Life Sciences Commons^™

Evaluation Of Fecal Egg Counts (Fec) As A Viable Means To Estimate Ascaridia Galli, Heterakis Gallinarum And Capillaria Obsignata Burdens In Laying Hens, Alexander H. Moss

Animal Science Undergraduate Honors Theses

The control of gastrointestinal helminths, specifically Ascaridia galli, Heterakis gallinarum, and Capillaria obsignata, is a critical component of poultry health management, particularly in free-range and organic farming systems. Methods of estimating parasite burdens, such as post-mortem examinations, are not feasible for large-scale commercial operations due to their invasiveness and time requirements. This study aimed to evaluate the use of fecal egg counts (FEC) as a non-invasive and efficient method for estimating the worm burdens of these parasites in laying hens. A flock of 12 brown egg laying, spent, hens were individually penned on concrete. Fecal samples (both cecal and intestinal) …

Dual-Task Cost Of Gait Measures In Individuals With And Without Parkinson's Disease, Addison G. Miller

Health, Human Performance and Recreation Undergraduate Honors Theses

Parkinson’s disease (PD), estimated to affect over one million people by 2030, is a neurodegenerative disease that damages the dopaminergic neurons located in the brain. Losing these neurons cause individuals with PD to experience impairments in basic motor and cognitive functions. Symptoms of PD often include bodily shaking, muscle stiffness, and abnormalities in gait and posture. Individuals with PD can also experience impairment when walking and performing secondary tasks, such as walking while talking, known as dual tasking. Dual tasking can lead to increased risk of falling or losing balance, as well as the inability to move freely while carrying …

Assessing The Implicit Prices Of Health And Wellness Product Attributes In Pet Food: A Hedonic Analysis Approach, Lonnie Hobbs Jr., Andrew Anderson

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Here we examine product attributes present in dry dog food to show there exist potential price premiums and discounts associated with health and wellness attributes in dry dog food. The findings indicate price premiums are associated with attributes related to digestion and allergy care. Pricing discounts are found to be associated with immune support and dental attributes. The results of this study are anticipated to be a starting point for more sophisticated and dynamic analysis of pricing and willingness-to-pay studies in the pet food industry.

Morphology Of Smallmouth Bass (Micropterus Dolomieu) In Arkansas, Robert Laroche

Biological Sciences Undergraduate Honors Theses

The Smallmouth Bass, Micropterus dolomieu, is one of the most important game fishes in Arkansas. Three distinct lineages are recognized from different river basins: the Northern, Neosho, and Ouachita Smallmouth Bass. Understanding the biodiversity within this species complex inhabiting different water bodies in Arkansas is important for effective management of the fisheries resource and to guide conservation efforts. But current data on distribution of distinct forms are insufficient to guide management. The primary goals of the study presented here was to quantify morphological characteristics to identify distinct lineages of Smallmouth Bass, and to determine their specific locations within Arkansas streams …

The Prevalence Of Cytauxzoon Felis In Southern United States, Anna Haynes

Biological Sciences Undergraduate Honors Theses

Cytauxzoonosis, caused by transmission from a tick infected with the pathogen Cytauxzoon felis, is considered an emerging tick-borne disease in domestic felines. While not as fatal in other vertebrates such as the reservoir host Lynx rufus (bobcat), in domestic cats, infection is fatal nearly 100% of the time. This study sought to screen ticks pulled from felines for the pathogen across three states in an area identified as a hotspot. Out of 80 ticks screened, 12 tested positive for the pathogen. The positive population of ticks came from 3 neighboring counties in Southern Missouri: Jasper, McDonald, and Newton. McDonald …

Growth Of Methanogens Using Pyrite As An Energy Source: Implications For Life On Mars, Mckenzie K. Ragan

Biological Sciences Undergraduate Honors Theses

The search for life beyond Earth has been the motivating factor for many scientist’s research in the past and currently. Specifically, the efforts have largely been focused on Mars. Mars exhibits qualities that are similar to the life-harboring characteristics of Earth, creating the possibility for life to exist on Mars. Mars’ subsurface conditions may be ideal for specific microorganisms to thrive, providing a haven away from the harsh surface conditions (Mancinelli, 2000). It is known that one of the most important elements to support life, H₂O is found in the subsurface of Mars, along with an atmosphere lacking …

Protein Intake And The Wellbeing Of Postmenopausal Women: Investigating Sleep, Mood, And Quality Of Life, Aleesha Imran

Biological Sciences Undergraduate Honors Theses

Postmenopausal women commonly experience declines in sleep quality, mood, and overall wellbeing, with limited non-pharmacological options available to address these concerns. This randomized controlled trial investigated whether daily beef consumption as part of a higher-protein diet could improve sleep, mood, and quality of life over four weeks. Participants were randomized to either a standard protein intake control group or a treatment group consuming a high-protein diet with daily lean beef.

The treatment group showed modest improvements in subjective sleep quality and perceived health compared to controls, although these changes were not statistically significant. Fatigue, however, significantly decreased in the treatment …

Metabolic Responses Of Adipose-Derived Stem Cells In Collagen I Hydrogels, Caroline R. King

Biological Sciences Undergraduate Honors Theses

Peripheral nerve injury affects approximately 44 in 1 million people in the United States every year and can lead to incomplete nerve recovery and loss of motor and sensory function. A significant amount of energy is required for nerve injury repair. A lack of energy allocation to the injury site hinders repair. Current treatments include hollow nerve guidance conduits, decellularized nerve grafts, and autografts. However, they are limited by factors such as donor site morbidity and sustained immunosuppressive treatments. Using three-dimensional collagen I hydrogels laden with adipose-derived stem cells (ASCs) can assist in neural regeneration. Three-dimensional collagen scaffolds aim to …

The Relationship Between Oxidative Stress And Partner Opposition In Conflict Resolution, Jenna Thomas

Biological Sciences Undergraduate Honors Theses

Given the inevitability of conflict in romantic relationships, understanding its biological impact is crucial for developing conflict resolution strategies that promote physical health. While many studies highlight relationship conflict’s negative effects on bodily systems, few examine how specific facets of conflict, such as opposition, impact the body. Partner opposition may contribute to oxidative stress, a biological marker associated with poor physical health, by activating the amygdala. This study investigated the association between partner opposition and oxidative stress in 126 couples expecting their first child. Participants attended a lab session where problem-solving discussions were recorded to assess oppositional behavior and urine …

Towards The Design Of A Super Fgf1-Fgf10 Chimera, Megan Brown, Suresh Thallapuranam

Biological Sciences Undergraduate Honors Theses

The Fibroblast Growth Factor family consists of 23 different fibroblast growth factors (FGFs) that are each involved in various cellular processes, including cell proliferation and tissue repair. Fibroblast growth factor receptors (FGFR) are different tyrosine kinase receptors. There are four FGFRs in the human body, each binding to different combinations of FGFs. Fibroblast growth factor 1 (FGF1) is the only FGF that can bind with all four types of fibroblast growth factor receptors. Therefore, it is the most powerful mitogen. However, FGF1 has low thermodynamic stability under the physiological conditions. Prior work in the Kumar lab has constructed several points …

The Expression Of Intestinal Tight Junction Proteins Induced By 1,25-Dihydroxyvitamin D3-Glycosides Supplementation To Reduce Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers, Kennedy M. Hicks

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a leading cause of lameness in broiler chickens, impacting animal welfare and commercial poultry production. Supplementation using organic trace minerals, vitamin D, probiotics, and prebiotics has been studied to mitigate BCO lameness incidences in broiler chickens. Our previous study demonstrated that feeding Panbonis®-G-1,25(OH)₂D₃ from Solanum glaucophyllum, containing 1 µg/kg G-1,25(OH)₂D₃, to broilers for the first 28 days of their production cycle reduced BCO lameness by approximately 50%. In this study, we investigated the impact of 1,25-dihydroxy vitamin D₃ supplementation on the expression of tight junction …

A Nano-Scale Approach To Controlled Apoptosis In Brain Cancer Through Magneto-Mechanical Actuation, Sakura Rieck

Biological Sciences Undergraduate Honors Theses

In this study, we investigate the effects of cell therapy and magneto-mechanical actuation (MMA) to treat glioblastoma (U87). MMA approach employs super low-frequency alternating magnetic fields (AMF) to actuate intracellular PEGylated superparamagnetic iron oxide nanoparticles (PEG-SPIONs). Magnetic forces are translated into mechanical agitation on PEG-SPIONs, which can disrupt key cellular components. We explored two ways in which the MMA approach can be used to combat cancer growth. First, we investigated MMA directly in U87 cells. Preliminary observations indicate that PEG-SPIONs are intracellularly taken up by the U87 cells via micropinocytosis. Cell-counting kit-8 toxicity assays revealed a cytotoxic effect of MMA …

Immune Function Modulation By Temperature On Isolated Rainbow Trout Macrophages, Abby Hunter

Biological Sciences Undergraduate Honors Theses

Fever is an evolutionary mechanism that enhances immune function, yet the molecular and metabolic responses to fever temperatures in ectothermic species remain poorly understood. In this study, we investigated how fever temperatures (19° C) influence macrophage function and metabolic activity in rainbow trout (Oncorhynchus mykiss), rather than normal temperatures (16° C). Using a rainbow trout monocytic cell line, RTS11, and primary head kidney-derived macrophages (HKCs), we examined the effects of fever temperatures on nitric oxide (NO) production, gene expression, protein synthesis, and energy metabolism. We found that fever temperatures increased NO production in both cell types, with further …

Negotiating Food Security, Maria Cudowska

Journal of Food Law & Policy

In the United States and worldwide, nutrition and food emerge as both high-profile public policy targets and as fundamental aspects of the right to self-determination. This Article examines how national security impacts food security in the United States. It explores conflicts and synergies between municipal, state, and federal law developments related to the right to food and investigates recent developments in how those commitments have been negotiated. The challenge and nebulous justiciability of the right to food can be in part attributed to existing global and national systems, which include supply chains, welfare programs, and foreign investment in the U.S. …

Bang!: Real-Time Measurements And Transcriptomics Of Metabolic Reprogramming In Activated Macrophages At Fever Temperature, Kenneth Howard Taylor

Graduate Theses and Dissertations

We investigated the real-time metabolic rates of activated macrophage cell lines derived from mouse, chicken and rainbow trout. Each species showed a different kinetic signature with metabolic rates accelerating under fever conditions specific to each organism. Upon activation mouse macrophages increased their rate of glycolysis early and then a later reprogramming phase occurred with a dramatic decrease in oxidative phosphorylation (OXPHOS) accompanied by a reciprocal second increase in glycolytic rates. Chicken macrophages showed an initial increase in both glycolytic and OXPHOS rates. The late drop OXPHOS rates also occurred in chicken however there was no reprogramming that occurred as glycolytic …

International Rice Outlook: International Rice Baseline Projections 2024–2034, A. Durand-Morat, W. Mulimbi

AAES Research Reports and Research Bulletins

• Global rice consumption is projected to exceed global rice production for most of the coming decade, with stocks playing a crucial role in balancing the global market over the projected period.

• The projected growth in production is almost exclusively due to productivity gains.

• The projected global rice consumption is growing, driven mainly by population growth. However, the average global per capita rice consumption is estimated to increase slowly in the coming decade due to a regain in per capita consumption in Asia, the largest rice-consuming region worldwide.

• The international prices of long-grain and medium-grain rice are …

Dental Microwear Of Miocene Primates From The Carpathian Basin, Rudabánya, Hungary, Anna K. Wilcox

Biological Sciences Undergraduate Honors Theses

This study reconstructs and compares the dietary habits of the late Miocene primates Anapithecus hernyaki and Rudapithecus hungaricus by analyzing dental microwear patterns on fossilized teeth recovered from Rudabánya, Hungary. Previous research suggested that these sympatric species were both primarily frugivores but were unable to separate them by diet. However, earlier studies were limited by smaller sample sizes and less advanced methodologies. This study used an expanded sample and modern microwear texture analysis techniques to compare molar and incisor microwear patterns between the two species. Results revealed that Rudapithecus molars exhibited higher complexity compared to Anapithecus molar complexity, suggesting a …

Assessment Of Nitrogen Source Utilization During Nitrogen Fixation And Nitrogenase Regulation By Methanosarcina Acetivorans, Avery Carter

Biological Sciences Undergraduate Honors Theses

Nitrogen is a crucial element in biomolecules such as proteins and DNA, essential for all cellular life. Biological nitrogen fixation, a process utilized by certain bacteria and archaea, reduces dinitrogen (N₂) to ammonia (NH₃), enabling growth in the absence of fixed nitrogen sources like NH₃. This process is driven by the metalloenzyme nitrogenase, which catalyzes the reaction: N₂ + 16ATP + 8e⁻ + 8H⁺ → 2NH₃ + H₂ + 16ADP + 16Pi. Due to its high energy demand, nitrogenase production and activity are tightly regulated. Among archaea, nitrogenase is found exclusively in methanogens, where its regulation remains poorly understood. We …

Phosphatase Activity And Structure Of The Spoiie Protein In Clostridioides Difficile, Kathryn Wootten

Biological Sciences Undergraduate Honors Theses

Clostridioides difficile is a major cause of severe gastrointestinal infections and diseases, particularly in healthcare settings. Because of its ability to produce infectious endospores by sporulation, the protein SpoIIE, which is known to play a role in the regulation of this process, is being explored as a potential therapeutic target. In order to gain better insight into the structure and function of this protein, a concentrated and purified sample of the protein must be obtained. The goal of this study was to determine the conditions that would yield the highest amount of SpoIIE. The density of the cultures of bacteria …

Relationship Between Nutritional Knowledge And Eating Disorder Risk Levels, Valeria Fernandez

Human Nutrition and Hospitality Management Undergraduate Honors Theses

The prevalence of disordered eating is a critical public health concern that often progresses towards the development of a diagnosable eating disorder (ED). This, in turn, has detrimental physical and psychological consequences for individuals. Organizations such as the American Society of Parenteral and Enteral Nutrition (ASPEN) and the Academy of Nutrition and Dietetics (AND) have recognized nutrition education as a crucial component of prevention and recovery. Determining the extent to which nutrition knowledge is related to risk factors for EDs is worth investigating due to its implications for healthcare approaches – especially among adolescent and young adult populations. The goal …

Quantifying Temperature Effects On Developmental Rate And Plant Quality Of Compact Container-Grown Tomato (Solanum Lycopersicum), Morgan Humphrey

Horticulture Undergraduate Honors Theses

Mean daily temperature effects on plant development rates and quality were evaluated for compact container-grown tomato (Solanum lycopersicum). Compact tomato varieties ‘Siam’ and ‘Red Velvet’ were grown in greenhouses at 18 to 26 °C (Experiment 1) and 20 to 30 °C (Experiment 2) under supplemental high-pressure sodium lighting and 16-hour photoperiod. The number of days to first open flower, first ripe fruit, and from flower to ripe fruit were measured and development rates calculated by taking the reciprocal (e.g. 1/days). Temperature effects were predicted by fitting a linear (for first open flower) and a nonlinear exponential function (for …

Designing And Installing A Disability Accessible Teaching Garden, Annabelle D. Denham

Horticulture Undergraduate Honors Theses

As horticulture becomes increasingly popular as a subject, activity, and form of therapy, its inclusivity has not kept up with the pace of its growth. In order to address the need for inclusivity and accessibility, gardens must adopt universal design. Individuals with special needs continuously face discrimination. While horticulture therapy has long been studied and catered to primarily neurotypical and able-bodied individuals, there is limited research on creating gardens specifically to include those with disabilities. This project aimed to create a garden that could serve the needs of all participants, regardless of their ability and sensory needs. The garden was …

Melanaphis Sacchari/Sorghi Complex: Current Status, Challenges And Integrated Strategies For Managing The Invasive Sap-Feeding Insect Pest Of Sorghum, Alejandro Vasquex, Joseph Edward Belsky, Neetu Khanal, Hena Puri, Devi Balakrishnan, Neelendra K. Joshi, Joe Louis, Glenn Studebaker, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Melanaphis sacchari (Zehntner;Hemiptera: Aphididae), sugarcane aphid (SCA), is an invasive phloem-feeder found worldwide with a wide host range of economically important plants including sorghum and sugarcane. Given its high reproductive capacity and ability to rapidly spread over long distances, SCA presents challenges for effective control, leading to substantial economic losses. Recent studies have identified two multiloci SCA genotypes specialized in feeding on sugarcane (MLL-D) and sorghum (MLL-F) in the USA, which raises concerns as the USA is the second largest sorghum-producing country. This has encouraged research towards identifying these two biotypes where some research has stated them as two species; …

Activity-Based Protein Profiling On Nucleophilic Amino Acid Residues With Pyrocarbonates, James Van Horn

Chemistry & Biochemistry Undergraduate Honors Theses

The complexity of the proteome requires strategies that efficiently characterize protein functions. Activity-Based Protein Profiling (ABPP) is a method that identifies enzyme activity by targeting key catalytic residues. This study focused on histidine, a residue involved in enzymatic catalysis, metal coordination, and protein-protein interactions. Despite its importance, histidine-specific chemical probes are limited due to its variable reactivity. This work developed and characterized a histidine-specific probe using a pyrocarbonate-based mechanism. Singleresidue assays confirmed selective reactivity of the probe with histidine over other nucleophilic amino acids. Further testing on enzymatic proteins and lysed cell proteomes showed that the probe modified histidine residues …

Genomic Insights Into Oxalate Content In Spinach: A Genome-Wide Association Study And Genomic Prediction Approach, Haizheng Xiong, Kenani Chiwina, Waltram Second Ravelombola, Yilin Chen, Ibtisam Alatawi, Qun Luo, Theresa M. Phiri, Beiquan Mou, Ainong Shi

Horticulture Faculty Publications and Presentations

Oxalate content in spinach is a key trait of interest due to its relevance to human health. Understanding the genetic basis of it can facilitate the development of spinach varieties with reduced oxalate levels. In pursuit of understanding the genetic determinants, a diverse panel comprising 288 spinach accessions underwent thorough phenotyping of oxalate content and were subjected to whole-genome resequencing, resulting in a comprehensive dataset encompassing 14 386 single-nucleotide polymorphisms (SNPs). Leveraging this dataset, we conducted a genome-wide association study (GWAS) to identify noteworthy SNPs associated with oxalate content. Furthermore, we employed genomic prediction (GP) via cross-prediction, utilizing five GP …

The Evolving Changes In The Regionalization Of Export Bans On U.S. Poultry Trade From The 2015 And 2022 Highly Pathogenic Avian Influenza Events, Kylie A. Roesler

Graduate Theses and Dissertations

As food security becomes a growing concern worldwide in a trading system dominated by globalization, the safeguarding of a bio-secure food system is at risk. One of the greatest risks to this stability of the system is, highly pathogenic avian influenza or HPAI which is growing in frequency and impact. Therefore, the global response to disease events is evolving into a newfound understanding of disease prevalence across the animal protein industries. This study will analyze the 2022 HPAI event in the United States regarding the regionalization of restrictions imposed by trading partners accounting for trade agreements executed. The examination of …

Physiological And Molecular Responses Of Diverse Rice Genotypes Under Drought Stress, Huda Mohmmed Alshaya

Graduate Theses and Dissertations

Climate change-induced drought stress is a significant constraint on global rice (Oryza sativa L.) production, threatening food security. This study evaluated the drought resilience of 15 diverse rice genotypes from the USDA mini-core collection under field, greenhouse, and osmotic stress conditions. Field trials assessed reproductive-stage drought tolerance based on panicle length (PL), number of spikelets per panicle (NSP), and spikelet sterility (SS). Greenhouse experiments examined moisture retention at the vegetative stage. Significant genotypic variation was observed, with genotypes 310724, 310779, 311181, 311603, 311793, and Vandana exhibiting drought tolerance through stable PL and SS. Additionally, genotypes 310100, 310428, 311255, N22, …

Comparison Analyses Of Consumer Acceptance, Evoked Emotions, And Purchase Intent Between Gluten-Containing Versus Gluten-Free Chocolate Chip Cookie Products, Shaelyn Frauenhoffer, Eniola Ola, Suzanne Jervis, Susan E. Gauch, Han-Seok Seo

Food Science Faculty Publications and Presentations

The demand for gluten-free products has increased significantly over the past few decades. This study aimed to determine differences in consumer acceptance, emotional responses, and purchase intent between gluten-containing versus gluten-free chocolate chip cookies. Twelve commercially available chocolate chip cookie products from 11 brands were evaluated, including 7 gluten-free and 5 gluten-containing options. Eighty-nine participants without celiac disease took part in this 2-day study, evaluating six cookies per day. Participants rated sensory acceptance (appearance, flavor, texture, and overall liking) using a 9-point hedonic scale and evaluated specific sensory attributes (chocolate flavor, sweetness, chewiness, and hardness) using a 5-point just-about-right (JAR) …

Structural Stability Of Fgf1-Fgf2 Dimer Established Via Salt Bridges With Mutation K296q, Ashleigh Letterman

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast Growth Factors (FGFs) are a family of cell-signaling proteins. FGF, specifically the FGF-1 subfamily, which contains FGF-1 and FGF-2, is important in several biological functions such as wound healing, cell proliferation, and angiogenesis. FGF-1 has cell proliferation properties and FGF-2 has angiogenic properties. These FGFs have great potential for wound-healing therapy medications...

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Identifying Microbial Complexities And Control Strategies At Pre-Harvest Poultry Production, Tolulope Tiwa Ogundipe

Graduate Theses and Dissertations

While significant progress has been made in post-harvest control of Salmonella in the poultry industry, the development of effective pre-harvest intervention strategies remains critical for reducing foodborne pathogens. Environmental reservoirs such as poultry litter and drinking water systems (DWS) are potential harborage sites for Salmonella and other microbial communities. Understanding the microbiota of these environments, including biofilms within water lines, is essential to identifying factors contributing to Salmonella colonization and improving pre-harvest control approaches. This study aimed to evaluate the incidence and quantity of Salmonella in poultry litter and water lines, characterize the microbial populations in these environments, and assess …