Life Sciences Commons^™

Hairy Stalagmites, A New Biogenic Root Speleothem From Botswana, Gerhard C. Du Preez, Paolo Forti, Gerhard Jacobs, Anine Jordaan, Louwrens Tiedt

International Journal of Speleology

Ngamiland in northwestern Botswana hosts the Gcwihaba Caves which present unique subterranean environments and host speleothems never before recorded. Cave atmospheric conditions can be extreme with temperatures as high as 28°C and relative humidity nearing 99.9%. Within Dimapo and Diviner’s Caves peculiar root speleothems that we named ‘Hairy Stalagmites’ were found. These stalagmites are closely associated with the roots of Namaqua fig (Ficus cordata) trees that enter the cave environment in search of water. Pieces of broken stalagmites were sampled from Dimapo Cave for further investigations. Stereo and electron microscopy revealed that the Hairy Stalagmites consist of multiple …

Variability In The Spatial And Temporal Patterns Of Larval Scombrid Abundance In The Gulf Of Mexico, Sennai Y. Habtes

USF Tampa Graduate Theses and Dissertations

Accurate fishery-independent methods for assessing the abundance of marine fish stocks are important tools for proper pelagic fisheries management. The review and improvement of standardized sampling methods used in fisheries-independent sampling, and the establishment of improved models of abundance and population dynamics utilizing novel statistical techniques for fisheries management will further improve our understanding of the way in which marine fish stocks vary spatially and temporally. One of the most important and longest running surveys of fisheries-independent data in the Southeastern United States is the spring ichthyoplankton survey conducted through the Southeast Area Mapping and Assessment Program (SEAMAP).

These surveys …

Reef-Scale Thermal Stress Monitoring Of Coral Ecosystems: New 5-Km Global Products From Noaa Coral Reef Watch, Gang Liu, Scott F. Heron, C. Mark Eakin, Frank E. Muller-Karger, María Vega-Rodriguez, Liane S. Guild, Jacqueline L. De La Cour, Erick F. Geiger, William J. Skirving, Timothy F. R. Burgess, Alan E. Strong, Andy Harris, Eileen Maturi, Alexander Ignatov, John Sapper, Jianke Li, Susan Lynds

Marine Science Faculty Publications

The U.S. National Oceanic and Atmospheric Administration (NOAA) Coral Reef Watch (CRW) program has developed a daily global 5-km product suite based on satellite observations to monitor thermal stress on coral reefs. These products fulfill requests from coral reef managers and researchers for higher resolution products by taking advantage of new satellites, sensors and algorithms. Improvements of the 5-km products over CRW’s heritage global 50-km products are derived from: (1) the higher resolution and greater data density of NOAA’s next-generation operational daily global 5-km geo-polar blended sea surface temperature (SST) analysis; and (2) implementation of a new SST climatology derived …

A Novel Role For Nf-Κb In Proximal T Cell Signaling, Crystina Bronk Watson

USF Tampa Graduate Theses and Dissertations

The interrogation of T cell signaling over the past fifty years has led to the discovery of amazingly intricate cascade networks and elaborate descriptions of individual proteins' domains and functions. A complex landscape has been rendered in which proteins relay messages from the extracellular ligation of the TCR by a cognate peptide loaded MHC via changes in sub-cellular location, phosphorylation, and binding affinities and partners to enact nuclear localization of three key transcription factors required for cellular effector function and proliferation: AP-1, NF-AT, and NF-κB. Dogma has favored activation of each of these transcription regulating elements to be a linear …

A Functional Approach To Resolving The Biogeocomplexity Of Two Extreme Environments, Haydn Rubelmann Iii

USF Tampa Graduate Theses and Dissertations

The biodiversity of two distinct marine environments was observed to describe the biogeocomplexity of these extreme ecological systems. A shallow-water hydrothermal vent in Papua New Guinea served as a study of a thermophilic ecosystem influenced by arsenic rich vent fluids while a 60 m deep offshore primarily anoxic karst sink served as a study of an anaerobic sulfur-influenced habitat. Both environments support unique biological communities that are influenced by the physical and chemical pressures imposed on them by the harsh conditions of these systems. In Tutum Bay, Ambitle Isle, Papua New Guinea, a transect was created from a shallow hydrothermal …

Investigations Of The Physical And Analytical Chemistry Of Iron In Aqueous Solutions, James Patten

USF Tampa Graduate Theses and Dissertations

Although iron occurs at extremely low concentrations in the world’s oceans, it is essential for all living organisms. It is the limiting nutrient in High Nutrient Low Chlorophyll (HNLC) areas of the ocean, and exerts critically important influences on levels of atmospheric CO₂ and the global carbon cycle. Understanding the chemical processes that govern the fluxes and biogeochemistry of oceanic iron requires thorough assessment of the aqueous physical chemistry of iron and analytical techniques capable of measuring iron at sub-nanomolar concentration measurements. This dissertation extends prior work on the physical and analytical chemistry of iron through (a) investigation of …

Functional Morphology And Feeding Mechanics Of Billfishes, María Laura Habegger

USF Tampa Graduate Theses and Dissertations

Billfishes (marlins, spearfishes, sailfishes and swordfish) are one of the fastest and largest marine apex predators, and perhaps their most recognizable attribute is their bill or rostrum. The proposed function for this novel structure has ranged from hydrodynamic enhancement to defensive weaponry. However, the most supported hypothesis for its function has been linked to feeding. Billfishes have been observed to subdue their prey with their rostrum, either stunning or cutting them into pieces before ingestion. Due to their large body sizes and pelagic lifestyles a thorough investigation of the function of this structure has been logistically challenging. The goal of …

Influences Of Yard Management Intensity On Urban Soil Biogeochemistry, Viviana Penuela Useche

USF Tampa Graduate Theses and Dissertations

Soils are critical to ecosystem function as they provide essential nutrients for primary producers, habitat and organic energy for decomposers, and storage of organic matter. Irrigation with reclaimed water is an increasingly popular water conservation strategy; yet its high salinity and nutrient content potentially affect soil properties. In this study, set in a residential neighborhood of Tampa (U.S.). I tested whether there are distinct lawn system management strategies characterized by systematic differences in reclaimed water usage and irrigation and fertilization practices. I then investigated whether soil biogeochemistry responds to lawn system management strategy.

My results indicated that amendment strategy, which …

Growth Rates In Gulf Of Mexico Red Snapper, Lutjanus Campechanus, Before And After The Deepwater Horizon Blowout, Elizabeth Shea Herdter

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout occurred on April 20^th, 2010 and released nearly 5 million barrels of crude oil into the northern Gulf of Mexico causing pollution of the water and sediment inhabited by many fishes for at least 87 days while the wellhead went uncapped. Populations of the Gulf of Mexico Red snapper, Lutjanus campechanus, an important fish to the ecology and economy in the region, exhibit affinity to shallow water oil infrastructure such as the Deepwater Horizon making them especially vulnerable to crude oil contamination. The objective of this study is to determine growth of Red …

Polycyclic Aromatic Hydrocarbon Metabolites As A Biomarker Of Exposure To Oil In Demersal Fishes Following The Deepwater Horizon Blowout, Susan Susan Snyder

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout occurred on April 20th, 2010, releasing 4.9 million barrels of Louisiana crude oil into the Gulf of Mexico (GoM). Subsequent to the Deepwater Horizon blowout, sediment cores revealed oil on the northern GoM seafloor and abnormal skin lesions were seen in GoM fishes. Exposure to polycyclic aromatic hydrocarbons (PAHs), a component of crude oil, in fish has been associated with many sublethal effects, including cancer and population-level effects. Using a biomarker of exposure to PAHs, this thesis evaluates inter-species, temporal and spatial differences in exposure to hydrocarbon contamination between three species of fish with varying levels …

Use Of A Towed Camera System For Estimating Reef Fish Populations Densities On The West Florida Shelf, Sarah Elizabeth Grasty

USF Tampa Graduate Theses and Dissertations

Reef fish species tend to reside over high relief habitat which makes them difficult to sample with traditional gears such as nets and trawls. Therefore, implementing and understanding the strengths and weaknesses of new approaches which incorporate acoustic and optical methods has become a priority for reef fish stock assessment. Beginning in June of 2013, a towed camera system known as the Camera-Based Assessment Survey System (C-BASS) has been used to visualize over 500 kilometers of transect and record more than 80 hours of video over several habitats in the Gulf of Mexico. Surveys have been completed on the West …

Cloning And Characterization Of Il-1Β, Il-8, Il-10, And Tnfα From Golden Tilefish (Lopholatilus Chamaeleonticeps) And Red Snapper (Lutjanus Campechanus), Kristina L. Deak

USF Tampa Graduate Theses and Dissertations

Cytokines are pleiotropic and redundant signaling molecules that govern the inflammatory response and immunity, a critical ecological parameter for organism success and population growth. Produced at the site of injury or pathogen intrusion by a variety of cell types, cytokines mediate cell-signaling in either an autocrine or paracrine manner. The type and magnitude of the cytokine milieu produced subsequently dictates the strength and form of immune response. As the most diverse vertebrate group, with a high sensitivity to contaminants, fish represent an important foci for the evaluation of immune system evolution, function, and alteration upon toxicant exposure. While many cytokines …

Dynamics And Survival Of Coral And Octocoral Juveniles Following Disturbance On Patch Reefs Of The Florida Reef Tract, Lucy Bartlett

USF Tampa Graduate Theses and Dissertations

Over the past several decades, rapid decline in adult stony-coral (comprising the Orders Scleractinia and Anthomedusae, specifically Family Milleporidae) cover has occurred concurrent with an increase in adult octocoral (Octocorallia/gorgonian) cover along the Florida Reef Tract. In January 2010, the Florida Keys experienced extremely cold air and water temperatures, below the lethal threshold for many reef organisms including corals. Very high stony-coral mortality occurred on some patch reefs. The newly-available space created by this disturbance event provided the opportunity for recruitment and settlement of new coral larvae and other reef organisms.

The goal of this study was to examine post-disturbance …

Assessments Of Surface-Pelagic Drift Communities And Behavior Of Early Juvenile Sea Turtles In The Northern Gulf Of Mexico, Robert F. Hardy

USF Tampa Graduate Theses and Dissertations

Knowledge of species distribution and habitat associations are essential for conservation measures. Such information is lacking for many marine species due to their occupancy of broad and ephemeral habitats that are difficult to access for study. Sea turtles, specifically the surface-pelagic juvenile stage of some species, are a group for which significant knowledge gaps remain surrounding their distribution and habitat use. Recent research has confirmed the long-standing hypothesis that the surface-pelagic juvenile stage occurs within surface-pelagic drift communities (SPDC). Within the North Atlantic and surrounding basins, the holopelagic macroalgae Sargassum spp. dominates SPDC and serves as a remotely-detectable indicator of …

Novel Therapeutic Strategies In Lung Cancer, Courtney A. Kurtyka

USF Tampa Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer-related death and the second most diagnosed cancer in the United States. Unfortunately, many patients either do not have any common mutations for which there are already targetable agents, or they eventually become resistant to these compounds. As such, there is a high demand for new, effective methods of treating this disease as well as predicting patient prognosis and potential benefit from chemotherapy. In this work, numerous strategies for treating lung cancer are explored.

The first method described here is through the use of a pan-early 2 factor (E2F) inhibitor, HLM006474, which is …

Un Cuento Satírico En Medio Del Debate Sobre El Darwinismo En México, Miguel A. Fernández Delgado Mafd

Alambique. Revista académica de ciencia ficción y fantasía / Jornal acadêmico de ficção científica e fantasía

Charles Darwin's theory of biological evolution of species was accepted or rejected by Mexican scientists, including Gabino Barreda, representative of Comte's philosophy. It was also included by Justo Sierra in a history book for the Escuela Nacional Preparatoria, a decision which raised a lot of criticism from conservative groups. It is also discussed the implications of social Darwinism in the early Twentieth Century Mexico. The document we offer is a satire published in those years, which resembles the tone of Swift's Gulliver Travels.

Stable Isotopes In Fish Eye Lenses As Potential Recorders Of Trophic And Geographic History, Amy A. Wallace, David J. Hollander, Ernst B. Peebles

Marine Science Faculty Publications

We evaluated eye lenses as potential recorders of stable isotope histories in fish because they consist of metabolically inert optical proteins that are deposited in successive, concentric circles (laminae) much like otolith circuli and tree rings. We conducted four different tests on lenses from red snapper, red grouper, gag, and white grunt. The first test was a low-resolution screening of multiple individuals (4–5 radial groups of laminae per lens, all species except white grunt). Along the radial axis, all individuals exhibited substantial isotopic variability. Red snapper individuals separated into two groups based on δ¹⁵N and gag separated into …

Effect Of High Pressure On Hydrocarbon-Degrading Bacteria, Martina Schedler, Robert Hiessl, Ana Gabriela Valladares Juárez, Giselher Gust, Rudolf Müller

C-IMAGE Publications

The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oil-degrading bacteria has been scarce. To investigate how the biodegradation of crude oil and its components is influenced by high pressures, like those in deep-sea environments, hydrocarbon degradation and growth of two model strains were studied in high-pressure reactors. The alkane-degrading strain Rhodococcus qingshengii TUHH-12 grew well on n-hexadecane at 15 MPa at a rate of 0.16 h(-1), although slightly slower …

Mechanisms And Molecular Biology Of Major Tumor Suppressors, Brienne E. Engel

USF Tampa Graduate Theses and Dissertations

This dissertation is devoted to the study of the molecular biology of major tumor suppressors, defined as those that prevent the cellular processes identified as the hallmarks of cancer. Specifically, the major tumor suppressors pRb and STK11 are explored in the context of osteosarcoma and lung cancer, respectively.

RB1 was the first tumor suppressor gene discovered. Over four decades of work have revealed that the Rb protein (pRb) is a master regulator of biological pathways influencing virtually every aspect of intrinsic cell fate including cell growth, cell-cycle checkpoints, differentiation, senescence, self-renewal, replication, genomic stability and apoptosis. While these many processes …

Role And Regulation Of Snon/Skil And Plscr1 Located At 3q26.2 And 3q23, Respectively, In Ovarian Cancer Pathophysiology, Madhav Karthik Kodigepalli

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is one of the most common causes of gynecological cancer related deaths in women. In 2014, the estimated number of deaths due to ovarian cancer is 14,270 with occurrence of over 22, 240 new cases (National Cancer Institute, http://seer.cancer.gov/statfacts/html/ovary.html). Despite improvement in treatment strategies, the 5-year survival rate is still below 50% mainly due to chemoresistance and relapse. Amplification of chromosomal region 3q26 is a common characteristic in various epithelial cancers including ovarian cancer. This region harbors various oncogenes including the TGFβ signaling mediators EVI1 and SnoN/SkiL, PKCι and PIK3CA amplified at 3q26.2 and 3q26.3, respectively, in ovarian …

Eukaryotic Gene Expression Patterns Of Microorganisms In The Amazon River Plume Parallel The Biogeochemistry Of Plume Waters, Brian L. Zielinski

USF Tampa Graduate Theses and Dissertations

Unraveling the microbiological processes that occur as water travels from a river's mouth into the ocean is critical to understanding the role of river plumes in global biogeochemical cycles. Metranscriptomics, the gene expression of a whole community of organisms, was utilized to examine six stations along the Amazon River Plume (ARP) in 2010 to test thehypothesis that there were measurable differences in gene expression for key biogeochemical genes along the ARP. This body of work focuses on methods developed to identify which genes are biogeochemically important for a particular environment along extreme salinity, nutrient and community gradients in the ARP, …

Assessing Climate Variability Effects On Dengue Incidence In San Juan, Puerto Rico, Pablo Méndez-Lázaro, Frank E. Muller-Karger, Daniel Otis, Matthew J Mccarthy, Marisol Peña-Orellana

Marine Science Faculty Publications

We test the hypothesis that climate and environmental conditions are becoming favorable for dengue transmission in San Juan, Puerto Rico. Sea Level Pressure (SLP), Mean Sea Level (MSL), Wind, Sea Surface Temperature (SST), Air Surface Temperature (AST), Rainfall, and confirmed dengue cases were analyzed. We evaluated the dengue incidence and environmental data with Principal Component Analysis, Pearson correlation coefficient, Mann-Kendall trend test and logistic regressions. Results indicated that dry days are increasing and wet days are decreasing. MSL is increasing, posing higher risk of dengue as the perimeter of the San Juan Bay estuary expands and shorelines move inland. Warming …

A Rapid Response Study Of The Hercules Gas Well Blowout, Samantha Joye, Joseph P. Montoya, Steven Murawski, Tamay Özgökmen, Terry L. Wade, Raffaele Montuoro, Brian J. Roberts, David Hollander, Wade H Jeffrey, Jeffrey Chanton

C-IMAGE Publications

On 20 April 2010, the Deepwater Horizon drilling rig lost well control while drilling at the Macondo prospect in the Gulf of Mexico. At the time of the Macondo blowout, the academic scientific community was ill prepared to initiate and rapidly conduct the necessary coordinated interdisciplinary studies of the environments around the discharge area.

Diatom Flora In Subterranean Ecosystems: A Review, Elisa Falasco, Luc Ector, Marco Isaia, Carlos E. Wetzel, Lucien Hoffmann, Francesca Bona

International Journal of Speleology

In scarcity of light and primary producers, subterranean ecosystems are generally extremely oligotrophic habitats, receiving poor supplies of degradable organic matter from the surface. Human direct impacts on cave ecosystems mainly derive from intensive tourism and recreational caving, causing important alterations to the whole subterranean environment. In particular, artificial lighting systems in show caves support the growth of autotrophic organisms (the so-called lampenflora), mainly composed of cyanobacteria, diatoms, chlorophytes, mosses and ferns producing exocellular polymeric substances (EPSs) made of polysaccharides, proteins, lipids and nucleic acids. This anionic EPSs matrix mediates to the intercellular communications and participates to the chemical …

Niche Differentiation In Meta Bourneti And M. Menardi (Araneae, Tetragnathidae) With Notes On The Life History, Stefano Mammola, Marco Isaia

International Journal of Speleology

Meta menardi and M. bourneti are two species of spiders inhabiting caves and other subterranean habitats. The occurrence of both species within the same cave has never been proved convincingly and several authors hypothesized a complete niche differentiation mainly based on microclimatic conditions.In order to study the apparent niche differentiation of the two species, we studied several populations of M. menardi and M. bourneti occurring in six caves in the Western Italian Alps (NW Italy). A series of squared plots were monitored monthly from March 2012 to February 2013. At each survey, we counted individuals and we collected the main …

Karst Estuaries: A Newly Described Ecosystem Governed By Aquifer Hydrology, Damian M. Menning

USF Tampa Graduate Theses and Dissertations

The overall goal of this dissertation is to define the hydrological, geochemical, and biological characteristics of a Karst Estuary. These types of estuaries represent a unique ecosystem created by freshwater inputs from direct flow through karst conduits and/or diffuse flow through a karst matrix. In order to determine the characteristics of a Karst Estuary we monitored short-term tidal fluctuations, long-term rainfall patterns, aquifer levels, spring discharge, multiple geochemical parameters, microbial communities in the water column and sediment, and macrofaunal communities in the sediment along a transect from a submarine spring through the Gulf of Mexico. Four sites were selected along …

A Record Of Anthropogenic Effects On Sedimentation In The Manatee River, Florida, Patrick Schwing, Ashanti Johnson

Marine Science Faculty Publications

Increased sedimentation rates have been attributed to increased anthropogenic activity in watersheds throughout Florida and many parts of the world. The Manatee River, located on the west coast of Florida (USA), like many other coastal watersheds, has experienced depletion in natural resources, increased nutrient loading, and increased pollution. LARs (linear accumulation rates) from watersheds throughout Florida suggest that anthropogenic activity increased bulk sedimentation by as much as 4-fold. The objective of this study was to construct a record of sedimentation and improve upon previous studies by determining individual sedimentary constituent MARs (mass accumulation rates) based on short lived radioisotopes (210Pb …

Silica Cycling In The Ultra-Oligotrophic Eastern Mediterranean Sea, M. D. Krom, N. Kress, Kent A. Fanning Ph.D.

Marine Science Faculty Publications

Although silica is a key plant nutrient, there have been few studies aimed at understanding the Si cycle in the eastern Mediterranean Sea (EMS). Here we use a combination of new measurements and literature values to explain the silicic acid distribution across the basin and to calculate a silica budget to identify the key controlling processes. The surface water concentration of ∼1 μM, which is unchanging seasonally across the basin, was due to the inflow of western Mediterranean Sea (WMS) water at the Straits of Sicily. It does not change seasonally because there is only a sparse population of diatoms …