Life Sciences Commons^™

Assessing Listeria Risk During Different Stages Of Ice Cream Manufacturing And Storage, Neha Singh

Electronic Theses and Dissertations

Listeriosis is a life-threatening infection caused by foods contaminated with Listeria monocytogenes. Some of the major ice cream recalls in recent years reaffirm the ability of this food-borne pathogen to survive in diverse dairy processing environments and cause cross contamination. Inspection reports revealed certain lapses in implementing adequate hygienic practices for Listeria persistence in the processing environment, leading to cross contamination of ice cream. The higher levels of cross contamination of raw ice cream mix might result in random heat-injured cells when exposed to minimum heat treatment (69°C for 30 min). Evidence about the presence of injured cells in ice …

The Efficacy Of Protein Supplementation On Attenuating Muscle Atrophy Following Disuse In The Collegiate Population, Leah Kroeger

Electronic Theses and Dissertations

The purpose of this study is to determine the effects of a protein supplementation protocol through a period of muscular disuse in maintaining muscle mass, strength, jump force production, and cross-sectional area in the collegiate population. Two groups of healthy collegiate participants underwent two weeks of unilateral lower limb suspension (ULLS), during which the control group consumed a normal diet and the treatment group received an additional 75 g of protein supplementation. Lean mass, strength, and force production were measured between dietary intakes at baseline and following immobilization. Muscle area was lost between both groups following ULLS (treatment, - 282.8 …

Identification And Characterization Of Stress Responsive Genes In Soybean And Sunflower, Surendra Neupane

Electronic Theses and Dissertations

Stress responsive genes encode proteins involved in plants’ response to abiotic and biotic stresses. Among such stress responsive proteins, proteins encoded by resistance genes (R genes) or nucleotide binding site-leucine-rich repeats (NBS-LRRs) and mitogen-activated protein kinases (MAPKs) are the major groups of proteins regulating biotic and abiotic stresses, respectively. Previous studies in Nepal’s lab at SDSU identified and characterized coiled coil (CC)-NBS-LRRs (CNLs), resistance to powdery mildew8 (RPW8)-NBS-LRRs (RNLs), NBS-LRR (NLs), and MAPK proteins in soybean. This study focuses on R and MAPK genes in the recently sequenced genome of sunflower as well as the toll-interleukin-1 receptor-like nucleotide-binding site leucine-rich …

Effects Of Oats And Distiller’S Dried Grains Fibers On Fortification Of Asian Noodles – Evaluation Of Glycemic Response, Noodle Quality And Nutritional Composition, Kara Konst

Electronic Theses and Dissertations

The objective of this study was to develop Asian wheat noodle formulations enriched with oat flour and corn DDG at 10, 20 and 30 % wheat flour replacement levels and to evaluate the effects of this fiber and protein enrichment on the glycemic response of human subjects. We hypothesized that the addition of high-fiber oats and high-fiber, high-protein DDG will have synergistic effects in lowering the glycemic response in human subjects. In this study, steamed instant Asian noodles were prepared with DDG(D) and oats(OF) in combination with all-purpose wheat flour(W) in the following flour blends: (W:D) 90:10, (W:OF) 90:10, (W:D) …

Effect Of Water Velocity And Temperature On Energy Use, Behavior, And Mortality Of Pallid Sturgeon Scaphirhynchus Albus Larvae, Joseph Thomas Mrnak

Electronic Theses and Dissertations

Pallid Sturgeon Scaphirhynchus albus are a federally endangered species endemic to the Missouri River basin. Natural reproduction has been limited for decades and a recruitment bottleneck is hypothesized to occur during the larval stage of development. Understanding factors that affect survival of Pallid Sturgeon larvae is key given their critical status and ongoing recovery efforts. In this study, I evaluated the effects of water velocity and temperature on energy use, behavior, and mortality of endogenously feeding Pallid Sturgeon larvae (< 25 mm TL). In the first experiment, I examined the effect of water velocity (0 – 8.3 cm s⁻¹) on larval growth, energy density, swimming activity, and mortality. Larval growth rate at 16.3 °C was similar across …

Effect Of Mineral Ions On The Functional Properties Of Starch Films, Aliza Sigdel

Electronic Theses and Dissertations

Essential minerals are indispensable inorganic micronutrients that modulate vital physiological functions at the molecular level. Their deficiency, though required in small amounts, impairs health significantly. Their supplementation through diet is prudent and carbohydrates standout as favorable choice. Carbohydrates, starches and polysaccharides, exhibit unique chemical structures and functionalities, and interact with mineral ions in several ways. The aim of this research is to investigate the effect of mineral ions on the physicochemical properties, tensile strength and in vitro starch digestion of starch-films. Corn starch and potato starch along with Fe2+, Mn2+, Cu2+ and Zn2+ ions have been chosen as model starch …

Menstrual Cycle And The Vascular Properties Of Remote Preconditioning, Shelby Rachel

Electronic Theses and Dissertations

PURPOSE: A promising therapeutic intervention to counteract endothelial ischemia/reperfusion (I/R) injury is ischemic preconditioning (IPC). Limited data from ovariectomized animal models shows that estrogen deficiency abolishes the cardioprotective effects of IPC, whereas estrogen replacement restores protection. This suggests that alterations in female sex hormones, particularly estrogens, that occur naturally during the menstrual cycle is likely to influence the vascular protective properties of IPC. The aim of the present study was to identify how changes in female sex hormones that occur naturally during the menstrual cycle interfere with IPC to provide vascular endothelial protection in women. METHODS: Nine healthy premenopausal eumenorrheic …

Level Of Satisfaction Among Food Pantry Clients, Staff/Volunteers, And Directors And Its Association With Client Choice In Food Pantry Layouts, Myranda Dunmire

Electronic Theses and Dissertations

Objectives: Food insecurity is an issue in rural communities, particularly among food pantry clients. Food pantries may not provide clients with the opportunity to choose their own foods (client choice), and instead may just give a set amount and type of foods. Client choice is important in regards to client health status as well as perceptions of autonomy and quality of life. Although client choice can be promoted in food pantries, it may be difficult to grasp the process of food pantry transitioning and how well changes such as these may be perceived by clients or food pantry personnel. We …

A Sustainable Future In The Implementation Of Clinical Pharmacogenomics, Nicholas Keeling

Electronic Theses and Dissertations

Purpose: The sustainability of clinical pharmacogenomics requires further study of clinical education on the topic, its effects on clinical workflow, and the responsibilities of different providers for its delivery. Tools from the discipline of implementation science were utilized herein to help achieve the purposes of the three studies. The broad purpose of this dissertation is to advance the work of clinical pharmacogenomic implementation through a more rigorous convergence with implementation science.

Methods: Three studies constitute the whole of this dissertation. The first is a scoping review that provides a broad characterization of the methods utilized in available peer-revieliterature focusing on …

Continuous Production Of Raloxifene Hydrochloride Loaded Nanostructured Lipid Carriers Using Hot-Melt Extrusion Technology, Derick Muhindo

Electronic Theses and Dissertations

The aim of this study was to utilize a continuous process for the production of Raloxifene Hydrochloride (RX-HCl) loaded NLC formulations for extended drug release using hot-melt extrusion technology coupled with probe sonication, and also to evaluate the in vitro characteristics of the prepared NLCs by particle size, PDI, zeta potential, entrapment efficiency, drug loading, and in vitro drug release profile. Preparation of the NLCs using HME technology involved two main steps, first formation of a pre-emulsion after extrusion and then size reduction of the pre-emulsion using probe sonication to obtain the NLCs. Process parameters for the extrusion process like …

The Role Of Dunc13 In Ethanol Tolerance, Yusuke Wachi

Electronic Theses and Dissertations

Although the effects of ethanol on presynaptic activity have been studied, the molecular mechanisms and the changes in gene expression which are responsible for inducing ethanol tolerance are unclear. Munc13-1 is an active-zone protein that is essential for presynaptic vesicle fusion. This protein interacts with vesicle fusion machinery at presynaptic active zones in the mammalian brain. The C1 domain of Munc13-1 binds diacylglycerol (DAG), which helps membrane localization of this protein and promotes vesicle fusion, facilitating synaptic vesicle release. Previously, it was shown that ethanol binds to the C1 domain of Munc13-1 in vitro at concentrations below 100 mM (Das …

Acute Exercise And Creativity: Embodied Cognition Approaches, Emily M. Frith

Electronic Theses and Dissertations

This dissertation manuscript is the culmination of three years of research examining several unique, exercise-induced mechanisms underlying creativity. This collection of work addresses historical and current empirical concepts of creativity in a narrative review, providing recommendations for future research. Several reviews follow this introduction, highlighting the proposed effects of exercise on creativity, putative mechanisms for creativity, and the effects of exercise and embodied manipulations on creative behavior. Multiple experiments utilizing moderate-intensity exercise as a theoretical stimulus for higher-order cognitions were conducted to investigate associations between exercise and creativity, which lead to the final dissertation experiment. The dissertation experiment was the …

Developing Zebrafish As An In Vivo Model To Screen Compounds For Anti-Cancer Activity In Human Breast Cancer, Trisha Dhawan

Electronic Theses and Dissertations

Breast Cancer (BC) is the most frequently diagnosed cancer; 1:8 women are at risk of developing BC in her lifetime. Cancer metastasis causes the majority of deaths in BC patients. Moreover, side effects of traditional chemotherapeutic drugs (TCD) impair the quality of life of these patients. Discovery and development of safe and effective new therapies is imperative for the treatment of BC and targeting metastasis. The goal herein is to further expand the applicability of in vivo xenotransplantation of human BC cells in transgenic zebrafish to screen potential chemotherapeutics for toxicity and efficacy. For xenotransplantation, MCF-7, BT-474, and MDA-MB-231 BC …

Effects Of Impoundments On The Community Assemblage And Gene Flow Of Stream Crayfishes, Zanethia Choice Barnett

Electronic Theses and Dissertations

Dams and their impoundments block movement of stream organism and change stream physiochemical properties, which subsequently changes biological assemblages and creates barriers to gene flow. While changes in species assemblages and gene flow have been assessed for numerous impoundments and stream organisms, no study has assessed the effects of large impoundments on crayfish assemblages and population genetic diversity and connectivity. I examined the physiochemical, biological, and genetic effects of impoundments on crayfishes. Between May 2015 and August 2017, I sampled multiple sites up- and downstream of three impounded streams, and along the lengths of two unimpounded streams, in the Bear …

Agronomic And Quality Response Of Hard Red Spring Wheat Genotypes To Management Systems In South Dakota, Jonathan Kleinjan

Electronic Theses and Dissertations

Intensive cereals management techniques such as multiple fungicide applications and in-season split N applications have been used to successfully improve wheat yields in Europe and in some winter wheat production areas of the USA. However, research on the effects of these management practices and their interaction with genotypes is limited in the hard red spring wheat (HRSW) production areas of the USA. The objectives of this study were to: (i) compare management treatments and (ii) quantify any interaction effects between management and genotype on the agronomic characteristics and relevant flour and dough properties of locally-adapted HRSW genotypes. A randomized complete …

Impact Of Concussion And Orthopedic Injuries On Physical Activity And Quality Of Life After Sport Retirement, Makenna Hancock

Electronic Theses and Dissertations

CONTEXT: Concussions and orthopedic injuries represent a significant risk related to participation in collegiate athletics. These injuries can create significant long-term impairments and functional limitations which may also decrease former athletes’ quality of life (QoL) and inhibit their ability to engage in physical activity. OBJECTIVE: To investigate QoL and physical activity levels of former collegiate athletes with a history of concussion and orthopedic injury, orthopedic injury only, and healthy controls. DESIGN: Descriptive, experimental study.
SETTING: Daily life as former National Collegiate Athletic Association Division I collegiate athlete.
PARTICIPANTS: Former Division I collegiate athletes competing in football, women’s soccer, baseball, softball, …

Production And Storage Stability Of High Concentrated Micellar Casein And Its Effect On The Functional Properties Of Process Cheese Products, Ahmed Hammam

Electronic Theses and Dissertations

Micellar casein is a high-protein ingredient that can be used in process cheese products (PCP) formulations. PCP is a dairy food prepared by blending dairy ingredients (such as natural cheese, protein concentrates, butter, non-fat dry milk NFDM, whey powder, and permeate) with nondairy ingredients (such as sodium chloride, water, emulsifying salts, color, and flavors) and then heating the mixture with a continuous agitation to produce a homogeneous product with an extended shelf-life. The first objective of this study was to produce a highly concentrated micellar casein (HC-MC) and evaluate its storage stability. Skim milk was pasteurized at 76°C for 16 …

A Bioinfomatic Analysis Of Luminal And Mucosal Ileal Bacteria Colonizing Nursery Pigs Fed Different Protein Sources, Jamie L. Ortman

Electronic Theses and Dissertations

Weaning is a critical time in a young pig’s life that will greatly impact its adult growth and development. The objective of this research was to evaluate the effects of various protein sources on the bacterial composition within luminal and mucosal populations in simple and complex diets to gain a better understanding of factors influencing gut health. Weaned pigs were fed 1 of 3 simple experimental diets: 1) basic diet containing corn and soybean meal (Negative Control - NEG), 2) basic diet + fishmeal (FM; Positive Control - POS), and 3) basic diet + microbially-enhanced soybean meal (MSBM). Phase I …

Developing Food Quality Standards For Distiller’S Dried Grains – Evaluating Composition Quality And Safety, Tanvee Deshpande

Electronic Theses and Dissertations

Introduction: Distillers Grains represent a major co-product of corn ethanol production. At the production rate of 44 million tons/year and a price of $95/ton, this co-product has potential as a food ingredient (solvent-treated dried products) owing to its high protein content (38%) and high fiber content (40%TDF). The aim of the research was to determine the composition, quality and safety of several initial moisture types of distiller’s grains – distiller’s grains without solubles (DG) and distiller’s grains with solubles (DGS) in wet and freeze-dried form (FrD) from 2017 and 2018.
Method: Processing methods were optimized for raw DG & DGS …

Undoing Gender: An Analysis Of How Women Communicate Within The Agricultural Industry, Shala R. Larson

Electronic Theses and Dissertations

In recent history, women have entered more business-like positions in the agricultural industry. This thesis seeks to understand how women in agriculture describe their workplace communication and whether they feel they are part of a muted group. Twelve women were interviewed regarding their experiences as women in agriculture and were asked whether they feel valued in their positions and how they communicate with their male co-workers. Specifically, interviewees were asked if they ever felt the need to alter their communication to complement, emulate, or otherwise adjust to masculine communication styles. The majority of women interviewed reported having to strategically choose …

Diversifying Cornfields By Interseeding Cover Crops: Practical Implications And The Response Of Invertebrate Communities, Michael Bredeson

Electronic Theses and Dissertations

The lack of suitable habitat conditions for beneficial organisms in simplified agroecosystems leads to unstable invertebrate communities and overreliance on chemical control of herbivores. It is possible to manage pest populations without agrichemicals by manipulating farmland so that herbivores are impaired by plant-driven bottom-up and enemy-driven top-down antagonisms. Interseeding cover crops between established crop rows is a method used by farmers to improve habitat suitability for natural enemies and hinder host-finding, feeding and movement by herbivores. Here I address three important research gaps related to interseeding cover crops. A calcium carbonate seed coating, used to improve seed-broadcasting efficiency, was tested …

Effects Of Energy Development On Movements, Home Ranges, And Resource Selection Of White-Tailed Deer In The Western Dakotas, Bailey S. Gullikson

Electronic Theses and Dissertations

Oil and natural gas development has increased in recent years and research is needed to assess potential impacts on white-tailed deer (Odocoileus virginianus) populations. Our objectives were to document movements, home ranges, and resource selection of female white-tailed deer in response to energy development in the western Dakotas. Our study areas included Dunn County, North Dakota, an area with current oil and gas development, and Grant County, North Dakota, and Perkins County, South Dakota, areas without current oil and gas development. We captured and fitted 150 female deer with Very High Frequency (VHF) collars across study sites, and …

Estimating Soil Organic Carbon In Cultivated Soils Using Soil Test Data, Remote Sensing Imagery From Satellites (Landsat 8 And Plantscope), And Web Soil Survey Data, Muhammed Halil Koparan

Electronic Theses and Dissertations

Soil organic carbon (SOC) is an important soil parameter of cultivated soils that needs to be monitored and mapped regularly to enhance soil health and productivity. SOC levels in cultivated areas is difficult to monitor for farmers and is costly to analyze using traditional methods. The objective of this study was to estimate surface SOC distribution in selected soils of Major Land Resource Areas (MLRA) 102A (Rolling Till Plain, Brookings County, SD) and 103 (Central Iowa and Minnesota Till Prairies, Lac qui Parle County, MN) using soil sample data, Web Soil Survey (WSS) data, and satellite imagery (Landsat 8 and …

Stoichiometric Homeostasis In Two Native And Two Invasive South Dakotan Grasses, Joshua Thonas Harvey

Electronic Theses and Dissertations

Increased nutrient availability has been widely linked to the success of invasive plants, however a general mechanism explaining these observations is lacking. Stoichiometric homeostasis (H), which is the regulation of internal nutrient concentrations, has been used to explain changes in plant community diversity under alterations in nutrient availability. One hypothesis holds that plants with high regulation (larger H) decrease in abundance in nutrient enriched conditions but are stable in nutrient deficient and drought conditions, likely due to extensive root systems. Additionally, plants with low regulation (lower H) increase in abundance under nutrient enriched conditions but are sensitive to drought …

Species Of Fusarium Causing Root Rot Of Soybean In South Dakota: Characterization, Pathogenicity, And Interaction With Heterodera Glycines, Paul Nyawanda Okello

Electronic Theses and Dissertations

In South Dakota, Fusarium-associated diseases (e.g. seedling diseases, root rot and sudden death syndrome) are emerging threats to soybean production. Several species of Fusarium have been reported to cause these diseases in the soybean producing regions of the United States and in the world. However, little information is available on the species of Fusarium causing soybean root rot in South Dakota and their pathogenicity. Therefore, the objectives of this study were to (1) characterize the species of Fusarium causing soybean root rot in South Dakota; (2) evaluate the cross-pathogenicity of species of Fusarium causing root rot of soybean and corn; …

Impacts Of Small Intestinal Enzyme Activity And Varying Corn Grain Processing Types On Feeding Behavior, And Growth Performance Of Finishing Beef Steers, Wyatt Smith

Electronic Theses and Dissertations

The objective of this study was to quantify the differences in activity of jejunal maltase and isomaltase between 2 groups of steers with average dry matter intake (DMI) and differing average daily gain (ADG). Dry matter intake and ADG were measured in crossbred steers (n = 69). Jejunal mucosal samples were collected from 8 steers with the greatest (high) or least (low) ADG and average DMI (± 0.55 standard deviation). Homogenates of jejunal mucosa were incubated with increasing amounts of maltose and isomaltose to determine the disaccharidase kinetics. Neither the Km of isomaltase (P = 0.15) or maltase (P = …

The Economic And Social Values Associated With Small South Dakota Lakes, Aaron Patrick Sundmark

Electronic Theses and Dissertations

The valuation of small fishing lakes is a vital component in understanding the importance of fishing and of recreational resources, in general. Knowing the values associated with such lakes is essential when prioritizing management activities. The overall value of a lake as a resource for human benefit is estimated as the summation of both instrumental and non-instrumental values. Instrumental values consist of economic and utilitarian values, as well as the values that a lake provides from ecosystem services. Non-instrumental values consider what the lake is worth as a good of its own, such as aesthetic, moral, and spiritual values gained …

Physical Activity Environments In Rural Communities: Exploring The Relationship Between Community Perceptions And The Environment, Elizabeth M. Bye

Electronic Theses and Dissertations

Purpose: To assess the physical activity environment, community perceptions of the physical activity environment, and the relationship between these variables in rural and/or underserved communities with high obesity prevalence.
Methods: The Rural Active Living Assessment (RALA) was used to assess the programs and policies (PPA), town-wide amenities (TWA), and street segments (SSA) of the physical activity environment and the Rural Active Living Perceived Environmental Support Scale (RALPESS) was used to assess community perception of the physical activity environment within eleven rural and/or underserved communities. Each section of the RALA and RALPESS are further broken down into additional subsections in order …

Effects Of Weight Loss On Gut Microbiota, Samitinjaya Dhakal

Electronic Theses and Dissertations

Incidence of obesity continues to rise for several decades now. Despite the availability of the multiple weight loss programs, losing or maintaining weight remains a universal challenge. A comprehensive lifestyle modification is usually the cornerstone for achieving weight loss. A wealth of new information on gut microbial responses to diet to impact human health has introduced a new frontier in nutrition science that may help better understand inter-individual response variations to weight-loss interventions. This observational study examined the microbiota changes after weight-loss in relation to age, sex, and body-composition features in adults. A 10% reduction in body weight resulted in …

Assessing Freshwater Mussel (Bivalvia: Unionidae) Assemblages And Effects Of Eutrophication On Pyganodon Grandis In Lakes Of Eastern South Dakota, Katherine M. Wollman

Electronic Theses and Dissertations

Globally, the family Unionidae is the most threatened group of freshwater fauna. South Dakota is well known for its agricultural production, leading to cultural eutrophication from enhanced fertilizers and sediments. This impact can alter species presence, age, growth and mortality. Objectives of this study were to 1) implement the first comprehensive lake survey of freshwater mussels in eastern South Dakota lakes and document distribution, occurrence and relative abundance, 2) evaluate relationships between age, growth and mortality of Pyganodon grandis and lake trophic state. Freshwater mussels were sampled in 2017 from 116 proportionally and randomly selected natural lakes and reservoir basins …