Life Sciences Commons^™

Causes And Consequences Of Plant Spatial Patterns On Natural And Experimental Great Basin (Usa) Plant Communities, Andrew P. Rayburn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The processes by which plant spatial patterns are formed, and the effects of those patterns on plant community dynamics, remain important areas of research in plant ecology. Plant spatial pattern formation has been linked to many ecological processes that act to structure plant communities at different spatiotemporal scales. Past studies of pattern formation are common, but recent methodological advances in data collection and analysis have permitted researchers to conduct more advanced observational studies of pattern formation in space and time. While studies of the effects of plant spatial patterns were formally rare, they have increased in the last decade as …

Reducing Reliance On Supplemental Winter Feeding In Elk (Cervus Canadensis): An Applied Management Experiment At Deseret Land And Livestock Ranch, Utah, Dax L. Mangus

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildlife managers have fed elk in North America for nearly 100 years. Giving winter feed to elk can compensate for a shortage of natural winter range and may boost elk populations while also helping prevent commingling with livestock and depredation of winter feed intended for livestock. In contrast to these benefits of supplemental feeding, there are economic and environmental costs associated with feeding, and elk herds that winter on feeding grounds have a higher risk of contracting and transmitting disease. Brucellosis is of primary concern now, and Chronic Wasting Disease may be in the future. Many see the discontinuation of …

Effects Of Non‐Surface‐Disturbing Treatments For Native Grass Revegetation On Cheatgrass (Bromus Tectorum L.) Metrics And Soil Ion Availabilities, Jan C.R. Summerhays

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several restoration methods intended to increase the success of aerially‐seeded perennial grasses were assessed to determine their effects on cheatgrass metrics and soil nutrient bioavailabilities. These methods were: 1) imazapic herbicide application (140 g ai ∙ ha^-1, 210 g ai ∙ ha^-1, and no application [control]), 2) vegetation manipulation treatments (50% sagebrush overstory thinning, 100% sagebrush overstory thinning, sagebrush overstory and/or vegetative thatch burning, and no manipulation [control]), and 3) alternative seeding treatments (aerial seeding with raking, aerial seeding with activated carbon [AC] addition, aerial seeding with sucrose addition, and regular aerial seeding [control]). Treatments were …

Characterization Of Volatile And Metabolite Compounds Produced By Lactococcus Lactis In Low-Fat And Full-Fat Cheddar Cheese Extract, Michael J. Young

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to compare and contrast potential aroma compounds in the headspace and small molecule metabolites produced as a result of starter culture metabolism in a full-fat and low-fat cheddar cheese model system. Past studies have indicated differences in the headspace flavor compound profiles between full-fat and low-fat Cheddar cheeses with no indication as to what compounds were produced as a result of starter culture metabolism.

Starter cultures were incubated in a Cheddar cheese extract environment that was made up of the water-soluble portion of Cheddar cheese with environmental conditions mimicking full-fat and low-fat Cheddar cheese by altering …

Effect Of Adjunct Cultures, Sodium Gluconate, And Ripening Temperature On Low-Fat Cheddar Cheese Flavor, Rebekah M. Lance

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Low-fat Cheddar cheese flavor is different from full-fat Cheddar cheese and is not acceptable to many consumers. This 2-part experiment was designed to examine effects adjunct cultures have on low-fat Cheddar cheese flavor as determined through descriptive analysis and consumer feedback.

In Part 1, low-fat (5%) Cheddar cheese was produced in duplicate, using 6 combinations of DVS850, LH32, CR540, CRL431, Emfour, and CR319 bacterial cultures. Due to a previously observed positive effect by sodium gluconate on low-fat cheese flavor, each replicate was split into treatments of 0.0% and 0.8% sodium gluconate. Each of these treatments was then split into ripening …

Evaluation Of Levulinic Acid For Topical Decontamination Of Meat Surfaces, Jeffrey V. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experiments were performed to investigate the effects of wash treatments, consisting of hot water, 2% lactic, 2% acetic, or 2% levulinic acid, for decontamination of pathogenic bacteria previously inoculated onto meat surfaces, to inhibit growth of pathogenic bacteria inoculated onto previously washed meat surfaces, and on the organoleptic quality of sliced turkey roll and beef trim. Acid washes were no more effective at reducing Escherichia coli O157:H7 on beef plate, Listeria monocytogenes on sliced turkey roll, and Salmonella on pork belly than was water wash. Only lactic acid treatment was more effective than water at reducing Salmonella on chicken skin, …

Studies Of The Distinguishing Features Of Nadph:2-Ketopropyl-Coenzyme M Oxidoreductase/Carboxylase, An Atypical Member Of The Disulfide/ Oxidoreductase Family Of Enzymes, Melissa A. Beighley-Kofoed

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The metabolism of propylene in Xanthobacter autotrophicus occurs via epoxypropane formation and subsequent metabolism by a three-step, four-enzyme pathway, utilizing the atypical cofactor Coenzyme M (CoM) to form acetoacetate. The last step in the epoxide carboxylase pathway is catalyzed by a distinctive member of the disulfide oxidoreductase (DSOR) family of enzymes, NADPH:2-ketopropyl CoM oxidoreductase/carboxylase (2-KPCC). 2-KPCC catalyzes the unorthodox cleavage of a thioether bond and successive carboxylation of the substrate. The focus of the research presented in this dissertation aims to elucidate the details of 2-KPCC that allow it to perform chemistry unconventional for typical DSOR members. Sitedirected mutagenesis was …

Community Level Effects Of Vegetation Architecture And Prey Availability: A Study Of Ground-Dwelling Arthropods In A Shrub-Steppe Ecosystem, Mary E. Pendergast

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes to vegetation architecture within a natural habitat can have profound impacts upon ecological community function, but the relative influence of vegetation architecture itself and potential indirect influences of associated food resources are often difficult to disentangle. I present the results of a three-year study designed to address the community level impacts of changes in big sagebrush (Artemisia tridentata) architecture and associated prey availability upon the ground-dwelling arthropod community. Three experimental sagebrush architecture treatments (low, intermediate, and high foliage density) and two levels of prey insect availability treatment (natural and baited/increased prey availability) were imposed in a sage-steppe …

The Decline And Conservation Status Of North American Bumble Bees, Jonathan B. Koch

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several reports of North American bumble bee (Bombus Latreille) decline have been documented across the continent, but no study has fully assessed the geographic scope of decline. In this study I discuss the importance of Natural History Collections (NHC) in estimating historic bumble bee distributions and abundances, as well as in informing current surveys. To estimate changes in distribution and relative abundance I compare historic data assembled from a >73,000 specimen database with a contemporary 3-year survey of North American bumble bees across 382 locations in the contiguous U.S.A. Based on my results, four historically abundant bumble bees, B. …

A Sensory-Based Multi-Component School-Based Nutrition Intervention Among Fifth-Grade Students, Stacy Lyn Bevan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The nutritional status of children is declining as evidenced by the steady rise in childhood obesity rates over the last three decades. Consuming five servings of fruit and vegetables (FV) daily has shown to help with weight maintenance, but children are consuming far less than the recommended servings. This study was designed to test the efficacy of a sensory-focused multi-component school-based program at increasing vegetable intake among fifth-graders. Classroom, family, and community components allowed children to explore thirteen target vegetables with their senses including taste. Vegetable consumption was measured by digitalized observations of lunchtime vegetable selection and consumption. Vegetable acceptance …

Ungulate Damage To Safflower In San Juan County, Utah, Michael J. Haney

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In Utah, farmers are concerned that ungulates are damaging safflower (Carthamus tinctorius) fields. I examined elk (Cervus elaphus) and mule deer (Odocoileus hemionus) damage to safflower production in San Juan County, Utah during 2009 and 2010. Data on damaged safflower plants were collected within 28 fields, totaling 1,581 ha (13 fields totaling 963 ha during 2009; 15 fields totaling 618 ha during 2010). I compared 3 methods to assess losses: ungulate-proof exclosures, adjacent plant compensation method, and counting the number of damaged plants in 50-m transects (safflower count method). Exclosures were of limited use …

Invertebrate Community Changes Along Coqui Invasion Fronts In Hawaii, Ryan T. Choi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Puerto Rican coqui frog, Eleutherodactylus coqui, was introduced to Hawaii in the late 1980s via the commercial horticulture trade. Previous research has shown that coquis can change invertebrate communities, but these studies were conducted at small scales using controlled, manipulative experiments. The objective of this research was to determine whether coqui invasions change invertebrate communities at the landscape scale across the island of Hawaii. At each invasion front, we measured environmental variability on either side of the front and removed sites that were too variable across the front to ensure that the impacts we measured were the result …

The Role Of Phosphoinositide Signaling In Breast Cancer Metastasis Suppressor 1-Mediated Metastasis Suppression Of Human Breast Carcinoma Cells, Sitaram Harihar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Breast cancer is the most common non-skin cancer in women and the second most common cause of cancer-related death in U.S. women. Despite numerous advances in treatment strategies against breast cancer, the presence of undetected distant metastasis of the primary tumor remains the main cause of mortality. Current screening and detection methods such as mammograms are simply not sensitive enough to detect formation of metastasis. Further, currently available therapies against metastatic breast cancer do not provide a complete cure for the disease. Thus, understanding the biology and molecular factors involved in cancer metastasis will help aid in preventing the onset …

The Transfer Of Agricultural Water To Municipal And Industrial Usages, Dallin Paul Stephens

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The water that is available for beneficial use in Utah is quickly approaching full appropriation; water that has been claimed is nearing the amount that is available for use. The Division of Water Resources of the State of Utah has organized a three-part plan to "Plan, Conserve, Develop and Protect Utah's Water Resources." One of these three elements has a focus to "provide comprehensive water planning." Such planning is best achieved when current and accurate data on the uses of the state's water are available.

The primary purpose of this thesis was to provide an evaluation, from data collected on …

Development Of A Beef Flavor Lexicon And Its Application To Compare Flavor Profiles And Consumer Acceptance Of Grain- And Pasture-Finished Cattle, Curtis A.J. Maughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Flavor lexicons are used in sensory evaluation to determine the flavor profile of a food product. The objective of this study was to develop a flavor lexicon for cooked beef, which can then be used in various projects relating to beef quality such as studies investigating animal diet, marinating, ageing, or other enhancements. A descriptive panel of 10 people was used to develop a flavor lexicon of 18 attributes, including astringent, barny, bloody, brothy, browned, gamey, grassy, juicy, fatty, livery, metallic, oxidized, roast beef, and the five basic tastes (bitter, salty, sour, sweet, and umami). In contrast to other studies …

The Effectiveness Of A Structured, Long-Term, Multi-Component, Family-Based Weight Management Program In Reducing Body Mass Index Z-Scores And Improving Lifestyle Habits In Overweight/Obese Children And Adolescents, D. Pauline Williams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Few studies have evaluated structured, long-term, family-based, weight management programs in children. This study’s purpose was to determine if completion of such a program resulted in reduced body mass index (BMI) z-scores and improved lifestyle habits.

An observational study overtime from 2008-2010 was conducted with 89 overweight/obese children. Subjects were divided into two groups, compliers (completed ≥ 7 intervention classes) and non-compliers (completed < 7 intervention classes.) The LiVe program, a 12-month structured, multi-component, family-based program, served as the study intervention. Anthropometric measurements and a written survey on lifestyle habits were used to obtain changes over 12 months in anthropometric, nutrition, activity, and behavior habits for subjects.

Descriptive statistics, chi square, analysis of co-variance, and a mixed model logistic regression were used to determine anthropometric and lifestyle habits change over time as well as differences between age, gender, and compliance groups. No demographic differences …

Individualistic Response Of Piñon And Juniper Tree Species Distributions To Climate Change In North America's Arid Interior West, Jacob R. Gibson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Piñon and juniper tree species have species-specific climatic requirements, resulting in unique distributions and differential responses to climate change. Piñons and junipers co-dominate the arid woodlands of North America as groups with widespread hybridization. Two piñons, Pinus edulis; P. monophylla, and four junipers, Juniperus deppeana var. deppeana; J. monosperma; J. occidentalis; J. osteosperma, are endemic to the midlatitude interior west and form three groups of hybridizing sister species, P. edulis-P. monophylla; J. deppeana var. deppeana-J. monosperma; J. occidentalis-J. osteosperma. Recent droughts have caused widespread mortality among piñons, but have had less impact on …

Riparian Bird-Habitat Association Models: A Framework For Informing Management And Developing Restoration Guidelines In Utah, Hillary M. White

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Approximately 75% of the avian species in Utah use riparian habitats at some time during their life cycles and at least 80% of this habitat in Utah has been lost or altered since settlement; currently 0.6% of land cover in Utah is considered riparian. In 1992, with the support of Utah Partner's in Flight, the Utah Division of Wildlife Resources began a statewide neotropical migratory bird (NTMB) and habitat monitoring program to assess the status of bird populations at 31 sites. Additional sites (up to 52) were added in later years; bird and habitat assessments at 37 riparian sites have …

Behavioral Responses Of Willow Flycatchers, Empidonax Traillii, To A Heterogeneous Environment, Amanda V. Bakian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spatial heterogeneity impacts population and community-level dynamics including species-level dispersal patterns, the use and availability of refugia, predator/prey dynamics, and reproductive fitness. Understanding how wild animal populations respond to environmental heterogeneity is essential for their proper management and conservation. In this study, I examine the responses of Willow Flycatchers to spatial heterogeneity in the distribution of their food and habitat resources. Over the course of three breeding seasons, I radio- tracked Willow Flycatchers at Fish Creek in Manti-La Sal National Forest in Utah, recorded detailed behavior data at each radio location, and collected fecal, feather and insect samples. I formulated …

Habitat Characteristics And Occupancy Rates Of Lewis's Woodpecker In Aspen, Amy M. Vande Voort

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lewis‘ woodpeckers (Melanerpes lewis) are generally associated with open ponderosa pine (Pinus ponderosa), open riparian, and burned pine habitats in the West; however, this species has recently been found to nest in aspen (Populus tremuloides) stands in Utah. This study describes the habitat characteristics of Lewis‘ woodpecker nest sites in aspen and investigates how well aspen stand characteristics predict Lewis‘ woodpecker occupancy. I surveyed for Lewis‘ woodpeckers at previously occupied nesting locations in aspen and took habitat measurements at nest sites. In addition, nest-centered Forest Inventory and Analysis (FIA)-type plots provided stand-level habitat characteristics. I used logistic regression to …

Characterization And Application Of Dynamic In Vitro Models Of Human Airway, Hemangkumar J. Patel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In recent years, respiratory diseases have emerged as a leading cause of mortality across the globe. In the United States alone respiratory diseases are the fourth leading cause of deaths annually. Moreover, with the rapid increase of industrialization and urbanization, the occurrences of respiratory diseases are expected to remain high with strong chances of increasing in the future. To ameliorate the epidemic of respiratory disease, it is first important to understand its underlying mechanisms.

Respiratory research studies in animals have elucidated the chronological order of the pathological events and systemic responses inside the lung, but understanding the response of individual …

Spatiotemporal Modeling Of Threats To Big Sagebrush Ecological Sites In Northern Utah, Alexander J. Hernandez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study tested the performance of classification, regression, and ordination techniques to evaluate the spatiotemporal dynamics of threats to big sagebrush ecological sites. The research was focused on invasion by annual exotic grasses and encroachment by woodlands.

We sought to identify those areas that have had a persistent coverage of cheatgrass (Bromus tectorum) in big sagebrush ecological sites. We took advantage of the contrast in greenness between multi-temporal (within one year) remotely sensed vegetation indices captured in the spring and summer to find a distinct phenological signature that allowed mapping cheatgrass. We utilized support vector machines (SVM) to classify three …

An Evaluation Of State-And-Transition Model Development For Ecological Sites In Northern Utah, Jamin K. Johanson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ecological sites and state-transition models (STMs) have become the preferred means of summarizing plant community dynamics on distinctive types of rangeland. Ecological sites classify rangeland types based on soil-geomorphic and climatic conditions capable of producing a known plant community, while a STM depicts the vegetation dynamics of an ecological site. STMs are usually based on expert opinion rather than site-specific data; however, if they are to gain credibility, STMs must accurately describe the processes that drive plant community dynamics. This study examined three ways of developing process-based STMs using three levels of commonly collected field data. We began by taking …

Microbiological, Thermal Inactivation, And Sensory Characteristics Of Beef Eye-Of-Round Subprimals And Steaks Processed With High-Pressure Needleless Injection, Laura Kahealani Jefferies

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

High-pressure needleless injection (HPNI) is a process where small-diameter, high-velocity burst of liquid, penetrate foods at pressures ≤ 10,000 psi. The potential of HPNI as an enhancing technique for meat was studied. In study 1, HPNI translocated surface E. coli O157 into the interior of beef eye-of-round subprimals with an incidence of 40 (±7), 25 (±8), and 25 (±8)% for meat that had been surface-inoculated with a four-strain cocktail at 0.5, 1, and 2 log₁₀ CFU/cm², respectively. Run-off water contained 2, 2, and 3 log₁₀ CFU/ml and was used for HPNI of additional subprimals, which resulted …

Temperature Increase Effects On Sagebrush Ecosystem Forbs: Experimental Evidence And Range Manager Perspectives, Hilary Louise Whitcomb

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sagebrush plant communities are among the most threatened in North America. This project had two goals: to test how increased temperature affects native and nonnative forb species common to the Western sagebrush region and to evaluate land manager beliefs about changes in their ecosystems, including those affecting forb species.

Native forbs Sphaeralcea munroana, Crepis acuminata, Linum lewisii, Penstemon palmeri, and Oenothera pallida and non-natives Erodium cicutarium and Lactuca serriola were each subjected to two treatments: experimental warming using open-top chambers and a control. Knowing how forbs used in restoration might respond to future conditions is both …

Diet, Density, And Distribution Of The Introduced Greenhouse Frog, Eleutherodactylus Planirostris, On The Island Of Hawaii, Christina A. Olson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The greenhouse frog, Eleutherodactylus planirostris, native to Cuba and the Bahamas, was recently introduced to Hawaii. Studies from other invaded habitats suggest that it may impact Hawaiian ecosystems by consuming and potentially reducing endemic invertebrates. However, there have been no studies on the greenhouse frog in Hawaii. The first component of this study was to conduct a diet analysis. We conducted a stomach content analysis of 427 frogs from 10 study sites on the island of Hawaii. At each site, we also collected invertebrates using two different sampling methods: leaf litter collection and sticky traps to characterize available resources. …

Design Of A BiobrickTm Compatible Gene Expression System For Rhodobacter Sphaeroides, Junling Huo

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The concept of introducing engineering principles of abstraction and standardization into synthetic biology has received increasing attention in the past several years and continues to be in the forefront of synthetic biology. One direction being pursued by synthetic biologists is creation of modular biological parts (BioBrick^TM) that can be readily synthesized and mixed together in different combinations. However, most standard BioBrick^TM parts in the Registry were designed for E. coli, although synthesis of specific BioBrick^TM parts for other bacteria, such as for yeast and cyanobacteria, have begun. Besides, at the present time, there are only …

Stress Response And Pathogenesis Of Salmonella Enterica Serovar Typhimurium, Jigna D. Shah

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salmonella is a food-borne pathogen that leads to substantial illness worldwide. The clinical syndromes associated with Salmonella infection are enteric (typhoid) fever and gastroenteritis, in healthy humans. Typhoid fever is caused by host-adapted S. Typhi and S. Paratyphi. Gastroenteritis is caused by serovars usually referred to as non typhoidal Salmonellae (NTS). In recent years, an increasing number of outbreaks due to NTS, despite increased efforts in food safety, were reported because of persistence of Salmonella in the food chain. Thus I hypothesized that Salmonella is able to withstand stresses in the environment and treatments used during food processing …

A Study On The Applications And Toxicity Assessments Of Carbon Nanotubes In Tissue Engineering, Rena Baktur

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Carbon nanotubes (CNTs) are one of the most popular nanomaterials. There has been increasing interest in the development and applications of carbon nanotubes due to their huge potential in industrial and medical applications. Recent applications of carbon nanotubes include development of scaffolds and drug delivery systems. Despite rapidly emerging applications of CNTs, little is known about the impact of CNTs on cellular processes, especially mesenchymal stem cell (MSC)'s differentiation. Also, the effects of nanoparticle exposure under different conditions on cellular responses have not been well characterized yet.

To characterize the effects of CNTs on creating nanoscale scaffolds for tissue engineering, …

15n Tracer And Modeling Analyses Of Nutrient Transport Through Lakes In A Subalpine Watershed, David M. Epstein

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lakes have historically been overlooked as important nutrient processors within their watersheds. In general ecologists have focused on streams as zones of uptake and transformation, while viewing lakes as simple nutrient traps. However, recent research has highlighted the large influence that lakes may have on water chemistry within their watersheds. Within the field of limnology, researchers have traditionally focused on the pelagic zone for in-lake production. Further research in shallow lakes has highlighted the role benthic production within the littoral zone plays in the lake ecosystem. The greater influence of lakes is highlighted when comparing watersheds containing lakes with watersheds …