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Articles 1141 - 1170 of 1741
Full-Text Articles in Life Sciences
Assessment Of The Level Of Protection Against Coccidiosis In Broiler Breeders Conferred By A Live Anticoccidial Vaccine, And Its Influence On Early Growth And Development., Muhammed Shameer Abdul Rasheed
Assessment Of The Level Of Protection Against Coccidiosis In Broiler Breeders Conferred By A Live Anticoccidial Vaccine, And Its Influence On Early Growth And Development., Muhammed Shameer Abdul Rasheed
Graduate Theses and Dissertations
Coccidiosis continues to be a great challenge to the poultry industry all over the world and in broiler breeders vaccination with live oocysts of Eimeria has been widely adopted as a control measure. Broiler breeders are usually subjected to feed restriction during their early growth however no studies have been undertaken on the effects of vaccination in birds whose feed has been restricted in this manner. The objective of this study was to assess the level of protection acquired by broiler breeder chickens when immunized at day-old with live eimerian oocysts and given a restricted diet. Effects upon growth and …
Effect Of Hydration Status On Thermoregulatory Responses In Non-Obese And Obese Males, Matthew Tucker
Effect Of Hydration Status On Thermoregulatory Responses In Non-Obese And Obese Males, Matthew Tucker
Graduate Theses and Dissertations
During heat stress the human body thermoregulates via cutaneous vasodilation and sweating. Hypohydration can impair thermoregulatory responses that stem from the central nervous system (CNS), but it is unknown if impairments also occur post-synaptically in the microcirculation. Moreover, obese individuals may have impaired thermoregulation, possibly due to microvascular dysfunction. Purpose: The purpose of these studies was two-fold: 1) to determine if obese (OB) individuals exhibit impairments in thermoregulatory responses during exercise heat-stress (centrally-mediated) and intradermal infusion of vasoactive substances (peripherally-mediated) versus non-obese (N-OB), and 2) to determine if hypohydration subsequently affects these thermoregulatory responses differently between groups. Methods: Twenty-one healthy, …
Characterization Of Broiler Lines Divergently Selected For Breast Muscle Color, Sara Katherine Orlowski
Characterization Of Broiler Lines Divergently Selected For Breast Muscle Color, Sara Katherine Orlowski
Graduate Theses and Dissertations
An increase in the consumption of poultry has generated an increase in demand for higher yielding broilers. This has led to an increase in atypical meat and issues with appearance. Color is a direct result of a pH decline as meat goes through rigor mortis with meat generally becoming lighter. If the pH declines too rapidly or too slowly, meat quality can suffer. Physical properties of meat can be altered by pH. A fast pH decline results in pale meat with decreased tenderness. A slow pH decline can result in darker meat with a reduced shelf-life. With a known relationship …
In Vitro Fermentation Patterns Of Rice Bran Components By Human Gut Microbiota, Tung Minh Pham
In Vitro Fermentation Patterns Of Rice Bran Components By Human Gut Microbiota, Tung Minh Pham
Graduate Theses and Dissertations
The prevalence of colorectal cancer (CRC) has been increasing in recent years worldwide. In the United States, it is ranked the second leading cause of cancer death. Risks of CRC increase with age and are associated with several lifestyle factors such as diet, drinking and smoking habits, and levels of physical activity. There is an abundance of scientific literature demonstrating the protective roles of several dietary components including fibers and polyphenolic compounds. These compounds have been shown to be able to positively modulate gastrointestinal ecology by increasing the production of short-chain fatty acids (SCFA) and promoting the population of beneficial …
Mental Toughness, Grit, And Motivational Differences In Single-Sport And Multi-Sport Athletes, Cole Justin Shewmake
Mental Toughness, Grit, And Motivational Differences In Single-Sport And Multi-Sport Athletes, Cole Justin Shewmake
Graduate Theses and Dissertations
Whether it is at work, school or sports, mental toughness (MT), grit and motivation are essential to accomplishing goals. Sports can be an integral platform for developing MT, grit, and factors that increase motivation levels, and it is during childhood and adolescence that these characteristics may develop. All of this could prove beneficial into adulthood. Therefore, the purpose of this study was to examine MT, grit and motivational orientations of single-sport and multi-sport athletes at different levels of competition. Considering MT, a 2 x 2 ANOVA indicated no significant interaction effect between athlete type and competition level, F(1, 387) = …
Two-Electron Quenching Of Dinuclear Ruthenium(Ii) Polypyridyl Complexes, Yinling Zhang
Two-Electron Quenching Of Dinuclear Ruthenium(Ii) Polypyridyl Complexes, Yinling Zhang
Graduate Theses and Dissertations
A bridging ligand 5,5’-Bi- 1,10-phenanthroline, diphen, was prepared using dichlorobis(triphenylphosphine)Ni(II), Ni(PPh3)2Cl2 as catalyst with a yield of 40%. Yellow cubic crystals were able to obtain from the good purity product for single crystal analysis. The torsion angle between the planes of the subunit phenanthrolines is about 66 degrees.
A dinuclear ruthenium (II) polypyridyl complex, (phen)2Ru(diphen)Ru(phen)24+, was synthesized by using polymeric ruthenium carbonyl compound as the entry point, diphen as the bridging ligand and 1,10-phenanthroline, phen, as the terminal legand. Brown needlelike crystals were precipitated from acetonitrile that were not suitable for single crystal diffraction.
The photochemistry of the dimer was …
Comparisons Of Selected Household And Commercial Disinfectants Against Poultry Salmonella Isolates And A Survey Of Internal Parasites In Backyard Poultry In Arkansas, Kayleigh Moyle
Graduate Theses and Dissertations
Backyard and exhibition poultry have been gaining in popularity and as such there has been a large increase in the number of small flocks. As the interaction with poultry has increased, so has the opportunity for diseases and parasites, for both birds and people. One of the major zoonotic illnesses is caused by the bacteria Salmonella, which can be found in commercial and small flocks. Salmonella is the number 2 contributor of foodborne illnesses so its prevalence in commercial flocks is of high concern. Despite improved cleaning, disinfection, and biosecurity practices, there is still potential for disease outbreaks and infections …
Determination Of Optimum Fall And Spring Nitrogen Fertilizer Rate For Maximizing Grain Yield Of Soft Red Winter Wheat Sown At Variable Planting Dates, Lucas Vasconcelos Vieira
Determination Of Optimum Fall And Spring Nitrogen Fertilizer Rate For Maximizing Grain Yield Of Soft Red Winter Wheat Sown At Variable Planting Dates, Lucas Vasconcelos Vieira
Graduate Theses and Dissertations
An optimum planting date is important for winter wheat nitrogen (N) management as it dramatically changes the growing environment including temperature and moisture, ultimately affecting fertilizer efficiency and grain yield (GY). In Arkansas, high precipitation in the fall often forces farmers to delay planting and current Arkansas recommendations include the application of fall N when soft red winter wheat (SRWW) is sown later than optimum, despite the lack of data supporting this practice. This study evaluated the effect of rate and timing of N application on GY of SRWW sown at variable planting dates in Arkansas. Granular urea was split …
Shifts In Myxomycete Community Structure In Selected Microhabitats Across Nutrient Treatments In A Lowland Tropical Forest Of Panama, Laura M. Walker
Shifts In Myxomycete Community Structure In Selected Microhabitats Across Nutrient Treatments In A Lowland Tropical Forest Of Panama, Laura M. Walker
Graduate Theses and Dissertations
Myxomycetes (plasmodial slime molds) are abundant amoeboid predators of bacteria and other microorganisms. They are found worldwide, largely in association with decaying plant material in terrestrial habitats. Their consumption of bacterial prey puts microbial predators such as myxomycetes in a key position in various ecosystem processes wherein they help regulate the flow of nutrients (e.g., N and C) through the ecosystem. The importance of microbial predators in nutrient cycling and plant productivity is well established. Given the extent to which tropical ecosystems influence global nutrient fluxes, along with the ecologically significant role that myxomycetes play in these processes, there is …
Culinary Competence: Skills And Knowledge Assessment For Dietetic Students, Nancy Harris Buckley
Culinary Competence: Skills And Knowledge Assessment For Dietetic Students, Nancy Harris Buckley
Graduate Theses and Dissertations
Graduates of didactic programs in dietetics are entering internships with little cooking experience. The Food and Culinary Professionals’ Dietetic Practice Group (FCP DPG) has identified 11 core competencies that were developed as a recommendation to dietetic educators to include more culinary knowledge into existing programs. The purpose of this study was to determine if developing and incorporating experiential learning targeted to the competencies would increase dietetic students’ culinary skills and knowledge at a southern land-grant university. Food and Culinary Professionals’ DGP core competencies were used to guide the development of curriculum, experiential learning, and assessment in the Principles of Foods …
Investigation Of The Interaction Of Dimeric Ruthenium Complexes With Cytochrome B5, Christopher Dain Rupar
Investigation Of The Interaction Of Dimeric Ruthenium Complexes With Cytochrome B5, Christopher Dain Rupar
Graduate Theses and Dissertations
Photoreactive complexes to study the kinetics of electron transfer of proteins have been in use for a long time. It has always been speculated that complexes bind near the heme or the electron transfer reaction would not occur. But it is unkown exactly how the complex interacts with the protein. The structural, thermodynamic, and kinetic properties of rat liver microsomal cytochrome b5 were investigated when bound to ruthenium dimer complexes. Heteronuclear Single Quantum Coherence studies support a dynamic binding model of a dimer Ru complex bound near the protein’s heme involving residues H39, E44, G42, V61, G62, and H63. The …
The Cytotoxic And Antimicrobial Properties Of Pine Essential Oils: A Characterization And Comparison, Richard Sakul
The Cytotoxic And Antimicrobial Properties Of Pine Essential Oils: A Characterization And Comparison, Richard Sakul
Graduate Theses and Dissertations
In the forestry industry, the pine tree species are important because of their durable timber and fast growth. In Arkansas, trees such as the loblolly pine compose almost a third of the timberland, seven million acres. In addition to the lignocellulosic biomass, pine bark and needles potentially have industrial importance as a waste stream from which high value (e.g., pharmaceutical, cosmetics) chemicals could be extracted, which could potentially increase the profit margin of forestry operations. In this research, the possibility that pine needles harvested from industry processed pine tree residues could be used as an antibacterial or cytotoxic chemical agent …
Rice Consumption And Effects Of Rice Cake, Seolgitteok, On Human Health, Ellen Joy Pottgen
Rice Consumption And Effects Of Rice Cake, Seolgitteok, On Human Health, Ellen Joy Pottgen
Graduate Theses and Dissertations
Within the United States type 2 diabetes is an ever growing health epidemic. The prevalence in the adult population has quadrupled over the past 30 years and is expected to continue on a similar path in the coming decades. While the cause of type 2 diabetes is multifactorial, it is considered to be an acquired condition related to environmental contributors including poor diet, obesity, and physical inactivity, which may be managed to alter the course or progression of the disease. Preventative or maintenance measures emphasize nutritional intervention strategies, including encouraging individuals to follow a nutrient-dense, high-fiber diet with ample whole-grains, …
Efficacy Of A Novel Intranasal Zinc Solution On The Microbiome, Health, And Growth Performance Of High-Risk, Newly Received Stocker Cattle, Makenzie Foster
Efficacy Of A Novel Intranasal Zinc Solution On The Microbiome, Health, And Growth Performance Of High-Risk, Newly Received Stocker Cattle, Makenzie Foster
Graduate Theses and Dissertations
The objective of this study was to determine if using an intranasal zinc (Zn) solution would impact health and growth performance of high-risk stocker cattle. Male beef calves (n = 239; 3 arrival dates [block]; initial BW = 276 ± 2.4 kg) were stratified by arrival gender and BW and assigned to 1 of 2 treatments: 1) treated with 3 ml of a Zn solution containing 36.24 mg of Zn administered intranasally, or 2) control, in which calves were not treated. Calves were observed daily and if exhibiting signs of morbidity and a rectal temperature ≥ 40° C they were …
Microbes In Rough Rice – Prevalence, Growth Kinetics And Links To Dry Matter Loss, Supriya Thote
Microbes In Rough Rice – Prevalence, Growth Kinetics And Links To Dry Matter Loss, Supriya Thote
Graduate Theses and Dissertations
The prevalence of microorganisms on freshly-harvested long-grain hybrid, long-grain pureline, and medium-grain rice has not been elucidated. The first objective of this two-part study was to determine the relative prevalence of microbes on freshly-harvested, high moisture content rice of different types and cultivars. The total and surface aerobic plate counts (APC) and mold counts were determined in terms of log10 colony forming units per gram of rice (log10 CFU/g) for six rice cultivars over two years. In 2013, among harvested samples of all the studied rice cultivars, the long-grain hybrid cultivars had the lowest APC levels (M = 8.10, SD …
Stream Microbial Communities As Potential Indicators Of River And Landscape Disturbance In North-Central Arkansas, Wilson Howard Johnson
Stream Microbial Communities As Potential Indicators Of River And Landscape Disturbance In North-Central Arkansas, Wilson Howard Johnson
Graduate Theses and Dissertations
In the past decade, 29 shale basins have been actively developed across 20 states for extraction of natural gas (NG) via horizontal drilling/hydraulic fracturing (=fracking). This includes ~5000 wells within the Fayetteville shale of north-central Arkansas. Development often impacts both river- and landscapes, and management requires catchment-level evaluations over time, with organismal presence/absence as indicators. For this study next-generation sequencing was used to identify/characterize microbial communities within biofilm of eight Arkansas River tributaries, so as to gauge potential catchment influences. Streams spanned a gradient of landscape features and hydrological flows, with four serving as ‘potentially impacted catchment zones’ (PICZ) and …
Observing Renal Responses To Endurance Cycling In The Heat, Cody Smith
Observing Renal Responses To Endurance Cycling In The Heat, Cody Smith
Graduate Theses and Dissertations
AIM: To observe the effects of endurance cycling in the heat on renal function, and determine if the extent of impairment is related to hydration. METHODS: 40 cyclists (34 male, 6 female, 52 ± 9 y, 21.7 ± 6.5 % body fat) completed an endurance cycling event (5.7 ± 1.2 hours) in the heat (33.2 ± 5.0ºC, 38.4 ± 10.7% RH). Body mass was assessed to determine net fluid loss, muscle damage in the legs was assessed with a pain scale, a urine sample was collected to analyze hydration status, and a blood sample was drawn. All measurements were taken …
Age End-Point Effects On Performance, Carcass Measurements, And Tenderness In Goats, Kelsey Lynn Basinger
Age End-Point Effects On Performance, Carcass Measurements, And Tenderness In Goats, Kelsey Lynn Basinger
Graduate Theses and Dissertations
Goat meat is consumed all across the world, especially in developing countries. Moreover, the meat goat industry is growing in popularity in the United States due to ethnic demand of an increasing immigrant population. The objective of this study was to measure age end-point effects on performance, carcass measurements, and tenderness in goats. Intact Kiko × Boer F1 male (n = 46) kids were born on pasture in the spring of 2014 and 2015, weaned at approximately 100 d of age, and weighed monthly. Creep feed was offered to kids from birth to weaning and a high concentrate diet was …
High Throughput Phenotypic Evaluation Of Drought-Related Traits In Soybean, Hua Bai
High Throughput Phenotypic Evaluation Of Drought-Related Traits In Soybean, Hua Bai
Graduate Theses and Dissertations
Drought limits crop growth and yield in soybean. Rapid and effective methods of screening large numbers of soybean lines for drought tolerance are urgently needed. Two experiments were conducted to evaluate the effects of drought in soybean during reproductive stages. In the first experiment five genotypes from maturity groups 2 through 5 were tested under well-irrigated and drought conditions. Beginning at R5, leaf samples were taken for nitrogen concentration analysis. Pictures were taken across the top of each plot to determine the intensity of greenness using the Dark Green Color Index (DGCI). Aerial photographs were also taken to determine aerial …
Recognition Training For Faces Across Age Gaps, William Blake Erickson
Recognition Training For Faces Across Age Gaps, William Blake Erickson
Graduate Theses and Dissertations
Face recognition is a problem that has theoretical and applied value. However, the fact of facial aging is rarely addressed in research and unmentioned in the major theories of face recognition. Facial aging also has ramifications for missing persons and fugitive cases, confounding attempts by law enforcement to recover these people whose last known images are years or decades out of date. This dissertation reports three studies aimed at measuring baseline age-gap recognition ability and testing various training regimens designed to increase accuracy rates for this unique kind of recognition task.
Listeria Monocytogenes Strain Variation: Starvation, Metabolism, And Macrophage Survival, Nathan A. Jarvis
Listeria Monocytogenes Strain Variation: Starvation, Metabolism, And Macrophage Survival, Nathan A. Jarvis
Graduate Theses and Dissertations
Listeria spp. are often isolated from raw and processed food products. Listeria monocytogenes is responsible for death rates of 10 to 30% in immune compromised people; it can also infect a vast range of animals. There are numerous reports of poultry harboring or becoming infected with L. monocytogenes. First, this research assessed the ability of L. monocytogenes to replicate within chicken macrophages. Using the gentamicin-killing assay, a cell culture of chicken macrophage-like cells (HD11) were infected with two strains of L. monocytogenes individually (EGD-e and Scott A). Results show that unlike data on mouse macrophages, intracellular L. monocytogenes do not …
Genetic Determinism Of Xanthomonas Arboricola Pv. Pruni (Xap) Resistance, Fruit Quality, And Phenological Traits In Peach And Incorporation Of Marker-Assisted Selection (Mas) In The University Of Arkansas Peach And Nectarine Breeding Program, Terrence James Frett
Graduate Theses and Dissertations
For over two decades the incorporation of marker-assisted selection (MAS) has been discussed as an innovative approach to increase peach breeding efficiency. Although hundreds of quantitative trait loci (QTLs) have been identified, only a few have been converted into usable MAS tools. This highlights a disconnect between genomic discoveries and practical application in breeding programs, which has delayed use of MAS.
In this dissertation, three sequential steps were performed with the objective to bridge this gap and develop breeding-relevant DNA tests for MAS of fruit bacterial spot resistance (Xap1 and Xap6), fruit quality [blush (Rf), acidity (D), and acidity and …
Dissemination And Persistence Of Plasmid Located, Integron Associated Antibiotic Resistant Genes In Wastewater Treatment Plant Effluent And Stream Water Bacteria, _ Suhartono
Graduate Theses and Dissertations
Transmissible plasmid-mediated integrons play important role in the persistence and dissemination of antibiotic resistance throughout the environment. Plasmids from 139 multi-antibiotic resistant Escherichia coli recovered from wastewater treatment plant effluent and upstream and downstream receiving stream water in Northwest Arkansas were extracted and profiled. Genes of class 1 and class 2 integrase (intI), mobilization (mob), sulfamethoxazole resistance (sul), and trimethoprim resistance (dfr) were detected using PCR and confirmed through DNA sequencing. Plasmids from almost half of the isolates (47%) were transmissible with mobF12 gene as the most frequently detected mobilization gene. Plasmid-borne class 1 with and without class 2 integrons …
Heat Stress Effects On Cotton During Reproductive Development And Subsequent Acclimation Responses, Toby Ryan Fitzsimons
Heat Stress Effects On Cotton During Reproductive Development And Subsequent Acclimation Responses, Toby Ryan Fitzsimons
Graduate Theses and Dissertations
High temperature stress is among the most difficult to control abiotic factors affecting crop yields in the Southern United States due to its wide regional influence. Cotton (Gossypium hirsutum L.) though a tropical plant in origin, it is sensitive to the effects of high temperature. This is of particular concern when the warmest temperatures coincide with the most sensitive developmental stage of flowering. Thus, the capacity to improve cotton’s ability to tolerate heat stress has been a significant focus for many decades. Therefore, this research was composed of several different components all designed to investigate heat stress effects. Using a …
Development Of Infrared Heating Technology For Corn Drying And Decontamination To Maintain Quality And Prevent Mycotoxins, Shantae Wilson
Development Of Infrared Heating Technology For Corn Drying And Decontamination To Maintain Quality And Prevent Mycotoxins, Shantae Wilson
Graduate Theses and Dissertations
Infrared (IR) heating of corn followed by tempering treatments has potential to rapidly dry and simultaneously decontaminate corn without adverse effect on the overall quality. However, it is vital to determine the optimal processing parameters that maximize throughput and minimize drying energy without affecting overall corn quality. This study investigated the effects of IR heating and tempering treatments on moisture removal, mold load reduction, corn color change, and drying energy requirements. In addition, the study evaluated the feasibility of scaling up IR drying process using a newly built, pilot scale IR dryer. Freshly harvested corn with initial moisture content (IMC) …
Spinach (Spinacia Oleracea L.) Seed Germination And Whole Plant Growth Response To Heat Stress And Association Mapping Of Bolting, Tallness And Erectness For Use In Spinach Breeding, Jessica Chitwood
Graduate Theses and Dissertations
The effect of temperature on spinach seed germination was evaluated using a total of nine spinach genotypes and seven temperatures: 10, 15, 20, 25, 30, 32, and 35 oC in growth chambers. Genetic variation was observed. ‘Donkey’, ‘Marabu’, and ‘Raccoon’ showed higher seed germination percentage with over 70% at 30 and 32 oC, indicating the three spinach genotypes had heat-tolerance for germination. However, all spinach genotypes except ‘Ozarka II’ had lower germination percentages of less than 30% while ‘Ozarka II’ had 63% at 35 oC, indicating ‘Ozarka II’ may be a source of heat-tolerance for seed germination.
Seed germination may …
Development Of A Biofilm Model For Evaluating Poultry Drinking Water Sanitation Procedures, Pramir Maharjan
Development Of A Biofilm Model For Evaluating Poultry Drinking Water Sanitation Procedures, Pramir Maharjan
Graduate Theses and Dissertations
Enclosed water system has created a minimal sanitation focus leading to biofilm issues which are a source of flock health challenges. A series of in vitro tests were conducted to evaluate biofilm growth on polyvinylchloride (PVC) surfaces when exposed to treated/untreated water sources (test water) that are typically supplied in commercial barns. PVC test coupons (15.16 cm2) were immersed in test water in beakers to grow biofilm. Test water supplies were characterized for microbial, mineral and pH content. Temperature of test water was set at 90 °F (32.2 ᵒC) on d1 and then dropping 1°F each day over 7-day period …
The Effects Of Hypohydration On Neurocognitive, Balance, Vestibular Ocular Motor Functions And Mood State, Satoshi Iida
The Effects Of Hypohydration On Neurocognitive, Balance, Vestibular Ocular Motor Functions And Mood State, Satoshi Iida
Graduate Theses and Dissertations
Inconsistent findings have been observed on the impact of hydration state on cognitive functions. The isolated effect of hypohydration on neurocognitive performance, balance, vestibular ocular motor function and mood outcomes in widely used concussion assessment tools has not been studied. The purpose of this study was to investigate how hypohydration affected the results in concussion testing batteries. A single-blind randomized crossover design was used. Thirteen healthy males (22 ± 4y, 180.9 ± 5.7cm) without history of concussion within the past six months or any condition or disease that could influence outcome measurements participated in this study. Each subject completed concussion …
Effects Of Delayed Drying And Storage Conditions On Milling, Color, And Viscosity Properties Of Rice, Kathryn Nicole Haydon
Effects Of Delayed Drying And Storage Conditions On Milling, Color, And Viscosity Properties Of Rice, Kathryn Nicole Haydon
Graduate Theses and Dissertations
Head rice yields (HRYs), kernel discoloration, and functional properties are three hallmarks of rice quality, and can be impacted after harvest by storage conditions. This study evaluated impacts on rice quality due to controlled storage at moisture contents (MCs), temperatures, and storage durations that may be incurred during Mid-South rice farming practices. Three hybrid, long-grain cultivars, harvested in Arkansas in 2014 and 2015, were stored in rough rice form at 4 MCs (12.5%, 16%, 19%, and 21%), and 5 temperatures (10°C, 15°C, 20°C, 27°C, and 40°C), for 16 weeks, with samples taken every 2 weeks. After drying and milling, HRYs, …
Use Of A Blunt Version Of Meullenet-Owens Razor Shear To Analyze Meat Qualities Of Broilers With Woody Breast Myopathy And Reared To Various Ages, Famous Lue Yang
Use Of A Blunt Version Of Meullenet-Owens Razor Shear To Analyze Meat Qualities Of Broilers With Woody Breast Myopathy And Reared To Various Ages, Famous Lue Yang
Graduate Theses and Dissertations
Broiler breast meat tenderness is an important meat quality attribute. There has been an increase in incidences of woody breast along with white striping as meat quality defects. These defects have been found to negatively impact meat qualities of broiler breast fillets. Woody breast can be classified by the degree of hardness into normal (NORM) and severe (SEV). The Meullenet-Owens Razor Shear (MORS) method was developed to assess broiler meat tenderness and a blunt version (BMORS) has been reported to be more sensitive at higher degrees of toughness. The present study was intended to determine the effect of age, debone …