Life Sciences Commons^™

A Comparison Of Imputation Algorithms For Modeling Water Quality, Carter Alexander Allen

Senior Theses

This project addresses the need for predictive modeling tools to forecast expected concentrations of fecal bacteria in recreational waters in the Charleston, SC area. Data was provided by Charleston Waterkeeper, a water quality monitoring organization that has been measuring Enterococcus faecalis concentrations at 15 recreational sites since 2013. The data contain a non-negligible number of censored and missing observations, so three distinct imputation methods were developed and compared in terms of their effect on final predictive model characteristics. The best performing method relied on drawing samples from a truncated normal distribution to replace censored values, and using a partial model …

Assessing Variation In Visual Abilities In Daphnia As A Result Of Disparity In Eye Sizes, Adrian Perez

Senior Theses

The functionality of an eye is affected by a number of structural factors, and altering any of these factors often involves inevitable tradeoffs between resolution and sensitivity. However, it has been theoretically reasoned, and tested empirically, that increasing the size of an eye allows for both of these central features of vision to be improved due to corresponding increases in lens size. This study examines the relevance of this hypothesis for the vision of Daphnia in both interspecific and intraspecific contexts. The visual capabilities of six different species of Daphnia are tested using the optomotor response—a visually mediated behavior that …

Potential For Aquaculture Integration With Wastewater Treatment Wetlands In Southern Iraq, Melaina Dyck

Senior Theses

Wetlands provide key ecosystem services that sustain human communities. The Mesopotamiam Marshes of southern Iraq continuously supported civilization there for 6000 years. A combination of upstream damming and intentional draining by the Iraqi government led the entire ecosystem to collapse, devastating local human communities. One of the most significant ecosystem services lost was water remediation, filtration and storage. Following the collapse of the marshes, water quality in southern Iraq declined to the point of being deleterious to human health and barely useful for farming or fish production. Wastewater treatment wetlands are a potential solution for restoring wetlands and remediating polluted …

An Illustrated Investigation Of Agraulis Vanillae And Its Larval Food Plants, Christiana M. Keinath

Senior Theses

To create my work, I directly observed caterpillars, chrysalises, and adult butterflies. I used a variety of media to depict salient aspects of gulf fritillary anatomy and to follow the species through its life stages in connection with its passionflower hosts. I focused on accurately recreating what I observed, as well as breaking down the organisms into their component colors, lines, shapes, and values. In my work I emphasized natural textures, which vary greatly as the caterpillars undergo their morphological changes. As a result of this project, I improved my observation and life drawing skills and learned how the process …

Tree Damage From Hurricane Matthew Affecting Forest Dynamics In Congaree National Forest, Connor Lentz

Senior Theses

The Congaree National Forest serves as an ecological model for bottomland-hardwood forests. Hurricanes periodically strike the Southeast United States, with high-wind speeds causing damage affecting the Congaree National Forest and modifying forest dynamics. This has been well-studied from the effects of Hurricane Hugo in September 1989 (Sharitz et al. 1993). In October 2016, Hurricane Matthew, a category 5 storm, made landfall in the Southeastern United States, and eventually moved through the Congaree National Forest. The high winds and precipitation caused widespread tree damage throughout Congaree. This study used a belt-transect survey of 14.7km of trails throughout the park to record …

Climate Change As A Contributor To Terrorism: A Case Study In Nigeria And Pakistan, Natalie Lytle

Senior Theses

This thesis explores the relationship between climate change and terrorism. It seeks to identify climate change as a contributing factor to terrorism. The motivation for this research is the increasing threat from both global issues. The threat of climate change and terrorism were heavily debated topics in the United States 2016 election, and remain contentious topics for world leaders today. The years 2014 and 2015 were the top two deadliest years for global terrorism, and out of the 17 hottest years on record, 16 of them have occurred since 2000. The world has already begun to see the repercussions of …

Causes And Consequences Of Individual Phenotypic Differences In Brachyuran Crabs With A Focus On Behavior, Benjamin A. Belgrad

Theses and Dissertations

Individual variation defines almost every morphological, physiological, and behavioral aspect of populations and is a fundamental component of many ecosystem processes. Recent work indicates that accounting for these individual differences can enhance our ability to predict community responses to environmental disturbances which is becoming increasingly important in an era of extraordinary global change. However, our understanding of how different individual characteristics are connected to each other and governed by the environment remains limited.

This study sought to evaluate the relationship between individual behavior, physiological condition, and local habitat for Brachyuran crabs as well as the subsequent strength of their predator-prey …

Tgf-Beta Triggers Rapid Fibrillogenesis Via A Novel T Beta Rii-Dependent Fibronectin-Trafficking Mechanism, Archana Varadaraj, Laura M. Jenkins, Priyanka Singh, Anindya Chanda, John Snider, N.Y. Lee, Ayelet R. Amsalem-Zafran, Marcelo Ehrlich, Yoav I. Henis, Karthikeyan Mythreye

Faculty Publications

Fibronectin (FN) is a critical regulator of extracellular matrix (ECM) remodeling through its availability and stepwise polymerization for fibrillogenesis. Availability of FN is regulated by its synthesis and turnover, and fibrillogenesis is a multistep, integrin-dependent process essential for cell migration, proliferation, and tissue function. Transforming growth factor β (TGF-β) is an established regulator of ECM remodeling via transcriptional control of ECM proteins. Here we show that TGF-β, through increased FN trafficking in a transcription- and SMAD-independent manner, is a direct and rapid inducer of the fibrillogenesis required for TGF-β–induced cell migration. Whereas TGF-β signaling is dispensable for rapid fibrillogenesis, stable …

Assessing Women's Attitudes Towards Genetic Testing For Hereditary Breast Cancer, Taylor Jane Apostolico

Theses and Dissertations

OBJECTIVES: Hereditary Breast and Ovarian Cancer (HBOC) is an autosomal dominant cancer predisposition syndrome with a 46-87% lifetime risk of breast cancer. Unaffected women who have HBOC are eligible for more screening procedures and prophylactic surgeries that may reduce the risk of developing cancer by up to 95%. The objectives of this study were to assess women’s awareness of and interest in breast cancer genetic testing services, as well as women’s attitudes and beliefs regarding the clinical utilization of HBOC genetic testing across demographic categories. METHODS: Two-hundred and sixty-eight women completed a 35-item survey designed to capture perceptions of HBOC …

The Energetic Consequences Of Temperature Variation And Sequential Autotomization For The Stone Crab, Menippe Spp., Eric R. Hancock

Theses and Dissertations

The stone crab, Menippe spp., is harvested in a claw only fishery along the Gulf and southeastern Atlantic coasts of the United States. As climate change continues to warm these areas, crabs are forced to cope with higher water temperatures and lower dissolved O2 concentrations. These altered environmental conditions may influence crab energetics by altering both energy intake and expenditure. To inform fishery sustainability as well as prepare for a potential range expansion of the crab, this study investigates the energetic intake and expenditure of individual stone crabs. Crabs were found to respire 80 and 69% more following the loss …

The Effects Of Low-Dose Ionizing Radiation On Voles From The Chernobyl Exclusion Zone, Justin Sindoni

Senior Theses

Much is known about the effects of high doses of ionizing radiation exposure, but little is known about the effects of low doses of ionizing radiation over extended periods of time. Nuclear power plant disasters, in particular the 1986 Chernobyl disaster, have led to long-term radioactive contamination of their surrounding areas, and more research is needed to learn about the effects of the contamination. In order to better understand the impacts of low-dose ionizing radiation on animal populations, bank voles (Myodes glareolus) from the Chernobyl exclusion zone were studied. Voles were captured from 64 sites throughout the exclusion zone, and …

Emerging Antimicrobial Research Against Superbugs: Perspectives From A Polymer Laboratory, Mitra S. Ganewatta, Md Anisur Rahman, Chuanbing Tang

Journal of the South Carolina Academy of Science

Infectious diseases caused by drug-resistant microorganisms have become a major contributor for human morbidity and mortality. To overcome such threats, we have developed various antimicrobial agents using natural product derivatives and metallopolymers. Abundant biomass such as resin acids can be utilized to prepare cationic polymers for inhibiting a variety of bacteria. These polymers have been used in solution as well as surfaces as antimicrobial materials with low cytotoxicity. In addition, a class of charged metallopolymers have been developed to kill superbugs such as MRSA.

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Antitumor Activity Of Selected Derivatives Of Pyrazole- Benzenesulfonamides From Dilithiated C(Α), N-Phenylhydrazones And Lithiated Methyl 2-(Aminosulfonyl)Benzoate, N. Dwight Camper [Deceased], James M. Gum, Darby E. Lyles, William T. Pennington, Charles F. Beam, Clyde R. Metz

Journal of the South Carolina Academy of Science

Several pyrazole-benzenesulfonamides were subjected to biological evaluation involving tumor formation on potato discs caused by Agrobacterium tumefaciens. This assay led to some excellent and promising initial results with three of the pyrazole compounds showing increased tumor inhibition when compared to a recognized standard, camptothecin. The select pyrazole-benzenesulfonamides were prepared by condensation-cyclization of several dilithiated C(α),N-phenylhydrazones with lithiated methyl 2-aminosulfonyl-benzoate.

Differential Response Of Coral Symbiotic Dinoflagellates To Bacterial Toxins That Produce Bleaching In Stony Corals, Diego L. Gil-Agudelo, Yael Ban-Haim, Eugene Rosenberg, Garriet W. Smith

Journal of the South Carolina Academy of Science

Bleaching of corals and other organisms with symbiotic zooxanthellae is a worldwide phenomenon with increasing importance due to global warming scenarios. Bleaching has been historically related to changes in the environment, especially water temperature increase, that stress corals and provoke the release of zooxanthellae. The discovery of Vibrio shilonii, a bacterium causing bleaching under thermal stress in corals of the Mediterranean Sea has changed our thinking about the cause (or explanation) for bleaching of corals worldwide. During this study, we evaluated the effect of a proline rich toxin, extracted from Vibrio shilonii, on zooxanthellae obtained from: Oculina patagonica from the …

Hierarchical Corannulene-Based Materials: Energy Transfer And Solid-State Photophysics, Allison M. Rice, W. Brett Fellows, Ekaterina A. Dolgopolova, Andrew B. Greytak, Aaron K. Vannucci, Mark D. Smith, Stavros G. Karakalos, Jeanette A. Krause, Stanislav M. Avdoshenko, Alexey A. Popov, Natalia B. Shustova Prof. Dr.

Faculty Publications

We report the first example of a donor–acceptor corannulene-containing hybrid material with rapid ligand-to-ligand energy transfer (ET). Additionally, we provide the first time-resolved photoluminescence (PL) data for any corannulene-based compounds in the solid state. Comprehensive analysis of PL data in combination with theoretical calculations of donor–acceptor exciton coupling was employed to estimate ET rate and efficiency in the prepared material. The ligand-to-ligand ET rate calculated using two models is comparable with that observed in fullerene-containing materials, which are generally considered for molecular electronics development. Thus, the presented studies not only demonstrate the possibility of merging the intrinsic properties of π-bowls, …

Data-Driven Identification Of Potential Zika Virus Vectors, Michelle V. Evans, Tad Dallas, Barbara A. Han, Courtney C. Murdock, John M. Drake

Faculty Publications

Zika is an emerging virus whose rapid spread is of great public health concern. Knowledge about transmission remains incomplete, especially concerning potential transmission in geographic areas in which it has not yet been introduced. To identify unknown vectors of Zika, we developed a data-driven model linking vector species and the Zika virus via vector-virus trait combinations that confer a propensity toward associations in an ecological network connecting flaviviruses and their mosquito vectors. Our model predicts that thirty-five species may be able to transmit the virus, seven of which are found in the continental United States, including Culex quinquefasciatus and Cx. …

Naturally-Occurring Changes In Social-Cognitive Factors Modify Change In Physical Activity During Early Adolescence, Rod K. Dishman, Marsha Dowda, Kerry L. Mciver, Ruth P. Saunders, Russell R. Pate

Faculty Publications

Purpose

To determine whether naturally-occurring changes in children’s motives and beliefs are associated with the steep decline in physical activity observed from childhood to early adolescence.

Methods

Latent growth modeling was applied in longitudinal tests of social-cognitive influences, and their interactions, on physical activity in a large cohort of boys and girls evaluated annually between 5th and 7th grades.

Results

Measurement equivalence of motives and beliefs was confirmed between boys and girls. After adjustment for gender and maturity differences, physical activity declined less in children who reported the least decreases in self-efficacy for overcoming barriers to activity and perceived parental …

Climate Warming Can Accelerate Carbon Fluxes Without Changing Soil Carbon Stocks, Susan E. Ziegler, Ronald Benner, Sharon A. Billings, Kate A. Edwards, Michael Philben, Xinbiao Zhu, Jerome Laganière

Faculty Publications

Climate warming enhances multiple ecosystem C fluxes, but the net impact of changing C fluxes on soil organic carbon (SOC) stocks over decadal to centennial time scales remains unclear. We investigated the effects of climate on C fluxes and soil C stocks using space-for-time substitution along a boreal forest climate gradient encompassing spatially replicated sites at each of three latitudes. All regions had similar SOC concentrations and stocks (5.6 to 6.7 kg C m−2). The three lowest latitude forests exhibited the highest productivity across the transect, with tree biomass:age ratios and litterfall rates 300 and 125% higher than those in …

The Slow Food Movement: A 'Big Tent' Ideology, Mark N. Wexler, Judy Oberlander, Arjun Shankar

Journal of Ideology

The Slow Food Movement (SFM) has been characterized as a reminder of the centrality of the kitchen in the “good old days”. It has also been referred to as a poor use of science posing as a rallying point for the beleaguered middle class trying to reclaim psychological territory lost to the fast-paced commercialization of what was once private or leisure time. This paper argues that both these criticisms contain more than a kernel of truth. However, each misses the mark when it comes to explaining the relative successes of SFM. In this paper, we explore SFM’s basic ideological premises. …

Individual Fluid Plans Versus Ad Libitum On Hydration Status In Minor Professional Ice Hockey Players, Dawn M. Emerson, Toni Torres-Mcgehee, Charles C. Emerson, Teri L. Lasalle

Faculty Publications

BACKGROUND: Despite exercising in cool environments, ice hockey players exhibit several dehydration risk factors. Individualized fluid plans (IFPs) are designed to mitigate dehydration by matching an individual's sweat loss in order to optimize physiological systems and performance. METHODS: A randomized control trial was used to examine IFP versus ad libitum fluid ingestion on hydration in 11 male minor professional ice hockey players (mean age = 24.4 ± 2.6 years, height = 183.0 ± 4.6 cm, weight = 92.9 ± 7.8 kg). Following baseline measures over 2 practices, participants were randomly assigned to either control (CON) or intervention (INT) for 10 …

Altered Gut Microbiome In A Mouse Model Of Gulf War Illness Causes Neuroinflammation And Intestinal Injury Via Leaky Gut And Tlr4 Activation, Firas Alhasson, Suvarthi Das, Ratanesh K. Seth, Diptadip Dattaroy, Varun Chandrashekaran, Caitlin N. Ryan, Luisa S. Chan, Traci Testerman, James Burch, Lorne J. Hofseth, Ronnie Horner, Mitzi Nagarkatti, Prakash Nagarkatti, Stephen M. Lasley, Saurabh Chatterjee

Faculty Publications

Many of the symptoms of Gulf War Illness (GWI) that include neurological abnormalities, neuroinflammation, chronic fatigue and gastrointestinal disturbances have been traced to Gulf War chemical exposure. Though the association and subsequent evidences are strong, the mechanisms that connect exposure to intestinal and neurological abnormalities remain unclear. Using an established rodent model of Gulf War Illness, we show that chemical exposure caused significant dysbiosis in the gut that included increased abundance of phylum Firmicutes and Tenericutes, and decreased abundance of Bacteroidetes. Several gram negative bacterial genera were enriched in the GWI-model that included Allobaculum sp. Altered microbiome caused significant decrease …

Air- Sea Interactions And Ocean Dynamics In The Southwest Tropical Indian Ocean, Jessica Maureen Burns

Theses and Dissertations

The Southwest Tropical Indian Ocean (SWTIO) features a unique, seasonal upward lift of the thermocline, which is known as the Seychelles-Chagos Thermocline Ridge (SCTR; 55°E-65°E, 5°S-12°S). It is known that a high correlation exists between the depth of the thermocline and sea surface temperature (SST; a key ingredient for tropical cyclogenesis). With a particular focus on 2012/2013, this study reveals the dynamic properties of the SCTR that play an important role in the modulation of tropical cyclones in the SWTIO. Phenomena including Indian Ocean Dipole (IOD) and El Niño Southern Oscillation (ENSO) are also well correlated to cyclogenesis through changes …

Mismatch Tolerance During Homologous Recombination In Mammalian Cells, Shen Li

Theses and Dissertations

Homologous recombination (HR) serves critical roles in DNA repair to maintain genome stability, and the malfunction of HR contributes to carcinogenesis and cancer development. Current research focuses on the regulation against deleterious recombination between imperfectly matching sequences, which has been documented in certain myeloid leukemias, hereditary nonpolyposis colorectal cancers and other genetic diseases. Homology dependency of recombination was examined in cultured thymidine kinase-deficient mouse fibroblasts. Cells have chromosomal integration of DNA constructs harboring a herpes tk gene (the “recipient”) and a closely linked truncated “donor” tk sequence. The recipient was rendered non-functional by insertion of the recognition site for endonuclease …

The Influence Of Phytoplankton Size And Community Composition On Carbon Cycling And Planktonic Food Webs In The Sargasso Sea, Bridget Elise Cotti-Rausch

Theses and Dissertations

The Sargasso Sea is a dynamic physical environment located in the western North Atlantic where strong seasonal variability combines with forcing by mesoscale (~100 km) eddies. These drivers determine nutrient, light, and temperature regimes, and ultimate the size, composition and productivity of the phytoplankton community. My general objective was to determine how the structure and function of planktonic communities affected carbon export from the surface ocean in the Sargasso Sea. On four cruises (2011 and 2012; one eddy per cruise), I investigated links between water column structure, plankton community composition, size, and primary production (PP). I then combined PP data …

The Nutrition Assistance Landscape In Afterschool Programs: Understanding The Gap Between Research, Policy, And Practice, Falon Elizabet Tilley

Theses and Dissertations

The Child and Adult Care Food Program (CACFP) aims to increase children’s access to healthful foods in out-of-home care. Approximately half of the 10.2 million children enrolled in afterschool programs (ASPs) are eligible for nutrition assistance through programs like CACFP. Despite awareness of its potential impact on the nutritional status of millions of children, CACFP is underutilized among ASPs and notably understudied in comparison to other federal nutrition assistance programs.

This dissertation addresses key gaps in CACFP research and provides a deeper understanding of the nutrition assistance landscape in afterschool programs by (1) assessing the type of afterschool snacks served …

Molecular Exploration Of Bioavailable Dissolved Organic Matter Across Aquatic Ecosystems, Yuan Shen

Theses and Dissertations

Dissolved organic matter (DOM) in aquatic ecosystems is a large reservoir of reduced carbon that is mostly resistant to degradation. A small fraction of DOM cycles relatively quickly and is biologically utilized on timescales of days to months. This bioavailable DOM (BDOM) supports aquatic food webs, drives major elemental cycles, and is coupled to atmospheric CO2. Despite wide-ranging importance, bioavailability of DOM and its linkages to ecosystem properties (e.g., primary production, nutrients) are poorly characterized, particularly at the ecosystem level. Bioassay experiments are commonly used to determine BDOM, but this approach alters conditions and has limited spatial and temporal coverage. …

The Role Of Exercise Dose On Ghrelin Concentration In Postmenopausal Women, Kimberly Bowyer

Theses and Dissertations

Introduction: As obesity continues to be a major public health concern, exercise is continually at the forefront of combating this epidemic. This thesis aims to examine how two different doses of exercise may affect the “hunger hormone”, ghrelin; in postmenopausal, non-obese women. Methods: 54 non-obese postmenopausal women were randomly assigned to either a higher dose (energy expenditure = 14 kilocalories/per kilogram of body weight/per week) or lower dose (energy expenditure = 8 kcal/kg/week) aerobic exercise training group for 16 weeks. Fasting blood samples were taken at baseline and at post intervention for analysis of ghrelin via multiplex immunoassay. Results: Only …

Mercury Bioaccumulation In Three Popular Subsistence And Recreational Estuarine Fishes From Southeastern U.S.A., Taylor Cannon

Theses and Dissertations

A rising concern in recent years has occurred over the presence of methylmercury in seafood, particularly fishes, and its impact on human health. However, fish also provide many health benefits, including improved cardiovascular health and neurodevelopment. Because fishes are the main source of methylmercury exposure to humans, and are such an integral part of the human diet, understanding the risks versus the benefits of fish consumption is imperative in allowing the public to make healthy, educated choices. One way that state and federal governments attempt to do this is through issuing fish consumption advisories. Unfortunately, these advisories do not always …

The Addition Of A Concurrent Bimanual Task Influences Postural Sway And Walking Speed Performance And Prioritization Across All Ages, Derek Matthew Liuzzo

Theses and Dissertations

As individuals age, there is an increase in attentional demands, a heavier reliance on vision, reduction in efficiency of both tasks, and longer processing intervals causing greater dual task interference on postural sway and walking speed. While cognitive-motor interference has been investigated, the impact of adding a functional, manual task while balancing or during gait is poorly understood across all ages. The overall purpose was to examine the relationship between age and task automaticity in dual-task conditions with a functional bimanual task, and describe how age influences attentional prioritization strategies. Older adults demonstrated differences in single and dual task performance …