Life Sciences Commons^™

Synthesis And Characterization Of Novel Platinum(Ii) And Platinum(Iv) Complexes Containing 4,4′--Disubstituted--2,2′--Bipyridine Ligands For The Treatment Of Cancer, Van Vo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Three series of platinum(II) and platinum(IV) complexes containing 4,4′-disubstituted-2,2′-bipyridine ligands have been synthesized and characterized by 1H NMR, 13C NMR spectroscopy, elemental analysis, mass spectroscopy, and differential scanning calorimetry measurements. The MTS cell proliferation assay was used to examine the in vitro anti-proliferative activities of these complexes in various human breast, lung, and prostate cancer cells. The cell's response to the complexes varies between different cell lines; however, the low EC₅₀ values determined from the MTS data indicate that several of the complexes are much more potent than cisplatin.

Flow cytometric analysis of selected compounds revealed induction of apoptosis …

Considering Sport Participation As A Source For Physical Activity Among Adolescents, Jennifer Pharr, Nancy L. Lough

Environmental & Global Health Faculty Research

BACKGROUND:

Studies have shown participation in sport is lower among girls than boys, decreases as students matriculate through high school, is lowest among Black and Hispanic girls and has a positive relationship with SES. With sport recognized as a contributor to physical activity and health in adolescents, consideration of diminishing rates of participation appears warranted. The purpose of this study was to identify patterns related to differences in self-reported sport participation between genders, ethnic groups, grades and SES.

METHODS:

This study was a cross-sectional, secondary analysis of data collected for a sport interest survey. All students in grades 8-11 attending …

Initial Characterization Of A Conserved Active Site Residue For The Cdc34 Ubiquitin Conjugating Enzyme, Arvin Akoopie

Honors College Theses

Ubiquitin-conjugating enzymes (E2s) covalently modify protein substrates with ubiquitins. The active site cysteine residues on E2s are essential for catalyzing the transfer of ubiquitin from the E2 active site onto the protein substrate, however there is a limited amount of information available concerning additional active site residues for E2s that may also participate in catalysis. Cdc34 is an essential E2 that has merited the lion’s share of attention for biochemical analysis of the E2 family. Previous phylogenetic analysis of Cdc34 amino acid sequences has identified an invariably conserved histidine residue close to the active site cysteine in the primary structure, …

Biomechanical Analysis Of Gait Kinetics Resulting From Use Of A Vacuum Socket On A Transtibial Prosthesis, Maria De Lourdes Ramos Gonzalez

Honors College Theses

The technology and design of lower limb prosthetics have evolved greatly since their introduction. The current study proposed to compare the effects of a conventional pin socket attachment and a vacuum socket attachment for a transtibial amputee. Whereas traditional measurements of gait utilize force plates and camera systems, this study made use of a small tri-axial load cell located at the base of the socket to measure the forces and moments exerted during a regular gait cycle. The hypothesis tested stated that a vacuum pump socket attachment, when compared to a non-vacuum (pin) socket, will have a significant effect on …

Salivary Alpha-Amylase As An Indicator Of Body Stress Following An Acute Session Of Repetitive Jumping, Shaelee Allen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Determining body stress levels is important when developing training programs for athletes and rehab facilities. Stress levels during exercise are commonly measured using heart rate, oxygen consumption (VO₂), and blood biomarkers. These collection measures involve training on the part of the data collector and the participant, are invasive, and themselves elicit some stress. The purpose of this study is to test whether a 10-min repetitive jumping session will cause a significant increase in salivary alpha-amylase, a potential stress biomarker. This study also looks to determine if a 20-min recovery is enough time for amylase levels to return to …

Prymnesium Parvum And Fish Kills In A Southern Nevada Man-~Made Reservoir, Tara Gregg

UNLV Theses, Dissertations, Professional Papers, and Capstones

The water chemistry of Lake Las Vegas (LLV), a Southern Nevada man-made reservoir, is influenced by both anthropogenic and natural sources. These sources affect the reservoir's water chemistry, which may promote harmful algal blooms (HABs) leading to massive fish kill events. Fish kills, caused by the golden algaePrymnesium parvum (P. parvum), continue to pose a threat in the reservoir. However, no effective treatments to controlP. parvumin large reservoirs such as LLV have been determined. This cross-sectional study evaluated important variables that may affectP. parvumHABs in LLV, including non-P. parvumbiomass (mg/m3), total zooplankton abundance (#/m3), temperature, TDS (salinity), mineral concentration (Ca, …

Pronghorn (Antilocapra Americana) Offspring Recruitment On The Carrizo Plain National Monument: Evaluating The Effects Of Low Population Density And Marginal Habitat Quality., Diego Ramirez Johnson

UNLV Theses, Dissertations, Professional Papers, and Capstones

Demographic fluctuation among ungulate populations is strongly linked to variability in recruitment. Rates of recruitment are subject to various forms of density-dependent and density-independent regulation. For species which benefit from the presence of conspecifics, reduced population density can decrease rates of recruitment and trigger a decline in per capita growth. Termed the Allee effect, this scenario can cause demographic collapse and population extinction. For many ungulate species, predation on juveniles is reduced when the timing and distribution of births is synchronized within a local population. Because birth synchrony is density-dependent, it may act as a mechanism for the Allee effect …

Understanding How Honey Bee Flight And Senescence Are Connected Through Oxidative Stress., Joseph Margotta

UNLV Theses, Dissertations, Professional Papers, and Capstones

The goal of this dissertation was to exploit the tractability of the honey bee (Apis mellifera) to understand how the physiological and cellular mechanisms that determine the onset and duration of senescence are shaped by behavioral development and behavioral intensity. These data reveal how behavior can damage cells and consequently limit lifespan. The honey bee represents the ideal model to address these factors because age, behavior, functional senescence, and lifespan are easily manipulated independently of each other while in its natural environment. I determined if there was a cause-effect relationship between honey bee flight and oxidative stress by comparing damage …

The Effects Of A Comprehensive Weight Management Program, Including Nutrition Education, Physical Activity, & Behavior Change Techniques On Weight Loss And Health-Related Behaviors, Christina M. Vergara Aleshire

UNLV Theses, Dissertations, Professional Papers, and Capstones

Obesity is a major risk factor for several chronic diseases including hypertension, type 2 diabetes, cardiovascular disease, postmenopausal breast cancer, colon cancer, pancreatic cancer and all-cause mortality due to the physiological and morphological stresses placed on the body (Britten, Cleveland, Koegel, Kuczynski, Nickols-Richardson, S. 2012; Wu, Gao, Chen, van Dam, 2009). The latest CDC survey reports the US population is at 35.8 percent overweight and 28.1 percent obese, with the State of Nevada following closely at 36.3 percent overweight and 26.2 percent obese. The condition of obesity is characterized by an overall excessive storage of adipose tissue and diseased metabolic …

Isolation And Characterization Of Paenibacillus Larvae Bacteriophages For Use As A Treatment Of American Foulbrood Disease In Honeybees, Diane Gerda Yost

UNLV Theses, Dissertations, Professional Papers, and Capstones

American foulbrood disease is a destructive honeybee illness cause by the bacterial pathogen, Paenibacillus larvae. Current methods of treatment are either destructive or do not completely eradicate the infection, and as such, undertaking further research to determine the effectiveness of alternate treatment methods is of consequence.

The therapeutic use of bacteriophage that are capable of lysing host bacterial cells, or phage therapy, is one such potential treatment. Phage are viruses that infect bacteria, and are generally very host-specific. As such, targeting a pathogen would require obtaining phage specific for P. larvae. Therefore, obtaining P. larvae phage requires screening environmental samples …

Determining The Presence Of Carbapenem Antibiotic Resistance In Clinical Isolates, Fidelis Uzoma Enyinnaya

UNLV Theses, Dissertations, Professional Papers, and Capstones

Carbapenems are β-lactam antibiotics reserved for the treatment of severe microbial infections, especially those targeting the Enterobacteriaceae. Introduced in the 1980s, carbapenems have been used successfully in hospitals, and in the 1990s resistance was discovered. Carbapenem resistance is conferred through the production of carbapenemases. In the U.S., the most common carbapenemase is Klebsiella pneumoniae carbapenemase (KPC). In 2012, the National Healthcare Safety Network reported a carbapenem resistance rate of 13.0% among Klebsiella pneumoniae infections, and indicated that the mortality rate associated with carbapenem resistant Enterobacteriaceae (CRE) infections ranged from 48.0-71.9%. According to the Food and Drug Administration (FDA), carbapenem resistance …

The Grass Shrimp, Palaemonetes, Pugio: Hypoxic Influences On Embryonic Development, Christensen C. Javier

UNLV Theses, Dissertations, Professional Papers, and Capstones

Grass shrimp,Palaemonetes pugio, can survive in brackish waters and estuarine ecosystems despite the frequent oscillations and fluctuations in salinity, temperature and oxygen. AdultP. pugiohave the ability to osmoregulate (Romney and Reiber 2011), change cardiac parameters to tolerate temperatures (not yet published, Mika and Reiber) and oxyregulate (Guadagnoli and Reiber 2013). Manipulation of cardiac parameters allows for these methods of regulation. However, cardiac contraction and internal convection of oxygen do not occur until later stages of embryonic development. Studies focused on these morphological and physiological advantages may provide further understanding of the regulatory mechanisms within grass shrimp embryos, larvae and adults. …

Health Hazards In Rental Housing: An Overview Of Clark County, Nevada, Amanda Nicole Sokolowsky

UNLV Theses, Dissertations, Professional Papers, and Capstones

A wide range of health conditions are associated with housing conditions, including asthma, respiratory infections, injuries, mental health issues, and lead poisoning. People in modern societies spend more than 90% of their time indoors, the vast majority of which is spent at home. Therefore, any new information on housing related health hazards in a population provides an opportunity for a new prevention program. Reports have shown that rental properties have more health related hazards than owner occupied housing, and rental properties are frequently occupied by low-income and minority individuals. This descriptive study intended to provide an overview of housing related …

Life History Tradeoffs And The Costs Of Reproduction In Arizona Bark Scorpions (Centruroides Sculpturatus), Michael Marie Webber

UNLV Theses, Dissertations, Professional Papers, and Capstones

Tradeoffs in life history evolution result from conflicts in the time and energy that can be simultaneously invested in activities such as growth, reproduction, and survival. Reproduction is an energetically costly activity for organisms, and is known to elicit alterations in the daily activity patterns of individuals. I investigated reproductive tradeoffs in the Arizona Bark Scorpion,Centruroides sculpturatus. To better understand the nature of tradeoffs in this species, I examined the influence of reproductive state on the predatory efficiency and thermal preference of reproductive females. Further, I examined the influence of reproductive activities (i.e. gestation) on the ability of reproductive females …

Development Of A Molecular Method For Detecting The Causative Agent Of Swimmer's Itch (Trichobilharzia) In Freshwater Ecosystems, Heidi Mcmaster

UNLV Theses, Dissertations, Professional Papers, and Capstones

Cercarial dermatitis, commonly known as swimmer's itch, is caused by penetration of larvae of the schistosome,Trichobilharzia, into the dermis and epidermis layers of the skin. Symptoms are characterized by painful swelling and itching at the site of penetration. The normal hosts for the life cycle of the schistosome are aquatic birds and aquatic snails. The most frequently used method of detection forTrichobilharziais microscopy. With increases in the occurrence of cercarial dermatitis outbreaks in freshwater in the Southwestern United States, it is becoming increasingly important to develop and standardize a molecular method for rapid detection that can assist health professionals and …

Evaluating The Impact Of A Home-~Based Childhood Asthma Intervention Program In Clark County, Nevada, Mackenzie Suzanne Burns

UNLV Theses, Dissertations, Professional Papers, and Capstones

Asthma is a chronic, incurable, costly, and potentially life–threatening disease that affects an estimated 7 million children in the United States; further, more than 56,000 Nevada children are currently living with asthma. The literature suggests that a number of factors that contribute to either the development of asthma or the exacerbation of asthma symptoms in sensitive individuals can be traced to the home environment. Given that the majority of Americans spend over 90% of their time indoors, of which two–thirds are spent at home, a home–based childhood asthma intervention program represents a unique primary prevention opportunity. This pre–experimental study evaluated …

The Effects Of A Single Bout Of Self-~Myofascial Release On Flexibility And Maximum Voluntary Contraction, Jonathan Robert Garcia

UNLV Theses, Dissertations, Professional Papers, and Capstones

Purpose: The purpose of this study was to determine the effect of foam rolling on hamstrings flexibility, isometric and isokinetic torque. Participants: Thirteen female participants (N = 13; age: 28.5 + 6.8 years of age; height: 165.5 + 6.7 cm; mass: 64.2 + 8.4 kg) volunteered to participate in the study.

Methods: Hamstring flexibility was assessed using a sit and reach test; muscle strength was assessed by having participants complete a maximal voluntary isometric contraction (MVIC) of the hamstrings and maximal effort isokinetic knee flexion test using an isokinetic dynamometer. Participants completed these tests prior to any intervention (pre-rolling 1), …

The Relationship Among School Playground Design And Conditions And Physical Activity Levels In Children, Ipuna Estavillo Black

UNLV Theses, Dissertations, Professional Papers, and Capstones

Almost 20% of children aged 6 to 11 years are obese in the United States, tripling over the last ten years. The rise in childhood obesity challenges nurses in their efforts to improve community health and sustainability. A decrease in physical activity (PA) levels has been associated with an increase in obesity. Schools have been identified as a primary setting to provide children adequate amounts of daily PA, and nurses working in the area of child health promotion can work with schools to provide opportunities for children to increase PA levels. The playground is one environment where children can increase …

Analysis Of The Morbidity And Mortality Of Severe Influenza Infection In Clark County, Nevada For The 2010-2011 Influenza Season, Kindra St. Jacques, Patricia Cruz, Brian Labus

Nevada Journal of Public Health

Throughout the duration of any influenza season, influenza strains have the ability to evolve through mutation causing alterations in virulence. These changes may result in severe illness or death among susceptible populations; therefore, it is important to closely monitor influenza-associated hospitalizations and deaths. The University of Nevada, Las Vegas in collaboration with the Southern Nevada Health District analyzed data from the hospitalized influenza morbidity and mortality surveillance project for Clark County for the 2010-2011 influenza season. Among the study population (N= 158): the influenza strain type was found to be significantly associated with deaths (n= 25), vaccination status was not …

Environmental Correlates Of Physical Activity Among African-American Adults, Tim Bungum, Jennifer Pharr, Paulo S. Pinheiro, Maria Azzarelli

Nevada Journal of Public Health

The purpose of this cross-sectional study was to identify perceived environmental correlates of meeting physical activity guidelines among African-American adults living in Southern Nevada. Trained interviewers phoned potential participants who lived in the 12 zip codes of Clark County, Nevada with the highest proportions of African-American residents. Respondents (n=237) answered 52 health-related and demographic questions. Slightly less than 50% of participants met physical activity guidelines. A factor analysis procedure produced two environmental variables, neighborhood safety and environmental supports for physical activity. Age, gender and educational attainment (p<.05) predicted the meeting of those guidelines (R2=.214), while neighborhood support for physical activity, neighborhood safety, and BMI failed to do so. This finding suggests that environmental factors are not strong predictors of physical activity among African- American adults, although environmental supports for physical activity approached significance. Future studies should consider assessing additional aspects of the built environment as an influence on physical activity.

Understanding Racial Hiv/Sti Disparities In Black And White Men Who Have Sex With Men: A Multilevel Approach, Patrick S. Sullivan, John Peterson, Eli S. Rosenberg, Colleen F. Kelley, Hannah Cooper, Adam Vaughan, Laura F. Salazar, Paula M. Frew, Gina Wingood, Ralph Diclemente, Carlos Del Rio, Mark Mulligan, Travis H. Sanchez

Environmental & Global Health Faculty Research

Background The reasons for black/white disparities in HIV epidemics among men who have sex with men have puzzled researchers for decades. Understanding reasons for these disparities requires looking beyond individual-level behavioral risk to a more comprehensive framework. Methods and Findings

Religion, Partisanship, And Attitudes Toward Science Policy, Ted G. Jelen, Linda A. Lockett

Political Science Faculty Research

We examine issues involving science which have been contested in recent public debate. These “contested science” issues include human evolution, stem-cell research, and climate change. We find that few respondents evince consistently skeptical attitudes toward science issues, and that religious variables are generally strong predictors of attitudes toward individual issues. Furthermore, and contrary to analyses of elite discourse, partisan identification is not generally predictive of attitudes toward contested scientific issues.

Impacts And Management Of Hemlock Woolly Adelgid In National Parks Of The Eastern United States, Scott R. Abella

Life Sciences Faculty Research

No abstract provided.

Synthesis And Characterization Of Pt(Ii) Complexes For Anticancer Therapy, Mihaela A. Ciulei, Pradip K. Bhowmik

McNair Poster Presentations

The first platinum-based drug was discovered and approved by Food and Drug Administration (FDA) in 1978 is cis-diamminedichloroplatinum (II) (cisplatin or CDDP). Cisplatin is used for about 50% of the chemotherapeutic cancer treatments along with its two analogues carboplatin and oxaliplatin. So far these drugs have been used extensively as treatment for ovarian, bladder, head and neck, and lung cancers. Although cisplatin has been used so often, it has toxic side effects and drug resistance.1-4 Due to these limitations other compounds have been synthesized. Specifically, our lab in conjunction with a biochemistry lab has recently published one article …

Modulatory Effects Of Gaba(B) Receptor Facilitation In A Model Of Chronic Inflammation, Michael A. Langhardt, Jefferson Kinney

McNair Poster Presentations

Inflammation within the brain (neuroinflammation) has been associated with a number of neurodegenerative diseases, including Alzheimerʼs disease (AD) (Solito et al., 2012). Within the brain, inflammation is defined broadly as prolonged activation of the brainʼs immune cells, known as glial cells. Excessive activation of glial cells within the brains of AD patients is a hallmark of the disease, however the mechanism by which this contributes to disease pathology is relatively unclear (Jo et al., 2014). Recently, studies have shown that glial cells, known as astrocytes, are able to synthesize and release the inhibitory neurotransmitter GABA (Charles et al., 2003). Further, …

Effects Of Anti-Inflammatory Foods On Hyperglycemia In Type-1 Diabetics, Primrose Martin, Michelle Chino

McNair Poster Presentations

Based on a case study administered by Dr. Charlotte Gerson, an IDDM patient referred to as R.H. reversed hyperglycemic symptoms within ten weeks while adhering to a diet of mainly vegetables and some low-glycemic index fruits. R.H. also ingested mineral supplements and underwent multiple coffee enemas each day (Gerson & Bishop, 2007, p. 56). This self-study hypothesizes that diet alone has a positive correlation coefficient with hyperglycemic blood glucose levels in type-1 diabetics (IDDM). Conducting this self-study contributes information to the limited research and database of alternative methods for managing IDDM hyperglycemia, and provides candid documentation of the effects that …

Runner Identity And Sponsorship: Evaluating The Rock ‘N’ Roll Marathon, Nancy L. Lough, Jennifer Pharr, Jason O. Owen

Environmental & Global Health Faculty Research

The economic value of participation sport has been reported to eclipse spectator sport significantly. However, scholars have acknowledged the relative lack of research on this important segment of the sport market. The purpose of this study was to analyze the relationship between runner identity and race sponsor effectiveness. Surveys were sent to participants in the Las Vegas Rock ‘n’ Roll Marathon. The survey was constructed to measure runner identity, and sponsor effectiveness as interpreted through rates of recognition, recall and purchase intention. Runners were divided into three groups based on their runner identity score. Of the predictive variables, only runner …

Sex Determination Using Discriminant Function Analysis In Hispanic Children And Adolescents: A Lateral Cephalometric Study, Alyssa E. Sprowl

UNLV Theses, Dissertations, Professional Papers, and Capstones

Lateral cephalometric radiographs have been used for years to help diagnose skeletal and dental patterns in Orthodontics. Within the last decade, these radiographs have caught the interest of the department of Anthropology for the identification of gender within the adult and adolescent population. Numerous publications have been made but failed to identify sexual dimorphism in the pre-adolescent population. 303 lateral cephalograms of pre and post Latino adolescence age ranging from 6.5 to 17.9 years old were obtained from University of Nevada, Las Vegas (UNLV) digital database. 25 variables were identified and plotted with all linear and angular measurements transferred into …

Assessing Growth Response To Climate Controls In A Great Basin Artemisia Tridentata Plant Community, Lorenzo F. Apodaca

UNLV Theses, Dissertations, Professional Papers, and Capstones

An assessment of the growth response of key vegetative species to climatic variability is vital to identifying possible local impacts on ecosystems faced with imminent climate change. With current climate projections in Nevada predicting a shift to an even more arid climate with greater year-to-year variability, the imperative exists to identify the effects of specific climatic controls on plant growth and to research methods to assess large-scale vegetative changes, especially in more remote areas where readily available data sets may be lacking. This study utilized annual growth ring indices constructed from big sagebrush (Artemisia tridentatassp.tridentata) stems collected in Spring Valley, …

Evaluation Of The Relationship Between Nutrition Knowledge And Disordered Eating Risk In Female Collegiate Athletes, Amy L. Miracle

UNLV Theses, Dissertations, Professional Papers, and Capstones

Involvement in collegiate sports among female athletes is at an all-time high with approximately 198,000 currently participating, (National Collegiate Athletic Association, 2012). With this increase in female participation in collegiate sports, the pressures of sport participation and academics lead some women to engage in harmful eating behaviors. These harmful eating behaviors may lead to the development of disordered eating (C. Johnson, Powers, & Dick, 1999; Quatromoni, 2008; Reel, SooHoo, Petrie, Greenleaf, & Carter, 2010; Reinking & Alexander, 2005). Many factors have been identified as contributing to the development of disordered eating but nutrition knowledge or lack thereof, has yet to …