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Articles 1 - 7 of 7

Full-Text Articles in Life Sciences

Widespread Triploidy In Western North American Aspen (Populus Tremuloides), Karen E. Mock, Colin M. Callahan, M. Nurul Islam-Faridi, John D. Shaw, Hardeep S. Rai, Stewart C. Sanderson, Carol A. Rowe, Ronald J. Rye, Michael D. Madritch, Richard S. Gardner, Paul G. Wolf Jan 2012

Widespread Triploidy In Western North American Aspen (Populus Tremuloides), Karen E. Mock, Colin M. Callahan, M. Nurul Islam-Faridi, John D. Shaw, Hardeep S. Rai, Stewart C. Sanderson, Carol A. Rowe, Ronald J. Rye, Michael D. Madritch, Richard S. Gardner, Paul G. Wolf

Biology Faculty Publications

We document high rates of triploidy in aspen (Populus tremuloides) across the western USA (up to 69% of genets), and ask whether the incidence of triploidy across the species range corresponds with latitude, glacial history (as has been documented in other species), climate, or regional variance in clone size. Using a combination of microsatellite genotyping, flow cytometry, and cytology, we demonstrate that triploidy is highest in unglaciated, drought-prone regions of North America, where the largest clone sizes have been reported for this species. While we cannot completely rule out a low incidence of undetected aneuploidy, tetraploidy or duplicated loci, our …


An Improved Competitive Inhibition Enzymatic Immunoassay Method For Tetrodotoxin Quantification, James P. Strange, Becky L. Williams, Susannah S. French Jan 2012

An Improved Competitive Inhibition Enzymatic Immunoassay Method For Tetrodotoxin Quantification, James P. Strange, Becky L. Williams, Susannah S. French

Biology Faculty Publications

Quantifying tetrodotoxin (TTX) has been a challenge in both ecological and medical research due to the cost, time and training required of most quantification techniques. Here we present a modified Competitive Inhibition Enzymatic Immunoassay for the quantification of TTX, and to aid researchers in the optimization of this technique for widespread use with a high degree of accuracy and repeatability.


Strong Self-Limitation Promotes The Persistence Ofrare Species, G. M. Yenni, P. B. Adler, S.K. Morgan Ernest Jan 2012

Strong Self-Limitation Promotes The Persistence Ofrare Species, G. M. Yenni, P. B. Adler, S.K. Morgan Ernest

Biology Faculty Publications

Theory has recognized a combination of niche and neutral processes each contributing, with varying importance, to species coexistence. However, long-term persistence of rare species has been difficult to produce in trait-based models of coexistence that incorporate stochastic dynamics, raising questions about how rare species persist despite such variability. Following recent evidence that rare species may experience significantly different population dynamics than dominant species, we use a plant community model to simulate the effect of disproportionately strong negative frequency dependence on the long-term persistence of the rare species in a simulated community. This strong self-limitation produces long persistence times for the …


Improved Ex Vivo Method For Microbiocidal Activity Across Vertebrate Species, Susannah S. French, Lorin A. Neuman-Lee Jan 2012

Improved Ex Vivo Method For Microbiocidal Activity Across Vertebrate Species, Susannah S. French, Lorin A. Neuman-Lee

Biology Faculty Publications

The field of ecoimmunology is currently undergoing rapid expansion, whereby biologists from a wide range of ecological disciplines are increasingly interested in assessing immunocompetence in their study organisms. One of the key challenges to researchers is determining what eco-immune measures to use in a given experiment. Moreover, there are limitations depending on study species, requirements for specific antibodies, and relevance of the methodology to the study organism. Here we introduce an improved ex vivo method for microbiocidal activity across vertebrate species. The utility of this assay is that it determines the ability of an organism to remove a pathogen that …


Methodological Tools, Ethan P. White, Xiao Xiao, J. B. Nick, Richard M. Sibly Jan 2012

Methodological Tools, Ethan P. White, Xiao Xiao, J. B. Nick, Richard M. Sibly

Biology Faculty Publications

Methodological approaches for comparing metabolic theory predictions to empirical data fall into two general categories: 1) determining if the general functional form of a relationship predicted by the theory is valid; and 2) determining whether the observed values of the parameters match the specific quantitative predictions made by the theory. Both of these categories of analysis rely on being able to accurately determine the best fitting form of a model with the same general functional form as that of MTE, so we will begin by discussing how this has typically been done using OLS regression on appropriately transformed data. Potential …


Investigating Human Impact In The Environment With Faded Scaffolded Inquiry Supported By Technologies, D. T. Longhurst, M. Longhurst, A. M. Duffy, Paul G. Wolf Jan 2012

Investigating Human Impact In The Environment With Faded Scaffolded Inquiry Supported By Technologies, D. T. Longhurst, M. Longhurst, A. M. Duffy, Paul G. Wolf

Biology Faculty Publications

Teaching science as inquiry is advocated in all national science education documents and by leading science and science teaching organizations. In addition to teaching science as inquiry, we recognize that learning experiences need to connect to students’ lives. This article details how we use a sequence of faded scaffolded inquiry supported by technologies to engage students meaningfully in science connected to their lives and schoolyards. In this approach, more teacher guidance is provided earlier in the inquiry experiences before this is faded later in the sequence, as students are better prepared to complete successful inquiries. The sequence of inquiry experiences …


Interfamily Variation In Amphibian Early Life-History Traits: Raw Material For Natural Selection?, Gareth R. Hopkins, Brian G. Gall, Susannah S. French, Edmund D. Brodie Jr. Jan 2012

Interfamily Variation In Amphibian Early Life-History Traits: Raw Material For Natural Selection?, Gareth R. Hopkins, Brian G. Gall, Susannah S. French, Edmund D. Brodie Jr.

Biology Faculty Publications

The embryonic development and time to hatching of eggs can be highly adaptive in some species, and thus under selective pressure. In this study, we examined the underlying interfamily variation in hatching timing and embryonic development in a population of an oviparous amphibian, the rough-skinned newt (Taricha granulosa). We found significant, high variability in degree of embryonic development and hatching timing among eggs from different females. Patterns of variation were present regardless of temperature.We also could not explain the differences among families by morphological traits of the females or their eggs. This study suggests that the variation necessary for natural …