Life Sciences Commons^™

Effect Of Supplemental Prebiotics, Probiotics And Bioactive Proteins On The Microbiome Composition And Gut Physiology In C57bl6/J Mice, Ye Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system. The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice individually and in combination. Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and Tri-Factor (bioactive proteins) individually and in combination for four weeks. Subsequently, effects on the composition of gut microbiome, gut short chain fatty acids (SCFAs) concentration, gut …

Nest-Site Selection Of Golden Eagles And Ferruginous Hawks And Diet Composition Of Sensitive Raptor Species Using Metabarcoding Analysis In The Uinta Basin And Ashley National Forest, Ut, Usa, Dylan J. Hopkins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Development and climate change in the sagebrush habitats are causing population declines of North American hawks and eagles. For these species, understanding the landscape features that are preferred for nesting and the prey they consume in sagebrush habitats are important in developing conservation plans. Specifically, we know little of the preferred nest-sites and diet of Ferruginous Hawks (Buteo regalis) and Golden Eagles (Aquila chrysaetos) many locales. In our study, we determined the landscape characteristics associated with nest sites for these two raptor species in the Uintah Basin, UT to predict where nests may occur in our …

Quantifying The Predictability Of Evolution At The Genomic Level In Lycaeides Butterflies, Samridhi Chaturvedi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Stephen Jay Gould, a great scientist and evolutionary biologists, suggested that if we could replay the tape of life, we would not have observed similar course of events because evolution is stochastic and if affected by several events. Since then, the possibility that evolution is repeatable or predictable has been debated. Studies using large-scale evolution experiments, long-term data for individual populations, and controlled experiments in nature, have demonstrated phenotypic and genetic convergence in several taxa. These studies suggest that despite some randomness, predictable evolutionary patterns can emerge on a large temporal and spatial scale. However, a few cases also exist …

The Thermal And Physical Properties Of Beef From Three Usda-Quality Grades Cooked To Multiple Degrees Of Doneness, Jessica Mcclellan Hadfield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objective of this study was to determine the influence of quality grade (QG) and degree-of-doneness (DOD) on thermophysical properties of beef strip steaks. The “Prime” eating experience must be marketed to compete with cheaper protein sources, and so palatability is a major concern with beef products. Thermal and physical properties help shed light on the impacts various components have on beef palatability, mainly tenderness and juiciness. Warner-Bratzler shear force (WBSF) and the textural property of springiness are both influenced by a combination of QG and DOD. This is also true for viscoelasticity and the degradation of myosin and sarcoplasmic …

Evaluating The Effectiveness Of Livestock Guardian Dogs: Loss-Prevention, Behavior, Space-Use, And Human Dimensions, Daniel Kinka

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Livestock guardian dogs – or “LGDs” – are commonly used by domestic sheep ranchers and reduce the need for killing wild carnivores to protect livestock. LGDs are mostly used in the United States to reduce the number of livestock killed by coyotes, but whether they can prevent killing by larger carnivores like wolves and grizzly bears is unclear. It is important to identify which behavioral traits and LGD breeds work best for guarding livestock so that ranchers can protect their stock and environmentalists can enjoy a greater number of wild animals on the landscape. This study investigated the effectiveness of …

Explorations And Collaborations On Two Under-Recognized Native American Food Crops: Southwest Peach (Prunus Persica) And Navajo Spinach (Cleome Serrulata), Reagan C. Wytsalucy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Agricultural production among the Native American populations of the Southwest declined significantly during the twentieth century. Corn, beans and squash, the three most recognized traditional food crops, remains widespread, but knowledge regarding the traditional management of these crops was lost. The loss of traditional knowledge for Southwest Indigenous Nations was more pronounced for the Southwest peach (Prunus persica) and Navajo spinach (Cleome serrulata Pursh). The Navajo, Hopi, and Zuni Nations are all seeking to increase the availability of traditional crops for their original uses, such as for food and wool dye. In order to revitalize traditional agriculture …

Using Tree-Ring Growth And Stable Isotopes To Explore Ponderosa Pine Ecophysiological Responses To Climate Variability And The 2012-2015 California Drought, Rachel M. Keen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Climate warming in recent decades has resulted in more frequent and severe drought events in the western United States. These changes are projected to continue, making it exceedingly important to understand how forests respond to severe drought stress, and how we can manage these forests to reduce mortality during future events. The 2012-2015 California drought is a recent example of a severe, multi-year drought that was coupled with an epidemic-scale outbreak of western pine beetle, killing nearly 90% of ponderosa pines in the central and southern Sierra Nevadas. In the first portion of this study, we compared pairs of surviving …

Twenty-First Century Skills: A Needs Assessment Of School-Based Agricultural Education Teachers, Kisia J. Weeks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Preparing students to be career and work ready is a concern of educators and schools nationwide. Twenty-first century skills prepare students to enter the workforce or higher education with the ability to think critically and creatively, collaborate with others, take the initiative when approached with a task, and use technology to its fullest potential. If students are not learning the skills needed for success, it is because educators and schools are not teaching them. When students possess these skills, they are prepared to work in teams, think critically and creatively about a problem, display leadership and social skills, and communicate …

Linkage Of Climate Diagnostics In Predictions For Crop Production: Cold Impacts In Taiwan And Thailand, Parichart Promchote

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research presents three case studies of low temperature anomalies that occurred during the winter–spring seasons and their influence on extreme events and crop production. We investigate causes and effects of each climate event and developed prediction methods for crops based on the climate diagnostic information. The first study diagnosed the driven environmental-factors, including climate pattern, climate change, soils moisture, and sea level height, associated with the 2011 great flood in Thailand and resulting total crop loss. The second study investigated climate circulation and indices that contributed to wet-and-cold (WC) events leading to significant crop damage in Taiwan. We developed …

Eared Grebe Nesting Ecology And Chronology Along The Great Salt Lake, Utah, Leah M. Delahoussaye

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eared Grebes (Podiceps nigricollis) are migratory birds that build their nests over water and in large groups called colonies. Their typical breeding range is in central southern Canada and northern United States; however, a previously uncertain number of Eared Grebes (grebes) also nest around the Great Salt Lake (GSL), Utah, at the southern edge of their breeding range. Little is known about the habitat requirements for grebe nesting colonies at such low latitudes and if they are different from colonies found elsewhere. My objectives for this research were to determine the status of the grebe nesting population as …

Barriers, Roles, And Information Source Preferences For Utilizing Agricultural Science Projects Among Utah 4-H Extension Professionals, Aleigh Aurin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Agriscience is the growing study of biotechnology, business, and economics within the agriculture industry. Through 4-H, youth can experience hands on learning through different program areas. The purpose of this study was to identify the barriers, roles, and how Utah 4-H personnel preferred to learn about new programs. The study showed time and available volunteers were a barrier for 4-H Extension personnel to implement Agriscience projects. Other results included internet and workshops as the top choices for training programs. The Extension personnel agreed it was their job to involve and educate youth. Yet, need training in how to integrate Robotics; …

Electronic Transmutation: An Aid For The Rational Design Of New Chemical Materials Using The Knowledge Of Bonding And Structure Of Neighboring Elements, Katie A. Lundell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Everything in the universe is made up of elements from the periodic table. Each element has its own role that it plays in the formation of things it makes up. For instance, pencil lead is graphite. A series of honeycomb-like structures made up of carbon stacked on top of one another. Carbon’s neighbor to the left, boron doesn’t like to form such stacked honeycomb-like structures. But, what if there was a way to make boron act like carbon so it did like to form such structures? That question is the basis of the electronic transmutation concept presented in this dissertation. …

Genetic Mapping Of Grass Monoculture And Grass-Legume Mixture Compatibility Qtls In Intermediate Wheatgrass, John Mortenson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Due to increased environmental stewardship and fertilizer prices, there is increased interest in using legume mixes in perennial croplands. The objective of this study was to compare quantitative genetic parameters and quantitative trait loci (QTLs) associated with intermediate wheatgrass (Thinopyrum intermedium) when grown in 1) a non-competitive spaced environment, 2) a polyculture with alfalfa (Medicago sativa), and 3) a monoculture with crested wheatgrass (Agropyron desertorum). Traits evaluated include plant growth characteristics (Zadok’s maturity, height, and tiller count), biomass, and forage nutritive value (CP, NDF, ADF, ADL, IVTD, NDFD, NFC, ME, RFQ). A linkage map …

Investigation Of The Molecular Determinants And Extrinsic Factors That Regulate Prmt Product Specificity, Tamar B. Cáceres

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protein arginine methylation is an important modification of proteins, involved in many cellular processes. Some examples are transcription, RNA editing, cellular communication, DNA repair, viral replication and chromatin remodeling. In recent years, the significance of protein arginine methyltransferases (PRMTs) in human diseases has been increasingly studied, especially in cardiovascular disease and cancer. Although the importance of these enzymes is recognized, the understanding of how exactly PRMTs function is still limited. Very little information is available to explain how or why any of the different PRMTs interact with other proteins or, what determines where in that protein to place their methyl …

Ecosystem Functioning Of Great Salt Lake Wetlands, Maya Cassidy Pendleton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Great Salt Lake (GSL) wetlands account for ~75% of all Utah wetlands and provide not only critical habitat for millions of migratory birds, but also provide valuable ecosystem functions and services as well as economic benefits to Utahns. However, these wetlands are facing an aggressive invader, Phragmites australis, that has spreading across the GSL wetlands and replacing native wetland habitats. Wetland managers have spent countless resources and time trying to control the spread of P. australis and restore GSL wetlands. However, we do not fully understand how these wetlands functions and services are being altered with this habitat …

A Universal Mechanism For N2 Binding To And Differing Reactivity Of The E4(4h) State Of Mo-, V-, And Fe-Nitrogenases, Derek Franklin Harris

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As a basic building block in many biological molecules, the element nitrogen (N) is essential for life. Dinitrogen (N₂) is abundant in Earth’s atmosphere, but this form is biologically unavailable. To be biologically available, N₂ must undergo a reduction reaction to the fixed form, ammonia (NH₃). The industrial Haber-Bosch process, which accounts for approximately 50% of the worlds fixed nitrogen, uses energy from fossil fuels to achieve high pressures and temperatures to catalyze the reaction. The energy used by Haber-Bosch accounts for approximately 2% of the world’s annual supply. The remainder of fixed nitrogen is …

Epigenetic Reprogramming, Apoptosis, And Developmental Competence In Cloned Embryos, Laura A. Moley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after the first demonstration of the technology with the birth of Dolly. By increasing efficiency by selecting the embryos early in development that are most likely to succeed following transfer into a surrogate mother, the technology could be more routinely utilized to enhance animal agriculture production. SCNT is believed to be highly inefficient as a result of incorrect DNA methylation and gene expression that are accumulated because of the SCNT technique. We proposed the use of a non-toxic, non-invasive detector of cell death, to quantitatively assess embryo competency …

The Vulnerability Of Littoral Structures Under Multiyear Drought Conditions, Jenna M. Keeton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Climate change is associated with altered environmental conditions and shifting mosaics of suitable habitats for organisms. Climate change in the form of drought can shift important lake shoreline habitats downslope, altering the lakes chemistry and habitat availability. Additionally, negative biological consequences can occur after a loss of submerged habitat along shorelines, hereafter littoral habitat. The objective of this study is to evaluate whether littoral habitat is lost (cobble, coarse woody habitat (fallen trees; CWH), and aquatic vegetation) under drought conditions across the United States. I used the National Lakes Assessment physical habitat data collected in summer 2012, when 75% of …

The Effect Of Lactation And Energy Status On Gene Expression In The Main Reproductive Tissues Of Lactating Dairy Cattle, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Modern high-yielding dairy cows are currently producing far more milk than their ancestors due to a prolonged and intensive genetic selection for milk production trait accompanied by the revolutionary improvement in technology, management, and nutrition. On the other hand, a noticeable decline in fertility and reproductive performance was undeniably consistent with the increase in milk yield. This decline in fertility and reproductive performance are recognized worldwide and well documented in several studies. Dairy cows typically experience a period of energy deficit during the first few months of lactation due to the rapid increase in milk production and limited feed intake. …

Biophysical And Structural Characterization Of Shigella Atpase Spa47 Oligomerization Provides Insight Into Type Three Secretion System Activation And Virulence, Jamie L. Burgess

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several bacterial pathogens including Shigella (shigellosis), Escherichia coli (urinary tract infections), Pseudomonas (lung infections), Salmonella (food poisoning), and Yersinia (plague) critically rely on a complex type three secretion system (T3SS) for infection. With the rise in multi-antibiotic resistant strains of several of these pathogens, we turn to the T3SS as a promising target for the development of novel therapeutics. The Dickenson lab at Utah State University has been the first to identify and characterize the ATPase Spa47, the energy source of the Shigella infection system. We show that Spa47 is necessary for proper T3SS formation and function, being ultimately responsible …

Influence Of Change In Ph On Whey Expulsion From Cheddar Cheese Curds Made From Recombined Concentrated Milk, Kanak Bulbul

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Western Dairy Center at Utah State University funded this project to investigate cheese research using concentrated milks. Concentrated milk was provided by the South Dakota State University and starter culture for this study was prepared and donated by Vivolac Cultures Corporation, Greenfield, Indiana.

The project initiated as a continuation of a previous study on effects of protein concentration, coagulum cut size and set temperature on curd moisture loss kinetics while stirring during cheesemaking. It was aimed at determining the extent to which pH drop prior to draining and final cheese moisture when using microfiltered concentrated milk.

We performed twelve …

The Spatial Ecology Of Predator-Prey Interactions: A Case Study Of Yellowstone Elk, Wolves, And Cougars, Michel T. Kohl

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The loss of large apex predators, and their subsequent reintroduction, has been identified as a substantial driver on the structure and function of ecological communities through behavioral mediated trophic cascades (BMTCs). The reintroduction of wolves (Canis lupus) to Yellowstone National Park (YNP) has served as foundational case study of BMTCs. In our system, it has been suggested that wolves have established a ‘landscape of fear’ in which the primary prey, elk (Cervus elaphus), now avoid risky places, which ultimately led to the recovery of the vegetation community. Although this case is frequently cited as a well-understood …

Evaluating Michigan's Food, Agriculture, And Resources In Motion (Farm) Science Lab As A Modality For Agricultural Literacy, Amelia J. Miller

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Michigan’s Food, Agriculture, and Resources in Motion (FARM) Science Lab is a 40-foot mobile classroom outfitted with 10 learning stations including scientific equipment and iPads. This quasi-experimental study analyzed preexisting data provided by Michigan Agriculture in the Classroom to evaluate the effectiveness of the FARM Science Lab mobile classroom as a method of teaching agriculture-themed, standards-based lessons to third- through fifth-grade students in order to increase their understanding of agriculture. From January through June 2018, more than 1,258 students participated in these lessons and completed the pretest and posttest and 72 teachers completed the post survey. Research questions not only …

Untangling The Economic And Social Impediments To Producer Adoption Of Organic Wheat, Donya L. Ralph-Quarnstrom

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumer demand for organic products has shown double-digit growth in recent years encouraging the development of a wider range of goods (Greene, 2017). Americans with an annual household income under $30,000 actively purchase organic foods at nearly the same rate as households with over $75,000 in annual incomes, 42% versus 49% (Greene et al., 2017). Previous research observed the adoption of organic farming practices on a combination of different grains, fruits and vegetables, meat, and dairy products from across the globe. However, this is the first study to examine the adoption of organic wheat in the Western U.S. By addressing …

Impacts Of Cattle Grazing As A Tool To Control Phragmites Australis In Wetlands On Nitrogen, Phosphorus, And Carbon, Brittany L. Duncan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Phragmites australis is a plant that is causing problems in wetlands by outcompeting native plants that provide food and shelter for millions of migratory birds. Currently, managers try to control Phragmites australis by spraying herbicide, burning, and mowing, but these methods are costly, time consuming, and have low levels of success. Adding grazing as a tool to control Phragmites australis provides a cheap and low labor alternative. However, there are many concerns regarding if grazing will cause nutrient loading in our wetlands that will decrease water quality and alter beneficial functions of wetlands.

To better understand the effects of grazing …

Development Of Mouse Models For Respiratory And Neurological Disease Caused By Enterovirus D68 And Evaluation Of Antiviral Therapies, Brett L. Hurst

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Enterovirus D68 (EV-D68) is a virus that normally causes disease in children. While this virus typically causes a respiratory infection, in 2014, a large outbreak of the virus was associated with patients that had paralysis of the arms or legs. Even though the virus was discovered in 1962, little was known about the life cycle of the virus or its ability to cause disease. An animal model of disease was needed to understand how the virus causes disease and to develop antiviral compounds to target the virus life cycle.

We adapted the virus by serial-passage in lung tissues from mice …

Landscape Of Stress: Does Drought Prevail Over Anthropogenic Activity In Influencing Cortisol Levels And Fitness In The Pacific Fisher?, Jennifer R. Kordosky

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fishers (Pekania pennanti) are a species of concern in the Sierra Nevada Mountains of California. Cortisol is a glucocorticoid hormone released to mobilize energy in response to stress and has been used as an indication of an individual’s physiological response to its environment. By collecting samples of fisher hair and measuring an individual’s cortisol, we examined the physiological stress response of the animals to human disturbances (housing density, road density, habitat type, and silvicultural treatements) and drought (tree mortality) in their home ranges. Using AICc model selection, we found that levels of tree mortality within a fisher’s home …

Latino Stepcouples’ Experiences Two Years After Stepfamily Education And A Narrative Study Regarding Changes In A Latino Stepfamily Two Years After Stepfamily Education, Sheryl Ann Goodey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

There are two studies in this dissertation. Both are about couples in a stepfamily who attended stepfamily education called, Smart Steps: Embrace the Journey. The first study explored how 13 Latino stepcouples felt the concepts they learned in stepfamily education influenced their couple relationship, parenting, and their family. Participants ranged in age from 25 to 43. They were interviewed two years after participating in the course. They talked about positive changes that happened in their couple relationship, parenting relationship, and their family as a result of participating in stepfamily education. It was also found that the course helped them …

Ruminal Protection And Intestinal Availability Of Rumen-Protected Methionine And Lysine In Lactating Dairy Cows, Sara Menchu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Rumen protected Methionine (MET) and Lysine (LYS) are critical for milk protein synthesis in dairy cows. N-acetyl-L-methionine (NALM) is a MET derivative that consists of L-Met protected with an acetyl group that is attached to the α-amino group.N-acetyl-L-lysine (NALL) is a LYS derivative that is similarly protected. The objectives of these studies were to quantify the gastrointestinal availability of NALM and NALL. Three experiments were run as 3 × 3 Latin square using 3 second lactation Holstein cows that have been fitted with cannulas in the rumen and duodenum. The cows …

The Total Western Diet And Vancomycin Treatment Increase Inflammation-Mediated Colorectal Cancer, Niklas David Joakim Aardema

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Prior work by our research group showed that the total Western diet (TWD), a rodent diet which models the typical American diet, promoted the development of colon tumors when fed to mice. Other researchers previously showed that vancomycin, an antibiotic that changes the gut microbiome composition, causes differential changes in the severity of colon inflammation and CRC. Our goal was to determine the combined effects of feeding the TWD and vancomycin treatment on colitis and CRC, and if these factors interact. We hypothesized that vancomycin treatment would mitigate colitis and CRC in mice fed the TWD. To this end, mice …