Life Sciences Commons^™

Ecological Intensification Of Maize-Kale Systems In Kenya By Biofertilization And Push-Pull Management Shifts Nematode Communities, Agnes Kiriga, Johnnie Van Den Berg, Frank Chidawanyika, Solveig Haukeland, Bianca Greyvenstein, Dennis Beesigamukama, Chrysantus M. Tanga, Sevgan Subramanian, Daniel M. Mutyambai

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Plant-parasitic nematodes (PPNs) pose a persistent threat to vegetable production in Kenya, contributing to reduced yields and soil degradation. The management of PPNs in vegetables in Kenya has largely remained unaddressed. The integration of ecological intensification strategies such as the vegetable integrated push-pull (VIPP) system and the use of black soldier fly frass fertilizer (BSFF) is gaining traction in sustainable agriculture. The aim of this study was to determine the effects of integrating VIPP and BSFF on nematode abundance and diversity in maize-kale cropping systems. Field trials were conducted in two different agroecological zones in Kenya. Field treatments involved plots …

Cross-Domain Antimicrobial Resistance In Poultry Farming: A One Health Assessment Of Antimicrobial Use And Multidrug Resistance In Kiambu County, Kenya, Ann Kangai Munene, Peter Muiruri Mwangi, Lilly Caroline Bebora, Christine Minoo Mbindyo, John Muthini Maingi

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Background and Aim: Antimicrobial resistance (AMR) has emerged as a major One Health threat driven by inappropriate antimicrobial use (AMU) in humans, animals, and the environment. Poultry production is recognized as a key reservoir of antimicrobial-resistant bacteria, yet few studies in Kenya examine AMU and AMR across interconnected human–animal–environment domains. This study assessed AMU patterns among poultry farmers in Kiambu County and characterized phenotypic resistance in Escherichia coli and Enterococcus spp. isolated from humans, chickens, and chicken environments. Materials and Methods: A cross-sectional study was conducted from May to September 2024, involving 102 poultry farms. Farm demographics and AMU data …

Mapping Robusta Coffee (Coffea Canephora) Cropping Systems In Uganda: A Two-Step Pixel And Sub-Pixel Based Approach With Sentinel-2 Data, Getachew Kebede, Bester Tawona Mudereri, Onisimo Mutanga, Tobias Landmann, John Odindi, Natacha Motisi, Fabrice Pinard, Henri E.Z. Tonnang, Elfatih M. Abdel-Rahman

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Coffee is a highly valued commodity and a widely consumed beverage, playing an important role in global trade. However, coffee farming landscapes are increasing transitioning into smaller-scale agricultural setups. This transformation highlights the critical need for accurate classification and mapping of coffee cropping systems (CS), especially in countries like Uganda, where dense vegetation and complex terrain present substantial challenges to traditional land survey methods. Moreover, understanding the spatial distribution of Robusta coffee (Coffea canephora) CS is essential for developing site-specific management strategies, guiding extension services, and informing evidence-based policy decisions. To address this gap, the present study aimed to enhance …

A Systematic Review And Meta-Analysis Of Mosquito Arboviral Infections Detected Through Xenosurveillance In Africa: A Focus On West Nile, Rift Valley Fever, And Chikungunya Virus Infections, Mxolisi Mgongoma, Armanda Bastos, Abel Ramoelo, David P. Tchouassi

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This systematic review and meta-analysis comprehensively evaluated the mosquito arboviral infections with West Nile virus (WNV), Rift Valley fever virus (RVFV), and chikungunya virus (CHIV), detected through xenosurveillance, reported in mosquito vectors across Africa in the last 25 years (2000–2024). The study analysed xenosurveillance data from 45 studies conducted with over 115,000 mosquito specimens, and incorporated moderator analyses to assess the influence of trap type, seasonality, regional location, settlement type, and screening methods on virus detection rates. The estimated pooled prevalence of WNV was 1.18% (95% CI: 0.60–3.43%), with higher rates observed in Culex quinquefasciatus and Culex pipiens, particularly in …

Optimal Control Analysis Of Malaria Transmission In The Presence Of Insecticide Resistance And Climate Variability In Kenya, Lorna Chepkemoi, Titus Okello Orwa, Samuel Mwalili, Rachel Waema Mbogo, Steeven Belvinos Affognon, Daisy Salifu, Henri E.Z. Tonnang

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Malaria remains a major public health concern in Kenya, where changing climatic conditions, insecticide resistance, and mosquito behavioral adaptations continue to challenge control efforts. This study develops and analyzes a climate-sensitive malaria transmission model that incorporates mosquito behavior, insecticide resistance, and vector–human ecological dynamics to identify optimal control strategies for the Kenyan context. The model’s well-posedness was established, the basic reproduction number (R0) computed using the next-generation matrix method, and the stability of equilibrium points assessed. Spatial analysis of R0 was performed using temperature and rainfall raster data to map transmission risk across Kenya under varying insecticide use scenarios. Results …

Editorial: Sustainable Management Of Tuta Absoluta, Ahmad A. Omar, Inusa Jacob Ajene, Hafiz Muhammad Usman Aslam, Fathiya Mbarak Khamis

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The tomato leafminer, Tuta absoluta (Meyrick), remains one of the most devastating global threats to tomato production. Its rapid invasion across continents, exceptional reproductive potential, cryptic foliar-mining behavior, and accelerated evolution of insecticide resistance have significantly complicated management efforts. The persistent failure of conventional chemical strategies underscores an urgent need for integrated solutions that combine molecular innovation, ecological engineering, and rigorous resistance monitoring. This editorial synthesizes key insights from recent contributions by Ullah et al., Bavithra et al., and Adams et al., which collectively outline a transformative, multi-dimensional framework for sustainable T. absoluta suppression.

Insect Farming Innovations: A Pathway To Self-Reliance In Agrifood Systems In Africa, Zewdu Abro, Menale Kassie, Dennis Beesigamukama, Shaphan Yong Chia, Ermias Engida Legesse, Subramanian Sevgan, Julius Ecuru, Chrysantus M. Tanga

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Insect farming as an innovative approach to transforming agrifood systems

Insect farming has emerged as a transformative and sustainable approach to addressing the interconnected challenges of food insecurity, environmental degradation, and economic vulnerability in Africa’s agrifood systems. By converting organic waste into high-value products (protein-rich feed, frass fertilizers, and bioactive compounds), insect farming offers an efficient circular economy solution that bridges nutrition, livelihoods, and ecological resilience (Lalander et al. 2025; Mei et al. 2024; Barragán-Fonseca et al. 2022; Xia et al. 2021; Van Huis 2020; Barragán-Fonseca et al. 2017; Van Huis and Oonincx 2017; Yi et al. 2014). Almost all …

First Record Of Arrhenophagus Chionaspidis Aurivillius (Hymenoptera: Encyrtidae) Parasitizing White Mango Scale In Kenya: Its Potential Distribution And Prospects In Biocontrol, Francis Obala, Abdelmutalab G.A. Azrag, Rehemah Gwokyalya, Shepard Ndlela, Ingo Grass, Georg Petschenka, Sunday Ekesi, Samira A. Mohamed

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The white mango scale, Aulacaspis tubercularis Newstead (Hemiptera: Diaspididae), is one of the most destructive pests of mango worldwide. Its current management in most of the invaded areas largely depends on the intensive use of chemical insecticides. Biological control using parasitoids represents one of the most effective and environmentally sustainable management options. However, in Kenya, one of Africa's leading mango-producing countries, no effective parasitoid species associated with A. tubercularis had previously been recorded. This study aimed to identify encyrtid parasitoids associated with A. tubercularis in Kenya, and to assess how bioclimatic factors influence their occurrence and habitat suitability. Mango leaves …

Reinterpreting Stochastic Optimal Control Under Ecological Uncertainty: Inferring Decision Urgency From Vegetation Biomass Dynamics, Komi Mensah Agboka, Tobias Landmann, Elfatih M. Abdel-Rahman

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Stochastic optimal control provides a rigorous framework for systems subject to uncertainty, yet its operational use in ecological crisis contexts remains limited by interpretability. We reinterpreted a stochastic control formulation in which selected parameters emerged as indicators of decision urgency rather than normative preferences. Vegetation biomass was modeled as a stochastic stock subject to nonlinear loss driven by feeding pressure (e.g., desert locust activity) and multiplicative noise, with uncertainty represented by a time-varying volatility term that integrated extreme rainfall anomalies and conflict-related disruption. Rather than prescribing an optimal policy, we inverted the closed-form solution of the control problem to infer …

Impact Of Environmental Microparticles On Insect Olfaction, Steve B.S. Baleba, Danube K.N. Wandji, Yves H. Tchiechoua, Komi Mensah Agboka, Iman B. Hassaballa, Victor O. Omondi, Beatrice T. Nganso, Saliou Niassy, Souleymane Diallo, Merid N. Getahun

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Terrestrial insects underpin key ecosystem services, including pollination, herbivory regulation, decomposition, nutrient cycling, and disease control. These functions depend on chemical communication that guides insects to food, mates, hosts, shelters, and oviposition sites while helping them avoid threats. Environmental microparticles, such as micro- and nanoplastics, tyre wear particles, soot, mineral dust, and agricultural residues, are now widespread across air, soil, vegetation, and indoor environments, exposing insects through contact, deposition, and ingestion. Growing evidence shows that these particles disrupt insect olfaction by adsorbing volatile compounds, blocking antennal sensilla, and interfering with receptor and neuronal processes. These disruptions impair foraging, mating, oviposition, …

Comparing Autodissemination, Soil Inoculation And Their Combination As Modes Of Application Of Metarhizium Anisopliae Icipe 69 On The Melon Fly Infesting Courgette In Kenya Under Semi-Field Conditions, Rehemah Gwokyalya, Thomas Dubois, Susan K. Onsongo, Joseph A. Odhiambo, Komivi Senyo Akutse, Samira A. Mohamed

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The melon fly Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) is a significant pest of cucurbit crops, responsible for substantial yield losses and economic damage across Asia and Sub-Saharan Africa. Control of this pest currently relies heavily on synthetic insecticides, which are associated with environmental and human health risks, and contribute to the development of insecticide resistance. Entomopathogenic fungi offer a promising eco-friendly alternative, yet effectiveness of delivery methods remains a critical limitation in their widespread adoption. This study evaluated the efficacy of three application modes of Metarhizium anisopliae (Mechnikov) Sorokin (Hypocreales: Clavicipitaceae) isolate ICIPE 69, autodissemination devices (AD), soil inoculation (soil), …

Sustainable Control Of Pests And Striga Weed Through Intensified Push-Pull Technology And Frass-Based Soil Amendments, Evanson R. Omuse, Honest Machekano, Daniel M. Mutyambai, Dennis Beesigamukama, Chrysantus M. Tanga, Samwel O. Ogaji, Sevgan Subramanian, Frank Chidawanyika

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Crop productivity under African smallholder farming systems is faced by numerous challenges, including insect pests, weeds, low soil fertility, and climate change. Sustainable intensification, such as push-pull (PP) technology and vegetable-integrated push-pull (VIPP) technology, coupled with soil organic amendments such as black soldier fly frass fertilizer (BSFFF), can address these agricultural constraints. We investigated the individual and synergistic effects of cropping systems comprising two crops [maize (DK777 variety) and cowpea (KVU27 variety)] and agroecological strategies (PP, VIPP and BSFFF) on the management of pests and Striga weeds and crop productivity in three different geographical areas. Diversified cropping systems (PP and …

Impacts Of Multitrophic Interactions In Diversified Cropping Systems For Sustainable Management Of Spodoptera Frugiperda, Abdul A. Jalloh, Abdullahi Ahmed Yusuf, Fathiya M. Khamis, Subramanian Sevgan, Daniel Munyao Mutyambai

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Background: Rapid population growth has made the sustainable intensification of agricultural productivity essential for meeting global food demands while maintaining environmental integrity and biodiversity. Diversified cropping systems, such as intercropping, push-pull systems, and cover cropping, are recognized as agroecological strategies to enhance plant growth and protection, and suppress major pests, including the fall armyworm (FAW, Spodoptera frugiperda). These systems influence complex above-and-below ground interactions among herbivorous pests, plants, soil microbes, and their natural enemies. Aims: This review synthesizes current knowledge on how diversified cropping systems shape multitrophic interactions that affect soil health, plant performance, S. frugiperda suppression, and biocontrol within …

Behavioral And Physiological Insights Into Cross-Stage Interactions In The Desert Locust, Benjamin Fürstenau, Vincent O. Nyasembe, Hosea O. Mokaya, Hillary K. Kirwa, Angel Guerrero, Baldwyn Torto

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Gregarious desert locusts produce stage-specific pheromones that facilitate cohesive behavior in juveniles and synchronize maturation and mating in sexually mature adults. During locust outbreaks, merging populations result in cross-stage interactions, yet their impact on locust biology remains poorly understood. This study tested the hypothesis that cross-stage interactions influence juvenile cohesion and physiological traits. Using behavioral assays and gas chromatography-mass spectrometry, we examined short- and long-term interactions between juvenile and adult desert locusts. In short-term (24 h) cage assays, the presence of adults did not significantly affect grouping behavior in gregarious 3rd instar nymphs, as measured by the mean distance between …

Infochemicals For The Management Of Thysanoptera, Nanadagopal Bakthavatsalam, Kesavan Subaharan, Anjenappa Raghavendra, Sevgan Subramaniam

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Infochemicals, classified as pheromone, kairomone, allomone, synomone and apneumone, play significant role in insect communication. Unlike Lepidoptera, Thysanoptera utilize male produced aggregation pheromone for their communication. Neryl (S)-2-methybutanoate, (R) lavandulyl acetate, (+) R-lavandulyl 3 methyl-butenoate, dodecyl acetate and tetradecyl acetate are the compounds identified as aggregation pheromones for several species. Pheromone lures placed in blue or yellow or white sticky traps are effective in capturing adults of Frankliniella, Thrips, Megalurothips and Odonothrips. Decyl acetate and dodecyl acetate along with other volatile chemicals have been identified as alarm pheromones for many Thysanoptera. Push-Pull technology using alarm pheromone and aggregation pheromone or …

When Thermal Risk Indices Work And When They Don't: A Case Study Of Two Maize Insect Pests, Komi Mensah Agboka, Frank Thomas Ndjomatchoua, Luca Rossini, Ritter A. Guimapi, Elfatih M. Abdel-Rahman

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The biological life cycle of terrestrial arthropods, using temperature as the primary driving factor has a large interest for insect pests in agriculture, forestry, urban ecosystems, as constitutes the basics for the development of mathematical models for decision making. A recent study proposed a physiologically-based risk index (RI) which finds large applications in the definition of risk maps; however, further case studies are needed to better explore its strengths and limitations. This study aims to extend this knowledge by presenting an application of the RI on two economically significant pests: the fall armyworm Spodoptera frugiperda and the stem borer Busseola …

Maximizing Horizontal Transmission Through Mating: Increased Mating Frequency And Mating Competitiveness Associated With Microsporidia Mb-Infected Anopheles Arabiensis Males, Tracy Maina, Aclaine Shisia, Joseph Gichuhi, Joel L. Bargul, Jeremy K. Herren, Tullu Bukhari

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Background: Microsporidia MB is a naturally occurring symbiont in Anopheles arabiensis mosquitoes that inhibits the development of Plasmodium. It is transmitted both vertically and horizontally, enabling its spread within mosquito populations. Currently, mating is the only known mechanism for horizontal transmission. Understanding the factors that influence Microsporidia MB transmission during mating is crucial for developing a malaria transmission-blocking strategy based on this symbiont. Methods: The effect of mosquito age on Microsporidia MB transmission was determined through mating bioassays between infected and uninfected Anopheles arabiensis males and females in three age groups: 3–4 days, 7–8 days, and 10–11 days. Mating bioassays …

De Novo Genome Assembly, Annotation, And Characterization Of Chemosensory Genes In The Camel Ked (Hippobosca Camelina), Fredrick Kebaso, Souleymane Diallo, Caleb Kibet, Suhaila Hashim, John Mark O. Makwatta, Merid Getahun, Dennis Getange, Jean Baka Domelevo Entfellner, Daniel Masiga

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Background: Hippobosca camelina (camel ked) is an obligate hematophagous ectoparasite that infests camels. Hematophagy inflicts painful bites leading to myiasis, anemia and pathogen transmission such as Candidatus Anaplasma camelii. A genome assembly for this biting flies is currently unavailable, limiting understanding of its genetics, including the chemosensory system.

Results: The assembly size for Hippobosca camelina female is 135.6 Mb with 17.08% repeated regions, an N50 of 1.2 Mb, a total of 2,182 contigs, a GC content of 33.5% and compleasm (Busco) completeness of 95.38% with the diptera_odb10 lineage (S:95.83%, 3148, D:0.30%, 10, F:0.30%, 10, I:0.00%, 0, M:3.56%, 117, N:3285) …

Techno-Functional Properties Of Protein-Rich Flours From Different Species Of Edible Insects As Affected By Drying Methods, K. B.Yisa Njowe, K. G. Duodu, M. N. Emmambux

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Food-based ingredients from insects can circumvent the challenging disgust factor of consuming edible insects as food. The functional properties of dried edible insect flour could guide the use of edible insects as food ingredients in familiar foods. This study investigated the effect of freeze drying, oven drying, sun drying, solar cabinet drying, boiled before either sun drying or solar cabinet drying techniques on the functional properties of protein-rich flours from edible grasshoppers, crickets, and caterpillars. The Nitrogen solubility indices (NSI), water solubility indices (WSI) and β-sheets for all boiled solar-dried grasshopper, cricket, and caterpillar protein concentrates decreased compared to the …

The Dissemination Potential Of Microsporidia Mb In Anopheles Arabiensis Mosquitoes Is Modulated By Temperature, Fidel Gabriel Otieno, Priscille Barreaux, Affognon Steeven Belvinos, Edward Edmond Makhulu, Thomas Ogao Onchuru, Anne Wambui Wairimu, Stancy Mandere Omboye, Cynthia Nyambura King’Ori, Bonoukpoè Mawuko Sokame, Anthony Kebira Nyamache, Jeremy Keith Herren

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Microsporidia MB, a vertically transmitted endosymbiont of Anopheles mosquitoes, shows strong potential as a malaria control agent due to its ability to inhibit Plasmodium development within the mosquito host. To support its deployment in malaria transmission reduction strategies, it is critical to understand how environmental factors, particularly temperature, influence its infection dynamics. In this study, we investigated the impact of four temperature regimes (22 °C, 27 °C, 32 °C, and 37 °C) on Microsporidia MB prevalence and infection intensity by rearing mosquito larvae under controlled laboratory conditions. Our results demonstrate that elevated temperatures, especially 32 °C, significantly enhance both larval …

A Randomized Controlled Trial Combining House Screening And Insecticide-Treated Nets Reduces Malaria Transmission In Northwestern Ethiopia, Aklilu K. Belay, Abebe Asale, Catherine L. Sole, Abdullahi A. Yusuf, Baldwyn Torto, Zewdu Abro, Menale Kassie, Clifford M. Mutero, David P. Tchouassi

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House screening (HS) of doors, eaves, and windows using wire-mesh has demonstrated potential in the integrated vector management of malaria. However, limited epidemiological data are available to guide its implementation across different ecological settings. In a 16-month randomized controlled trial (follow-up period) conducted across three agroecological areas (dry mountain, plateau highland, and semi-arid) in Jabi Tehnan district, northwestern Ethiopia, treatment houses were equipped with HS combined with insecticide-treated nets (ITNs), while control houses received ITNs only. The intervention led to a significant 2.3-fold reduction in indoor malaria vector density, the primary entomologic outcome, largely influenced by An. gambiae s.l. mosquitoes. …

Microsporidian Infection Of Mosquito Larvae Changes The Host-Associated Microbiome Towards The Synthesis Of Antimicrobial Factors, Artur Trzebny, Abigail D. Taylor, Jeremy K. Herren, Johanna K. Björkroth, Sylwia Jedut, Miroslawa Dabert

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Background: Microsporidians (Microsporidia) are a group of obligate intracellular parasites that commonly infect mosquitoes. Recently, it has been shown that infection by these parasites can alter the composition and functionality of the mosquito-associated microbiome. The host-associated microbiome of the mosquito can play a pivotal role in various physiological processes of this host, including its vector competence for pathogens. Thus, understanding how microsporidians shape the mosquito microbiome may be crucial for elucidating interactions between these parasites and their mosquito hosts, which are also vectors for other parasites and pathogens. Methods: The effects of microsporidian infection on the microbiome structure and functionality …

Regional Variation And Epidemiological Insights In Malaria Underestimation In Cameroon, Sarafa A. Iyaniwura, Qing Han, Ngem Bede Yong, Ghislain Rutayisire, Agnes Adom-Konadu, Okwen Patrick Mbah, David Poumo Tchouassi, Kingsley Badu, Jude Dzevela Kong

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Background: Despite significant global effort to control and eradicate malaria, many cases and deaths are still reported yearly. These efforts are hindered by several factors, including the severe underestimation of cases and deaths, especially in Africa. Methods: We used a mathematical model, incorporating the underestimation of cases and seasonality in mosquito biting rate, to study the malaria dynamics in Cameroon. Using a Bayesian inference framework, we calibrated our model to the monthly reported malaria cases in ten regions of Cameroon from 2019 to 2021 to quantify the underestimation of cases and estimate other important epidemiological parameters. We performed Hierarchical Clustering …

Pretreatment Methods For Organic Waste Management As Feed For Black Soldier Fly, Mube K. Hervé, Gatchueng F. Lionel, Ndomou C.H. Serge, Dzepe Daniel, Shaphan Y. Chia, Kana Jean Raphael, Kenfack Augustave, Djouaka Rousseau, Sali A. Ndindeng

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Amidst escalating global urbanization and population growth, revolutionizing food systems and redefining waste management strategies has become essential. This study contributes to protein resource diversification in animal feed and offers a sustainable solution for organic waste management using black soldier fly larvae (BSFL) in Bafia, Cameroon. A survey characterized waste production in restaurants and households to assess quantity and quality. Collected organic wastes underwent two pre-treatments: heating, (60°C) and fermentation (4 days), while another batch was used fresh as a feeding substrate for BSFL, in contrast to a broiler starter diet over a 17-day larval feeding experimental period. Various parameters …

Integrating Metabolomics And Physicochemical Analysis In Black Soldier Fly Bioconversion Of Schizochytrium Residue: A Pathway To High-Value Ω-3 Products, Yaru Zhang, Jing Liu, Yan Xiao, He Yang, Yanzhou Zhang, Chrysantus Mbi Tanga, Dawei Huang, Jinhua Xiao

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The global microalgae processing industry generates substantial residues rich in ω-3 fatty acids, presenting both a waste management challenge and an untapped resource for innovative food biotechnology. This study investigated the potential of bioconversion of Schizochytrium residue (SCR) by black soldier fly larvae (BSFL) to enrich ω-3 fatty acids and evaluated the industrial applicability of the resulting BSFL products. SCR supplementation enhanced larval growth performance (7.5 % increased weight, 19.9 % higher bioconversion rate) and protein quality, while enabling exclusive accumulation of EPA and DHA in BSFL. The total ω-3 content increased from 1.18 % to 17.37 %, achieving a …

Insect Frass Fertilizer Upregulates Maize Defence Genes And Resistance Against An Invasive Herbivore Pest, Daniel Munyao Mutyambai, Johnstone Mutiso Mutua, Abdul A. Jalloh, Dennis Beesigamukama, Andre Kessler, Sevgan Subramanian, Thomas Dubois, Sunday Ekesi, Chrysantus M. Tanga

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The black soldier fly frass fertilizer (BSFFF) has gained global attention as a multipurpose input for soil fertilization and pest and disease management. However, there are limited studies that have examined its effects on insect pest resistance and the underlying mechanisms. We investigated the impact of amending soil with BSFFF on maize growth, defense gene expression and resistance to a polyphagous insect herbivore, Spodoptera frugiperda (Lepidoptera: Noctuidae) through larval feeding assay. Maize growth was evaluated by measuring plant height, chlorophyll concentration, and biomass accumulation in soils amended with BSFFF, synthetic fertilizers (Di-ammonium phosphate and Calcium ammonium nitrate) and unfertilized soils …

Fusarium Equiseti Pathogen Of Potato In Kenya And Its Potential In Vitro Biocontrol Using Fungal Endophytes, Marie Cecile Muhorakeye, Fathiya M. Khamis, Everlyne Samita Namikoye, Komivi S. Akutse

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Accurate pathogen diagnosis is fundamental for effective crop disease management. In Kenya, a pathogen causing significant damage in potato farms was initially misidentified as Phytophthora infestans. This study aimed to correctly identify this pathogen and explore initial control measures using a polyphasic approach. The methodology integrated morphological observation, pathogenicity testing, and molecular analysis using ITS and EF1-α gene sequencing. The results confirmed the pathogen’s identity as Fusarium equiseti, with morphological features consistent with this species and molecular sequencing showing 99.6% identity to reference strains. This is the first official report of Fusarium equiseti as a potato pathogen in Kenya. Furthermore, …

Spatio-Temporal Dynamics Of Malaria Vector Niche Overlaps In Africa, Eric Ali Ibrahim, John Odindi, Mark Wamalwa, Henri E.Z. Tonnang

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Malaria remains a significant public health challenge in sub-Saharan Africa, with transmission heightened by the dynamics of primary and secondary mosquitoes infected with Plasmodium parasites. Regions where both vector types co-exist face heightened likelihood of intensified malaria transmission. Hence, understanding vectors' ecological interactions, especially their niche overlaps in geographic or environmental space, is crucial for targeted malaria control and elimination strategies. We employed a dynamic cellular automata (CA) model to map niche overlaps among primary (Anopheles gambiae complex, An. funestus group) and secondary (An. pharoensis, An. coustani) malaria vectors across African, using open-access environmental and vector occurrence datasets sourced from …

Spatial Distribution Of Microsporidia Mb Along Clinal Gradient And The Impact Of Its Infection On Pyrethroid Resistance In Anopheles Gambiae S.L. Mosquitoes From Nigeria And Niger Republic, Lamine M. Moustapha, Muhammad M. Mukhtar, Abdoul Nasser H. Sanda, Shuaibu Adamu, Yusuf Y. Aliyu, Hadizat K. Einoi, Maryam U. Maigari, Peter C. Okeke, David E. Nwele, Abiodun Obembe, Udoka C. Nwangwu, Jeremy K. Herren, Sulaiman S. Ibrahim

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Microsporidia MB (MB), a promising biological control agent, suppresses Plasmodium falciparum transmission in Anopheles mosquitoes. This study examined the spatial distribution of MB infection in natural populations of An. gambiae s.l. mosquitoes collected in Nigeria and Niger Republic, and its association with insecticide susceptibility in the mosquitoes. Microsporidia MB has wide geographic distribution across Nigeria and Niger Republic. The overall prevalence of MB in F0 mosquitoes was 12.25% (95% CI: 7.76–16.75%); 25 mosquitoes out of 204 were positive. Geographic variation was observed, with a higher prevalence (5/15 mosquitoes) in Ebonyi State (33.33%, CI: 9.48–57.19%, Fisher’s exact test, p = 0.008). …

Maize Yield Stability Under Organic And Conventional Farming Systems In Sub-Humid Agro-Ecozones Of Central Kenya, Milka Kiboi, David Bautze, Felix Matheri, Amritbir Riar, Andreas Fliessbach

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Maize (Zea mays L.) is the main staple crop and is important to the food security and livelihoods of people globally. We evaluated the effects of organic and conventional farming systems on maize grain yield and temporal yield stability under different external input levels in a long-term experiment (2007–2022) at two sites in Kenya. We implemented four farming systems: organic high (Org-High), conventional high (Conv-High), organic low (Org-Low), and conventional low (Conv-Low) in a randomized complete block design. Growth indicators for maize, height, stem diameter, and grain yield were higher in high input systems, but grain yield stability was not. …