Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

Electronic Theses and Dissertations

Discipline
Institution
Keyword
Publication Year

Articles 631 - 660 of 3160

Full-Text Articles in Life Sciences

Manufacture Of Ingredients For Use In Clean Label Process Cheese Products, Ahmed Hammam Jan 2022

Manufacture Of Ingredients For Use In Clean Label Process Cheese Products, Ahmed Hammam

Electronic Theses and Dissertations

Micellar casein concentrate (MCC) is a high protein ingredient that can be used in several applications, such as manufacture of acid curd and process cheese products (PCP). Acid curd is one of the casein (CN) products, which can be obtained by precipitating the CN at a pH of 4.6 (isoelectric point) using starter cultures or direct acids. Acid curd has low mineral and calcium content due to the solubility of colloidal calcium phosphate at the isoelectric point in the whey. Acid curd and MCC can be utilized in manufacture of clean label PCP formulations. PCP is a dairy food prepared …


Defining Amino Acid Requirements Of Pregnant Sows: Challenges And Opportunities, Christian D. Ramirez-Camba Jan 2022

Defining Amino Acid Requirements Of Pregnant Sows: Challenges And Opportunities, Christian D. Ramirez-Camba

Electronic Theses and Dissertations

The efficient use of protein in animal production is dependent on the protein supply and its constituent amino acids (AA) in relation to the animal's needs. Excess AA are deaminated, and the resulting nitrogen (N) is excreted, whereas suboptimal AA intake reduces animal performance. Both increased nutrient excretion and decreased animal performance reduce the overall efficiency of the production unit. In sows, AA requirements should be adequate for optimizing reproductive performance, as measured, for example, by the number of pigs produced per sow per year while limiting N excretion. There is a desire to feed pregnant sows AA levels that …


Above-Ground Biomass Estimation In Oat Breeding Nurseries Using Uav-Based Multispectral Data, Rakshya Dhakal Jan 2022

Above-Ground Biomass Estimation In Oat Breeding Nurseries Using Uav-Based Multispectral Data, Rakshya Dhakal

Electronic Theses and Dissertations

Conventional methods of phenotyping for above-ground biomass under field conditions are time consuming, costly, and labor intensive. Unmanned Aerial Vehicles (UAV) offer a rapid and non-destructive approach for collecting high temporal and spatial resolution imagery of multiple field plots. Vegetation indices (VIs) can be derived from those imagery and have been used broadly for high-throughput biomass estimation, however, they suffer from spectrum saturation issues at high canopy density. In addition, VIs only reflect the canopy spectral information and fail to incorporate the 3-dimensional (3D) canopy structure and architecture information. It also ignores the spatial relationship of pixels to each other …


Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst Jan 2022

Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst

Electronic Theses and Dissertations

Because return to angler is an important outcome of put-and-take fisheries, understanding mortality of stocked Rainbow Trout is fundamental to managing these fisheries. Harvest rates of stocked Rainbow Trout Oncorhynchus mykiss in the Black Hills of South Dakota are believed to be below management objectives. Rainbow Trout not harvested by anglers are assumed to be lost to various sources of mortality, raising concerns about the cost of the Rainbow Trout stocking program relative to the benefit provided to anglers. (Simpson 2008). This study evaluated the factors influencing mortality of Rainbow Trout stocked into Black Hills reservoirs. We assessed the effects …


Optimization Of Electrically Driven Fluid Flow Through Tissue Hydrogels, Joseph C. Walker Jan 2022

Optimization Of Electrically Driven Fluid Flow Through Tissue Hydrogels, Joseph C. Walker

Electronic Theses and Dissertations

No abstract provided.


Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron Jan 2022

Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron

Electronic Theses and Dissertations

Ruminants and the microorganisms within their rumen have an intricate symbiotic relationship. Ruminants are able to convert plant materials that are indigestible to humans into meat, milk, and fiber for human consumption and use. Supplementing lipids in diets fed to beef cattle could be beneficial for the host animal as lipids are a higher density energy source. However, they can be detrimental to rumen microorganisms. While much research has been involved in elucidating the composition of the ruminal microbiome, knowledge of the effects of lipids on the ruminal microbiome has been quite limited. In this context, we took advantage of …


Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura Jan 2022

Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura

Electronic Theses and Dissertations

The tallgrass prairie of North America has changed drastically since colonial settlement, with up to 99% of this region converted for agriculture and other land-uses. Concurrent with grassland conversion, grassland birds have experienced the most extreme, consistent, and widespread population declines of any avian guild. Agricultural lands in the U.S. Midwest were able to provide adequate habitat for several bird species until the 1950’s; however, altered and intensified management practices have degraded much of remaining suitable habitat and undermined ecosystem functions. Consequently, many grassland birds have been identified as Species of Greatest Conservation Need (SGCN) in many State Wildlife Action …


Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr Jan 2022

Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr

Electronic Theses and Dissertations

Bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries) are members of the same genus and thus share multiple pathogens that can be spread between them. One specific respiratory pathogen of concern is Mycoplasma ovipneumoniae (M. ovipneumoniae), which has been linked to pneumonia epizootics in bighorn sheep that are often characterized by all age die offs upon initial exposure followed by years of low lamb recruitment. Domestic sheep have been identified as one carrier of M. ovipneumoniae with transmission between sheep species occurring when there is close contact on the landscape. To prevent this cross-species transmission, importance has been placed …


Preparation And Characterization Of Cellulose-Based Biodegradable Films From Switchgrass And Spent Coffee Grounds, Sajal Bhattarai Jan 2022

Preparation And Characterization Of Cellulose-Based Biodegradable Films From Switchgrass And Spent Coffee Grounds, Sajal Bhattarai

Electronic Theses and Dissertations

Plastic packaging materials, which take more than 700 years to degrade, have polluted land, water, and the atmosphere. The consequent environmental and life hazards require attention to alleviate plastic concerns. Developing plastic-replacing materials could be a viable option. To this end, lignocellulosic fraction from inexpensive and widespread agricultural biomass switchgrass (SG) and biowaste spent coffee grounds (SCG) offer a possible solution, mainly to prepare biodegradable packaging films. Herein, lignocellulosic fraction from SG and SCG has been extracted, solubilized in ZnCl2 solution, and crosslinked through Ca2+ ions to prepare gels and cast biodegradable films. The SG was subjected to three extraction …


Impact Of Storage And Different Variety On Proximate Composition And Functionality Of Lentils, Hussain Al Naser Jan 2022

Impact Of Storage And Different Variety On Proximate Composition And Functionality Of Lentils, Hussain Al Naser

Electronic Theses and Dissertations

Lentil (Lens culinaris) is an edible pulse and is a nutritious legume. The nutrient value and the application of lentils in other food resources have been well documented. However, there exists a lack of studies that focus on the storage impact of pulses on lentil composition and functionality. The focus of most studies has been on the composition of lentils while the composition relative to and functionality of stored lentils has not been sufficiently evaluated. Therefore, the objective of this research was to determine the effect of storage conditions on the nutrient composition and the functional properties of lentils. Four …


To Study The Factors Influencing The Sampling Variability On Microbiological Analysis Of Milk And Whey, Ratul Kalita Jan 2022

To Study The Factors Influencing The Sampling Variability On Microbiological Analysis Of Milk And Whey, Ratul Kalita

Electronic Theses and Dissertations

Sampling and analysis are integral parts of dairy processing in quality control, process monitoring and control, and meeting regulatory standards. Accurate sampling is vital to avoid erroneous analysis results and interpretation. The sampling process depends on the appropriateness of sample collection, transportation, storage, and analysis conditions. The bacterial heterogeneity and cluster formation, structural heterogeneity of food, sample unit and size, frequency, and sampling methods, are also crucial in sampling. We aimed to evaluate the influence of sample volume, sampling frequency, and sampling methods in experimental processing lines of whole milk, skim milk, and whey. To standardize the experimental protocols, a …


A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem Jan 2022

A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem

Electronic Theses and Dissertations

Lactose is the primary carbohydrate in most mammals' milk, commonly known as milk sugar. Milk permeate is a by-product of whey protein manufacturing through membrane technologies. It is cost-effective, available, and an excellent source of lactose. Sugar phosphorylation is a technique used to alter sugar's characteristics. It has numerous applications for developing dairy and food products. Lactose-6-phosphate (LP) is an organic compound that switches the hydrogen on lactose by monophosphate that has the potential to function as emulsifying salts (ES). ES, such as disodium phosphate (DSP) and trisodium citrate, have a critical effect on the emulsification characteristics of casein by …


Beta Glucan Enriched Oat-Wheat Naan - Nutritional, Sensory, And Rheological Evaluation Of Par-Baked Leavened Flatbreads, Anjana Adhikari Jan 2022

Beta Glucan Enriched Oat-Wheat Naan - Nutritional, Sensory, And Rheological Evaluation Of Par-Baked Leavened Flatbreads, Anjana Adhikari

Electronic Theses and Dissertations

No abstract provided.


Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben Jan 2022

Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben

Electronic Theses and Dissertations

Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …


Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das Jan 2022

Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das

Electronic Theses and Dissertations

Soil microbial communities are affected by many factors, such as soil nitrogen (N) and the quantity of grass-root exudates, changing seasons, fertilization method, plant diversity, and their origin. This holds for both natural and fertilized soils. This research was intended to expand the current understanding of soil microbial interactions associated with ecological functions. The objectives of the research were (I) to characterize prokaryotic soil communities, estimate functional potential, and quantify nitrogen cycle-associated prokaryotic gene expressions across three phases of the growing season, (II) to explore the diazotrophic community composition in a natural system using nifH sequencing as well as 16S …


Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst Jan 2022

Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst

Electronic Theses and Dissertations

Moisture extremes (excess and deficit) impact crop loss and water quality. Due to excessively wet springs and dry summers, crop damage can occur within the same county or field within the same year. To determine the magnitude of this problem in eastern South Dakota, indemnified crop insurance data for drought and excessive moisture claims were assessed for the years 1991-2020 for the occurrence of both excess moisture and drought in four counties in eastern South Dakota. Results show that there is greater than a 70% chance of the moisture extremes happening in the same year, making subsurface drainage, controlled drainage, …


Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi Jan 2022

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi

Electronic Theses and Dissertations

Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.


Economic Analysis Of Sunflower (Helianthus Annuus) Spacing In East Texas, Carly Wright Jan 2022

Economic Analysis Of Sunflower (Helianthus Annuus) Spacing In East Texas, Carly Wright

Electronic Theses and Dissertations

Two experiments were conducted on spacing of Helianthus annuus in East Texas to determine the spacing treatment that produces the greatest revenue per m2. The experiments had four spacing treatments of 8 × 15 cm, 15 × 15 cm, 23 × 23 cm, and 30 × 30 cm. Three different cultivars were used in each experiment to determine if there was an interaction between spacing and cultivars. In both experiments, the stem height, stem diameter, flower diameter, and disk diameter increased as spacing increased. Also, as spacing increased, the days-to-harvest decreased. The 8 × 15 cm spacing produced …


Influence Of Beef Carcass Weight On Carcass Chilling, Steak Case Life And Quality Traits, Trevor C. Dehaan Jan 2022

Influence Of Beef Carcass Weight On Carcass Chilling, Steak Case Life And Quality Traits, Trevor C. Dehaan

Electronic Theses and Dissertations

The objective of this thesis was to determine the influence of beef carcass weight on chilling and pH decline of beef carcasses, as well as color, case life and tenderness of steaks from the round, loin, rib and chuck. Twelve carcasses were allotted by hot carcass weight (HCW) into Heavyweight (HW) and Lightweight (LW) groups. Temperature decline, pH decline and thermal image temperature were measured in the round, loin, rib, and chuck for approximately 48 hours. Carcass data including 12th rib fat thickness, ribeye area, and marbling score were collected. The Semitendinosus (ST), Longissimus lumborum (LL), Longissimus thoracis (LT) and …


Phosphorus Use And Management Based On Fertilizer Placement, Rate Of Application, And Soil Biota In No-Till Situations, Brennan Alexander Bingham Lewis Jan 2022

Phosphorus Use And Management Based On Fertilizer Placement, Rate Of Application, And Soil Biota In No-Till Situations, Brennan Alexander Bingham Lewis

Electronic Theses and Dissertations

Phosphorus (P) pollution has become a concern among multiple scientific organizations as it leads to eutrophication, an algal bloom that depletes lacustrine and marine ecosystems of native species. Multiple strategies can be implemented to reduce phosphorus loss from agriculture fields, which is often implicated as a cause of eutrophication. Soil phosphorus chemistry results in phosphate fertilizers absorbing to clay minerals over time. Soil phosphorus is lost from agricultural fields primarily through wind and water erosion. No-till practices prevent soil erosion, which reduces the phosphorus from loading into waterways. Fertilizer placement affects phosphorus loss. Surface application of phosphorus fertilizers increases the …


Targeted Browsing With Goats For Eastern Redcedar (Juniperus Virginiana L.) Control, Alanna M. Hartsfield Jan 2022

Targeted Browsing With Goats For Eastern Redcedar (Juniperus Virginiana L.) Control, Alanna M. Hartsfield

Electronic Theses and Dissertations

As eastern redcedar (ERC) (Juniperus virginiana L.) grassland encroachment progresses, all potential control methods should be explored in the interest of Great Plains grassland health and longevity. Targeted browsing with goats has been proven as an effective control method on some juniper species; however, little is known about its ability to control ERC. These studies intend to mend knowledge gaps of how targeted browsing with goats control ERC by causing tree death without chemicals or machinery. The first study is two 3x3 Latin squares comparing protein-supplemented diets. The second study is a randomized complete block design of five 0.224 ha …


Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal Jan 2022

Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal

Electronic Theses and Dissertations

Phomopsis stem canker (PSC), caused by species of Diaporthe, is a major disease of sunflower (Helianthus annuus L.) in the U.S., which can cause yield loss over 40%. The disease prevalence increased since 2010 in the major sunflower producing U.S. states of Nebraska, Minnesota, North Dakota, and South Dakota. Previous studies on the use of foliar fungicides in managing Phomopsis stem canker indicated mixed results on their efficacy in disease reduction and yield gain. Additionally, we do not have sufficient information on asymptomatic colonization by endophytic Diaporthe on sunflower tissue and how foliar fungicide and weather parameters affect the prevalence …


Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire Jan 2022

Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire

Electronic Theses and Dissertations

Oat is an important cereal crop grown worldwide. Oats have the potential to contribute to human health due to their unique nutritional attributes. Developing oat cultivars with efficient root systems able to extract heterogeneously distributed soil resources can help maintain yield under drought conditions and in nutrient poor soil. Various root traits determine the soil volume that is explored by the root system for resource acquisition. Knowledge about the genetic control of oat root traits and response to biotic and abiotic environmental factors is lacking. Identifying quantitative trait loci associated with root traits and understanding the response of roots to …


Determining The Impact Of Corn Source On Nutrient Availability And Pig Performance, And Improving Boar Performance During Heat Stress, Jordan Jansen Jan 2022

Determining The Impact Of Corn Source On Nutrient Availability And Pig Performance, And Improving Boar Performance During Heat Stress, Jordan Jansen

Electronic Theses and Dissertations

The quality and effectiveness of the feedstuffs included in diets of production livestock directly impact yield efficiency and performance characteristics desired by producers. This thesis focuses on the performance and digestibility of corn from different countries in pig diets and how different feed additives can be supplemented to improve boar reproductive performance during heat stress. Corn is the largest energy source in common pig diets. The physical characteristics of corn such as broken kernels, foreign material, and excessive dust are said to negatively impact the quality of the grain for pig diets by being less digestible and a worsened nutrient …


Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini Jan 2022

Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini

Electronic Theses and Dissertations

Phomopsis stem canker is a major threat to sunflower (Helianthus annuus L.) production worldwide and its prevalence has increased from 1.5% in 2001 to 61.0% in 2015 in the sunflower producing U.S. states of Colorado, Kansas, Minnesota, Nebraska, North Dakota, South Dakota, and Texas. The disease causes early senescence and lodging of sunflower plants resulting in yield losses up to 40% and oil content up to 25%. Several species of Diaporthe have been reported to cause Phomopsis stem canker. In the United States, Diaporthe helianthi Munyt.-Cvetk. et al. and D. gulyae Shivas et al., have been reported as predominant causal …


Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri Jan 2022

Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri

Electronic Theses and Dissertations

Long-term tillage and crop rotation systems are important agricultural management practices as these can have direct impact on the soil’s key properties. The objectives of this study were to (ⅰ) quantify the soil pore characteristics under long-term tillage and crop rotation using X-ray computed tomography (XCT) and to assess the relationships between XCT-measured pore parameters and soil hydro-physical properties; and (ⅱ) evaluate the impacts of long-term tillage and crop rotation on select soil health indicators. The objective (ⅰ) was carried out at Haskell Agricultural Laboratory (HAL), Concord, NE; and objective (ⅰi) was carried out at South Central Agricultural Laboratory (SCAL), …


Impact Of Storage Conditions And Varieties On The Composition, Physical Properties, And Functionality Of Dry Peas, Sushmita Karki Jan 2022

Impact Of Storage Conditions And Varieties On The Composition, Physical Properties, And Functionality Of Dry Peas, Sushmita Karki

Electronic Theses and Dissertations

Dry peas are sustainable, healthy, and nutritious pulse crop and are excellent source of protein and complex carbohydrates as well as micronutrients. The effects of storage conditions on composition of beans and cereals have been widely reported. The information gained contributed significantly to better storage practices for these commodities. However, literature addressing the chemical composition, functionality, and physical characteristics of dry pea seeds upon storage is scarce. This study was designed to address the impact of storage conditions and varieties on the nutrient profile, functionality, and physical properties of dry pea seeds. Six different varieties of dry peas were stored …


Production Of Calcium Reduced Micellar Casein Concentrate Powders And Their Use In Process Cheese Products, Anil Kommineni Jan 2022

Production Of Calcium Reduced Micellar Casein Concentrate Powders And Their Use In Process Cheese Products, Anil Kommineni

Electronic Theses and Dissertations

Protein is an essential dietary component, and sufficient intake is vital in a healthy and balanced diet. Consumers are becoming increasingly aware of and knowledgeable about the role of protein in the diet. Two of the next-generation dairy protein ingredients isolated from milk are micellar casein (MCC) and milk-derived whey protein, isolated from skim milk using microfiltration (MF). Membrane filtration has been used extensively by the dairy industry to produce a variety of dairy ingredients from milk. MCC manufactured from freshly pasteurized milk can be directly consumed or as a supplement to fortify and enhance nutritional qualities in processed food …


It's Not Just No-Till: Crop Rotations Are Key To Improving Soil Quality And Grain Yields At Dakota Lakes Research Farm, Natalie Sturm Jan 2022

It's Not Just No-Till: Crop Rotations Are Key To Improving Soil Quality And Grain Yields At Dakota Lakes Research Farm, Natalie Sturm

Electronic Theses and Dissertations

No-till is increasingly promoted as a vital agricultural practice to address the challenge of improving soil quality while maintaining the ability to produce food, feed, fuel, and fiber. However, questions remain regarding 1) how different crop rotations impact soil quality under no-till management specifically and 2) whether significant improvements in soil quality can be made without negatively impacted crop yields. In this study, we demonstrate that even under the same long-term no-till management, differences in grain yields and soil biological, chemical, and physical properties exist between crop rotations at Dakota Lakes Research Farm in central South Dakota. We propose that …


Crispr/Cas9 Whole-Genome Screens Identify Genes Critical For Neutral Lipid Metabolism In Macrophages, Rifat Sultana Jan 2022

Crispr/Cas9 Whole-Genome Screens Identify Genes Critical For Neutral Lipid Metabolism In Macrophages, Rifat Sultana

Electronic Theses and Dissertations

Lipid-laden macrophages contribute to atherosclerotic plaque formation in atherosclerosisrelated heart diseases. Thus, understanding formation and degradation of lipid would help identify therapeutic targets for the treatment of atherosclerosis. In the first chapter of this dissertation, I have explained the current understanding of cholesterol metabolism in macrophages and how mTOR could play a potential role in this process. Additionally, I have described how CRISPR/Cas9 whole-genome screens present an effective tool to identify genes regulating cholesterol metabolism including positive and negative regulators of lipid droplet formation and lipid droplet degradation. In Chapter 2, I have described the results of the CRISPR/Cas9 whole-genome …