Life Sciences Commons^™

Broad-Spectrum Lignin-Based Adhesives Using Thiol–Silyl Ether Crosslinkers, Yishayah Bension, Siteng Zhang, Tristan Menninger, Ting Ge, Chuanbing Tang

Faculty Publications

Lignin is a renewable feedstock that is abundant and inexpensive but still presents challenges for its valorization. In this work, we converted functionalized lignin into broad-spectrum adhesives using thiol–silyl ether crosslinkers. The curing behavior of adhesives was investigated via rheology of their resin forms. These materials exhibit good adhesion on diverse substrates, including wood, glass, steel, aluminium, carbon fiber, and different plastics, with the most adhesion strength in the range of 1–3 MPa. These adhesives were also explored for applications, ranging from wet conditions to different mechanically responsive materials. The mechanism of adhesion was further examined to understand the bonding …

New Genera And Species Of Caulobacter And Brevundimonas Bacteriophages Provide Insights Into Phage Genome Evolution, Bert Ely, Michael Hils, Aaron Clarke, Maegan Albert, Nadia Holness, Jacob Lenski, Tannaz Mohammadi

Faculty Publications

Previous studies have identified diverse bacteriophages that infect Caulobacter vibrioides strain CB15 ranging from small RNA phages to four genera of jumbo phages. In this study, we focus on 20 bacteriophages whose genomes range from 40 to 60 kb in length. Genome comparisons indicated that these diverse phages represent six Caulobacter phage genera and one additional genus that includes both Caulobacter and Brevundimonas phages. Within species, comparisons revealed that both single base changes and inserted or deleted genetic material cause the genomes of closely related phages to diverge. Among genera, the basic gene order and the orientation of key genes …

Did God Create Viruses?, James Hayward

Faculty Publications

No abstract provided.

Comparison Of Raw Accelerometry Data From Actigraph, Apple Watch, Garmin, And Fitbit Using A Mechanical Shaker Table, James W. White Iii, Olivia L. Finnegan, Nick Tindall, Srihari Nelakuditi, David E. Brown Iii, Russell R. Pate Ph.D., Gregory J. Welk, Massimiliano De Zambotti, Rahul Ghosal, Yuan Wang, Sarah Burkart, Elizabeth L. Adams, Mvs Chandrashekhar, Bridget Armstrong, Michael W. Beets, Robert Weaver Med, Phd

Faculty Publications

The purpose of this study was to evaluate the reliability and validity of the raw accelerometry output from research-grade and consumer wearable devices compared to accelerations produced by a mechanical shaker table. Raw accelerometry data from a total of 40 devices (i.e., n = 10 ActiGraph wGT3X-BT, n = 10 Apple Watch Series 7, n = 10 Garmin Vivoactive 4S, and n = 10 Fitbit Sense) were compared to reference accelerations produced by an orbital shaker table at speeds ranging from 0.6 Hz (4.4 milligravity-mg) to 3.2 Hz (124.7mg). Two-way random effects absolute intraclass correlation coefficients (ICC) tested inter-device reliability. …

Balancing Best Practice And Reality In Behavioral Intervention Development: A Survey Of Principal Investigators Funded By The National Institutes Of Health, Lauren Von Klinggraeff, Sarah Burkart, Christopher D. Pfledderer, Alexander Mclain, Bridget Armstrong Ph.D., Robert Glenn Weaver Med, Phd, Michael Beets Med, Mph, Phd

Faculty Publications

Preliminary studies play a prominent role in the development of large-scale behavioral interventions. Though recommendations exist to guide the execution and interpretation of preliminary studies, these assume optimal scenarios which may clash with realities faced by researchers. The purpose of this study was to explore how principal investigators (PIs) balance expectations when conducting preliminary studies. We surveyed PIs funded by the National Institutes of Health to conduct preliminary behavioral interventions between 2000 and 2020. Four hundred thirty-one PIs (19% response rate) completed the survey (November 2021 to January 2022, 72% female, mean 21 years post-terminal degree). Most PIs were aware …

Whole-Of-School Physical Activity Implementation In The Context Of The Dubai Fitness Challenge, Chris Mcmahon, Collin A. Webster, Robert Weaver, Christophe El Haber, Gönül Tekkurşun Demir, Zainab Mohamed Ismail, Syeda Zoha Fatima Naqvi, Mehnaz Ghani, Şevval Kepenek, Manel Kherraf, Thrisha Krishnakumar, Pranati Prakash, Yeowon Seo

Faculty Publications

Introduction

Physical activity (PA) promotion among school-aged youth is a global health priority. Recommendations for such promotion include implementing whole-of-school approaches that maximize resources across the school environment. This study examined schools’ participation in an annual, government-led, and emirate-wide initiative in Dubai, called the Dubai Fitness Challenge, in which the goal is to accrue 30 minutes of PA every day for 30 days (as such, the initiative is colloquially referred to as “Dubai 30x30”).

Methods

A mixed-methods design was employed for this study. Three schools were recruited using convenience sampling. Participants were 18 physical education teachers, 20 classroom teachers, 2 …

Extending Irrigation Reservoir Histories For Improved Groundwater Modeling And Conjunctive Water Management In Two Arkansas Critical Groundwater Areas, Daniel D. Shults, Michele L. Reba, John W. Nowlin, Joseph Massey, Quentin Read

Faculty Publications

Agricultural land managers in the Mississippi Alluvial Plain rely on the Mississippi River Valley Alluvial Aquifer for the majority of the water used for irrigation in the region. Pumping that outpaces recharge has resulted in significant cones of depression in the region, increasing pumping costs and forcing producers to use more surface water, often in the form of constructing on-farm storage reservoirs. At the same time, efforts are underway to improve groundwater models to run scenarios of conjunctive water management in the region. The improved models require accurate depiction of how the system is being managed including the use of …

Unimer Suppression Enables Supersaturated Homopolymer Swollen Micelles With Long-Term Stability After Glassy Entrapment, Eric R. Williams, Christian X. Ruff, Morgan Stefik

Faculty Publications

Micelle sizes are critical for a range of applications where the simple ability to adjust and lock in specific stable sizes has remained largely elusive. While micelle swelling agents are well-known, their dynamic re-equilibration in solution implies limited stability. Here, a non-equilibrium processing sequence is studied where supersaturated homopolymer swelling is combined with glassy-core (“persistent”) micelles. This path-dependent process was found to sensitively depend on unimer concentration as revealed by DLS, SAXS, and TEM analysis. Here, lower-selectivity solvent combinations led to the formation of unimer-homopolymer aggregates and eventual precipitation, reminiscent of anomalous micellization. In contrast, higher-selectivity solvents enabled supersaturated homopolymer …

Unimer Suppression Enables Supersaturated Homopolymer Swollen Micelles With Long-Term Stability After Glassy Entrapment, Eric R. Williams, Christian X. Ruff, Morgan Stefik

Faculty Publications

Micelle sizes are critical for a range of applications where the simple ability to adjust and lock in specific stable sizes has remained largely elusive. While micelle swelling agents are well-known, their dynamic re-equilibration in solution implies limited stability. Here, a non-equilibrium processing sequence is studied where supersaturated homopolymer swelling is combined with glassy-core (“persistent”) micelles. This path-dependent process was found to sensitively depend on unimer concentration as revealed by DLS, SAXS, and TEM analysis. Here, lower-selectivity solvent combinations led to the formation of unimer-homopolymer aggregates and eventual precipitation, reminiscent of anomalous micellization. In contrast, higher-selectivity solvents enabled supersaturated homopolymer …

Left Ventricle Function And Post-Transcriptional Events With Exercise Training In Pigs, Stephanie L. Samani, Shayne C. Barlow, Lisa A. Freeburg, Traci L. Jones, Marlee Poole, Mark A. Sarzynski Ph.D., Faha, Facsm, Michael R. Zile, Tarek Shazly, Francis G. Spinale

Faculty Publications

Background

Standardized exercise protocols have been shown to improve overall cardiovascular fitness, but direct effects on left ventricular (LV) function, particularly diastolic function and relation to post-transcriptional molecular pathways (microRNAs (miRs)) are poorly understood. This project tested the central hypothesis that adaptive LV remodeling resulting from a large animal exercise training protocol, would be directly associated with specific miRs responsible for regulating pathways relevant to LV myocardial stiffness and geometry.

Methods and results

Pigs (n = 9; 25 Kg) underwent a 4 week exercise training protocol (10 degrees elevation, 2.5 mph, 10 min, 5 days/week) whereby LV chamber stiffness (KC) …

Ras/Mapk Signaling Mediates Adipose Tissue Control Of Ovarian Germline Survival And Ovulation In Drosophila Melanogaster, Tancia Bradshaw, Chad Simmons, Rachael Ott, Alissa Richmond Armstrong

Faculty Publications

From insects to humans, oogenesis is tightly linked to nutritional input, yet little is known about how whole organism physiology matches dietary changes with oocyte development. Considering that diet-induced adipose tissue dysfunction is associated with an increased risk for fertility problems, and other obesity-associated pathophysiologies, it is critical to decipher the cellular and molecular mechanisms linking adipose nutrient sensing to remote control of the ovary and other tissues. Our previous studies in Drosophila melanogaster have shown that amino acid sensing, via the amino acid response pathway and mTOR-mediated signaling function within adipocytes to control germline stem cell maintenance and ovulation, …

The Cetacean Sanctuary: A Sea Of Unknowns, Jason N. Bruck

Faculty Publications

Housing cetaceans in netted sea pens is not new and is common for many accredited managed- care facilities. Hence, the distinction between sanctuary and sea pen is more about the philosophies of those who run these sanctuary facilities, the effects of these philosophies on the animals’ welfare, and how proponents of these sanctuaries fund the care of these animals. Here, I consider what plans exist for cetacean sanctuaries and discuss the caveats and challenges associated with this form of activist-managed captivity. One goal for stakeholders should be to disregard the emotional connotations of the word “sanctuary” …

An Empirical Investigation Of Online Grocery Shopping Behaviors Based On Different Generations, Jung-Im Seo

Faculty Publications

The Coronavirus 19 (COVID-19) pandemic has significantly impacted on consumers’ grocery shopping habits and lifestyles. Online grocery shopping is one of the promising ways to have little contact as well as increase convenience. For this reason, the sales and revenues of online grocery shopping have been dramatically increasing during the pandemic. The purpose of this study to explore online grocery shopping behaviors of different generations and their shopping intentions toward online grocery shopping during and after the pandemic. Data were collected from Amazon Mechanical Turk (MTurk) and Southeast region in U.S.A with convenience sample. All Generations know that online grocery …

Ecological Diversification In An Adaptive Radiation Of Plants: The Role Of De Novo Mutation And Introgression, Benjamin Stone, Carolyn A. Wessinger

Faculty Publications

Adaptive radiations are characterized by rapid ecological diversification and speciation events, leading to fuzzy species boundaries between ecologically differentiated species. Adaptive radiations are therefore key systems for understanding how species are formed and maintained, including the role of de novo mutations versus preexisting variation in ecological adaptation and the genome-wide consequences of hybridization events. For example, adaptive introgression, where beneficial alleles are transferred between lineages through hybridization, may fuel diversification in adaptive radiations and facilitate adaptation to new environments. In this study, we employed whole-genome resequencing data to investigate the evolutionary origin of hummingbird-pollinated flowers and to characterize genome-wide patterns …

Analyzing Water Resource Challenges In The Us Eastern Zone, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

One major narrative over the last several years, consistent with the US Northeast region in policy discourse rests on its perception as a hub with high concentration of city bright lights and skyscrapers but short on water usage. In the process, no one thinks about how the rising demands from urban residential areas to hydroelectricity and climate change stressors impact the hydrology. Yet, what is forgotten is that the economic engine driving the region comes from the interactions in a complex hydrological network anchored in water distribution dependency. At the same time, a closer look at various states indicates that …

Evaluating Climate Change Hazards Within The Us Midwest Zone, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

The US Midwest region known for its web of lakes, rivers and vast swaths of farmland ranks high as the nation’s food basket. Being a huge agricultural hub tied to global markets, the region’s immediate influence spans across multiplicity of states supported by a flourishing supply chain network sustained by farming centers and activities in and outside of the Midwest. Notwithstanding the region’s place as an active farm hub, it is slowly emerging as an epicenter of changing climatic hazards with the manifestations evident in different forms over the years. With many of the risks and recurrent changes now a …

The Effects Of Changing Climate In The Us Western Region, Yaw Twumasi, E. C. Merem, Y. A. Twumasi, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

he Pacific Northwest region of the US has over the years, outpaced other zones in the country in every socio-economic indicator. Known for its enviable physical attributes of high montane ecosystem and warm weather all year round in some places together with abundant biodiversity. The region’s position as among the fastest growing areas in the nation under vast swaths of land with large population concentration in big cities has made it the perennial destination for many. Aside from all these, in the last several years, the zone continues to be the epicenter of the most dangerous natural disasters prompted by …

The Analysis Of Extreme Climate Threats In Maryland’S Lower Region Along The Us Mid Atlantic, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

The state of Maryland for all intents and purposes stands out as a pacesetter in different aspects of environmental planning more than other states. Known for having the first established planning commission in the United States decades ago before its neighbors and a longstanding tradition in orderly planning. One would think such illustrious trajectory, that Maryland enjoys, guarantees immunity from common exposures to climate change dangers based on experience. However, that is not really the case in the Mid Atlantic zone. The same state that boasts of some of the most successful farming operations and a flourishing economy heavily reliant …

Assessing Climate Change Dangers Within The South Florida Region Of Us Southeast, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

In the last several decades, the southern portion of Florida continues to be an attraction, and destination for tourism given its warm environment. Aside from the benefits that accrues the region, with that have come many challenges rooted in climatic uncertainty. To that effect, it is inconceivable to venture into climate change risks analysis overtime, without cognizance of the scale and responses in Florida as a mitigation template considering the frequency therein. In the process, no matter what, the Southern Florida zone remains an epicenter of most natural disasters in the Southeast region of the US based on location and …

Analyzing The Capacities Of Cotton Production In Sudan, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

In the political economy of the nation of Sudan, cotton ranks significantly high as an essential component of the revenue generation sources. Considering the significance of the crop in the country, cotton has not only been exported to various countries, but some portions of the produce are also used up internally. With that also came, large scale cultivation of the cotton produce on vast swaths of land over the years amidst demands and fluctuations in the marketplace. Given the effects of cotton production on the environment from the use of chemicals and widespread water use, there came changes in the …

Investigating The Ecological Effects Of Agricultural Assistance Schemes In California’S Central Valley Area, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

Amidst ongoing changes in the farm sector, the disbursement of farm aid, continues to improve the welfare of growers involved in agricultural activities within the South-Central region of California. Aside from its role as a vital tool meant to maintain the delivery of government assisted farm programming directed at the needy. In the last several decades in the country, the disbursement of federal agricultural assisted programs has risen enormously in line with commitments geared at accelerating the productive capacity of farms considered eligible. With the financial packages earmarked for agri-business tied to few commodities (cotton, corn, rice, wheat), as well …

Exploring Shifts In Yam Production Trends Along Nigeria’S Southern Region, Yaw Twumasi, E. C. Merem, S. Fageir, D. Olagbegi, J. Wesley, R. Coney, Y. Babalola, T. Thomas, A. Hines, G. Hirse, G. S. Ochai, E. Nwagboso, M. Crisler, S. Leggett, J. Offiah, S. Emeakpor

Faculty Publications

Yam as a staple commodity in Nigeria, remains generally grown in different places within the ecological zones and food hubs in the nation. Notwithstanding all that, being integral part of the nation’s food security in a setting where Nigeria ranks high as the largest producer globally. Still, yam continues to find ample use with essential role for numerous purposes. Classified as an important food crop, the use of yam runs deeper, given its high demands for religious, socio-cultural practices, the economy, industries, and the marketplace. Even at that and considering the vast presence of yams over large swaths of land …

Physical Activity, Anxiety, Depression, And Coping In Turkish Men And Women During The First Wave Of Covid-19, Bijen Filiz, Funda Coşkun Özyol, Bengü Güven, Ezel Nur Korur, Yılmaz Yüksel, Cavit Işık Yavuz, Kele Ding, Jingzhen Yang, J. Larry Durstine Phd, Facsm, Faacvpr, Fnak, Ming-Kai Chin, Gıyasettin Demirhan

Faculty Publications

A need exists to better understand the relationships between COVID-19, coping behaviors, physical activity and stress, and COVID-19’s impact on way of life. A cross-sectional study design was used to examine adult physical activity, hope, depression, anxiety, and coping status by gender during the COVID-19 pandemic, and to determine the impact of these variables on the coping process. The study also examined the effect of gender on the relation between physical activity and dependent variables. A global survey instrument was used in this study, including 1,400 Turkish adults. This study identified significant gender-based differences regarding physical activity, hope, depression, anxiety, …

How Individuals’ Opinions Influence Society’S Resistance To Epidemics: An Agent-Based Model Approach, Geonsik Yu, Michael J. Garee, Mario Ventresca, Yuehwern Yih

Faculty Publications

Background: Protecting public health from infectious diseases often relies on the cooperation of citizens, especially when self-care interventions are the only viable tools for disease mitigation. Accordingly, social aspects related to public opinion have been studied in the context of the recent COVID-19 pandemic. However, a comprehensive understanding of the effects of opinion-related factors on disease spread still requires further exploration. Methods: We propose an agent-based simulation framework incorporating opinion dynamics within an epidemic model based on the assumption that mass media channels play a leading role in opinion dynamics. The model simulates how opinions about preventive interventions change over …

The Effects Of A Brand-Specific, Hemp-Derived Cannabidiol Product On Physiological, Biochemical, And Psychometric Outcomes In Healthy Adults: A Double-Blind, Randomized Clinical Trial, Gianna F. Mastrofini, Bridget A. Mcfadden, Alexa J. Chandler Ph.D., Blaine S. Lints, Harry P. Cintineo, Nathaniel D. Rhoades, Caroline S. Vincenty, Sten O. Stray-Gundersen Ph.D., Abbi D. Lane, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

Background: Cannabidiol (CBD) is a non-psychoactive phyto-cannabinoid derived from the Cannabis sativa plant. CBD exhibits various interactions at receptor sites, prompting the research of its potential anti-inflammatory, immunomodulatory, psychological, and pain-relieving effects. This study aimed to investigate the physiological, biochemical, and psychometric effects of a brand-specific, hemp-derived CBD product in healthy adults over a 12-week observation period. Methods: 54 healthy males and females (age = 25 ± 7y; BMI = 24.82 ± 3.25 kg/m2) recruited from a large Southeastern University completed the study. Participants arrived at the laboratory after > 8 h of fasting, and > 48 h without alcohol consumption and …

Common Questions And Misconceptions About Caffeine Supplementation: What Does The Scientific Evidence Really Show?, Jose Antonio, Daniel E. Newmire, Jeffrey R. Stout, Brandi Antonio, Maureen Gibbons, Lonnie M. Lowery, Joseph Harper, Darryn Willoughby, Cassandra Evans, Dawn Anderson, Erica Goldstein, Jose Rojas, Matías Monsalves-Álvarez, Scott C. Forbes, Jose Gomez Lopez, Tim Ziegenfuss, Blake D. Moulding, Darren Candow, Michael Sagner, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

Caffeine is a popular ergogenic aid that has a plethora of evidence highlighting its positive effects. A Google Scholar search using the keywords “caffeine” and “exercise” yields over 200,000 results, emphasizing the extensive research on this topic. However, despite the vast amount of available data, it is intriguing that uncertainties persist regarding the effectiveness and safety of caffeine. These include but are not limited to: 1. Does caffeine dehydrate you at rest? 2. Does caffeine dehydrate you during exercise? 3. Does caffeine promote the loss of body fat? 4. Does habitual caffeine consumption influence the performance response to acute caffeine …

Spatiotemporal Patterns For Agroforestry Tree Crops In The U.S. Corn Belt For Usda Census Of Agriculture Periods 2012–2022, Andria Caruthers, Justin Dijak, Robin M. Rotman

Faculty Publications

Within the U.S., there is a growing need to integrate environmentally friendly practices into conventional agriculture. Agroforestry enhances environmental and resource stewardship in agricultural landscapes while offering potential economic benefits to farmers. Despite rising interest, limited information on its application in the U.S. hinders development efforts. A spatiotemporal analysis of current farm operations can provide crucial insights. This study examined patterns of agroforestry and tree crop adoption in the U.S. Corn Belt using USDA Census data (2012, 2017, and 2022) and spatial tools (Global Moran’s I, Local Moran’s I, and Moran scatterplots). The tree crops included in the analysis were …

Iron–Sulfur Cluster Assembly Scaffold Protein Iscu Is Required For Activation Of Ferric Uptake Regulator (Fur) In Escherichia Coli, Huangen Ding

Faculty Publications

No abstract provided.

Density And Abundance Estimation Of West Indian Manatee, Trichechus Manatus, Between The States Of Ceará And Piauí, Northeast Brazil, Using Active Acoustics, Katherine Fiedler Choi Lima, Denis Moledo De Sousa Abessa, Daniel Gonzalez-Socoloske, Renata Sousa-Lima, Ana Carolina Oliveira De Meirelles, Cristine Pereira Negrão Silva

Faculty Publications

The West Indian manatee is one of the most threatened species in Brazil. The species has currently a patchy distribution from the state of Alagoas to the state of Amapá. The difficulty of observing manatees, especially in estuarine waters, is a challenge for conservation. Therefore, it is necessary to use new methodologies and technologies to solve manatee detection problems in their natural habitats. The goal of this study was to use an active acoustic method of detection to estimate manatee density and abundance in the estuarine complex of the Timonha and Ubatuba rivers, between the states of Ceará and Piauí, …

International Society Of Sports Nutrition Position Stand: Long-Chain Omega-3 Polyunsaturated Fatty Acids, Alex Leaf, Jeffrey A. Rothschild, Tim M. Sharpe, Stacy T. Sims, Chad J. Macias, Geoff G. Futch, Michael D. Roberts, Jeffrey R. Stout, Michael J. Ormsbee, Alan A. Aragon, Bill I. Campbell, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak, Dominic P. D’Agostino, Michelle T. Barrack, Chad M. Kerksick, Richard B. Kreider, Douglas S. Kalman, Jose Antonio

Faculty Publications

Position statement: The International Society of Sports Nutrition (ISSN) provides an objective and critical review of the use of a ketogenic diet in healthy exercising adults, with a focus on exercise performance and body composition. However, this review does not address the use of exogenous ketone supplements. The following points summarize the position of the ISSN: 1. A ketogenic diet induces a state of nutritional ketosis, which is generally defined as serum ketone levels above 0.5 mM. While many factors can impact what amount of daily carbohydrate intake will result in these levels, a broad guideline is a daily dietary …