Life Sciences Commons^™

A Tmeff2-Regulated Cell Cycle Derived Gene Signature Is Prognostic Of Recurrence Risk In Prostate Cancer, Constantin Georgescu, Joshua M. Corbin, Sandra Thibivilliers, Zachary D. Webb, Yan D. Zhao, Jan Koster, Kar-Ming Fung, Adam S. Asch, Jonathan D. Wren, Maria J. Ruiz-Echevarria

Department of Agronomy and Horticulture: Faculty Publications

Background: The clinical behavior of prostate cancer (PCa) is variable, and while the majority of cases remain indolent, 10% of patients progress to deadly forms of the disease. Current clinical predictors used at the time of diagnosis have limitations to accurately establish progression risk. Here we describe the development of a tumor suppressor regulated, cell-cycle gene expression based prognostic signature for PCa, and validate its independent contribution to risk stratification in several radical prostatectomy (RP) patient cohorts.

Methods: We used RNA interference experiments in PCa cell lines to identify a gene expression based gene signature associated with Tmeff2, an …

Impact Of Hurricane Katrina On The Coastal Systems Of Southern Louisiana, Wen-Ching Chuang, Tarsha Eason, Ahjond Garmestani, Caleb Roberts

Department of Agronomy and Horticulture: Faculty Publications

Natural disasters, such as hurricanes and forest fires, could trigger collapse and reorganization of social-ecological systems. In the face of external perturbations, a resilient system would have capacity to absorb impacts, adapt to change, learn, and if needed, reorganize within the same regime. Within this context, we asked how human and natural systems in Louisiana responded to Hurricane Katrina, and how the natural disaster altered the status of these systems. This paper discusses community resilience to natural hazards and addresses the limitations for assessing disaster resilience. Furthermore, we assessed social and environmental change in New Orleans and southern Louisiana through …

Genome-Wide Association Study For Multiple Biotic Stress Resistance In Synthetic Hexaploid Wheat, Madhav Bhatta, Alexey Morgounov, Vikas Belamkar, Stephen N. Wegulo, Abdelfattah A. Dababat, Gul Erginbas-Orakci, Mustapha El Bouhssini, Pravin Gautam, Jesse Poland, Nilufer Akci, Lutfu Demir, Ruth Wanyera, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genetic resistance against biotic stress is a major goal in many wheat breeding programs. However, modern wheat cultivars have a limited genetic variation for disease and pest resistance and there is always a possibility of the evolution of new diseases and pests to overcome previously identified resistance genes. A total of 125 synthetic hexaploid wheats (SHWs; 2n = 6x = 42, AABBDD, Triticum aestivum L.) were characterized for resistance to fungal pathogens that cause wheat rusts (leaf; Puccinia triticina, stem; P. graminis f.sp. tritici, and stripe; P. striiformis f.sp. tritici) and crown rot (Fusarium spp.); …

Expression Of The Arabidopsis Wrinkled 1 Transcription Factor Leads To Higher Accumulation Of Palmitate In Soybean Seed, Pamela A. Vogel, Shen Bayon De Noyer, Hyunwoo Park, Hanh Nguyen, Lili Hou, Taity Changa, Hoang Le Khang, Ozan N. Ciftci, Tong Wang, Edgar B. Cahoon, Thomas Elmo Clemente

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed-specific expression cassette carrying the Arabidopsis transcription factor WRINKLED1 (AtWRI1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the AtWRI1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl-ACP thioesterase (GmFatA) resulted …

Statistical And Machine Learning Methods Evaluated For Incorporating Soil And Weather Into Corn Nitrogen Recommendations, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, D. Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) fertilizer recommendation tools could be improved for estimating corn (Zea mays L.) N needs by incorporating site-specific soil and weather information. However, an evaluation of analytical methods is needed to determine the success of incorporating this information. The objectives of this research were to evaluate statistical and machine learning (ML) algorithms for utilizing soil and weather information for improving corn N recommendation tools. Eight algorithms [stepwise, ridge regression, least absolute shrinkage and selection operator (Lasso), elastic net regression, principal component regression (PCR), partial least squares regression (PLSR), decision tree, and random forest] were evaluated using a dataset …

Predicting Soil Wind Erosion Potential Under Different Corn Residue Management Scenarios In The Central Great Plains, M. K. Rakkar, Humberto Blanco-Canqui, J. Tatarko

Department of Agronomy and Horticulture: Faculty Publications

Various models and simplified equations are available to predict wind erosion potential. However, their performance can be often site-specific, depending on soil characteristics and agronomic practices, warranting sitespecific model validations. Thus, in this study, we 1) validated the wind erodible fraction (WEF) predictive equations by Fryrear et al. (1994) and López et al. (2007) and 2) estimated the total soil loss with the Singleevent Wind Erosion Evaluation Program (SWEEP) using 3-yr measured data from six experiments located across a precipitation gradient in the central Great Plains. Each site had three corn (Zea mays L.) residue removal treatments: control (no …

Identification Of Quantitative Trait Loci For Plant Height, Crown Diameter, And Plant Biomass In A Pseudo-F2 Population Of Switchgrass, Megan Taylor, Carl-Erik Tornqvist, Xiongwei Zhao, R. W. Doerge, Michael D. Casler, Yiwei Jiang

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass (Panicum virgatum) is a perennial warm-season grass that produces high biomass yield. Identification of mechanisms for genetic regulation of biomass traits has potential to facilitate genetic manipulation of switchgrass for enhancing biomass yield. The objective of this study was to identify quantitative trait loci for biomass-related traits in a pseudo-F₂ population of switchgrass derived from an upland cross with a lowland switchgrass cultivar. Plant height (HT), crown diameter (CD), and plant biomass (PB) were assessed in field studies in 2015 and 2016. Plant height was positively correlated with PB in both years but only correlated with …

Joint Use Of Genome, Pedigree, And Their Interaction With Environment For Predicting The Performance Of Wheat Lines In New Environments, Réka Howard, Daniel Gianola, Osval Montesinos-Lopez, Philomin Juliana, Ravi Singh, Jesse Poland, Sandesh Shrestha, Paulino Pérez-Rodriguez, José Crossa, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

Genome-enabled prediction plays an essential role in wheat breeding because it has the potential to increase the rate of genetic gain relative to traditional phenotypic and pedigree-based selection. Since the performance of wheat lines is highly influenced by environmental stimuli, it is important to accurately model the environment and its interaction with genetic factors in prediction models. Arguably, multi-environmental best linear unbiased prediction (BLUP) may deliver better prediction performance than single-environment genomic BLUP. We evaluated pedigree and genome-based prediction using 35,403 wheat lines from the Global Wheat Breeding Program of the International Maize and Wheat Improvement Center (CIMMYT). We implemented …

A World Of Cobenefits: Solving The Global Nitrogen Challenge, Benjamin Z. Houlton, Maya Almaraz, Viney Aneja, Amy T. Austin, Edith Bai, Kenneth G. Cassman, Jana E. Compton, Eric A. Davidson, Jan Willem Erisman, James N. Galloway, Baojing Gu, Guolin Yao, Luiz A. Martinelli, Kate Scow, William H. Schlesinger, Thomas P. Tomich, Chao Wang, Xin Zhang

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is a critical component of the economy, food security, and planetary health. Many of the world's sustainability targets hinge on global nitrogen solutions, which, in turn, contribute lasting benefits for (i) world hunger; (ii) soil, air, and water quality; (iii) climate change mitigation; and (iv) biodiversity conservation. Balancing the projected rise in agricultural nitrogen demands while achieving these 21st century ideals will require policies to coordinate solutions among technologies, consumer choice, and socioeconomic transformation.

A Two-Amino Acid Difference In The Coat Protein Of Satellite Panicum Mosaic Virus Isolates Is Responsible For Differential Synergistic Interactions With Panicum Mosaic Virus, R. V. Chowda-Reddy, Nathan A. Palmer, Serge Edme, Gautam Sarath, Frank A. Kovacs, Gary Y. Yuen, Robert Mitchell, Satyanarayana Tatineni

Department of Agronomy and Horticulture: Faculty Publications

Panicum mosaic virus (PMV) (genus Panicovirus, family Tombusviridae) and its molecular parasite, Satellite panicum mosaic virus (SPMV), synergistically interact in coinfected proso and pearl millet (Panicum miliaceum L.) plants resulting in a severe symptom phenotype. In this study, we examined synergistic interactions between the isolates of PMV and SPMV by using PMV-NE, PMV85, SPMV-KS, and SPMV-Type as interacting partner viruses in different combinations. Coinfection of proso millet plants by PMV-NE and SPMV-KS elicited severe mosaic, chlorosis, stunting, and eventual plant death compared with moderate mosaic, chlorotic streaks, and stunting by PMV85 and SPMV-Type. In reciprocal combinations, coinfection …

Evaluation Of Spray Pattern Uniformity Using Three Unique Analyses As Impacted By Nozzle, Pressure, And Pulse-Widthmodulation Duty Cycle, Thomas R. Butts, Joe D. Luck, Bradley K. Fritz, W. Clint Hoffmann, Greg R. Kruger

Department of Agronomy and Horticulture: Faculty Publications

BACKGROUND: The increasing popularity of pulse-width modulation (PWM) sprayers requires that application interaction effects on spray pattern uniformity be completely understood to maintain a uniform overlap of spray, thereby reducing crop injury potential andmaximizing coverage on target pests. The objective of this researchwas to determine the impacts of nozzle type (venturi vs. non-venturi), boom pressure, and PWMduty cycle on spray pattern uniformity. Research was conducted using an indoor spray patternator located at the University of Nebraska-Lincoln in Lincoln, NE, USA. Coefficient of variation (CV), root mean square error (RMSE), and average percent error (APE) were used to characterize spray pattern …

Benchmarking Irrigation Water Use In Producer Fields In The Us Central Great Plains, Katherine E. B. Gibson, Justin E. Gibson, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Efficient use of freshwater resources is necessary to balance food production and long-term sustainability of irrigated agricultural systems. Here we developed a framework to benchmark irrigation water use relative to crop yield for individual fields based on site-specific weather and soil. Subsequently, we used the framework to diagnose on-farm irrigation management, in relation to crop production, in maize and soybean producer fields in Nebraska (USA).We found actual irrigation to be similar to estimated irrigation water requirement in about half of the fields (i.e. small water surplus). Remarkably, these fields attained yields similar to fields where actual irrigation exceeded water requirements …

Comparing Infiltration Rates In Soils Managed With Conventional And Alternative Farming Methods: A Meta-Analysis, Andrea D. Basche, Marcia S. Delonge

Department of Agronomy and Horticulture: Faculty Publications

Identifying agricultural practices that enhance water cycling is critical, particularly with increased rainfall variability and greater risks of droughts and floods. Soil infiltration rates offer useful insights to water cycling in farming systems because they affect both yields (through soil water availability) and other ecosystem outcomes (such as pollution and flooding from runoff). For example, conventional agricultural practices that leave soils bare and vulnerable to degradation are believed to limit the capacity of soils to quickly absorb and retain water needed for crop growth. Further, it is widely assumed that farming methods such as no-till and cover crops can improve …

History Of Grass Breeding For Grazing Lands In The Northern Great Plains Of The Usa And Canada, Kenneth P. Vogel, John Hendrickson

Department of Agronomy and Horticulture: Faculty Publications

• In the early 1930s there were millions of acres of extensively degraded grazing lands and abandoned and eroded cropland in the Northern Plains of the United States and Canada.

• Grass breeding and plant materials programs were established by both the US and Canadian governments and cooperating universities to develop revegetation materials.

• Efforts of a small number of research locations and people resulted in grass cultivars or varieties that were used to revegetate and preserve the soil on millions of acres of land.

• This is a brief history of the people, agencies, and universities that developed these …

Do Cover Crops And Corn Residue Removal Affect Soil Thermal Properties?, Michael Sindelar, Humberto Blanco-Canqui, Virginia L. Jin, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Soil thermal properties govern the transport and storage of heat in the soil. How management practices such as crop residue removal and cover crop (CC) use affect these soil properties is not well understood. For example, CCs could provide physical cover and improve soil properties after main crop residue removal and thus ameliorate the negative effects of residue removal on soil thermal properties. We measured changes in soil thermal properties including soil thermal conductivity, thermal diffusivity, volumetric heat capacity, and related properties under corn (Zea mays L.) residue removal with and without winter cereal rye (Secale cereale L.) …

Predicting Longitudinal Traits Derived From High-Throughput Phenomics In Contrasting Environments Using Genomic Legendre Polynomials And B-Splines, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

Recent advancements in phenomics coupled with increased output from sequencing technologies can create the platform needed to rapidly increase abiotic stress tolerance of crops, which increasingly face productivity challenges due to climate change. In particular, high-throughput phenotyping (HTP) enables researchers to generate large-scale data with temporal resolution. Recently, a random regression model (RRM) was used to model a longitudinal rice projected shoot area (PSA) dataset in an optimal growth environment. However, the utility of RRM is still unknown for phenotypic trajectories obtained from stress environments. Here, we sought to apply RRM to forecast the rice PSA in control and water-limited …

Dedicated Bioenergy Crops And Water Erosion, Bharat Sharma Acharya, Humberto Blanco-Canqui, Robert B. Mitchell, Richard Cruse, David Laird

Department of Agronomy and Horticulture: Faculty Publications

Information on the water quality impact of perennial warmseason grasses (WSGs) when grown in marginal lands as dedicated energy crops is limited. We studied how WSGs affected runoff, sediment, and nutrient losses and related near-surface soil properties to those of no-till corn (Zea mays L.) on an eroded soil in southwestern Iowa and a center pivot corner in east-central Nebraska. The experiment at the eroded soil was established in 2012, and treatments included ‘Liberty’ switchgrass (Panicum virgatum L.) and no-till continuous corn. The experiment at the pivot corner was established in 2013 with ‘Liberty’ switchgrass, ‘Shawnee’ switchgrass, low-diversity grass …

Shifting Avian Spatial Regimes In A Changing Climate, Caleb P. Roberts, Craig R. Allen, David G. Angeler, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

In the present era of rapid global change, development of early warnings of ecological regime shifts is a major focus in ecology. Identifying and tracking shifts in spatial regimes is a new approach with potential to enhance understanding of ecological responses to global change. Here, we show strong directional non-stationarity of spatial regimes identified by avian community body mass data. We do this by tracking 46 years of avian spatial regime movement in the North American Great Plains. The northernmost spatial regime boundary moved >590 km northward, and the southernmost boundary moved >260 km northward. Tracking spatial regimes affords decadal …

Rapid Metabolism Increases The Level Of 2,4-D Resistance At High Temperature In Common Waterhemp (Amaranthus Tuberculatus), Chandrima Shyam, Amit J. Jhala, Greg Kruger, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

Common waterhemp emerges throughout the crop growing season in the Midwestern United States, and as a result, the seedlings are exposed to a wide range of temperature regimes. Typically, 2,4-D is used in the Midwest to control winter annual broad-leaf weeds before planting soybean and in an early post-emergence application in corn and sorghum; however, the evolution of 2,4-D-resistant common waterhemp in several Midwestern states may limit the use of 2.4-D for controlling this problem weed. Moreover, temperature is one of the crucial factors affecting weed control efficacy of 2,4-D. This research investigated the effect of temperature on efficacy of …

Recoupling Fire And Grazing Reduces Wildland Fuel Loads On Rangelands, Heath D. Starns, Samuel D. Fuhlendore, R. Dwayne Elmore, Dirac L. Twidwell Jr, Eric T. Thacker, Torre J. Hovick, Barney Luttbeg

Department of Agronomy and Horticulture: Faculty Publications

Fire suppression and exclusion, the historically dominant paradigm of fire management, has resulted in major modifications of fire-dependent ecosystems worldwide. These changes are partially credited with a recent increase in wildfire number and extent, as well as more extreme fire behavior. Fire and herbivory historically interacted, and research has shown that the interaction creates a unique mosaic of vegetation heterogeneity that each disturbance alone does not create. Because fire and grazing have largely been decoupled in modern times, the degree to which the interaction affects fuels and fire regimes has not yet been quantified. We evaluated effects of fire-only and …

Assessment Of Soil Fertility Under Different Land-Use Systems In Dhading District Of Nepal, Sudarshan Kharal, Babu Ram Khanal, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Unscientific land use and cropping techniques have led high soil erosion and degradation of soil quality in the mid-hills of Nepal. To understand the effects of land use systems for selected soil chemical properties in mid-hills, composite soil samples at 0 cm to 20 cm depth were collected from five different land-use systems: Grassland, forest land, upland, lowland, and vegetable farms from Dhading district of Nepal in 2017. Soil samples were analyzed for soil fertility parameters: Soil pH, organic matter (OM), total nitrogen (N), available phosphorus (P), available potassium (K) and its effect due to different land use systems were …

Research Report: Prevalence And Mechanism Of Atrazine Resistance In Waterhemp From Nebraska, Rodrigo Werle, Mithila Jugulam, Greg Kruger, Amaranatha Vennapusa, Felipe Faleco, Bruno Viera, Spencer Samuelson

Department of Agronomy and Horticulture: Faculty Publications

Waterhemp is a troublesome summer annual broadleaf weed species that has evolved resistance to glyphosate and other herbicide sites of action (SOA) in Nebraska, including to groups 2 (i.e., Classic, Pursuit, FirstRate), 4 (i.e., 2, 4-D), 5(i.e., atrazine) and 27 (e.g., Callisto, Laudis). The overall objectives of this study were to

1. Evaluate the eficay of PRE applied atrazine, metribuzin and sulfentrazone to control Nebraska waterhemp populations;
2. Evaluate the efficacy of POST applied atrazine to control Nebraska waterhemp populations; and
3. Determine the mechanism of atrazine resistance in Nebraska waterhemp populations.

Digital Soil Mapping In The Bara District Of Nepal Using Kriging Tool In Arcgis, Dinesh Panday, Bijesh Maharjan, Devraj Chalise, Ram Kumar Shrestha, Bikesh Twanabasu

Department of Agronomy and Horticulture: Faculty Publications

Digital soil mapping has been widely used to develop statistical models of the relationships between environmental variables and soil attributes. This study aimed at determining and mapping the spatial distribution of the variability in soil chemical properties of the agricultural floodplain lands of the Bara district in Nepal. The study was carried out in 23 Village Development Committees with 12,516 ha total area, in the southern part of the Bara district. A total of 109 surface soil samples (0 to 15 cm depth) were collected and analyzed for pH, organic matter (OM), nitrogen (N), phosphorus (P, expressed as P_{2 …}

Overexpression Of The Sorghum Bicolor Sbccoaomt Alters Cell Wall Associated Hydroxycinnamoyl Groups, Hannah M. Tetreault, Erin D. Scully, Tammy Gries, Nathan A. Palmer, Deanna L. Funnell-Harris, Bruce S. Dien, Gautam Sarath, Thomas E. Clemente, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor) is a drought tolerant crop, which is being developed as a bioenergy feedstock. The monolignol biosynthesis pathway is a major focus for altering the abundance and composition of lignin. Caffeoyl coenzyme-A O-methyltransferase (CCoAOMT) is an S-adenosyl methionine (SAM)-dependent O-methyltransferase that methylates caffeoyl-CoA to generate feruloyl-CoA, an intermediate required for the biosynthesis of both G- and S-lignin. SbCCoAOMT was overexpressed to assess the impact of increasing the amount of this enzyme on biomass composition. SbCCoAOMT overexpression increased both soluble and cell wall-bound (esterified) ferulic and sinapic acids, however lignin concentration and its composition …

Elucidating Sorghum Biomass, Nitrogen And Chlorophyll Contents With Spectral And Morphological Traits Derived From Unmanned Aircraft System, Jiating Li, Yeyin Shi, Arun-Narenthiran Veeranampalayam-Sivakumar, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Unmanned aircraft systems (UAS) provide an efficient way to phenotype cropmorphology with spectral traits such as plant height, canopy cover and various vegetation indices (VIs) providing information to elucidate genotypic responses to the environment. In this study, we investigated the potential use of UAS-derived traits to elucidate biomass, nitrogen and chlorophyll content in sorghum under nitrogen stress treatments. A nitrogen stress trial located in Nebraska, USA, contained 24 different sorghum lines, 2 nitrogen treatments and 8 replications, for a total of 384 plots. Morphological and spectral traits including plant height, canopy cover and various VIs were derived from UAS flights …

The Effect Of Ingredient-Specific Calorie Information On Calories Ordered, Christopher R. Gustafson, Eliana Zeballos

Department of Agronomy and Horticulture: Faculty Publications

Providing calorie labeling is a widely used strategy to combat obesity. However, there is little evidence that the availability of calorie information at food-away-from-home establishments has an effect on food choices. Listing calorie information for each ingredient, though, may allow customers to avoid high-calorie items that add little to their enjoyment. Data from a natural experiment were used to compare total calories ordered before and after the provision of per-ingredient versus for build-your-own sandwiches, and per-sandwich for pre-defined sandwiches, at a supermarket sandwich counter. Sandwich order slips from a Lincoln, Neb. supermarket were collected from December 15, 2016 to February …

Phylogenomic Evidence For Ancient Recombination Between Plastid Genomes Of The Cupressus-Juniperus-Xanthocyparis Complex (Cupressaceae), Andan Zhu, Weishu Fan, Robert P. Adams, Jeffrey P. Mower

Department of Agronomy and Horticulture: Faculty Publications

Background: Phylogenetic relationships among Eastern Hemisphere cypresses, Western Hemisphere cypresses, junipers, and their closest relatives are controversial, and generic delimitations have been in flux for the past decade. To address relationships and attempt to produce a more robust classification, we sequenced 11 new plastid genomes (plastomes) from the five variously described genera in this complex (Callitropsis, Cupressus, Hesperocyparis, Juniperus, and Xanthocyparis) and compared them with additional plastomes from diverse members of Cupressaceae.

Results: Phylogenetic analysis of protein-coding genes recovered a topology in which Juniperus is sister to Cupressus, whereas a tree based on …

Mobile Elements Shape Plastome Evolution In Ferns, Tanner A. Robison, Amanda L. Grusz, Paul G. Wolf, Jeffrey P. Mower, Blake D. Fauskee, Karla Sosa, Eric Schuettpelz

Department of Agronomy and Horticulture: Faculty Publications

Plastid genomes display remarkable organizational stability over evolutionary time. From green algae to angiosperms, most plastid genomes are largely collinear, with only a few cases of inversion, gene loss, or, in extremely rare cases, gene addition. These plastome insertions are mostly clade-specific and are typically of nuclear or mitochondrial origin. Here, we expand on these findings and present the first family-level survey of plastome evolution in ferns, revealing a novel suite of dynamic mobile elements. Comparative plastome analyses of the Preridaceae expose several mobile open reading frames that vary in sequence length, insertion site, and configuration among sampled taxa. Even …

Utilizing Random Regression Models For Genomic Prediction Of A Longitudinal Trait Derived From High‐Throughput Phenotyping, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

The accessibility of high‐throughput phenotyping platforms in both the greenhouse and field, as well as the relatively low cost of unmanned aerial vehicles, has provided researchers with an effective means to characterize large populations throughout the growing season. These longitudinal phenotypes can provide important insight into plant development and responses to the environment. Despite the growing use of these new phenotyping approaches in plant breeding, the use of genomic prediction models for longitudinal phenotypes is limited in major crop species. The objective of this study was to demonstrate the utility of random regression (RR) models using Legendre polynomials for genomic …

Identification Of Genomic Regions Contributing To Protein Accumulation In Wheat Under Well-Watered And Water Deficit Growth Conditions, Ibrahim S. Elbasyoni, Sabah M. Morsy, Raghuprakash Kastoori Ramamurthy, Atef M. Nassar

Department of Agronomy and Horticulture: Faculty Publications

Sustaining wheat production under low-input conditions through development and identifying genotypes with enhanced nutritional quality are two current concerns of wheat breeders. Wheat grain total protein content, to no small extent, determines the economic and nutritive value of wheat. Therefore, the objectives of this study are to identify accessions with high and low grain protein content (GPC) under well-watered and water-deficit growth conditions and to locate genomic regions that contribute to GPC accumulation. Spring wheat grains obtained from 2111 accessions that were grown under well-watered and water-deficit conditions were assessed for GPC using near-infrared spectroscopy (NIR). Results indicated significant influences …