Life Sciences Commons^™

Influence Of Sodium Chloride, Calcium, Moisture, And Ph On The Structure And Functionality Of Nonfat Directly Acidified Mozzarella Cheese, Brian M. Paulson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experiment A explored the influence of sodium on direct acid, nonfat Mozzarella cheese. Cheeses with differing salt levels were obtained by varying dry salt applications (none, 0.5%, and 1.0% NaCl w/w) and hot brine stretching (0%, 5%, and 10% NaCl wt/v). Salt application and salt content influenced cheese moisture, meltability, expressible serum, micro- and ultra-structure, and color. Moisture was highest when cheese was salted before stretching (P = 0.03). Melt was lowest in cheeses that were unsalted (P = 0.05). Cheeses stretched in salt brine had < 1% of the amount of expressible serum found in unsalted cheese (P < 0.0001). Unsalted cheeses had a more open structure with pockets of serum distributed throughout the protein matrix giving it an opaque, white appearance. Salted cheeses had a more homogeneous protein matrix lacking light scattering surfaces, resulting in a translucent cheese. Neither salt concentration nor method of salting affected the calcium content of the cheeses (P > 0.05).

Experiment B explored the influence of calcium, moisture, and …

Comparative Effects Of Sodium Levulinate And Sodium Lactate On Microbial Growth, Color, And Thiobarbituric Acid (Tba) Values Of Fresh Pork And Turkey Sausages During Storage, Mihir N. Vasavada

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study compared the effects of 1.4 or 2.7% sodium levulinate or sodium lactate on aerobic plate count (APC), color, pH, and TBA value of fresh pork and turkey sausage. Both sodium lactate and levulinate inhibited growth of aerobic microorganisms during storage, compared to controls. Bacteriostatic effects of sodium lactate were dose dependent, wherein 2.7% lactate was significantly more antimicrobial than 1.4% lactate. This was not the case for sodium levulinate, where 1.4% sodium levulinate was as inhibitory to microbial growth as 2.7% sodium levulinate. Additionally, 1.4% sodium levulinate was as inhibitory to microbial growth as the higher level (2.7%) …

Cultivation Of Mushroom Mycelia Using Whey Products As A Growth Substrate, Boyd S. Inglet

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As part of a project designed to utilize common dairy waste products profitably, reconstituted dry whey permeate and delactosed whey were tested as growth substrates for mycelia of the edible mushroom Lentinus edodes. This mushroom was chosen because it is possible to profitably cultivate it due to its popular culinary appeal and perceived medical benefits.

Growth experiments were performed in petri dishes containing either reconstituted dry whey permeate or delactosed whey as a growth substrate, and the measured response was the size of the growing mycelia colony. When reconstituted dry whey permeate was utilized as a growth substrate, the …

Casein Supramolecules: Structure And Coagulation Properties, Bonney S. Oommen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The changes in quaternary structure of casein supramolecules with various physical and chemical treatments were studied using transmission electron microscopy, and a model to account for the changes is put forth. The effects of casein structure on coagulation properties were also studied. The sample preparation for transmission electron microscopy involved physical methods of fixation and flash freeing to preserve the structure of caseins in the sample.

The structure of caseins in sodium and calcium caseinate varied with sodium caseinate not exhibiting any spherical structure as opposed to the spherical structure seen in calcium caseinate, non-fat dried milk and native milk. …

Nutrition And Bone Density In Children With Cystic Fibrosis, Joanna K. Davidson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of these studies was to further research on bone density in children with cystic fibrosis, particularly as it pertains to nutritional parameters and care. The first paper presented a comparison of a group of 50 children with cystic fibrosis to a group of 32 control children. There were no significant differences between the groups in any of the pertinent bone density measurements. Serum 25(OH) vitamin D was positively correlated with spine density z score in the cystic fibrosis group.

The second paper, incorporating all of the information obtained from the first paper, describes an intervention study with the …

Hormone Mediated Transport Of Calcium And Phosphate In Polarized Epithelial Cells, Tremaine M. Sterling

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of 1,25(OH)₂D₃, PTH and 25(OH)D₃ on phosphate or calcium uptake were studied in cultured, adherent chick enterocytes over a period of 10 min after hormone addition. Time course studies of cells treated with 130 pM 1,25(OH)₂D₃ showed an increase in ³²P uptake as early as 3 min. Similar studies with 65 pM bPTH(l1-34) resulted in an increase in ⁴⁵Ca uptake only if the cells had been cultured in serum. (OH)D₃, which is not firmly established as an active metabolite of vitamin D, was shown to increase …

Greenstrip Establishment And Management In The Intermountain West, Brenda Kristine Younkin-Kury

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greenstrips were established at two sites in Utah to determine if seeded, grazed cool-season, perennial grasses would change fire behavior characteristics in areas currently dominated by Bromus tectorum. Frequency data were collected for both grazed and ungrazed seeded species and resident weed species. Moderate spring grazing did not negatively impact the establishment of seeded species at Camp Williams. Grazing at Promontory Point decreased Agropyron desertorum frequency and increased the frequency of Pascopyrum smithii. Biomass data collected for grazed and ungrazed treatments in both years indicated that moderate spring or winter grazing the first two years of establishment did …

Cougar Exploitation Levels In Utah: Implications For Demographic Structure, Metapopulation Dynamics, And Population Recovery, David C. Stoner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Presently, eleven western states and two Canadian provinces utilize sport hunting as the primary mechanism for managing cougar (Puma concolor) populations. However, the impacts of sustained harvest on population dynamics and demographic structure arc not well understood. Additionally, the lack of cost-effective enumeration techniques and strongly conflicting societal values complicate effective management of this species. Given these concerns, the primary goals of this study were (I) to determine the effects of sustained harvest on cougar populations, and (2) estimate the level and extent of cougar harvest statewide.

I monitored cougar populations on Monroe Mountain in south-central Utah, and …

Remote Sensing Of Sagebrush Community Structural Patterns Across Scales, Lisa A. Langs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Throughout the Intermountain West there has been a substantial reduction in both the quantity and quality of sagebrush ecosystems. To assist current range management objectives, numerous efforts have been made to classify and map sagebrush communities using remotely sensed data. However, the amount of detail provided by these broad-scale mapping projects is often limited. This research evaluated the ability of a suite of airborne and satellite imagery to detect sagebrush community structural attributes, specifically percent canopy cover, live cover, density, size-vigor, and spatial arrangement of shrubs. Field data was collected at Camp Williams National Guard Training Facility near Bluffdale, Utah, …

Quantifying Losses Of Understory Forage In Aspen Stands On The Dixie And Fishlake National Forests, Barton R. Stam

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The West has lost up to 60% of its historic aspen stands over the last century, probably as a result of the successional tendency of aspen to be replaced by coniferous species in the absence of periodic fires. One of several major impacts of this change is the loss of understory forage as conifer canopy cover increases. I measured understory biomass in aspen stands ranging from 0% to 81 % absolute conifer cover in the canopy and found that understory production declines exponentially as conifers replace aspen. I also did an economic analysis to determine the value of the forage …

Role Of Iron In The Mechanism Of Asbestos-Induced Apoptosis In Human Lung And Pleural Target Cells, Aleksander Baldys

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Occupational exposure to asbestos has been associated with increased incidence of pulmonary interstitial fibrosis, mesothelioma of the pleura, and bronchogenic carcinoma. Although the mechanism by which asbestos causes cancer remains unknown, iron associated with asbestos is thought to play a role in the pathogenic effects of fibers.

The aim of this research was to examine and compare the asbestos-induced signaling phenomena in relevant human lung and pleural target cells, and to determine the role of iron from asbestos fibers in these events. Exposure of human airway epithelial (A549) cells, human pleural mesothelial (MET5A) cells, and normal human small airway epithelial …

Host Shifts In Phytophagous Insects: A Mathematical Modeling Perspective, Meenakshi Venkataraman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I explored changes in predator-prey system stability with the addition of spatial heterogeneity in prey density dependent mortality. The results indicated that this addition led to greater stability for all prey spatial distributions if prey was aggregated or loosely aggregated. These results indicated prey spatial distribution was critical to population stability. I next explored whether predator emigration under the above conditions would lead to a host shift. I used Chesson and Murdoch's templates of host density independent (HDI) aggregation and host density dependent (HDD) aggregation in predators as the basis for my metapopulation models. I varied prey density dependent mortality …

Nest Location And Nest Recognition In Two Solitary Bee Species Osmia Lignaria Say And Megachile Rotundata (F.) (Hymenoptera: Megachilidae), Christelle Guédot

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The visual and olfactory cues used in short-range orientation, specifically nest location and nest recognition, were studied in two solitary bee species Osmia lignaria Say and Megachile rotundata (F.) Osmia lignaria, the blue orchard bee, is an important pollinator of orchard crops, such as apples, cherries, and almonds; and M. rotundata, the alfalfa leafcutting bee, is used in commercial pollination of alfalfa. The general objective was to better understand how these two species locate their nests and how improving nest location could benefit crop pollination.

The use of proximal visual landmarks at the nesting site was investigated with …

Influence Of Storage Duration, Temperature, And Oxygen On Quality Of Stored Dehydrated Foods, Omima Ali Darag

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was undertaken to determine the effect of oxygen absorber packets (OAP) (AGELESS Z.300 E) in improving the shelf-life of selected dried food products, including dried potato pearls, dried sliced apple, dried carrot, white rice, nonfat dry milk, all purpose flour, rolled oats, and wheat. Items were stored at four different temperatures (-20, 10, 21, and 29.5°C) over a period of 24 months with observations at 6-month intervals. Hunter colorimeter, thiobarbituric acid reactive substance (TBARS) germination rate, gluten weight, and volume were parameters used as quality indicators of various dried foods.

Statistical analysis indicated a significant time and temperature …

Breeding Bird Communities Of Major Mainland Rivers Of Southeastern Alaska, Jim A. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Because of the scarcity of information for bird communities at the major mainland rivers of southeastern Alaska, the main objective of this study was to provide baseline information including distribution, status, and habitat associations of breeding birds.

I conducted a meta-analysis of all known reports (including the current study) conducted at major mainland rivers during the breeding season. I described bird species composition, distribution, abundance estimates, status, habitat associations, and guild membership for all birds recorded at 11 major mainland rivers. Based on incidental observations, 170 species were recorded by all studies. Of these, 134 species were known or suspected …

Catastrophic Wildfire Hazard Assessment In Pinyon-Juniper Woodlands Utilizing A Managerial Paradigm, Benjamin D. Baldwin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The impetus for this research was the increasing threat of catastrophic wildfires resulting from the accumulation of fuels across the West. Guided by the priorities, goals, and guiding principles outlined by the national fire plan (NFP), the objective was to identify those areas within a pinyon-juniper woodland-dominated landscape with the highest hazard of catastrophic wildfire. The intent was to help managers prioritize proactive fuels management efforts outside of the wildland urban interface (WUI). Based on a management paradigm, constraints were placed on the data collection, analysis, and model development. A geographic information system (GIS) was used to create a hazard …

Rapid Effects Of 25-Hydroxyvitamin D3 On Calcium Uptake In Isolated Chick Enterocytes, Ruta Phadnis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

25-Hydroxyvitamin D₃[25(OH)D₃] is a metabolite of vitamin D₃ that has long been considered to be an inactive precursor of the hormonally active metabolite 1,25- dihydroxyvitamin D₃[l,25(OH)₂D₃]; consequently very few studies have addressed the potential biological activity of 25(OH)₂D₃. However, it is known that 100 nM 25(OH)₂D₃ increases calcium transport in the perfused duodenal loop of the chicken to 200% of controls within 20 minutes. The hypothesis of the current study is that 25(OH)₂D₃ may be a hormonally active metabolite …

The Influence Of Fat And Water On The Melted Cheese Characteristics Of Mozzarella Cheese, Robert Lloyd Fife

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of reducing the fat content of low-moisture part-skim Mozzarella cheese from 19% to less than 5% on melted cheese properties, i.e., apparent viscosity, cheese melt, and cook color, was investigated. Functional properties of melt and stretch and cook color were evaluated at d 1, 7, 14, and 28. A rapid microwave oven method underestimated the moisture content of the low fat cheeses by approximately 10%. Low fat cheese did not melt as well as did the low-moisture part-skim Mozzarella cheese although the moisture content of the low fat cheese (moisture content ranged from 62.5% to 63.6%) was greater …

Assessing The Effects Of Myxobolus Cerebralis And Other Environmental Factors On The Dynamics, Abundance, And Distribution Of Trout Populations In The Logan River, Utah, Ernesto A. De La Hoz Franco

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The presence of nonnative trout and the recent introduction of Myxobolus cerebralis in the Logan River drainage pose a threat to the native Bonneville cutthroat trout population (Oncorhynchus clarki Utah). The variability in the response of susceptible trout populations to M. cerebralis, causing agent of whirling disease, suggests that environmental factors may influence the effects of the parasite in infected environments. I investigated the relationship between temperature, discharge, substrate size, nutrient concentration (nitrogen and phosphorus), periphyton (chlorophyll a), and the relative abundance of Tubifex tubifex to the distribution, and prevalence of M. cerebralis in wild salmonid populations and …

Species Pairwise Associations Over Nine Years Of Secondary Succession: Assessing Alternative Explanations And Successional Mechanisms, Lara R. Rozzell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The importance and mechanisms of species interactions are undetermined in most successional systems. I used correlations and null modeling to detect pairwise species associations between 33 plant species in the first nine years of secondary succession after logging and burning in a western Oregon Cascade forest. I tested for correlations between each species and soil nutrients, nonvegetative ground cover, and surrounding vegetation. More positive than negative associations were found at all sampling times. The proportion of positive associations decreased and negative associations increased through time. Up to 42% of associations at a sampling time were explicable by shared positive correlations …

Physiological And Biochemical Aspects Of Agrobacterium-Wheat (Triticum Aestivum L.) Interactions, David L. Parrott Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Agrobacterium tumefaciens and A. rhizogenes are the causal agents of gall or hairy root disease, but normally the bacteria do not cause disease in wheat. However, both bacteria grew without inhibition when exposed to intact or wounded wheat roots or embryos, and they colonized wheat root surfaces to levels similar to dicotyledonous plants. A. tumefaciens and A. rhizogenes induced 23% cell death after a 1-h exposure to wheat embryo cells grown in 7.4 mM O₂, while the extent of cell death at 2.1 mM O₂ was 8%. Contact with A. tumefaciens or A. rhizogenes caused cultured wheat …

Foraging Behavior And Habitat Use Patterns Of Brown Bears (Ursus Arctos) In Relation To Human Activity And Salmon Abundance On A Coastal Alaskan Salmon Stream, Anthony P. Crupi Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Over the past decade, demand for recreation has increased as part of Alaska's doubling growth in tourism. Along the Chilkoot River, near Haines, fishing and bear viewing have become increasingly popular. I investigated the ecological and behavioral interactions there between brown bears, salmon, and humans between 2000 and 2002. My objectives were to: (1) determine if specific human activities differentially influenced bear activity and foraging behavior, (2) identify temporal and spatial habitat use patterns, (3) evaluate brown bear response to natural and human disturbances and quantify related flight distances, (4) investigate changes in bear foraging behaviors in response to prey …

An Ecological/Life History Comparison Of Two Whitefish Species In Bear Lake, Utah/Idaho, Brett W. Thompson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ecological traits of the endemic Bear Lake whitefish Prosoopium abyssicola and the Bonneville whitefish prosopium silonotus were investigated. Spatial distributions indicated distinctive differences in depth contour preference. Catch per unit effort data indicated that Bonneville whitefish prefer shallow depths and warmer water temperatures, whereas Bear Lake whitefish prefer deep, cold water.

Diet differences between the two species were large. Differences in both age distribution and growth rate patterns were also observed. The Bonneville whitefish population was predominantly composed of juvenile age classes. Very large adults reached ages of 12-14 years. Bear Lake whitefish exhibited a different population structure with few …

Factors Affecting Spawning And Survival Of Bear Lake Bonneville Cutthroat Trout In St. Charles Creek, Idaho, Paul Burnett

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I described the spawning ecology of the Bear Lake Bonneville cutthroat trout (BLBCT) in St. Charles Creek. I tracked cutthroat trout with used radio telemetry. I conducted redd counts to describe spawning conditions. Most cutthroat trout in the Big Arm strayed into the Bear River. Cutthroat trout migrations in the Little Arm and main fork were very limited (< 4 km). Redd distributions showed very similar patterns between 1989, 2000 and 2001 with most redds being built in the lowest kilometer of stream. Artificial fish transportation changed the redd distribution in 2002. More redds were built in the main fork and redds were distributed throughout the stream. Redds built in the main fork were characterized by lower levels of fine sediment and higher water velocities as compared to the redds built on the Little Arm. The results of this research will be used to aid resource managers in developing a management plan for wild BLBCT.

Effect Of Drought, Flooding, And Potassium Stress On The Quantity And Composition Of Root Exudates In Axenic Culture, Amelia Henry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Root exudates include important chelating compounds and can change the rhizosphere pH by several units. These changes are essential for nutrient uptake and can also alter solubility of soil contaminants and increase plant uptake. Mild root-zone stress may increase exudation and more severe stress can damage membranes and increase root turnover, all of which increase root-zone carbon. Increased carbon from this rhizodeposition can increase microbial activity, which might help degrade contaminants. We studied the effect of three types of stress on root exudation of crested wheatgrass (Agropyron cristatum): low K⁺, drought, and flooding. These stresses were …

Use Of Ground-Based Canopy Reflectance To Determine Radiation Capture, Nitrogen And Water Status, And Final Yield In Wheat, Glen L. Ritchie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ground-based spectral imaging devices offer an important supplement to satellite imagery. Hand-held, ground-based sensors allow rapid, inexpensive measurements that are not affected by the earth’s atmosphere. They also provide a basis for high altitude spectral indices.

We quantified the spectral reflectance characteristics of hard red spring wheat (Triticum aestivum cv. Westbred 936) in research plots subjected to either nitrogen or water stress in a two year study. Both types of stress reduced ground cover, which was evaluated by digital photography and compared with ten spectral reflectance indices. On plots with a similar soil background, simple indices such as the …

Correlation Between The Usu Stretch Test And The Pizza Fork Test, Bryce L. Moyes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A correlation between the USU stretch test and the pizza fork test would allow the stretch properties of Mozzarella cheese to be measured objectively. The Utah State University (USU) Stretch Test uses a modified texture-profile analyzer to pull strands of cheese from a melted reservoir, measuring the load exerted on the probe during stretching. Parameters measured by the USU stretch test include Melt Strength (F_M), which is the maximum load exerted on the probe, Stretch Load (SL), which is the load exerted on the probe at any specified point following F_M, and Stretch Extension (SE), which …

Rapid Detection Of Listeria Monocytogenes, Wim Lippens

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Listeria monocytogenes is a foodbome pathogen that can cause severe illness and even death. It is found in dairy and meat products. The focus is on rapid detection since conventional methods are time consuming (4-5 days). Pre-enrichment steps, as part of those methods, are time consuming. Our objective was to develop a detection system without a pre-enrichment step, giving a final result within 2 to 4 h.

In the concept of "the need for speed," a detection system with an antibody-based capture technique, followed by polymerase chain reaction (PCR), was developed. Glass beads coated with a Listeria polyclonal antibody were …

Production Of Volatile Sulfur Compounds From Inorganic Sulfur By Lactococci, Supriyo Ghosh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Production of volatile sulfur compounds in cheese is associated with desirable flavors. The direct source of these compounds has been assumed to arise from the metabolism of methionine and cysteine. However, the methionine concentration in cheese rises above the amount found in casein during aging, suggesting that alternative sulfur sources are present in milk. This led us to hypothesize that lactococci may acquire sulfur from the inorganic sulfur pool of milk, in addition to methionine and cysteine, to generate volatile sulfur compounds during cheese ripening.

A turbidimetric method to determine total sulfate content in milk samples was developed. The average …

The Effects Of Alternative-Site Blood Glucose Monitoring On Testing Frequency, Pain Rating, And Glycosylated Hemoglobin, Nancy Bennion

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A crossover design study was conducted to determine if reducing pain, by using alternative sites off the finger tip, would increase testing frequency and improve clinical outcome as measured by glycosylated hemoglobin. Subjects with type 1 and type 2 diabetes tested with the FreeStyle alternative-site meter (group 1) or tested with their original meter (group 2). After 3 months the subjects used the alternate meter. Testing frequency and blood glucose concentrations were recorded for the month before the study began and monthly thereafter. Glycosylated hemoglobin was tested initially, at the crossover point, and at study conclusion. Insulin users increased testing …