Life Sciences Commons^™

Screening For Potential Therapeutic Targets Of Yb-1 Protein Using A Bioinformatics Approach, Omar Muneer Karkoutly

Theses and Dissertations

Treatment options for cancer are becoming much more limited due to the robust characteristics of cancer that allows them to rapidly develop drug resistance. This may be a result of cancer cells’ ability to switch between differentiated and undifferentiated states (plasticity). Diagnostic measurement and detection of cancer and its progression is essential for developing successful treatments. Specific cancer targets whose expressions are highly associated with increased incidence, risk, and spread of cancer therefore become perfect targets for therapeutic intervention. One such novel target that is still being studied is the Y-box binding protein 1 (YB-1), which …

Biomass Residues Improve Soil Chemical And Biological Properties Reestablishing Native Species In An Exposed Subsoil In Brazilian Cerrado, Thaís Soto Boni, Engil Isadora Pujol Pereira, Adriana Avelino Santos, Ana Maria Rodrigues Cassiolato, Kátia Luciene Maltoni

School of Earth, Environmental, & Marine Sciences Faculty Publications

Revegetation of exposed sub-soil, while a desirable strategy in the recovery processes, often fails due to extreme soil chemical properties, such as low organic matter and pH levels inhospitable to biological activities such as nutrients cycling and plant establishment. This is the case for approximately 800 ha of the Cerrado biome in Brazil, where erecting the embankment of a hydroelectric dam in the 1960’s stripped vegetation, soil, and subsoil layers thereby distorting the soil properties. This work evaluates the effectiveness of restoration management (RM) treatments, to restore the soil quality, including biological activity and chemical attributes. In a factorial scheme, …

A Participatory Assessment Of Nitrified Urine Fertilizer Use In Swayimane, South Africa: Crop Production Potential, Farmer Attitudes And Smallholder Challenges, Benjamin C. Wilde, Eva Lieberherr, Engil Isadora Pujol Pereira, Alfred Odindo, Johan Siz

School of Earth, Environmental, & Marine Sciences Faculty Publications

Long-term nutrient mining of soil hampers agricultural production across Africa. However, emerging sanitation technologies afford a hygienically safe and ecologically sustainable solution to this development challenge by providing fertilizers derived from human excreta that could facilitate a socio-technical transition toward a more sustainable food system. To evaluate one such technology, nitrified urine fertilizer (NUF), we conducted participatory action research to assess the potential, from both a biophysical and social perspective, of NUF to serve as a soil fertilizer to support smallholder agricultural production in Swayimane, South Africa. To achieve this objective, we formed a stakeholder group comprised of a cooperative …

Quantitative Relationships Between River And Channel-Belt Planform Patterns, Tian Y. Dong, Timothy A. Goudge

School of Earth, Environmental, & Marine Sciences Faculty Publications

Channel planform patterns arise from internal dynamics of sediment transport and fluid flow in rivers and are affected by external controls such as valley confinement. Understanding whether these channel patterns are preserved in the rock record has critical implications for our ability to constrain past environmental conditions. Rivers are preserved as channel belts, which are one of the most ubiquitous and accessible parts of the sedimentary record, yet the relationship between river and channel-belt planform patterns remains unquantified. We analyzed planform patterns of rivers and channel belts from 30 systems globally. Channel patterns were classified using a graph theory-based metric, …

The Estuarine Ecological Knowledge Network Makes Progress: International Project Sites And Potential Ways Forward, Grant S. Mccall, Anthony Akpan, Doru Banaduc, Diego F. Figueroa, Nelson Fontoura, Robert Hitchcock, Sherman Horn, Devashish Kar, Thong Tran

School of Earth, Environmental, & Marine Sciences Faculty Publications

The Estuarine Ecological Knowledge Network (EEKN) brings together scientists and coastal fishing communities in seeking new ways forward for Earth's major river deltas and estuaries, including the Mississippi (United States), Rio Grande (United States), Danube (Romania/Ukraine), Ganges (India/Bangladesh), Niger (Nigeria), and Mekong (Vietnam) river deltas, and the Patos Lagoon (Brazil). Such environments are universally understood as crucial for the biological productivity of oceans and they are home to hundreds of millions of human inhabitants, many of whom directly depend on that marine richness in terms of the operation of their socioeconomic systems. As human-induced climate change and its consequences for …

Differential Gene Expression In Tall Fescue Tissues In Response To Water Deficit, Manohar Chakrabarti, Padmaja Nagabhyru, Christopher L. Schardl, Randy D. Dinkins

School of Integrative Biological & Chemical Sciences Faculty Publications

Tall fescue (Festuca arundinacea Schreb.) is a popular pasture and turf grass particularly known for drought resistance, allowing for its persistence in locations that are unfavorable for other cool-season grasses. Also, its seed-borne fungal symbiont (endophyte) Epichloë coenophiala, which resides in the crown and pseudostem, can be a contributing factor in its drought tolerance. Because it contains the apical meristems, crown survival under drought stress is critical to plant survival as well as the endophyte. In this study, we subjected tall fescue plants with their endophyte to water-deficit stress or, as controls with normal watering, then compared plant …

Optimizing Stable Isotope Sampling Design In Terrestrial Movement Ecology Research, Andrea Contina, Sarah Magozzi, Hannah B. Vander Zanden, Gabriel J. Bowen, Michael B. Wunder

School of Integrative Biological & Chemical Sciences Faculty Publications

1. The recognition of adequate sampling designs is an interdisciplinary topic that has gained popularity over the last decades. In ecology, many research questions involve sampling across extensive and complex environmental gradients. This is the case for stable isotope analyses, which are widely used to characterize large-scale movement patterns and dietary preferences of organisms across taxa. Because natural-abundance stable isotope variation in the environment is incorporated into inert animal tissues, such as feathers or hair, it is possible to draw inferences about the type of food and water resources that individuals consumed and the locations where tissues were synthesized. However, modern …

Physical Activity Energy Expenditure Predicts Quality Of Life In Ambulatory School-Age Children With Cerebral Palsy, Jinuk Lee, Min-Hwa Suk, Soojin Yoo, Jeong-Yi Kwon

Health & Human Performance Faculty Publications

Background: Participation in physical activities is positively associated with better quality of life in children with cerebral palsy (CP). The objective of this study was to elucidate the relationship between the intensity of habitual physical activity (HPA) measured with an accelerometer and health-related quality of life (HRQOL) in school-age children with CP.

Method: A secondary analysis of the cross-sectional data of 46 ambulatory children with CP was conducted. The participants wore an accelerometer for seven days to measure HPA: activity counts (counts/min) and physical activity energy expenditure (PAEE, kcal/kg/day), as well as %moderate-to-vigorous intensity physical activity (%MVPA), %light intensity physical …

Where To Invest Project Efforts For Greater Benefit: A Framework Formanagement Performance Mapping With Examples For Potato Seed Health, C. E. Buddenhagen, Y. Xing, J. L. Andrade-Piedra, G. A. Forbes, P. Kromann, I. Navarrete, S. Thomas-Sharma, Robin A. Choudhury, K. F. Andersen Onofre, E. Schulte-Geldermann

School of Earth, Environmental, & Marine Sciences Faculty Publications

Policymakers and donors often need to identify the locations where technologies are most likely to have important effects, to increase the benefits from agricultural development or extension efforts. Higher-quality information may help to target the high-benefit locations, but often actions are needed with limited information. The value of information (VOI) in this context is formalized by evaluating the results of decision making guided by a set of specific information compared with the results of acting without considering that information. We present a framework for management performance mapping that includes evaluating the VOI for decision making about geographic priorities in regional …

Modeling The Impact Of Climate Change On Cervid Chronic Wasting Disease In Semi-Arid South Texas, Md Rafiul Islam, Ummugul Bulut, Teresa Patricia Feria-Arroyo, Michael G. Tyshenko, Tamer Oraby

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Chronic wasting disease (CWD) is a spongiform encephalopathy disease caused by the transmission of infectious prion agents. CWD is a fatal disease that affects wild and farmed cervids in North America with few cases reported overseas. Social interaction of cervids, feeding practices by wildlife keepers and climate effects on the environmental carrying capacity all can affect CWD transmission in deer. Wildlife deer game hunting is economically important to the semi-arid South Texas region and is affected by climate change. In this paper, we model and investigate the effect of climate change on the spread of CWD using typical climate scenarios. …

Stinging The Predators: A Collection Of Papers That Should Never Have Been Published, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

This ebook collects academic papers and conference abstracts that were meant to be so terrible that nobody in their right mind would publish them. All were submitted to journals and conferences to expose weak or non-existent peer review and other exploitative practices. Each paper has a brief introduction. Short essays round out the collection.

The Potential Impact Of Climate Change On The Distributions Of Eleutherodactylus Cystignathoides And E. Planirostris (Anura: Eleutherodactylidae), Rebecca T. Chastain

Theses and Dissertations

Climate change is inducing changes in the distributions of many species, causing range shifts and habitat loss as well as facilitating invasions. It is a broad contributor to global amphibian decline, already causing mass extinctions and extirpations of amphibian populations, a phenomenon which is expected to continue. The understudied direct-developing frog Eleutherodactylus cystignathoides is a notable outlier, having rapidly expanded its distribution in recent years. This is of particular interest given that other eleutherodactylids, such as E. coqui and E. planirostris, have already shown propensity for rapid dispersal and invasion. Originally found in the southernmost tip of …

Zinc-Mediated Regulatory Function Of Psd-95 On Nmdar At Post-Synapses, Luis F. Acosta

Theses and Dissertations

Postsynaptic density-95 (PSD-95) is a membrane-associated guanylate kinase that mediates localization of receptors in the excitatory postsynaptic density. It has been reported that PSD-95 mediates postsynaptic localization of NMDA receptors and anchors postsynaptic AMPAR receptors mainly through its postsynaptic membrane targeting by its N-terminal palmitoylation. Recent studies have shown that Ca2+/calmodulin blocks palmitoylation of PSD-95 by binding at the N-terminus of PSD-95, which promotes dissociation of PSD-95 from the postsynaptic membrane and causes loss of surface AMPARs in cultured neurons. Another metal ion zinc is found in various areas of the brain. As an endogenous neuromodulator, zinc plays a role …

Targeting Protein Synthesis In Clostridioides Difficile To Develop Antimicrobial Candidate, Elvira L. Alanis

Theses and Dissertations

Clostridioides difficile is a gram positive, spore forming, obligate anaerobic bacterium that causes Clostridioides difficile infection (CDI) which can result in pseudomembranous colitis and toxic megacolon. In the case of this research a focus on Protein synthesis is made since it is an essential metabolic process and is a validated target for antibiotics. Protein synthesis begins with Initiation which includes the three translation initiation factors. Through the NMR chemical shift assignments, the IF1 structure was determined, and it was found to be composed of a short α helix and five β helixes. A peptide was designed after the short α …

Harvester Ant (Pogonomyrmex Sp.) Seed Preferences And Distribution In A Suburban Setting, Lilly V. Elliott

Theses and Dissertations

Harvester ants (Pogonomyrmex sp.) are omnivorous ants native to Texas and are the main food source for the threatened Texas Horned Lizard (Phrynosoma cornutum). Little research has been conducted on harvester ants in the Lower Rio Grande Valley (LRGV) and their interactions with their environment. For this purpose, a variety of experiments were conducted to better understand these interactions and preferences. In CHAPTER I, suburban harvester ants were exposed commonly used cover crop seeds in the LRGV and inoculated seeds via seed depots over the course of 24 hours. We found that harvester ants do …

Effects Of Tributyltin Exposure On 8-Ohdg And Dsdna Expressions, Oxidative And Nitrative Stress In The American Oyster, Crassostrea Virginica, Mohan Kumar Dash

Theses and Dissertations

Environmental pollution increases due to anthropogenic activities. Different types of pollutants and/or chemicals impair growth, reproduction and development in aquatic organisms. Tributyltin (TBT, an organotin compound) is a tremendously toxic substance which widely uses as antifouling paints used in boats, hulls, and ships. The toxic effect of TBT is well documented in teleost species. The American oyster (Crassostrea virginica) is an ideal shellfish species to study on TBT exposure DNA lesion and oxidative/nitrative stress. In this study, the effects of TBT on 8’-hyrodxy-2’-deoxyguanosine (8-OHdG, a molecular marker), dsDNA, dinitrophenyl protein …

Postprandial Hemodynamics In Hispanics With And Without A Family History Of Type 2 Diabetes, Gabriel Alejandro Figueroa

Theses and Dissertations

Hispanics of the Rio Grande Valley (RGV) have the highest rates of obesity and type 2 diabetes (T2D) in the country, conditions often associated with cardiovascular disease and increased mortality. This study investigated the physiological factors affecting postprandial hemodynamic responses differently between FH+ and FH- groups. Thirty-one healthy Hispanic individuals volunteered in this study. Overall, FH+ individuals had higher pressures at rest compared to FH-, However, these differences were not statistically significant. When controlling for blood chemistries such as blood glucose, cholesterol, lipoproteins, and triglycerides, family history appears to have no effect on fasted or postprandial hemodynamics and pulse wave …

Nanostructured Materials For Biomedical Applications, Silverio Lopez

Theses and Dissertations

Nanotechnology has been used in an increasing number of applications in biochemistry and molecular biology. Compared to bulk materials, nanoscale materials offer a variety of advantages in applications due to an increased surface-to-volume ratio of the reactants. The unique properties of nanostructured materials and their properties such as stability, reactivity, magnetic response, pressure discharge performance and biocompatibility depend on particle size and shape. This work focuses on a nanoenergetic material composed of Aluminum (Al), Copper II oxide (CuO), and Iodine (I) pentoxide (I2O5), and the biomedical application of nanoscale dextran-coated magnetite (Fe3O4), which was produced using microfluidics to tune particle …

Exploring Dna Compaction Via Bacillus Subtilis Parb Protein By Single Molecule Investigation, Miranda L. Molina

Theses and Dissertations

Faithful chromosome segregation involves ParB, a DNA-binding and compacting protein that specifically recognizes parS DNA sites near the replication origin. ParB spreads 10-20 kb from parS sites, which recruitments additional ParB molecules to neighboring DNA, forming higher-order nucleoprotein complexes. ParB is also a novel CTPase enzyme and hydrolyses CTP to self-dimerization and create a clamp that slides along DNA. To understand the roles of CTP in Bacillus subtilis ParB (BsParB), we purified wild type ParB (BsParB (WT)) and recombinant ParB (BsParB(R80A)) mutant. BsParB(R80A) was known to prevent CTP binding and not compact DNA. Proteins N- and C-terminally appended with a …

Structures And Regulations Of Translation Initiation Factors In Pseudomonas Aeruginosa Protein Biosynthesis To Develop New Potential Antimicrobial Agents, Nicolette Valdez

Theses and Dissertations

Pseudomonas aeruginosa is a Gram-negative, multidrug resistant bacterium that has been recognized as the prominent cause of nosocomial infections. Untreated acute and chronic infections associated with P. aeruginosa can develop into other diseases such as pneumonia, otitis externa, and osteomyelitis. P. aeruginosa infections commonly affect immunocompromised individuals such as cystic fibrosis, cancer, and burn patients. The misuse and overuse of antibiotics has contributed to the increased antibiotic resistance in P. aeruginosa and thus has led to an unmet need for discovery of novel antibiotic candidates. Protein biosynthesis is an essential metabolic process occurring in all bacteria and a validated target …

Winners And Losers In Dryland Reforestation: Species Survival, Growth, And Recruitment Along A 33-Year Planting Chronosequence, Clifton Albrecht, Zarek Contreras, Kimberly Wahl-Villarreal, Mitch Sternberg, Bradley O. Christoffersen

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Reforestation in the Lower Rio Grande Valley (LRGV) of Texas began in the 1960s and to date over 6,475 ha of land has been reforested. However, there has been minimal assessment to understand differential species success, compositional trends, and the aboveground C sequestration potential of these developing forests. We coupled quantitative planting information of >50 native woody tree and shrub species with surveys of 5,223 stems of 4,606 individuals in a chronosequence of restored forests ranging in age from 1 to 33 years to estimate species-specific mortality rates, biomass accumulation and recruitment, as well as compositional trends in the herbaceous …

Removal Of Arsenic (Iii) And Arsenic (V) From Aqueous Solution Via Common Sunflower Helianthus Annuus By Phytoremediation, Irving A. Vazquez Hurtado

Theses and Dissertations

Phytoremediation provides a cost-effective, non-invasive technique to remove contaminants from the soil and groundwater. Sunflowers are fast growing and have a showed effective at removing various pollutants from soil, including lead and other heavy metals. Health issues related to arsenic are mainly attributed to exposure of arsenite, whereas arsenate is much less toxic. The common sunflower Helianthus annuus was the species used to remove both arsenic (III) and As(V) ions from hydroponics solutions. In the present study sunflower seeds were germinated for a week, placed under the sun for a day and subsequently placed in a nutrient solution to aid …

Assessing The Effects Of Levonorgestrel (Lng) On Neural Differentiation In The Developing Hypothalamus Using The Zebrafish (Danio Rerio) As A Model, Gabriella Hinojosa

Theses and Dissertations

Neurogenesis is a critical component of fetal neurodevelopment consisting of: migration, proliferation and differentiation. Health concerns from anxiety to reproductive issues have been linked to alterations of neurogenesis processes. Endocrine disrupting chemicals (EDCs) can mimic hormones vital to this process, potentially adversely affecting the susceptible fetal brain. One EDC, Levonorgestrel (LNG), is a testosterone-like progestin used in birth control methods that has a high bioavailability and long half-life. Using zebrafish embryos as a model, this lab previously discovered that an environmentally relevant LNG dose (5 ng/L) significantly increased anxiety behaviors. The hypothalamus is responsible for the development …

Comparing Various Physiological System Adaptations Following 8 Weeks Of Resistance Training With Or Without Bfr In Older Males, Ricardo Parra

Theses and Dissertations

PURPOSE: The purpose of this study was to compare the effects of 8 weeks of resistance exercise training with and without BFR on: 1), arterial elasticity and hemodynamics (e.g., blood pressure and resting heart rate), 2) muscular force production (e.g., maximal voluntary contraction, force production, electromyography, and estimated one-repetition maximum) in males between the 50 to 70 years of age.

RESULTS: Significant condition difference for reflection magnitude occurred in BFR group (p < 0.05). Significant time*condition effect occurred in Leg Press 1RM, Leg Extension 1RM, Leg Curl 1RM, Chest Press 1RM, and Shoulder Press 1RM (p < 0.05). Significant condition* time interaction occurred for Isokinetic Leg Extension Test (60°/s) (p < 0.05). A significant time*condition interaction occurred during Maximal Voluntary Contraction (MVC) – Root Mean Square for both vastus medialis and vastus lateralis (p < 0.05). A significant time main effect occurred in the BFR group for the amplitude mean of the MVC in the vastus medialis and vastus lateralis (p < 0.05). A significant time main effect occurred for median frequency of the MVC (p < 0.05). A significant difference in Estimated Visceral Adipose Tissue occurred in the BFR group (p < 0.05). A significant condition*time interaction occurred in arm fat percentage (p < 0.05). The BFR group had significantly less fat tissue (%) in the left arm (p < 0.01) and in the right arm (p = 0.04). A significant time*main effect occurred in the total lean tissue for the left side of the body (p = 0.04).

CONCLUSION: Using BFR resistance training at a low intensity can achieve similar adaptations seen at high intensities while reducing the risk of injury that comes with heavier loads.

Red Harvester Ant (Pogonomyrmex Barbatus F. Smith; Hymenoptera: Formicidae) Preference For Cover Crop Seeds In South Texas, Lilly Victoria Elliott-Vidaurri, Daniella Rivera, Adrian Noval, Robin A. Choudhury, Hannah J. Penn

School of Earth, Environmental, & Marine Sciences Faculty Publications

Harvester ants often selectively forage seeds, causing these ants to be viewed as pests in agricultural areas where they may forage on crop seeds. While little research has been done on harvester ant preferences for cover crop seeds, grower observations in the Lower Rio Grande Valley (LRGV) suggest that ants may remove these seeds before germination. We examined red harvester ant (Pogonomyrmex barbatus F. Smith) preferences for cover crop seeds (fescue, oat, sunn hemp, radish, vetch, and wheatgrass) and the effects of a commonly used bacterial seed inoculant. We evaluated relative preferences using seed depots presented to colonies with no …

Towards Conserving Crop Wild Relatives Along The Texas–Mexico Border: The Case Of Manihot Walkerae, Gisel Garza, Crystian Sadel Venegas Barrera, Jon Dale, José Guadalupe Martínez-Ávalos, Teresa Patricia Feria-Arroyo

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Walker’s Manihot, Manihot walkerae, is an endangered species endemic to south Texas and northeastern Mexico and is a Crop Wild Relative (CWR) of the international and economically important crop cassava (M. esculenta). Manihot walkerae is globally endangered (IUCN’s Redlist, Texas list, USA); however, it is not recognized on the Mexican list of endangered species (NOM-059-SEMARNAT). We assessed the status of M. walkerae in Mexico and re-evaluated its global status. According to our analysis, M. walkerae should be considered an endangered species based on the IUCN’s assessment method and a threatened species in Mexico based on the Mexican criteria. Our findings …

A Nematode Community-Based Integrated Productivity Efficiency (Ipe) Model That Identifies Sustainable Soil Health Outcomes: A Case Of Compost Application In Carrot Production, A. Habteweld, A. Kravchencko, Parwinder Grewal, H. Melakeberhan

School of Earth, Environmental, & Marine Sciences Faculty Publications

Percent soil organic matter (SOM), pH and crop yield are among the biophysicochemical process-driven soil health indicators (SHIs). However, identifying sustainable soil health conditions using these SHIs is limited due to the lack of Integrated Productivity Efficiency (IPE) models. We define IPE as a concept that identifies best-to-worst-case soil health outcomes by assessing the effect of agronomic practices on weighted abundance of functional guilds (WAFG) of beneficial soil organisms and SHIs simultaneously. Expressing WAFG of all beneficial nematodes (x-axis) and SHIs (y-axis) as a percent of untreated control and regression of x and y reveals four quadrants describing worst-to-best-case outcomes …

Pre-Growth Environmental Stresses Affect Foodborne Pathogens Response To Subsequent Chemical Treatments, Amandeep Singh, Veerachandra K. Yemmireddy

School of Earth, Environmental, & Marine Sciences Faculty Publications

Foodborne pathogens such as Salmonella, E. coli O157:H7, and Listeria monocytogenes are known to survive under different environmental stresses with an effect on their physiological properties. The purpose of this study was to determine the effect of different environmental stresses on the foodborne pathogens response to subsequent chemical treatments. Three types of pathogens Salmonella, E. coli O157:H7, and Listeria monocytogenes were subjected to different environmental stresses: (i) Desiccation (ii) high salt (iii) low pH, and (iv) temperatures (14, 23, and 37 °C) during their growth. The cells harvested at their early stationary growth phase were subsequently subjected to chlorine (100 …

Brain Charts For The Human Lifespan, R. A. I. Bethlehem, J. Seidlitz, S. R. White, J. W. Vogel, K. Anderson, C. Adamson, S. Adler, G. S. Alexopoulos, E. Anagnostou, John Blangero

School of Medicine Publications

Over the past few decades, neuroimaging has become a ubiquitous tool in basic research and clinical studies of the human brain. However, no reference standards currently exist to quantify individual differences in neuroimaging metrics over time, in contrast to growth charts for anthropometric traits such as height and weight1. Here we assemble an interactive open resource to benchmark brain morphology derived from any current or future sample of MRI data (http://www.brainchart.io/). With the goal of basing these reference charts on the largest and most inclusive dataset available, acknowledging limitations due to known biases of MRI studies …

Development And Validation Of An Edna Protocol For Monitoring Endemic Asian Spiny Frogs In The Himalayan Region Of Pakistan, Muhammad Saeed, Muhammad Rais, Ayesha Akram, Maggie R. Williams, Kenneth F. Kellner, Syed A. Hashsham, Drew R. Davis

School of Earth, Environmental, & Marine Sciences Faculty Publications

Wildlife monitoring programs are instrumental for the assessment of species, habitat status, and for the management of factors affecting them. This is particularly important for species found in freshwater ecosystems, such as amphibians, as they have higher estimated extinction rates than terrestrial species. We developed and validated two species-specific environmental DNA (eDNA) protocols and applied them in the field to detect the Hazara Torrent Frog (Allopaa hazarensis) and Murree Hills Frog (Nanorana vicina). Additionally, we compared eDNA surveys with visual encounter surveys and estimated site occupancy. eDNA surveys resulted in higher occurrence probabilities for both A. …