Life Sciences Commons^™

Using A Mobile Application To Assess Knee Valgus In Healthy And Post-Anterior Cruciate Ligament Reconstruction Participants, Kai-Yu Ho, Brenda Benson Deaver, Tyrel Nelson, Catherine Turner

Physical Therapy Faculty Research

Context: Popularity of using handheld devices in clinical settings has increased, especially the use of motion analysis applications (MAAs). Video-based measurement tools have been found reliable in measuring knee valgus in subjects without anterior cruciate ligament (ACL) injury. However, there is a need for validation of using a MAA to measure knee valgus in an injured population, given that they may exhibit higher degrees of knee valgus. Objective: To examine the reliability and validity of using a MAA to measure knee valgus during functional activities used to assess return to sport after ACL reconstruction (ACLR). Design: Reliability and validity study. …

Molecular Specialization Of Gabaergic Synapses On The Soma And Axon In Cortical And Hippocampal Circuit Function And Dysfunction, April Contreras, Dustin J. Hines, Rochelle M. Hines

Psychology Faculty Research

The diversity of inhibitory interneurons allows for the coordination and modulation of excitatory principal cell firing. Interneurons that release GABA (γ-aminobutyric acid) onto the soma and axon exert powerful control by virtue of proximity to the site of action potential generation at the axon initial segment (AIS). Here, we review and examine the cellular and molecular regulation of soma and axon targeting GABAergic synapses in the cortex and hippocampus. We also describe their role in controlling network activity in normal and pathological states. Recent studies have demonstrated a specific role for postsynaptic dystroglycan in the formation and maintenance of cholecystokinin …

A 24-Week Double-Blind Placebo-Controlled Study Of The Efficacy And Safety Of The Ampa Modulator S47445 In Patients With Mild To Moderate Alzheimer's Disease And Depressive Symptoms, Eileen M. Bulger, Sylvie Gouttefangeas, Sylvie Bretin, Stéphanie Galtier, Philippe Robert, Vjera Holthoff-Detto, Jeffrey Cummings, Maria Pueyo

School of Medicine Faculty Research

Introduction S47445 is a novel positive allosteric modulator of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptors that may emerge as a favorable candidate for the symptomatic treatment of cognitive and depressive disorders in patients suffering from Alzheimer's disease (AD) of mild to moderate severity and with depressive symptoms. Methods For this double-blind, placebo-controlled 24-week phase II trial, 520 outpatients aged between 55 and 85 years, with probable AD at mild to moderate stages (a Mini-Mental State Examination score of 24-15 inclusive) and exhibiting depressive symptoms (Cornell Scale for Depression in Dementia [CSDD] ≥ 8) were recruited in twelve countries and randomized to 3 doses …

Associations Between Comorbid Tdp-43, Lewy Body Pathology And Neuropsychiatric Symptoms In Alzheimer's Disease, Ece Bayram, Guogen Shan, Jeffrey L. Cummings

School of Public Health Faculty Research

More than half of the patients with Alzheimer’s disease (AD) have comorbidities including TDP-43 and Lewy bodies, which are also associated with frontotemporal lobar degeneration and dementia with Lewy bodies, respectively. These comorbidities may help explain the overlapping neuropsychiatric symptoms between AD and other dementias. Data on 221 AD patients with Neuropsychiatric Inventory-Questionnaire were obtained from the National Alzheimer’s Coordinating Center. TDP-43 was associated with aberrant motor activity, whereas Lewy bodies were associated with anxiety, irritability, sleep behavior, and appetite problems. The associations between these comorbidities and neuropsychiatric symptoms were more significant for patients with sparse diffuse plaques.

Serum Apolipoproteins And Apolipopprotein-Defined Lipoprotein Subclasses: A Hypothesis-Generation Prospective Study Of Cardiovascular Events In Type 1 Diabetes, Arpita Basu Phd, Ionut Bebu Phd, Alicia J. Jenkins Md, Julie A. Stoner Phd, Ying Zhang Md; Phd, Richard L. Klein Phd, Maria F. Lopes-Virella Md; Phd, W. Timothy Garvey Md, Matthew J. Budoff Md, Peter Alaupovic Phd, Timothy J. Lyons Md

Kinesiology and Nutrition Sciences Faculty Research

Apolipoproteins and apolipoprotein-defined lipoprotein subclasses have been associated with dyslipidemia and cardiovascular disease (CVD). Our main objective was to define associations of serum apolipoproteins and ADLS with any CVD and major atherosclerotic cardiovascular events (MACE) in a prospective study of T1D. Serum apolipoproteins and ADLS (14 biomarkers in total) were measured in sera (obtained 1997-2000) from a subset (n=465) of the Epidemiology of Diabetes Interventions and Complications (EDIC) cohort. Prospective associations of any CVD (myocardial infarction, stroke, confirmed angina, silent MI, revascularization, or congestive heart failure) and MACE (fatal or nonfatal myocardial infarction or stroke), over 5942 and 6180 patient-years …

Equivalency Of Indirect And Direct Potable Reuse Paradigms Based On A Quantitative Microbial Risk Assessment Framework, Erfaneh Amoueyan, Yongsheng He, Hongjie Wu, Chuanwei Zhu, Shichun Huang, Jian Huang

Civil and Environmental Engineering and Construction Faculty Research

Due to rapid growth in the potable reuse industry, there is a need to better characterize the associated pathogen risks and degree of attenuation achieved by various treatment trains. It is also important to understand how emerging treatment frameworks compare to the historical practice of de facto reuse. As such, the goals of this study were to (1) evaluate the equivalency of indirect potable reuse (IPR) and direct potable reuse (DPR) systems; (2) compare alternative treatment trains; and (3) identify the design components and operational conditions that are most critical to minimizing public health risks. To this end, we developed …

Heterogeneity In Surface Sensing Suggests A Division Of Labor In Pseudomonas Aeruginosa Populations, Catherine R. Armbuster, Calvin K. Lee, Jessica Parker-Gilham, Jaime De Anda, Aiguo Xia, Kun Zhao, Keiji Murakami, Boo Shan Tseng, Lucas R. Hoffman, Fan Jin, Caroline S. Harwood, Gerard Cl Wong, Matthew R. Parsek

Life Sciences Faculty Research

The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP in response to surface adherence. Current thinking in the field is that once cells attach to a surface, they uniformly respond by producing c-di-GMP. Here, we describe how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells. One subpopulation has elevated c-di-GMP and produces biofilm matrix, serving as the founders of initial microcolonies. The other subpopulation has low c-di-GMP and engages in …

Novel Algorithms For Ldd Motif Search, Peng Xiao, Martin Schiller, Sanguthevar Rajasekaran

Life Sciences Faculty Research

Background: Motifs are crucial patterns that have numerous applications including the identification of transcription factors and their binding sites, composite regulatory patterns, similarity between families of proteins, etc. Several motif models have been proposed in the literature. The (l,d)-motif model is one of these that has been studied widely. However, this model will sometimes report too many spurious motifs than expected. We interpret a motif as a biologically significant entity that is evolutionarily preserved within some distance. It may be highly improbable that the motif undergoes the same number of changes in each of the species. To address this issue, …

Muscular Apoptosis But Not Oxidative Stress Increases With Old Age In A Long-Lived Diver, The Weddell Seal, Kaitlin N. Allen, Jose Pablo Vazquez-Medina, John M. Lawler, Jo-Ann E. Mellish, Markus Horning, Allyson G. Hindle

Life Sciences Faculty Research

Seals experience repeated bouts of ischemia–reperfusion while diving, potentially exposing their tissues to increased oxidant generation and thus oxidative damage and accelerated aging. We contrasted markers of oxidative damage with antioxidant profiles across age and sex for propulsive (longissismus dorsi) and maneuvering (pectoralis) muscles of Weddell seals to determine whether previously observed morphological senescence is associated with oxidative stress. In longissismus dorsi, old (age 17–26 years) seals exhibited a nearly 2-fold increase in apoptosis over young (age 9–16 years) seals. There was no evidence of age-associated changes in lipid peroxidation or enzymatic antioxidant profiles. In pectoralis, 4-hydroxynonenal-Lys (4-HNE-Lys) levels increased …

The Anti-Sigma Factor Muca Of Pseudomonas Aeruginosa: Dramatic Differences Of A Muca22 Vs. A Δmuca Mutant In Anaerobic Acidified Nitrite Sensitivity Of Planktonic And Biofilm Bacteria In Vitro And During Chronic Murine Lung Infection, Warunya Panmanee, Shengchang Su, Michael J. Schurr, Gee W. Lau, Xiaoting Zhu, Zhaowei Ren, Cameron T. Mcdaniel, Long J. Lu, Dennis E. Ohman, Daniel A. Muruve, Ralph J. Panos, Hongwei D. Yu, Thomas B. Thompson, Boo Shan Tseng, Daniel J. Hassett

Life Sciences Faculty Research

Mucoid mucA22 Pseudomonas aeruginosa (PA) is an opportunistic lung pathogen of cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) patients that is highly sensitive to acidified nitrite (A-NO2-). In this study, we first screened PA mutant strains for sensitivity or resistance to 20 mM A-NO2- under anaerobic conditions that represent the chronic stages of the aforementioned diseases. Mutants found to be sensitive to A-NO2- included PA0964 (pmpR, PQS biosynthesis), PA4455 (probable ABC transporter permease), katA (major catalase, KatA) and rhlR (quorum sensing regulator). In contrast, mutants lacking PA0450 (a putative phosphate transporter) and PA1505 (moaA2) were A-NO2- resistant. However, …

Ecological Effects Of Fear: How Spatiotemporal Heterogeneity In Predation Risk Influences Mule Deer Access To Forage In A Sky‐Island System, Christopher Lowrey, Kathleen M. Longshore, David M. Choate, Jyoteshwar R. Nagol, Joseph Sexton, Daniel Thompson

Life Sciences Faculty Research

Forage availability and predation risk interact to affect habitat use of ungulates across many biomes. Within sky‐island habitats of the Mojave Desert, increased availability of diverse forage and cover may provide ungulates with unique opportunities to extend nutrient uptake and/or to mitigate predation risk. We addressed whether habitat use and foraging patterns of female mule deer (Odocoileus hemionus) responded to normalized difference vegetation index (NDVI), NDVI rate of change (green‐up), or the occurrence of cougars (Puma concolor). Female mule deer used available green‐up primarily in spring, although growing vegetation was available during other seasons. Mule deer and cougar shared similar …

Acid Sphingomyelinase Regulates The Localization And Trafficking Of Palmitoylated Proteins, Xiahui Xiong, Chia-Fang Lee, Wenjing Li, Jiekai Yu, Linyu Zhu, Yongsoon Kim, Hul Zhang, Hong Sun

Chemistry and Biochemistry Faculty Research

In human, loss of acid sphingomyelinase (ASM/SMPD1) causes Niemann–Pick disease, type A. ASM hydrolyzes sphingomyelins to produce ceramides but protein targets of ASM remain largely unclear. Our mass spectrometry-based proteomic analyses have identified >100 proteins associated with the ASM-dependent, detergent-resistant membrane microdomains (lipid rafts), with >60% of these proteins being palmitoylated, including SNAP23, Src-family kinases Yes and Lyn, and Ras and Rab family small GTPases. Inactivation of ASM abolished the presence of these proteins in the plasma membrane, with many of them trapped in the Golgi. While palmitoylation inhibitors and palmitoylation mutants phenocopied the effects of ASM inactivation, we demonstrated …

Acc Theta Improves Hippocampal Contextual Processing During Remote Recall, Ryan A. Wirt, James M. Hyman

Psychology Faculty Research

Consolidation studies show that, over time, memory recall becomes independent of the medial temporal lobes. Multiple lines of research show that the medial frontal cortex, including the anterior cingulate cortex (ACC), is involved with contextual information processing and remote recall. We hypothesize that interactions between the ACC and hippocampal area CA1 will change as memories became more remote. Animals are re-exposed to multiple environments at different retention intervals. During remote recall, ACC-CA1 theta coherence increases, with the ACC leading area CA1. ACC theta regulates unit spike timing, gamma oscillations, and ensemble and single-neuron information coding in CA1. Over the course …

Microbiome Profiles Of Ligature-Induced Periodontitis In Nonhuman Primates Across The Life Span, Sreenatha Kirakodu, Jin Chen, Janis Gonzalez Martinez, Octavio A. Gonzalez, Jeffrey Ebersole

Dental Medicine Faculty Research

This investigation compared the microbiomes colonizing teeth during the initiation, progression, and resolution of periodontitis in nonhuman primates (Macaca mulatta) at different ages. Subgingival plaque samples were collected at baseline; 0.5, 1, and 3 months following ligature-induced periodontitis; and following naturally occurring disease resolution at 5 months. Samples were analyzed using 16S amplicon sequencing to identify bacterial profiles across age groups: young ( 3 years of age), adolescent (3 to 7 years), adult (12 to 15 years), and aged (17 to 23 years).-Diversity of the microbiomes was greater in the adult/aged samples than in the young/adolescent samples.-Diversity of the samples …

On The Origin Of Microbial Magnetoreception, Wei Lin, Joseph L. Kirschvink, Greig A. Paterson, Dennis A. Bazylinski, Yongxin Pan

Life Sciences Faculty Research

A broad range of organisms, from prokaryotes to higher animals, have the ability to sense and utilize Earth's geomagnetic field—a behavior known as magnetoreception. Although our knowledge of the physiological mechanisms of magnetoreception has increased substantially over recent decades, the origin of this behavior remains a fundamental question in evolutionary biology. Despite this, there is growing evidence that magnetic iron mineral biosynthesis by prokaryotes may represent the earliest form of biogenic magnetic sensors on Earth. Here, we integrate new data from microbiology, geology and nanotechnology, and propose that initial biomineralization of intracellular iron nanoparticles in early life evolved as a …

Cardio-Metabolic Disease Risk Factors Among South Asian Labour Migrants To The Middle East: A Scoping Review And Policy Analysis, Shiva Raj Mishra, Saruna Ghimire, Chandni Joshi, Bishal Gyawali, Archana Shrestha, Dinesh Neupane, Sudesh Raj Sharma, Yashashwi Pokharel, Salim S. Virani

School of Public Health Faculty Research

This paper aims to explore the burgeoning burden of cardiovascular and metabolic disease (CMD) risk factors among South Asian labor migrants to the Middle East. We conducted a qualitative synthesis of literature using PubMed/Medline and grey literature searches, supplemented by a policy review of policies from the South Asian countries. We found a high burden of cardio-metabolic risk factors among the migrants as well as among the populations in the home and the host countries. For example, two studies reported the prevalence of diabetes mellitus (DM) ranging between 9 and 17% among South Asian migrants. Overweight and obesity were highly …

Correlation Between Pluripotent Stem Cell Markers And Microrna Expression In Dental Pulp Stem Cells, Mark Whiting

UNLV Theses, Dissertations, Professional Papers, and Capstones

In human development there are many factors that cause our cells to form certain organs and systems with specific functions. These cells original start as stem cells before biological processes take over and cause differentiation into different cell lines. Since these undifferentiated stem cells have been discovered, many studies have been done to try and find the exact mechanism that causes a cell to change into another specific cell. Due to the multiple sources that stem cells can be harvest from the human body, this knowledge of how differentiation works could lead to breakthroughs in regenerative medicine.

Traditional biomarkers have …

Analysis Of Spheno-Occipital Synchondrosis (Sos) Fusion In A Contemporary Southern Nevada Subadult Hispanic Population Using Archival Cone-Beam Computerized Tomography (Cbct) Images, Megan Baker

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study employs the three-dimensional visualization capability of cone-beam computed tomography (CBCT) to investigate the relationship between chronological age and timing of fusion of the spheno-occipital synchondrosis in a male/female subadult Hispanic population of Southern Nevada. The sample includes cross-sectional data of 374 orthodontic patients (166 males and 208 females) aged 8-20 years. The SOS is scored by a four-stage scoring system as completely open (stage 0), less than half fused (stage 1), more than half fused (stage 2), or completely fused (stage 3) as visualized in the midsagittal plane in the CBCT image. The relationship between SOS fusion stage …

Triglyceride Metabolism Following Oral Fat Tolerance Tests Of Varying Fat Content In Adults, Nathaniel Glen Bodell

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Investigations of lipid metabolism commonly utilize an oral fat tolerance test (OFTT) to elicit a dramatic increase in serum triglycerides (TG). Although this is common practice there does not currently exist an agreed upon formula for an OFTT. Nor has there been in investigation as to the reliability among commonly used OFTT. Furthermore, the effects of sex and activity level are mixed among the literature. The purpose of this investigation is to discover an ideal OFTT for research and clinical purposes, evaluate the effects of activity and sex on TG metabolism, and to test each OFTT concentration for reliability. …

The Effects Of Vascular Endothelial Growth Factor On The Characteristics Of Pluripotent Dental Pulp Stem Cells, Cale Forgues

UNLV Theses, Dissertations, Professional Papers, and Capstones

Many believe that stem cells hold the key to regenerative medicine. Therefore, research with stem cells has become increasingly popular over the years. Three specific groups of study have become present in literature. The first group has focused much of their research on harvesting and cultivating stem cell lines to retain their pluripotent ability. Stem cells can be tricky to cultivate and preserve over time as they will often differentiate or die. Another group examines the specific characteristics that a pluripotent stem cell has and how to retain, or even create, a cell with pluripotent potential. Lastly, the third group …

Optimizing Back Squat Performance, Arthur Hockwald

UNLV Theses, Dissertations, Professional Papers, and Capstones

The back squat is a task commonly used to train and test performance levels in competitive sports and strength based performance events. The purpose of this study was to analyze the potential performance benefits for a 1-repetition max (1RM) back squat under conditions in which the three key factors of OPTIMAL theory of motor learning (Wulf & Lewthwaite, 2016) are present: Enhanced expectancies (EE), autonomy support (AS), and an external focus (EF) of attention. Participants (N = 23) were assigned to either an optimized condition, which included EE AS, and EF, or a control condition. They were asked to perform …

College Student Depression: An Examination Of Cardiorespiratory Fitness, Gender And Sexual Orientation Diversity, And Help-Seeking Willingness, Sharon Jalene

UNLV Theses, Dissertations, Professional Papers, and Capstones

Depression is a serious illness characterized by persistent low mood, reduced cognitive capacity, and fatigue. Although treatable, depression is the leading cause of disability and ill-health worldwide and a significant contributor to suicide, the second leading cause of death for young Americans. In any given two-week period, 8.1% of adults in the United States had moderate to severe depression (2013-2016). The rate of depression for females was twice that of males and compared to the majority, sexual and gender minorities (SGM) were at a threefold risk. Furthermore, evidence suggests that depression incidence is three times higher in college students than …

Workload Position Evaluation To Determine Efficient Training Load For Optimal Competition Performance Of Female Collegiate Soccer Players, Michaela Kelsey

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction. The purpose of this study was to describe player workload between competition and training of female collegiate soccer players; as well as to determine workload between positions to better our understanding of the mechanical and physical demands of a full soccer match of collegiate female soccer players. Methods. The study sample was comprised of 20 female Division 1 Collegiate soccer players in 7 positions: goalkeeper (n = 1), Attacking Midfielders (n = 2), Defensive Midfielders (n = 3), Center Backs (n = 3), Outside Backs (n = 4), Strikers (n = 2), and Wingers (n = 5). 19 training …

Effects Of Ecological Restoration Techniques In Glen Canyon National Recreation Area, Simone Ka-Voka Jackson

UNLV Theses, Dissertations, Professional Papers, and Capstones

A better understanding of key ecological restoration techniques can inform land management in the Southwest on restoration options for areas infested by invasive grasses that can pose threats to ecosystems, from changes in nutrient cycling to altered fire regimes. In the semi-arid desert of Glen Canyon National Recreation Area (GLCA), several exotic grasses pose risks to local ecosystems: Saccharum ravennae, a relatively new invasive perennial grass, and Bromus rubens and Bromus tectorum, widespread annual grasses. In this study, multiple ecological restoration techniques were implemented to assess their effects on native and nonnative vegetation on sites invaded by the non-native grasses …

From Deviant Symbol To Cultural Icon? Understanding Pit Bull Stigma, Genevieve Minter

UNLV Theses, Dissertations, Professional Papers, and Capstones

This research draws from twelve months of ethnographic data using Las Vegas as a case study to understand how pit bull owners experience and negotiate stigma in various social settings. The human-dog relationship, long rooted in utility, transformed in the modern era as animals were promoted from worker to companion. The world’s first dog register, The American Kennel Club, created breed standards and encouraged selective breeding, which influenced the idea of certain dogs being more physically “dangerous” than others. Though the pit bull is not the first dog “breed” to be the object of discrimination, it is the first dog …

Effect Of Common Oxidative Water Treatments On Acanthamoeba Internalized Legionella, James Park

UNLV Theses, Dissertations, Professional Papers, and Capstones

Legionella pneumophila can cause pneumonic and non-pneumonic disease in humans. Infections occur from aerosolized contaminated water. This bacterium is an opportunistic intracellular pathogen able to infect both protozoans, such as Acanthamoeba polyphaga, and human macrophages. Both L. pneumophila and A. polyphaga resist commonly used water treatments, such as chlorination, but L. pneumophila has displayed greater resistance in the presence of A. polyphaga. Therefore, there is concern that L. pneumophila could become established in plumbing systems after water treatment, leading to infections. The objective of this study was to show the effect of chlorine and chlorine dioxide exposures on the survival …

Assessing Minimal-Input Restoration Strategies For Desert Soil And Vegetation Restoration, Audrey Jean Rader

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Mojave and Sonoran Deserts have been negatively impacted by anthropogenic disturbances. Considering that these ecosystems may recover on millennial timescales, research has shown that restoration techniques can be fairly successful in initiating long-term recovery processes in these sensitive environments. However, uncertainty remains as to which techniques are effective in different circumstances, such as in different climates or across different soil properties, and which techniques may best avoid unintended consequences, such as facilitating non-native plants. To reduce fugitive dust as a human health hazard, increase soil stability, and enhance wildlife habitat, further work is necessary to develop restoration techniques for …

Long-Term Monitoring Of Rare Gypsum Communities In The Eastern Mojave Desert: A Study In Science And Art, Tiffany Jane-Marie Pereira

UNLV Theses, Dissertations, Professional Papers, and Capstones

For land managers, reliable estimates of soil seed banks, and more specifically, change in seed banks over time, provide insight into the restoration and regeneration potential of the landscape, site history and disturbance mapping, and, invasive and rare plant management. To understand the intricacies of desert soil seed banks in relation to aboveground vegetation change, my research evaluated long-term change in soil seed banks, fertile islands, and plant communities of conservation-priority gypsum rare plant habitat of the eastern Mojave Desert. Research found significant spatial and temporal differences in seed bank composition and density over an 11-year period; however, the gypsum …

Delineation Of Events In Centripetal Migration During Drosophila Oogenesis, Travis Tait Parsons

UNLV Theses, Dissertations, Professional Papers, and Capstones

All multicellular organisms initially start out as a single cell. This cell must use the genetic information encoded in its DNA to multiply in number and build itself into a complex multicellular organism. How this process occurs is the focus of developmental biology, a field that seeks to understand how a combination of genetic information and environmental conditions shape a cell from its beginnings as a zygote all the way to maturity. A fundamental part of this process is the ability of cells to work together in order to build complex tissues and organs. Cells achieve this coordination by using …

Effects Of Disturbance And Restoration Treatment On Fertile Islands In Lake Mead National Recreation Area, Camille Traylor

UNLV Theses, Dissertations, Professional Papers, and Capstones

Disturbances by humans are one of the main drivers of change in contemporary desert ecosystems. Restoration treatments such as topsoil application and outplanting can be implemented in response to disturbances in order to maintain soil stability and a diverse plant community. Fertile islands - nutrient enriched areas beneath perennial shrubs - are fundamental features of deserts that can facilitate annual plant growth. A major uncertainty in desert ecology is how much time is required for fertile islands and nurse plant effects (the facilitation of one plant by another) to develop below maturing perennial plants. By studying naturally recruited perennial plants …