Life Sciences Commons^™

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 3, Edition 3, March 2024, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

K-12 curriculum and resources

CASNR Ag Tech Day, April 23

2024 Summer Soybean Institute, May 29-30 and July 16-18

Youth Energy Leadership Camp July 22-26

Other upcoming events

Analytic investigators spotlight: UNL Statstics, Forensic Science, Ag Economics

Educators workshops

Student opportunities including NAYI, Native American Coalition, Cultivando Caminos, Big Red Camps, and more

Teacher opportunities including Economic tidbits from …

Field Research Report: Results From The Enreec Vri Field For The 2021, 2022, And 2023 Crop Seasons, Derek M. Heeren, Ali T. Mohammed, Eric Wilkening, Christopher M. U. Neale, Alan L. Boldt, Ankit Chandra, Precious Nneka Amori, Ivo Z. Goncalves, Yeyin Shi, Guillermo R. Balboa

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Long-term irrigation management research has been conducted from 2014 to 2023 for corn and soybean at the Eastern Nebraska Research, Extension, and Education Center (ENREEC) Variable Rate Irrigation (VRI) Field located in subhumid east-central Nebraska (in the Lower Platte North Natural Resources District). The objective of this report was to present the overall results from the VRI Field for 2021 to 2023. Across the three growing seasons, there were the following irrigation treatments: Best Management Practice (BMP), 50% BMP, 125% BMP, rainfed, Spatial ET Modeling Interface (SETMI), SDD1, SDD2, machine-learning-based Cyber-Physical System (CPS), a student team recommended rate, and industry …

Nebraska Ornithologists' Union Treasurer's Report, December 31, 2023, Jan Johnson

Nebraska Bird Review

No abstract provided.

Synopsis Of The Genus Cerabilia, André Larochelle, Marie-Claude Larivière

Insecta Mundi

The genus Cerabilia Laporte de Castelnau (Carabidae: Abacetini) is revised for New Zealand. Thirteen species are recognized.

Seven species are described as new: Cerabilia (Cerabilia) cordata Larochelle and Larivière new species, Cerabilia (Cerabilia) kaihoka Larochelle and Larivière new species, Cerabilia (Cerabilia) laevis Larochelle and Larivière new species, Cerabilia (Cerabilia) motunau Larochelle and Larivière new species, Cerabilia (Cerabilia) rugosa Larochelle and Larivière new species, Cerabilia (Cerabilia) takaka Larochelle and Larivière new species, Cerabilia (Cerabilia) willi Larochelle and Larivière new species. …

Birds And The People They Were Named For

Nebraska Bird Review

This year the theme for our color photo section was birds named for people. For the purposes of our selections, we looked only at the common names of birds, although some species also reflect the names of people in their scientific names. Many species are named for people, but photos of the ones listed here are on the first six pages of this year’s color section.

Baird’s Sandpiper, Baird’s Sparrow Spencer Fullerton Baird (1823-1887) was the first curator and later secretary of the Smithsonian Institution and was instrumental in expanding the Institution’s natural history collections. John James Audubon named the …

Winter Field Report, December 2023–February 2024, W. Ross Silcock

Nebraska Bird Review

As was the case for winter 2022–2023, the big general story was the large number of reports of tardy fall migrants and unexpectedly northerly occurrences in midwinter, an apparent residue of relatively mild early winter temperatures. These reports involved at least 50 species, many of them waterbirds and native sparrows, but including several other passerines as well. Notable were about 12 waterfowl species, five grebe species, an interesting late Dec peak in each of the last three years of Sandhill Cranes, record late Whooping Cranes, a 4th winter record of Dunlin, high numbers of Double-crested Cormorants, a wintering Rock Wren, …

Nebline, March/April 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Seven Garden Gaffes to Avoid This Year by Sarah Browning

Youth Advance Health Equity with Community-Based Research by Emily Gratopp

Recipe of the Month: Crunchy Kohlrabi Salad by Emily Gratopp

GROBigRed Virtual Learning Series

Garden Guide: Things to Do This Month

2024 Lincoln Early Childhood Conference

2024 Chemigation Training

Corn Yield Challenge for 4-H and FFA Members

Lancaster County Ag VIP Awards

4-H Teens Work Together to Lead the Lock-In

4-H Horse/Livestock Judging Teams at Nationals

Heart of 4-H Volunteer Award: Sheridan Swotek and Kim Bowen

4-H Club Gives Youth With Disabilities Opportunity to Show Lambs

4-H Rabbit Show, March …

Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas

Department of Entomology: Dissertations, Theses, and Student Research

Pollinators are intrinsically linked to the success of unmanaged and managed ecosystems by providing pollination services that aid in the reproduction of wildflowers and many crops. Land use change, habitat loss, fragmentation, and related landscape-level phenomena (for example, increased pesticide exposure) threaten pollinators and have been associated with population declines. In the Midwestern region of the United States, land conversion of native prairies and grasslands to row-crop agriculture has been one of the largest contributors to pollinator habitat loss. Conservation programs, such as the Conservation Reserve Program, have worked towards removing environmentally sensitive lands from agriculture production and enrolling them …

A Dietary Variety: Analyzing The Impacts Of Diverse Vs Worm-Exclusive Diets On The Blanding’S Turtle (Emydoidea Blandingii), Caitlin F. Copenhaver

Honors Program: Senior Projects (Public)

The endangered Blanding’s turtle (Emydoidea blandingii) is a species with a generally diverse diet in their freshwater wetland habitats; they consume prey such as crayfish and snails. In captivity, these options often become more limited due to logistical reasons. As ex situ conservation efforts such as captive rearing and head-starting are becoming more popular for this species, it will be important to gain more insight on how these more limited diets might affect these turtles. This study describes a comparison between diets in six captive Blanding’s turtles from September to November of 2023, with three turtles receiving only …

Evaluation Of Ndot’S Sediment Barrier Practices Using Performance Data, Michael A. Perez, Wesley N. Donald, J. Blake Whitman, Brian G. Roche

Nebraska Department of Transportation: Research Reports

To protect waterways adjacent to construction projects with disturbed land, a 50 ft (15 m) vegetated buffer or equivalent sediment controls are required. However, there is little guidance on the effectiveness of vegetated buffers in removing sediment or how sediment barriers can aid shorter buffers or replace buffers. A modeling methodology was developed and used to determine the performance of 11,664 50 ft (15 m) vegetated buffer configurations with Nebraska conditions; sediment capture averaged 92.6% and ranged from 18.5% to 99.5%. To determine the performance of Nebraska Department of Transportation standard and modified sediment barrier installations, a large-scale testing methodology …

Soil Nitrate Prediction Using Ftir-Atr, Vis-Nir, And Raman Spectroscopy, Sabiha Ferdous

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Fourier transform infrared spectroscopy (FTIR) combined with Attenuated total reflectance (ATR), Visible-Near Infrared spectroscopy (Vis-NIR), and Raman spectroscopy (RS) are non-destructive techniques for rapid determination of nitrogen compounds in soil. Leveraging FTIR-ATR and Vis-NIR spectra using partial least squares regression (PLSR) modeling, the study aims to predict soil nitrate content and explored the feasibility of Raman spectroscopy to detect nitrate (NO₃^-), nitrite (NO₂^-), and ammonium (NH₄⁺) in soil. Soil samples were collected from four different fields, dried, sieved (2mm), and then used for collecting spectra (FTIR-ATR and Vis-NIR). Laboratory analysis was …

Anthrenus (Anthrenus) Muehlei, A New Species (Coleoptera: Dermestidae: Megatominae) From Iran, Graham J. Holloway, Andreas Herrmann

Insecta Mundi

A new species, Anthrenus muehlei Holloway and Herrmann (Coleoptera: Dermestidae: Megatominae), from Iran is described. Images of internal and external features are presented. Only female specimens were found and described, but the bursa copulatrix contains obvious sclerites enabling easy differentiation from all other known species from the Palaearctic A. pimpinellae complex. The possible function and taxonomic implication of the sclerites is mentioned.

ZooBank registration. urn:lsid:zoobank.org:pub:97661C20-803E-4785-A4B0-58B16C94D9A2

New Host Records For Nearctic And Neotropical Spider Wasps (Hymenoptera: Pompilidae), Frank E. Kurczewski, Rick C. West, Cecilia Waichert

Insecta Mundi

We present new and atypical host records for 78 species, subspecies, species-groups, and variants of spider wasps (Hymenoptera: Pompilidae) from North America and South America. The records are listed in modified taxonomic order following the Catalog of Hymenoptera in America North of Mexico, Volume 2, Apocrita (Aculeata) (Krombein 1979). These records are an extension of previous host records reported by Kurczewski (2010), Kurczewski and Edwards (2012), and Kurczewski et al. (2017, 2020, 2022b). New genus and species host records are given for the genera and subgenera Calopompilus Ashmead, Chirodamus Haliday, Pepsis Fabricius, Hemipepsis Dahlbom, Priocnessus Banks, Entypus Dahlbom, Pompilocalus Roig-Alsina, …

Annual Color Photo Section

Nebraska Bird Review

Annual color photograph section (the first six pages show examples of this year’s theme: species whose common names include the names of people):

Cassin’s Finch
Cassin’s Kingbird
Steller’s Jay
Cooper’s Hawk
Baird’s Sandpiper
Baird’s Sparrow
Bullock’s Oriole
Hybrid Baltimore × Bullock’s Oriole
Clark’s Grebes
Sprague’s Pipit
Forster’s Tern
Brewer’s Sparrow
Nelson’s Sparrow
Swainson’s Hawk
Swainson’s Thrush
Clark’s Nutcracker
Ross’s Goose
Sabine’s Gull
LeConte’s Sparrow
Harris’s Sparrow
Henslow’s Sparrow
Townsend’s Solitaire
Wilson’s Warbler
Wilson’s Snipe
Lincoln’s Sparrow
Wilson’s Phalarope
Franklin’s Gulls
Pine Grosbeak
Bald Eagle
Pine Siskin
Piping Plover
Ovenbird
Blackheaded Grosbeak
Red Crossbill
Ash-throated Flycatcher
Long-eared Owl
Horned Grebes

The Nebraska Bird Review (March 2024) 92(1), Whole Issue

Nebraska Bird Review

Winter Field Report, December 2023–February 2024, by W. Ross Silcock

Birds and the People They Were Named For

Annual Color Photo Section

2023–2024 Christmas Bird Counts, by Don Paseka

Annual Treasurer’s Report for 2023, by Jan Johnson

Subscription and Organization Information

2023–2024 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

With the resurrection of the Kearney count, twenty CBCs [Christmas Bird Counts] were run in the 2023–2024 season. This is four more than last year and one more than the previous high number of nineteen from 2021. Although the Crawford, Harrison, and Scottsbluff counts were postponed because of snow, the weather on count days was mild with an average high of 43°F and an average low of 27°F, resulting in mostly open water. Rain and mud eliminated access to several normally productive areas of the Harlan County count which still produced the highest number of species (94). Lake McConaughy produced …

Variation In Leaf Chlorophyll Concentration In Response To Nitrogen Application Across Maize Hybrids In Contrasting Environments, Kyle M. Linders, Dipak K. Santra, James C. Schnable, Brandi Sigmon

Department of Agronomy and Horticulture: Faculty Publications

Leaf chlorophyll concentration was measured for 84 publicly available maize hybrids grown under three nitrogen fertilizer treatments in two contrasting environments in Nebraska. The effect of nitrogen treatment on chlorophyll response was found to be significant (p < 0.05) for both locations. In Scottsbluff, chlorophyll concentrations increased significantly with increasing nitrogen rate, while no significant difference was found between medium and high nitrogen in Lincoln. Within equivalent nitrogen treatments, chlorophyll was more abundant in Lincoln than Scottsbluff for nearly every hybrid. Hybrid response was not consistent between environments, with approximately 11% of variance explained by genotype by environment interaction.

Bermad: Batch Effect Removal For Single-Cell Rna-Seq Data Using A Multi-Layer Adaptation Autoencoder With Dual-Channel Framework, Xiangxin Zhan, Yanbin Yin, Han Zhang

Department of Food Science and Technology: Faculty Publications

Motivation: Removal of batch effect between multiple datasets from different experimental platforms has become an urgent problem, since single-cell RNA sequencing (scRNA-seq) techniques developed rapidly. Although there have been some methods for this problem, most of them still face the challenge of under-correction or over-correction. Specifically, handling batch effect in highly nonlinear scRNA-seq data requires a more powerful model to address under-correction. In the meantime, some previous methods focus too much on removing difference between batches, which may disturb the biological signal heterogeneity of datasets generated from different experiments, thereby leading to over-correction.

Results: In this article, we …

2022 Census Of Agriculture: Nebraska Highlights, Kathleen Brooks, Bradley D. Lubben

Cornhusker Economics

In February 2024, the United States Department of Agriculture National Agricultural Statistics Service published data from the 2022 Census of Agriculture. This data is collected every five years. The current article highlights a few of the Nebraska numbers. Two significant highlights from the data are the total number of farms and the total value of production. U.S. farm numbers declined modestly in the U.S. from 2.04 million in 2017 to 1.90 million in 2022. The total value of production for U.S. farms and ranches increased substantially from $388.5 billion in 2017 to $543.1 billion in 2022. Crop production accounted for …

Calibration Of Hybrid-Maize Model For Simulation Of Soil Moisture And Yield In Production Corn Fields, Anthony A. Amori, Olufemi P. Abimbola, Trenton E. Franz, Daran Rudnick, Javed Iqbal, Haishun Yang

Department of Agronomy and Horticulture: Faculty Publications

Model calibration is essential for acceptable model performance and applications. The Hybrid-Maize model, developed at the University of Nebraska-Lincoln, is a process-based crop simulation model that simulates maize growth as a function of crop and field management and environmental conditions. In this study, we calibrated and validated the Hybrid-Maize model using soil moisture and yield data from eight commercial production fields in two years. We used a new method for the calibration and multi-parameter optimization (MPO) based on kriging with modified criteria for selecting the parameter combinations. The soil moisture-related parameter combination (SM-PC3) improved simulations of soil water dynamics, but …

Vitamin E Biofortification: Enhancement Of Seed Tocopherol Concentrations By Altered Chlorophyll Metabolism, Ping Qin, Peng Chen, Yuanwei Zhou, Wei Zhang, Yunyun Zhang, Jingjing Xu, Lu Gan, Yingnan Liu, Jill Romer, Peter Dörmann, Edgar B. Cahoon, Chunyu Zhang

Department of Biochemistry: Faculty Publications

Homogentisate Phytyltransferase (HPT) catalyzes condensation of homogentisate (HGA) and phytyl diphosphate (PDP) to produce tocopherols, but can also synthesize tocotrienols using geranylgeranyl diphosphate (GGDP) in plants engineered for deregulated HGA synthesis. In contrast to prior tocotrienol biofortification efforts, engineering enhanced tocopherol concentrations in green oilseeds has proven more challenging due to the integral role of chlorophyll metabolism in supplying the PDP substrate. This study show that RNAi suppression of CHLSYN coupled with HPT overexpression increases tocopherol concentrations by >two-fold in Arabidopsis seeds. We obtained additional increases in seed tocopherol concentrations by engineering increased HGA production via overexpression of …

Relation Of Life Sciences Students’ Metacognitive Monitoring To Neural Activity During Biology Error Detection, Mei Grace Behrendt, Caron Clark, Mckenna Elliott, Joseph Dauer

Department of Educational Psychology: Faculty Publications

Metacognitive calibration—the capacity to accurately self-assess one’s performance—forms the basis for error detection and self-monitoring and is a potential catalyst for conceptual change. Limited brain imaging research on authentic learning tasks implicates the lateral prefrontal and anterior cingulate brain regions in expert scientific reasoning. This study aimed to determine how variation in undergraduate life sciences students’ metacognitive calibration relates to their brain activity when evaluating the accuracy of biological models. Fifty undergraduate students enrolled in an introductory life sciences course completed a biology model error detection task during fMRI. Students with higher metacognitive calibration recruited lateral prefrontal regions linked in …

The Spliceosome-Associated Protein Cwc15 Promotes Mirna Biogenesis In Arabidopsis, Bangjun Zhou, Huihui Yu, Yong Xue, Mu Li, Chi Zhang, Bin Yu

School of Biological Sciences: Faculty Publications

MicroRNAs (miRNAs) play a key role in regulating gene expression and their biogenesis is precisely controlled through modulating the activity of microprocessor. Here, we report that CWC15, a spliceosome-associated protein, acts as a positive regulator of miRNA biogenesis. CWC15 binds the promoters of genes encoding miRNAs (MIRs), promotes their activity, and increases the occupancy ofDNA-dependent RNA polymerases atMIR promoters, suggesting that CWC15 positively regulates the transcription of primary miRNA transcripts (pri-miRNAs). In addition, CWC15 interacts with Serrate (SE) and HYL1, two key components of microprocessor, and is required for efficient primiRNA processing and the HYL1-pri-miRNA interaction. Moreover, CWC15 interacts with …

African Swine Fever Virus Early Protein Pi73r Suppresses The Type-I Ifn Promoter Activities, Danh C. Lai, Jayeshbhai Chaudhari, Hiep L.X. Vu

Department of Animal Science: Faculty Publications

African swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dA:dT), a strong inducer of the cGAS-STING signaling pathway. Although the I73R protein exhibits structural homology with the Zα domain binding to the left-handed helical form of DNA known as …

Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis By Modulating The Expression Of Apoptotic Genes, Members Of Polycomb Repressive Complex 2, And Levels Of Tri-Methylating Lysine 27 At Histone 3 In Human Malignant Melanoma Cells, Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E. Giova, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis L. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were …

Application Of Dna Barcodes In The Genetic Diversity Of Hard Ticks (Acari: Ixodidae) In Kazakhstan, Ziwei Zheng, Weixin Zeng, Suwen Wang, Wenbo Tan, Xiaobo Lu, Kenesbay Kairullayev, Ligu Mi, Wurelihazi Hazihan, Gang Liu, Meihua Yang, Yuanzhi Wang

Harold W. Manter Laboratory of Parasitology: Library Materials

Forty-five tick species have been recorded in Kazakhstan. However, their genetic diversity and evolutionary relationships, particularly when compared to ticks in neighbouring countries, remain unclear. In the present study, 148 mitochondrial cytochrome c oxidase subunit I (COI) sequence data from our laboratory and NCBI (National Center for Biotechnology Information; https://www.ncbi.nlm.nih.gov/) data were used to address this knowledge gap. Phylogenetic analyses showed that i) Hyalomma anatolicum anatolicum (Koch, 1844) ticks from Jambyl Oblast (southeastern Kazakhstan) and Gansu Province (northwestern China) constituted a newly deviated clade; and ii) Dermacentor reticulatus (Fabricius, 1974) ticks from South Kazakhstan Oblast were closer …

Antibody Profiling And Predictive Modeling Discriminate Between Kaposi Sarcoma And Asymptomatic Kshv Infection, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood

School of Biological Sciences: Faculty Publications

Protein-level immunodominance patterns against Kaposi sarcoma-associated herpesvirus (KSHV), the aetiologic agent of Kaposi sarcoma (KS), have been revealed from serological probing of whole protein arrays, however, the epitopes that underlie these patterns have not been defined. We recently demonstrated the utility of phage display in high-resolution linear epitope mapping of the KSHV latency-associated nuclear antigen (LANA/ORF73). Here, a VirScan phage immunoprecipitation and sequencing approach, employing a library of 1,988 KSHV proteome-derived peptides, was used to quantify the breadth and magnitude of responses of 59 sub-Saharan African KS patients and 22 KSHV-infected asymptomatic individuals (ASY), and ultimately to support an application …

The Impact Of Iron Supplementation On The Preterm Neonatal Gut Microbiome: A Pilot Study, Matthew Van Ormer, Maranda Thompson, Melissa K. Thoene, Jean-Jack Riethoven, Sathish Kumar Natarajan, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Objective The gastrointestinal microbiome in preterm infants exhibits significant influence on optimal outcomes–with dysbiosis shown to substantially increase the risk of the life-threatening necrotizing enterocolitis. Iron is a vital nutrient especially during the perinatal window of rapid hemoglobin production, tissue growth, and foundational neurodevelopment. However, excess colonic iron exhibits potent oxidation capacity and alters the gut microbiome–potentially facilitating the proliferation of pathological bacterial strains. Breastfed preterm infants routinely receive iron supplementation starting 14 days after delivery and are highly vulnerable to morbidities associated with gastrointestinal dysbiosis. Therefore, we set out to determine if routine iron supplementation alters the preterm gut …

Hybridization And Gene Expression: Beyond Differentially Expressed Genes, Anna Runemark, Emily C. Moore, Erica L. Larson

School of Biological Sciences: Faculty Publications

Gene expression has a key role in reproductive isolation, and studies of hybrid gene expression have identified mechanisms causing hybrid sterility. Here, we review the evidence for altered gene expression following hybridization and outline the mechanisms shown to contribute to altered gene expression in hybrids. Transgressive gene expression, transcending that of both parental species, is pervasive in early generation sterile hybrids, but also frequently observed in viable, fertile hybrids. We highlight studies showing that hybridization can result in transgressive gene expression, also in established hybrid lineages or species. Such extreme patterns of gene expression in stabilized hybrid taxa suggest that …

Taxonomic Notes On The Cladodinae, Lampyrinae, And Lampyrinae/Lampyridae Incertae Sedis (Coleoptera: Lampyridae), Oliver Keller, Gavin J. Martin

Insecta Mundi

We propose several nomenclatural changes for taxa in the subfamily Cladodinae and the lampyrid tribes Cratomorphini, Lamprocerini, Lampyrini, Photinini, and Pleotomini in the subfamily Lampyrinae (Coleoptera: Lampyridae). We present evidence for the correct year of description of Calotrachelum flavolineatum Pic, 1941, Diaphanes lateapicalis breveapicalis Pic, 1954, Photinus conradti Pic, 1940, and all species described by Charles Émile Blanchard. Spellings are corrected for Diaphanes lateapicalis breveapicalis Pic, 1954, Diaphanes latipennis dartevillei Pic, 1952, Diaphanes longecarinatus Pic, 1955, Diaphanes moultoni latemarginatus Pic, 1938, Lampyris olivieriana von Heyden, 1890, and Photinus reductemarginalis Pic, 1941. We propose Lucidota puertoestrellaensis Keller and Martin nomen novum …