Life Sciences Commons^™

The Two-Way Mixed Model Analysis Of Variance, Kenneth Davis Buckley

Theses and Dissertations

The analysis of variance for experiments where the fixed effects or random effects model is appropriate is generally agreed upon with regard to testing procedures and covariance structure. It is only in experiments involving both random and fixed factors, i.e. mixed effects models, that controversy occurs as to the proper analysis. The mixed effect model has been considered by many statisticians, and several techniques have been developed for explaining its structure and performing its analysis for balanced data sets. The relationship of these techniques have been discussed in several papers as well.

The simplest case of the difficulties presented by …

Hepatic Ornithine Metabolism In Hydrazine-Treated Rats, Van Saxton Hubbard

Theses and Dissertations

Ornithine plays an important role in mammalian intermediary metabolism. Ornithine can be (1) utilized as a component of the urea cycle, (2) decarboxylated to form putrescine, a precursor of the polyamines, spermidine and spermine, and (3) converted metabolically to arginine, proline and glutamate. In prior investigations conducted in our laboratories and others, free endogenous hepatic pools of ornithine were found to be elevated following hydrazine administration. Consequently, time-course alterations of arginase, ornithine δ-transaminase, ornithine transcarbamylase, and ornithine decarboxylase activities were investigated regarding the effect of hydrazine treatment on hepatic ornithine metabolism in the rat. As an outgrowth of these investigations, …

The Effect Of Aflatoxin B1 On Growth Rate And Iron Metabolism In Juvenile Mongolian Gerbils (Meriones Unguiculatus), William Stanley Hastings Jr.

Theses and Dissertations

The metabolic secretions from Aspergillus flavus, aflatoxins. are often found as contaminants of agricultural products used for human and animal consumption. Aflatoxin B1 is the most toxic and carcinogenic of the aflatoxins. A reduction in weight gain has been observed when juvenile animals are fed aflatoxin B1 over an extended period of time. This reduction in weight gain may possibly result from an anemic condition induced by an alteration of iron absorption and/or distribution. The effect of chronic sublethal levels of this hepatocarcinogen on weight gain and the uptake and distribution of 59Fe++ were studied in this investigation.

An experimental …

Studies Of Teratogenic Viruses: Minute Virus Of Mice And Colorado Tick Fever Virus, Robert Eldred Harris

Theses and Dissertations

Studies were conducted in DUB/ICR mice with minute virus of mice (MVM), a single stranded DNA virus, and Colorado tick fever (CTF) virus, a double stranded RNA Virus, to determine if CTF virus had teratogenic potential and to characterize further MVM and its teratogenic effects. MVM is a known teratogen, whereas CTF virus had never been investigated previously for teratogenic potential.

In characterization studies of MVM infectivity, the following were determined: (a) Rat embryo cells proved to be superior to six cell lines in assaying for MVM infectivity; (b) MVM infectivity could be inactivated with common laboratory disinfectants, ultraviolet radiation, …

The Serlogical Specificity Of The Lectin From Lens Culinaris, Deborah Ward Heritage

Theses and Dissertations

Lens culinaris, the common lentil, contains a lectin which has been shown to be specific for a glycoprotein saliva antigen and a glycolipoprotein serum antigen. Both the saliva and serum precipitin reactions with the lectin are directly inhibited with saccharides, especially those related to D-mannose. Electrophoresis of the serum antigen showed that it migrates as three bands, while appearing as a single band in double diffusion precipitin patterns. Quantitative studies of the saliva antigen levels by hemagglutination inhibition titration indicated a polygenic, quantitative mode of inheritance with a minimum heritability of O. 34. Blood group ABH secretor individuals were found …

The Molecular Nature And Replication Of R Factor 222 In Proteus Mirabilis, Dennis Jon Kopecko

Theses and Dissertations

The molecular nature and replicative behavior of R factor 222 was examined in Proteus mirabilis . In deoxyribonucleic acid (DNA} from R+ P. mirabilis , R factor 222 was identified by CsCl density gradient centrifugation as 2 satellite DNA bands at densities corresponding to 50 and 58 moles percent guanine plus cytosine (% GC) . Replication of the 50 and 58% GC components of R factor 222 in P. mirabilis was analyzed during growth in the presence and absence of chloramphenicol (CAM} and after shifting exponentialand stationary- phase cells to conditions which inhibit host protein or DNA synthesis . CAM …

Modulation Of Cortical And Pyramidal Tract Induced Motor Responses By Electrical Stimulation Of The Basal Ganglia, Roberta Ann Newton

Theses and Dissertations

Since the early 1800's, the basal ganglia have been recognized as an extrapyramidal subcortical motor area. Much of the knowledge of basal ganglia involvement in movement has been obtained through clinical symptomology. Consideration of the basal ganglia as a pure inhibitory influence upon motor regulation was a concept generated by early researchers (24,57,58). In spite of the fact that the basal ganglia was considered an inhibitory center, it was known that the basal ganglia produced fore limb flexion or contraversive head ‘turning. At the present, both facilitatory and inhibitory influences upon motor activity have been demonstrated (50). Physiological mechanisms underlying …

Mechanisms Of Drug Distribution In Rat Submaxillary Gland In Vitro, James W. Putney Jr.

Theses and Dissertations

The access of drugs to variofis sites of action in the body is impeded by a succession of membranes. Likewise, the removal of a drug or foreign substance from the body is similarly dependent on the ability of the substance under consideration to permeate biological barriers. A con-committant problem generally arises concerning the overall time course of drug action: that of drug storage through binding or similar processes. Schanker (196h) however, has stated that "Although the mechanisms of localization and those of membrane transfer are in many respects different problems, there are some instances in which they are inseparable parts …

Characterization Of Deoxyribonucleic Acid From Actinomycetes, Lynn William Enquist

Theses and Dissertations

Deoxyribonucleic acid (DNA) analyses were used to assess on a molecular level, the relationships among representatives of the genera Streptomyces, Nocardia and Mycobacterium. The methods developed in this study have been used for routine analysis of DNA from a large number of actinomycetes and have given reliable and reproducible data.

DNA isolated from various actinomycetes was characterized by buoyant density determinations in CsCl from which the mole fraction guanine plus cytosine (GC) content was calculated. All the streptomycete DNA preparations studied had buoyant densities in the range of 1.7287 to 1.7312 g cm^-3 which corresponded to GC compositions of …

A Variation Of The Standard Steepest Ascent Search Procedure For Response Surface Experiments, George Whitfield Cobb

Theses and Dissertations

The results of this chapter lead to a modification of the standard stopping rule which breathes new life into the method of steepest ascent, but which is unfortunately not sufficient to effect a total resuscitation. As expected, there is no uniformly optimum stopping rule, nor even a uniformly best test statistic; both the optimum statistic and the optimum test level depend on the model parameters y1 and y2. One can, however, adopt a compromise rule which works reasonably well for all parameter combinations by testing at the 25% level with the statistic zn = 1/2(yn -yn-2). In many cases the …

Glycogen Levels In Cardiac And Skeletal Muscle Of Rats Recovering From Exercise, Walter Talley Judd

Theses and Dissertations

In seeking answers to questions about how the human body functions under stressful situations, scientists, coaches, doctors, and athletes in general have all wondered about the role of exercise in the maintenance of good health. Good cardiovascular health, especially, is sought ardently, as it appears to be a major key to overall body fitness. Good cardiovascular health appears to go hand-in-hand with proper diet, proper rest, and proper exercise (1, 2).

The exercise phenomenon has attracted much attention and poses many questions. Just what is occurring when a person or an animal gets "in shape" by exercising. What bodily changes …

The Effects Of Alcohol On Three Levels Of Complex Human Behavior, Grady Vancil Maraman

Theses and Dissertations

That alcohol affects human behavior has been known for centuries. However, how and why it affects behavior has not been precisely established despite extensive research and study. The inconsistent action of alcohol at different doses on different tissue systems and on different response systems has been well documented (Kalant 1 referenced in 39). The exact sites of action of alcohol in the central nervous system and the relative influence of each of these sites in cognitive and motor behavior are not known. The primary purpose of this experiment was to study the effects of blood alcohol concentrations below 0.100 percent …

Evaluation Of The Essentiality Of Dextran In The Dental Caries Process, William Redman Grigsby

Theses and Dissertations

In summary, the main points to support the view that dextran synthesis is unessential to the dental caries process are presented below:

1 . Dextran-fed, microbe-bearing rats do not experience dental caries.

2 . Lactobacillus casei 4646 has no extracellular dextransucrase enzyme or other extracellular enzymes which release glucose or fructose from sucrose .

3. L. casei 4646 does not synthesize extracellular polysaccharide of the dextran class when grown on sucrose-containing medium.

4. L. casei 4646 does not exhibit in vitro, adherent growth on stainless-steel wire when grown on sucrose-containing medium .

5. L. casei 4646 does not cause dental …

Notebook 6, Newton H. Ancarrow

Ancarrow Wildflower Digital Archive Supplemental

Loose-leaf in binder.