Life Sciences Commons^™

Annual Forage Insurance Program Performance, Jay Parsons

Cornhusker Economics

In 2014, the Annual Forage Insurance Program (AFIP) was offered for the first time by the USDA – Risk Management Agency (RMA) as a pilot program for the states of Nebraska, North Dakota, South Dakota, Kansas, Oklahoma, and Texas. It was expanded to include Colorado in 2016 and New Mexico in 2017. This year marks the 10-year anniversary of the program, and the goal of this article is to provide a summary of where the program is and how it is being used. We will focus specific attention on Nebraska but also provide an overview of the national picture.

The …

Records Of The Red-Bellied Snake (Storeria Occipitomaculata) In Central And Northeastern Nebraska, Taylor Lowe, Sarah M. Bailey, Dan D. Fogell, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Red-bellied Snakes (Storeria occipitomaculata) are a distinctive, small species that live in moist woodlands throughout much of eastern North America. In the Great Plains, isolated populations exist in South Dakota, Wyoming, and Nebraska. In Nebraska, the only known extant population occurs in wooded riparian habitats along the Big Bend Reach of the Platte River in southcentral parts of the state. Herein, we report on new county records of Red-bellied Snakes in Nebraska, with two observations from Merrick County along the Platte River adjacent to known populations and two observations of a new population in the extreme northeastern part …

Greater Aperture Counteracts Effects Of Reduced Stomatal Density On Water Use Efficiency: A Case Study On Sugarcane And Meta-Analysis, Daniel Lunn, Baskaran Kannan, Amandine Germon, Alistair Leverett, Thomas E. Clemente, Fredy Altpeter, Andrew D.B. Leakey

Department of Agronomy and Horticulture: Faculty Publications

Stomata regulate CO₂ and water vapor exchange between leaves and the atmosphere. Stomata are a target for engineering to improve crop intrinsic water use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) and reduce stomatal conductance (g_sw). However, the quantitative relationship between reduced SD, g_sw, and the mechanisms underlying it is poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as a case study alongside a meta-analysis of data from 10 species. Transgenic expression of EPIDERMAL PATTERNING FACTOR 2 from Sorghum bicolor (SbEPF2) in sugarcane …

Assessment Of Genetic Diversity And Population Structure Of U.S. Polypay Sheep From Breed Origins To Future Genomic Selection, Carrie S. Wilson, Jessica Lynn Petersen, Luiz F. Brito, Brad A. Freking, Sara M. Nilson, J. Bret Taylor, Thomas W. Murphy, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Knowledge of past and present genetic diversity within a breed is critical for the design and optimization of breeding programs as well as the development of strategies for the conservation of genetic resources. The Polypay sheep breed was developed at the U.S. Sheep Experiment Station (USSES) in 1968 with the goal of improving productivity in Western U.S. range flocks. It has since flourished in the more intensively managed production systems throughout the U.S. The genetic diversity of the breed has yet to be documented. Therefore, the primary objective of this study was to perform a comprehensive evaluation of the genetic …

Lymphoid Tissues Contribute To Plasma Viral Clonotypes Early After Antiretroviral Therapy Interruption In Siv-Infected Rhesus Macaques, Antonio Solis-Leal, Nongthombam Boby, Suvadip Mallick, Yilun Cheng, Fei Wu, Grey De La Torre, Jason Dufour, Xavier Alvarez, Vinay Shivanna, Yaozhong Liu, Christine M. Fennessey, Jeffrey D. Lifson, Qingsheng Li, Brandon F. Keele, Binhua Ling

School of Biological Sciences: Faculty Publications

The rebound-competent viral reservoir, composed of a virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation of systemic viral replication and rebound viremia after ART interruption (ATI), remains the biggest obstacle to treating HIV infection. A better understanding of the cellular and tissue origins and the dynamics of viral populations that initiate rebound upon ATI could help develop therapeutic strategies for reducing the rebound-competent viral reservoir. In this study, barcoded simian immunodeficiency virus (SIV), SIVmac239M, was used to infect rhesus macaques to enable monitoring of viral barcode clonotypes contributing to virus detectable in plasma after ATI. …

Revisiting Fr13a For Submergence Tolerance: Beyond The Sub1a Gene, Waseem Hussain, Mahender Anumalla, Abdelbagi M. Ismail, Harkamal Walia, Vistas Kumar Singh, Ajay Kohli, Sankalp Bhosale, Hans Bhardwaj

Department of Agronomy and Horticulture: Faculty Publications

Rice landrace FR13A is the original donor of the SUB1A gene. FR13A harbors hidden genetic variation that surpasses the submergence tolerance level of SUB1A. Here, we provide an overview of the unique features of FR13A associated with exceptional recovery ability that have been overlooked since the significant discovery of the SUB1 locus. We present a detailed overview of how to underpin the new genes associated with the recovery ability seen in FR13A. Finally, we propose a unique approach to develop rice cultivars that surpass submergence tolerance beyond the SUB1 locus from FR13A.

Evaluation Of The Capacity And Effective Angle Of Thalamic Damage For 2 Commercially Available Captive Bolt Tool Types On Cadaver Heads From Sows >200 Kg Body Weight, Karly N. Anderson, Ruth Woiwode, Ashlynn A. Kirk, Jennifer Berger, Arquimides A. Reyes, Perle E. Zhitnitskiy, Kurt D. Vogel

Department of Animal Science: Faculty Publications

This study evaluated the ability of 2 penetrating captive bolt (PCB) types (PISTOL, INLINE) to reach and disrupt the thalamus when applied in 2 placements (FRONTAL, BEHIND EAR) to chilled cadaver heads (N = 60) from sows >200 kg. Heads were randomly distributed across 6 treatments (n = 10): FRONTAL-INLINE, FRONTAL-PISTOL, FRONTAL-NO SHOT, BEHIND EAR-INLINE, BEHIND EAR-PISTOL, and BEHIND EAR-NO SHOT. The FRONTAL shot was placed 3.5 cm superior to the optic orbits at the midline; the BEHIND EAR shot was placed directly caudal to the pinna of the ear on the same plane as the eyes and targeting the …

The Growth Of Ethanol In Brazil: Sugarcane, Corn, And A Few Other Things, Fabio Mattos

Cornhusker Economics

Although we can expect more ethanol produced from corn in the coming years, it is unclear whether it will keep expanding at the same pace as in recent years or how much it will contribute to total ethanol production.

One point to keep an eye on is the typical dynamics of markets. If supply and demand conditions and price movements make corn-ethanol more profitable, ethanol plants in Brazil will keep increasing their production. If it makes more economic sense to produce corn-ethanol (as it happened recently), they will use more corn to produce ethanol.

Supply and demand conditions for ethanol, …

Using Machine Learning To Determine A Functional Classifier Of Reward Responsiveness And Its Association With Adolescent Psychiatric Symptomatology, Robert James Richard Blair, Johannah Bashford-Largo, Ahria Dominguez, Matthew Dobbertin, Karina S. Blair, Sahil Bajaj

Center for Brain, Biology, and Behavior: Faculty Publications

Background. Machine learning (ML) has developed classifiers differentiating patient groups despite concerns regarding diagnostic reliability. An alternative strategy, used here, is to develop a functional classifier (hyperplane) (e.g. distinguishing the neural responses to received reward v. received punishment in typically developing (TD) adolescents) and then determine the functional integrity of the response (reward response distance from the hyperplane) in adolescents with externalizing and internalizing conditions and its associations with symptom clusters.

Methods. Two hundred and ninety nine adolescents (mean age = 15.07 ± 2.30 years, 117 females) were divided into three groups: a training sample of TD adolescents …

Second Language Learning In Older Adults Modulates Stroop Task Performance And Brain Activation, Douglas H. Schultz, Alison Gansemer, Kiley Allgood, Mariah Gentz, Lauren Secilmis, Zoha Deldar, Cary R. Savage, Ladan Ghazi Saidi

Center for Brain, Biology, and Behavior: Faculty Publications

Introduction: Numerous studies have highlighted cognitive benefits in lifelong bilinguals during aging, manifesting as superior performance on cognitive tasks compared to monolingual counterparts. Yet, the cognitive impacts of acquiring a new language in older adulthood remain unexplored. In this study, we assessed both behavioral and fMRI responses during a Stroop task in older adults, pre- and post language-learning intervention.

Methods: A group of 41 participants (age: 60–80) from a predominantly monolingual environment underwent a four-month online language course, selecting a new language of their preference. This intervention mandated engagement for 90 minutes a day, five days a week. …

A Two-Part Project, Phil Warburton

Department of Entomology: Distance Master of Science Projects

1) an analysis of the constraints to the use of entomological observations collected by citizen scientists for research

The online database, iNaturalist, is transforming observational entomology with a massive and valuable database of photographed insect sightings in their environment. Increasingly, iNaturalist is becoming a rich source of scientific data for research purposes. However, iNaturalist faces a hidden but potentially serious crisis-in-the making. The phenomenal growth threatens to outstrip the capacity of expert insect identifiers.

As the database grows rapidly, the rate of identification of insect observations to the “Research Grade” standard is in decline. Furthermore, the number of expert identifiers …

Archaeoentomology: Arthropods And Humans, Current Works In Archaeoentomology, Paz Vigil

Department of Entomology: Distance Master of Science Projects

This paper presents a brief literature review, exploring human and insect interaction, and how such research aids the ongoing construction of the archaeological and entomological record. This field, a combined subset of both entomology and archaeology, is both complex and rich as well as young and still growing. This literature review is not meant to be an exhaustive or comprehensive exploration of this subject, but it will serve as part of a foundation for those interested in the topic or wanting to conduct further exploratory study in this field. The intent is to highlight the practical applications of Archaeoentomology, using …

Evaluating A Precision Sprayer For Detecting Weeds And Spraying Herbicides In Real Time (Spot Spray) For Weed Management In Corn And Soybean, Adam Eric Leise

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide sprayers are becoming larger and more efficient in applications in crop fields. A precise and efficient herbicide application is advantageous in effective weed control. New precision sprayers were introduced in hopes of reducing the amount of herbicide applied. Greeneye Technology and John Deere have developed precision sprayers for the detection of weeds in crop fields, which come equipped with a dual tank system that allows for two different herbicides to be separated from tank to tip. The objectives of this study were to evaluate the broadleaf and grass weed control efficacy of spot spray (SS) technology when applied in …

Biostratigraphic Revision Of Esthonyx (Tillidontia, Mammalia) In The Contect Of Climate Change In The Lower Eocene Of The Bighorn Basin, Wyoming, John Colter Johnson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Esthonyx is an early Eocene tillodont found in North America, Europe, and India. The genus was named by Edward Drinker (E. D.) Cope in 1874 based on specimens from the San Jose Formation in New Mexico. Since then, several species of Esthonyx have been described from North America alone. The best record of Esthonyx comes from the central Bighorn Basin (BHB) in Wyoming, where it appears in 220 localities in a 640 m-thick stratigraphic succession. Since the last summary of Esthonyx in the BHB, several hundred new specimens have been collected and curated at the Denver Museum of Nature and …

Examining Dietary Disparities And Associated Factors Among Young Children Across Diverse Racial, Ethnic And Immigrant Households In Nebraska, Naveta Bhatti

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Early childhood is a crucial stage for developing eating behaviors which in turn plays a major role in determining health outcomes. Assessing children’s diet plays a crucial role in tackling the obesity epidemic. One of the ways to assess children’s dietary intake is by evaluating their dietary quality. Dietary quality refers to the overall healthfulness and nutritional value of an individual's diet, considering the combination of foods consumed over time. Research has consistently demonstrated that a high-quality diet is associated with a reduced risk of several health issues, such as all-cause mortality, cardiovascular disease incidence or mortality, cancer incidence or …

Unmanned Aerial Vehicle (Uav)-Based High-Throughput Phenotyping For Maize Improvement, Eric T. Rodene

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Modern breeding programs rely heavily on efficiently screening large numbers of genotypes for agronomic traits, such as disease resistance, drought tolerance, and yield. Identifying the genetic loci or genes associated with these traits using GWAS or functional analyses will benefit future plant breeding efforts seeking to incorporate these traits into new crop varieties, whether through conventional breeding or gene editing techniques. Unmanned aerial vehicle (UAV)-based image data has been increasingly used for this task, as it allows entire test plots to be quickly and cost-effectively phenotyped. In my research, I have developed methods to improve the accuracy of machine learning …

Performance Fatigability And Neuromuscular Responses During And After One Set Of Forearm Flexion Muscle Actions To Failure At 30% Versus 70% One-Repetition Maximum In Men And Women, Tyler Joseph Neltner

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Previous studies have examined sex differences in performance fatigability during isometric, isokinetic and controlled dynamic constant external resistance (DCER) fatiguing tasks (FT), however, whether those sex differences remain during maximal intended velocity (MIV) DCER muscle actions remains unclear. The purpose of this study was to examine the sex-related differences in the time course of fatigue, neuromuscular responses, and coactivation from the ipsilateral and contralateral limbs during MIV DCER muscle actions to failure at low versus high relative loads, as well as performance fatigability from pre- to post-testing. Twenty-seven college-aged men (n = 14) and women (n = 13) …

Identifying The Relative Abundance Of Cell-Cell Signaling Peptides During Mammalian Hibernation And Anesthetic Administration By Label-Free Peptidomics Analysis, Somayeh Mousavi

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cell-cell signaling molecules known as neuropeptide and peptide hormones, distributed in both the central nervous system (CNS) and the peripheral nervous system (PNS), regulate a variety of physiological processes including body temperature, feeding, circadian rhythm, reproduction, energy homeostasis, and more. While a number of active neuropeptides structures with physiological functions have been discovered, advances in peptidomic mass spectrometry are enabling researchers to uncover novel, previously unknown, active neuropeptide structures with potential for bioactivities. Using liquid chromatography coupled with tandem mass spectrometry (MS/MS), it is possible to identify a wide range of neuropeptides in single experiments without preselecting specific peptides. Mass …

Exploring Producers' Willingness To Pay For Ecosystem Services: Three Essays On Soil Health, Location Preferences, And Cover Crop Adoption, Kaouter Essakkat

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation comprises three essays: one assessing Midwest producers' willingness-to-pay (WTP) for soil health-enhancing ecosystem services (ES), another examining the impact of policy implementation locations on WTP, and a third identifying factors influencing producers' adoption of cover crops (CC).

The first essay pioneers the understanding of agricultural producers’ valuation of soil health-associated ES amid growing concerns of soil degradation due to agricultural intensification. Using a discrete choice experiment, this study assesses producers’ WTP for a policy that incentivizes ES provision, specifically improved water quality, carbon sequestration, and enhanced crop yield. Surveying producers across Iowa, Kansas, and Nebraska, our results show …

Assessment Of Foam Decontamination In Tomato Washing Process, Basim M. Alohali

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The work of the dissertation investigated the efficacy of foam-based decontamination treatments to enhance microbial safety in Roma tomatoes, specifically targeting natural microflora and Salmonella contamination. Growing concerns over foodborne illnesses linked to fresh tomatoes and the limitations of conventional decontamination techniques, such as organic matter built-up and limited microbial reduction, are addressed. The primary objectives were to characterize the physiochemical properties of foams formed using various concentrations of Tween 20 (T20) and sodium dodecyl sulfate (SDS) with and without the addition of sodium hypochlorite (NaOCl) and peracetic acid (PAA), compare the efficacy of foam and traditional dump tank (DT) …

Manipulations Of The Caenorhabditis Elegans Microbiome Reveal Influences Of Detrimental Members On Host Life History Traits, Ashley Elizabeth Foltz

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Microbiomes are a fundamental part of eukaryotic organisms and play a role in both host health and fitness. Although microbiomes are often associated with health and positive effects on the host, certain microbiome compositions are also associated with disease. We used Caenorhabditis elegans and its experimental microbiome, CeMbio to explore the interactions that individual microbiome members have with other microbes, with the host, and how the entire microbiome community interacts with the host. We found that individual CeMbio members have varying effects on host health and fitness compared to the whole community. This suggested that there is synergy occurring in …

Development Of A Durable And Low-Dose Universal Vaccine Against Swine Influenza A Virus, Erika Petro-Turnquist

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Swine Influenza A Virus (IAV-S) imposes a significant burden on the pork industry and poses a great threat to public health due to its zoonotic potential. Susceptibility to human, avian, and swine influenza make pigs the ultimate mixing vessels for reassorted influenza A virus strains. Current vaccination strategies induce short-lived and strain-specific responses against IAV-S. Further, the substantial genetic diversity of IAV-S necessitates consistent revaccination and updating of commercial vaccines. Computational platforms have recently emerged as a promising strategy to develop new-age vaccines against infectious diseases in livestock animals. Here, we describe a computationally derived set of vaccine immunogens, called …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle Duncan Dougherty

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Genetically Modified Bovine Milk-Derived Small Extracellular Vesicles In Drug Delivery, Alice Kah Hui Ngu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Small extracellular vesicles (SEVs, “exosomes”) are natural nanoparticles that can be endogenously synthesized and also obtained from dietary sources, such as bovine milk SEVs (mEVs). mEVs have been extensively studied and discovered with contributive features that potentially play a role in drug delivery and other therapeutic purposes, such as diagnostic tools. Current studies have shown that mEVs have been used preclinically to carry in targeting drugs, nucleotides, biomolecules, and labels to target metabolic diseases and cancers. Extensive studies have focused on further modifying SEVs to enhance their versatility in pharmaceutical use. In this dissertation, we exhibited one of the ways …

Population Demography, Occupancy, And Connectivity Of Invasive Bigheaded Carp In The Platte River, Nebraska And Its Tributaries, Blake W. Logan

School of Natural Resources: Dissertations, Theses, and Student Research

Silver Carp Hypophthalmichthys molitrix and Bighead Carp H. nobilis, collectively referred to as bigheaded carp, are invasive species in the United States of America that have expanded their range throughout the Missouri River basin in the last three decades. It may be desirable to develop management plans directed at the monitoring, control, or removal of bigheaded carp within the Missouri River basin. However, a knowledge gap exists regarding bigheaded carp ecology in Missouri River tributaries, which may be important for the development of management plans. As such, this study characterized bigheaded carp population demographics, occupancy, and connectivity in …

Virtual Fencing In Livestock Management: Effects On Beef Cattle Stress And Enhancing Wildlife Conservation, Kaitlyn E. Dozler

School of Natural Resources: Dissertations, Theses, and Student Research

I examined the effects of virtual fencing on beef cattle stress and how implementing the technology can aid wildlife habitat conservation at the University of Nebraska Gudmundsen Sandhills laboratory (GSL) near Whitman, NE. First, I examined how virtual fencing influences acute heart rate changes in beef cattle fitted with the virtual fencing collars. No significant difference in the average heart rate of a cow (P > 0.05) was observed between the 30 min control and virtual fenced periods for both trial dates. Second, I examined the influence of topography on wildlife species richness and presence at GSL using wildlife camera …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle D. Dougherty

School of Natural Resources: Dissertations, Theses, and Student Research

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Hybridization Between The Rare Gray-Headed Chickadee And The Abundant Boreal Chickadee In The Midst Of Shifting Climate, Matthew R. Armstrong

School of Natural Resources: Dissertations, Theses, and Student Research

As species respond to changing climate, distributions and abundances may shift and alter species interactions. Hybridization, a relatively widespread phenomenon becoming more common with climate change, can have beneficial and detrimental effects on population growth rates and genetic integrity. Beneficial effects due to the introduction of advantageous alleles and increased genetic diversity may result from hybridization. Species may also accrue fitness costs associated with changing climates if mismatches occur between environmental variables and phenotypes. The gray-headed chickadee, Poecile cinctus lathami, is an extremely rare songbird that has experienced marked declines in recent decades within its restricted distribution in Alaska …

Phylogeny And Disparity Of Ammonoid Family Acanthoceratidae Over Ocean Anoxic Event 2, Lindsey Howard

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

The widespread use of genera as proxies for species in paleobiological studies might affect the results of these studies. Although most attention has been given to taxonomic diversity studies, this could also be true of disparity and phylogenetic studies. In particular, the assumption that particular character states truly diagnose all members of a genus might distort results. This study examines the disparity of Acanthoceratid ammonoids at both the generic and species level. 149 species from 42 genera were examined with 52 characters measured. Following the measurements, an inverse modeling simulation was run 100 times to generate a simulated phylogeny with …

Integrating Remote Sensing And Machine Learning To Determine Past, Current And Future Crop Water Use From The Nubian Sandstone Aquifer System, Moaz Ishag

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The agriculture sector is a significant consumer of water, and sustainable water use begins with monitoring irrigated land. Delineating irrigated land supports decision-makers and promotes the sustainable use of this crucial resource. This study focuses on the Nubian Sandstone Aquifer System (NSAS), the largest aquifers in the world, which spans Egypt, Sudan, Libya, and Chad. The study aims to: 1) quantify the increase in irrigated hectares (both pivot and non-pivot) from 2000-2001 to 2023-2024; 2) identify major irrigated crop types and their water requirements; and 3) quantify groundwater crop water use from the NSAS using remote sensing via the Google …