Life Sciences Commons^™

Evaluating Species Responses To Climate Change Using Ecological Niche Modeling And Genetic Data, Jeanette Perry

Undergraduate Research Opportunities Program (UROP)

The current and projected future warming trends together with degradation of habitats throughout much of the Great Basin and Columbian Plateau represent real threats to many species occupying these regions. If we can determine the impacts of past climatic changes on the distribution of species, we can obtain a better understanding of the future impacts of projected climatic trends on many species in these regions. My results with the Great Basin Pocket Mouse (Perognathus parvus) may be relevant to conservation ecologists and resource managers attempting to protect several Endangered Species Act candidates, such as the pygmy cottontail (Brachylagus idahoensis). I …

Regulation Of The Shigella Flexneri Icsp Gene And H-Ns Dependent Repression, Rosa Ojeda, Amanda Wigley, Dustin Harrison, Helen Wing

Undergraduate Research Opportunities Program (UROP)

The gram negative bacterium Shigella flexneri is known to cause dysentery in humans and primates. In order to help prevent the spread of shigellosis, gene regulation must be understood. Studies show that the virulence genes in S .flexneri are thermo regulated. At 30°C histone-like nucleoid structuring protein (H-NS) represses transcription of virulence genes and at 37°C VirB derepresses virulence genes. One of the genes that contribute to the virulence of S. flexneri is icsP. My project focuses on the regulation of the S. flexneri icsP gene and has two main goals. The first is to identify the sequence of H-NS …

Undergraduate Research Opportunities Program 2011, Nicholle Booker, University Of Nevada, Las Vegas

Undergraduate Research Opportunities Program (UROP)

Undergraduate Research Opportunities Program

On August 9, 2011 the UNLV College of Sciences will celebrate the accomplishments of undergraduate students participating in the Summer 2011 Undergraduate Research Opportunities Program (UROP) and the Research Experience For Undergraduates (REU) Program.

The public is invited to attend, beginning at 10:00 a.m.

Please join us to view student research posters. Student research topics include: biomedicine and human health, Nevada's fragile environment and ecosystems, climate change, stem cell research, microbiology, astrophysics, and many others.

Over 25 UNLV undergraduates and a cohort of 25 undergraduates selected from colleges and universities across the nation will mark the …

Synthesis Of A Chemical Probe For Identification Of Ger Receptor Proteins Of Bacillus Anthracis, Hyelee Lee

UNLV Theses, Dissertations, Professional Papers, and Capstones

Bacillus anthracis,a gram positive, endospore-forming, rod-shaped, aerobic bacterium, is the causative agent of anthrax. A key step in the pathogenic cycle is the spore germination, where the dormant spore transforms into a metabolically active cell. This allows B. anthracis to proliferate and secrete toxins. It is believed that spore germination is triggered when small molecules, known as germinants, are recognized by germination (Ger) receptor proteins located in the inner membrane of spores. Most Ger proteins are encoded by tricistronic operons, resulting in three distinct products, the A-, B-, C-subunits. Genomic sequencing showed that B. anthracis has seven ger receptor …

Climate And Vegetation Change In The Newberry Mountains, Southern Clark County, Nevada, Ross Joseph Guida

UNLV Theses, Dissertations, Professional Papers, and Capstones

Ecological studies have shown worldwide that vegetation is being affected by climate change. Species are shifting to new elevations and physiographic positions to adapt to changes in their environment. More specifically, paleoecology studies in the Mojave Desert have shown shifting vegetation patterns in response to past warming and precipitation changes. Recent studies have shown mortality among desert plants related to extended drought and warming. However, few studies have shown how the geographic distribution of Mojave Desert species has changed during this most recent period of warming. This study addresses this gap in the literature by focusing on several plant species …

Embryonic Development Of The Grass Shrimp, Palaemonetes Pugio, And The Influence Of Salinity On Cardiac Physiology, Amie Lynn Romney

UNLV Theses, Dissertations, Professional Papers, and Capstones

The grass shrimp, Palaemonetes pugio, inhabit the brackish waters along the Atlantic coast of North America and are a key component of the estuarine ecosystem. These decapod shrimp can tolerate large fluctuations in environmental parameters including daily and seasonal fluctuations in salinity between 0 to 55 parts per thousand (seawater at 30-32 ppt). Any observed distribution patterns of adult P. pugio in relation to salinity may be accounted for by their relative ability to tolerate a range in salinity and their ability to maintain internal water volumes and cardiac performance in the earliest life stage, the embryonic period. This thesis …

Historical Diversification Of Montane Herpetofauna Within And Between The Sierras Of Mexico, Robert William Bryson Jr.

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Mexican highlands consist of four major mountain ranges spanning most of mainland Mexico. The evolutionary history of the Mexican highlands has been shaped by various geological and climatic events over the past several million years. The relative impacts of these historical events on diversification in montane taxa, however, remains uncertain. I used mitochondrial DNA data from three widely distributed species complexes of lizards as a model system to exemplify the potential roles of Neogene mountain formation and Quaternary climate change on timing and tempo of diversification across the Mexican highlands. My results suggested strong geographic partitioning of genetic variation …

Phylogeography Of A Vanishing North American Songbird: The Painted Bunting (Passerina Ciris), Connie Ann Herr

UNLV Theses, Dissertations, Professional Papers, and Capstones

Studies of genetic variation within and between species can provide insights into their evolutionary history as well as important information for conserving biodiversity. An understanding of population processes can assist in the conservation of biodiversity by contrasting current versus historical patterns, and the processes that have generated these patterns. Genetic differentiation often coincides with significant geological or climatic changes that have shaped the sizes and locations of the species geographic range and altered the connectivity between populations over time. Phylogenetic and population genetic analyses can also provide a statistical framework for the investigation of how human processes such as habitat …

Walking Box Ranch Planning And Design Quarterly Progress Report: Period Ending July 10, 2010, Margaret N. Rees

Walking Box Ranch

• UNLV participated in several meetings with BLM regarding updating the current Task Order, obtaining a two-year no-cost extension for the two Assistance Agreements, and to develop the future WBR operating agreement between BLM and UNLV
• UNLV met with Sara Moffatt, one of Senator Reid’s staff, to provide an update on project progress. UNLV also requested the Senator’s support in obtaining additional funding for the project for construction, and also for UNLV to bridge the transition from construction through the first two years of operation.
• UNLV held several internal meetings to advance planning for a July VA meeting with BLM to …

How Well Do U.S. Forest Service Terrestrial Ecosystem Surveys Correspond With Measured Vegetation Properties?, Scott R. Abella

Life Sciences Faculty Research

No abstract provided.

Mojave Applied Ecology Notes Summer 2011, Scott R. Abella, Charles W. Denton, David G. Brewer, Rory W. Steinke, Wayne A. Robbie, W. Wallace Covington, E. Cayenne Engel, Ross Guida

Mojave Applied Ecology Notes

Thoughts on fire possibilities on Mount Charleston, estimating historical densities of Ponderosa pine in northern AZ, over-understory changes in the Spring Mountains, seeding effectiveness in Red Rock Canyon, workshop announcements.

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending June 30, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 42 juvenile/adult samples and analyses of the remaining samples are underway.
• As of 7/07/11 all sample sets will have been collected for Veliger abundance and settlement at different depths of Lake Mead; sampling continues for Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay.
• The Interagency Quagga Mussel Meeting was held on 05/17/11; the next meeting is scheduled for 08/25/11.

Project 2

• Appendix 7, an addition to the Long-term Limnological and Aquatic …

Botteri's Sparrow (Peucaea Botterii) Occurs In Northern Coahuila, Mexico, Paul Van Els, Ricardo Canales-Del-Castillo, John Klicka

Ornithology Program (HRC)

Botteri’s Sparrow (Peucaea botterii) occurs widely in the shrub-grasslands of southern North America. We report a breeding population of the species in the Sierra de la Encantada of northern Coahuila, Mexico, ~80 km from the Big Bend area of Texas and >300 km from the nearest previously known breeding range in southern Coahuila and central Chihuahua. We captured three individuals, which show a mostly gray dorsal coloration, suggestive of the texana subspecies, occurring from southern Texas to northern Veracruz. The exact affinity of the northern Coahuila population still needs to be ascertained. The presence of Botteri’s Sparrow in northern Coahuila …

Characterization And Regulation Of The Icsp And Ospz Locus In Shigella Flexneri, Krystle Lashell Pew

UNLV Theses, Dissertations, Professional Papers, and Capstones

Shigella flexneri is a gram negative, rod shaped bacterium that is the causative agent of bacillary dysentery, which is characterized by fever, abdominal pain, and bloody diarrhea. Genes essential to the pathogenicity of S. flexneri are encoded by a virulence plasmid. Shigella has evolved a complex regulatory system to regulate transcription of virulence genes. This involves two regulators, VirF and VirB, which allow the bacterium to respond to environmental stimuli and maximally exploit host niches. An additional factor impacting virulence gene regulation is H-NS, a histone nucleoid structuring protein that globally represses transcription. This work addresses the transcriptional regulation of …

Hivtoolbox, An Integrated Web Application For Investigating Hiv, David P. Sargeant, Sandeep Deverasetty, Yang Luo, Angel Villahoz Baleta, Stephanie Zobrist, Viraj Rathnayake, Jacqueline C. Russo, Jay Vyas, Mark A. Muesing, Martin Schiller

Life Sciences Faculty Research

Many bioinformatic databases and applications focus on a limited domain of knowledge federating links to information in other databases. This segregated data structure likely limits our ability to investigate and understand complex biological systems. To facilitate research, therefore, we have built HIVToolbox, which integrates much of the knowledge about HIV proteins and allows virologists and structural biologists to access sequence, structure, and functional relationships in an intuitive web application. HIV-1 integrase protein was used as a case study to show the utility of this application. We show how data integration facilitates identification of new questions and hypotheses much more rapid …

The Role Of Historical And Contemporary Processes On Phylogeographic Structure And Genetic Diversity In The Northern Cardinal, Cardinalis Cardinalis, Brian T. Smith, Patricia Escalante, Blanca E. Hernandez-Banos, Adolfo G. Navarro-Siguenza, Sievert Rohwer, John Klicka

Ornithology Program (HRC)

Background

Earth history events such as climate change are believed to have played a major role in shaping patterns of genetic structure and diversity in species. However, there is a lag between the time of historical events and the collection of present-day samples that are used to infer contemporary population structure. During this lag phase contemporary processes such as dispersal or non-random mating can erase or reinforce population differences generated by historical events. In this study we evaluate the role of both historical and contemporary processes on the phylogeography of a widespread North American songbird, the Northern Cardinal, Cardinalis cardinalis …

Estimating Wildfire Potential On A Mojave Desert Landscape Using Remote Sensing And Field Sampling, Peter F. Van Linn Iii

UNLV Theses, Dissertations, Professional Papers, and Capstones

Landscape level wildfire prediction can be used to allocate wildfire resources and guide land management practices. Wildfire prediction in arid habitats in the Southwestern United States is of specific concern because of the negative ecological impacts of fire on desert habitats and the current lack of accurate fire prediction tools for such areas. This study examines the ability to predict previous fire occurrences and estimate future fire potential using satellite imagery and on the ground field survey techniques along with ignition potential data (lightning strikes and distance to roads), topographical data (elevation and aspect), and climate information (maximum and minimum …

Relationships Of Exotic Plant Invasions With Biological Soil Crust, Desert Pavement, And Soil Carbon In The Eastern Mojave Desert, Adria Decorte

UNLV Theses, Dissertations, Professional Papers, and Capstones

In a matter of 50 years, exotic annual plants have become widespread in the Mojave Desert, contributing to drastic landscape changes such as those caused by recent fires. Invasions by exotics threaten native Mojave Desert plant communities by altering community functions (e.g. fire regimes) and by reducing plant diversity. Because it is not practical, or even possible, to eradicate these exotics, developing effective prevention techniques is the key to controlling these invasions.

This thesis used a greenhouse experiment, a field experiment, and a correlational field study to examine the affect soil surface types have on the establishment of three exotic …

The Acute Effects Of Various Types Of Stretching (Static, Dynamic, Ballistic, And No Stretch) Of The Iliopsoas On 40-Yard Sprint Times In Non-Athletes, Scott David Christensen, Craig Perry, Ryan Richard Resnik

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to determine the effect of static, ballistic, dynamic, and no stretching immediately prior to a 40-yard sprint in college students. There were 35 healthy subjects (22 male and 13 female) between the ages of 24 and 37 (Mean = 26.46 yrs, SD = 2.99 yrs) who participated. The experiment consisted of running 4, 40-yard sprint trials immediately following 1 of 4 different stretching protocols. Prior to each 40- yard sprint trial, a 5-minute warm up was performed at 3.5 mph on a treadmill. Each subject received each of the four techniques in a randomized …

Examining Activity Levels And Motor Proficiency: A Comparison Of Children Who Are Overweight And At A Healthy Weight To Their Parents And Peers, Aaron Copeland, Maresa Madsen, Riley Phelps, Brandon Richards

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objective: The purpose of this study was to examine relationships and differences between motor proficiency, activity level, and parental activity level in children who are at a healthy weight and children who are overweight or obese.

Methods: Forty-four children (26 children at a healthy weight and 18 children who were overweight or obese) between the ages of 8-16 (BMI: 14.3-43.6 kg/m2) and 36 parents (BMI: 18.1-44.7) participated in this study. Children and parents wore StepWatch activity monitors (SAM) to measure activity levels over a 72 hour period. Tests reflecting several determinants of motor performance were also administered to …

The Acute Effects Of Static Stretching Of The Gastrocnemius On Limits Of Stability In Young Adults Versus Elderly Adults, Matt Bugnet, Kirk Player

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background and Purpose: Balance is an important part of everyday life for all individuals with many body systems interacting to achieve optimal balance. Proprioceptive organs aid in this interaction, while also acting as stretch receptors. Therefore, it is possible that stretching may influence the overall balance of the individual. The purpose of this study was to investigate the acute effects of stretching on dynamic balance of healthy young and elderly adults. The two groups were used to determine if stretching may discriminately affect balance at different ages.

Subjects: Thirty healthy adults between the ages of 18 and 35 …

Effects Of Environment On Children's Motor Scores, Eligibility Status, And Administration Times, Derrick Mittelstadt, Abigail Parker, Kirsten Pickett, Heather Temkin

UNLV Theses, Dissertations, Professional Papers, and Capstones

Best practices for assessing developmental skills in young children focus on naturalistic observation in everyday settings, but the effects of environment on test scores, eligibility status and administration time have not been explored. The Peabody Developmental Motor Scales-Second Edition (PDMS-2) was administered to 34 children aged 18 to 59 months in natural and pull-out settings. PDMS-2 total, gross, and fine motor quotient (TMQ, GMQ, and FMQ) scores were significantly lower in the natural environment (p’s≤.014). Based on our results, more children would qualify for services when tested in natural environments using TMQ and GMQ scores. It also took significantly longer …

The Self: Your Own Worst Enemy? A Test Of The Self-Invoking Trigger Hypothesis, Bradley J. Mckay

UNLV Theses, Dissertations, Professional Papers, and Capstones

The self invoking trigger hypothesis was recently proposed by Wulf and Lewthwaite (2010) as the mechanism underlying the robust effects of attentional focus on motor learning and performance. The hypothesis suggests that causing individuals to access their self schema will negatively impact their ability to learn and perform a motor skill. The purpose of the present study was to provide an initial test of this hypothesis by causing one group of participants to activate their self schema in a straightforward manner. Participants (N = 32) were assigned to either a self-activated or control condition and asked to practice a wiffleball …

Molecular Evolution And Historical Biogeography Of New World Birds, Brian T. Smith

UNLV Theses, Dissertations, Professional Papers, and Capstones

Deciphering the patterns of how biodiversity has evolved across time and space has remained a fundamental objective for biologists for the last 200 years. Researchers are faced with the challenge of interpreting the complexity of evolutionary patterns that have been generated over the deep history of the Earth. The advancement of DNA sequencing technology has yielded a new and powerful genetic toolkit that has allowed biologists to address novel evolutionary questions. For my dissertation research, I used molecular genetics and a statistical framework to study the evolution and historical biogeography of birds distributed in North and South America. My dissertation …

Second-Order Conditioning In Drosophila, Christopher J. Tabone

UNLV Theses, Dissertations, Professional Papers, and Capstones

Animals possess the ability to associate neutral stimuli in their environment with both rewards and punishment. A conditioned stimulus (CS1) such as a smell or sound, can become associated with an unconditioned stimulus (US), such as a food reward, to elicit what is known as the conditioned response (CR). This type of learning is commonly referred to as classical conditioning or first-order conditioning (FOC). Second-order conditioning (SOC) is an extension of this type of association wherein a novel stimulus is introduced (CS2) and associated with a previously conditioning first-order stimulus (CS1). As a result, the organism may show an attraction …

Planning For Building-Integrated Agriculture In Las Vegas, Robert Vralsted

UNLV Theses, Dissertations, Professional Papers, and Capstones

High food prices, concern about food nutrition and safety, and an awareness of commercial farming's environmental impact have generated a renewed interest in sustainable urban agriculture. Advances in controlled environment agriculture (CEA) have made it possible to grow food virtually anywhere in a much more sustainable manner than traditional field-based agriculture. Locating and planning urban farms using retrofitted existing building stock to maximize food production and ease of distribution in Las Vegas requires consideration of multiple barriers related to geography, economics, and the built environment. Consideration of these factors in the planning process informs the design of a successful BIA …

Phylogeography Of Three Heteromyid Taxa: Insight On The Evolution Of A North American Arid Grassland Rodent Guild, Sean A. Neiswenter

UNLV Theses, Dissertations, Professional Papers, and Capstones

Revealing how communities are shaped by abiotic and biotic factors plays a central role in biogeographic and comparative phylogeographic studies. The biogeography of North American arid grasslands is explored using nuclear and mitochondrial DNA from three groups of heteromyid rodents that are broadly sympatric in aridlands across western North America. Phylogenetic and molecular clock analyses are used to estimate the timing of divergences within each group. A general pattern of late Miocene divergence and expansion of lineages in each of the groups that is coincident with the rapid expansion of arid grasslands at the time. The initial divergence is followed …

An Ecological Study Of Peregrine Falcons (Falco Peregrinus) At Lake Mead National Recreation Area, 2006-2010, Joseph Graham Barnes

UNLV Theses, Dissertations, Professional Papers, and Capstones

Peregrine Falcons (Falco peregrinus) represent an encouraging conservation biology success story in North America during the twentieth century. Their distribution and population size suffered major restrictions after the initiation of widespread application of the synthetic pesticide dichloro-diphenyl-trichloroethane (DDT) within the U.S. in the 1940s. The species was federally listed as endangered in the U.S. in 1969 and was then delisted in 1999 after DDT was banned in 1972. Herein, I present my ecological research of peregrines within Lake Mead National Recreation Area (LMNRA), concentrating on the years 2006-2010. This thesis is comprised of two chapters. In the first chapter, I …

The Effect Of Acute Exercise-Induced Fatigue On Pistol Shooting Performance In Police Officers, Melissa Jean Brown

UNLV Theses, Dissertations, Professional Papers, and Capstones

Duties of police officers include apprehending suspects, which may involve foot pursuit and firing a weapon at a suspect. Previous studies in biathletes and soldiers suggest that shooting performance is compromised when fatigue is present. Purpose: To investigate the effects of exercise-induced fatigue on shooting performance in police officers. Methods: Nine police officers fired five rapid-fire shots at three targets each with a fifteen second rest period in between each trail. Officers then exercised on a cycle ergometer to 85% maximum heart rate or voluntary fatigue. The pretest shooting was repeated post-exercise. Distances of 4 shots from the center of …

The Effect Of Self-Controlled Practice On Forearm Passing, Motivation, And Affect In Women’S Volleyball Players, Nels Rydberg

UNLV Theses, Dissertations, Professional Papers, and Capstones

Motor learning research has suggested that self-controlled practice (or “autonomy”) leads to more effective learning of motor tasks. Debate continues, however, as to why. Most motor behaviorists maintain the better learning is due to cognitive and information-processing factors. Recently, others have proposed the learning enhancement is due to such psychological factors as motivation and affect. The present study sought to measure motor skill learning, intrinsic motivation, and affect in self-controlled versus externally-controlled (yoked) practice conditions.

Participants, 16 collegiate women’s volleyball student-athletes from two National Collegiate Athletic Association Division I programs, were paired by forearm passing skill level, and one of …