Life Sciences Commons^™

Lactic Acid Production Using Sweet Potato Processing Waste, Irene Pagana

Graduate Theses and Dissertations

Organic waste generated from industrial sweet potato canning is estimated to be 30% of incoming raw material. This waste contains carbohydrates (sugars and starch) that could be used as substrates for the production of useful compounds via fermentation (e.g. lactic acid), resulting in the production of value-added products. The goal of this research project is to produce a substrate from the sweet potato processing waste material that supports the growth of lactic acid bacteria, which results in the production of lactic acid. The sweet potato waste product was characterized, and found to contain 16.5% solids. The solids components were 18.5% …

Investigations Of Protein-Lipid Interactions In Model Membranes: Influence Of Aromatic Anchoring Residues And Buried Polar Residues, Nick Gleason

Graduate Theses and Dissertations

To investigate in detail the interactions between transmembrane proteins and the lipid bilayers in which they are constituted, designed model peptides with selective isotopic labels were synthesized and analyzed by means of solid-state deuterium NMR spectroscopy. Starting from the well-characterized model peptide GWALP23, acetyl-GGALW(LA)6LWLAGA-amide, several Trp to Tyr mutations were compared to evaluate their respective interfacial anchoring abilities. It was found that Tyr, substituted on either or both termini, can effectively anchor the transmembrane alpha-helix, which then adopts a similar transmembrane topology in a range of bilayer thicknesses. Nevertheless, a consistent ~10° shift in tilt direction (helix rotation) is observed …

Superfund Evaluation: The Families Of Tar Creek, Gary D. Wilson

Graduate Theses and Dissertations

The Tar Creek Superfund Site is located in far northeastern Oklahoma near the Oklahoma/Kansas border in Ottawa County. The Site generally consists of a forty-square-mile area which is also part of the larger Tri-State Mining District that includes areas of Kansas and Missouri. The Site includes parts of five communities: Picher, Cardin, Quapaw, North Miami, and Commerce in Oklahoma. It also includes Treece, Kansas, and affects a total population of roughly 30,000 residents. Following Superfund designation, families in the Tar Creek area had to decide whether to accept or reject buy-out offers. This project explored the decision-making processes employed by …

The Evolution Of Natural Plant Communities Through Crop Migration And Crop-To-Weed Gene Flow, Meredith G. Schafer

Graduate Theses and Dissertations

With the rising demand for food and biofuels, the efficiency of crop production has become vital to assuring food security. Genetically engineered (GE) crop varieties are broadly thought to be a solution for improving the efficiency of crop production, but concerns regarding their release have heightened as more discoveries have focused on the influence of genetically engineered crops on native and weedy plant species. Risk assessment of GE crop varieties focuses on two main areas: feralization of crop systems and crop-weed hybridization. Risks include naturalization, transgene introgression, and the formation of novel genotypes in natural systems. In this study, roadside …

Interactions Between The Thermic Effect Of Food And Exercise On Total Energy Expenditure In Moderately Active Females, Ashley Binns

Graduate Theses and Dissertations

Diet and exercise are key components to overall health maintenance. Aspects contributing to weight maintenance include the thermic effect of food (TEF) and total energy expenditure (TEE), with TEF contributing to upwards of 10% TEE. PURPOSE: The purpose of this study was to determine the interaction between TEF and exercise on TEE in moderately active females. METHODS: A sample population was established with the use of a health history questionnaire (HHQ). Ten active females of normal body composition, determined by body mass index (BMI) and a dual energy absorptiometry x-ray (DEXA) scan, participated in the study. Maximal aerobic …

Peptoid Based Slide Coatings For Disease Detection Via Elisa Microarray Analysis, Melissa Lea Hebert

Graduate Theses and Dissertations

Poly-N-substituted glycines (peptoids) are a very versatile family of synthetic molecules that can be customized for any number of applications. In this study, we chose to use peptoids as a foundation for sandwich ELISA microarray analysis with a long term goal of creating an early detection device for complex diseases such as cancer. The peptoids were designed to self-assemble into microspheres to be used in coatings on the surface of the microarray substrates to increase the surface area available for antibody attachment. This increased antibody density would lead to an increase in the microarray analysis sensitivity and dynamic range. Studies …

Maternal Environment And Fescue Cultivar Effects On Growth, Development, And Fertility Of Beef Heifers, Jennifer Denyse Patterson

Graduate Theses and Dissertations

Eighty Brahman-influenced cows (5.6 BCS) were assigned to graze common bermudagrass (CB) or toxic tall fescue (E+) during a 60-d breeding season. Body condition (BC) was assessed at d 0, 30, and 60 of breeding season. Cows were managed to achieve marginal (4.2 BCS) or good (6.3 BCS) BC during last trimester. Heifer birth and weaning (WW; 7 to 8 mo) weights were recorded. The Angus sired heifers were weighed and randomly assigned to E+ or non-toxic (Novel) tall fescue at 9 to 10 mo of age for 190d. Heifer weight, growth data, and antral follicle count (AFC) were collected …

Comparative Response Of Different Broiler Genotypes To Dietary Nutrient Levels, Franco Javier Mussini

Graduate Theses and Dissertations

ABSTRACT

Three studies were conducted to evaluate how the broiler has changed over the years due to intense genetic selection. Four different broiler genotypes, one unselected since the 1950's (HER) representing the old meat-type bird and three current strains (Ross 308, Ross 708, and a test product (TY)) were studied. Differences in growth, morphometry, and response in performance and processing yield to different nutrient levels were studied.

Experiment 1 evaluated growth and morphometric analysis. Birds of each strain were killed weekly from day 7 to day 56, necropsied and cut up parts and organs weighed. Measurement of small intestine segments …

Evaluation Of Isothiocyanates And Herbicide Programs As Methyl Bromide Alternatives For Weed Control In Polyethylene-Mulched Tomato And Bell Pepper, Pratap Devkota

Graduate Theses and Dissertations

Methyl bromide (MeBr), a Class I ozone –depleting substance, has been banned for ordinary agricultural uses. In the absence of an effective MeBr alternative, weed control is a major challenge for commercial tomato and bell pepper production. Field trials were conducted at Fayetteville, AR, to compare allyl isothiocyanate (ITC), metam sodium, and herbicide programs with the standard MeBr application (mixture of MeBr plus chloropicrin at 67% plus 33%, respectively, hereafter referred to as MeBr) for crop injury, weed control, viable yellow nutsedge tubers, and marketable yield in low–density polyethylene (LDPE) mulched tomato and bell pepper production. In addition, herbicide programs …

The Use Of Cortisol And Hba1c As Biomarkers Of Stress In University Administrators, Jacilyn Marie Olson

Graduate Theses and Dissertations

PURPOSE: The purpose of this study was to explore the impact of perceived stress and cardiorespiratory fitness on cortisol and HbA1c, biomarkers of stress, in a group of select university administrators. The impact of gender on these relationships was of special concern. METHODS: University administrators with job titles of Chancellor, Provost, Vice Chancellors and Vice Provosts of the university, and Deans and Associate Deans at the college level were recruited. Twenty-five administrators (15 males and 10 females) agreed to participate and completed a battery of assessments that included completion of the University Administrative Concerns Questionnaire, a finger-stick blood test for …

The Identification Of Quantitative Trait Loci For Sucrose And Stachyose Content In Soybean Seed, Ailan Zeng

Graduate Theses and Dissertations

Sucrose is a desirable sugar in soybean seed that affects the quality and taste of soyfoods, while stachyose is a non-digestible sugar that induces flatulence in non-ruminant animals. Therefore, soybean cultivars with high sucrose and/or low stachyose would be valuable for soyfood and meal markets. The objectives of this study were to identify quantitative trait loci (QTL) or genes associated with seed sucrose and stachyose content using simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, PI 243545, to develop the sucrose QTL mapping population. A …

Consumer Sensory Preferences And Willingness-To-Pay For Nutraceutical-Rich Fruit, Lydia Lawless

Graduate Theses and Dissertations

Health-oriented juices are prime candidates for the nutraceutical market due to their inherent healthfulness and convenience. Fruits such as açaí berries, blueberries, blackberries, black cherries, Concord grapes, cranberries, and pomegranates are rich in potentially health-supporting polyphenolics, which makes the juices of these fruits attractive for nutraceutical optimization. The compositional, sensory, and stability properties of blueberry, blackberry, and Concord grape juice blends were explored in an extended shelf-life study. Initially, 100% blueberry juice had the highest total monomeric anthocyanins (67.40 mg/100mL), 100% blackberry juice had the highest total phenolics (249.24 mg/100mL), and 100% Concord juice had the highest polymeric color (23.21%). …

A Reexamination Of The Eastern Collared Lizard (Crotaphytus Collaris Collaris) In Arkansas, Ashley A. Grimsley

Graduate Theses and Dissertations

Loss of suitable habitat is a threat to species worldwide. Habitat destruction, including loss, change, and fragmentation of habitat, is the leading cause of species extinction. Eastern collared lizards (Crotaphytus collaris collaris) are habitat specialists on glades. Both C. c. collaris and glade habitats are rare and of special concern in the state of Arkansas. Many glade populations have already been extirpated in the Ozarks of Arkansas and Missouri. Increasing knowledge of the distribution, habitat structure, and population dynamics of C. c. collaris is important to ensure the survival of this species in Arkansas.

A literature review of …

Determination Of Methionine And Lysine Requirements Of Growing Broilers Using The Ideal Protein Concept, Changji Lu

Graduate Theses and Dissertations

Three experiments were conducted to evaluate the responses of broiler performance to Lysine (Lys) and Methionine (Met) during the starter, grower, and finisher feeding phases. All the experimental diets were formulated based on the Ideal Protein Concept.

Experiment 1 evaluated the responses to Lys and Met on live performance of young broiler chickens from 0 to 18 d of age. The mean amino acid ratios to Lys suggested by literature values were used in the formulation based on the Ideal Protein Concept. Six levels of Lys and eight levels of supplemental Met were used in the diets resulting in a …

Single-Pass Drying Of Rough Rice Using Glass Transition Principles, George Owira Ondier

Graduate Theses and Dissertations

The objective of this research was to study the drying characteristics, milling quality, and functional properties of rough rice subjected to single-pass drying while controlling kernel material states. Drying experiments were conducted at 60, 70, and 80C and relative humidities ranging from 13 to 83%. High drying air relative humidities (greater than 63%) maintained both the kernel surface and core in a rubbery state during drying, whereas low drying air relative humidities caused rapid transitioning of the surface layers from a rubbery to a glassy state. Long-grain pureline cultivar, Wells, medium-grain pureline cultivar, Jupiter, and long-grain hybrid cultivar, CL XL729, …

Flight Period And Species Composition Of Sirex (Hymenoptera: Siricidae) And Associated Deladenus (Nematoda: Neotylenchidae) Within Arkansas Pine Forests, Danielle Keeler

Graduate Theses and Dissertations

The European woodwasp, Sirex noctilio F. (Hymenoptera: Siricidae), which is a known destructive pest of pine in the southern hemisphere was recently discovered in the eastern United States. Before we can understand how S. noctilio may affect pine forests throughout the United States, we need a better understanding of native Sirex and the role they play in the ecosystem. The objectives of this research were to 1) determine species composition and flight period for native Sirex; 2) confirm presence of parasitic nematodes, Deladenus (Nematoda: Neotylenchidae), within adult Sirex; 3) investigate Deladenus parasitism rates; 4) verify the number of Deladenus species …

Genotypic And Phenotypic Diversity Of Pyricularia Oryzae In The Contemporary Rice Blast Pathogen Population In Arkansas, Lu Zhai

Graduate Theses and Dissertations

Rice blast, caused by Pyricularia oryzae (teleomorph: Magnaporthe grisea), is one of the most economically important diseases of rice worldwide, including Arkansas. Rice blast has been severe the past few years on conventional cultivars and, more recently, has been observed on hybrid rice cultivars. The first objective of the current research was to examine the genotypic and phenotypic variation in the P. oryzae population in Arkansas during the 2009, 2010, and 2011 growing seasons and compare isolates from conventional cultivars with those from and hybrids. A total of 904 isolates were recovered from symptomatic rice cultivars in Arkansas and …

The Use Of Diatoms To Infer Environmental Change Of The Lower White River, Southeastern Arkansas, Ruchi Bhattacharya

Graduate Theses and Dissertations

This dissertation describes the limnology of the lower White River oxbow lakes in southeast Arkansas, inundated by the major 2008 spring flood. The flood water and sediment deposited in oxbow lakes were analyzed for water chemistry, sediment geochemistry and diatom assemblages and used as an analogue for past floods. The 2008 spring flood inundated and homogenized the entire floodplain; also represented by the predominantly planktonic diatom assemblage suggesting riverine input. Indicator species for eutrophication were also recorded consistent with the high nutrient content in the lakes. However, the variance in the surface diatom assemblage was best explained by the lake …

Genome-Wide Identification Of Conditionally Essential Genes In Salmonella Typhimurium Using Tn-Seq Method, Anita Khatiwara

Graduate Theses and Dissertations

ABSTRACT

As more whole genome sequences become available, there is an increasing need for high-throughput methods that link genes to phenotypes and facilitate discovery of new gene functions. The objective of this study was to develop a high-throughput method to study gene functions in bacteria and use this method to study gene functions of S. enterica serotype Typhimurium (S. Typhimurium) under various environmental conditions encountered during its life cycle. Chapter I of this dissertation reviews the history and evolution of functional genomics in bacteria with focus on Salmonella, along with the recent techniques available. Chapter II, deals with the development …

Establishment Method And Cultural Practice Effects On Sports Turf, Josh Anderson

Graduate Theses and Dissertations

The use of soil-based sod on sand-based rootzones for the establishment of sports fields and golf courses is a common occurrence. Furthermore, the use of soil-based sod on sand-based rootzones can lead to potential problems associated with decreased water infiltration, gas exchange, rooting, and turfgrass quality caused by soil layering. Core-aerification and sand topdressing may help alleviate the problems associated with soil layering from the use of soil-based sod on sand-based rootzones. Therefore, the objectives of this research were to evaluate the effects of sod establishment methods and post- establishment cultural practices on the infiltration rate, moisture content, divot resistance, …

Investigation Of A Locus On Chromosome 9 For Contributions To Pulmonary Hypertension Syndrome In Broilers, Sriram Krishnamoorthy

Graduate Theses and Dissertations

Although the ascites syndrome in chickens has been investigated for years, it continues to inflict financial losses to the world poultry industry. It is estimated that 8% of the 361 million broiler deaths are due to ascites leading to losses of millions of dollars annually. Efforts to curb the incidence of ascites are typically designed to slow early growth. This limits the birds' ability to show its true genetic potential and impacts later yields. In 1994 lines divergent for susceptibility to ascites were established from a commercial sire line through sibling selection of birds reared at local altitude after testing …

Characterization Of Novel Histone H2b Mutants Associated With Chromosome Segregation Defects In Saccharomyces Cerevisiae, Thiruchelvam Rajagopal

Graduate Theses and Dissertations

Histones are small basic proteins that associate with DNA to form the basic unit of chromatin, the nucleosome. Histones H3 and H4 form a tetramer that is bound by two H2A-H2B dimers to form the histone octamer, to which approximately 146 bp of DNA wrap around to form the nucleosome. High resolution structural information and recent advances in the understanding of histone post-translational modifications have illuminated the many regulatory functions chromatin exerts in the cell, from the transcriptional control of gene expression to chromosome segregation. However, the specific role that histones play in these processes is not well understood. Previous …

Multifunctionality In U.S. Rice Production: A Logit Analysis In Farmers Participation, Juan Tur Cardona

Graduate Theses and Dissertations

Multifunctional agriculture is particularly fundamental to some working lands conservation policies and programs, such as the Environmental Quality Incentive Program (EQIP), Conservation Security Program (CSP) and Wildlife Habitat Incentive Program (WHIP). Farmers can also be engaged in providing recreational and agri-tourism services such as hunting, fishing, bird-watching, farm tours, petting zoos and hospitality services. Using the Agricultural Resource Management Survey (ARMS) we analyze factors associated with participation in conservation, recreation and agri-tourism activities as a function of farm structure, farm financial measures, production practices, and socio-demographic characteristics of the farm operator. To estimate the functional relationships we estimate a binary …

Interactions Of Nitric Oxide And Superoxide Pathways In Hyperglycemic Endothelial Cells, Steven Clay Rogers

Graduate Theses and Dissertations

Cardiovascular complications arising from diabetic hyperglycemia represents one of the leading causes of death and greatest public health challenges of modern societies. Despite state-of-the-art glucose control, diabetic patients remain at a markedly increased risk of cardiovascular disease. The loss of endothelial function (the development of diabetic endothelial dysfunction) has been implicated in the development of numerous diabetic cardiovascular diseases. The endothelial cell produces many vasoactive substances, hormones and cytoprotective biological factors. Endothelial cells are also involved in and affected by the initiation of inflammatory responses through the release and interaction of cytokines and other immune system molecules. Therefore, regulation of …

A Role Of Environmental Components In Autoimmune Vitiligo Of Smyth Line Chickens, Lei Dong

Graduate Theses and Dissertations

The Smyth line (SL) of chickens is a well-established animal model for the study of spontaneous autoimmune vitiligo, displaying all the characteristics of human vitiligo. In humans, phenolic derivative 4-tertiary butyl phenol (4-TBP) has been shown to trigger vitiligo in susceptible individuals. In vitiligo-prone SL-chickens, live turkey herpesvirus (HVT) infection at hatch was identified as the most effective trigger of SL-vitiligo expression. This dissertation examined the role of the environmental factors 4-TBP and HVT in the etiology of SL-vitiligo. Specifically, the generation of reactive-oxygen-species by melanocyte-containing feather tissue (MC-FT) and cultured melanocytes in response to 4-TBP exposure in vitro (Study …

Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam

Graduate Theses and Dissertations

The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …

A Comparative Analysis Of Free Trade Between The U.S. And The Eu : A Case Study Of The U.S./Chile Fta And The Eu/Chile Fta, Misti Dawn Sharp

Graduate Theses and Dissertations

Unlike many studies that assess the impacts of free trade agreements (FTAs), this thesis is a study of the negotiated outcomes in FTAs between the United States (U.S.) and Chile, and the European Union (EU) and Chile. Existing negotiation literature pertains to multilateral trade talks, dispute settlements or bilateral one good trade agreements. Studies regarding the negotiation process and other factors influencing outcomes in comprehensive FTAs are rare. In order to address this gap in the literature, this thesis is an analysis of how and why the FTA between Chile and the U.S. differs from the FTA between Chile and …

The Correlation Between Hip Abductor Strength And Functional Performance In Division I Female Collegiate Soccer Athletes, Caitlin Elizabeth Whale

Graduate Theses and Dissertations

Division I female soccer athletes have been documented to succumb to the greatest amount of lower extremity injuries, predominately ACL injuries. The risk for a lower extremity injury becomes greater when alterations to functional biomechanics are present. Specifically, the hip abductor strength has a crucial role in maintaining pelvic alignment. The purpose of this study was to determine if the hip abductor strength of a female athlete alters' biomechanics during functional performance. Thirty-two Division I female soccer athletes participated in this study (20+2yrs, 64.5+16.5kg, 166+14cm). Descriptive and anthropological statistics were recorded prior to participation. The athletes' isometric hip abductor strength …

Distribution And Control Of Herbicide-Resistant Italian Ryegrass (Lolium Perenne L. Ssp. Multiflorum Lam. Husnot) In Arkansas, James Walker Dickson

Graduate Theses and Dissertations

Italian ryegrass populations have evolved resistance to herbicides that producers rely on for weed control both in wheat and burn-down. The objectives of this research were to: test populations of Italian ryegrass from across Arkansas for resistance to glyphosate, diclofop, pinoxaden, and pyroxsulam; determine if there were any differences in control of 12 glyphosate-resistant populations in relation to glyphosate rate or application timing; determine the level of glyphosate resistance in one selected population versus a susceptible standard and a previously discovered glyphosate-resistant population; and determine the best options for controlling Italian ryegrass prior to planting crops. A total of 215 …

Identification And Confirmation Of Ssr Markers And Qtl For Seed Calcium Content And Hardness Of Soybean, Moldir Orazaly

Graduate Theses and Dissertations

The health benefits and the nutritional values of soyfood products have expanded the demand for food-grade soybean varieties. For whole soyfood products such as natto, small seeded varieties with proper texture are essential. Seed calcium content and hardness are main factors in determining the seed texture. Utilizing molecular markers associated with quantitative trait loci (QTL) for marker assisted selection (MAS) has been an efficient technique for breeders. To find molecular markers associated with a trait is an initial step. The confirmation of QTL in different environments and in different genetic backgrounds is important for MAS. The objectives of this research …