Life Sciences Commons^™

Effects Of Premortem Stress On Heat Shock Protein Abundance, Oxidation, And Color In The Longissimus Lumborum Of Holstein Steers Following Harvest, Reganne K. Briggs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumers consider tenderness and steak color to be two important attributes associated with meat quality. However, inconsistencies in both tenderness and steak color remain despite similar production practices of beef cattle. Stressful events before harvest may impact meat quality by initiating certain pathways such as abundance of heat shock proteins (HSP) and oxidation within the skeletal muscle. Heat shock proteins have been associated with tenderness while oxidation may affect steak color and flavor. In this study, 40 Holstein steers were administered adrenocorticotrophic hormone (ACTH) to mimic a stressful event before harvest. Animals were harvested at different times following the ACTH …

Propagation Of Two Utah Native Plants: Ceanothus Velutinus And Cercocarpus Montanus, Asmita Paudel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Among various water conservative approaches, the use of native plants in landscape, such as Ceanothus velutinus (snowbrush ceanothus) and Cercocarpus montanus (alder-leaf mountain mahogany), is attractive. Efficient propagation methods are required to allow these native species to use in water-efficient landscaping. Sexual (seed) and asexual/vegetative (cuttings and micropropagation) propagation methods were evaluated.

Seeds of both C. velutinus and C. montanus were scarified and/or stratified and treated with gibberellic acid (GA₃) to break dormancy. The results showed hot water scarification and 2-3 months of stratification effectively broke the dormancy of C. velutinus seeds, and stratification for 2-3 months was …

Response Of Glyphosate-Resistant Alfalfa To Glyphosate Application In The Intermountain West, Logan Chet Loveland

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Glyphosate-resistant (GR) alfalfa (Medicago sativa L.) has been widely adopted in the Intermountain West United States, where alfalfa plays an important role in agriculture. Exceptional tolerance to glyphosate application has been a reported strength of this technology; however, growers have recently reported potential crop injury under certain environmental conditions. The purpose of this study was to document and characterize the injury, identify local conditions that may have contributed to crop injury, and determine best management practices for avoiding injury to GR alfalfa in the Intermountain West. The effects of glyphosate rate and application timing were investigated at 24 sites …

A Data-Driven Regional Model Of Stomatal Conductance For Kruger National Park, Rebecca L. Tobin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Stomata are the gateway between the lithosphere, the biosphere, and the atmosphere. Because of photosynthesis, plants inevitably lose water through their stomata. The rate at which water moves through stomata is stomatal conductance. As stomatal conductance increases, the rate of CO₂ assimilation increases, therefore, plants must reach a balance between acquiring CO₂ and losing H₂O. Plants achieve this balance by adjusting stomatal aperture. Therefore, modeling stomatal conductance is important to global circulation models and land surface models, as well as for predicting how changing climate conditions affect water use efficiency and plant productivity, and has implications …

Impacts Of Tourism On The Ecophysiology Of The Endangered Northern Bahamian Rock Iguana (Cyclura Cychlura), Alison C. Webb

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increased interest in ecotourism over recent years has led to more direct human-animal interactions and a striking concomitant increase in the provisioning of non-natural food, that may have unintended consequences for the wildlife involved. The critically endangered Northern Bahamian Rock Iguana provides a valuable model to address the potential impact of food provisioning on health as there are populations that represent a graded variation in human presence, with sites experiencing high, low, or no tourism. To assess the potential impacts of tourism on iguana physiology I first reviewed the relevant literature on iguana physiology and then performed three investigations focusing …

Reusable Light-Activated Antimicrobial Materials Prepared With Food Ingredients, Andrew T. Gagon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Microbial contamination and proliferation in food processing, distribution and storage continues to be a real risk in spite the wide variety of food processing and preservation techniques available. Some of these techniques (such as heat treatments and the use of chemical preservatives) may require substantial amounts of energy and their effects in the general population and the environment may be harmful. This has generated an interest over alternative methods of food preservation. The use of reusable light-activated materials may represent an effective approach. In this study, a polypropylene plastic was modified with either zein, which is found in corn, or …

The Effects Of Different Organic Pastures On Dairy Heifer Growth And Development, Jacob A. Hadfield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Raising dairy heifers in a certified organic setting can be difficult for producers. Conventionally, heifers are raised in a confined setting, and fed a total mixed ration (TMR) that is balanced daily to contain all the needed nutrients for developing heifers. Organic producers can use a TMR in their operations, but due to high organic feed costs, many choose to raise their heifers in pasture-based systems. While pasture-based systems may lower costs, heifers on pasture commonly have lower rates of gain, which can be financially burdensome to producers. Grass-legume pastures may help improve rates of gain in heifers on pasture-based …

New World Mammarenaviral Hemorrhagic Fever Virus Determinants Of Infection And Disease, Brady T. Hickerson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several New World mammarenaviruses (NWM) are able to cause life-threatening hemorrhagic disease in humans. These agents use human transferrin receptor 1 (hTfR1), the main protein involved in cellular uptake of iron, to infect cells. Furthermore, use of hTfR1 correlates with the ability of these viruses to cause severe disease in humans. The most prevalent pathogenic NWM is Jun√≠n virus (JUNV), which is the etiological agent of Argentine hemorrhagic fever (AHF).The pathogenesis of AHF is poorly understood. A cardinal feature of severe cases of AHF is a prolonged increase of serum interferon-a (IFN-a) concentration. This increase is hypothesized to play a …

Influence Of Forage Diversity And Condensed Tannins On Livestock Foraging Behavior, Production And Environmental Impact, Sebastian P. Lagrange

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eating a combination of forages with different chemistries (i.e., nutrients, beneficial compounds such as tannins) may enhance ruminant nutrition and reduce environmental impacts relative to eating single forages. I explored the influence of offering sheep and cattle all possible combinations of tanniferous (i.e., plants with tannins; birdsfoot trefoil, sainfoin) and non-tanniferous legumes (i.e., plants without tannins; alfalfa) or their monocultures on animal performance, behavior, and methane and nitrogen (N) emissions. Offering choices among these legumes to penned sheep improved intake and diet digestibility relative to feeding monocultures. Mixtures selected by sheep were better digested than mixtures containing equal parts of …

Improving Our Ability To Estimate Vital Rates Of Endangered Fishes On The San Juan River Using Novel Applications Of Pit-Tag Technology, J. Benjamin Stout

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Estimating demographic parameters, such as survival and abundance, with accuracy and precision is vital for detecting trends in populations and assessing the effectiveness of management actions. In most cases, a lack of capture data make estimating parameters very challenging. The use of new technologies to increase the amount of remotely collected data is increasing, but brings new limitations and analytical issues to be resolved. One of those new technologies is the use of a mobile floating PIT-tag antenna to detect PIT-tagged fish. The issue that arises with this technology is determination of the status of detected tags (i.e., live fish …

The Effects Of Gene Therapy In An Ovine Osteoarthritis Model, Crystal Collier

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Humans rely on the health of their joints for stability and mobility in daily life. In a normal, healthy functioning joint, complex processes maintain joint tissues, including remodeling, lubrication, and immune function among many other tasks. Osteoarthritis (OA) is the most common joint disorder in the U.S. It becomes most prevalent in adults 60+ years of age and causes decreased mobility, discomfort, and in some cases, excruciating pain. In the biological processes of osteoarthritis, the metalloproteinase enzymes responsible for the degeneration of cartilage are upregulated. The inhibitors of metalloproteinase activity are known as Tissue Inhibitor of Metalloproteinases (TIMPs), which keep …

Comparative Analysis Of Small Non-Coding Rna And Messenger Rna Expression In Somatic Cell Nuclear Transfer And In Vitro-Fertilized Bovine Embryos During Early Development Through The Maternal-To-Embryonic Transition, Jocelyn Marie Cuthbert

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cloning animals using somatic cell nuclear transfer (scNT) was first successfully demonstrated with the birth of Dolly the sheep, but the process of cloning remains highly inefficient. By improving our understanding of the errors that may occur during cloned cattle embryo development, we could obtain a greater understanding of how specific molecular events contribute to successful development. The central dogma of biology refers to the process of DNA being transcribed into messenger RNA (mRNA) and the translation of mRNA into proteins, which ultimately carry out the functions encoded by genes. The epigenetic code is defined as the array of chemical …

Ecology And Economic Impact Of The Invasive Brown Marmorated Stink Bug (Hemiptera: Pentatomidae; Halyomorpha Halys) In The Utah Agricultural Landscape, Zachary R. Schumm

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The brown marmorated stink bug (BMSB) is a major insect pest that causes economic loss to a diversity of U.S. fruit and vegetable crops, and invades homes and human structures, causing nuisance issues for homeowners. This destructive insect causes millions of dollars of crop damage annually, and is difficult to manage due to its resistance to some common insecticides. BMSB is a relatively new pest to Utah, and its biology and ecology is not well known in the high elevation, arid Intermountain West region. In Chapter II, I explored the potential impact of BMSB to tart cherry, an unstudied crop …

Enhancing The Production And Sustainability Of Pasture-Fed Beef Using Non-Traditional Legume Forages, Andrea I. Bolletta

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Despite the increasing worldwide demand for beef as a protein source, consumers are concerned about the sustainability of ruminant production systems. Their main concerns are animal welfare for feedlot-fed animals, greenhouse gas (GHG) emissions, global warming and worker safety. Traditional feedlot-based beef production systems have been associated with locally greater levels of soil, water and air contamination, as well as the overuse of antibiotics and growth hormones. The use of legume pastures such as cicer milkvetch (CMV) and birdsfoot trefoil (BFT), which fix their own nitrogen (N) and often contain beneficial secondary compounds such as tannins and provide for rapid …

Investigating Nest Box Utilization By Bumble Bees And Reproductive Development Of Male Bumble Bees, James Daniel Herndon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bumble bees (Bombus Latrielle) are a wide-spread group of pollinating insects that are important species to conserve across many environments to ensure both ecological and economic resiliency because they pollinate important agricultural, horticultural, and wild flora. Surprisingly, fundamental questions still remain about this important charismatic group of pollinators. The investigations in this thesis are but two of many topics that require further research. The topics investigated are understanding bumble bee nest site preferences and reproductive development of both sexes of bumble bees. The first experiment (Chapter 2) investigates whether nest boxes elevated off of the ground and attached to …

The Effects Of Milk Phospholipids On Functional Properties Of Anhydrous Milk Fat And Oil Structured Gel Systems, Zachary Cooper

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lecithin is an ingredient commonly used in foods composed of high concentration of phospholipids. Phospholipids are a type of fat that can mix with both water and oil. In the current food industry, most lecithins come from sunflower, egg, and soybean sources. An additional source of phospholipids can be the waste streams from the dairy industry, such as the whey protein and butter making processes, since these contain a high concentration of phospholipids. Dairy phospholipids have been isolated in the past, but their traits as a functional ingredient are still unknown. In addition, most isolated dairy phospholipids still contain protein, …

Interactions Between Water-Stress And Maize Resistance To Spider Mites With Varied Host Specialization, Gunbharpur S. Gill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider mites are well-documented pests of many agricultural crops including cereals such as maize. In hot and dry conditions, when crop plants are under water-stress, spider mite populations can rapidly increase within weeks and can cause severe yield losses. One approach to manage pests, such as spider mites, is through the development and selection of plants that can resist pests. Screening of maize lines has provided evidence of spider mite resistance; however, it is unclear how plant water-stress may affect the ability of maize to sustain spider mite resistance. In addition, it is important to understand how plants respond to …

Exploring The Connection Between Acid Exposure And Virulence In Listeria Monocytogenes, Minghao Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Listeria monocytogenes is a gram-positive food-borne pathogen that is widely dispersed in the environment and can cause listeriosis with high fatality rates when consumed in contaminated food products. They are capable of growing over a wide range condition. Listeria is also able to tolerate adverse conditions which allows the bacterium to survive in unfavorable environments. The ubiquitous nature of L. monocytogenes makes it difficult to eliminate from food systems. One major problem in the food industry is the survival of L. monocytogenes under sublethal low pH-environment since organic acids are widely used as food decontaminants. Prior research has suggested that …

Harmful Algal Blooms: Dominance In Lakes And Risk For Cyanotoxin Exposure In Food Crops, Austin D. Bartos

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Climate change and human activities are promoting the dominance of a photosynthetic family of aquatic bacteria, cyanobacteria. Blooms of cyanobacteria are not only a visual nuisance but can produce a variety of cyanotoxins than can harm the liver, skin, and nervous system of animals and humans. We analyzed lakes in the contiguous United States and found that between 2007 and 2012, the number of lakes that produced measurable quantities of cyanotoxins increased from 33% to 45%. Nitrogen and phosphorus pollution were the main drivers of cyanobacteria blooms and toxin production between these years. Many of these lakes and reservoirs are …

Exploring In Vivo And In Vitro Regulation Of The Shigella Type Three Secterion System Atpase Spa47, Heather B. Case

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Diarrheal disease is caused by a diverse group of enteric pathogens and is one of the leading causes of preventable deaths worldwide. Some of the most common causes of diarrheal disease are bacterial pathogens such as Salmonella, E. coli, Cholera, and Shigella. Many bacterial enteric pathogens use a type three secretion system (T3SS) to cause infection. The T3SS uses a needle and syringe like apparatus that allows the bacteria to inject proteins into the host cell to cause infection. Striking similarities in T3SSs from all pathogens that utilize them makes the T3SS an attractive target for …

Harvest Efficiency Of Forage Grazed By Cattle And The Effect Of Pinyon And Juniper Treatments On Vegetation Cover On The Grand Staircase Escalante National Monument, Ruger P. Carter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Grand Staircase Escalante National Monument (GSENM), located in central south Utah, currently has 76,957 active grazing animal unit months on the monument. Recently, there has been questions whether the harvest and grazing efficiency coefficients developed in the Midwest are applicable to the arid, bunch grass dominated systems of the GSENM. Harvest and grazing efficiency defines the percentage of allocated forage that is being ingested by the animal, and the percentage that is being wasted. Harvest and grazing efficiency coefficients were calculated on the Lower Cattle allotment on the GSENM by taking total forage production and dividing that by expected …

Connections Between Hydrothermal System Geochemistry And Microbiology: Traversing Tectonic Boundaries In The South-Central Peruvian Andes, Heather Upin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Geochemistry and microbiology are inherently tied in the natural world. The study of geomicrobiology has historically taken place in extreme systems, like hot springs of Yellowstone National Park and deep-sea hydrothermal vents, because the organisms that exist there have deep lineages on the tree of life and provide insight into early life on Earth. These microbes use chemical energy from nutrients available in their environment rather than relying on photosynthesis, energy obtained from the sun, to support their metabolism. The goal of this study is to improve our understanding of geological controls (for example the tectonic setting) on hot spring …

Anthropogenic Influences On Bacterial Assemblages In Stream Biofilms, Elizabeth M. Ogata

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bacteria within biofilms are an essential component of stream ecosystems, influencing the movement of carbon (C), nitrogen (N), and phosphorus (P) in watersheds. To better understand the ecological effects of human activities on stream ecosystems, my research examined how nutrients and pharmaceuticals, common pollutants in streams worldwide, influence bacterial assemblages in stream biofilms. First, I tested how nutrients (N, P, iron) and pharmaceuticals (caffeine, diphenhydramine) influenced biofilm bacterial microbiomes (taxa present in at least 75% of samples of a contaminant treatment). Nutrients allowed taxa known for their ability to thrive in nutrient-rich environments to dominate microbiomes, pharmaceuticals supported a rich …

Improving Aquatic Habitat Representation In Utah Using Large Spatial Scale Environmental Datasets, Gregory C. Goodrum

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Rivers provide habitat for aquatic species, but widespread human water development degrades aquatic habitat, fragments stream networks, and threatens native fish populations. Habitat suitability models are commonly used to identify current instream habitat conditions, but are often species-specific, data-intensive, and rarely suitable to the large spatial scales required in conservation and water resources management. Thus, there is need to develop and validate habitat suitability models that provide ecologically-meaningful estimations of aquatic habitat, but are simple enough to apply at large geographic areas and flexible to incorporate different species. I tested the accuracy of 15 habitat suitability models estimating Bonneville Cutthroat …

Effect Of Long Pasteurization Run Times On Bacterial Numbers In Milk, Brynli Tattersall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the Western Dairy Center to understand how long a milk pasteurizer can be operated before increases in bacterial numbers are observed in the pasteurized milk. While pasteurization kills pathogenic bacteria there are some non-pathogenic bacteria that can survive and have the ability to become attached to the surfaces in the cooling sections of the pasteurizer. Some bacteria can also produce spores that survive pasteurization even if the bacterial cells are killed. Temperatures in the cooling section remain in a range suitable for growth of these heat-tolerant bacteria and can allow germination of bacterial spores. While …

Ecoacoustic Methods For Multi-Taxa Animal Surveys In The Amazon, Leandro A. Do Nascimento

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tropical regions host most of the biodiversity found on Earth, but these species-rich areas are constantly threatened by human development and other disturbances that put this diversity of life forms at risk. To avoid extirpations of animal and plant species, scientists and managers rely on accurate monitoring techniques to retrieve information about population trends. This task is not easy, especially in the tropics, where there is often a lack of personnel to conduct surveys, a lack of funding, and the areas are so extensive that many countries need to be involved in monitoring (e.g., Amazon biome). For this reason, scientists …

An Evaluation Of Arthropod Assemblages In Great Salt Lake Wetland Habitats: Differences Between Native And Invasive Vegetation And Implications For Restoration, Emily E. Leonard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wetlands provide important habitat for various birds. Invasive plants can disrupt wetland food webs by altering the arthropod assemblages (invertebrate animals such as spiders, mites, insects, centipedes, and millipedes) on which these birds rely. However, differences between the wetland arthropods found in invasive vs. native vegetation are poorly defined. Wetlands are often managed for the creation of bird habitat through invasive species removal and native plant revegetation, yet few studies have examined the effects of these restoration methods on arthropod bird food sources. Phragmites australis (common reed), is an aggressive grass species in wetlands surrounding the Great Salt Lake, Utah, …

Forest Grouse Ecology And Management In The Bear River Range Northern Utah, Skyler Y. Farnsworth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To better manage dusky grouse (Dendragapus obscurus) and ruffed grouse (Bonasa umbellus), hereafter forest grouse, managers require better information on forest grouse population status and habitat selection. To address this need, from 2015-2017, I conducted research on a sympatric populations inhabiting the Bear River Range of northern Utah to develop a breeding survey protocol, assess habitat selection, evaluate dusky grouse response to livestock grazing, and determine hunter harvest rates.

The breeding census protocol that I developed compared listening intervals with and without electronic playback calls at designated survey stop locations. Using digital mapping software, I plotted …

Interspecific Differences In Stressor Tolerance Drive Community-Level Changes In A Small Aquatic Ecosystem, Jennifer M. Weathered

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The global human population is expected to reach 9.8 billion by 2050 and an increase in food yield will be needed to provide for the future generations. Insects are among the biggest threats to food production and are therefore the subject of intense chemical control through the application of pesticides. The ability of insects to evolve resistance to pesticides after repeated use has been documented. However, how evolved responses affect individuals’ behaviors, their interactions with others, and how these factors impact overall patterns in distribution remains relatively unexplored in the bromeliad.

The aquatic insect communities housed in water-holding tropical plants …

The Utility Of Environmental Dna And Species Distribution Models In Assessing The Habitat Requirements Of Twelve Fish Species In Alaskan North Slope Rivers, James B. Eddings

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Subsistence fishing is a vital component of Alaska’s North Slope borough economy and culture that is being threatened by human disturbance. These threats mean the fish must be protected, but the size of the region makes conservation planning difficult. Fortunately, advances in species distribution models (SDMs), environmental DNA (eDNA), and remote sensing technologies provide potential to better understand species’ needs and guide management. The objectives of my study were to: (1) map the current habitat suitability for twelve fish species, occurring in Alaska’s North Slope, (2) determine if SDMs based on eDNA data performed similarly to, or improved, models based …