Life Sciences Commons^™

Matrix Geochemistry And Phytophthora Occurrence On Reforested Mine Lands In Appalachia, Kathryn M. Ward

University of Kentucky Master's Theses

At the Bent Mountain surface mine, Pike County, Kentucky, a study has been ongoing since 2005 to assess the influence of various types of loose-graded mine spoils on water quality and forest establishment. Six research plots consist of two replicates of brown weathered sandstone, gray unweathered sandstone, and mixed brown sandstone, gray sandstone, and shale that were emplaced according to Forestry Reclamation Approach criteria. A series of analyses was initiated in 2007 to examine influence of spoil matrix composition on sulfate and carbonate geochemistry of infiltrated waters, as well as to investigate the occurrence of Phytophthora, a group of …

Influence Of Various Plant Populations On Weed Removal Timing In Glyphosate-Resistant Soybean, Jason Sarver

University of Kentucky Master's Theses

Reduced plant population in glyphosate-resistant soybean [Glycine max (L) Merr.] may influence the critical time of herbicide application. Field studies were conducted in 2007 and 2008 at two locations in Kentucky to determine the effect of four weed control programs on soybean seed yield, seed quality, crop canopy, and weed pressure when planted at three densities - 185,000, 309,000, and 432,000 plants ha^-1. Plots were treated with glyphosate at either 3 weeks after planting (WAP), 5 WAP, 7 WAP, 3 & 7 WAP, representing common weed control protocols within the state. No differences in seed yield were …

The Importance Of Nutrition Label Usage In The Context Of Obesity: A Cross-Country Study Of The Usa And Turkey, Emine Bayar

University of Kentucky Master's Theses

Obesity, the second leading cause of preventable death in the U.S., and related health problems increase people’s concerns about healthy food consumption. The increased prevalence of obesity is a major concern of societies both in developed and developing countries. Nutrition label usage has been increasing due to the link between diet and health. This study intends to provide a framework for describing profiles of consumers who are more likely to use nutrition labels in USA and Turkey, a developing country with increasing obesity rates in recent years. Empirical results present similarities and differences between consumers’ attributes for food label usage …

Effects Of Augmented Real-Time Auditory Feedback On Top-Level Precision Shooting Performance, Stacy Marie Underwood

University of Kentucky Master's Theses

This study examined the effects of training with real-time auditory feedback in precision shooting. Top-level shooters (N=9) were randomly assigned to the feedback or nonfeedback group. Each group performed a pre-test, a 4-week training intervention and a post-test. The feedback group was provided with augmented real-time auditory feedback based on postural and rifle barrel stability during training sessions. Increases in performance were measured through changes in postural stability, rifle barrel stability, shot outcome and shot group diameter. Real-time auditory feedback did not increase postural or rifle barrel stability in the feedback group. No meaningful differences were found related to shot …

Hip Muscle Strength And Pelvic Obliquity In Collegiate Females During Walking And Stair Descent Tasks, Kelly Rodriguez

University of Kentucky Master's Theses

The goals of the pelvis include maintaining the center of mass of the body, assisting in foot clearance and absorb forces from the lower extremities using muscles and ligaments to stabilize the joint. A better understanding of the influence of muscle strength on controlling pelvic obliquity in a healthy population will help in understanding low back pain and overuse lower extremity injuries. Thirteen females (22 ±2 yrs) participated in isokinetic strength testing of the hip abductors, adductors, internal rotators and external rotators on a Biodex dynamometer. The subjects also underwent gait analysis during self selected pace walking and stair descent. …

Furthering Pharmacological And Physiological Assessment Of The Glutamatergic Receptors At The Drosophila Neuromuscular Junction, Junyoung Lee

University of Kentucky Master's Theses

Drosophila larval neuromuscular junctions (NMJs) serve as a model for synaptic physiology. The molecular sequence of the postsynaptic glutamate receptors has been described; however, the pharmacological profile has not been fully elucidated. Despite the postsynaptic molecular sequence used to classify the receptors as a kainate subtype, they do not respond pharmacologically as such. Kainate does not depolarize the muscle, but dampens evoked EPSP amplitudes. Quantal responses show a decreased amplitude and area under the voltage curve indicative of reduced postsynaptic receptor sensitivity to glutamate transmission. ATPA, a kainate receptor agonist, did not mimic kainate’s action. The metabotropic glutamate receptor agonist …

Florida Panther And Black Bear: A Road And Urban Avoidance/Utilization Analysis And Impacts Of Land Use And Climate Change On Large Carnivore Habitat In Florida, Andrew James Whittle

University of Kentucky Master's Theses

Florida is expanding its urban borders into areas of the native habitat. Increased expansion is predicted through the next several decades. Several sections of the state are home to large carnivores, such as Florida panther and black bear, which are important to ecosystem function. Expansion of roads and urban centers will greatly reduce the quality and quantity of carnivore habitat. In this study, I used Euclidean distance analyses and very high frequency (VHF) telemetry points to produce distance categories in which carnivores either have a negative/neutral/positive association with roads and urban centers. The seven black bear populations followed four different …

The Impact Of Perceived Barriers To Export: An Analysis Of Kentucky Agricultural And Food Processing Firms, Kelly A. Davidson

University of Kentucky Master's Theses

As intra-industry trade increases in U.S. agricultural and food processing industries, the historical agricultural trade surplus is tightening. In efforts to maintain the trade surplus a focus has shifted towards the promotion of agricultural and processed food exports among small and medium sized firms. This study intends to identify and evaluate the potential for exports among small to medium sized agricultural and food processing firms in Kentucky through a collection of survey data. The objectives of this thesis are to identify the state’s product marketing opportunities and product specifications for international exports while identifying transaction requirements for potential exports. An …

School Lunch Programs And The American Diet: Exploring A Contested Food Terrain, Rebecca Som Castellano

University of Kentucky Master's Theses

This study examines the social actors and issues involved in constructing and contesting the National School Lunch Program (NSLP), in order to identify whose interests are involved in shaping an institution which transmits dietary habits and food knowledge to the nation’s children through the mid day meal.

For the historical analysis, I collected data from historical accounts of the NSLP, congressional hearings, laws, and newspaper articles. For the contemporary analysis, I interviewed 15 actors representing organizations key to federal NLSP policy making. To frame my analysis, I utilize a model of power, based on the work of Arts and Van …

An Interaction Between Risk Perceptipton And Trust In Response To Food Safety Events Across Products And Regions, And Their Implicaitons For Agribusiness Firms, Jonathan D. Shepherd

University of Kentucky Master's Theses

Food safety events receive substantial media coverage and can create devastating economics losses for agribusiness firms. It is unclear what factors influence consumers’ purchasing decisions before or after a food safety event occurs. The objectives of this study is to identify these factors that influence purchasing decisions, determine how consumers respond to hypothetical food safety events, and compare these findings across different products and geographical regions. The data for this research was obtained from two surveys. One survey concerned fresh produce while the second focused on meat products. The SPARTA model, based on the Theory of Planned Behavior, is used …

Influences Of Supplementing A Mannan Oligosaccharide Containing Product To Pig Diets On Sow And Weanling Pig Performance, I-Fen Hung

University of Kentucky Master's Theses

The objective of these studies was to evaluate the effect of supplying mannan oligosaccharides (MOS) to pig diets on both weanling pig and sow performances.

Nursery pigs challenged with LPS to stimulate an immune challenge had increased body temperature and respiratory rate as well as elevated serum cortisol and TNF-α concentration, MOS-supplemented pigs had a lower rectal temperature and respiratory rate which implied that MOS improves some aspect of the immune function of piglets.

MOS supplementation in sow diets during late gestation and lactation had no effect on litter size, but the piglets from MOS-fed sows were heavier at birth …

Evaluation Of Dietary Alterations That Have Potential To Affect Feed Intake And Feed Preference In Swine, James Seth Monegue

University of Kentucky Master's Theses

Feed intake is a key factor affecting pig performance; thus, the objective of these studies was to assess a variety of factors that could potentially affect intake in pigs in different production stages. Studies were conducted to determine the effects of flavor and diet complexity, Appetein™ (an alternative protein source), and graded levels of salt on swine feed intake and feed preference. Two newly developed flavors were used in nursery pig diets. The use of the two flavors did not increase feed intake (P > 0.05). Nursery pigs actually showed a preference for the control diet. Complex diet formulation does increase …

Down-Regulation Of Ppargamma1 Suppresses Cell Growth And Induces Apoptosis In Mcf-7 Breast Cancer Cells, Yekaterina Y. Zaytseva, Xin Wang, R. Chase Southard, Natalie K. Wallis, Michael W. Kilgore

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Peroxisome proliferator-activated receptor gamma (PPARgamma) is a member of the nuclear hormone receptor superfamily and is highly expressed in many human tumors including breast cancer. PPARgamma has been identified as a potential target for breast cancer therapy based on the fact that its activation by synthetic ligands affects the differentiation, proliferation, and apoptosis of cancer cells. However, the controversial nature of current studies and disappointing results from clinical trials raise questions about the contribution of PPARgamma signaling in breast cancer development in the absence of stimulation by exogenous ligands. Recent reports from both in vitro and in vivo studies …

Forage News [2008-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Improved Grass Varieties Benefit Farmers
• Alfalfa and the Environment
• Grass May Help Balance Hot Dairy Diets
• Ethanol Maker Files for Bankruptcy Relief
• Pricing Hay: Consider Plant Nutrient Value
• Performance and Physiology of Steers Grazing Toxic Tall Fescue as Influenced by Feeding Soybean Hulls and Steroidal Implants
• Upcoming Events

Borderline Personality Disorder: An Overview Of History, Diagnosis And Treatment In Adolescents, Linah Al-Alem, Hatim A. Omar

Pharmacology and Nutritional Sciences Faculty Publications

Borderline personality disorder (BPD) is a cluster B personality disorder. It is characterized by erratic behaviors, emotional instability and one of its hallmarks is self injurious behavior, which starts in adolescence. Patients with BPD are difficult to treat, most have a history of child sexual abuse, about a quarter present with sexual abuse from a caretaker. Although personality disorders are diagnosed only in adults, BPD manifests itself in adolescence in the form of uncontrollable anger, self mutilations, dissociation and other such behaviors. Hence, there is a growing number of scientists discussing the possibility of diagnosing BPD in adolescents. Here, we …

Pgrmc1: A New Biomarker For The Estrogen Receptor In Breast Cancer, Rolf J. Craven

Pharmacology and Nutritional Sciences Faculty Publications

Estrogen receptor (ER) status is a critical biomarker in breast cancer, in large part because the ER is the target of tamoxifen and similar drugs. In the previous issue of Breast Cancer Research, Neubauer and colleagues used a proteomic approach to identify proteins that are differentially regulated by ER in breast tumors. The authors showed that ER-negative tumors have elevated levels of PGRMC1 (progesterone receptor membrane component-1), a hormone receptor component and binding partner for P450 proteins. In contrast, PGRMC1 was phosphorylated in ER-positive tumors. The staining patterns of ER and PGRMC1 were mutually exclusive in breast tumor sections, and …

Forage News [2008-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Awards
• Clayton Geralds Wins KFGC Forage Spokesman Contest
• 29th Alfalfa Conference Program Set
• Forages at KCA
• 9th Grazing Conference Proceedings
• Decision Aid Added to Website
• Enough Hay…
• Animal Grazing Habits
• Time to Focus On Switchgrass Economics
• Limiting Access Time to Hay Can Stretch the Roll
• Horse Gastric Ulcer Syndrome Can Be Controlled With Diet
• Upcoming Events

Technical Variables In High-Throughput Mirna Expression Profiling: Much Work Remains To Be Done, Peter T. Nelson, Wang-Xia Wang, Bernard R. Wilfred, Guiliang Tang

Pathology and Laboratory Medicine Faculty Publications

MicroRNA (miRNA) gene expression profiling has provided important insights into plant and animal biology. However, there has not been ample published work about pitfalls associated with technical parameters in miRNA gene expression profiling. One source of pertinent information about technical variables in gene expression profiling is the separate and more well-established literature regarding mRNA expression profiling. However, many aspects of miRNA biochemistry are unique. For example, the cellular processing and compartmentation of miRNAs, the differential stability of specific miRNAs, and aspects of global miRNA expression regulation require specific consideration. Additional possible sources of systematic bias in miRNA expression studies include …

Forage Spokesman Information, Clayton Geralds, Brady Jarvis

Kentucky Grazing Conference

No abstract provided.

Supplementing Cattle On Pasture: When, What And How Much?, Roy Burris

Kentucky Grazing Conference

The biggest advantage of maintaining cattle in the southeast is our ability to grow forages. It is generally an economic advantage to make maximum use of our forage and then supplement only when needed to meet our production goals. Judicious use of extra nutrition to grazing cattle can allow us to meet an objective which was best stated by Hoveland in 1986:

“Our objective is to maintain pasture at the highest quality that the environment will allow and efficiently convert as much of the pasture as possible into saleable animal product.”

Fencing And Watering Systems: Simpler Is Better, Ralph Quillin

Kentucky Grazing Conference

Sustainability and Grazing Management have taken on a new importance in the Americana farm community. The key components of the sustainable approach, profitability, agronomic, social and ecological responsibility and quality of life are becoming obtainable goals for this and future farming generations.

Practical Grazing Management And Feed Strategies To Alleviate Fescue Toxicosis, Glen Aiken

Kentucky Grazing Conference

Tall fescue grown in Kentucky is productive and persistent and that is why the state has 5 million acres of the stuff! Fescue has tolerances to drought, low fertility, and overgrazing that are attributed to a fungal endophyte which infects most tall fescue plants. Unfortunately, these advantages of tall fescue that livestock producers appreciate are offset by reduced calving percentages and calf weight gains caused by consumption of ergot alkaloids produced by the endophyte. Ergot alkaloids affect the physiology of cattle to cause elevation in body temperature, reduced sweating, retention of winter hair coats and uncontrollable growth of summer hair …

Herbicide Options For Pasture Weed Control, Jonathan D. Green

Kentucky Grazing Conference

Weeds have become more evident and problematic in grazed pastures as forage and livestock production systems have intensified. As animals consume the more desirable and palatable plant species in pastures, weedy plants become more abundant. In particular, animals tend to avoid grazing plants which contain thorns, prickly spines, or are unpalatable making some pastures less productive. In fact, many of the more problematic plants observed in Kentucky’s grazed pastures possess these characteristics including thistles, spiny amaranth (spiny pigweed), horsenettle, common cocklebur, tall ironweed, and buttercups.

Horse Pasture Monitoring Program: Results After Three Years, Tom Keene, S. Ray Smith

Kentucky Grazing Conference

Since 2001-2002 and the outbreak of Mare Reproductive Loss Syndrome (MLRS) the University of Kentucky has been developing stronger ties with the state’s equine industry. Approximately 30% of the foal crop was lost in the Central Bluegrass region during this period with a devastating impact on the industry. Traditionally, many Thoroughbred farms have functioned as independent entities, but MLRS helped them to realize that the University of Kentucky had tremendous amount of expertise that they could provide in the areas of animal health, forage management, and other areas. Initially, there were many theories as to the cause of MLRS with …

Forage Legumes Vs. Fertilizer Nitrogen, Garry D. Lacefield

Kentucky Grazing Conference

A few weeks after I started to work as a Forage Extension Specialist at the University of Kentucky, I heard my mentor and forage idol, Mr. Warren Thompson, speak on forage legumes. Warren, along with Dr’s. Tim Taylor and W.C. Templeton had pioneered the “Pasture Renovation Program” in Kentucky. In addition, Mr. Ed Smith had joined the team and collectively they developed the “Power-til Pasture Renovator.” Warren began his presentation by talking about Kentucky Forages pointing out we were so fortunate to have a good grass base and the opportunity to grow lots of legumes. He also talked about all …

Growth Of Grasses And Legumes: Impact On Grazing, S. Ray Smith

Kentucky Grazing Conference

The primary, cool season, perennial pasture grasses used in Kentucky are orchardgrass, bluegrass, and tall fescue. When not grazed or harvested, each of these passes through successive stages of growth in the spring: 1) leafy vegetative; 2) boot with seed heads enclosed in leaf sheath; 3) heading when the seed heads begin to show and, 4) bloom when pollination has occurred ( Figure 2). Since fiber and lignin contents increase steadily beyond the vegetative stage, while percent protein and digestibility decrease, a major goal in grazing management is to maintain these grasses in the leafy, vegetative stage at all times. …

Foreword And Kfgc Award Winners [2008], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, Jennifer D. Cotter, Andrew Storfer, Robert B. Page, Christopher K. Beachy, S. Randal Voss

Biology Faculty Publications

BACKGROUND: Very little is known about the immunological responses of amphibians to pathogens that are causing global population declines. We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV).

RESULT: At 0, 24, 72, and 144 hours post-infection, spleen and lung samples were removed for estimation of host mRNA abundance and viral load. A total of 158 up-regulated and 105 down-regulated genes were identified across all time points using statistical and fold level criteria. The presumptive functions of these genes suggest a robust innate immune …

Forage News [2008-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing Conference
• Grazing Crop Residues
• U.K. Forage Website
• KFGC Board Meeting Highlights
• Frost Brings Danger: Prussic Acid Poisoning
• Kentucky-Tennessee Cow-Calf Conference
• Dow Agrosciences Buys Dairyland Seed
• Court Decision Won’t Affect RR Alfalfa status
• Bale Feeder Reduces Waste
• Upcoming Events

Agricultural Situation And Outlook Fall 2008, Sara Williamson, Kenneth H. Burdine

Agricultural Situation and Outlook

No abstract provided.