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Articles 301 - 330 of 3160
Full-Text Articles in Life Sciences
Genetic And Ecologic Effects Of Seed Dormancy Genes On Seed Aging, Soil Seedbank Longevity, And Microbiome In An Isogenic Background Of Rice ( Oryza Sativa L.), Bhupinder Singh Batth
Genetic And Ecologic Effects Of Seed Dormancy Genes On Seed Aging, Soil Seedbank Longevity, And Microbiome In An Isogenic Background Of Rice ( Oryza Sativa L.), Bhupinder Singh Batth
Electronic Theses and Dissertations
No abstract provided.
Whole Genome Crispr-Cas9 Screens Identify Genes Contributing To Viral Entry And Phagocytosis, Md Saddam Hossain
Whole Genome Crispr-Cas9 Screens Identify Genes Contributing To Viral Entry And Phagocytosis, Md Saddam Hossain
Electronic Theses and Dissertations
CRISPR/Cas9 is a revolutionary technique for genome editing where Cas9 nucleases can be targeted to specific genomic sites by single-guide RNAs (sgRNAs) containing roughly 20-nucleotide sequences that are complementary to the target region. Double strand breaks created by Cas9 are repaired through non-homologous end-joining (NHEJ) in most cells, resulting in insertions and deletions leading to frame shift mutations in coding regions. Thus, the CRISPR-Cas9 system can be exploited for systematic evaluation of gene function relationships by whole genome screening. We also developed a CRISPR whole‐genome screen and counter‐screen strategy based on a pseudoviral platform that allowed identification of genes specific …
Impacts Of Starter Fertilizer On Corn Development, Nutrient Uptake And Grain Yield In Eastern South Dakota, Larousse Dorissant
Impacts Of Starter Fertilizer On Corn Development, Nutrient Uptake And Grain Yield In Eastern South Dakota, Larousse Dorissant
Electronic Theses and Dissertations
Cold and wet spring conditions during planting in the Northern Corn Belt can limit nutrient mobility, especially phosphorus (P) due to reduced mineralization. This often triggers the use of starter fertilizers to enhance nutrient availability within the root zone, and as a result improve nutrient uptake and the overall plant growth. Albeit research has shown that starter fertilizer applications can significantly boost corn grain yield potential, the most substantial yield responses from starter fertilization are typically observed in fields where soil test P levels fall below the critical soil test P threshold. This dissertation explores i) the effects of starter …
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Electronic Theses and Dissertations
Various technologies and alternative ingredients are being developed to increase the nutrient value of fibrous feedstuffs, improve animal performance, and promote sustainable swine production. Fiber-degrading enzymes have been extensively studied in growing pigs, with few studies focused on gestating sows. When considering gestating sows have greater energy digestibility of fibrous feedstuffs than grower pigs, the efficacy of fiber-degrading enzymes needs to be determined for implementation in commercial sow diets. As fiber inclusion in gestation diets benefits sow performance, corn dried distiller grains with solubles (DDGS) are a candidate fiber source for gestation alternative energy ingredient. Functional fibers, such as yeast …
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Electronic Theses and Dissertations
Chickpea (cicer arietinum) samples from five distinct varieties (crown, royal, orion, sierra, and frontier) were subjected to varying storage conditions to investigate the impact of temperature and relative humidity (rh) on their chemical, functional, physical properties, and vitamin b profile. over 360 days, samples were stored at two temperatures (21°c and 40°c) and three rh levels (40%, 55%, 65%), with collections made every 90 days. initial observations focused on key chemical properties including moisture, protein, fat, and total starch content. subsequently, protein and starch fractions were isolated for further analysis, employing techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) …
Artificial Intelligence And Image-Based Phenotyping For Monitoring Morpho-Physiological Dynamics In Grapevines, Prakriti Sharma
Artificial Intelligence And Image-Based Phenotyping For Monitoring Morpho-Physiological Dynamics In Grapevines, Prakriti Sharma
Electronic Theses and Dissertations
Plant phenotypic features are essential for determining the structure, composition, and function of plants. These traits are controlled by both environmental variables and genetic makeup. Conventional manual methods of phenotyping, although time-consuming and not very accurate, have laid the foundation for more modern approaches such as high throughput phenotyping (HTP). HTP utilizes imaging, sensor networks, robotics, and data analytics to automate the process of collecting and analyzing data. This study utilizes hyperspectral and digital imaging techniques to quantify physiological, biochemical, and morphological characteristics in grapevines. Hyperspectral imaging was leveraged to collect spectral data for evaluating physiological factors, whereas digital imaging …
Effects Of Isoacids Supplementation In Lactating Cows’ Diet Varying In Forage Fiber Level On Performances, Feed Efficiency, And Milk Fatty Acids Profile, Sumon Ahmed
Electronic Theses and Dissertations
No abstract provided.
Evaluation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross
Electronic Theses and Dissertations
Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Electronic Theses and Dissertations
Objective: Quantify South Dakota (SD)’s capacity to procure locally-sourced fruit and vegetables (FVs) for K-12 school meals.
Methods: Secondary analysis of 2017 SD agricultural census and school meal data. Yields were converted to edible cups of FVs using school meal guidelines provided by the U.S. Department of Agriculture. Student enrollment and meal redemptions were used to estimate the number of FV cups needed.
Results: Estimated SD FV production met minimum total K-12 cup requirements, except for total fruit (66%) and dark green vegetables (80%). Legumes supplied 77% of the total cups of vegetables produced in 2017, whereas dark green vegetables …
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Electronic Theses and Dissertations
Managing weeds, improving soil health, and reducing the use of plastic mulch continue to be priorities for South Dakota vegetable farmers. Farmers have expressed an interest in integrating cover crops into their farm systems. Clover cover crops used as a living mulch within and along cash crop rows may aid in weed suppression, nitrogen fixation, and prevent soil erosion. However, prior research has shown challenges of incorporating living mulch due to yield decreases. Research conducted in 2022 and 2023 in eastern South Dakota investigated the effects of four clover and four in-row soil management treatments on broccoli and organic winter …
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Electronic Theses and Dissertations
Soil health is influenced by climate change, cultural practices, topography, crop rotations, cover crops, soil parent materials, and soil biota. Interactions between these factors can improve or reduce soil health. This thesis investigates two topics, using soil sensors to provide a rapid assessment of soil health, and the impact of cover crops on soil biological activity and greenhouse gas emissions. Chapter 1 explores the use of two types of sensors to measure apparent electrical conductivity (ECa) and provides examples on the use of these sensors. Even though both sensors measure ECa using different processes, they provide useful information about temporal …
Nitrogen Fertilizer Placement And Rate Impacts Sunflower Seed Yield, Oil And Protein Content, Rojina Kafle
Nitrogen Fertilizer Placement And Rate Impacts Sunflower Seed Yield, Oil And Protein Content, Rojina Kafle
Electronic Theses and Dissertations
South Dakota accounts for about 36% of the total US commercial sunflower production and nitrogen is a major input. Costs related to N fertilizer demand economically optimum N rates. This study investigated the impact of the N fertilizer placement method (broadcast vs band) and rate (0, 28, 56, 84, 112, 140 kg N ha-1) on leaf chlorophyll content, head diameter, seed yield, seed oil, and protein concentration of three sunflower hybrids in 2022 and 2023. The experiment was conducted at Brookings (eastern SD- conventional till, 66 cm rainfall) using an oil-type hybrid and at central SD sites (no-till, 57 cm …
Increasing Soy Products Inclusion In Swine Diets To Improve Growth Performance, Health, And Pork Quality, Ashir Foreman Atoo
Increasing Soy Products Inclusion In Swine Diets To Improve Growth Performance, Health, And Pork Quality, Ashir Foreman Atoo
Electronic Theses and Dissertations
Despite its excellent nutritional profile, soybean meal (SBM) is limited in nursery pig diets due to its anti-nutritional factors (ANFs), which young pigs tolerate poorly. In the first study, strategies to mitigate these effects included supplementation with enzymes, alone or with acidifiers. A total of 240 weaned pigs (5.9 ± 0.8 kg average body weight [BW]) were assigned in randomized complete block design to five dietary treatments: low, medium, or high SBM diets, high SBM with multienzyme, and high SBM with multienzyme plus acidifier. Diets were fed in three phases: phase 1 (0–1 weeks), phase 2 (1–3 weeks), and phase …
Translocations Of North American Bison Across Large Climatic Gradients: Impacts On Thermoregulation, Body Size, And Helmintic Infection, Hayden Wolfe
Electronic Theses and Dissertations
Every year, one-eighth of all North American bison (Bison bison) are translocated, often across large climatic gradients. Over the last 16 years bison have been translocated from Wind Cave National Park in South Dakota to establish six herds across the United States central grasslands in Iowa, Missouri, Illinois, Indiana, and Kansas. Influence of climatic gradients on herd-level thermoregulation, body size, and helmintic infection were evaluated. Overall, 1174 thermal images and 1050 fecal samples were collected between 2021 and 2023. Physiological heat flux was used to measure thermoregulation, asymptotic body mass (ABM) was used for body size, and helminth genera abundance …
Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson
Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson
Electronic Theses and Dissertations
The objective of this thesis was to determine the influence of beef hot carcass weight on proteolysis to better understand early postmortem proteolysis. Beef tenderness formation is influenced by various factors, such as temperature decline and proteolysis. Limited focus has been given to the effects of carcass weight on proteolysis. This study aimed to investigate the influence of increased beef hot carcass weight (HCW) on proteolysis of four muscles (serratus ventralis, longissimus thoracis, longissimus lumborum, and semitendinosus) during early postmortem aging times. Beef steers (n =12) were harvested and categorized into two weight groups: lightweight (LW; HCW = 349 ± …
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Electronic Theses and Dissertations
Stream connectivity is crucial for fish movement and genetic diversity in fragmented landscapes. Road-crossings often act as barriers, disrupting hydrology and isolating fish populations. The Southeast Aquatic Resources Partnership (SARP) has developed rapid road-crossing assessment protocols and tools to prioritize restoration projects. However, managers lack tools for planning, directing, and initiating roadcrossing assessments. Managers also require modeling approaches to effectively identify and monitor road-crossings across dynamic stream networks. Presented here is a case study offering a roadmap for effective implementation of collaborative road-crossing assessments and prioritization of remediation projects, alongside an exploration of predictive modeling approaches to identify problematic crossings …
Elucidating The Role Of Sialic Acid In Tumorigenic Pathways, Kakali Das
Elucidating The Role Of Sialic Acid In Tumorigenic Pathways, Kakali Das
Electronic Theses and Dissertations
Hypersialylation is a prognostic biomarker in cancer cells. The upregulated sialic acid expression on cancer cells facilitates tumorigenesis by playing a critical role in cancer cell proliferation and growth, immune evasion, cell signaling, and metastasis by interacting with various carbohydrate-binding molecules. Sialic acids on cancer cell surface undergo various modifications like O-acetylation at positions 4,7,9 de-acetylation and addition of glycolyl group at position 5. In our first chapter we analyzed the effect of de-acetylated sialic acid on migration via selectin binding in colon cancer cell line HCT116 and in lung cancer cell line A549. Selectins are calcium-dependent cell adhesion molecules …
Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga
Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga
Electronic Theses and Dissertations
Weaning is a necessary step in pig production. After weaning, pigs are subjected to various nutritional, psychological, and environmental stresses. The weaning period is an important period for the intestinal development of the pig. During this time, digestion, immunity, metabolism, and various other aspects of the pig change rapidly. Therefore, providing high-quality feed ingredients is crucial to encourage the newly weaned pig to start consuming solid feed. Two independent studies were conducted to determine if the inclusion of: 1) corn fermented protein (CFP) or 2) probiotics or postbiotics impact nursery pigs' growth performance and gut health. The first experiment utilized …
The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter
The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter
Electronic Theses and Dissertations
Phosphorus (P) management is an ongoing challenge in modern agriculture. Due to the importance of P in agriculture, P fertilizer additions are made throughout the U.S. to avoid nutrient-depleted soil. However, the application of P is problematic, as an excess of P in an agricultural system elevates the risk of environmental degradation, and current synthetic P sources are a fleeting resource. Additionally, a majority of P found in agricultural soils are insoluble, in the form of secondary and primary P minerals, making this large pool of P unavailable to plants. Fortunately, soil fungi organisms, such as arbuscular mycorrhizal fungi (AMF) …
Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema
Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema
Electronic Theses and Dissertations
Sheep production in the United States is a popular venture for both small farmers and large enterprise owners, as there is a market for end products such as wool and meat. One major factor of raising sheep is nutrition as an adequate diet will allow an animal to reach its full genetic potential for growth. With that in mind, the composition of the diet must be formulated to benefit the animal. Ruminants have the ability to use a variety of plants that are often inedible for humans to create products that humans can consume. These varying feedstuffs, whether they include …
Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu
Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu
Electronic Theses and Dissertations
With increasing demand for soybean, maximizing plant yield is important yiel is dependent on nitrogen availability, but nitrogen fertilizers can meet the plant's needs but have severe environmental impacts. An economically and environmentally sustainable approach is to enhance nitrogen availability through Biological Nitrogen Fixation by symbiotic rhizobia, Bradyrhizobium, an efficient nitrogen fixer for soybean. This symbiosis begins with attachment of rhizobia to legume roots. Root and rhizobia surface properties play a significant role in this attachment. Another major factor affecting root colonization is bacterial competition ability. There is a lack of knowledge on the role of bacterial surface properties, competition …
Development And Characterization Of Transparent Soil Using Biodegradable Polysaccharides, Said Badshah
Development And Characterization Of Transparent Soil Using Biodegradable Polysaccharides, Said Badshah
Electronic Theses and Dissertations
No abstract provided.
Investigating The Role And Mechanism Of Host Cell Death In Porcine Reproductive And Respiratory Virus Infection, Michelle Steyn
Investigating The Role And Mechanism Of Host Cell Death In Porcine Reproductive And Respiratory Virus Infection, Michelle Steyn
Electronic Theses and Dissertations
No abstract provided.
Portable X-Ray Fluorescence Spectrometry For Sensing Salinity And Sodicity In Glacial Northern Great Plains Soils With Machine Learning Models, Adam Devlin
Electronic Theses and Dissertations
Saline and sodic soils are an increasing concern across the Northern Great Plains (NGP) due to factors of climate change and land management that are drawing geologically derived salts to the land surface. Traditional laboratory assessments, such as electrical conductivity (EC) and sodium adsorption ratio (SAR), are time and resource consumptive. Portable X-ray fluorescence (PXRF) may be a viable proximal sensing alternative, as it is able to provide elemental data in minutes, in situ or ex situ, and can directly quantify salinity-associated elements like Ca, Mg, and S. PXRF paired with predictive models has proven useful for a range of …
The Spatial Ecology Of Plains Spotted Skunks In South Dakota: Insights From Species Distribution, Resource Selection, And Co-Occurrence Models, Kara M. White
Electronic Theses and Dissertations
The plains spotted skunk (Spilogale interrupta) is a data-deficient small carnivore native to the central plains of North America that has experienced significant population declines. My dissertation investigates the species’ distribution, habitat associations, and interspecific interactions in South Dakota, providing crucial information for conservation actions. In Chapter 1, I developed ensemble distribution models on a broad scale, incorporating species location data from 1985–2020, environmental factors, and six predictive modeling algorithms. I identified key predictors including mean temperature diurnal range and proportion of area as pasture, and estimated ~31,300 km2 of potential habitat, predominately in eastern South Dakota. In Chapter 2, …
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Electronic Theses and Dissertations
The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …
Role Of Interferon Induced Transmembrane Protein 3 (Ifitm3) And Neural Precursor Cell Expressed Developmentally Down Regulated Protein 4 (Nedd4) In Seneca Virus A (Sva) Replication, Shamiq Aftab
Electronic Theses and Dissertations
In this study, we studied the role of IFITM3 in SVA replication in vitro by silencing the endogenous IFITM3 in NCI-H1299 cells. An average of 1.94-fold increase in SVA VP2 expression and an average of 4.4-fold increase in supernatant virus titer was observed after 24h SVA isolate SD15-26 infection. We have indeed observed an increase in viral mRNA copies by an average of 5-fold in IFITM3 silenced NCI-H1299 cells at 8 hpi indicating that IFITM3 restricts SVA replication post virus attachment and entry. A positive correlation between interferon-alpha induced IFITM3 upregulation and reduced SVA replication was observed. To further confirm …
Soil Responses To Eastern Redcedar (Juniperus Virginiana L.) Afforestation And Prescribed Fire In South-Central South Dakota, Robert Schaefer
Soil Responses To Eastern Redcedar (Juniperus Virginiana L.) Afforestation And Prescribed Fire In South-Central South Dakota, Robert Schaefer
Electronic Theses and Dissertations
Research was conducted in 2019 and 2020 on rangelands located in south-central South Dakota assessing a number of physical, chemical, and biological properties of mineral ustepts and ustents soils associated with eastern redcedar (ERC) (Juniperus virginiana) afforestation and prescribed fire. ERC afforestation appeared to be associated with significantly higher field-condition soil aggregate stability, cation exchange capacity (CEC), soil pH, and fungi:bacteria (F:B) as well as significantly lower soil moisture, nitrate-N, phosphorus, bacteria biomass, and Gram-positive:Gram-negative (GP:GN) bacteria compared to native grassland conditions, while other mineral soil properties appeared relatively unaffected. Compared to soil conditions under dense, living ERC canopies, prescribed …
Regulatory Landscapes: Transcriptome And Microrna Regulated Senescence And Dormancy In Grapevines And Gene Regulatory Networks In Nitrogen-Fixing Soybean Nodules, Ramandeep Kaur
Electronic Theses and Dissertations
Grapevine and soybean are two economically important crops, each with unique yet equally crucial physiological processes that profoundly influence their growth and productivity. Grapevine (Vitis sp.), a temperate fruit crop, employs dormancy to withstand winter challenges and can grow in various regions worldwide. Understanding the molecular mechanisms involved in dormancy in Vitis species, is crucial for improving grapevine selection for resilience in northern temperate regions. Dormancy induction is modulated by decreasing photoperiods in some Vitis species, enhancing their ability to prepare for winter stress conditions. Understanding the molecular underpinnings of response to photoperiod will provide potential markers for selecting genotypes …
Impacts Of Cover Crops And Diversified Crop Rotations On Soil Biochemical Properties, Hydrophysical Characteristics, And Water Dynamics In Long-Term No-Till Cropping Systems, Sainfort Vital
Electronic Theses and Dissertations
This research assessed the impacts of crop rotations and cover crops on the soil's biochemical and hydrophysical properties within long-term no-till systems. The research was conducted at the Southeast Research Farm, near Beresford, South Dakota. The crop rotations contain two levels: 4-year corn (Zea mays L.)-soybean (Glycine max L.)-oat (Avena sativa L.)-rye (Secale cereale L.) (CSOR) and 2-year corn-soybean (CS) rotations. The cover crop factors also had two levels within each rotation treatment: cover crops (CC) and no cover crops (NCC). All plots were managed under long-term no-till (NT), with soils mapped as Egan silty clay loam (fine-silty, mixed, superactive, …