Life Sciences Commons^™

Leaf Micromorphology Aids Taxonomic Delineation Within The Hypervariable Genus Metrosideros (Myrtaceae) On O'Ahu, Gary L. Sur, Richard Keating, Elizabeth Stacy

Life Sciences Faculty Research

Variation in leaf micromorphology can aid delimitation of taxonomically difficult groups. The woody genus Metrosideros (Myrtaceae) dominates Hawai'i's native forests and comprises striking, predominantly infraspecific, macromorphological variation, including many forms unrecognized in current taxonomic treatments. On taxonomically rich O'ahu, 10 taxa (varieties or unnamed morphotypes of M. polymorpha and other species) occur in a predictable sequence with overlapping ranges from low to high elevation along the numerous leeward ridges of the Ko'olau Range. We used scanning electron and light microscopy to examine mature, nonsenescing sun leaves from each of these 10 taxa. Parametric and nonparametric tests were used to compare …

Variation In Airborne Pollen Concentrations Among Five Monitoring Locations In A Desert Urban Environment, Tanviben Y. Patel, Mark P. Buttner, David Rivas, Chad L. Cross, Dennis A. Bazylinski, Joram S. Seggev

Environmental & Global Health Faculty Research

The urbanization of the Las Vegas Valley has transformed this part of the Mohave Desert into a green oasis by introducing many non-native plant species, some of which are allergenic. Typically, one monitoring station is established per city to obtain pollen counts for an entire metropolitan area. However, variations in pollen concentrations could occur among different microenvironments. The objective of this study is to measure and compare pollen concentrations in five locations in Las Vegas to determine if there are significant differences between microenvironments within the city. Air samples were collected from five sites across the Las Vegas Valley over …

Knee Joint Kinematics And Kinetics During Walking And Running After Surgical Achilles Tendon Repair, Daniel Jandacka, Jan Plesek, Jiri Skypala, Jaroslav Uchytil, Julia Freedman Silvernail, Joseph Hamill

Kinesiology and Nutrition Sciences Faculty Research

Background: Despite the increasing incidence of Achilles tendon (AT) ruptures, there is a lack of information on the possible risks associated with regular running and walking for exercise after an injury. There are some known kinematic gait changes after an AT rupture, especially at the knee. However, it is not clear whether runners with AT ruptures may be at risk for secondary knee injuries during shod or barefoot running/walking. Purpose/Hypothesis: The purpose of this study was to compare the kinematics and kinetics of barefoot walking and barefoot and shod running between athletes with a history of AT ruptures and a …

Complete Genome Sequences Of Paenibacillus Larvae Phages Bn12, Dragolir, Kiel007, Leyra, Likha, Pagassa, Pbl1c, And Tadhana, Jamison K. Walker, Bryan D. Merrill, Jordan A. Berg, Aziza Dhalai, Douglas W. Dingman, Chris P. Fajardo, Kiel Graves, Hunter L. Hill, Jared A. Hilton, Cameron Imahara, Bradley K. Knabe, James Mangohig, Josh Monk, Heejin Mun, Ashley M. Payne, Alicia Salisbury, Casey Stamereilers, Kathie Velez, Andy T. Ward, Donald P. Breakwell, Julianne H. Grose, Sandra Hope, Philippos K. Tsourkas

Life Sciences Faculty Research

We present here the complete genomes of eight phages that infect Paenibacillus larvae, the causative agent of American foulbrood in honeybees. Phage PBL1c was originally isolated in 1984 from a P. larvae lysogen, while the remaining phages were isolated in 2014 from bee debris, honeycomb, and lysogens from three states in the USA.

Onward And Upward: Optimizing Motor Performance, Lee-Kuen Chua, Gabriele Wulf, Rebecca Lewthwaite

Kinesiology and Nutrition Sciences Faculty Research

In the OPTIMAL theory of motor learning (Wulf & Lewthwaite, 2016), three factors are postulated to facilitate motor performance and learning: Enhanced expectancies (EE) for performance, autonomy support (AS), and an external focus (EF) of attention. We examined whether EE, AS, and EF would have immediate performance benefits and whether implementing these factors consecutively would lead to incremental performance increases. Participants were assigned to the optimized or control groups and performed a maximal jump. After the first trial block (baseline), optimized group participants were provided different conditions on each of the following 3 blocks: (a) Positive social-comparative feedback (EE); (b) …

The Acute Effects Of Cupping Therapy On Hamstring Range Of Motion Compared To Sham, Matthew Schafer

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Flexibility is an important aspect of physical performance and when deficient can result in an increased opportunity for injury. Cupping therapy is an ancient technique that has recently seen a growth in popularity in Western Orthopedic medicine as a soft tissue mobilization technique. Most cupping therapy research explores the use of cupping therapy for treating headache, herpes zoster, asthma, cough, and other non-orthopedic pathologies. Cupping therapy has had positive results on an injured population for increasing flexibility. Objective: To identify if cupping therapy applied passively for 10 minutes results in an increase in flexibility, and to identify if there …

Biomechanical Analysis Of Jumping: The Influence Of External Load And Countermovement Depth On Deceleration Strategies And Performance, Leland Adam Barker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Jumping performance has traditionally been measured by jump height alone. In recent years, the reactive strength index (RSI = Jump height / jump time)) has been used as another measure of jump performance. According to RSI, which was developed to assess eccentric force production, jump performance can improve by increasing jump height, decreasing jump time, or both simultaneously. However, it is not clear how force production correlates to RSI variables. If RSI is meant to be a practical measure of eccentric force production, it should correlate strongly to eccentric and amortization force production during jumping. Thus, the purpose of the …

Transcriptional Silencing And Anti-Silencing Of Virulence Genes In The Bacterial Pathogen Shigella Flexneri: Virb, Dna Supercoiling, And The Histone-Like Nucleoid Structuring Protein, Michael Adam Picker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Transcriptional silencing and anti-silencing affect many aspects of bacterial physiology, including virulence in bacterial pathogens. In Shigella species, a group of gram-negative pathogens that cause bacillary dysentery in humans, the histone-like nucleoid structuring protein (H-NS) transcriptionally silences virulence genes found on the large virulence plasmid while VirB anti-silences these genes. However, the mechanistic details of their interplay are not fully understood. To elucidate their regulatory mechanisms, I use the icsP virulence locus, which shares a long intergenic region with the divergently transcribed ospZ gene (1535 bp from TSS to TSS). Prior to this work, two discrete H-NS binding regions had …

Does Distance Climbed In A 30-S Maximal Effort Test On A Simulated Climbing Machine Correlate With Wingate Variables?, Kayla Wright

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: The Wingate Anaerobic Test (WAnT) is used to assess anaerobic capacity by measuring total work. In addition to total work, the WAnT measures peak power, mean power, relative peak power, and fatigue. There are lower-, upper-, and full-body alternatives to the WAnT that can be used to measure anaerobic capacity, but there is little data on these alternatives. It may be beneficial to have a full-body assessment of anaerobic capacity, as many sporting events have full-body anaerobic demands. Simulated climbing machines are becoming popular modes of exercise. They have been compared to treadmill running and cycling in terms of …

Effect Of Exercise And Hypoxia On Plasma Telomerase, Charli D. Aguilar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Telomerase reverse transcriptase (TERT) is the enzyme that adds telomeric sequences to the end of linear chromosomes. Exercise has shown to upregulate acutely leukocyte TERT after just 30 minutes of running on a treadmill at 80% of VO2max (Denham et al., 2016). Hypoxia inducible factor 1 (HIF-1) is also a mediator of TERT in in vitro (Nishi et al., 2004). Moderate acute exposure to hypoxia was associated with substantial increases in plasma TERT in a recent study on rats (Wang et al., 2014). The specific aim of the current study was to identify if acute hypoxia upregulates plasma TERT …

Money Matters: Exploring The Financial Resources For Sports Medicine Programs In Public And Private Secondary Schools--A Qualitative Study, Natalie Mercedes Aviles Flores

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Institutional funding and strained school budgets are a barrier to placing certified athletic trainers (ATCs) in secondary schools. The size and location of a school appear to be coinciding limitations (Mazerolle et al, 2015), but what is lacking in the literature is an

evaluation of how schools manage to provide adequate medical coverage. Resources are available, but there is question as to whether these schools consider or even utilize them.

Objective: The aim of this qualitative study is to explore the funding techniques and budget sources of high school athletic training clinics from the perspective of both private and …

Movement Efficiency Through Autonomy Support, Takehiro Iwatsuki

UNLV Theses, Dissertations, Professional Papers, and Capstones

Supporting performer autonomy has been consistently been shown to enhance motor learning (for reviews, see Sanli, Patterson, Bray, & Lee, 2013; Wulf, 2007; Wulf & Lewthwaite, 2016). Autonomy-supportive situations are those in which learners are given control over aspects of the practice conditions or are provided with other choices, including small and incidental choices that are not necessarily related to the task at hand. Providing autonomy support also benefits immediate motor performance, as demonstrated by enhanced punching velocity and impact forces in a study involving skilled kick boxers (Halperin, Chapman, Martin, Lewthwaite, & Wulf, 2016). Autonomy support is a key …

Effect Of Bmp Treatment On Periostin Gene Expression In Pre-Osteoblastic Mc3t3-E1 Mouse Cells, Vincent Lee Khang

UNLV Theses, Dissertations, Professional Papers, and Capstones

Periostin is a secreted, extracellular matrix (ECM) protein widely expressed within collagen-rich fibrous connective tissues of the body including the periodontal ligament (PDL), bone, skin, heart, and cornea. Periostin has been shown to serve many important regulatory functions including cell adhesion, cell motility, wound healing and of particular importance to the dental field, differentiation of osteoblasts. The deletion of periostin compromises osteoblast attachment to bone matrix and induces a reduction in mineralization and expression of bone markers, including type I collagen, osteocalcin, osteopontin and alkaline phosphatase. Periostin has also been shown to play a significant role in collagen fibrillogenesis by …

Changes In Igf-1 Levels Post Deer Antler Velvet Supplementation, Nicole Alexandra Marmillo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Deer antler velvet has been used in traditional Chinese medicine for thousands of years but has recently gained popularity in Western medicine as an ergogenic aid. According to Oriental medicine deer antler velvet can be attributed to enhancing immune system function, improving athletic performance, increasing muscle recovery, enhancing sexual function, improving disease recovery, and enhancing cardiovascular function. Deer antler velvet is orally taken in pill, powder or spray form. Although a number of health and human performance effects have been attributed to deer antler velvet the scientific rationale behind these beliefs is ambiguous Objective: The purpose of this study …

Larval-Ant Interactions In The Mojave Desert: Communication Brings Us Together, Alicia Mellor

UNLV Theses, Dissertations, Professional Papers, and Capstones

Butterflies are a diverse and essential group of pollinators whose abundance is predominantly determined by growth and survival of their larvae. In the family Lycaenidae, many species participate in a larval-ant mutualism where ants feed on nutrient-rich nectar produced by larvae and, in turn, protect those larvae from predators. Emerging evidence indicates larval scent in the form of cuticular hydrocarbons (CHCs), not nectar production, drives this interaction. This study takes two approaches to investigate CHCs as the driver behind the larval-ant mutualism in three butterfly species native to southern Nevada: Euphilotes bernardino martini (Martin’s blue), Brephidium exilis (western pygmy blue), …

Digging Biomechanics In Geomyoid Rodents, Alexis Moore Crisp

UNLV Theses, Dissertations, Professional Papers, and Capstones

Subterranean digging behaviors provide opportunities for protection, access to prey, and predator avoidance for a diverse array of vertebrates, yet studies of the biomechanics of burrowing have been limited by the technical challenges of measuring kinetics and kinematics of animals moving within a substrate. Prior studies of burrowing have recorded a single axis of x-ray video and/or force. However, empirical observations show that burrowing is not restricted to a single axis or plane. I describe a new system called a ‘tunnel-tube’ for measuring 3D reaction force during burrowing. This tunnel-tube has two separate tubes, one ‘entry tube’ that has no …

Prevalence Of Cariogenic Microbial Flora Among Scardovia Wiggsiae-Positive And Negative Patients, Amy Tam

UNLV Theses, Dissertations, Professional Papers, and Capstones

The formation of dental caries (cavities) is a complex, multi-dimensional process that necessarily involves many risk factors – including the acquisition and colonization of cariogenic oral bacteria. The most frequently associated oral pathogens are the acid-producing and acid-tolerant oral streptococcus species, such as Streptococcus mutans (S. mutans or SM). Many studies have established and confirmed the critical role of the formation of biofilm in the virulence of S. mutans, and the critical role this may play in determining the balance of the oral microbiome towards health or disease.

More recent efforts have discovered a novel cariogenic pathogen, Scardovia wiggsiae (S. …

An Examination Of The Community And Environmental Impact Of A Las Vegas Convention Food Rescue Program, Samantha H. To

UNLV Theses, Dissertations, Professional Papers, and Capstones

Food insecurity affects millions of Americans each year, inflicting negative consequences on the physical and mental health outcomes at all ages. Concurrently, millions of tons of leftover edible foods are sent to landfill each year. Not only does this pose a question of ethics, but increasingly harms the environment by misusing resources and increasing the release of

greenhouse gases.

Food rescue advisory meetings highlighted the need for more focused research to be conducted that assessed the impacts of donating rescued convention food on local environmental and community health. A mixed-method approach was used to detail the food rescue process between …

The Caspase Cascade During Hibernation In The Golden-Mantled Ground Squirrel, Spermophilus Lateralis, Michael David Treat

UNLV Theses, Dissertations, Professional Papers, and Capstones

In several human pathologies like heart attack, stroke, neurodegenerative diseases, and autoimmune disorders, widespread cell death, or apoptosis, is a major cause of organ dysfunction and death. Hibernating golden-mantled ground squirrels, Spermophilus lateralis, experience numerous conditions during the winter that are known to be pro-apoptotic in other mammal systems (e.g. extreme hypothermia, ischemia and reperfusion, acidosis, increased reactive oxygen species, bone and muscle disuse). However, studies suggest that hibernators may invoke a protective phenotype to limit widespread cell damage and loss during the hibernation season. Could regulating apoptosis provide protection against the harmful conditions experienced during the hibernation season? Could …

Screening For The Novel Cariogenic Pathogen Scardovia Wiggsiae Among Orthodontic Patients, Adam Whiteley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Screening for the Novel Cariogenic Pathogen Scardovia wiggsiae among Orthodontic Patients

by

Adam Whiteley

Dr. Karl Kingsley, Examination Committee Chair Professor of Biomedical Sciences University of Nevada, Las Vegas School of Dental Medicine

The recent discovery of a novel cariogenic pathogen Scardovia wiggsiae has led many scientists and oral health researchers to re-evaluate and re-examine existing saliva repositories to determine the prevalence among patient populations. Recent efforts at this institution have used existing saliva samples to determine the prevalence among both adults and pediatric patients. These studies have revealed this organism may be found in approximately one-quarter of all samples …

Application Of The Imb Model To The Reported Intake Of Fruits And Vegetables Of Native American Children, Rachel C. Sinley, Julie A. Albrecht

Journal of Health Disparities Research and Practice

The purpose of this study was to evaluate the applicability of the Information-Motivation-Behavioral Skills (IMB) model to identify variables that are associated with fruit and vegetable intake among Native American children. A cross-sectional study design was employed with a convenience sample of 92 English-speaking caregivers of Native American children between the ages of 2 and 5 from several tribes representing the Midwest, including Omaha, Santee Sioux, Ponca and Winnebago. Caregivers completed an IMB model survey, fruit and vegetable food frequency questionnaire and demographic survey. Multivariate linear regression and path analysis were conducted to assess association between model constructs and fruit …

Differential Tissue Response To Growth Hormone In Mice, Ryan Berry, Graham R. Mcginnis, Ronadip R. Banerjee, Martin E. Young, Stuart J. Frank

Kinesiology and Nutrition Sciences Faculty Research

Growth hormone (GH) has been shown to act directly on multiple tissues throughout the body. Historically, it was believed that GH acted directly in the liver and only indirectly in other tissues via insulin‐like growth hormone 1 (IGF‐1). Despite extensive work to describe GH action in individual tissues, a comparative analysis of acute GH signaling in key metabolic tissues has not been performed. Herein, we address this knowledge gap. Acute tissue response to human recombinant GH was assessed in mice by measuring signaling via phospho‐STAT5 immunoblotting. STAT5 activation is an easily and reliably detected early marker of GH receptor engagement. …

Evaluation Of The Antimicrobial Activity Of Endophytic Bacterial Populations From Chinese Traditional Medicinal Plant Licorice And Characterization Of The Bioactive Secondary Metabolites Produced By Bacillus Atrophaeus Against Verticillium Dahliae, Osama A. A. Mohamad, Li Li, Jin-Biao Ma, Shaimaa Hatab, Lin Xu, Jian-Wei Guo, Bakhtiyor A. Rasulov, Yong-Hong Liu, Brian P. Hedlund, Wen-Jun Li

Life Sciences Faculty Research

Endophytic bacteria associated with medicinal plants possess unique strategies that enhance growth and suvival of host plants, many of which are mediated by distinctive secondary metabolites. These bacteria and their secondary metabolites are important subjects for both basic and applied research aimed at sustainable agriculture. In the present study, 114 endophytic strains isolated from the wild ethnomedicinal plant Glycyrrhiza uralensis (licorice) were screened for their in vitro antimicrobial activities against common fungal pathogens of tomato (Fusarium oxysporum f. sp., Fulvia fulva, Alternaria solani), cotton (Fusarium oxysporum f. sp. Vesinfectum, Verticillium dahliae), pomegranite (Ceratocystis fimbriata), Cymbidinium (Colletotrichum gloeosporioides), and Tsao-ko (Pestalotiopsis …

Exertional Rhabdomyolysis After An Extreme Conditioning Competition: A Case Report, Ramires Alsamir Tibana, Nuno Manuel Frade De Sousa, Gabriel Veloso Cunha, Jonato Prestes, James W. Navalta, Fabricio Azevedo Voltarelli

Kinesiology and Nutrition Sciences Faculty Research

This case report describes an instance of exercise-induced rhabdomyolysis caused by an extreme conditioning program (ECP) competition. A 35-year-old female presented with abdominal pain and soreness, which began one day after she completed two days of ECPcompetition composed of five workouts. Three days after competition, creatine kinase (CK) was 77,590 U/L accompanied by myalgia and abnormal liver function tests, while renal function was normal and this resulted in a diagnosis of rhabdomyolysis. A follow-up examination revealed that her serum level of CK was still elevated to 3034 U/L on day 10 and 1257 U/L on day 25 following the ECP …

Differential Processing Of Nociceptive Input Within Upper Limb Muscles, Nathanial R. Eckert, Brach Poston, Zachary A. Riley

Kinesiology and Nutrition Sciences Faculty Research

The cutaneous silent period is an inhibitory evoked response that demonstrates a wide variety of responses in muscles of the human upper limb. Classically, the cutaneous silent period results in a characteristic muscle pattern of extensor inhibition and flexor facilitation within the upper limb, in the presence of nociceptive input. The aims of the current study were: 1) to primarily investigate the presence and characteristics of the cutaneous silent period response across multiple extensor and flexor muscles of the upper limb, and 2) to secondarily investigate the influence of stimulation site on this nociceptive reflex response. It was hypothesized that …

Surveillance On The Endemic Of Zika Virus Infection By Meteorological Factors In Colombia: A Population-Based Spatial And Temporal Study, Lung-Chang Chien, Ro-Ting Lin, Yunqi Liao, Francisco S. Sy, Adriana Pérez

School of Public Health Faculty Research

Background Zika virus (ZIKV) infection is a pandemic and a public health emergency. It is transmitted by mosquitoes, primarily the Aedes genus. In light of no treatment currently, it is crucial to develop effective vector control programs to prevent the spread of ZIKV infection earlier when observing possible risk factors, such as weather conditions enhancing mosquito breeding and surviving. Methods This study collected daily meteorological measurements and weekly ZIKV infectious cases among 32 departments of Colombia from January 2015–December 2016. This study applied the distributed lag nonlinear model to estimate the association between the number of ZIKA virus infection and …

Exploring The Use Of 3d Scanning To Determine Whole-Body Volume While Wearing A Triathlon Wetsuit, Leland Barker, Diego Medoza, John A. Mercer

Kinesiology and Nutrition Sciences Faculty Research

Background: Commercial 3 Dimension (3D) scanners are relatively new to anthropometry. The purpose of this study was to explore ability of using a 3D imaging instrument to measure body volume with and without wearing a wetsuit. Three experiments were conducted to achieve this purpose: (1) to determine if the 3D imaging instrument could accurately measure volume of static objects; (2) to determine the resolution of accuracy of measuring volume of static objects; and (3) to compare whole-body volume of wearing a wetsuit using 3D imaging as well as another body volume measure (air displacement technique). Methods: Three experiments were performed: …

A Biofilm Matrix-Associated Protease Inhibitor Protects Pseudomonas Aeruginosa From Proteolytic Attack, Boo Shan Tseng, Courtney Reichhardt, Gennifer E. Merrihew, Sophia A. Araujo-Hernandez, Joe J. Harrison, Michael J. Maccoss, Matthew R. Parsek

Life Sciences Faculty Research

Pseudomonas aeruginosa produces an extracellular biofilm matrix that consists of nucleic acids, exopolysaccharides, lipid vesicles, and proteins. In general, the protein component of the biofilm matrix is poorly defined and understudied relative to the other major matrix constituents. While matrix proteins have been suggested to provide many functions to the biofilm, only proteins that play a structural role have been characterized thus far. Here we identify proteins enriched in the matrix of P. aeruginosa biofilms. We then focused on a candidate matrix protein, the serine protease inhibitor ecotin (PA2755). This protein is able to inhibit neutrophil elastase, a bactericidal enzyme …

Assessing Near Surface Hydrologic Processes And Plant Response Over A 1600 M Mountain Valley Gradient In The Great Basin, Nv, U.S.A., Dale Devitt, Brian Bird, Brad Lyles, Lynn Fenstermaker, Richard Jasoni, Scotty Strachan, Jay Arnone Iii, Franco Biondi, Scott Mensing, Laurel Saito

Life Sciences Faculty Research

This study investigated near surface hydrologic processes and plant response over a 1600 m mountain-valley gradient located in the Great Basin of North America (Nevada, U.S.A.) as part of a long-term climate assessment study. The goal was to assess shifts in precipitation, soil water status and associated drainage with elevation and how this influenced evapotranspiration and plant cover/health estimated by a satellite-derived Normalized Difference Vegetation Index (NDVI), all to better understand how water is partitioned in a mountain valley system. Data were acquired during a three-year period from meteorological stations located in five plant communities ranging in elevation from 1756 …

Genomic Expansion Of Magnetotactic Bacteria Reveals An Early Common Origin Of Magnetotaxis With Lineage-Specific Evolution, Wei Lin, Wensi Zhang, Xiang Zhao, Andrew P. Roberts, Greig A. Paterson, Dennis A. Bazylinski, Yongxin Pan

Life Sciences Faculty Research

The origin and evolution of magnetoreception, which in diverse prokaryotes and protozoa is known as magnetotaxis and enables these microorganisms to detect Earth’s magnetic field for orientation and navigation, is not well understood in evolutionary biology. The only known prokaryotes capable of sensing the geomagnetic field are magnetotactic bacteria (MTB), motile microorganisms that biomineralize intracellular, membrane-bounded magnetic single-domain crystals of either magnetite (Fe3O4) or greigite (Fe3S4) called magnetosomes. Magnetosomes are responsible for magnetotaxis in MTB. Here we report the first large-scale metagenomic survey of MTB from both northern and southern hemispheres combined with 28 genomes from uncultivated MTB. These genomes …