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Full-Text Articles in Life Sciences
High-Throughput Rna Sequencing Of Pseudomonas-Infected Arabidopsis Reveals Hidden Transcriptome Complexity And Novel Splice Variants, Brian E. Howard, Qiwen Hu, Ahmet Can Babaoglu, Manan Chandra, Monica Borghi, Xiaoping Tan, Luyan He, Heike Winter-Sederoff, Walter Gassmann, Paola Veronese, Steffen Heber
High-Throughput Rna Sequencing Of Pseudomonas-Infected Arabidopsis Reveals Hidden Transcriptome Complexity And Novel Splice Variants, Brian E. Howard, Qiwen Hu, Ahmet Can Babaoglu, Manan Chandra, Monica Borghi, Xiaoping Tan, Luyan He, Heike Winter-Sederoff, Walter Gassmann, Paola Veronese, Steffen Heber
Biology Faculty Publications
We report the results of a genome-wide analysis of transcription in Arabidopsis thaliana after treatment with Pseudomonas syringae pathovar tomato. Our time course RNA-Seq experiment uses over 500 million read pairs to provide a detailed characterization of the response to infection in both susceptible and resistant hosts. The set of observed differentially expressed genes is consistent with previous studies, confirming and extending existing findings about genes likely to play an important role in the defense response to Pseudomonas syringae. The high coverage of the Arabidopsis transcriptome resulted in the discovery of a surprisingly large number of alternative splicing (AS) events …
Loss-Of-Function Of Constitutive Expresser Of Pathogenesis Related Genes5 Affects Potassium Homeostasis In Arabidopsis Thaliana, Monica Borghi, Ana Rus, David E. Salt
Loss-Of-Function Of Constitutive Expresser Of Pathogenesis Related Genes5 Affects Potassium Homeostasis In Arabidopsis Thaliana, Monica Borghi, Ana Rus, David E. Salt
Biology Faculty Publications
Here, we demonstrate that the reduction in leaf K+ observed in a mutant previously identified in an ionomic screen of fast neutron mutagenized Arabidopsis thaliana is caused by a loss-of-function allele of CPR5, which we name cpr5-3. This observation establishes low leaf K+ as a new phenotype for loss-of-function alleles of CPR5. We investigate the factors affecting this low leaf K+ in cpr5 using double mutants defective in salicylic acid (SA) and jasmonic acid (JA) signalling, and by gene expression analysis of various channels and transporters. Reciprocal grafting between cpr5 and Col-0 was used to determine the relative importance of …