fact that humans are handed (preferentially using one hand over another, …

Exploring The Effects Of N,N-Dimethylbiguanide And Ω-3 Pufas On Muscle Stem Cell Differentiation, Enayah Alhshim

Graduate Theses and Dissertations

The impacts of omega-3 (ω-3) fatty acid and metformin hydrochloride (Met-HCl) on muscle stem cells differentiation, mimicking the fetal muscle development under gestational diabetes mellitus (GDM), were investigated. ω-3 fatty acids have been known for their ability to regulate adipogenic transcription factors such as Sterol Regulatory Element-Binding Protien (SREBPs) and Peroxisome Proliferator-Activated Receptors (PPARs). This regulation resulted in gene expression modification for lipid metabolism and adipocyte differentiation, thus preventing excessive fat accumulation. Additionally, emerging evidence suggested that ω-3 fatty acids may also exert direct effects on myogenic processes that influence muscle stem cells proliferation, differentiation, and maturation. Meanwhile, Met-HCl, an …

Identifying Grassland Cores In The Southeast: Relating Spatial Covariance To Avian Communities And Vegetation Structure, Lauren L. Berry

Graduate Theses and Dissertations

Globally, grasslands are declining in range, which is leading to a decline in grassland-dependent taxa. These declines can be attributed to a variety of issues, a major one being the encroachment of woody species into historic grasslands. There is a new framework for strategizing grassland management, referred to as “Defend the Core,” which calls for proactive management of intact grasslands, meaning conserving remaining grasslands by preventing woody encroachment in the first place. To successfully defend the core, it is imperative that we develop rapid methods to identify intact grassland cores for management priority. One method that can be used for …

Genome-Wide Association Study/ Genomic Prediction Of Cowpea Seed Protein And Black Seed Coat Color In Cowpea, Yilin Chen

Graduate Theses and Dissertations

Cowpea (Vigna unguiculata [L.] Walp) is a crucial crop in many regions, serving as a vital protein source for humans and livestock. Addressing the need to enhance its nutritional quality and make it easier to cultivate cowpeas of specific colors, this study utilized genome-wide association studies (GWAS) to identify markers associated with protein content and seed coat color patterns in cowpea.

In the protein content study, a GWAS was conducted on 161 cowpea accessions using 110,155 high-quality single-nucleotide polymorphisms (SNPs). Seven significant SNP markers were identified, all located at a locus on chromosome 8 associated with the gene Vigun08g039200, enhancing …

Evaluation Of Late Season Potassium Applications To Arkansas Soybean, Carrie Ortel

Graduate Theses and Dissertations

Potassium (K) deficiency is one of the most important yield limiting factors in Arkansas soybean (Glycine max (L.) Merr.) production, although there are recently improved diagnostic and management opportunities available. However, several questions regarding the successful implementation of in-season K management in soybean production remain. Therefore, the research objectives were to (a) delineate the relationship between drought stress and K deficiency on soybean vigor, (b) identify the spatial dependencies of leaf-K concentrations at the production scale, (c) develop a leaf sampling protocol, (d) calibrate the fertilizer-K rate needed to correct varying levels of K deficiency at 15, 30, and …

Vegetation Management During Establishment Of Native Grasses And Forb Plantings On Roadsides And Prairie Restoration, Rachel Woody-Pumford

Graduate Theses and Dissertations

North American prairies have been shrinking due to agricultural production, overgrazing, development of cities and towns, and climate change. However, integrated roadside vegetation management (IRVM) creates the opportunity to reestablish prairies along roadsides through planting native grasses and forbs. Utilizing postememergence (POST) herbicides registered for use along rights-of-way has the potential to remove invasive species and create space for newly establishing native grass and forb species. The use of clopyralid, florpyrauxifen-benzyl, metsulfuron, and quinclorac were found to be safe for use in plantings containing big bluestem (Andropogon gerardii Vitman) and butterfly milkweed (Asclepias tuberosa L.). However, florpyrauxifen-benzyl injured and stunted …

The Effect Of Microbial Bile Acid Metabolism On Clostridium Perfringens Virulence, Tahrir Jatli Alenezi

Graduate Theses and Dissertations

Clostridium perfringens is a predominant intestinal pathogen affecting both humans and animals, including chickens resulting in significant economic losses. In chapter I, I have overviewed the dynamics of the intestinal immune system, the composition of the intestinal microbiome, and how the microbiome influences inflammation in the development of Clostridium infection. The intricate interplay between immunity, microbiota, and their metabolites is likely critical in the pathogenesis of C. perfringens and uncovering these interactions may unveil new avenues for prevention and treatment. The objective of this chapter is to provide updated insights into the role of host-microbe interactions and their potential therapeutic …

Barriers To Participation In Research Sae Projects And Ffa Agriscience Fair Competitions, Jessica Hall

Graduate Theses and Dissertations

Agricultural education plays a pivotal role in preparing students for careers in the ever-evolving field of agriculture. This study investigates the professional development and training needs of school-based agricultural education (SBAE) teachers in the context of research SAE projects and participation in FFA agriscience fair competitions. Through a comprehensive literature review and empirical inquiry, this study aims to identify teachers' confidence levels, perceived importance of tasks, professional development needs and barriers hindering student and teacher involvement in these activities. The research objectives were structured to address key aspects of SBAE teacher preparedness and student engagement. Findings reveal areas where teachers …

Dispersal Movement And Overwintering Ecology Of A Cryptic Migratory Forest Owl, Mitchell Lee Pruitt

Graduate Theses and Dissertations

Understanding species’ distributions and how a species uses its environment is a vital aspect of ecology and important for the development and implementation of conservation planning. In migratory species, with distinct breeding and nonbreeding ranges, understanding distributions is a complex endeavor. Nevertheless, evaluating movement behavior by linking seasonal distributions and identifying source areas is vital for understanding a species’ full annual cycle ecology. Understanding distributions is particularly important for predators, like birds of prey, that can exert top-down effects on community structure and ecosystem function through their influence on prey populations. Among raptors, owls are severely understudied in large part …

Assessing Consumer Willingness To Pay For Zinc-Biofortified Rice – The Case Of Colombia, Sara Ann Oswalt

Graduate Theses and Dissertations

Hidden hunger, a form of undernourishment caused by micronutrient deficiencies, remains a critical global challenge affecting billions worldwide. Biofortification of staple crops, such as rice, offers a promising solution to address this problem. However, changes in the physical and culinary characteristics due to biofortification may hinder consumer acceptance and limit the benefits of this approach. This research explores consumer preferences and willingness to pay (WTP) for zinc-biofortified rice in Cartagena, Colombia. A non-hypothetical experimental auction, including 400 consumers in five different locations within Cartagena, was conducted to analyze the impact of information, socioeconomic factors, and consumer nutrition knowledge on WTP …

Production And Heat Unit Accumulation Tracking Of Long-Cane Blackberry In Controlled Environments, Leala Machesney

Graduate Theses and Dissertations

Consumer demand for locally produced and high-quality blackberries is projected to increase. Most blackberry production is field-based, however, there is interest in producing blackberries using soilless culture and “long-cane” technique, where plants are grown in containers and trellised vertically. Soilless “long-cane” production of blackberries is viewed as a possible solution to the yield losses caused by soilborne pathogens such as Fusarium oxysporum, and methods of soil-borne pathogen control have become increasingly difficult with the ban of methyl bromide, a highly effective and widely used but environmentally harmful soil fumigant. Other reported benefits of soilless caneberry production include greater yields, increased …

Migration And Breeding Ecology Of King Rails (Rallus Elegans) In Southeast Arkansas, Jessica Schmit

Graduate Theses and Dissertations

King Rails (Rallus elegans) are a secretive marsh bird species of conservation concern in Arkansas. Freddie Black Choctaw West WMA in southeast Arkansas has multiple confirmed pairs of breeding King Rails and offered an opportunity to investigate King Rail migration ecology, as well as nesting ecology and nest site selection. In Chapter 1, we determined migratory status (resident vs. migratory) and described seasonal movement. To do this, we captured and outfitted 23 birds with Argos GPS tags to track migration patterns throughout the season. We found that most of the population did not make long distance migrations but instead were …

The Expression Of Recombinant Cas9 Proteins In E. Coli Bl21(De3) Strains, Morgan Reese

Graduate Theses and Dissertations

Abstract Extensively used for genome editing, CRISPR-Cas9 is a relatively new tool that has made a major impact in the genomic engineering landscape. Due to this, the production of CRISPR proteins that are not commercially available can limit research applications. Here, we present methods for optimizing laboratory scale production of CRISPR proteins including Cas9. The method uses IPTG to induce gene expression of the specified Cas9 and nickel column purification to isolate proteins. CRISPR proteins are validated for purity by SDS-PAGE gel, western blot, and for activity by in vitro digestion. We thoroughly validate and compare the effectiveness of BL21(DE3) …

Exploring The Potential Of Electromagnetic-Driven Energy Technologies In Preventing Microbial And Aflatoxin Contamination Of Grains, Abass A. Oduola

Graduate Theses and Dissertations

Electromagnetic-driven energy such as infrared (IR) technology holds great potential in deactivating Aspergillus flavus spores and prevents aflatoxin B1 (AFB1) contamination. Aflatoxins, highly toxic secondary metabolites primarily produced by Aspergillus species within the Flavi section (A. flavus, A. parasiticus, and A. nomius), pose substantial health risks to both humans and animals due to their significant toxicity and carcinogenic properties. Detecting and quantifying the presence and proliferation of these harmful fungi on food materials can be achieved by measuring the concentration of ergosterol. Ergosterol is a sterol compound produced in the cell membranes of fungi during their growth, providing …

Genomic Characterization Of Enterococcus Spp Infecting Chicken Embryo, Aishat Olaoluwa Lawal

Graduate Theses and Dissertations

Chicken embryo mortality has been attributed to bacterial infections and can lead to a decline in hatchability, posing potential losses in the broiler industry. This study aims to investigate the vertical transmission of specific bacterial pathogens from breeder hens to subsequent broiler flocks through MGEs and their association with host specificity. In our sampling of 360 eggs from a broiler flock, we isolated various Enterococcus species, including E. gallinarum and E. faecalis, from incubated egg yolks or aborted embryos. E. avium was found in unincubated bad yolks. Other species like Streptococcus, Lactobacillus, and Bacillus were observed but presumed to be …

The Effect Of Gentle Yoga On Average Daily Step Count, Francesca D'Agrosa

Graduate Theses and Dissertations

While walking is a widely accessible form of exercise that is highly effective at increasing overall wellness (Kadir et al., 2019; Zhao et al., 2015) and is a recommended form of physical activity for overweight and obese individuals, studies still indicate low step counts and levels of physical activity in people with obesity (Shultz et al., 2011). Sedentary overweight individuals may avoid walking for several reasons, including physiological and/or biomechanical factors that make walking more difficult. This study attempted to explore and address these avoidant behaviors by employing a single-case ABAB reversal design to implement bi-weekly gentle yoga sessions over …

Describing Benefits, Challenges, And Barriers Of Offering Adolescent Programs At Public Gardens In The United States, Hiliary Rodgers

Graduate Theses and Dissertations

Research shows a lack of educational programs for the adolescent audience at public gardens in the United States. Along with benefits that adolescent programs create for the institutions that offer them, there are also challenges associated with designing programs for this specific audience, and barriers preventing public gardens from offering them. There is little research related to adolescent programs at public gardens in the United States, aside from a 2010 study by Purcell et al. that explored the benefits, challenges, and barriers of adolescent programs. The current study aimed to determine the prevalence of adolescent programs at public gardens in …

Cooperating Teacher Mentorship Behaviors And Job Satisfaction As Predictors Of Agricultural Education Interns’ Intent To Teach, Sarah Frances James

Graduate Theses and Dissertations

Almost a quarter of agricultural education graduates do not teach SBAE upon graduating (Foster et al., 2024), however, this attrition has not been widely studied and reasons for this decision have not been identified. To increase the number of qualified teachers entering the classroom, the reasons for this attrition must be identified and addressed by universities. This study was conducted to investigate possible relationships between cooperating teacher mentorship behaviors, job satisfaction, and intent to teach in spring 2024 agricultural education interns. A survey, which combined the student teacher view part of Nesbitt and Barry’s (2023) cooperating teacher best practices instrument …

Evaluating The Effects Of 6-Day And 7-Day Fixed-Time Artificial Insemination Protocols Using Sex-Sorted Semen On Pregnancy In Beef Cattle, Nicole Noel Noga

Graduate Theses and Dissertations

In this study, 527 beef heifers from three locations across two states were enrolled between November 2020 and May 2021 for experiment 1, divided into three sub-experiments: 1A (n = 85), 1B (n = 383), and 1C (n = 59). These heifers were randomly assigned to one of four treatment groups. Treatments 1 and 2 involved a 6-day CO-Synch + CIDR protocol, including administration of gonadotropin-releasing hormone (GnRH; 100 μg IM; Factrel; Zoetis Animal Health, Parssipany, NJ) and a CIDR insert (EAZI-BREED CIDR; 1.38g P₄; Zoetis Animal Health) on Day 0, followed by prostaglandin F2α (PGF_2α; …

Gym Sexual Harassment And Bystander Intervention: An Application Of The Reasoned Action Approach, Eric Schisler

Graduate Theses and Dissertations

Objective. The purpose of this study was to modify and validate an instrument to examine factors associated with being an active bystander when witnessing sexual harassment at a gym or fitness center. Using Fishbein and Ajzen’s Reasoned Action Approach (RAA), this study aimed to better understand the influence of a person’s attitude, social norms, and perceived behavioral control (PBC) on their intention to be an active bystander when witness to someone being sexually harassed at the gym in the next 30 days. Methodology. This study utilized an exploratory sequential mixed methods design, with phase one taking place between June and …

Effects Of Varied Fertilization, Tillage, And Supplementation Strategies On Grazing Steers And Wheat Pasture Performance, Cody Everett Gruber

Graduate Theses and Dissertations

The objectives for the two experiments were to determine the impact of nitrogen (N) fertilizer rate, establishment method through no-till (NT) or clean-till (CT), and supplementation on stocker, wheat stand, and economic performance. Within study 1, 24, 1.62-ha pastures, were used over a two-year period. Pasture treatments consisted of a combination of N fertilization rate (100.88, 134.5, or 168.12 kg/ha), applied as broadcast urea and split between two equal applications of 50% at planting and the remaining 50% in the spring when daytime average temperatures trend above 10°C, and of NT establishment or CT establishment. Steers were weighed on arrival, …

Advancing Qualitative Inquiry In Agricultural Communications: An Analysis Of Theories, Methodologies, And Standards For Quality, Karli Yarber

Graduate Theses and Dissertations

This dissertation offers a deep dive into the evolving landscape of qualitative research within the field of agricultural communications. Through a trio of research articles, this research critically assesses the application of theories, methodologies, and standards for quality across studies published in six disciplinary journals from 2003 to 2023. The first manuscript explores the theoretical frameworks guiding qualitative research in agricultural communications, uncovering a diverse but scattered theoretical underpinning with Framing, Diffusion of Innovations, and Uses and Gratifications Theory emerging as the most prevalent. The second manuscript meticulously examines the methodologies employed, revealing a growing acceptance and application of qualitative …

Evaluation Of Protein Turnover, Lipid Turnover, And Calcium Utilization On Broiler Breeder Performance, Cole Umberson

Graduate Theses and Dissertations

Broiler breeder performance is based upon egg production. Breeders are reared from day of hatch to ovulation with the main goal of controlling body weight. Maintaining body weight in breeders is highly emphasized prior to first egg as to reach optimum peak production feeding increases dramatically to meet energy needs. Energy is supplied to the breeder in three forms carbohydrates (fast energy), protein (medium energy), and fat (slow energy). Carbohydrates are quickly used by the breeders, while protein is constantly being utilized by the breeder for growth and egg production, fat is stored and used lastly as the energy requirements …

Evaluating The Effects Of A Noninvasive, Commercial-Grade Neurofeedback Technology And Mindfulness On Sleep Quality In Elite Athletes, Sydney Anne Hammit

Graduate Theses and Dissertations

Abstract Proper sleep is a vital element in the lives of elite athletes due to its effects on recovery, performance, and mental and emotional health. However, despite the importance of sleep quality, elite athletes are experiencing notable difficulties with both sleep quantity and quality. While sleep is necessary for all individuals, it is specifically essential for elite athletes. Sleep quality issues can be accompanied by both physical and psychological struggles, which is concerning for the population of elite athletes who are struggling with sleep quality at higher rates than the general population. Despite the increasing awareness of sleep quality struggles …

In-Service Needs Of Selected Arkansas Agriculture Teachers Related To Precision Agriculture Systems, Henry Ofosu Akwah

Graduate Theses and Dissertations

Precision Agriculture (PA) entails the use of computer technology, including software and hardware, together with good agronomic practices to effectively utilize resources available to growers. PA may enhance profitability, sustainability, resilience, and efficiency when implemented successfully. The aim of this study was to identify, assess, and describe the PA in-service needs of selected high school agriculture teachers in Arkansas. Forty-four teachers participated in the study, recruited through convenience sampling during the annual Arkansas agriculture in-service workshop held at Camp Couchdale in July 2023. A survey instrument was developed, and all forty-four participants provided complete responses. The survey included twenty-nine PA …

Determination Of Factors Influencing Microbial Food Safety Risks Of Additive Manufacturing And 3d Printing Of Food, Allyson Nichole Hamilton

Graduate Theses and Dissertations

The burgeoning field of 3D food printing introduces opportunities for customizing the physicochemical properties of food, offering tailored solutions in color, shape, and texture. Despite its potential, the implications of this technology on food safety, particularly the efficacy of manufacturer-recommended cleaning protocols for stainless steel food ink capsules, the transferability of pathogens from a contaminated food ink capsule into the food ink based on macronutrient composition of the food, and the impact of storage conditions on pathogen viability, have not been thoroughly investigated. This dissertation provides a comprehensive analysis of these aspects, focusing on the control of foodborne pathogens and …

Gaseous Ozone To Improve The Microbial Safety Of Spices, Seeds, And Nuts, Arshpreet Kaur Khattra

Graduate Theses and Dissertations

Due to the increasing number of reported outbreaks of pathogens in low-moisture foods (LMFs), there is a need to investigate novel non-thermal gaseous technologies to increase the microbiological safety of spices, nuts, and seeds without compromising the product quality. The objective of this study was to inactivate Salmonella spp. in different spices, seeds, and nuts using gaseous ozone treatment and evaluate the suitability of Enterococcus faecium as a potential surrogate for Salmonella. Each food product (dried basil leaves, black peppercorn, chia seeds, and walnuts) was separately inoculated with a 5-strain Salmonella and E. faecium cocktail and equilibrated to water activity …