Life Sciences Commons^™

5-Year Strategic Direction, 2024–2028: Institute Of Agriculture And Natural Resources, University Of Nebraska–Lincoln, Institute Of Agriculture And Natural Resources University Of Nebraska-Lincoln

Institute of Agriculture and Natural Resources: Institute Publications

IANR: Integrating Learning, Discovery, and Engagement for an Even Better Nebraska

Includes an introduction, description of the components of the Institute of Agriculture and Natural Resources at the University of Nebraska–Lincoln, the IANR pillars, stratgeic aims for 2024–2028, and a section on implementation.

Public Horticulture: Process And Design Of The Lincoln Botanical Garden, Brad Kindler

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Beginning in 2012, the local non-profit, The Lincoln Botanical Garden, began organizing the conceptual design and creation of a glass conservatory within a botanical garden in Lincoln, Nebraska. These proposed urban enhancements seek to fill a gap in garden amenities in the city by providing year-round access to public horticulture programming and education.

This project, Public Horticulture: Process and Design of the Lincoln Botanical Garden, documents these efforts and makes design recommendations for a themed Glacial Erratic Garden that could be constructed within the botanical garden.

Advisors: Ellen Paparozzi and Sam Wortman

Stories Of Motivation And Values Of Small-Scale Farmers In Jamaica And The United States, Raquel Taylor

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Small-scale farmers add to global and local food systems through various means such as connecting communities to local food and support economies. Small-scale farms can address issues related to food and nutritional security. However, farming can be a challenging career to engage in, especially for those who are small-scale farmers. Matters related to succession, higher paying off-farm employment, policies, and more can make it challenging to engage in farming. Leaving the question of what would motivate someone to engage in small-scale farming. Motivation can be viewed through multiple lenses; this study chose to use self-determination theory to understand the motivations …

Impact Of Plant Defenses And Aphid-Associated Microbes In Modulating Sorghum–Sugarcane Aphid Interactions, Edith Ikuze

Department of Entomology: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor) is a vital cereal crop globally, facing challenges from invasive pests such as the sugarcane aphid (SCA; Melanaphis sacchari). To elucidate mechanisms of sorghum resistance to SCA, we examined the role of plant defenses and the influence of aphid-associated microbes in modulating sorghum defenses. Lignin is a complex heterogenous polymer present in nearly all plant cell walls that plays a critical role in protecting plants from various stresses. In this study, we investigated the role of the caffeoyl coenzyme-A O-methyltransferase (CCoAOMT), one of the core enzymes in monolignol biosynthesis pathway that leads to lignin …

Vibratory Noise Impacts The Sensory Ecology Of A Funnel-Weaving Spider, Brandi June Pessman

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Animals experience their world through diverse sensory systems, leveraging information from prey, predators, and potential mates to inform behavioral decisions. Environmental noise disrupts information via spectral or amplitude masking, degradation, or distortion, potentially leading to costly errors in responses. Critically, urbanization has accelerated the proliferation of novel noise sources, triggering significant shifts in communication, foraging, and predator-prey dynamics among urban wildlife. While vertebrate studies have illuminated urban animals’ flexibility to anthropogenic acoustic noise, a comprehensive understanding requires broader investigations across diverse animal taxa and sensory modalities. To address these gaps, my dissertation explored the impacts of anthropogenic substrate-borne (vibratory) noise …

Genome-Scale Metabolic Modeling Of Chitin-Degrading Microbial Systems, Aimee Kristin Kessell

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

As a major component of fungal cell walls and exoskeletons of invertebrates, chitin is widespread in soils, constituting the second most abundant biopolymer in nature. Composed of N-acetyl-D-glucosamine chains, it serves as a vital source of nutrients, including both carbon and nitrogen, for the growth of microorganisms. A solid understanding of the microbial degradation of chitin is critical for predicting their impacts on biogeochemical cycling in soil ecosystems. Organisms that degrade biopolymers (degraders) produce energetically expensive extracellular enzymes to break down complex organic carbons into simpler labile forms that are sharable with other species, including those that do not contribute …

Analyzing Phototaxis And Related Visual Behaviors Among Diverse Species Of Drosophila, Madeline M. Hill

Honors Program: Senior Projects (Public)

Phototaxis and related visual behaviors can vary between species, and thus members of the genus Drosophila make an excellent study system to examine the evolution of vision. While some existing research points to these phototactic behaviors arising due to mating requirements or due to their species-specific ecology or environmental factors, there exists a lack of understanding as to why striking behavioral differences can exist between closely related species, or between members belonging to the same genus. The present research seeks to uncover the specifics regarding these discrepancies in visual evolution and aims to provide a foundation of knowledge about visual …

Sarcopenia And The Importance Of Resistance Training And Protein-Rich Diets For Prevention Of Muscle Loss In Older Adults: A Literature Review And Informational Pamphlet, Madison Steele

Honors Program: Senior Projects (Public)

The growing prevalence of sarcopenia among older adults as well as youths has prompted further research aimed at developing effective preventative measures and treatment plans. Sarcopenia is often thought to occur solely due to aging, but several other factors contribute to progressive muscle loss. Previous research studies have found an effect of physical activity and dietary protein levels on the risk of developing sarcopenia as well as its onset age. This study aims to review existing knowledge in the field to compile an extensive list of sarcopenia causes and methods of prevention. The main causes identified in this review are …

Proposing Urban Agroforestry Designs For Lincoln, Nebraska: A Model From Berlin, Germany, Noah Johnson

Honors Program: Senior Projects (Public)

Given the threat of a worsening climate crisis, there is a strong need for community and ecosystem resilience. Diverse urban agroforestry systems have the potential to accomplish both and meet many of the objectives outlined in the city of Lincoln, Nebraska’s climate action plan. Additionally, Berlin, Germany could provide an effective model for Lincoln in this regard given the city’s extensive history of established urban agroforestry systems. The objective of this study then is to develop a design for an urban agroforestry site modeled on Berlin’s allotment gardens and tailored to Lincoln’s needs. The methods for creating this design included …

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby

Honors Program: Senior Projects (Public)

With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H₂ that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …

Ecological Dynamics Of Cenozoic North American Mammals, Alexandria Brandis Shupinski

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding how community structure is affected by various ecological, climatic, and environmental changes is a long-standing goal in ecology. However, disentangling human impacts from these other variables is difficult when using short modern timescales and anthropogenically altered communities. The fossil record provides a means to investigate community structure dynamics across critical intervals, excluding humans as a variable. Here, in three studies, I assess changes in North American mammal paleocommunity structure across the last 66 million years (Ma) at multiple temporal and spatial scales. In Chapter 1, I identify changes in mammal functional diversity (FD) locally and continentally, using three independent …

Investigating Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome Using Mitotalens, Megan Gusso

Honors Program: Senior Projects (Public)

This lab project explores the complexity of the mitochondrial genome by investigating large genomic repeats and their effect on flowering plants’ reproduction and viability. Mitochondria are the site of cellular respiration to create energy and contain their own DNA. This is counterintuitive because the byproducts of cellular respiration impose a threat of mutation for the stored DNA. The mitochondrial genome differs in size between animals and plants. Plant mitochondrial DNA is larger due to the presence of large, noncoding repeated sequences, and a large amount of DNA of unknown function. These sequences undergo high levels of recombination and rearrangement. The …

Irt-Based Irrigation Scheduling And Dry Bean Crop Water Productivity Under Different Irrigation Scenarios In The Nebraska High Plains, Angie Gradiz

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Irrigation plays a critical role for crop production in western Nebraska of the United States, where crop evapotranspiration often exceeds in-season precipitation. This study investigated the impacts of different irrigation treatments on yield and key physiological parameters of dry edible beans grown in semi-arid western Nebraska during the 2022-2023 growing seasons. Seven irrigation levels (0%, 25%, 50%, 75%, 100%, 125%, 150%) of the fully irrigated treatment (FIT), were applied using a Variable Rate Irrigation (VRI) Linear-Move Sprinkler System. Additionally, an eighth treatment was scheduled based on measurement from infrared radiometry thermometers (IRT) to evaluate the performance of the two-source energy …

Toxicological Effects And Treatment Of Accidental Ingestion Of Simparica Trio® In Felis Catus: An Investigative Review, Aussia M. Stander

Honors Program: Senior Projects (Public)

Simparica TRIO^®, a canine endectocide manufactured by Zoetis, contains sarolaner, moxidectin, and pyrantel pamoate salt in an orally administered chewable tablet. These compounds are all toxic to both cats and dogs in sufficient amounts. As Simparica TRIO^® rises in popularity among pet owners, Felis catus (felines, cats) in multi-pet households (households with both cats and dogs) are at increased risk of overdosage and toxicosis from accidental ingestion of Simparica TRIO^® due to their smaller weight.

Simparica TRIO^® is not for feline use and little literature exists on the symptoms of and treatment for accidental ingestion …

Consequences Of The Megafauna Extinction: Changes In Food Web Networks On The Edwards Plateau Across The Pleistocene–Holocene Transition, Quentin A. Smith Jr.

School of Biological Sciences: Dissertations, Theses, and Student Research

We are experiencing biodiversity loss due to climate change and human impacts, which is not only harmful to the environment but can also alter the composition of communities and interactions among species. The late Pleistocene experienced a loss of large-bodied mammals which resulted in significant changes in community structure due to changes in body size, diet, and species associations. The impact of these changes on species interactions and community structure across the Pleistocene-Holocene transition remains poorly understood. Using a robust data set of species composition, stable isotopes, body size, and climate variables, we constructed and compared ecological networks of mammal …

The Impact Of A Pgym Variant On Myophosphorylase Deficiency In Red Angus Composite Cattle And Changes In The Skeletal Muscle Transcriptome Due To The Intramuscular Administration Of Lidocaine In Wether Lambs, Mackenzie Christin Batt

School of Biological Sciences: Dissertations, Theses, and Student Research

Project 1 focused on eight calves in a Nebraska herd (composite Simmental, Red Angus, Gelbvieh) that displayed exercise intolerance during forced activity. Available sire pedigrees contained a paternal ancestor within 2-4 generations in all affected calves. Pedigrees of the calves’ dams were unavailable, however, the cows were ranch-raised and retained from prior breeding seasons, where bulls used for breeding occasionally had a common ancestor. Therefore, it was hypothesized that a de novo autosomal recessive variant was causative of exercise intolerance in these calves. A genome-wide association analysis followed by whole-genome sequencing led to the identification of a variant in the …

Impact Of Strategic Postweaning Supplementation On March- And May-Born Yearling Range Heifer And Calf Growth And Performance In The Nebraska Sandhills, Josie Nicole Crouch

Department of Animal Science: Dissertations, Theses, and Student Research

Three experiments were conducted at the Gudmundsen Sandhills laboratory near Whitman, Nebraska. Experiment 1, a retrospective regression analysis, evaluated the impact of heifer percentage of mature body weight (BW) at breeding on heifer performance, subsequent pregnancy rates, and calf production. Overall pregnancy rates were greater (P < 0.01) for heifers at 60 to 70% of mature BW than 50 to 55%, with no differences in pregnancy rates (P ≥ 0.15) after 2-years of age. Heifers at 50 to 60% of mature BW had a greater (P = 0.05) proportion give birth in the first 21-d. A higher percentage of mature BW at breeding resulted in greater (P < 0.01) BW at birth and weaning for offspring.

Experiments 2 and 3 were 3-year studies that evaluated the impact …

Artificial Insemination And Fetal Programming In Beef Production Systems, Dempster M. Christenson

Department of Animal Science: Dissertations, Theses, and Student Research

Pregnancy to artificial insemination and late gestation fetal programming of the next calf crop are central to genetic development, reproductive longevity, calf crop productivity, and efficiency of cow/calf ranching. The purposes of this research are to find methods to improve pregnancy rate to artificial insemination, the growth and reproductive health of pregnant heifers, and the productivity of their offspring. In the first of four studies we found that extending the period of progesterone administration in an estrus synchronization protocol did not significantly hasten estrus response or increase pregnancy rate to artificial insemination, but timing of estrus within the melengestrol acetate …

Adding Parasite Resistance To A Hair Sheep Breeding Objective, Robert M. Forbes

Department of Animal Science: Dissertations, Theses, and Student Research

The United States Hair Sheep Index was designed to increase total weight of lamb weaned per ewe lambing (TW) using estimated breeding values (EBV) for direct (DWWT) and maternal (MWWT) weaning weight, and number of lambs born (NLB) and weaned (NLW), as selection criteria. Producers are interested in adding post-weaning fecal egg count EBV (PFEC), an indicator of parasite resistance, to the index to achieve genetic change in both TW and PFEC.

First, the effects of adding PFEC alongside TW to the breeding objective of indexes that used either phenotypes or EBV as selection criteria were studied. Since MWWT …

Feeding Dried Distillers' Grains With Solubles To Lactating Dairy Cattle: Whole Animal Energy Utilization And Manure Biogas Production, Grant Michael Fincham

Department of Animal Science: Dissertations, Theses, and Student Research

When DDGS are fed to lactating dairy cattle, the observed response in energy supply, milk production, and methane production varies among studies. One potential reason for these observed discrepancies is the nature of the diet formulation itself. Furthermore, little research has been conducted to examine how dietary changes can affect dairy cattle manure composition and methane production in an anaerobic manure digester. In order to gain a better understanding of these topics, we conducted two experiments. The first experiment was conducted to test how the manipulation of lactating dairy cattle diets affects energy utilization, milk production, methane production, and manure …

The Future Of The Organization Of The Petroleum Exporting Countries, Mikayla Gross

Honors Program: Senior Projects (Public)

The Organization of the Petroleum Exporting Countries (OPEC) is a multinational and intergovernmental organization that dictates the petroleum exportation policies of 13 countries: Algeria, Angola, Equatorial Guinea, Gabon, Iran, Iraq, Kuwait, Libya, Nigeria, the Republic of the Congo, Saudi Arabia, the United Arab Emirates and Venezuela (About Us, 2023). The mission of OPEC is to ensure a stable supply of petroleum through its shared policies for its Member Countries and consumers (Our Mission, 2023). Iran, Iraq, Kuwait, Saudi Arabia, and Venezuela founded OPEC in 1960 under the foundations and principles that dictated the international world order. Those foundations and principles …

Utilization Of The NuTrack System To Objectively Evaluate Changes In Active And Passive Behaviors Of Group-Housed Nursery Pigs Exposed To An Endotoxin Challenge, Aaron Holliday

Department of Animal Science: Dissertations, Theses, and Student Research

This study aimed to evaluate the changes in activity of group-housed, newly weaned pigs challenged with lipopolysaccharide (LPS). At weaning, pigs (n = 192, 5.73 ± 1.8 kg) were stratified by sex, litter, and body weight (BW) and randomly assigned to one of three treatments (16 pigs/pen, 4 pens/treatment): 1) Saline-injected (SAL), 2) 50% challenged [50%-LPS) – only half of the pigs in a pen (8 pigs) were challenged with LPS and 3) 100% challenged (100%-LPS) – all pigs in a pen were challenged with LPS. Pigs in the SAL and 32 in the 50%-LPS treatment received a 3.0 …

Using Annual Forages To Replace Declining Cool-Season Grass Pasture Availability, Jonathon Matthew Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As land area in grain crop production increases in the US Midwest, perennial pasture availability decreases but the demand increases. Matching the seasonal growth patterns of C₃ and C₄ plant species to grow forages that are complementary allows producers to fill production gaps in otherwise monoculture systems and possibly increase overall productivity. One way to fill the forage production gap of C₃ grass dominated pastures is by producing a warm-season, annual forage in a separate field. However, with continual reductions in land availability, this may not be an option. Using two studies, our objectives were to improve …

Evaluation Of Essential Oils And Their Major Components For Disease Resistance, Serkan Tokgöz

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Essential oils (EOs) are natural hydrophobic compounds, which are highly complex, typically comprising 20 to 60 constituents, with two or three main components making up 20% to 95% of their composition, alongside other minor constituents. Research has firmly established the potent antibacterial and antifungal properties of EOs and their primary components. To assess their efficacy, one EO, nutmeg oil, and seven main components, including safrole, toscanol, safraleine, cinnamaldehyde, carvacrol, carvone, and thymol, were tested against four economically significant fungal pathogens (Alternaria solani, Fusarium graminearum, Rhizoctonia solani, and Sclerotiorum sclerotiorum), one oomycete pathogen (Phytium ultimum …

Next-Generation Crop Monitoring Technologies: Case Studies About Edge Image Processing For Crop Monitoring And Soil Water Property Modeling Via Above-Ground Sensors, Nipuna Chamara

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Artificial Intelligence (AI) has advanced rapidly in the past two decades. Internet of Things (IoT) technology has advanced rapidly during the last decade. Merging these two technologies has immense potential in several industries, including agriculture.

We have identified several research gaps in utilizing IoT technology in agriculture. One problem was the digital divide between rural, unconnected, or limited connected areas and urban areas for utilizing images for decision-making, which has advanced with the growth of AI. Another area for improvement was the farmers' demotivation to use in-situ soil moisture sensors for irrigation decision-making due to inherited installation difficulties. As Nebraska …

An Investigation Of Information Structures In Dna, Joel Mohrmann

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

The information-containing nature of the DNA molecule has been long known and observed. One technique for quantifying the relationships existing within the information contained in DNA sequences is an entity from information theory known as the average mutual information (AMI) profile. This investigation sought to use principally the AMI profile along with a few other metrics to explore the structure of the information contained in DNA sequences.

Treating DNA sequences as an information source, several computational methods were employed to model their information structure. Maximum likelihood and maximum a posteriori estimators were used to predict missing bases in DNA sequences. …

Concepts In Animal Parasitology: Master Bibliography, Sue Ann Gardner

Concepts in Animal Parasitology

Master bibliography for the open educational resource/open access textbook Concepts in Animal Parasitology, Scott L. Gardner and Sue Ann Gardner, editors, published by Zea Books, Lincoln, Nebraska, United States, 2024. This includes the references from literature cited and suggested supplemental reading.

Notes On North American Butterflies, 1, Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

New natural history elements and distribution records of several North American butterflies are reported. Taxonomy is often driven by geographical analyses. Cartography, coupled with internet-based imagery, opens up a promising wealth of information, allowing avocational researchers a tool by which inconvenient visits to institutional collections are no longer necessary for resolving some basic questions. While diversity and distribution of butterflies in North America are commonly believed to be fully known, the findings presented here show that much is yet to be learned of our butterfly fauna.

Ectoparasites Of The Critically Endangered Green Sawfish Pristis Zijsron And Sympatric Elasmobranchs In Western Australia, Jack Ingelbrecht, Karissa O. Lear, Storm B. Martin, Alan J. Lymbery, Bradley M. Norman, Geoffrey A. Boxshall, David L. Morgan

Harold W. Manter Laboratory of Parasitology: Library Materials

This study reports the metazoan ectoparasite fauna of juvenile Critically Endangered green sawfish, Pristis zijsron, and sympatric elasmobranchs in Western Australia. Five parasite taxa were found on 76 screened P. zijsron: Caligus furcisetifer (Copepoda: Caligidae), Dermopristis pterophila (Monogenea: Microbothriidae), Branchellion plicobranchus and Stibarobdella macrothela (Hirudinea: Piscicolidae), and praniza larvae of an unidentified gnathiid isopod. Only C. furcisetifer and D. pterophila were common, exhibiting discrepant site-specificity, with C. furcisetifer occurring mostly on the head and rostrum, and D. pterophila around the pectoral and pelvic fins. Intensity of infection for C. furcisetifer and D. pterophila increased with host total length and …

An Examination Of The Eastern Nebraska And Western Iowa Flash Flood Event Of 6-7 August 1999, Catherine M. Zapotocny

National Oceanographic and Atmospheric Administration: Technical Reports and Related Materials

Flash flooding occurs each summer in eastern Nebraska and western Iowa. While 10-inch rains are extraordinary, it is not uncommon to have at least one excessive rain event during the annual convective season. A significant flash flood occurred in east central Nebraska and western Iowa the night of August 6, 1999 into the morning of August 7, 1999. Antecedent hydrologic conditions limited the loss of life, however considerable property damage resulted as the heavy rain fell over the urbanized areas in eastern Nebraska and western Iowa.

Several flash flood ingredients came together over the area. These meteorological factors are typical …