Life Sciences Commons^™

The Effects Of Gibberellic Acid, Smoke Water, And Cold Stratification On The Germination Of Asclepias And Liatris Seeds, Jemima Essien

Electronic Theses and Dissertations

Seed dormancy presents a significant challenge in the propagation of many native perennial species, including members of the Asclepias and Liatris genera. This study evaluated the effects of cold stratification and chemical treatments on the germination performance of six Liatris species and five Asclepias species using water (control), gibberellic acid (GA), smoke water (SW), or a combination of both (GA+SW) as chemical treatments. The primary objective was to determine whether smoke water could significantly enhance germination and serve as a viable pre-treatment method for horticultural use. Results indicated that stratification significantly enhanced germination, with stratified seeds achieving an average germination …

Intraspecific Genetic Variation In Green Salamanders (Aneides Aeneus) At Bays Mountain Park, Brianna Drake

Electronic Theses and Dissertations

Accurate taxonomy is crucial for conserving vulnerable cryptic species like the green salamander (Aneides aeneus) in the Southern Appalachian Mountains. Prior studies identified three distinct lineages within the A. aeneus complex: Northern Appalachians, Southern Appalachians, and Blue-Ridge Escarpment. One previously collected sample indicated green salamanders in northeast Tennessee fit within the Northern lineage. The present study uses molecular data of A. aeneus at Bays Mountain Park (BMP) in Kingsport, Tennessee, to determine where the BMP population fits within these lineages. It was hypothesized that individuals from BMP belong in the northern lineage. Tail tips were collected from thirty-four …

Investigating The Effect Of Fasting On Senescent Cells In Nothobranchius Furzeri Fish, Israel A. Akinola

Electronic Theses and Dissertations

Senescent cells (SCs) have been documented to accumulate with age in the organism. Recent studies have shown that reducing the number of SCs in an organism result in tissue rejuvenation and extension of life expectancy. Intermittent fasting is reported to have multiple health benefits and is speculated to reduce the burden of SCs. Nothobranchius furzeri are a new model organism for studying aging and for which there are short- and long-lived strains available. We hypothesize that long- and short-lived strains of N. furzeri will accumulate SCs at different rates; and that fasting will reduce the burden of SCs.

The results …

Development, Optimization, And Validation Of A High-Sensitivity Capillary Zone Electrophoresis System For Bioanalytical Applications, Aaron I. Mena

Electronic Theses and Dissertations

Capillary zone electrophoresis (CZE) is a powerful analytical technique widely used for biomolecular separation due to its high resolution, efficiency, and sensitivity. This report presents the development and validation of a novel CZE system designed to improve modularity, reproducibility, and operational efficiency. The system integrates a laser-induced fluorescence (LIF) detection method with a 488 nm laser, enhanced fluid control through a negative pressure system, and a user-friendly GUI for automated operation and data acquisition. Performance verification included pressure stability tests, fluorescence accuracy assessments, and electrophoretic reproducibility studies. Comparative analysis with a previous CZE iteration confirmed improved baseline stability, peak resolution, …

Synthetic Protein Mimetic Based Therapies For Neurodegeneration, Nicholas H. Stillman

Electronic Theses and Dissertations

Protein-protein interactions (PPIs) are crucial for the regulation of a majority of, if not every fundamental cellular process. Despite their role in regular biological processes, dysregulated and/or aberrant protein-protein interactions (aPPIs) are often related to the onset of disease including cancer, viral infection, and amyloid diseases. aPPIs have historically been deemed ‘undruggable’ due to their large surface area and lack of a binding cavity; however, today, more than 40 disease-related aPPIs have been targeted with small molecules and several of those have reached clinical trials.

A class of synthetic protein mimetics called oligopyridylamides (OPs) has been shown to inhibit disease-related …

Advancing Tka Biomechanics: From Joint Simulator To Boundary Condition Development, Yashar Ali Behnam

Electronic Theses and Dissertations

This dissertation addresses four specific aims that collectively attempted to advance the experimental and computational analysis of total knee arthroplasty (TKA) components. The first study focused on the development of a novel whole knee joint simulator capable of simultaneous tibiofemoral and patellofemoral knee loading. This simulator employs custom fixturing to facilitate dynamic, unconstrained, muscle-driven PF articulation alongside controlled TF contact mechanics. Validation against experimental measurements showed strong agreement, demonstrating the simulator's potential as a valuable tool for future TKA design and surgical technique investigations.

The second study verified implant-specific physiological boundary conditions which accurately simulate activities of daily living using …

Effects Of Essential Oils Alone Or In Combination With Monensin In An In Vitro Model Of Ruminal Acidosis, Jorge L. Bonilla Urbina

Electronic Theses and Dissertations

The objective of this study was to evaluate the effect of essential oils and/or monensin on the concentration of volatile fatty acids (VFA), ammonia, and pH in a ruminal acidosis in vitro model. Red Angus steers (n = 4; BW = 435 ± 9 kg) with ruminal and duodenal cannulas were allocated in a 4 × 4 Latin square design. Treatments were 1) Fed no essential oils (RB) or monensin sodium (MON:CON); 2) Fed RB at a rate of 14 g daily with no Mon (RB+); 3) Fed no RB and fed Mon at 400 mg daily (MON+); 4) Fed …

Integrating Invasion Risk And Habitat Suitability To Guide Invasive Species Management In Wetlands, Lakes, And Rivers, Brenden M. Elwer

Electronic Theses and Dissertations

Invasive species disrupt ecosystems by altering habitats, competing with native species, and disrupting food webs. The northern Great Plains is an ecologically important region with many wetlands, lakes, and rivers that are under threat from two invasive species, Bighead (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix; bigheaded carp). These large planktivores change the abundance and composition of phytoplankton and zooplankton communities which alters prey for native mussels, fishes, and, potentially, waterfowl. Bigheaded carps have invaded a portion of the Northern Great Plains, and preventing further spread is a primary management goal. My work assists these efforts by providing tools to …

Effects Of Storage Conditions On The Composition, Physical Properties, And Functionalities Of Peas And Lentils, Fatimah Alsaeed

Electronic Theses and Dissertations

Peas (Pisum sativum L.) and lentils (Lens culinaris) are nutritionally rich pulses valued for their high protein, fiber, and starch content. Although their composition and health advantages are widely known, little is known about the effects of long-term storage on their functional qualities and nutritional value. The purpose of this study was to investigate the impact of storage conditions and duration on the proximate composition, physical attributes, and techno-functional traits of lentils and peas. Storage conditions included bin (BIN; -30 to 41°C, 20–98% RH), non-environmentally controlled warehouse (NECW; -23 to 32°C, 25–84% RH), and environmentally controlled warehouse (ECW; 19–21°C, 50–53% …

Assessment And Management Of Dectes Stem Borer, Dectes Texanus Leconte, In South Dakota Soybeans, Zachary Loomis

Electronic Theses and Dissertations

The Dectes stem borer (Dectes texanus LeConte, Coleoptera: Cerambycidae) has emerged as an increasingly significant pest of soybean (Glycine max) in South Dakota. Although historically considered a minor concern, rising populations of this insect have led to significant preharvest lodging and subsequent yield loss in affected fields. The Dectes stem borer's larval feeding within the soybean stem interferes with nutrient flow, and as the season progresses, this internal feeding weakens stem integrity, amplifying lodging risks and causing considerable challenges for farmers and researchers. Since 2017, both the number and severity of Dectes stem borer infestations have been on the rise …

Innovative Approaches To Enhancing The Functional Properties Of Dairy Products: Evaluating Water Hydration Effects On Fat-Free Mozzarella Baking Performance And Impact Of Hyaluronic Acid On The Functional Properties Of Milk, Suresh Ganpatbhai Sutariya

Electronic Theses and Dissertations

In recent years, the dairy sector has experienced a significant rise in the healthy eating trend, with consumers increasingly seeking products that are fat-free, free from added sugars, and high in protein. Additionally, specialized health dairy items enriched with functional ingredients such as probiotics, prebiotics, and bioactive peptides are gaining traction for their added health benefits. This dissertation covers three studies in two domains: the first on water hydration's impact on fat-free mozzarella baking, and the second on the influence of hyaluronic acid, and its combination with kappa-carrageenan, on the physical properties of milk. One such product, Fat-free mozzarella is …

Evaluating The Success Of The Rev It Up! Curriculum Training On The Self-Efficacy Of Sdsu Extension Expanded Food And Nutrition Education Program (Efnep) & Supplemental Nutrition Assistance Program - Education (Snap-Ed) Paraprofessional Educators In Teaching Adolescents, Oluwatemilorun Akinsanya

Electronic Theses and Dissertations

Objective: Evaluate self-efficacy changes among Extension paraprofessional nutrition educators after receiving an adolescent curriculum (Rev It Up!) training. Methods: Paired t-tests on three adapted scales measured changes at pre- (T1), post- (T2), and 1 yr post-intervention (T3): the Teacher Interpersonal Self-Efficacy Scale (TISS), Nutrition Teaching Self-Efficacy Scale (NTSES), and Self-Efficacy and Nutrition Teaching Methods scale (SNTMS). Results: Six educators completed the TISS and NTSES; 5 completed the SNTMS. The TISS showed significant improvement from T1 to T2 only (p= 0.03); the NTSES showed improvement between T1 and T2 (p= 0.01) and T1 and T3 (0.01); and there were no significant …

Evaluation Of Established Perennial Clover Living Mulch And Soil Management Effects On Weed Suppression, Soil Health, And Broccolini Production For The Northern Great Plains, Connor Ruen

Electronic Theses and Dissertations

Vegetable cropping systems have long been reliant on the use of tillage to prepare favorable seedbeds and manage weed pressure. Tillage degrades soil structure, decreases water infiltration and leads to compaction. Due to recent droughts across the central United Stated, it is important for water conservation to improve infiltration and avoid run off and erosion. Changes in weather patterns not only influence drought conditions but bring heat waves that have made early and late planted cool season crops in the brassica family prone bolting, leading to unmarketable produce and economic losses for farmers. This 2023 and 2024 study was designed …

Ovarian Response, Endocrine Environment, And Pregnancy-Associated Glycoproteins In Beef Cows Using The 7 & 7 Synchronization Protocol, Hunter Haberman

Electronic Theses and Dissertations

This Review aims to provide an overview of the bovine estrous cycle and pregnancy establishment, beginning with follicular development, luteolysis, maternal recognition of pregnancy and early embryonic development. Manipulating the estrus cycle with synchronization protocols is a critical aspect of the beef industry and has greatly improved the efficiency of artificial insemination. This review will evaluate various strategies to synchronize estrus from the very basic to more advanced synchronization protocols. There are many factors that affect the success of the protocol, and many studies have been done to evaluate the estrus cycle to reach the highest level of success from …

Quantification Of Auxin-Cytokinin Response During Primary Root And Lateral Root Development In Common Bean, Gurdeep Singh

Electronic Theses and Dissertations

Roots support water and nutrient uptake, anchorage, and soil interactions through continuous growth at the primary root tip (PRT). In common bean (Phaseolus vulgaris), new cells are produced in an open root apical meristem and subsequently elongate in the elongation zone, and together these processes drive root tip progression through the soil. Lateral roots (LRs) arise in the differentiation zone, where pairs of pericycle founder cells dedifferentiate, divide asymmetrically, and form lateral root primordia (LRP) that pass through defined developmental stages before emergence. Auxin and cytokinin (CK) are central regulators of both primary root growth and LR development, yet their …

Programming Carcass Composition Through Gestational Nutrition, Sydni Elise Borders

Electronic Theses and Dissertations

The objective of this study was to evaluate the influence of maternal nutrient restriction during mid-gestation on the performance of the dam, feeding performance, carcass composition, and genetic expression in the offspring. Cows (n = 46) were randomly assigned to one of two treatments based on global nutrient intake: 1) fed to achieve and/or maintain a BCS of 5 or 6 (Positive Energy Status [PES]; n = 24) or 2) fed to lose one BCS during 99 days of mid-gestation (Negative Energy Status [NES]; n = 22). Cows in NES had greater decreases (P < 0.05) in body fat stores and muscle mass, elevated NEFA concentrations, and displayed decreased (P < 0.05) DMI, HP, and GHG emissions. There were no differences in milk components or volume produced between treatments (P > 0.05). There were no differences in …

Impact Of Minimally Processed Red Meat On Biomarkers Of Cognitive Decline, Saba Vaezi

Electronic Theses and Dissertations

No abstract provided.

Evaluation Of High-Moisture Ear Corn As A Roughage Source In Finishing Diets Fed To Beef Steers, Riley J. Leeson

Electronic Theses and Dissertations

The objective of this study was to evaluate the feedlot performance, rumination activity, and carcass characteristics of high moisture ear corn (HMEC) as a roughage source in finishing diets fed to beef steers. Backgrounded steers (n = 191; initial shrunk weight [4% BW] = 449 kg  23.9 kg) were assigned randomly to 1 of 24 pens (8 steers/pen) with pens randomly assigned to one of four dietary treatments (6 pens/treatment): control diet with hay fed at 10% roughage equivalency (RE; HAY10), or HMEC at either 14% (EAR14), 10% (EAR10), or 6% (EAR6) RE. High-moisture ear corn was assumed to …

Finishing Location And Forage Diversity Influences Carcass Characteristics, Meat Quality Attributes, Terroir, And Color Stability Of Grass-Finished Bison Bulls, Garrett Tyler Weldy

Electronic Theses and Dissertations

The overall objective of this thesis is to determine the influence of finishing location and forage diversity on carcass characteristics, meat quality, terroir, and color stability of grass-finished bison bulls. The specific objectives were 1) Determine the effect of finishing location on carcass characteristics, meat quality attributes, and terroir of grass-finished bison bulls, 2) Investigate the effect of forage diversity on the carcass characteristics, meat quality attributes, and terroir of grass-finished bison bulls, and 3) Determine the effect of finishing location on the meat color stability of grass-finished bison bulls. Overall, results indicate that finishing location influenced carcass characteristics, objective …

The Influence Of Bottom-Up And Top-Down Effects On The Density And Distribution Of Common Leopards In The Eastern Himalayas, Karma Choki

Electronic Theses and Dissertations

Leopards (Panthera pardus fusca) are widely distributed across Asia, yet their conservation is overshadowed by the conservation status of other flagship predators like tigers (Panthera tigris), in the Eastern Himalayas. Despite their ability to coexist with other large carnivores, leopard populations are shaped by intricate ecological interactions and increasing human pressures. This thesis investigates the abundance and density, habitat use, and drivers of persistence of leopards across Bhutan to inform multi-species carnivore conservation planning. My first chapter provides an overview of the ecological roles of large carnivores and how they persist on the landscape. I review the mechanisms that facilitate …

Comparative Evaluation Of Fungal Strains For The Bioprocessing Of Soybean Hulls Using Cheese Whey As Fermentation Medium, Ruchita Bhattarai

Electronic Theses and Dissertations

Globally, soybean is the most widely cultivated oilseed crops, and serve as the primary source of cooking oil, contributing to around 50% of global oilseed production. During the industrial processing of soybean oil, a significant amount of soybean hull (SH) is produced, which makes up about 8% of the grain yield, resulting in approximately 18–20 million tons of hulls, one of the largest by-products in the soybean industry. The hulls have limited commercial value and are primarily used as fiber additives in dairy cattle feed, but a large portion is still discarded as agricultural waste. Similarly, cheese whey (CW), a …

Quantifying Landscape Variability Of Dynamic Soil Properties In The James River Valley, Eastern South Dakota, Sage Reuter-Schmitt

Electronic Theses and Dissertations

Dynamic soil properties (DSP) are a great tool for monitoring land-use change, and they are frequently used as soil health metrics. The NRCS and other soil researchers often use repeating, single-point or nested-point DSP studies to monitor and better understand the effects of land management decisions. While these studies capture the temporal variability of DSPs, they overlook the field-scale spatial variability of DSPs. Soil organic carbon (SOC) provides critical services to the ecosystem and other environments. These traits, as well as its susceptibility to management decisions, make SOC an important soil health indicator and DSP. Measuring SOC is often time …

The Spatial And Temporal Dimensions Of Plant–Soil Feedback In Native Perennial Monocultures, Bret Lang

Electronic Theses and Dissertations

Plant-soil feedbacks (PSFs) describe how plants change their soil environment in ways that can either negatively or positively affect subsequent plant growth. In doing so, they offer an opportunity to connect ecological processes with agricultural practice. My dissertation examines PSFs created by native perennial monocultures, commonly used for native seed production, across time and space, and through soil legacies. Specifically, I focus on how plant species identity shapes both soil nutrient and soil microbial community responses. To address these questions, I conducted field experiments with monocultures of five forb species native to South Dakota and established plots with these species …

Impacts Of Early/Mid-Gestation Shearing On Umbilical Blood Flow And Lamb Birth Weight, Mara Johnson

Electronic Theses and Dissertations

Studies outside the USA have reported that shearing pregnant ewes during mid to late gestation increases lamb birth weight. Our hypothesis was that shearing pregnant ewes on day 50 of gestation would increase umbilical blood flow (UBF), fetal size, lambing and placental weights. Twenty-eight Polypay ewes were randomly assigned to two treatments: shorn (S) and sham-shorn (Control, CON) and divided in singleton (S: n=4; CON: n=4), twins (S: n=5; CON: n=5) and triplets (S: n=5; CON: n=5). Umbilical BF and fetal and placental measurements were obtained via ultrasonography every 10 days from day 50 to 110 of gestation. Lamb birth …

Isolation And Characterization Of Rhizobia Bacteria From Midwest Soils, Jr Loya

Electronic Theses and Dissertations

Symbiotic bacteria, known as rhizobia, can colonize the roots of legume plants and then convert atmospheric nitrogen into a usable form by the host through the process called biological nitrogen fixation (BNF). Many of these bacteria produce other plant-growth-promoting benefits, such as nutrient acquisition, phytohormone production, and the modification of plant stress responses. Agricultural inoculants made from these bacteria have helped reduce the need for man-made nitrogenous fertilizers, known causes of pollution and eutrophication. There are additional benefits in developing bacteria inoculants local to regional soils. The ability to survive and compete in Midwest soil and climates present an opportunity …

Physiological And Genetic Studies Of Hydrotropism In The Maize Primary Root, Muyu Gu

Electronic Theses and Dissertations

No abstract provided.

Evaluating Dietary Consequences On Body Mass And Thermal Heat Flux Of North American Bison (Bison Bison) Yearling Heifers, Kendall Marie Wald

Electronic Theses and Dissertations

No abstract provided.

Evaluating Effects Of Copper Sources In Nursery Pig Diets And Phytase Inclusion And Diet Interactions On Water Usage In Growing And Finishing Pigs, Daniel Rosales

Electronic Theses and Dissertations

In modern swine production, nutritional strategies that optimize growth performance, resource utilization, and carcass quality are essential. Three independent trials were conducted to evaluate how dietary modifications involving copper (Cu) sources, phytase inclusion, and amino acid reformulation affect nursery and grow-finish pigs. The first trial assessed the effects of three different Cu sources on nursery pig growth performance. A total of 1,145 pigs (5.9 ± 1.3 kg BW) were assigned to one of four dietary treatments: control, tri-basic Cu chloride source #1 (TBCC1), Cu chelate (cCu), or tri-basic Cu chloride source #2 (TBCC2), each providing 150 ppm of Cu. Over …

Deciphering Genetic Contributions To Antibiotic Persistence: Insights From Multi-Gene Knockout Studies, Elise K. Schweer

Electronic Theses and Dissertations

Antibiotic resistance is a critical global health challenge, complicating efforts to treat bacterial infections effectively. Antibiotic persistence plays a significant role in driving resistance. Persisters are a subpopulation of non-dividing bacterial cells that often evade lethal antibiotic treatment. Once antibiotics are removed, these cells "resurrect," resuming growth and may lead to relapsing infections. Notably, the progeny of persister cells exhibit a heightened tendency to acquire mutations or incorporate antibiotic resistance genes, further compounding the challenge of antibiotic resistance. In our recent study, we knocked out seven hypothetical genes and observed a significant reduction in persister levels. Compared to the wild …

Optimizing Heifer Development On Dormant Rangelands By Integrating Precision Livestock Technologies With The Range Supplementation Model And The Evaluation Of Short-Term Rotations Of Precision Technology On Plant Communities, Hadley Ann Dotts

Electronic Theses and Dissertations

Heifer development on dormant rangelands offers a cost-effective alternative to feedlot or drylot systems. However, traditional bunk supplementation methods often result in uneven intake, inefficient feed use, and ecological degradation around static feeding sites. This thesis evaluated the integration of precision livestock technologies and a dynamic Range Supplementation Model to improve individual animal performance, weight uniformity, and rangeland sustainability in extensive heifer development systems in the Northern Great Plains. The first study involved a seven-month trial (November 2023–May 2024) with 50 yearling Angus heifers grazing dormant native rangelands at the SDSU Cottonwood Field Station. Heifers were stratified into two treatments: …