Life Sciences Commons^™

Bacterial Chondronecrosis With Osteomyelitis In Broilers: Etiology And Selected Mitigation Measures, Khawla Saud Alharbi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis (BCO) is the predominant cause of lameness in broiler chickens. It mainly impacts broiler production and animal welfare, causing substantial financial losses accounting for 1- 2% due to bird condemnations at the marketing age. Remediating BCO lameness disease demands investigating its causes using appropriate models and evaluating preventive measures, such as enhancing epithelial tissue health and tight junction integrity in the digestive and respiratory tract, strengthening immune functions, and improving bone strength. Therefore, the objectives of this dissertation were to 1) study predisposing factors for BCO lameness by investigating the effects of deoxynivalenol (DON) and fumonisin …

Polyphenol-Protein Interactions In Pigmented Cereals – A Clean-Label Approach To Tailor Starch Viscosity, Annegret Jannasch

Graduate Theses and Dissertations

Viscosity plays an important role in the food industry as it affects the quality, processing stability, and shelf-life of food and impacts physiological responses such as satiation and satiety. Greater viscosity reduces food intake by increasing the perception of satiety, primarily by delaying gastric emptying and increasing tactile stimulation. Starch is commonly added to food products as a viscosifying ingredient, which often involves chemically modifying the starch via cross-linking, to enhance its resistance to high shear, temperature and acidity. However, chemically modified starches are not “clean-label” ingredients due to the use of toxic chemicals. Therefore, further exploration is needed to …

Impacts Of Toxic Fescue Seed Consumed By Pregnant Heifers On Maternal Blood Flow And Offspring Growth Parameters: Potential For Melatonin As A Therapeutic To Improve Offspring Growth, Rachel Scott

Graduate Theses and Dissertations

The southeastern United States is home to endophyte-infected (E+), toxic, tall fescue grass. Consumption of E+ fescue by livestock has been linked to fescue toxicosis, a disease reported to cost the United States beef industry around $1 billion in annual losses. The primary objective of this dissertation was to evaluate the influence of pregnant cattle consuming E+ seed during gestation on uterine artery blood flow and growth performance of female offspring. The secondary objective was to investigate melatonin supplementation as a mitigation strategy for expected fescue-associated decreases in uterine artery blood flow and offspring growth performance. The study reported in …

Gene Expression And Transfection Of C15orf57 In Chicken Show Clear Correlation To The Function Of The Ap2 Complex, E. Ashton Magdich

Graduate Theses and Dissertations

Discovering the functions of functionally unknown genes has made a huge impact on biological understanding including poultry species. In this thesis, C15orf57 (or C5H15orf57, chicken chromosome 5 human ortholog H15 open reading frame 57), was studied to understand its genetic characteristics and function. Chapter one is literature reviews on annotation of genes, the gene information on C15orf57, the importance of characterizing unknown genes in the genome, and what is known now about C15orf57. The Chapter 2 is for the structural characterization of C15orf57, gene expression of C15orf57 in various organ tissues using the qPCR method and effect of overexpression of …

Effects Of Cholesterol Concentration On Liposome And Proteoliposome Behavior, Ehsaneh Khodadadi

Graduate Theses and Dissertations

Cholesterol is crucial in modulating the behavior and characteristics of biological membranes, liposomes, and proteoliposomes, for instance in drug delivery applications. Cholesterol regulates membrane stability by influencing lipid density, phase separation, and protein-membrane interactions. In planar bilayers, cholesterol enhances membrane thickness, reduces permeability, and promotes lipid ordering, adding rigidity and fluidity. Cholesterol also modulates membrane curvature in curved bilayers and vesicles, stabilizing low-curvature regions. In this work, all-atom molecular dynamics (MD) simulations were employed to examine how varying cholesterol concentrations influence the structure and dynamics of membranes, focusing on planar and curved geometries. Bilayers with cholesterol molar ratios of 0%, …

Characterization Of Streptococcus Gallolyticus: An Emerging Pathogen Causing Turkey Streptococcosis, Latasha S. Gray

Graduate Theses and Dissertations

During the past five years, there has been an increased occurrence of Streptococcus gallolyticus in young turkey poults. This emerging pathogen previously affected turkey flocks without any indication of disease prior to acute mortality. However, recently there has been two cases of neurological presentation associated with S. gallolyticus subsp. pasteurianus that are unique to traditional disease presentation in turkey poults. The objectives of the present dissertation were to compile data associated with the two neurological cases and compare different S. gallolyticus strains as well as pathogenic Enterococcus cecorum to the neurological strains. The two neurological cases occurred between July and …

Utility Of John Deere's See & Spray Ultimate In Southern U.S. Row Crops, Tristen Avent

Graduate Theses and Dissertations

Row crop producers across the southern United States are observing a boom in technological advancement to improve operational efficiency. Additionally, environmental concerns and reduced profit margins drive the adoption of targeted pesticide application technologies like the John Deere See & Spray. This technology is relatively new, introduced in 2022, and producers need more insight into the shortcomings, potential herbicide savings, and the economic benefits of adoption. Since 2021, research has been conducted in Keiser, AR, with additional collaborative projects in Mississippi, North Carolina, Indiana, and Illinois. Five different objectives were addressed in this research: 1) to determine the impact of …

Crispy Not Risky: Effects Of Environmental Conditions On Low-Moisture Food Persistent Bacterial Pathogens, Manita Adhikari

Graduate Theses and Dissertations

Low-moisture foods (LMFs) were not historically seen as safety risks due to their ability to inhibit pathogen growth, but evidence shows pathogens can survive in LMFs long-term, causing outbreaks and recalls. Previous studies have lacked the ability to replicate real-world contamination scenarios in LMF processing environments. This research aimed to develop inoculation methods to form LMF persistent bacterial populations (LMF PBPs) and assess pathogen responses to environmental factors, culturing methods (broth vs. lawn), and matrix type and ratio. Inoculation methods tested were “direct” (combining bacterial culture and powder on a stainless-steel coupon) and “slurry” (premixing culture and powder). Matrices included …

Comparisons Between Traditional And Gluten-Free Cookies In Sensory Attributes, Acceptance, And Evoked Emotions, Eniola Ola

Graduate Theses and Dissertations

The rise in food allergies and intolerances, notably conditions like Celiac disease, as well as a general shift of consumers to a more health-conscious lifestyle has significantly increased the demand for gluten-free products. Consumers increasingly seek gluten-free products, yet these alternatives often face challenges in replicating the sensory qualities of traditional gluten-containing foods. This study explores the sensory attributes, emotional responses, and labeling impacts on consumer perception of gluten-free and gluten-containing cookies. The main objective of this thesis was to address the gap in research on sensory evaluation of gluten free foods, specifically in terms of commercially available products. The …

Bactericidal Mechanism Of Peracetic Acid Against Salmonella Mediated By Reactive Oxygen Species, Mohammed Khalid Wheib Al-Doury

Graduate Theses and Dissertations

Peracetic acid (PAA) is extensively used as a bactericidal agent in poultry processing to reduce foodborne pathogens, including Salmonella enterica serotype Typhimurium (S. Typhimurium). Despite its widespread application, the exact mechanisms underlying its bactericidal effects, particularly the role of reactive oxygen species (ROS), remain incompletely understood. This dissertation examines the role of ROS in PAA-mediated bacterial killing and explores both genetic and metabolic factors that influence bacterial susceptibility to PAA. Findings from these studies have implications for enhancing the efficacy of PAA, thereby improving food safety practices in the industry.

The first study investigates ROS as a central component …

Advancing Molecular Tools For The Study Of Rubus Viruses, Andrea Sierra-Mejia

Graduate Theses and Dissertations

Since blackberry yellow vein disease (BYVD) was first described, the blackberry virome has expanded significantly. BYVD is a complex disease associated with over ten viruses, yet the role of each virus in its etiology remains understudied. Studying the effect of each virus in disease development requires the identification of single infections; however, this task has been difficult for BYVD, with all field collected material over the past two decades consistently found infected by multiple viruses. Given the diversity of viruses associated with the disease and their different modes of transmission, obtaining pure virus cultures necessitate transmission of individual viruses via …

Effects Of Fast And Slow-Wilting Soybean Genotypes On Plant-Herbivore Interactions, Jessica Ayala

Graduate Theses and Dissertations

Soybean [Glycine max (L.) Merr.] is one of the most important food crops in the world due to its multiple uses in agriculture sector. A major limiting factor in soybean production system is drought stress, causing up to 80% reduction in yield. Due to climate change and associated rise in ambient temperatures, water scarcity is a major concern in crop production, and consequently researchers are turning to soybean accessions that demonstrate better water use efficiency (WUE) traits. However, field grown soybean is also under severe pressure from insect herbivores, often accounting for a 30% reduction in yield. Among these, …

Encapsulation Of Probiotics By Cellulose Nanocrystal-Alginate Composite Beads For Ph Stimuli-Responsive Delivery, Akrip Jati

Graduate Theses and Dissertations

Microencapsulation has garnered significant interest, with oral probiotics delivery systems showing enormous potential to help restore the gut microbiome. This method holds vast potential to protect and release specific microbial strains to the desired target site (i.e., colon) within the gastrointestinal tract (GIT). However, current encapsulation methodologies, utilizing either synthetic or biological materials as carriers, encounter challenges such as suboptimal encapsulation efficiency, premature probiotic release, and inadequate control over release kinetics. In this work, hydrogel microsphere (HM) composites of different cellulose nanocrystal (CNC) forms (i.e., colloidal (c), and crosslinked (x)) and alginate (ALG) were used as advanced controlled release systems …

Comparison Of Statistical And Machine Learning Genomic Prediction Methods In Plant Breeding: Case Studies In Maize And Soybean, Igor Kuivjogi Fernandes

Graduate Theses and Dissertations

Plant breeding is essential to increase genetic gain and food production worldwide. This study was conducted to evaluate new ways to use machine learning (ML) to tackle plant breeding challenges, where two ideas were tested — the first chapter focuses on how to combine genetic and environmental data using ML to improve the prediction of maize grain yield in multi-environment trials, while the second chapter centers on how to couple feature selection of molecular markers with ML to enhance prediction of yield in soybean, and, in both cases, ML approaches were compared to well-established statistical methods greatly adopted by the …

Neural Network Architecture Search Enabled Wide-Deep Learning (Nas-Wd) Integrated Hyperspectral Imaging Understanding For Woody Breast In Poultry Processing, Chaitanya Kumar Reddy Pallerla

Graduate Theses and Dissertations

The development and implementation of a Wide & Deep (WD) learning model tailored for classification and regression tasks utilizing spectral data provides a robust solution to evaluate woody breast (WB) conditions in poultry fillets. This process begins with thorough data preprocessing, which includes loading spectral and classification datasets, imputing missing values with medians, and splitting the data into training and testing sets to ensure rigorous model evaluation. The WD model architecture integrates wide linear models and deep neural networks to harness the strengths of both approaches. The wide component excels at memorizing sparse feature interactions, while the deep component captures …

Investigating Consumer Perception And Wine Quality Impact On Alternative Packaging On Grape (Vitis), Mark Walker Bartz

Graduate Theses and Dissertations

The grape (Vitis species) and wine industry has major global economic impacts but faces issues with sustainability. The use of glass bottles for wine production is environmentally unsustainable due to energy-intensive manufacturing and poor recycling rates. However, wine consumers tend to associate glass bottles with higher quality over alternative wine packaging materials such as aluminum, polyethylene terephthalate (PET), and flexible bags/pouches. Since wine price and packaging influence consumers perception of wine quality, wine producers need to understand consumers to maximize profits. Thus, the overall objectives of this research were to investigate consumer perception and wine quality impact of alternative …

Enhancing Selection Intensity With High-Throughput Phenotyping Data In Plant Breeding, Ashmita Upadhyay

Graduate Theses and Dissertations

Genomic prediction (GP) has proven to be an essential tool to accelerate the cultivar development pipeline by predicting the performance of un-phenotyped lines in the field of plant breeding. However, the prediction accuracy is bounded by the heritability of the target trait, limiting the efficiency of the single-trait GP model. To overcome the limitation, a multi-trait genomic prediction model using high-throughput phenotyping (HTP) data can be used to leverage secondary traits to boost the predictive ability of the target trait. This study aimed to assess the efficiency of the multi-trait (MT)- GP model powered by HTP derived secondary traits in …

Blend Matters: Interactions Of Rice Cultivars On Milling, Physico-Chemical & End Use Traits, Bindu Regonda

Graduate Theses and Dissertations

Rice blending or mixing of different rice cultivars, a common practice in the industry, significantly impacts processing efficiency and end-product quality, yet its effects on end products remain understudied. This research investigated the implications of blending contemporary Arkansas rice cultivars through two comprehensive studies focusing on milling yields and physicochemical properties, instantization, and parboiling processes. Five long-grain cultivars, including hybrids (RT 7521 FP, RT 7321 FP, XP 753) and purelines (Ozark, CLL 16), were blended in various combinations and different drying.

The first study revealed that blending significantly affected milling yields and physicochemical attributes. Pre-drying blending yielded better results compared …

The Impact Of Wild Pigs (Sus Scrofa) On Avian Occupancy And Vegetation Structure, Kenneth Wilson

Graduate Theses and Dissertations

Invasive species, particularly wild pigs (Sus scrofa), pose a significant threat to native ecosystems through both direct and indirect impacts. Wild pigs are large-bodied omnivorous species that can consume a wide variety of plants and animals and are known for destructive foraging behaviors, which likely threatens avian communities through habitat alteration and can impact the long term structure of ecosystems where they are present.

In chapter 1, we used a combination of avian point count surveys, vegetation assessments, and camera trap surveillance to quantify how the occupancy of breeding birds in bottomland hardwood forests changed in relation to …

Utilization Of Prosur Phr As A Novel Phosphate Alternative In Tumble, Inject, And Tumble Injection Marinated Chicken Breast, Sarah E. Johnson

Graduate Theses and Dissertations

Poultry is the most widely consumed meat protein worldwide for its health consideration, affordability, and versatility. Historically, the poultry industry has utilized numerous methods to improve meat quality most significantly, marination. Marination is practice commonly used in the industry by imparting various chemical functional ingredients that are known to enhance sensory characteristics such as tenderness, juiciness, and flavor. Various marination methods used in the industry including tumble, injection, and soaking. Ingredients, such as sodium chloride and phosphates are commonly used in the marination process. In recent years the industry has witnessed a shift in consumer preferences to “clean-label”. This trend …

The Neural Mechanisms Of Visual Art Expertise During Different Stages Of The Creative Process: An Eeg Study, Kim N. Awa

Graduate Theses and Dissertations

Generating and drafting ideas are creative processes linked to internal neural mechanisms, such as directed attentional states, reflected in alpha band synchronization patterns. Previous research has demonstrated that these mechanisms vary based on levels of expertise during creative tasks. However, few studies have examined the multifaceted nature of ideation and externalization across multiple stages of the creative process, particularly when accounting for domain-specific expertise in imagery and figural tasks. The present study investigates alpha band synchronicity (lower and upper) across visual art backgrounds, specifically among artists, intermediates, and non-artists, during three distinct stages of the creative process: thinking of ideas, …

Cognitive Bias, Memory, And Fearfulness Of Red Jungle Fowl And Athens Canadian Random Bred Chickens, Victor Juwon Oyeniran

Graduate Theses and Dissertations

Our understanding of how poultry perceive and interpret their environment is essential to enhancing their welfare; over the years, animal welfare science has relied on measures of the behavioral and physiological components of affective states (positive and negative) as welfare indicators. There is an increasing interest in the study of chicken’s affective states using the Judgment bias test (JBT) as a tool for assessing welfare through their responses to ambiguous cues. This study investigates the cognitive performance of two chicken breeds with different evolutionary histories: Red Junglefowl (RJF), the primary ancestor of modern chickens, and Athens Canadian Random Bred (ACRB), …

Frogs On A Plain: Spatial Ecology Of Crawfish Frogs In Arkansas’ Remnant Prairies, Elliot T. Lassiter

Graduate Theses and Dissertations

The prairie peninsula was an expansive and important habitat that covered much of midwestern North America, from Canada to Arkansas, USA. But the prairie peninsula has shrunk by as much as 99% since 1830, and therefore, land acquisition and public/private partnerships are critical for the preservation of prairies and their associated species. In Arkansas, one species that benefits from protected prairies is the Crawfish Frog (Lithobates areolatus), which is currently declining precipitously throughout its range. Adult Crawfish Frogs spend most of their lives in terrestrial refugia (crayfish burrows), making them highly vulnerable to habitat loss and fragmentation, thereby …

Influence Of Hydrologic And Anthropogenic Disturbance On Stream Populations And Communities, Sarah Frances Sorensen

Graduate Theses and Dissertations

Disturbances occur when an external force modifies a habitat, destroying living space, removing food resources, or altering or destroying habitat structure. In lotic systems, a primary source of disturbance is hydrologic disturbance, including drought and flood. Hydrologic disturbances lead to increased mortality rates and dispersal out of disturbed habitats, causing population sizes to decrease and changes to community structures. More research is needed on how disturbances impact stream species and communities, as disturbances are predicted to become more intense with climate change. This dissertation aims to understand how disturbance influences streams at both the population and community level, through a …

Integrating Sustainability And Food Safety Through Quantitative Microbial Risk Assessment And Life Cycle Assessment Of Low-Moisture Foods, Jessica Bain

Graduate Theses and Dissertations

Food safety and sustainability are critical concerns in the food industry. However, these areas are often evaluated separately, leading to an incomplete understanding of the overall impact of food production on human health. This dissertation aims to integrate food safety with sustainability to provide a comprehensive assessment of the impact of low-moisture foods on human health, using the almond processing supply chain as a case study.

In addition to conducting life cycle assessments (LCA) of almond processing, wheat flour production, and dried apple processing, this dissertation aims to create a framework to bridge the gap between sustainability and food safety. …

Analyzing Milled Rice Breakage Patterns Under Diverse Environmental Settings, Devisree Chukkapalli

Graduate Theses and Dissertations

This study comprehensively evaluates the effects of environmental conditions, transportation, and storage practices on the quality of milled rice using three cultivars: CLL16 (long grain pureline), XP753 (long grain hybrid), and Titan (medium grain pureline). Three distinct objectives guided the research: (1) assessing the impact of relative humidity (RH), temperature, and exposure duration on rice quality post-milling and post-storage; (2) analyzing seasonal and locational influences on rice quality during transportation and storage; and (3) investigating the effects of subfreezing storage on key quality attributes.
Under controlled environmental conditions, post-milling exposure (PME) caused significant quality degradation at higher RH (75% and …

Diseases Of Cannabis Sativa And The Epidemiology And Molecular Diversity Of Neofusicoccum Parvum, Hannah V. Zima

Graduate Theses and Dissertations

Cannabis sativa has been an underutilized agricultural resource in the United States, largely due to federal regulations distinguishing marijuana from hemp. In 2018, Congress passed a Farm Bill, which defined hemp as C. sativa containing a tetrahydrocannabinol (THC) level of no more than 0.3%. As a result, commercial hemp production began in most states in 2019, including Arkansas, and was accompanied by numerous challenges like diseases and pests. The research on the epidemiology of diseases affecting C. sativa is limited, and understanding the pathogens is in its early stages in the U.S. For this study, an initial disease survey was …

Detection Of Fungicide Resistance In Population Of Corynespora Cassiicola From Arkansas Soybean And Cotton Fields, Rafael Zaia

Graduate Theses and Dissertations

Target spot, caused by the fungus Corynespora cassiicola, has reemerged since 2016 as an economically important disease on soybean and cotton across the Southern U.S. The quinone outside inhibitors (QoI) and succinate dehydrogenase inhibitors (SDHI) are important fungicide classes used to control foliar diseases in soybean and cotton. The efficacy of these products is rapidly decreasing, impacting control, and causing significant yield losses. The overall objective of this study was to determine the risks associated with chemical control by screening fungicide sensitivity, evaluating target site alterations, and analysis of disease control efficacy and yield response of fungicides for soybean in …

Interactive Effects Between Bluetongue Virus Infection And Insecticide Exposures In Culicoides Sonorensis, Parker Mullins

Graduate Theses and Dissertations

Culicoides biting midges are common pests around the world of humans, livestock, and wildlife. In addition to nuisance blood feeding, they are also able to transmit pathogens to their hosts like bluetongue virus (BTV) and African horse sickness virus, which can be damaging in terms of both production economics and animal welfare. In order to protect humans and livestock from biting pests like Culicoides, a wide array of interventions is recommended, but chemical insecticides and repellent use are ubiquitous. For a multitude of reasons ranging from natural weathering of applied insecticides to the increase in resistance-associated mutations and behaviors in …

Fungal Pathogen Control And Microbial Ecology: Insights From Soybean Seed Production, Samantha Rigo Segalin

Graduate Theses and Dissertations

This study evaluated the effects of planting date (PD), seed vigor, and chemical seed treatments on the plant stand and yield of two soybean cultivars with different maturity groups (MGs) across four locations in Arkansas from 2018 to 2021. Early planting in early May (early PD) led to higher plant stands and yields compared to late planting in early June (late PD). The interaction between planting date and cultivar was significant, with both cultivars showing higher plant stands on early planting dates. Seed vigor and seed treatment also significantly impacted plant populations; high-vigor seeds planted early resulted in better stands …