Life Sciences Commons^™

A Course Correction? The Correlation Between Gfsi-Recognized Certifications And Salmonella In Raw Chicken Products, Kar Ho Lim, Lijiao Hu, Yuqing Zheng, Michael Ollinger

Agricultural Economics Faculty Publications

The Global Food Safety Initiative (GFSI) is a widely recognized entity. Many third-party certifications, such as Safe Quality Food (SQF), Brand Reputation through Compliance Global Standard (BRCGS), and Food Safety System Certification 22000 (FSSC), benchmarked against GFSI standards. This study compares the Salmonella detection rates in raw chicken products from SQF, BRCGS, FSSC-certified establishments to those from uncertified ones. We performed a probit regression and calculated average marginal effects using testing data from the Food Safety Inspection Service. Overall, products from certified establishments are associated with 3.2 % lower positive rate of Salmonella detection. Certification is associated with a greater …

Anuran Occupancy Of Created Wetlands Within Reforested Legacy Surface Mines In Kentucky And West Virginia, Jillian C. Newman, Steve Price, Jacquelyn C. Guzy, Joshua Castle, John J. Cox, Jeffery L. Larkin, Christopher D. Barton

Forestry and Natural Resources Faculty Publications

Restoration of habitat is an important component of wildlife management. Surface coal mining has altered forest cover in the Central Appalachians, and most reclamation activities on previously mined lands result in non-native grasslands or shrub lands. The Forestry Reclamation Approach (FRA), which emphasizes non-native vegetation removal, soil decompaction, planting of native trees and shrubs, and, in some cases, wetland creation represents an alternative to grassland-based reclamation that may promote forest-associated wildlife on legacy surface mines. We used automated recording units to evaluate the response of anurans to created wetlands and the FRA in Kentucky and West Virginia, USA. We used …

Microglial Apoe Across Alzheimer’S Disease-Relevant Pathologies, Georgia Nolt

Theses and Dissertations--Physiology

APOE exists as three common alleles (APOE2, APOE3, APOE4) that encode their corresponding protein isoforms (apoE2, apoE3, apoE4). Despite differing by only one or two single-amino acid substitutions, each APOE allele confers a distinct AD risk profile with APOE4 increasing risk by 28% and APOE2 decreasing risk by 14% compared to the most commonly expressed APOE3 allele. While traditionally considered “neutral,” APOE3 still accounts for ~36% of AD cases while APOE4 contributes ~57%, indicating that the majority of AD cases would not occur in the absence of the APOE3 and APOE4 alleles. Microglia, the resident phagocytic immune cells …

Field-Scale Evaluation Of Satellite- Derived Vegetation Indices And Image Timing For In-Season Nitrogen Management In Corn, Ana Morales-Ona, Katsutoshi Mizuta, James J. Camberato, Robert Nielsen, Yuxin Miao, Davide Cammarano, German Mandrini, Daniel Quinn

Plant and Soil Sciences Faculty Publications

Introduction: Traditional methods to determine the agronomic optimum nitrogen rate (AONR) for corn (Zea mays L.) rely on grain yield data, limiting in-season decision-making for nitrogen (N) management. Vegetation indices (VIs) derived from satellite imagery can serve as proxies for grain yield and help estimate in-season AONR, enabling timely sidedress applications. This study aimed to (1) quantify how VI–yield relationships vary during the vegetative period across fields with different tillage systems and crop residue; (2) determine whether VI–N response curves can be used to estimate AONR (AONRvi); and (3) assess the accuracy of AONRvi relative to yield-based AONR (AONRy). …

Exploring The Relationships Between Social Influence, Vaping Behaviors, And Susceptibility Across Grade Levels In Kentucky, Raphael Adesiyan, Odunayo O. Omotilewa, Saber Feizy, Melinda J. Ickes

Kinesiology and Health Promotion Faculty Publications

Purpose: This study explored the relationships between social influence, vaping behavior, and susceptibility across different grade levels.   Design: The study design was cross-sectional. Data were collected from middle and high school students who voluntarily participated between September 2022 and April 2023.   Sample: A convenience sample of students in 6^th to 12^th grades in Kentucky (N = 4677).   Measures: Demographic information, including age, grade level, school location, and race/ethnicity, was collected. The outcome variables included social influence, susceptibility, and vaping behavior.   Analysis: Logistic regression analysis was used to explore the relationships between social influence, vaping behavior, and susceptibility.   Results: …

Chinese Athletes’ And Parents’ Motivations For U.S. Collegiate Sports Participation, Wenting Sun, Russell D. Ravert, Simran Kaur Sethi

Kinesiology and Health Promotion Faculty Publications

To gain an understanding of international student recruitment and retention in collegiate athletics, this study examines the motivations and decision-making processes of Chinese high school athletes and their parents in pursuing U.S. collegiate sport opportunities. Using the push–pull framework and an ecological perspective, it explores how individual aspirations, family priorities, institutional resources, and national policies shape transnational mobility. Semistructured focus group discussions with 10 athletes and eight parents revealed six key motivational themes and highlighted the influence of recent collegiate sport-related policy changes in China. The findings suggest that while both groups value U.S. sport pathways, athletes and parents may …

Climate Change Effects On Biomass And Greenhouse Gas Emissions Are Ameliorated By Nontoxic Endophytes In Southeastern Usa Transition Zone Tall Fescue Pastures, Rebecca Kay Mcgrail, Jonathan D. Moore, A. Elizabeth Carlisle, Jim A. Nelson, Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Background: The plant–microbe interaction between tall fescue and its endophyte Epichloë coenophiala can affect ecosystem responses to changing rainfall patterns and increasing temperatures. Endophyte-infected (E+) stands often exhibit improved stress tolerance and differing soil greenhouse gas (GHG) emissions compared to endophyte-free (E−) stands. However, it is unknown if mammalian nontoxic endophyte (NTE) strains introduced into some tall fescue cultivars confer differing stress tolerances or reduce soil GHG emissions.

Methods: We quantified the impact of fescue-NTE symbioses in Jesup and Texoma on biomass and GHG emissions in response to increased temperature (+3°C) and altered precipitation (50% lengthened interval between events) using …

A Global Synthesis Of Fragipan Silicon Concentrations And Relationships With Soil Physicochemical Properties As A Function Of Soil Parent Materials, Christopher Shepard

Plant and Soil Sciences Faculty Publications

Fragipans are dense, brittle subsurface horizons that restrict root growth and limit water-holding capacity. A common factor in these soils is a binding agent, potentially either phyllosilicate clay bridging, amorphous aluminosilicate/silica, or silica sorbed to Fe oxides. Others have suggested that no binding agent is present within these soil horizons and that the arrangement of individual particles is solely responsible for these horizons. In this article, a secondary data synthesis was utilized to demonstrate that silicon (Si) accumulation is a key pedogenic process in loess-derived fragipan horizons, but not in all fragipans. The average dithionite-extractable Si (Si_d) content …

Disrupted Circadian Rhythms Affect Hallmark Pathologies In Alzheimer’S Disease-Related Mouse Models, Valeria Buzinova

Theses and Dissertations--Molecular and Cellular Biochemistry

Alzheimer’s Disease (AD) is a complex neurodegenerative disease with two hallmark pathologies: extracellular amyloid-b (Ab) and intracellular neurofibrillary tangles (NFTs). Ab is proteolytically processed from amyloid precursor protein (APP) by b-secretase and g-secretase as a monomeric peptide prone to aggregation. Eventually, the aggregate-prone monomers will form dense plaques that are difficult to break down and remove. These plaques begin to deposit into the cortex decades prior to the formation of NFTs and the onset of cognitive decline. NFTs are comprised of hyper-phosphorylated tau. Tau is a protein that serves to promote and stabilize the formation of microtubules. The formation of …

Characterizing The Human Metapneumovirus Matrix Protein And Its Role In Infection, Chase J. Heim

Theses and Dissertations--Molecular and Cellular Biochemistry

Human metapneumovirus (HMPV) causes severe respiratory tract infections in all cohorts, but especially in vulnerable groups such as children, older adults, and the immunocompromised. The matrix (M) protein of HMPV, like the M proteins of other members of the Mononegavirales order, is involved in virus assembly and budding. However, other functions of the HMPV M protein have yet to be elucidated. To investigate these various functions of the M protein, we used a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) antisense agent designed to block translation of the M gene to reduce M expression during infection. Treatment with varying concentrations of an …

The Role Of Small Extracellular Vesicles In Modulating Radiation Resistance In H3k27m-Pediatric Diffuse Midline Glioma, Viral Oza

Theses and Dissertations--Molecular and Cellular Biochemistry

Pediatric diffuse midline gliomas with H3K27M alteration (H3K27M-pDMG) are the leading cause of pediatric brain tumor-associated deaths. All H3K27M-pDMG are initially treated with fractionated radiotherapy, the standard of care, and most children succumb to their disease within two years of diagnosis. There are no universally effective chemotherapies and full resection is impossible due to the diffuse nature of the tumor and sensitive location. There has not been a significant clinical advancement in more than 40 years.

Tumors become completely resistant to radiation within the first six months of treatment. The mechanism of radiation resistance is unknown but is thought to …

A Major Update And Improved Validation Functionality In The Mwtab Python Library And The Metabolomics Workbench File Status Website, P. Travis Thompson, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

Background: The Metabolomics Workbench (MW) is a public scientific data repository consisting of experimental data and metadata from metabolomics studies collected with mass spectroscopy (MS) and nuclear magnetic resonance (NMR) analyses. Although not as rapidly as in the past, MW has steadily evolved, updating its mwTab and JSON deposition text file formats and its web-based infrastructure. However, the growth of MW has been exponential since its inception in 2013 and continues to be exponential, with the number of datasets hosted on the repository increasing by 50% since April 2024. As part of regular maintenance to keep up with changes to …

The Effect Of Cover Crop Management Strategies On Nutrient Availability In No-Till Corn, Emily C. Marsh

Theses and Dissertations--Plant and Soil Sciences

Cover crops are reported to have long-term soil health improvements, one of which is reducing erosion. However, popular cereal cover crops, such as rye (Secale cereale L.), have the potential to cause a yield penalty in the following corn (Zea mays L.) crop due to a reduction in available nitrogen. Legumes, such as Austrian winter pea (Pisum sativum L. var. arvense), are thought to reduce this yield penalty due to their capacity to fix nitrogen. Sulfur is another nutrient of interest, especially as rates of atmospheric deposition of sulfur have declined, leading to more deficiencies being …

Soil Solarization As A Sustainable Approach To Manage Weeds And Arthropod Pests, And Its Effects On Soil Nitrate And Lettuce (Lactuca Sativa L.) Yield In Kentucky And Tennessee High Tunnels, Paula Luize Lessmann

Theses and Dissertations--Plant and Soil Sciences

The use of high tunnels (HTs) has increased over the past 15 years, with more than 1,700 and 700 structures currently in Kentucky and Tennessee, respectively. High tunnels are widely used to extend the growing season and protect specialty crops from adverse weather conditions. However, continuous production and limited crop rotation within HTs can lead to the accumulation of soilborne pathogens, plant-parasitic nematodes, arthropod pests, and weeds, ultimately reducing crop yield and quality. Management of these biotic issues is particularly challenging in HTs due to limited pesticide options compared to open field production. Soil solarization, a non-chemical method that uses …

Modeling Inherited Retinal Disease In Zebrafish, Meet Patel

Theses and Dissertations--Biology

Inherited retinal diseases (IRDs) affect millions of people worldwide. Majority of IRDs are caused by degeneration of rod and cone photoreceptor cells (PRCs) due to gene mutations. The overarching goal of my dissertation is to model and evaluate the molecular role of various gene candidates involved in IRDs such as cone rod dystrophy (CRD) and retinitis pigmentosa (RP).

Mutations in CDHR1, a photoreceptor specific cadherin have been associated with CRD and recapitulated in mouse CDHR1 knockouts. However, the molecular function of CDHR1 remains unknown. CDHR1 has been shown to localize at the leading edge of murine rod nascent outer segment …

Kentucky Bluegrass (Poa Pratensis L.) As A Perennial Groundcover: Establishment And Weed Management In Corn, Kiera S. Searcy

Theses and Dissertations--Plant and Soil Sciences

Perennial groundcover (PGC) systems offer a cost-effective alternative to annual cover crops, by providing continuous soil cover, reducing soil erosion, and contributing to integrated weed management (IWM) in corn (Zea mays L.) production systems. Kentucky bluegrass (Poa pratensis L.) has been proposed as a perennial groundcover for corn due to its low growth habit and seasonal dormancy; however, successful adoption is limited by challenges associated with establishment-phase weed competition and compatibility with weed management programs during corn production. The objectives of this research were to (1) evaluate weed management strategies that support Kentucky bluegrass establishment and (2) assess …

Integrated Crop Management For Hemp (Cannabis Sativa L.) Grain Production In Kentucky, Maria M. Ricciardi

Theses and Dissertations--Plant and Soil Sciences

Cannabis sativa L. is legally defined (U.S.) as hemp if the total tetrahydrocannabinol (THC) concentration remains less than 0.3% on a dry weight basis. Hemp can be produced for cannabinoid extraction, fiber, or grain. Hempseed meal and hempseed oil were approved as feed ingredients for chickens and performance horses in Kentucky. To leverage this opportunity, production requires improvement through an integrated crop management approach. This dissertation (1) reports the main arthropod and disease pests impacting hemp grain; (2) identifies grain yield components and their relationship with corn earworm, Helicoverpa zea (Boddie) (CEW); (3) evaluates Fusarium head blight (FHB) development across …

Breeding For Fhb Resistance And Post-Harvest Quality In Soft Red Winter Wheat, Margaret E. Gillum

Theses and Dissertations--Plant and Soil Sciences

Soft red winter wheat (Triticum aestivum L.) is both an economically and nutritionally important crop in the global food supply. Despite its global importance, wheat is continually threatened by fungal diseases. Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating fungal disease affecting wheat worldwide, resulting in low yields, reduced test weight and mycotoxin production. The mycotoxin deoxynivalenol (DON) is toxic to humans, results in feed rejection in livestock, and is heavily monitored in the food supply. Because of wheat’s importance in the food system, post-harvest quality (milling, baking, and flavor) has been a growing area of …

Evaluating Spatial Variability Of Corn Yield Response To Phosphorus Fertilizer For Improved Precision Phosphorus Management, Jennifer Fridgen

Theses and Dissertations--Plant and Soil Sciences

Soil scientists did not develop soil test-based fertilizer recommendations to support precision nutrient management. Knowledge of crop responses to applied essential nutrients, such as phosphorus (P), and the processes governing these responses at high spatial resolution are necessary to improve precision agronomic recommendations. This dissertation investigates the limitations of current soil sampling methodologies and recommendation frameworks for crop response to fertilizer, with particular emphasis on P fertility in Kentucky (KY) corn production. The first study examined the relationship between the resolution of systematic grid sampling and the spatial structure of soil test variables (Mehlich-3 P (M3P), Mehlich-3 potassium (M3K), and …

Effect Of Substrate Dispersion Method And Particle Size On In Vitro Digestibility, Gas Production Kinetics And Composition, And Fermentation Characteristics In Different Feed Types, Luiz C. O. Sousa, Ellem M. A. Matos, Ronald J. Trotta, Edenio Detmann, David L. Harmon

Animal and Food Sciences Faculty Publications

Our objective was to evaluate the influence of substrate dispersion method and particle size on in vitro digestibility, gas production kinetics and composition, and fermentation characteristics of different feed types. Alfalfa hay, tall fescue hay, ground corn, soybean meal, and a total mixed ration (TMR) were used as substrates. Treatments were designed according to a 2 × 2 × 5 factorial arrangement: two substrate dispersion methods (loose substrate and in filter bags), two particle sizes (1 mm and 2 mm), and five feeds. Filter bags decreased (P ≤ 0.001) digestibility and gas production compared with loose samples. Filter bags decreased …

Menstrual Cycle Phase Effects On Alcohol-Impaired Driving Performance And Risk Awareness, Annie K. Griffith

Theses and Dissertations--Psychology

Growing evidence indicates that risky behavior among women is elevated during the late follicular phase of the menstrual cycle, likely due to increased estradiol levels. Furthermore, alcohol-induced impairment of women’s inhibitory control is significantly greater during the late versus early follicular phase. Consequently, cycle phase may influence outcomes of poor inhibitory control while intoxicated, such as alcohol-impaired driving. Alcohol-impaired driving risk manifests in two ways: risk-taking while driving, and risk awareness regarding the decision to drive. This dissertation tested the hypothesis that alcohol-induced increases in driver risk-taking and decreases in risk awareness would be exacerbated during the late versus early …

Daily Rhythms In Psychosocial Stress, Sleep, And Mood: A Multimethod Approach To Transdiagnostic Psychopathology Risk And Intervention, Gabriel Gilmore

Theses and Dissertations--Psychology

Rhythms are central to nearly every core physiological and psychological process and have profound influences on overall health and well-being, particularly in the context of mood disorders. This dissertation emphasizes a rhythm-centered framework for understanding transdiagnostic psychopathology, proposing that the temporal organization of psychosocial stress, sleep, and mood provides crucial information for the development and treatment of mood disorder symptomology. Current literature relies primarily on mean symptom levels, overlooking day-to-day patterns in psychosocial experiences. To address this key methodological gap, three studies are examined. Study 1 develops and validates the Psychosocial Stress Regularity Measure (PSRM), a brief single-item measure of …

Comparing Strength Testing Measures And The Association Of Strength And Body Composition In Young Children, Abigayle R. Spong

Theses and Dissertations--Kinesiology and Health Promotion

Muscle strength is an important component of physical fitness; however, measuring it with isokinetic dynamometry (IKD) is expensive and requires dedicated space. Handheld dynamometry (HHD) provides a practical alternative for field and clinical settings. Thus, the purpose of this study was to compare peak knee flexion and extension torque measured by IKD and HHD in 37 children (ages 5-11 years) and examine relationships with body composition. Participants completed a RAMPing protocol at 90^o knee flexion consisting of two warm-up contractions at 50% and 75% effort followed by three maximal voluntary isometric contractions. Body composition was assessed via DXA scans. …

Biomechanical Effects Of Exercise-Based Interventions In Chronic Nonspecific Low Back Pain: A Systematic Review And Meta-Analysis, Lara De Vecchi Machado

Theses and Dissertations--Kinesiology and Health Promotion

Chronic nonspecific low back pain (CNLBP) is the leading cause of years lived with disability worldwide and represents a complex, multifactorial, and heterogeneous condition. It is characterized by substantial interindividual variability in muscle activation patterns, lumbo-pelvic coordination, and movement strategies. Although exercise is widely recommended as a first-line intervention and its clinical benefits are well established, the biomechanical mechanisms underlying functional improvement remain fragmented in the literature and are rarely synthesized quantitatively.

This dissertation conducted a systematic review with meta-analysis to investigate the biomechanical effects of exercise-based interventions in individuals with CNLBP. A total of 39 studies were included and …

Coexistence Between Ci And Feminization Inducing Symbionts: A Theoretical Perspective, Dylan Thorp

University of Kentucky Master's Theses

Endosymbiotic bacteria that manipulate the reproduction of their host are abundant in arthropods. Theoretical efforts to model these bacteria have seldom included symbionts that feminize their hosts, and those that do have assumed an environmental sex determination system. Here, I expand upon other theoretical works to model the dynamics of a host population with an XX/X0 sex determination system that contains separate CI and feminization inducing symbionts. I found that coexistence cannot occur without double infection, consistent with previous models. However, I also found that without double infection, the presence of the feminizing symbiont can facilitate the spread of the …

17Β Estradiol Protection Against Medium-Change Neurotoxicity In Organotypic Hippocampal Slice Cultures, Autumn B. Stage

University of Kentucky Master's Theses

Medium change neurotoxicity can disrupt glutamate homeostasis and promote excitotoxic neuronal injury through excessive NMDA receptor activation. 17β estradiol (E2) has demonstrated neuroprotective effects in models of neuronal injury and may regulate glutamatergic signaling through metabotropic glutamate receptors (mGluR2/3). This study investigated whether E2 protects against medium change associated neurotoxicity in organotypic hippocampal slice cultures whether responses differed by sex or hippocampal region. Hippocampal slices from postnatal day 8 male and female Sprague Dawley rats were maintained in culture and exposed to 1, 10, or 100 nM E2 for 24 hours following a five day recovery period. Propidium iodide (PI) …

Introducing Neuro Social Work: A Brief Reflection On Neuroscience And The Profession, Priscila Llamosa

Social Work Faculty Publications

Neuroscience offers language and evidence that can strengthen interdisciplinary communication, support advocacy in biomedically dominated settings, and validate relational practices long recognized in social work, including the impacts of early adversity, attachment, and healing through connection. At the same time, an epistemological tension underlies the profession’s unease with neuroscience when brain-based narratives are used to individualize structural harm or justify surveillance of marginalized families and communities. This teaching note introduces Neuro Social Work as a justice-oriented specialization that integrates contemporary neuroscience with social work’s person-in-environment commitments, relational practice traditions, and anti-oppressive ethics.The author argues that the key question is no …

Genetic Engineering For Pastures, W J. Peacock

IGC Proceedings (1977-2023)

The first genetically engineered food products from horticultural production systems are now entering the mnrke< and field trials of genetically engineered crop species are well advanced. We cnn expect that a range of crop cultivars, in which gene

Grassland For Sustainable Ecosystems, Malcolm Hadley

IGC Proceedings (1977-2023)

Grasslnnds present many advantages for examining issues related to sustainability. Grasslands include planted pastures as well as a variety of natural rangelands in situations ranging from semi-arid to subhumid. Diversity is also reflected in the spectrum of grassland users and uses, for example, nomadic pastoralism, extensive ranches, intensive pastures, wildlife tourism, game hunting. And in terms of sustainability of resource management, grasslands encompass a wide range of contrasting situations and conflicts in resource use. Heavy grazing pressure associated with increasing human populations in certain tropical savannas, the implications of rural depopulation to traditional pasture management in alpine regions, and the …

Grasslands For Our World, F Riveros

IGC Proceedings (1977-2023)

The present-day role of grasslands and fodder is reviewed with respect agriculture, animal production and environmental concerns. The world's grazing lands are a finite resource and, overall, arc being badly managed; in most developing countries action is required now, before it is too late. They will continue to be a major source of animal feed for the foreseeable future; encroachment from cropping will continue locally but, for much of what is now natural pasture, exploitation by the grazing animal .will remain the major means of obtaining an economic return; the task of grassland professionals is to assist the managers of …