Life Sciences Commons^™

Assessment Of Novel Protein Ingredient Arthrospira Platensis (Microalgae) And Soybean Genotype Amino Acid And Oil Selection Improvements On Broiler Performance, Savannah Wells Crafton

Graduate Theses and Dissertations

Two experiments were conducted to assess the efficiency of including novel protein ingredient Arthrospira platensis or improved soybean meal in a broiler diet. The first experiment aimed to determine the feeding value of soybean meal produced from varieties of soybeans bred for increased amino acid content (SBAA) improved oil content (SBO) compared to a conventional soybean variety in an ANOVA design fed to Cobb 500 female broilers for 28-42d of age. The SBAA and SBO soybeans contained overall higher amino acid content and lower oligosaccharide content compared to the conventional soybean variety in addition to improved oil quality. The second …

Identification Of Genomic, Proteomic, And Metabolomic Signatures Associated With Pulmonary Hypertension Syndrome In Broilers, Duaa Almansaf

Graduate Theses and Dissertations

The present dissertation contains a collection of studies that examine the genomic, proteomic, and metabolomic association to pulmonary hypertension or ascites phenotype in fast-growing broilers. Pulmonary hypertension is a multifactorial metabolic disease influenced by physiological, environmental, and nutritional factors. It is characterized by a number of structural changes including, thrombosis and adverse pulmonary vascular remodeling. Thus, the atrial pressure is increased, and the right ventricle becomes hypertrophied, resulting in heart failure and the death of the bird. Pulmonary hypertension or ascites is a global problem that has negatively impacted the economy. The increased mortality rate of broilers (25%) is estimated …

Evaluation Of The Effect Of A Lippia Organoides Essential Oil Extract On Clostridium Perfringens Proliferation In Vitro And Necrotic Enteritis In Broiler Chickens, Makenly E. Coles

Graduate Theses and Dissertations

The purpose of the present research was to assess the effects of essential oils derived from the plant Lippia organoides on performance parameters, intestinal integrity, and necrotic enteritis (NE) in broiler chickens. To do this, a previously established challenge model for NE was utilized which included challenging with Salmonella Typhimurium on day 0, Eimeria maxima on day 18, and Clostridium perfringens on days 22 and 23. Treatment groups included a 1) non-challenged, negative control, 2) challenged control, and 3) challenged, Lippia origanoides (37ppm in the diet). Group 1 (negative control) had significantly (P < 0.05) higher body weight gain from d8-25 and d0-25 compared to both challenged groups. Feed intake was significantly different for all three groups (P < 0.05) at 8-25 days and 0-25 days with group 1 having the highest feed intake for both time periods. Total mortality was greater in the positive control when compared to both the negative and treatment groups. NE lesion scores were significantly different between all groups with the positive control having the highest mean lesion scores and the negative control having a mean lesion score of 0. The positive control group had the highest FITC-d amounts detected in the sera, being statistically higher than both the treatment group and negative group which were both statistically different from each other. At the present inclusion rate for the essential oil (37 ppm), there was an overall reduction in the negative impact from the NE infection. Further studies should be conducted to reach more significant conclusions.

Divergent Selection For Water Conversion Ratio In Broiler Populations, Joseph Hiltz

Graduate Theses and Dissertations

Water scarcity is a global reality and with the anticipated population growth, freshwater resources will be further strained to meet both human needs and agriculture applications. To ensure a water sustainable and food secure future, all aspects of agriculture must become more efficient. Two strategies were explored. The potential of improving water efficiency in broilers was examined. The first strategy was to develop a more efficient and accurate method for measuring water consumption/inputs in agriculture. To date, water measuring technology has lacked the necessary sophistication to assure accuracy and repeatability of low flow water usage.

After establishing a low flow …

Distillers Dried Grains With Solubles: Determination Of Metabolizable Energy Values Using Regression-Based Assays And Their Correlation With Chemical Composition, Kenia Mitre

Graduate Theses and Dissertations

The inherent variability and lack of standardization of in vivo assays to determine the metabolizable energy (ME) of DDGS has led to inconsistent values and limited its potential to use in broiler diets. Therefore, two experiments were conducted to characterize the content of nitrogen-corrected ME (MEn) in 11 DDGS sources obtained from various ethanol plants using a regression-based broiler assay and to attempt to develop equations to predict MEn based on its chemical composition. In experiment 1, the objectives were to determine the influence of inclusion level and feed intake on the resulting ME and MEn values of a single …

Effects Of Dietary Copper, Zinc And Manganese Source And Level On The Acute Inflammatory Response Of Broilers, Chelsea Ellington

Graduate Theses and Dissertations

Trace minerals, particularly copper, zinc and manganese, play a role in ensuring optimal immune function. To examine the effects of diets containing different levels and sources of trace minerals on the inflammatory response, Cobb 500 broilers were fed four experimental diets containing 5 mg/kg Cu and 45 mg/kg of Zn and Mn or 10 mg/kg Cu and 90 mg/kg Zn and Mn in either the sulfate or hydroxychloride form. When the broilers were 5 weeks of age, 16 μg of lipopolysaccharide (LPS) was injected into the pulp of growing feathers (GF; 16 GF per chicken; 1 μg LPS per GF). …

Investigating Growth Performance And Intestinal Barrier Integrity In Heat-Stressed Modern Broilers And Their Ancestor Jungle Fowl, Travis Tabler

Graduate Theses and Dissertations

Heat stress (HS) has a negative effect on poultry production sustainability due to its adverse consequence on bird welfare, health, growth, and mortality. Although modern broilers have greater gut mass and higher energy use efficiency than unselected birds, they are more vulnerable to HS that induces “leaky gut syndrome,” or increased intestinal permeability. The aim of the current study was to determine the effect of HS on growth performance and gut barrier integrity in three modern broiler lines and their ancestor the Jungle Fowl. Four chicken populations including Giant Jungle Fowl (JF), Athens Canadian Random Bred (ACRB), 1995 Arkansas Random …

Genome Wide Association And Next Generation Sequencing Approaches To Map Determinants Of Ascites In Broiler Chickens, Shatovisha Dey

Graduate Theses and Dissertations

These studies have investigated different candidate genomic regions for their contributions to ascites in broilers. Ascites syndrome is a manifestation of idiopathic pulmonary arteriole hypertension that concerns the poultry industry worldwide. Investigations have demonstrated the disease to be genetically regulated and to exhibit moderate to high heritabilities. Although previous studies have indicated a few chromosomes to be involved with ascites, no genes have been identified to date with direct links to the disease. This dissertation presents a collection of studies that determine the genomic and genetic interactions for regions on chromosome 2 and 9 for ascites phenotypes in broiler chickens. …

Effect Of Light Intensity On Production Parameters And Feeding Behavior Of Broilers, Maurice Raccoursier Frost

Graduate Theses and Dissertations

This project was performed in two parts. The first was focused on light intensity as it affects performance. A randomized complete block design (RCBD) was performed. Broilers, Cobb 500 (n = 1584) were housed in 3 commercial houses (121.9 x 12.2 m). In each house birds were randomized and placed in 72 pens of 121.9 x 121.9 cm (22 bird/pen, males and females). All the treatment groups were provided with 24h light (L) during the first week and then 18L:6Dark (D) and 20 lux from day 7 to 14. The 3 intensity treatments of 5 lux (lx), 10 lx and …

Characterization Of Broiler Lines Divergently Selected For Breast Muscle Color, Sara Katherine Orlowski

Graduate Theses and Dissertations

An increase in the consumption of poultry has generated an increase in demand for higher yielding broilers. This has led to an increase in atypical meat and issues with appearance. Color is a direct result of a pH decline as meat goes through rigor mortis with meat generally becoming lighter. If the pH declines too rapidly or too slowly, meat quality can suffer. Physical properties of meat can be altered by pH. A fast pH decline results in pale meat with decreased tenderness. A slow pH decline can result in darker meat with a reduced shelf-life. With a known relationship …

Evaluation Of Bacillus Spp Candidates For Aflatoxin B1 Biodegradation In Broiler Chickens, Rosario Galarza Seeber

Graduate Theses and Dissertations

Poultry health has been traditionally maintained by hygenic measures, vaccinations, and the use of antibiotics. Modern husbandry management considers the use of probiotics as a natural way to protect birds against many everyday pathogens. Different strains of Bacillus spp. have proved to have beneficial effects in poultry production. However, the most used bacterium in commercial probiotics is Lactobacillus, a vegetative cell. In contrast, Bacillus spp are bacterial spores, highly resistant to harsh conditions, which makes them preferable, in some cases, to Lactobacillus because of shelf life and storage conditions. There is published information regarding mycotoxin detoxification by bacteria. Mycotoxins are …

Calorimetry And Body Composition Research In Broilers And Broiler Breeders, Justina Victoria Caldas Cueva

Graduate Theses and Dissertations

Indirect calorimetry to study heat production (HP) and dual energy X-ray absorptiometry (DEXA) for body composition (BC) are powerful techniques to study the dynamics of energy and protein utilization in poultry. The first two chapters present the BC (dry matter, lean, protein, and fat, bone mineral, calcium and phosphorus) of modern broilers from 1 – 60 d of age analyzed by chemical analysis and DEXA. DEXA has been validated for precision, standardized for position, and equations and validations developed for chickens under two different feeding levels. These equations are unique to the machine and software in use. Research in broilers …

Intestinal Inflammation Model Inducing Gut Leakage By Different Methods In Broiler Chickens Using Fitc-D As A Marker, Eduardo Vicuna

Graduate Theses and Dissertations

Traditionally, antibiotic growth promoters (AGP) have been used in food animals to reduce enteric inflammation and maintain intestinal homeostasis, thus improving performance. Due to increasing restrictions regarding the use of AGP, precise and high throughput enteric inflammation models and markers to search for effective alternatives are urgently needed. Oral administration of fluorescein isothiocyanate dextran (FITC-d) and its passage into blood can be used as a marker for tight junction permeability. FITC-d is a large molecule (3-5 kDa) which does not usually leak through the intact gastrointestinal tract barrier. However, when conditions disrupt the tight junctions between epithelial cells, the FITC-d …

Evaluation Of Phytate And Phytase Interactions And Phytase Phase-Feeding On Bird Performance, Bone Characteristics And Meat Quality In Young Growing Broilers, Chance L. Williams

Graduate Theses and Dissertations

Two trials were performed using one-day-old male Cobb x Cobb 500 broilers to determine how dietary phytate and phytase levels as well as phytase phase feeding impacted bird performance parameters, tibia characteristics, and malonaldehyde (MDA) content of the liver, breast and thigh tissues. The first experiment consisted of 1,008 birds randomly placed in 48 floor pens within two commercial broiler houses at the Applied Broiler Research Farm (ABRF; 21 birds per pen; 0.76 ft2 per bird). A 2 X 3 factorial design was used with two levels of dietary phytate (0.21 and 0.31 %) and three levels of phytase supplementation …

Correlated Response To Selection And Effects Of Pre-Slaughter Environment On Meat Quality In Broilers Divergently Selected For Muscle Color, Isaac David Harford

Graduate Theses and Dissertations

Two broiler lines divergently selected for high (HMC) and low (LMC) muscle lightness (L*) were formed from a random bred control line (RBC). After eight generations of selection, three experimental trials were conducted to investigate the correlated response to selection and effects of pre-slaughter environment on meat quality in all three lines.

In the first experiment, a total of 100 birds from each line were bled for blood chemistry analysis and were evaluated for meat quality the following day. The HMC line had higher fillet drip loss (DL), L*, heterophil to lymphocyte ratio (H:L), and platelet count (Plt), and lower …

Chitosan Reduces Enteric Colonization Of Campylobacter In Young Chickens, But Not On Post-Harvest Chicken Skin Samples, Hanna Royce Arambel

Graduate Theses and Dissertations

Worldwide, Campylobacter is one of the leading causes of foodborne bacterial gastroenteritis causing an estimated 1.3 million infections in the United States alone. Consumption and/or cross-contamination of raw or undercooked poultry products have been linked as the most common source of Campylobacter infection, making the poultry industry a target for Campylobacter reduction strategies. Campylobacter is prevalent in most poultry flocks in the United States, with as many as 90% of flocks Campylobacter-positive at the time of slaughter. It is estimated that a reduction of Campylobacter in poultry would greatly reduce the risk of campylobacteriosis in humans. Unfortunately, there are …

Molecular Diagnosis Of Metabolic Fast Growth Related Diseases In Broiler, Adnan Ali Khalaf Al-Rubaye

Graduate Theses and Dissertations

Pulmonary Hypertension Syndrome (PHS) and lameness are important metabolic diseases that affect rapidly growing broilers. The research reported in the first section of this dissertation focused on developing qPCR assays to identify differences in the expression levels of four candidate genes possibly associated with PHS: angiotensin II type 1 receptor (AGTR1): urotensin receptor 2D (UTS2D); serotonin receptor/transporter type 2Bn (HTR2B); and angiotensinogen cleaving enzyme (ACE). Expression levels of these candidate genes were examined in four different tissues. We established ribosomal protein S14 (RPS14) and RNA polymerase subunit 2B (RP2B) as suitable reference genes because they showed the most consistent deltaCt …

Response Of Broiler Chickens To Variation In Dietary Nutrient Content, Sarah Diane Goodgame

Graduate Theses and Dissertations

Rising feed costs, environmental pollution concerns targeted at animal agriculture, a worldwide focus on sustainability and the never ending battle to improve production and efficiency within the poultry industry challenges nutritionists to focus on products to improve nutrient digestibility and decrease feed costs while at the same time maximizing performance. The research presented in this dissertation focuses on improving nutrient utilization of protein sources with protein liberating enzymes currently available commercially. The research also conducted addresses the addition of crystalline amino acids valine and isoleucine and their effect on improving efficiency of broilers.

The first research project compared the efficacy …

Comparative Response Of Different Broiler Genotypes To Dietary Nutrient Levels, Franco Javier Mussini

Graduate Theses and Dissertations

ABSTRACT

Three studies were conducted to evaluate how the broiler has changed over the years due to intense genetic selection. Four different broiler genotypes, one unselected since the 1950's (HER) representing the old meat-type bird and three current strains (Ross 308, Ross 708, and a test product (TY)) were studied. Differences in growth, morphometry, and response in performance and processing yield to different nutrient levels were studied.

Experiment 1 evaluated growth and morphometric analysis. Birds of each strain were killed weekly from day 7 to day 56, necropsied and cut up parts and organs weighed. Measurement of small intestine segments …

The Incidence Of Plexiform Lesion Formation In Lines Divergently Selected For Ascites, James Grant Mason

Graduate Theses and Dissertations

Complex Vascular Lesions, (CVL) are a common vascular change associated with idiopathicpulmonary arterial hypertension (IPAH). CVL have been characterized in studies using animal models involving, but not limited to, Sprague-Dawley rats and poultry (Abe et al., 2010; Wideman et al., 2011).IPAH has been documented in poultry although no connection has been made with CVLs. The current study characterizes CVL in the lungs from broilers derived from lines divergently selected for 15 generations for ascites susceptibility under conditions of simulated high altitude. The ascites RES and SUS lines were sampled for CVL incidence over time. Both lines were reared in a …