Life Sciences Commons^™

Kisspeptins - Expression, Purification, And Biological Assays, Mathew Puthayathu Kurian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Kisspeptins are hormones involved in the onset of puberty in adolescents and maintenance of reproductive function in adults. Defects in kisspeptin signaling are associated with many reproductive disorders. Kisspeptins were originally discovered as metastasis suppressors and have been found to suppress the metastasis of certain cancers. Unfortunately, many aspects of kisspeptin action at a cellular level remain unknown. Kisspeptins, therefore, are attractive candidates for large-scale production for use as research and therapeutic reagents. We developed a simple and cost effective method to express and purify kisspeptins in large quantities. We also tested the biological activity of kisspeptins on cells expressing …

Characterization Of The Product Specificity And Kinetic Mechanism Of Protein Arginine Methyltransferase 1, Shanying Gui

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protein enzymes perform a vast array of functions within living organisms, catalyzing various metabolic reactions including DNA replication, DNA repair, protein synthesis, etc. In order to maintain proper cellular functions, enzymes need to be accurately regulated under different circumstances. Specifically, enzymes can be modified after their creation to give them additional functions. These modifications can do a variety of things including activating (turning on) or inactivating (turning off) an enzyme, changing what proteins or molecules can interact with the enzyme, changing the enzyme’s location in the cell, and/or targeting the enzyme for destruction. This dissertation focuses on a single class …

Assessing Vehicle-Related Mortality Of Mule Deer In Utah, Daniel D. Olson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Roads are essential in modern societies, but as populations grow and traffic volumes rise, roads will continue to be built and expanded. As a result, the effects that roads have on wildlife will likely intensify, making it imperative that managers understand those effects so mitigation can be directed accordingly. In Utah, considerable areas of mule deer (Odocoileus hemionus) habitat have been bisected by roads. Mule deer are commonly involved in vehicle collisions and there is concern that roads and vehicle traffic are impacting populations. This project was conducted to determine the number and demographic effects of deer-vehicle collisions, …

Evaluation Of Survey Methods And Development Of Species Distribution Models For Kit Foxes In The Great Basin Desert, Stephen J. Dempsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Historically, kit foxes (Vulpes macrotis) once occupied the desert and semi-arid regions of southwestern North America, ranging from Idaho to central Mexico. Their range-wide decline has warranted the kit fox to be designated as a state sensitive species in Utah. Once considered the most abundant carnivore in western Utah, the kit fox has been in steep decline over the past decade, creating a demand to determine kit fox presence in the Great Basin desert. Currently there is little consensus on which survey methodology is best for detecting kit fox presence. We tested 4 survey methods (scat deposition, scent …

Causes And Consequences Of Local Variability In Aroga Websteri Clarke Abundance Over Space And Time, Virginia L.J. Bolshakova

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sagebrush steppe habitat is rapidly declining in the Intermountain West in both quality and quantity. Observed reductions in animal and bird populations associated with sagebrush, invasion of exotic annual grasses such as cheatgrass (Bromus tectorum), and other concerns with climate change have led to a growing awareness of the need to monitor and better understand the rate of habitat loss. Sagebrush lands are subject to periodic loss caused by insect feeding damage, and human activities have the potential to promote unnaturally high levels of such loss. The sagebrush defoliating Aroga moth, Aroga websteri Clarke (Lepidoptera: Gelechiidae), can kill …

Vital Rates, Population Trends, And Habitat-Use Patterns Of A Translocated Greater Sage-Grouse Population: Implications For Future Translocations, Orrin V. Duvuvuei

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus; sage-grouse) populations have declined range-wide with some local populations exhibiting dramatic decreases. In 2009–2010, radio-marked hens (30 hens in 2009 and 30 hens in 2010) were translocated from Parker Mountain in south-central Utah to augment a rapidly declining population on Anthro Mountain in northeastern Utah. Thirty-two resident female sage-grouse on Anthro Mountain were also radio-collared from 2009–2012.

I compared population vital rates (i.e., nest and brood success, and survival) of resident and translocated hens in Anthro Mountain’s population to those of a translocated population in Strawberry Valley, Utah and the source population. Of these …

Integrated Management Of Downy Brome (Bromus Tectorum L.) Infested Rangeland, Heather Elwood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Invasive weed species are a threat to the health and functionality of many rangeland systems. Downy brome (Bromus tectorum) is an invasive annual grass that affects the productivity of rangelands by decreasing the grazing capacity for livestock as well as altering the wildfire cycle and competing against more desirable vegetation for limited resources.

In 2006, an Invasive Plant Management Plan and Environmental Assessment was approved for Dinosaur National Monument, calling for prioritization of invasive species management on high value wildlife habitat, vector areas, and for species with a high ecological impact. The Cub Creek Watershed was identified as a priority …

Ecology Of Coyotes On The Valles Caldera National Preserve, New Mexico: Implications For Elk Calf Recruitment, Suzanne J. Gifford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Valles Caldera National Preserve (VCNP) managers were concerned about low elk recruitment observed at the same time as an apparent increase in sightings of coyotes and observations of coyote predation on elk calves. The goal of this study was to describe coyotes’ ecological interactions with elk, particularly coyote diet and movements on the Valle Grande, a large grassland meadow in the southeastern portion of the VCNP.

We examined coyote diet by quantifying undigested remains of food items in coyote scats (feces). The most frequent taxa were rodents (montane voles and pocket gophers), elk (adult and calf), insects (grasshoppers and beetles), …

Peach Fruit Quality Analysis In Relation To Organic And Conventional Cultivation Techniques, Varun Chandra Koneru

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The USA is the third major world producer of peaches but consumption has decreased over the last two decades. Consumers have cited mealy texture, fruit browning and lack of sweetness as some undesirable characteristics in peaches, which may be related to the decline. The focus of this study was to evaluate the effect of farm management practices on fruit quality. Physical parameters (color, firmness and size), volatiles and metabolite data was collected.
Sensory evaluation indicated transitional organic peaches were liked the best and organically grown peaches were least liked. All the treatments were significantly different from each other and consumers …

The Effect Of Daily Released-Time Religious Education On Academic Achievement, Trace W. Hansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Various methods, programs, and efforts to educate students in effective and efficient ways have been employed and studied for many years in the United States. Many teachers, administrators, and communities seek to gain a better understanding of and implement programs that will help achieve the academic goals of their respective organizations. Previous research indicates a correlation between some types of classes, programs, and characteristics of students and their academic achievement. Data indicating academic information for three hundred and fifty suburban secondary school students were collected and analyzed to support or refute previous research in this area of study. Grade point …

Legacy Effects Of Habitat Degradation By Lesser Snow Geese On Ground-Nesting Savannah Sparrows Along The Hudson Bay Lowlands, Stephen L. Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Little is known of the direct and indirect legacy of Lesser Snow Goose (LSGO) habitat degradation in northern Canada on the biodiversity of other avian species. It is believed that a human-induced trophic cascade caused in part by agricultural habitat modification along migratory routes and wintering grounds has contributed to the increase in LSGO numbers, which has resulted in increased foraging pressure by LSGO on northern breeding and stopover sites. This habitat degradation may lead to decreased abundance and richness of Arctic / sub-Arctic avian species across landscapes that LSGO utilize and degrade.

Here we evaluated the annual change in …

Mule Deer And Wildlife Crossings In Utah, Usa, Megan Schwender

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildlife-vehicle collisions (WVCs) negatively impact wildlife populations and create dangerous driving situations for motorists. In Utah, USA, mule deer (Odocoileus hemionus) encounter a variety of hazards as they attempt to cross highways and interstates, some of which are 8 lanes wide. Agencies have sought to mitigate the risks posed to drivers and mule deer by building crossing structures for wildlife. The objectives of this study were to evaluate the effectiveness of crossing structures in Utah to safely pass mule deer under highways and to determine the variables that best explain mule deer passage use. From 2008 - 2011 …

Nutrition Education To Minimize Health Risk: Approaches For Teaching College Students And Female High School Athletes, Katie Nicole Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Obesity, heart disease, and osteoporosis are among the major health concerns facing Americans today. Obesity, elevated blood cholesterol, and even atherosclerosis are affecting America’s children and adolescents and putting them at increased risk for heart disease later in life. Similarly, low bone mineral density is seen among some adolescents including female athletes, increases their risk for immediate and lifetime fracture. Female athletes who do not consume enough calories to compensate for calories expended during physical activity are in a state of low energy availability which can lead to menstrual dysfunction, as well as bone loss, and injury (the female athlete …

Factors That Influence Secondary Students To Join The Collegiate Ffa, Sara Vanderbos

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Student involvement on college campuses is important for the professional growth, leadership development and learning of today's college student. This research sought to determine the factors that influence former high school FFA members' reasons for joining and participating in the Collegiate FFA.

The study showed that students who are involved in the National FFA at the secondary level are more likely to join Collegiate FFA while attending a university that offers the Collegiate FFA option. These students were actively engaged on campus and were interested in helping others. Collegiate FFA programs, the National FFA, and universities across the country should …

Physiological Response Of Kentucky Bluegrass Under Salinity Stress, Lijun Wang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salinity is a significant stress for plants world-wide. In agriculture, salts reduce germination, overall growth, yield, and sometimes death in crop plants. Salinity similarly affects turfgrass in our urban landscapes. Kentucky bluegrass (Poa pratensis L.) is the most widely used cool-season grass in the northern part of the United States, including the cool-arid West, but generally is a salt sensitive species. The overall objectives of this study were to study the physiological responses of Kentucky bluegrass to salt stress and to evaluate the genetic similarity among the cultivars used in the research.

Four Kentucky bluegrass entries, two salt-tolerant and …

Reproductive Biology And Impacts Of Energy Development On Physaria Congesta And Physaria Obcordata (Brassicaceae), Two Rare And Threatened Plants In The Piceance Basin, Colorado, Sarah Lynn Clark

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Oil and gas development has increased profoundly over the last 20 years in the United States. A large underground deposit of natural gas has been found in the Piceance Basin, which is located in the northwestern part of the state of Colorado. This deposit occurs in an area inhabited by two rare mustard species commonly named the Dudley Bluffs bladderpod and the Dudley Bluffs twinpod. These two plant species are also listed as threatened by the US Fish and Wildlife Service. Concerns about gas development effects on these rare plants have motivated research that quantifies these potential impacts. Through funding …

Local And Regional Drivers Of Biodiversity: From Life-History Traits To System-Level Properties, Sarah R. Supp

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Biodiversity research includes the study of where species occur, the commonness and rarity of species, the number of species, and the diversity of life-history traits that occur in a single location, or community. Research is increasingly recognizing that a combination of local and regional scale processes influence community dynamics over ecological and evolutionary time-scales. However, ecologists currently lack a comprehensive understanding of the mechanisms driving biodiversity in different systems and at different spatial scales. This presents a critical problem because without understanding the important mechanisms that determine and maintain biodiversity, it is difficult to accurately predict community response to environmental …

Evaluation Of The Tgf-Ss Inhibitor Repsox On The Expression Of Pluripotency Pathways In Murine And Bovine Cells, Davin M. Larsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Embryonic stem cells are cells which are isolated from early stage embryos and have the theoretical ability to become any adult cell type in the body. In the past few years much research has focused on the use of embryonic stem cells for clinical applications in the treatment of degenerative diseases. Consequently they are a promising tool in the treatment of diseases such as Diabetes and Parkinson’s disease, and could potentially be used in the repair of permanently damaged tissue. However, since these cells are isolated from early stage embryos their successful isolation often results in the destruction of the …

Forest Recovery, Nutrient Cycling And Carbon Sequestration In A Southern Appalachian Spruce-Fir Forest, Patrick T. Moore

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our forests provide us with a variety of services from clean water, forest products and wildlife habitat to the lesser known functions of nutrient cycling and carbon
sequestration. This research helps to demonstrate the extent of some of these services in a heavily disturbed southern Appalachian spruce-fir forest within Great Smoky Mountain National Park, the most heavily visited National Park in the United States. Following a catastrophic infestation of the non-native balsam wooly adelgid, the future of this forest was unknown, causing some to speculate about the future of this sensitive forest type. Though predictions about this forest’s future varied …

Comparison Of Topographic Surveying Techniques In Streams, Sara G. Bangen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Bonneville Power Administration (BPA) Fish and Wildlife Program (FWP) mitigates impacts, including mortality, from hydroelectric dams in the Columbia River Basin for ESA-listed salmon and steelhead populations and other species of special concern. Given the extensive economic resources invested in mitigation and the incredible diversity of in-stream habitat across the Columbia River basin, questions have arisen about which sampling strategies are most tractable across the entire Columbia River Watershed, but also produce datasets that allow researchers to answer meaningful questions about salmonid populations and trends in habitat. In response to these issues, the NOAA Integrated Status and Effectiveness Monitoring …

A Multi-Scale Investigation Of Factors Limiting Bull Trout Viability, Tracy Bowerman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Recent declines in many species of aquatic organisms have raised concerns about loss of biodiversity in river systems and the need to protect populations in peril. To conserve endangered species, scientists need to know information about the habitats organisms use throughout their life cycle and how environmental stressors cause populations to grow or decline. The goal of this research was to improve our understanding of the life-cycle requirements for bull trout (Salvelinus confluentus), a threatened freshwater fish species. I assessed environmental factors that affect bull trout egg incubation success and quantified juvenile bull trout movement patterns and survival …

Increased Production And Extraction Efficiency Of Triacylglycerides From Microorganisms And An Enhanced Understanding Of The Pathways Involved In The Production Of Triacylglycerides And Fatty Alcohols, Robert M. Willis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The continued increase in the demand for fossil fuels combined with their ever dwindling supply has prompted the search for a suitable alternative fuel. The research contained within this dissertation seeks to increase the lipid (fat) content of cellular feedstocks, improve extraction efficiencies of lipids, and to understand the pathways involved in the production of fatty alcohols and triacylglycerides, compounds commonly used in many industrial processes, from microbial feedstocks. This work has been done in an attempt to increase the overall economic viability of microbial biofuels production.

The production of biofuels from microalgae used as a feedstock allows for the …

Effects Of Non-Surface-Disturbing Restoration Treatments On Native Grass Revegetation And Soil Seed Bank Composition In Cheatgrass-Invaded Sagebrush-Steppe Ecosystems, Alexandra D. Reinwald

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The sagebrush-steppe communities of the Great Basin have been dramatically transformed by the invasion of the non-native annual grass cheatgrass. In many areas of the Great Basin, this invasion has resulted in the loss of native plant species and ultimately the conversion to cheatgrass-dominated communities. As healthy sagebrush communities provide multiple ecosystem services such as diverse wildlife habitat, forage for cattle grazing, and water filtration, restoration of these communities is a high priority to landowners and land management agencies. Established perennial grasses can successfully compete with non-native annual grasses and increase the resistance of plant communities to invasion by non-native …

Characterization And Potential Utility Of Porcine Trophoblast-Derived Stem-Like Cells, Edison A. Suasnavas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In mammalian pregnancy, the placenta is a very important organ which helps to establish a healthy pregnancy. Its functions could be described in four points: 1) It allows the fetus to receive gases and nutrients from the mother in a safe way; 2) it lets the fetus get rid of waste through the mother's kidneys; 3) it releases essential pregnancy-related hormones and growth factors that let the uterus hold the fetus; 4) it secretes immune response regulators to give the fetus immune protection against the mother. Abnormalities in the placenta can be the cause of death to the fetus. Mammalian …

Needs Assessment For Promoting Livestock And Equine Safety For Diné Youth, Karah L. Shumway

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded through the National Children's Center for Rural and Agricultural Safety and Health as part of the pilot study program for 2011-2012 fiscal year. The project team proposed a one-year, $19,991 project to conduct a formative needs assessment of Diné parents for the prevention of agricultural injuries to children who are helping farm or ranch. The assessment utilized a survey constructed of closed and open ended questions to gauge Diné farmers' and ranchers' perceptions of injury risks to children who live or work on an agricultural operation. Additional questions were asked to gauge Diné acceptance of an …

Evidence Of Agrarian Urbanism: Land Use Preferences Of Residents Living On Small Acreage Farms Or Large Lots With Animal Rights In Cache Valley, Utah, Laurie B. Hurst

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Until the last half century, land development patterns in the Intermountain West were designed after the Mormon settlement pattern. With its gridiron streets and in-town farmsteads, this pattern gave families the opportunity to grow crops and raise a few animals on their one acre or less in town, with the added advantage of having a social life. In many places over the last century, cities have grown, small farms have dwindled, and large farms have been pushed to the fringe. However, in the Intermountain West the agrarian tradition continues with a number of families who grow gardens and raise animals …

Characterization Of Lactose Monolaurate For Its Antimicrobial And Emulsification Properties And Its Effect On Crystallization Behavior Of Anhydrous Milk Fat, Ashwini Wagh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A major class of synthetic emulsifiers used in food is sucrose esters. However, the use of lactose esters in foods is not very well known. Lactose monolaurate was characterized in our laboratory with respect to its antimicrobial, emulsification and fat crystallization modifier properties. The benefits of this study were multifold. A single compound was explored for multiple functionalities. Enzymatic synthesis and characterization of lactose monolaurate was carried out in the laboratory using food grade solvents. This process utilized lactose, a major byproduct of the dairy industry which is cheaper than sucrose.

In the first study, lactose monolaurate was explored for …

Forage Yield And Quality Of Binary Grass-Legume Mixtures Of Tall Fescue, Orchardgrass, Meadow Brome, Alfalfa, Birdsfoot Trefoil, And Cicer Milkvetch, Steven R. Cox

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Managed pasture forms the foundation for much of the U.S. livestock iv production. Increased forage yield and quality can be achieved with nitrogen (N) fertilizer but increases the cost of pasture production. Rising prices of N have led to a return to the use of grass-legume pastures to reduce or replace commercial N fertilizer. There is a need to identify viable grass-legume mixtures and species planting ratios for the region of the Intermountain Western United States The purpose of this study was to identify grass-legume combinations and planting ratios that maximize forage production and forage quality in irrigated pastures. The …

A Data-Intensive Assessment Of The Species Abundance Distribution, Elita Baldridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

One of the most commonly observed patterns in ecology is the fact that at most locations there are a large number of relatively rare species, composed of only a few individuals per species, and a small number of relatively common species. This pattern of commonness and rarity is quantified by the species abundance distribution. As one of the most commonly observed patterns in ecology, it has been studied intensively for over 100 years.

A major emphasis of this research has been developing models to try to understand the forces that generate such a general pattern. As a result, there are …

Evaluating Integrated Weed Management: Russian Knapweed Control With Goat Grazing And Aminopyralid, Clarke G. Alder

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Invasion of natural communities by introduced plants is considered one of the biggest threats to biodiversity. Weeds in rangelands cause an estimated loss of $2 billion per year in the United States. These costs include losses in forage quality and yield, grazing interference, animal poisoning, lowering land values, depleting soil water and resources available to native plants, increasing costs of managing livestock, and impacts on wildlife habitat and forage. Integrated weed management (IWM) is a way for land managers such as farmers, ranchers, and government agencies to control invasive weeds. IWM uses several different control methods working in conjunction to …